CN104367540A - Facial mask lotion containing cordyceps militaris ingredients and preparation method thereof - Google Patents
Facial mask lotion containing cordyceps militaris ingredients and preparation method thereof Download PDFInfo
- Publication number
- CN104367540A CN104367540A CN201410686582.3A CN201410686582A CN104367540A CN 104367540 A CN104367540 A CN 104367540A CN 201410686582 A CN201410686582 A CN 201410686582A CN 104367540 A CN104367540 A CN 104367540A
- Authority
- CN
- China
- Prior art keywords
- extract
- aloe
- component
- fermentation
- fermented product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a facial mask lotion containing cordyceps militaris ingredients. The facial mask lotion contains the following 22 active ingredients: fermented aloes, hydrolyzed oat proteins, a cordyceps extract, hydrolyzed royal jellies, a purslane extract, a persimmon leaf extract, an orange peel extract, a scutellaria baicalensis root extract, a phellinus linteus extract, a white willow bark extract, a vanilla extract, a Lengxiang rose extract, a mung bean extract, a green tea extract, a star anise extract, an astragalus membranaceus root extract, a ginseng root extract, a tricholoma matsutake extract, a rice bran extract, a jasmine extract, a peach seed extract and a cinnamomum camphora leaf extract. The invention also provides a preparation method of the facial mask lotion containing cordyceps militaris ingredients. The facial mask lotion prepared by adopting the method has the characteristics of safety, no toxicity and wrinkle resistance and is lower in production cost.
Description
Technical field
The present invention relates to cosmetic field, particularly relate to a kind of facial mask liquid containing Cordyceps militaris (L.) Link. composition and preparation method thereof.
Background technology
Cordyceps militaris (L.) Link. formal name used at school Cordyceps militaris (L.) Link. (
cordyceps militaris), it and Cordyceps of Qinghai Province belong to Eumycota Ascomycotina Cordyceps, therefore, are called belonging to together " brother " of Cordyceps by expert.The perfect stage of Cordyceps militaris (L.) Link. life cycle comprises mycelial growth and sporophore growth.The insect larvaes such as Lepidoptera, coleoptera, Diptera or pupa infect the spore of Paecilomyces varioti, and when environmental condition is suitable for, spore just sprouts formation mycelium, and mycelium draws the nutrition in worm (pupa) body as the material of its growth promoter and the energy.After mycelia decomposes the various tissue in worm (pupa) body and organ, hyphostoma development transfers reproductive growth to by nourishing and growing, mycelium twist together gradually be differentiated to form sclerotium and pass that the external top becoming crocus or Chinese red is slightly expanded, in bar-shaped Stroma (sporophore), the many heads from polypide of Stroma, chest, caudad etc. stretch out.
Wild Cordyceps militaris (L.) Link. is similar to traditional Cordyceps, very rare at occurring in nature.Most research is thought, Cordyceps militaris (L.) Link. is close with natural cordyceps on active ingredient, and its medicinal and edibility can match in excellence or beauty with natural Cordyceps.The Cordyceps militaris (L.) Link. cellulose content of factorial praluction is higher than natural cordyceps, and the content of its protein, aminoacid, vitamin and trace element has has all met or exceeded the level of natural cordyceps.Plant in Cordyceps reported more than 350, Cordyceps militaris (L.) Link. is the rare Cordyceps that can form a large amount of cordycepin.Because with Cordyceps sibship recently, effective ingredient is quite even higher, and can artificial culture, Cordyceps militaris (L.) Link. becomes the Ideal Substitute of natural cordyceps.Riched Cordyceps Militaris is containing various bioactivators, as Cordyceps polysaccharide, cordycepin, cordycepic acid, SOD enzyme, adenosine, polypeptide/aminoacid, mannitol etc., with the cosmetics that Cordyceps militaris (L.) Link. extract is made for raw material, then there is antioxidation, defying age and the effect such as nutrition, check melanin synthesis is provided, extra-nutrition can be produced to human skin, improve coarse, effect such as enhancing elasticity, moisturizing whitening etc.
To increase due to the age and the various factors such as seasonal variations, ultraviolet radiation, smoking, operating pressure are large, sitting before computer, television set, contact radioactive substance causes the skin problem of people, as skin corium subside, moisture-retaining capacity declines, xerosis cutis is aging.Therefore, cosmetics have become the requisite articles for daily use of one of people.Do not stimulate skin due to easy to use, safe, can frequently use, facial mask liquid has more and more been active status in Cosmetic Market.Facial mask liquid be clean, nurse, the cosmetics of nutrition skin of face, at present, commercially available facial mask liquid kind is a lot, but wherein great majority are all with the addition of some chemical compositions inside, consumer is after life-time service similar products, may there is anaphylaxis in skin, cause corse sweat pore, produce dependency, even bring out Other diseases.Therefore, need exploitation badly a kind of containing large number of biological active component, existing good result of use, the healthy Novel facial mask liquid of consumer can not be damaged again.
Summary of the invention
For the problems referred to above, the invention provides a kind of facial mask liquid containing Cordyceps militaris (L.) Link. composition and preparation method thereof, there is safety non-toxic, crease-resistant feature, and production cost is lower.
For realizing above-mentioned purpose of the present invention, the invention provides a kind of facial mask liquid containing Cordyceps militaris (L.) Link. composition, formulated by weight by following A, B1, B2, C, D, E and F component raw material.
