CN104306322A - Cordyceps-militaris-ingredient contained eye cream and preparing method of cordyceps-militaris-ingredient contained eye cream - Google Patents
Cordyceps-militaris-ingredient contained eye cream and preparing method of cordyceps-militaris-ingredient contained eye cream Download PDFInfo
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Abstract
The invention discloses cordyceps-militaris-ingredient contained eye cream. The cordyceps-militaris-ingredient contained eye cream contains twenty-one kinds of active ingredients including hydrolyzed avenin, aloe fermentation objects, cordyceps militaris extract, purslane extract, persimmon leaf extract, orange peel extract, scutellaria root extract, phellinus igniarius extract, white willow bark extract, vanilla extract, cold-resistant fragrant rose extract, green bean extract, green tea extract, tricholoma matsutake extract, ginseng extract, astragalus membranaceus root extract, rhodiola rosea root extract, rice bran extract, jasmine flower extract, peach kernel extract and ho leaf extract, and further contains asiaticoside. The cordyceps-militaris-ingredient contained eye cream has the advantages of being safe, nontoxic and resistant to wrinkles. The invention further provides a preparing method of the cordyceps-militaris-ingredient contained eye cream. The preparing method is simple, and the production cost is effectively reduced.
Description
Technical field
The present invention relates to cosmetic field, particularly relate to a kind of eye cream containing Cordyceps militaris (L.) Link. composition and preparation method thereof.
Background technology
Cordyceps militaris (L.) Link. formal name used at school Cordyceps militaris (L.) Link. (
cordyceps militaris), it and Cordyceps of Qinghai Province belong to Eumycota Ascomycotina Cordyceps, therefore, are called belonging to together " brother " of Cordyceps by expert.The perfect stage of Cordyceps militaris (L.) Link. life cycle comprises mycelial growth and sporophore growth.The insect larvaes such as Lepidoptera, coleoptera, Diptera or pupa infect the spore of Paecilomyces varioti, and when environmental condition is suitable for, spore just sprouts formation mycelium, and mycelium draws the nutrition in worm (pupa) body as the material of its growth promoter and the energy.After mycelia decomposes the various tissue in worm (pupa) body and organ, hyphostoma development transfers reproductive growth to by nourishing and growing, mycelium twist together gradually be differentiated to form sclerotium and pass that the external top becoming crocus or Chinese red is slightly expanded, in bar-shaped Stroma (sporophore), the many heads from polypide of Stroma, chest, caudad etc. stretch out.
Wild Cordyceps militaris (L.) Link. is similar to traditional Cordyceps, very rare at occurring in nature.Most research is thought, Cordyceps militaris (L.) Link. is close with natural cordyceps on active ingredient, and its medicinal and edibility can match in excellence or beauty with natural Cordyceps.The Cordyceps militaris (L.) Link. cellulose content of factorial praluction is higher than natural cordyceps, and the content of its protein, aminoacid, vitamin and trace element has has all met or exceeded the level of natural cordyceps.Plant in Cordyceps reported more than 350, Cordyceps militaris (L.) Link. is the rare Cordyceps that can form a large amount of cordycepin.Because with Cordyceps sibship recently, effective ingredient is quite even higher, and can artificial culture, Cordyceps militaris (L.) Link. becomes the Ideal Substitute of natural cordyceps.Riched Cordyceps Militaris is containing various bioactivators, as Cordyceps polysaccharide, cordycepin, cordycepic acid, SOD enzyme, adenosine, polypeptide/aminoacid, mannitol etc., with the cosmetics that Cordyceps militaris (L.) Link. extract is made for raw material, then there is antioxidation, defying age and the effect such as nutrition, check melanin synthesis is provided, extra-nutrition can be produced to human skin, improve coarse, effect such as enhancing elasticity, moisturizing whitening etc.
To increase due to the age and the various factors such as seasonal variations, ultraviolet radiation, smoking, operating pressure are large, sitting before computer, television set, contact radioactive substance causes the skin problem of people, as skin corium subside, moisture-retaining capacity declines, xerosis cutis is aging.Therefore, cosmetics have become the requisite articles for daily use of one of people.Eye cream is used to this thinner one deck skin of protection around eyes, and some effects, except can lowering the problem of black eye, eye pouch, also possess moist, to improve wrinkle and microgroove effect.The kind of eye cream is roughly divided into eye mask, eye glue, eye gel, eye sticker, eye cream; Functionally be divided into moist eye cream, consolidation eye cream, aging resistance eye cream, antiallergic eye cream etc.At present, mostly with the addition of some chemical compositions inside in commercially available eye cream, consumer is after life-time service similar products, and skin may occur anaphylaxis.Therefore, need exploitation badly a kind of containing large number of biological active component, existing good result of use, the healthy eye cream of consumer can not be damaged again.