Component A: pure water 10-25 part, card ripple U-20 1.85-4 part.
B1 component: pure water 13-17 part, hydrolysis avenin 1.5-3.8 part, xylitol 1.1-2.75 part, Trenaxmine 0.5-1.3 part.
B2 component: glycerol 1.5-2.5 part, xanthan gum 0.04-0.07 part.
Component C: butanediol 2-3.75 part, Nipagin ester (PHENONIP) 0.2-0.4 part, Fructus Anisi Stellati extract 0.25-0.5 part.
D component: deionized water 30-50 part.
Component E: potassium hydroxide (10%) 1.5-5 part.
F component: adenosine triphosphate disodium salt 0.02-0.04 part, glycyrrhizic acid dipotassium 0.03-0.06 part, Cordyceps extract 1.85-3 part, hydrolysis Lac regis apis 0.3-0.65 part, Aloe fermented product 0.4-0.8 part, Herba portulacae extract 0.05-0.3 part, scutellariae,radix extract 0.05-0.3 part, Folium Kaki extract 0.1-0.2 part, orange peel extract 0.01-0.05 part, phellinus linteus extract 0.01-0.03 part, Salix Babylonica L.P.E 0.01-0.03 part, vanilla extract 0.01-0.05 part, Lengxiang rose flower extract 0.01-0.05 part, Semen phaseoli radiati extract 0.01-0.04 part, green tea extract 0.01-0.02 part, Radix Astragali root extract 0.01-0.02 part, Radix Ginseng extract 0.01-0.02 part, tricholoma matsutake 0.01-0.05 part, rice bran extract 0.01-0.03 part, Flos Jasmini Sambac extract 0.01-0.05 part, Semen Persicae extract 0.01-0.02 part, Lignum cinnamomi camphorae leaf extract 0.01-0.03 part.
In described F component, the preparation method of Cordyceps extract is as follows.
(1) liquid submerged fermentation mode is adopted to obtain cordyceps mycelium.
The technique of Cordyceps militaris (L.) Link. deep fermentation is as follows: mycelia fermentation is basis set to be become: 20g/L sucrose+5g/L peptone+0.5g/L MgSO
47H
2o+1g/L KH
2pO
4+ 5g/L bacillus cereus tropina; Adopting two-stage control pH strategy, control ph constant a certain numerical value between 6.0-7.0 when namely fermentation starts, is a certain numerical value between 5.0-6.0 again by pH value constant control after inoculation Cordyceps militaris strain 40h-60h.Ventilation in fermentation tank controls between 1:0.3-l:0.6 that (unit is m
3/ m
3/ min), fermentation tank mixing speed controls at 120-200r/min.
(2) supersound extraction of mycelium fermentation broth.
Get the above-mentioned cordyceps mycelium fermentation liquid 100-200ml obtained through submerged fermentation, ultrasonic (59Hz) processes 15-45min, fermentation liquor collected after centrifugation centrifuged supernatant after supersound process, gained primary deposition thing adds 30-100ml alkali ion water again and carries out twice ultrasonic process, is total to supersound process three times successively; Merge three centrifuged supernatant, and with Rotary Evaporators (30 DEG C-50 DEG C, revolution 60rpm) concentrated solution of concentrated 20-30ml, add the dehydrated alcohol of concentrated solution 3 times amount, under 4 DEG C of conditions, precipitate 8-12h, incline and supernatant, precipitation absolute ethanol washing, drying, obtain polysaccharide crude, be Cordyceps extract.
Described alkali ion water is by carrying out electrolysis generation by pure water.
Described alkali ion water index is pH:9.80-13.80, current potential: >=-120mV.
In described F component, the preparation method of Aloe fermented product is.
(1) surface cleaning: Fresh leaf of aloe clear water wash clean, described clear water can not use the tap water containing bleach, because bleach can cause aloe liquid variable color, go bad.
(2) ultraviolet radiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 5-30min.
(3) remove leaf skin: in sterilizing room, remove Fresh leaf of aloe leaf skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum.
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 10000-30000, time 0.2-5 minute); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice.
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product.
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10%(percent by volume of bacterium liquid).Fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Aloe gel fermented product.
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10%(percent by volume of bacterium liquid).Fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Folium Aloe fermented product.
Described Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, the preparation method of Lignum cinnamomi camphorae leaf extract is: get pulverous Herba Portulacae respectively, Folium Kaki, orange peel, scutellariae,radix, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Fructus Anisi Stellati extract, Radix Astragali root, Radix Ginseng, Trichotoma matsutake, Testa oryzae, Flos Jasmini Sambac, Semen Persicae, Lignum cinnamomi camphorae leaf, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, being Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract.
The present invention also provides a kind of preparation method of the facial mask liquid containing Cordyceps militaris (L.) Link. composition, comprises the following steps.
Step one, component A raw material is added blending tank by after formula metering, stir (revolution 30-100rpm) and be uniformly dispersed, be warming up to 80-90 DEG C, stirring and dissolving.
Step 2, add B1 and B2 component raw material stirring and dissolving, and after continuing stirring and dissolving in 80-90 DEG C, be cooled to 40-45 DEG C.