Summary of the invention
For the problems referred to above, the invention provides a kind of eye cream containing Cordyceps militaris (L.) Link. composition and preparation method thereof, there is safety non-toxic, crease-resistant feature, and production cost is lower.
For realizing above-mentioned purpose of the present invention, the invention provides a kind of eye cream containing Cordyceps militaris (L.) Link. composition, is formulated by weight by A, B1, B2, C and D component raw material.
Component A: 16, octadecanol and cetyl glucoside (MONTANOV 68) 1.85-2.5 part, emulsifying agent A-165 0.1-0.16 part, cetearyl alcohol 1.1-1.6 part, Jojoba seed oil 2.3-3.75 part, Fructus oleae europaeae fruit oil 1.35-3.2 part, squalane 2-3 part, polydimethylsiloxane 0.65-1.4 part, tocopherol acetas 0.4-0.6 part, coenzyme Q10 0.042-0.058 part, ethylhexyl glycerol 0.2-0.5 part, plant hormone 0.08-0.14 part, butanediol 4-5.5 part, class ceramide 0.2-0.28 part, asiaticoside 0.15-0.35 part, plant sterol 0.4-0.55 part.
B1 component: xylitol 1.5-2.5 part, allantoin 0.13-0.28 part, nicotiamide 0.08-0.13 part, Aloe fermented product 0.25-0.35 part, hydrolysis avenin 0.1-0.4 part, trimethyl glycine 2-3.5 part, deionized water 42-60 part.
B2 component: glycerol 2.2-3.5 part, carboxymethyl R-glucan 0.12-0.2 part.
Component C: Nipagin ester 0.42-0.58 part, ring poly dimethyl oxosilane 2-3.75 part, polyacrylate-13 0.5-0.7 part.
D component: betanin 2.1-3.6 part, pentanediol 0.04-0.06 part, essence 0.01-0.035 part, plant bean ferment 0.1-0.25 part, Semen phaseoli radiati extract 0.6-0.85 part, Cordyceps extract 2.7-3.3 part, Herba portulacae extract 0.1-0.3 part, Folium Kaki extract 0.1-0.3 part, orange peel extract 0.01-0.1 part, scutellariae,radix extract 0.01-0.05 part, phellinus linteus extract 0.01-0.03 part, Salix Babylonica L.P.E 0.01-0.03 part, vanilla extract 0.01-0.03 part, Lengxiang rose flower extract 0.01-0.05 part, green tea extract 0.01-0.03 part, tricholoma matsutake 0.01-0.03 part, Radix Ginseng extract 0.01-0.03 part, Radix Astragali root extract 0.01-0.03 part, Rhodiola rosea L. root extract 0.01-0.1 part, rice bran extract 0.01-0.02 part, Flos Jasmini Sambac extract 0.01-0.02 part, Semen Persicae extract 0.01-0.03 part, Lignum cinnamomi camphorae leaf extract 0.01-0.02 part.
In described D component, the preparation method of Cordyceps extract is as follows.
The supersound extraction of Cordyceps polysaccharide in mycelium fermentation broth.
(1) liquid submerged fermentation mode is adopted to obtain cordyceps mycelium.
The technique of Cordyceps militaris (L.) Link. deep fermentation is as follows: fermentation medium consists of: 20g/L sucrose+2g/L Semen Maydis pulp+8g/L bacillus cereus tropina+0.5g/L MgSO
47H
2o+1g/L KH
2pO
4, adopt two-stage control pH strategy, control ph constant a certain numerical value between 6.0-7.0 when namely starting, after inoculation Cordyceps militaris strain, pH value constant control is a certain numerical value between 5.0-6.0 by 40h-60h again.Ventilation in fermentation tank controls between 1:0.3-l:0.6 that (unit is m
3/ m
3/ min), fermentation tank mixing speed controls at 120-200r/min.
(2) supersound extraction of mycelium fermentation broth.
Get the above-mentioned cordyceps mycelium fermentation liquid 100-200ml obtained through submerged fermentation, ultrasonic (59Hz) processes 15-45min, fermentation liquor collected after centrifugation centrifuged supernatant after supersound process, gained primary deposition thing adds 30-100ml alkali ion water again and carries out twice ultrasonic process, is total to supersound process three times successively.Merge three centrifuged supernatant, and with Rotary Evaporators (30 DEG C-50 DEG C, revolution 60rpm) concentrated solution of concentrated 20-30ml, add the dehydrated alcohol of concentrated solution 3 times amount, under 4 DEG C of conditions, precipitate 8-12h, incline and supernatant, precipitation absolute ethanol washing, drying, obtain polysaccharide crude, be Cordyceps extract.
Described alkali ion water is by carrying out electrolysis generation by pure water.
Described alkali ion water index is pH:9.80-13.80, current potential: >=-120mV.
The preparation method of described B1 component Aloe fermented product is as follows.
(1) surface cleaning: Fresh leaf of aloe clear water wash clean, described clear water can not use the tap water containing bleach, because bleach can cause aloe liquid variable color, go bad.
(2) ultraviolet radiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 5-30min.
(3) remove leaf skin: in sterilizing room, remove Fresh leaf of aloe leaf skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum.
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 10000-30000, time 0.2-5 minute); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice.
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product.
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10%(percent by volume of bacterium liquid).Fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Aloe gel fermented product.
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10%(percent by volume of bacterium liquid).Fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Folium Aloe fermented product.
Described Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, the preparation method of Lignum cinnamomi camphorae leaf extract is: get pulverous Herba Portulacae respectively, Folium Kaki, orange peel, scutellariae,radix, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Trichotoma matsutake, Radix Ginseng, Radix Astragali root, Rhodiola rosea L. root, Testa oryzae, Flos Jasmini Sambac, Semen Persicae, Lignum cinnamomi camphorae leaf, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, namely obtaining Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract respectively.
The present invention also provides a kind of preparation method of the eye cream containing Cordyceps militaris (L.) Link. composition, comprises the following steps.
Step one, component A added the first blending tank and stir and be warming up to 80-90 DEG C and keep 20 minutes.
Step 2, B1 and B2 component raw material added the second blending tank and stir intensification 80 DEG C and keep 20 minutes.
Step 3, the first blending tank Raw suction homogenizing tank to be stirred, then the raw material in the second blending tank to be added in homogenizing tank.
Step 4, emulsifying, after 5 minutes, stir and are cooled to 60 DEG C.
Step 5, to add component C raw material stirring even, continues to be cooled to 45 DEG C.
Step 6, add after D component raw material stirs and be cooled to 35-38 DEG C, ageing 24 hours.Fill, subpackage is also packed, and is finished product.
Compared with prior art beneficial effect of the present invention.
The invention provides a kind of eye cream containing Cordyceps militaris (L.) Link. composition and preparation method thereof, the described eye cream containing Cordyceps militaris (L.) Link. composition contains hydrolysis avenin, Aloe fermented product, Cordyceps extract, Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract 21 kinds of active component, also containing asiaticoside.Wherein Aloe fermented product has elimination scytitis and antibacterial action, and can significantly improve the anti-wrinkle effect of eye cream, is especially applicable to middle and aged women and uses.Herba Portulacae contains abundant SL3 fatty acid and vitamin A sample material.SL3 fatty acid forms the necessary material of cell membrane; Vitamin A sample substance maintains epithelial tissue as the normal function of skin, cornea.Orange peel extract contains terpenoid substance, plays antibacterial and natural perfume material.Containing a large amount of tannic acid in Folium Kaki, can effectively kill various antibacterial and fungus, there is effect that is clean and skin care.Hydrolysis avenin is rich in hydrolyzed protein, plays instant convergence and level and smooth effect; Scutellariae,radix extract is rich in flavone compound, has the effects such as antibacterial and contraction.Flos Jasmini Sambac extract has antioxidation and bacteria resistance function.In addition, adopt liquid submerged fermentation mode to obtain cordyceps mycelium in the present invention, have that cultivation cycle is short, active substance is easy to the advantages such as extraction, the active matter content of a lot of medicinal fungi liquid fermentation is far above the active matter content of tame medicinal fungi; Add the tropina of bacillus cereus in mycelium fermentation broth, both improved the output of cordyceps mycelium, again saved cost of material.
Detailed description of the invention
The present invention is further described below in conjunction with specific embodiment.
The invention provides a kind of eye cream containing Cordyceps militaris (L.) Link. composition, is formulated by weight by following A, B1, B2, C and D component raw material.