Step 3, by mix homogeneously after the 40 DEG C of preheatings of component C raw material, add emulsifying pot, stir, add D component raw material and stir.
Step 4, to add component E raw material stirring even, and adjust ph is to 5.5-6.0.
Step 5, add F component raw material and stir, vacuum outgas 10-20 minute (vacuum is 0.09-0.10MPa).
Step 6, be cooled to 30-40 DEG C, ageing 24 hours, fill, subpackage is also packed, and is finished product.
Compared with prior art beneficial effect of the present invention.
The invention provides a kind of facial mask liquid containing Cordyceps militaris (L.) Link. composition and preparation method thereof, the described facial mask liquid containing Cordyceps militaris (L.) Link. composition contains Aloe fermented product, hydrolysis avenin, Cordyceps extracting solution, hydrolysis Lac regis apis, Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract, totally two ten two kinds of active component.Wherein Aloe fermented product has elimination scytitis and antibacterial action, and can significantly improve the anti-wrinkle effect of facial mask liquid, is especially applicable to middle and aged women and uses.Herba Portulacae contains abundant SL3 fatty acid and vitamin A sample material.SL3 fatty acid forms the necessary material of cell membrane; Vitamin A sample substance maintains epithelial tissue as the normal function of skin, cornea.Orange peel extract contains terpenoid substance, plays antibacterial and natural perfume material.Containing a large amount of tannic acid in Folium Kaki, can effectively kill various antibacterial and fungus, there is effect that is clean and skin care.Lac regis apis rich in proteins, the material such as aminoacid, vitamin, lipoid, certain herbaceous plants with big flowers acetic acid, trace element, folic acid, acetylcholine, nucleic acid, has the effects such as regulation of skin metabolism and antioxidation.Hydrolysis avenin is rich in hydrolyzed protein, plays instant convergence and level and smooth effect; Scutellariae,radix extract is rich in flavone compound, has the effects such as antibacterial and contraction.Flos Jasmini Sambac extract has antioxidation and bacteria resistance function.In addition, adopt liquid submerged fermentation mode to obtain cordyceps mycelium in the present invention, have that cultivation cycle is short, active substance is easy to the advantages such as extraction, the active matter content of a lot of medicinal fungi liquid fermentation is far above the active matter content of tame medicinal fungi; Add the tropina of bacillus cereus in mycelium fermentation broth, both improved the output of cordyceps mycelium, additionally reduce peptone consumption, greatly save cost of material.
Detailed description of the invention
The present invention is further described below in conjunction with specific embodiment.
The invention provides a kind of facial mask liquid containing Cordyceps militaris (L.) Link. composition, formulated by weight by following A, B1, B2, C, D, E and F component raw material.
Component A: pure water 17 parts, card ripple U-20 3.75 parts.
B1 component: pure water 15 parts, hydrolysis avenin 2 parts, xylitol 1.5 parts, Trenaxmine 1 part.
B2 component: glycerol 1.9 parts, xanthan gum 0.06 part.
Component C: butanediol 2.5 parts, Nipagin ester (PHENONIP) 0.3 part, Fructus Anisi Stellati extract 0.35 part.
D component: deionized water 43 parts.
Component E: potassium hydroxide (10%) 3.5 part.
F component: adenosine triphosphate disodium salt 0.03 part, glycyrrhizic acid dipotassium 0.04 part, Cordyceps extract 2.4 parts, hydrolysis Lac regis apis 0.5 part, Aloe fermented product 0.5 part, Herba portulacae extract 0.1 part, scutellariae,radix extract 0.15 part, Folium Kaki extract 0.1 part, orange peel extract 0.02 part, phellinus linteus extract 0.01 part, Salix Babylonica L.P.E 0.02 part, vanilla extract 0.02 part, Lengxiang rose flower extract 0.01 part, Semen phaseoli radiati extract 0.04 part, green tea extract 0.015 part, Radix Astragali root extract 0.01 part, Radix Ginseng extract 0.01 part, tricholoma matsutake 0.04 part, rice bran extract 0.01 part, Flos Jasmini Sambac extract 0.05 part, 0.01 part, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract 0.03 part.
In described F component, the preparation method of Cordyceps extract is as follows.
(1) liquid submerged fermentation mode is adopted to obtain cordyceps mycelium.
Wild cordyceps militaris is at the Pupa bombycis tumor growth containing a large amount of insect protein, and the source of cultivating protein in the submerged fermentation culture medium of cordyceps mycelium is at present mainly peptone, has certain difference with pupa albumen.The present invention adopt add with Pupa bombycis insect protein closer to bacillus cereus tropina, make cordyceps mycelium fermentation production rate obtain improve.Mycelia fermentation is basis set to be become: 20g/L sucrose+5g/L peptone+0.5g/L MgSO
47H
2o+1g/L KH
2pO
4+ 5g/L bacillus cereus tropina; Adopt two-stage control pH strategy, when namely fermentation starts, control ph is constant in 6.2, inoculation Cordyceps militaris strain (this liquid spawn by Cordyceps militaris (L.) Link. (
cordyceps militaris) fermentation) and afterwards 50h be 5.6 by pH value constant control again.Ventilation in fermentation tank controls between 1:0.4 that (unit is m
3/ m
3/ min), fermentation tank mixing speed controls at 180r/min.
(2) supersound extraction of mycelium fermentation broth.