Component A: 16, octadecanol and cetyl glucoside (MONTANOV 68) 1.96 parts, emulsifying agent A-165 0.13 part, cetearyl alcohol 1.2 parts, Jojoba seed oil 2.5 parts, Fructus oleae europaeae fruit oil 2.2 parts, squalane 2.5 parts, polydimethylsiloxane 0.75 part, tocopherol acetas 0.5 part, coenzyme Q10 0.05 part, ethylhexyl glycerol 0.34 part, plant hormone 0.12 part, butanediol 4.7 parts, class ceramide 0.25 part, asiaticoside 0.2 part, plant sterol 0.45 part.
B1 component: xylitol 1.6 parts, allantoin 0.21 part, nicotiamide 0.09 part, Aloe fermented product 0.3 part, hydrolysis avenin 0.3 part, trimethyl glycine 2.4 parts, deionized water 53 parts.
B2 component: glycerol 3 parts, carboxymethyl R-glucan 0.15 part.
Component C: Nipagin ester (PHENONIP) 0.5 part, ring poly dimethyl oxosilane 2.75 parts, polyacrylate-13(SEPIPLUS 400) 0.61 part.
D component: betanin 2.4 parts, pentanediol 0.05 part, 0.01 part, essence, plant 0.2 part, bean ferment, Semen phaseoli radiati extract 0.65 part, Cordyceps extract 3 parts, Herba portulacae extract 0.2 part, Folium Kaki extract 0.1 part, orange peel extract 0.05 part, scutellariae,radix extract 0.03 part, phellinus linteus extract 0.01 part, Salix Babylonica L.P.E 0.02 part, vanilla extract 0.02 part, Lengxiang rose flower extract 0.04 part, green tea extract 0.02 part, tricholoma matsutake 0.02 part, Radix Ginseng extract 0.01 part, Radix Astragali root extract 0.01 part, Rhodiola rosea L. root extract 0.05 part, rice bran extract 0.01 part, Flos Jasmini Sambac extract 0.015 part, 0.01 part, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract 0.01 part.
In described D component, the preparation method of Cordyceps extract is as follows.
The supersound extraction of Cordyceps polysaccharide in mycelium fermentation broth.
(1) liquid submerged fermentation mode is adopted to obtain cordyceps mycelium.
Wild cordyceps militaris is at the Pupa bombycis tumor growth containing a large amount of insect protein, the present invention adopt add with Pupa bombycis insect protein closer to bacillus cereus tropina, cordyceps mycelium fermentation production rate is obtained and improves.The detailed process of Cordyceps militaris (L.) Link. deep fermentation is as follows: fermentation medium consists of: 20g/L sucrose+2g/L Semen Maydis pulp+8g/L bacillus cereus tropina+0.5g/L MgSO
47H
2o+1g/L KH
2pO
4, adopt two-stage control pH strategy, when namely starting, control ph is constant in 6.3, inoculation Cordyceps militaris strain (this liquid spawn by Cordyceps militaris (L.) Link. (
cordyceps militaris) fermentation) and afterwards 50h be 5.7 by pH value constant control again.Ventilation in fermentation tank controls at 1:0.4 that (unit is m
3/ m
3/ min), fermentation tank mixing speed controls at 180r/min.
(2) supersound extraction of mycelium fermentation broth.
Get the above-mentioned cordyceps mycelium fermentation liquid 100ml obtained through submerged fermentation, ultrasonic (59Hz) processes 30min, fermentation liquor collected after centrifugation centrifuged supernatant after supersound process, gained precipitate adds 100ml alkali ion water again and carries out twice ultrasonic extraction, is total to supersound extraction three times successively.Merge three centrifuged supernatant and use Rotary Evaporators (40 DEG C, revolution 60rpm) concentrated solution of concentrated 30ml, add the dehydrated alcohol of concentrated solution 3 times amount, 10h is precipitated under 4 DEG C of conditions, incline and supernatant and obtain precipitate, this precipitate absolute ethanol washing, drying, obtain polysaccharide crude, be Cordyceps extract.
Described alkali ion water is by carrying out electrolysis generation by pure water; Described pure water is after electrolysis, and enrichment hydroxide ion in water near negative electrode, in alkalescence, obtains alkali ion water.
The alkali ion water index that described alkali ion water outlet produces is pH:9.80-13.80, current potential: >=-120mv.
With distilled water extraction cordyceps mycelium effective ingredient, extraction efficiency is 16.5%, and the extraction efficiency of alkali ion water is 23.9%.As can be seen here, described alkali ion water extraction cordyceps mycelium effective ingredient has not only increased substantially extraction efficiency, also avoid the pollution because using chemical solvent to bring.
The preparation method of described B1 component Aloe fermented product is as follows.
(1) surface cleaning: first Fresh leaf of aloe clear water wash clean, described clear water can not use the tap water containing bleach, because bleach can cause aloe liquid variable color, go bad.
(2) ultraviolet irradiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 6min.
(3) remove leaf skin: in sterilizing room, remove Folium Aloe skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum.
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 20000, time 3 minutes); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice.
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product.
1. be, with aloe gel juice culture medium, with Hansenula anomala (lot number: AS2.297, Bei Nuo bio tech ltd, Shanghai) for inoculation strain, inoculum concentration 4%(percent by volume).Fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Aloe gel fermented product.
2., with Folium Aloe natural juice for culture medium, with Hansenula anomala (lot number: AS2.297, Bei Nuo bio tech ltd, Shanghai) for inoculation strain, inoculum concentration 4%(percent by volume).Fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Folium Aloe fermented product.
Prior art commonly uses Aloe original juice, and the present invention adopts Aloe fermented product, and this Aloe fermented product has following benefit compared with the Aloe original juice not through fermenting: composition is more various and molecule is less, thus is more easily absorbed by the skin.
Described Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, the preparation method of Lignum cinnamomi camphorae leaf extract is: get pulverous Herba Portulacae respectively, Folium Kaki, orange peel, scutellariae,radix, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Trichotoma matsutake, Radix Ginseng, Radix Astragali root, Rhodiola rosea L. root, Testa oryzae, Flos Jasmini Sambac, Semen Persicae, Lignum cinnamomi camphorae leaf, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, namely obtaining Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract respectively.
The present invention also provides a kind of preparation method of the eye cream containing Cordyceps militaris (L.) Link. composition, comprises the following steps.
Step one, component A raw material added the first blending tank and stir and be warming up to 80 DEG C and keep 20 minutes.
Step 2, B1 and B2 component raw material added the second blending tank and stir intensification 80 DEG C and keep 20 minutes.
Step 3, the first blending tank Raw suction homogenizing tank to be stirred, then the raw material in the second blending tank to be added in homogenizing tank.
Step 4, emulsifying, after 5 minutes, stir and are cooled to 60 DEG C.
Step 5, to add component C raw material stirring even, continues to be cooled to 45 DEG C.
Step 6, add after D component raw material stirs and be cooled to 35 DEG C, ageing 24 hours, fill, subpackage is also packed, and is finished product.
The efficacy assessments of the present embodiment formula composition and preparation method thereof gained eye cream product.
1, practical effect evaluation: participating in eye cream effect evaluation and test interviewee number is 33 people, using the present embodiment containing eye cream every day each 1 time sooner or later of Cordyceps militaris (L.) Link. composition, 30 days is a course for the treatment of.Use the present embodiment containing after eye cream one course for the treatment of of Cordyceps militaris (L.) Link. composition, objective evaluation is made to this eye cream moisturizing, moisturizing, trap, stretchability, viscosity, abnormal smells from the patient, often kind of effect judgment criteria is divided into third gear, wherein moisturizing: first grade is that moistening effect is very good, second gear is that moistening effect is general, and third gear is that moistening effect is bad; Moisturizing: first grade for moisturizing effect clearly, second gear is that moisturizing effect is general, and third gear is that moisturizing effect is bad; Trap: first grade is easy to absorb for elite, and second gear is that elite assimilation effect is general, and third gear is that elite assimilation effect is bad; Stretchability: first grade is that stretchability is very good, second gear is that stretchability is general, and third gear is that stretchability is bad; Viscosity: first grade is that viscosity is very good, second gear is that viscosity is general, and third gear is that viscosity is bad; Abnormal smells from the patient: first grade is natural comfortable, second gear is that chemical reagent is obvious, and third gear is not for having peculiar taste; Greasy feeling: first grade is salubrious, and second gear is general, and third gear is greasy.Concrete evaluation result is in table 1.
Table 1: the present invention evaluates containing the eye cream result of use of Cordyceps militaris (L.) Link. composition.
| ? | First grade | Second gear | Third gear |
| Moisturizing | 0 | 15 | 18 |
| Moisturizing | 6 | 12 | 12 |
| Trap | 6 | 27 | 0 |
| Stretchability | 18 | 11 | 4 |
| Viscosity | 6 | 27 | 0 |
| Abnormal smells from the patient | 20 | 0 | 13 |
| Greasy feeling | 0 | 1 | 32 |
As shown in Table 1, the present invention has good moisturizing, moisturizing, nutrition, convergence effect containing the eye cream of Cordyceps militaris (L.) Link. composition.