Get the above-mentioned cordyceps mycelium fermentation liquid 100ml obtained through submerged fermentation, ultrasonic (59Hz) processes 30min, fermentation liquor collected after centrifugation centrifuged supernatant after supersound process, gained precipitate adds 100ml alkali ion water again and carries out twice ultrasonic extraction, is total to supersound extraction three times successively.Merge three centrifuged supernatant and use Rotary Evaporators (40 DEG C, revolution 60rpm) concentrated solution of concentrated 30ml, add the dehydrated alcohol of concentrated solution 3 times amount, 10h is precipitated under 4 DEG C of conditions, incline and supernatant and obtain precipitate, this precipitate absolute ethanol washing, drying, obtain polysaccharide crude, be Cordyceps extract.
Described alkali ion water is by carrying out electrolysis generation by pure water; Described pure water is after electrolysis, and enrichment hydroxide ion in water near negative electrode, in alkalescence, obtains alkali ion water.
The alkali ion water index that described alkali ion water outlet produces is pH:9.80-13.80, current potential: >=-120mv.
With distilled water extraction cordyceps mycelium effective ingredient, extraction efficiency is 16.5%, and the extraction efficiency of alkali ion water is 23.9%.As can be seen here, described alkali ion water extraction cordyceps mycelium effective ingredient has not only increased substantially extraction efficiency, also avoid the pollution because using chemical solvent to bring.
In described F component, the preparation method of Aloe fermented product is.
(1) surface cleaning: first Fresh leaf of aloe clear water wash clean, described clear water can not use the tap water containing bleach, because bleach can cause aloe liquid variable color, go bad.
(2) ultraviolet irradiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 6min.
(3) remove leaf skin: in sterilizing room, remove Folium Aloe skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum.
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 20000, time 3 minutes); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice.
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product.
1. be, with aloe gel juice culture medium, with Hansenula anomala (lot number: AS2.297, Bei Nuo bio tech ltd, Shanghai) for inoculation strain, inoculum concentration 4%(percent by volume).Fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Aloe gel fermented product.
2., with Folium Aloe natural juice for culture medium, with Hansenula anomala (lot number: AS2.297, Bei Nuo bio tech ltd, Shanghai) for inoculation strain, inoculum concentration 4%(percent by volume).Fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Folium Aloe fermented product.
Prior art commonly uses Aloe original juice, and the present invention adopts Aloe fermented product, and this Aloe fermented product has following benefit compared with the Aloe original juice not through fermenting: composition is more various and molecule is less, thus is more easily absorbed by the skin.
Described Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, the preparation method of Lignum cinnamomi camphorae leaf extract is: get pulverous Herba Portulacae respectively, Folium Kaki, orange peel, scutellariae,radix, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Fructus Anisi Stellati, Radix Astragali root, Radix Ginseng, Trichotoma matsutake, Testa oryzae, Flos Jasmini Sambac, Semen Persicae, Lignum cinnamomi camphorae leaf, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, being Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract.
The present invention also provides a kind of preparation method of the facial mask liquid containing Cordyceps militaris (L.) Link. composition, comprises the following steps.
Step one, component A raw material is added blending tank by after formula metering, stir (revolution 30-100rpm) and be uniformly dispersed, be warming up to 80 DEG C, stirring and dissolving.
Step 2, add B1 and B2 component raw material stirring and dissolving, and after continuing stirring and dissolving in 80 DEG C, be cooled to 40 DEG C.
Step 3, by mix homogeneously after the 40 DEG C of preheatings of component C raw material, add emulsifying pot, stir, add D component raw material and stir.
Step 4, to add component E raw material stirring even, adjust ph to 6.0.
Step 5, add F component raw material and stir, vacuum outgas 10 minutes (vacuum is 0.09-0.10MPa).
Step 6, be cooled to 30 DEG C, ageing 24 hours, fill, subpackage is also packed, and is finished product.
The efficacy assessments of the present embodiment formula composition and preparation method thereof gained facial mask liquid product.
1, practical effect evaluation: participating in facial mask liquid effect evaluation and test interviewee number is 30 people, the present embodiment of applying ointment or plaster, containing the facial mask liquid 3 times weekly of Cordyceps militaris (L.) Link. composition, is for 10 times a course for the treatment of.Use the present embodiment containing after facial mask liquid one course for the treatment of of Cordyceps militaris (L.) Link. composition, objective evaluation is made to this facial mask liquid moisturizing, moisturizing, elite trap, elite volatility, facial mask liquid abnormal smells from the patient, often kind of effect judgment criteria is divided into third gear, wherein moisturizing: first grade is that moistening effect is very good, second gear is that moistening effect is general, and third gear is that moistening effect is bad; Moisturizing: first grade for moisturizing effect clearly, second gear is that moisturizing effect is general, and third gear is that moisturizing effect is bad; Elite trap: first grade is easy to absorb for elite, and second gear is that elite assimilation effect is general, and third gear is that elite assimilation effect is bad; Elite volatility: first grade is still have a large amount of elite after using, second gear is elite amount part minimizing after use, and third gear obviously reduces for using rear elite amount; Abnormal smells from the patient: first grade is natural comfortable, second gear is that chemical reagent is obvious, and third gear is not for having peculiar taste.Concrete evaluation result is in table 1.