2, moisture-keeping efficacy evaluation: moisture-keeping efficacy evaluation adopts external weight method, according to the difference of cosmetic composition moisture absorption, performance of keeping humidity, different wetting agent molecules is different to the active force of hydrone, absorbs moisture different with keeping the ability of moisture.Hygroscopic effect power is large, strong to water molecules power, and absorb and keep the amount of moisture also larger, closure is good, also lacking of moisture loss.Utilize adhesive tape to imitate the biomaterial such as horny layer, epidermis, cosmetics smeared by adhesive tape, the application feature of simulation pragmatize cosmetic.Under the condition of constant temperature and humidity after certain hour (4 hours), weigh sample and place the of poor quality of front and back, obtain the loss of sample size, the effect of moisturizing ingredient moisturizing can be calculated.
Directly measure for sample with the eye cream of the present embodiment containing Cordyceps militaris (L.) Link. composition, determine that this moisturizing rate containing the eye cream of Cordyceps militaris (L.) Link. composition is about 43.48% by experiment.
3, senile-resistant efficacy evaluation: senile-resistant efficacy evaluation adopts in-vitro evaluation method, and working sample is to the Scavenging activity of free radical.Comprise three indexs, be respectively: remove the mensuration of DPPH ability, remove the mensuration of superoxide anion ability and the mensuration of scavenging hydroxyl ability.
In the mensuration removing DPPH ability, the present embodiment is 24.3%-39.32% containing the clearance rate of the eye cream of Cordyceps militaris (L.) Link. composition; In the mensuration removing superoxide anion ability, the present embodiment is 25.59%-35.67% containing the eye cream of Cordyceps militaris (L.) Link. composition to the suppression ratio of superoxide anion; In the mensuration of scavenging hydroxyl ability, the present embodiment is 16.53%-27.35% containing the eye cream of Cordyceps militaris (L.) Link. composition to the clearance rate of hydroxy radical.
4, pH value measures: take eye cream sample 1g(and be accurate to 0.1g), adding distil water 10g, is heated to 40 DEG C, and is constantly stirred to evenly, measures after being cooled to room temperature with pH meter.
Mensuration the present embodiment is 6.7-7.0 containing the pH value of the eye cream of Cordyceps militaris (L.) Link. composition.
Claims (5)
1., containing an eye cream for Cordyceps militaris (L.) Link. composition, be formulated by A, B1, B2, C and D component raw material:
Component A: 16, octadecanol and cetyl glucoside 1.85-2.5 part, emulsifying agent A-165 0.1-0.16 part, cetearyl alcohol 1.1-1.6 part, Jojoba seed oil 2.3-3.75 part, Fructus oleae europaeae fruit oil 1.35-3.2 part, squalane 2-3 part, polydimethylsiloxane 0.65-1.4 part, tocopherol acetas 0.4-0.6 part, coenzyme Q10 0.042-0.058 part, ethylhexyl glycerol 0.2-0.5 part, plant hormone 0.08-0.14 part, butanediol 4-5.5 part, class ceramide 0.2-0.28 part, asiaticoside 0.15-0.35 part, plant sterol 0.4-0.55 part,
B1 component: xylitol 1.5-2.5 part, allantoin 0.13-0.28 part, nicotiamide 0.08-0.13 part, Aloe fermented product 0.25-0.35 part, hydrolysis avenin 0.1-0.4 part, trimethyl glycine 2-3.5 part, deionized water 42-60 part;
B2 component: glycerol 2.2-3.5 part, carboxymethyl R-glucan 0.12-0.2 part;
Component C: Nipagin ester 0.42-0.58 part, ring poly dimethyl oxosilane 2-3.75 part, polyacrylate-13 0.5-0.7 part;
D component: betanin 2.1-3.6 part, pentanediol 0.04-0.06 part, essence 0.01-0.035 part, plant bean ferment 0.1-0.25 part, Semen phaseoli radiati extract 0.6-0.85 part, Cordyceps extract 2.7-3.3 part, Herba portulacae extract 0.1-0.3 part, Folium Kaki extract 0.1-0.3 part, orange peel extract 0.01-0.1 part, scutellariae,radix extract 0.01-0.05 part, phellinus linteus extract 0.01-0.03 part, Salix Babylonica L.P.E 0.01-0.03 part, vanilla extract 0.01-0.03 part, Lengxiang rose flower extract 0.01-0.05 part, green tea extract 0.01-0.03 part, tricholoma matsutake 0.01-0.03 part, Radix Ginseng extract 0.01-0.03 part, Radix Astragali root extract 0.01-0.03 part, Rhodiola rosea L. root extract 0.01-0.1 part, rice bran extract 0.01-0.02 part, Flos Jasmini Sambac extract 0.01-0.02 part, Semen Persicae extract 0.01-0.03 part, Lignum cinnamomi camphorae leaf extract 0.01-0.02 part,
In described D component, the preparation method of Cordyceps extract is:
(1) liquid submerged fermentation mode is adopted to obtain cordyceps mycelium:
The technique of Cordyceps militaris (L.) Link. deep fermentation is as follows: fermentation medium consists of: 20g/L sucrose+2g/L Semen Maydis pulp+8g/L bacillus cereus tropina+0.5g/L MgSO