Table 1: the present invention evaluates containing the facial mask liquid result of use of Cordyceps militaris (L.) Link. composition.
| First grade | Second gear | Third gear | |
| Moisturizing | 20 | 10 | 0 |
| Moisturizing | 19 | 11 | 0 |
| Elite trap | 25 | 5 | 0 |
| Elite volatility | 0 | 4 | 26 |
| Abnormal smells from the patient | 6 | 0 | 24 |
As shown in Table 1, the present invention has the effect of good moisturizing, moisturizing, nutrition, convergence containing the facial mask liquid of Cordyceps militaris (L.) Link. composition.
2, moisture-keeping efficacy evaluation: moisture-keeping efficacy evaluation adopts external weight method, according to the difference of cosmetic composition moisture absorption, performance of keeping humidity, different wetting agent molecules is different to the active force of hydrone, absorbs moisture different with keeping the ability of moisture.Hygroscopic effect power is large, strong to water molecules power, and absorb and keep the amount of moisture also larger, closure is good, also lacking of moisture loss.Utilize adhesive tape to imitate the biomaterial such as horny layer, epidermis, cosmetics smeared by adhesive tape, the application feature of simulation pragmatize cosmetic.Under the condition of constant temperature and humidity after certain hour (4 hours), weigh sample and place the of poor quality of front and back, obtain the loss of sample size, the effect of moisturizing ingredient moisturizing can be calculated.
With the present embodiment facial mask liquid liquid for sample is directly measured, determine that the moisturizing rate of this facial mask liquid is about 51.8% by experiment.
3, senile-resistant efficacy evaluation: senile-resistant efficacy evaluation adopts in-vitro evaluation method, and working sample is to the Scavenging activity of free radical.Comprise three indexs, be respectively: remove the mensuration of DPPH ability, remove the mensuration of superoxide anion ability and the mensuration of scavenging hydroxyl ability.
In the mensuration removing DPPH ability, the present embodiment is 22.99%-48.05% containing the clearance rate of the facial mask liquid of Cordyceps militaris (L.) Link. composition; In the mensuration removing superoxide anion ability, the present embodiment is 22.06%-47.1% containing the facial mask liquid of Cordyceps militaris (L.) Link. composition to the suppression ratio of superoxide anion; In the mensuration of scavenging hydroxyl ability, the present embodiment is 15.98%-26.15% containing the facial mask liquid of Cordyceps militaris (L.) Link. composition to the clearance rate of hydroxy radical.
4, pH value measures: take facial mask liquid sample 1g(and be accurate to 0.1g), adding distil water 10g, is heated to 40 DEG C, and is constantly stirred to evenly, measures after being cooled to room temperature with pH meter.
Mensuration the present embodiment is 5.7-7.0 containing the pH value of the facial mask liquid of Cordyceps militaris (L.) Link. composition.
5, measuring drying time: the tackness that can reflect facial mask liquid drying time, is one of important indicator weighing facial mask liquid quality.Facial mask liquid is too short for drying time, and effective ingredient is not easily absorbed by the skin, and easily produces strong pulling force to skin and caused discomfort; Drying time is long, uses inconvenience, and skin dehydration is more, and the assimilation effect of effective ingredient is not good enough yet.Particularly, take 0.2g facial mask liquid sample, uniform application, on clean microscope slide (25.4mm × 76.2mm), makes facial mask liquid sample tile whole slide surface; Then, at being placed in room temperature 20 ± 5 DEG C, recording drying time, be as the criterion with tack-free.
Mensuration the present embodiment is 15-30min containing the drying time of the facial mask liquid of Cordyceps militaris (L.) Link. composition.
6, Stability Determination.
(1) heat resistant test: be placed in 40 DEG C and deposit 24h, observe whether have layering, oil slick phenomenon, whether color and luster changes.After tested, the present embodiment finds the front no significant difference of itself and test containing the facial mask liquid visual observations of Cordyceps militaris (L.) Link. composition, and layering, oil slick phenomenon do not occur, and color and luster, also without significant change, shows that facial mask liquid product thermostability is better.
(2) low temperature resistant test: be placed in-5 DEG C and deposit 24h, observe whether have layering, oil slick phenomenon, whether color and luster changes.After tested, the present embodiment finds the front no significant difference of itself and test containing the facial mask liquid visual observations of Cordyceps militaris (L.) Link. composition, and layering, oil slick phenomenon do not occur, and color and luster, also without significant change, shows that facial mask liquid product tolerance to cold is better.
(3) thermal cycling test: be placed in 40 DEG C and deposit 24h, deposit 24h, then deposit 24h in-5 DEG C in room temperature, circulates 3 times successively, and observe whether have layering, oil slick and oil impregnate phenomenon, whether color and luster changes.After tested, the present embodiment finds the front no significant difference of itself and test containing the facial mask liquid visual observations of Cordyceps militaris (L.) Link. composition, and layering, oil slick phenomenon do not occur, and color and luster, also without significant change, shows that facial mask liquid product is cold-resistant better hot.
(4) whether dewatering ability: centrifugal 20min under being placed in 300 r/min, observe and have moisture to separate out.After tested, containing the facial mask liquid visual observations of Cordyceps militaris (L.) Link. composition, the present embodiment finds that its anhydrous assay goes out, lamination does not occur, shows that facial mask liquid product dewatering ability is better.
Claims (5)
1. containing a facial mask liquid for Cordyceps militaris (L.) Link. composition, formulated by following A, B1, B2, C, D, E and F component raw material:
Component A: pure water 10-25 part, card ripple U-20 1.85-4 part:
B1 component: pure water 13-17 part, hydrolysis avenin 1.5-3.8 part, xylitol 1.1-2.75 part, Trenaxmine 0.5-1.3 part:
B2 component: glycerol 1.5-2.5 part, xanthan gum 0.04-0.07 part:
Component C: butanediol 2-3.75 part, Nipagin ester 0.2-0.4 part, Fructus Anisi Stellati extract 0.25-0.5 part:
D component: deionized water 30-50 part:
Component E: potassium hydroxide 1.5-5 part:
F component: adenosine triphosphate disodium salt 0.02-0.04 part, glycyrrhizic acid dipotassium 0.03-0.06 part, Cordyceps extract 1.85-3 part, hydrolysis Lac regis apis 0.3-0.65 part, Aloe fermented product 0.4-0.8 part, Herba portulacae extract 0.05-0.3 part, scutellariae,radix extract 0.05-0.3 part, Folium Kaki extract 0.1-0.2 part, orange peel extract 0.01-0.05 part, phellinus linteus extract 0.01-0.03 part, Salix Babylonica L.P.E 0.01-0.03 part, vanilla extract 0.01-0.05 part, Lengxiang rose flower extract 0.01-0.05 part, Semen phaseoli radiati extract 0.01-0.04 part, green tea extract 0.01-0.02 part, Radix Astragali root extract 0.01-0.02 part, Radix Ginseng extract 0.01-0.02 part, tricholoma matsutake 0.01-0.05 part, rice bran extract 0.01-0.03 part, Flos Jasmini Sambac extract 0.01-0.05 part, Semen Persicae extract 0.01-0.02 part, Lignum cinnamomi camphorae leaf extract 0.01-0.03 part,
In described D component, the preparation method of Cordyceps extract is:
(1) liquid submerged fermentation mode is adopted to obtain cordyceps mycelium:
The technique of Cordyceps militaris (L.) Link. deep fermentation is as follows: mycelia fermentation is basis set to be become: 20g/L sucrose+5g/L peptone+0.5g/L MgSO
47H
2o+1g/L KH
2pO
4+ 5g/L bacillus cereus tropina; Adopting two-stage control pH strategy, control ph constant a certain numerical value between 6.0-7.0 when namely fermentation starts, is a certain numerical value between 5.0-6.0 again by pH value constant control after inoculation Cordyceps militaris strain 40h-60h; Ventilation in fermentation tank controls between 1:0.3-l:0.6 that (unit is m
3/ m
3/ min), fermentation tank mixing speed controls at 120-200r/min;
(2) supersound extraction of mycelium fermentation broth: get the above-mentioned cordyceps mycelium fermentation liquid 100-200ml obtained through submerged fermentation, ultrasonic (59Hz) processes 15-45min, fermentation liquor collected after centrifugation centrifuged supernatant after supersound process, gained primary deposition thing adds 30-100ml alkali ion water again and carries out twice ultrasonic process, is total to supersound process three times successively; Merge three centrifuged supernatant, and with Rotary Evaporators (30 DEG C-50 DEG C, revolution 60rpm) concentrated solution of concentrated 20-30ml, add the dehydrated alcohol of concentrated solution 3 times amount, under 4 DEG C of conditions, precipitate 8-12h, incline and supernatant, precipitation absolute ethanol washing, drying, obtain polysaccharide crude, be Cordyceps extract;
In described F component, the preparation method of Aloe fermented product is:
(1) surface cleaning: Fresh leaf of aloe clear water wash clean;
(2) ultraviolet radiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 5-30min;
(3) remove leaf skin: in sterilizing room, remove Fresh leaf of aloe leaf skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum;
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 10000-30000, time 0.2-5 minute); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice;
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product;
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10% of bacterium liquid, fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Aloe gel fermented product;
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10% of bacterium liquid, fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Folium Aloe fermented product;
Described Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, the preparation method of Lignum cinnamomi camphorae leaf extract is: get pulverous Herba Portulacae respectively, Folium Kaki, orange peel, scutellariae,radix, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Fructus Anisi Stellati extract, Radix Astragali root, Radix Ginseng, Trichotoma matsutake, Testa oryzae, Flos Jasmini Sambac, Semen Persicae, Lignum cinnamomi camphorae leaf, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, being Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract.