47H
2o+1g/L KH
2pO
4, adopt two-stage control pH strategy, control ph constant a certain numerical value between 6.0-7.0 when namely starting, after inoculation Cordyceps militaris strain, pH value constant control is a certain numerical value between 5.0-6.0 by 40h-60h again; Ventilation in fermentation tank controls between 1:0.3-l:0.6 that (unit is m
3/ m
3/ min), fermentation tank mixing speed controls at 120-200r/min;
(2) supersound extraction of mycelium fermentation broth: get the above-mentioned cordyceps mycelium fermentation liquid 100-200ml obtained through submerged fermentation, ultrasonic (59Hz) processes 15-45min, fermentation liquor collected after centrifugation centrifuged supernatant after supersound process, gained primary deposition thing adds 30-100ml alkali ion water again and carries out twice ultrasonic process, is total to supersound process three times successively; Merge three centrifuged supernatant, and with Rotary Evaporators (30 DEG C-50 DEG C, revolution 60rpm) concentrated solution of concentrated 20-30ml, add the dehydrated alcohol of concentrated solution 3 times amount, under 4 DEG C of conditions, precipitate 8-12h, incline and supernatant, precipitation absolute ethanol washing, drying, obtain polysaccharide crude, be Cordyceps extract;
The preparation method of described B1 component Aloe fermented product is:
(1) surface cleaning: Fresh leaf of aloe clear water wash clean, described clear water can not use the tap water containing bleach, because bleach can cause aloe liquid variable color, go bad;
(2) ultraviolet radiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 5-30min;
(3) remove leaf skin: in sterilizing room, remove Fresh leaf of aloe leaf skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum;
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 10000-30000, time 0.2-5 minute); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice;
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product;
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10% of bacterium liquid, fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Aloe gel fermented product;
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10% of bacterium liquid, fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Folium Aloe fermented product;
Described Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, the preparation method of Lignum cinnamomi camphorae leaf extract is: get pulverous Herba Portulacae respectively, Folium Kaki, orange peel, scutellariae,radix, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Trichotoma matsutake, Radix Ginseng, Radix Astragali root, Rhodiola rosea L. root, Testa oryzae, Flos Jasmini Sambac, Semen Persicae, Lignum cinnamomi camphorae leaf, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, namely obtaining Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract respectively.
2. the eye cream containing Cordyceps militaris (L.) Link. composition as claimed in claim 1, it is characterized in that, be formulated by A, B1, B2, C and D component raw material:
Component A: 16, octadecanol and cetyl glucoside 1.96 parts, emulsifying agent A-165 0.13 part, cetearyl alcohol 1.2 parts, Jojoba seed oil 2.5 parts, Fructus oleae europaeae fruit oil 2.2 parts, squalane 2.5 parts, polydimethylsiloxane 0.75 part, tocopherol acetas 0.5 part, coenzyme Q10 0.05 part, ethylhexyl glycerol 0.34 part, plant hormone 0.12 part, butanediol 4.7 parts, class ceramide 0.25 part, asiaticoside 0.2 part, plant sterol 0.45 part;
B1 component: xylitol 1.6 parts, allantoin 0.21 part, nicotiamide 0.09 part, Aloe fermented product 0.3 part, hydrolysis avenin 0.3 part, trimethyl glycine 2.4 parts, deionized water 53 parts;
B2 component: glycerol 3 parts, carboxymethyl R-glucan 0.15 part;
Component C: Nipagin ester 0.5 part, ring poly dimethyl oxosilane 2.75 parts, polyacrylate-13 0.61 parts;
D component: betanin 2.4 parts, pentanediol 0.05 part, 0.01 part, essence, plant 0.2 part, bean ferment, Semen phaseoli radiati extract 0.65 part, Cordyceps extract 3 parts, Herba portulacae extract 0.2 part, Folium Kaki extract 0.1 part, orange peel extract 0.05 part, scutellariae,radix extract 0.03 part, phellinus linteus extract 0.01 part, Salix Babylonica L.P.E 0.02 part, vanilla extract 0.02 part, Lengxiang rose flower extract 0.04 part, green tea extract 0.02 part, tricholoma matsutake 0.02 part, Radix Ginseng extract 0.01 part, Radix Astragali root extract 0.01 part, Rhodiola rosea L. root extract 0.05 part, rice bran extract 0.01 part, Flos Jasmini Sambac extract 0.015 part, 0.01 part, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract 0.01 part,
In described B1 component, the preparation method of Cordyceps extract is:
(1) liquid submerged fermentation mode is adopted to obtain cordyceps mycelium:
The detailed process of Cordyceps militaris (L.) Link. deep fermentation is as follows: fermentation medium consists of: 20g/L sucrose+2g/L Semen Maydis pulp+8g/L bacillus cereus tropina+0.5g/L MgSO
47H
2o+1g/L KH
2pO
4, adopt two-stage control pH strategy, when namely starting, control ph is constant in 6.3, and after inoculation Cordyceps militaris strain, pH value constant control is 5.7 by 50h again; Ventilation in fermentation tank controls at 1:0.4 that (unit is m
3/ m
3/ min), fermentation tank mixing speed controls at 180r/min;
(2) supersound extraction of mycelium fermentation broth: get the above-mentioned cordyceps mycelium fermentation liquid 100ml obtained through submerged fermentation, ultrasonic (59Hz) processes 30min, fermentation liquor collected after centrifugation centrifuged supernatant after supersound process, gained precipitate adds 100ml alkali ion water again and carries out twice ultrasonic extraction, is total to supersound extraction three times successively; Merge three centrifuged supernatant and use Rotary Evaporators (40 DEG C, revolution 60rpm) concentrated solution of concentrated 30ml, add the dehydrated alcohol of concentrated solution 3 times amount, 10h is precipitated under 4 DEG C of conditions, incline and supernatant and obtain precipitate, this precipitate absolute ethanol washing, drying, obtain polysaccharide crude, be Cordyceps extract;
The preparation method of described B1 component Aloe fermented product is:
(1) surface cleaning: first Fresh leaf of aloe clear water wash clean, described clear water can not use the tap water containing bleach, because bleach can cause aloe liquid variable color, go bad;
(2) ultraviolet irradiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 6min;
(3) remove leaf skin: in sterilizing room, remove Folium Aloe skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum;
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 20000, time 3 minutes); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice;
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product;
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, inoculum concentration 4%, fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Aloe gel fermented product;
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, inoculum concentration 4%, fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Folium Aloe fermented product;
Described Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, the preparation method of Lignum cinnamomi camphorae leaf extract is: get pulverous Herba Portulacae respectively, Folium Kaki, orange peel, scutellariae,radix, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Trichotoma matsutake, Radix Ginseng, Radix Astragali root, Rhodiola rosea L. root, Testa oryzae, Flos Jasmini Sambac, Semen Persicae, Lignum cinnamomi camphorae leaf, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, namely obtaining Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, tricholoma matsutake, Radix Ginseng extract, Radix Astragali root extract, Rhodiola rosea L. root extract, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract respectively.
3. the eye cream containing Cordyceps militaris (L.) Link. composition as claimed in claim 1 or 2, it is characterized in that, described alkali ion water is by carrying out electrolysis generation by pure water.
4. the eye cream containing Cordyceps militaris (L.) Link. composition as claimed in claim 3, it is characterized in that, described alkali ion water index is pH:9.80-13.80, current potential: >=-120mV.
5. the preparation method of the eye cream containing Cordyceps militaris (L.) Link. composition as claimed in claim 4, is characterized in that:
Step one, component A added the first blending tank and stir and be warming up to 80-90 DEG C and keep 20 minutes;
Step 2, B1 and B2 component raw material added the second blending tank and stir intensification 80 DEG C and keep 20 minutes;
Step 3, the first blending tank Raw suction homogenizing tank to be stirred, then the raw material in the second blending tank is added in homogenizing tank;
Step 4, emulsifying, after 5 minutes, stir and are cooled to 60 DEG C;
Step 5, to add component C raw material stirring even, continues to be cooled to 45 DEG C;
Step 6, add after D component raw material stirs and be cooled to 35-38 DEG C, ageing 24 hours, fill, subpackage is also packed, and is finished product.
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| CN109481327A (en) * | 2018-12-04 | 2019-03-19 | 珠海依赛思生物科技有限公司 | A kind of multiple-effect maintenance peptide essence composition and preparation method thereof |
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