2. the facial mask liquid containing Cordyceps militaris (L.) Link. composition as claimed in claim 1, is characterized in that, formulated by following A, B1, B2, C, D, E and F component raw material:
Component A: pure water 17 parts, card ripple U-20 3.75 parts;
B1 component: pure water 15 parts, hydrolysis avenin 2 parts, xylitol 1.5 parts, Trenaxmine 1 part;
B2 component: glycerol 1.9 parts, xanthan gum 0.06 part;
Component C: butanediol 2.5 parts, Nipagin ester 0.3 part, Fructus Anisi Stellati extract 0.35 part;
D component: deionized water 43 parts;
Component E: potassium hydroxide 3.5 parts;
F component: adenosine triphosphate disodium salt 0.03 part, glycyrrhizic acid dipotassium 0.04 part, Cordyceps extract 2.4 parts, hydrolysis Lac regis apis 0.5 part, Aloe fermented product 0.5 part, Herba portulacae extract 0.1 part, scutellariae,radix extract 0.15 part, Folium Kaki extract 0.1 part, orange peel extract 0.02 part, phellinus linteus extract 0.01 part, Salix Babylonica L.P.E 0.02 part, vanilla extract 0.02 part, Lengxiang rose flower extract 0.01 part, Semen phaseoli radiati extract 0.04 part, green tea extract 0.015 part, Radix Astragali root extract 0.01 part, Radix Ginseng extract 0.01 part, tricholoma matsutake 0.04 part, rice bran extract 0.01 part, Flos Jasmini Sambac extract 0.05 part, 0.01 part, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract 0.03 part,
In described F component, the preparation method of Cordyceps extract is:
(1) liquid submerged fermentation mode is adopted to obtain cordyceps mycelium:
Mycelia fermentation is basis set to be become: 20g/L sucrose+5g/L peptone+0.5g/L MgSO
47H
2o+1g/L KH
2pO
4+ 5g/L bacillus cereus tropina; Adopt two-stage control pH strategy, when namely fermentation starts, control ph is constant in 6.2, and after inoculation Cordyceps militaris strain, pH value constant control is 5.6 by 50h again; Ventilation in fermentation tank controls between 1:0.4 that (unit is m
3/ m
3/ min), fermentation tank mixing speed controls at 180r/min;
(2) supersound extraction of mycelium fermentation broth: get the above-mentioned cordyceps mycelium fermentation liquid 100ml obtained through submerged fermentation, ultrasonic (59Hz) processes 30min, fermentation liquor collected after centrifugation centrifuged supernatant after supersound process, gained precipitate adds 100ml alkali ion water again and carries out twice ultrasonic extraction, is total to supersound extraction three times successively; Merge three centrifuged supernatant and use Rotary Evaporators (40 DEG C, revolution 60rpm) concentrated solution of concentrated 30ml, add the dehydrated alcohol of concentrated solution 3 times amount, 10h is precipitated under 4 DEG C of conditions, incline and supernatant and obtain precipitate, this precipitate absolute ethanol washing, drying, obtain polysaccharide crude, be Cordyceps extract;
In described F component, the preparation method of Aloe fermented product is:
(1) surface cleaning: Fresh leaf of aloe clear water wash clean;
(2) ultraviolet irradiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 6min;
(3) remove leaf skin: in sterilizing room, remove Folium Aloe skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum;
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 20000, time 3 minutes); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice;
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product;
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, inoculum concentration 4%, fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Aloe gel fermented product;
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, inoculum concentration 4%, fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Folium Aloe fermented product;
Described Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, the preparation method of Lignum cinnamomi camphorae leaf extract is: get pulverous Herba Portulacae respectively, Folium Kaki, orange peel, scutellariae,radix, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Fructus Anisi Stellati extract, Radix Astragali root, Radix Ginseng, Trichotoma matsutake, Testa oryzae, Flos Jasmini Sambac, Semen Persicae, Lignum cinnamomi camphorae leaf, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, being Herba portulacae extract, Folium Kaki extract, orange peel extract, scutellariae,radix extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, rice bran extract, Flos Jasmini Sambac extract, Semen Persicae extract, Lignum cinnamomi camphorae leaf extract.
3. the facial mask liquid containing Cordyceps militaris (L.) Link. composition as claimed in claim 1 or 2, it is characterized in that, described alkali ion water is by carrying out electrolysis generation by pure water.
4. the facial mask liquid containing Cordyceps militaris (L.) Link. composition as claimed in claim 3, it is characterized in that, described alkali ion water index is pH:9.80-13.80, current potential: >=-120mV.
5. the preparation method of the facial mask liquid containing Cordyceps militaris (L.) Link. composition as claimed in claim 4, is characterized in that:
Step one, component A raw material is added blending tank by after formula metering, stir (revolution 30-100rpm) and be uniformly dispersed, be warming up to 80-90 DEG C, stirring and dissolving;
Step 2, add B1 and B2 component raw material stirring and dissolving, and after continuing stirring and dissolving in 80-90 DEG C, be cooled to 40-45 DEG C;
Step 3, by mix homogeneously after the 40 DEG C of preheatings of component C raw material, add emulsifying pot, stir, add D component raw material and stir;
Step 4, to add component E raw material stirring even, and adjust ph is to 5.5-6.0;
Step 5, add F component raw material and stir, vacuum outgas 10-20 minute;
Step 6, be cooled to 30-40 DEG C, ageing 24 hours, fill, subpackage is also packed, and is finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410686582.3A CN104367540A (en) | 2014-11-25 | 2014-11-25 | Facial mask lotion containing cordyceps militaris ingredients and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410686582.3A CN104367540A (en) | 2014-11-25 | 2014-11-25 | Facial mask lotion containing cordyceps militaris ingredients and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104367540A true CN104367540A (en) | 2015-02-25 |
Family
ID=52546905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410686582.3A Pending CN104367540A (en) | 2014-11-25 | 2014-11-25 | Facial mask lotion containing cordyceps militaris ingredients and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104367540A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109044946A (en) * | 2018-09-27 | 2018-12-21 | 张言聚 | A kind of whitening and moisturizing facial mask liquid and its preparation process |
| CN109077954A (en) * | 2018-08-06 | 2018-12-25 | 佗鹊堂国投生物科技股份有限公司 | Cordyceps sinensis facial mask |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120014694A (en) * | 2010-08-10 | 2012-02-20 | 박미연 | Method for manufacturing a skin-lightening material using a culture fluid of cordyceps militaris |
| CN103202788A (en) * | 2013-04-25 | 2013-07-17 | 南宁骄阳医药科技有限公司 | Spot-fading cream taking persimmon leaf extract as functional raw material |
| CN103462858A (en) * | 2013-09-26 | 2013-12-25 | 北京美丽铺化妆品有限公司 | Oat paste having effects of cleaning and skin care, mask and their preparation methods |
| CN104152514A (en) * | 2014-07-24 | 2014-11-19 | 北京翰若生物科技有限公司 | Origin fermentation method and origin fermentation product prepared by same |
-
2014
- 2014-11-25 CN CN201410686582.3A patent/CN104367540A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120014694A (en) * | 2010-08-10 | 2012-02-20 | 박미연 | Method for manufacturing a skin-lightening material using a culture fluid of cordyceps militaris |
| CN103202788A (en) * | 2013-04-25 | 2013-07-17 | 南宁骄阳医药科技有限公司 | Spot-fading cream taking persimmon leaf extract as functional raw material |
| CN103462858A (en) * | 2013-09-26 | 2013-12-25 | 北京美丽铺化妆品有限公司 | Oat paste having effects of cleaning and skin care, mask and their preparation methods |
| CN104152514A (en) * | 2014-07-24 | 2014-11-19 | 北京翰若生物科技有限公司 | Origin fermentation method and origin fermentation product prepared by same |
Non-Patent Citations (3)
| Title |
|---|
| 中华人民共和国卫生部: "《化妆品卫生规范》", 31 January 2007 * |
| 赵润等: "冬虫夏草菌丝体液体培养基的优化", 《天津师范大学学报（自然科学版）》 * |
| 陈晋安等: "蛹虫草液体发酵条件的研究", 《集美大学学报(自然科学版)》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109077954A (en) * | 2018-08-06 | 2018-12-25 | 佗鹊堂国投生物科技股份有限公司 | Cordyceps sinensis facial mask |
| CN109044946A (en) * | 2018-09-27 | 2018-12-21 | 张言聚 | A kind of whitening and moisturizing facial mask liquid and its preparation process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104306323A (en) | Cordyceps-militaris-ingredient contained eye cream and preparing method of cordyceps-militaris-ingredient contained eye cream | |
| CN104473828A (en) | Dragon fruit stem moisturizing lotion and preparation method thereof | |
| CN107802543B (en) | Roxburgh rose element skin-refreshing biological fiber mask and preparation method thereof | |
| KR20110092721A (en) | The composite make natural korean herb cosmetics from genseng saponin rg1 and polia coccos ferment | |
| CN104434760A (en) | Facial cream containing ingredient of cordyceps militaris and preparation method thereof | |
| CN101961298B (en) | Moisturizing composition | |
| CN108815072A (en) | A kind of plant composition fermentation magma and its preparation method and application | |
| CN104352422A (en) | Eye cream containing cordyceps militaris component and preparation method of eye cream | |
| CN104367544A (en) | Primary liquid containing cordyceps militaris ingredients and preparation method thereof | |
| CN104352426A (en) | Facial mask fluid containing cordyceps militaris ingredient and preparation method thereof | |
| CN104306322A (en) | Cordyceps-militaris-ingredient contained eye cream and preparing method of cordyceps-militaris-ingredient contained eye cream | |
| CN104352424B (en) | Cordyceps militaris ingredient containing mask and preparation method thereof | |
| CN108721192A (en) | A kind of extracted form natural plant essence facial mask liquid and its extraction process | |
| CN112137919B (en) | Application of dictyophora indusiata egg extract in preparation of external beauty and skin care products and external beauty and skin care products based on same | |
| CN104367540A (en) | Facial mask lotion containing cordyceps militaris ingredients and preparation method thereof | |
| CN104367534A (en) | Essence containing cordyceps militaris ingredients and preparation method thereof | |
| CN104434759A (en) | Eye cream containing ingredient of cordyceps militaris and preparation method thereof | |
| CN111821230A (en) | Anti-allergy repairing cosmetic composition and application thereof | |
| CN104382836A (en) | Stoste containing cordyceps militaris and preparation method of stoste | |
| CN104323979A (en) | Eye cream containing cordyceps militaris ingredient and preparation method of eye cream | |
| CN104367539A (en) | Conditioning lotion containing cordyceps militaris ingredients and preparation method thereof | |
| CN103340808B (en) | A kind of preparation technology of pseudo-ginseng medicament night cream | |
| CN113413326B (en) | Rice wine lees polypeptide mask and preparation method and application thereof | |
| CN104367535A (en) | Essence containing cordyceps militaris ingredients and preparation method thereof | |
| CN104367536A (en) | Facial mask containing cordyceps militaris ingredients and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150225 |
|
| RJ01 | Rejection of invention patent application after publication |