CN104382836A - Stoste containing cordyceps militaris and preparation method of stoste - Google Patents
Stoste containing cordyceps militaris and preparation method of stoste Download PDFInfo
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Abstract
The invention relates to stoste containing cordyceps militaris. The stoste disclosed by the invention contains 21 active components including hydrolysed bee milk, aloe ferment, worm grass extract, purslane extract, scutellaria root extract, persimmon leaf extract, orange peel extract, phellinus igniarius extract, salicis extract, vanilla extract, Lengxiang rose extract, mung bean extract, green tea extract, illicium verum extract, astragalus membranaceus root extract, ginseng extract, tricholoma matsutake extract, lithospermum extract, chamomile extract, tree moss extract and honeysuckle extract. The stoste disclosed by the invention is safe and nontoxic. The invention further provides a preparation method of the stoste containing cordyceps militaris. The preparation method is simple; and furthermore, the production cost is effectively reduced.
Description
Technical field
The present invention relates to cosmetic field, particularly relate to a kind of stock solution containing Cordyceps militaris (L.) Link. composition and preparation method thereof.
Background technology
Cordyceps militaris (L.) Link. formal name used at school Cordyceps militaris (L.) Link. (
cordyceps militaris), it and Cordyceps of Qinghai Province belong to Eumycota Ascomycotina Cordyceps, therefore, are called belonging to together " brother " of Cordyceps by expert.The perfect stage of Cordyceps militaris (L.) Link. life cycle comprises mycelial growth and sporophore growth.The insect larvaes such as Lepidoptera, coleoptera, Diptera or pupa infect the spore of Paecilomyces varioti, and when environmental condition is suitable for, spore just sprouts formation mycelium, and mycelium draws the nutrition in worm (pupa) body as the material of its growth promoter and the energy.After mycelia decomposes the various tissue in worm (pupa) body and organ, hyphostoma development transfers reproductive growth to by nourishing and growing, mycelium twist together gradually be differentiated to form sclerotium and pass that the external top becoming crocus or Chinese red is slightly expanded, in bar-shaped Stroma (sporophore), the many heads from polypide of Stroma, chest, caudad etc. stretch out.
Wild Cordyceps militaris (L.) Link. is similar to traditional Cordyceps, very rare at occurring in nature.Most research is thought, Cordyceps militaris (L.) Link. is close with natural cordyceps on active ingredient, and its medicinal and edibility can match in excellence or beauty with natural Cordyceps.The Cordyceps militaris (L.) Link. cellulose content of factorial praluction is higher than natural cordyceps, and the content of its protein, aminoacid, vitamin and trace element has has all met or exceeded the level of natural cordyceps.Plant in Cordyceps reported more than 350, Cordyceps militaris (L.) Link. is the rare Cordyceps that can form a large amount of cordycepin.Because with Cordyceps sibship recently, effective ingredient is quite even higher, and can artificial culture, Cordyceps militaris (L.) Link. becomes the Ideal Substitute of natural cordyceps.Riched Cordyceps Militaris is containing various bioactivators, as Cordyceps polysaccharide, cordycepin, cordycepic acid, SOD enzyme, adenosine, polypeptide/aminoacid, mannitol etc., with the cosmetics that Cordyceps militaris (L.) Link. extract is made for raw material, then there is antioxidation, defying age and the effect such as nutrition, check melanin synthesis is provided, extra-nutrition can be produced to human skin, improve coarse, effect such as enhancing elasticity, moisturizing whitening etc.
To increase due to the age and the various factors such as seasonal variations, ultraviolet radiation, smoking, operating pressure are large, sitting before computer, television set, contact radioactive substance causes the skin problem of people, as skin corium subside, moisture-retaining capacity declines, xerosis cutis is aging.Therefore, cosmetics have become the requisite articles for daily use of one of people.Stock solution in cosmetic industry, refers to the high concentration skin care ingredient that interpolation is relatively single, can need, more direct to skin, safer, more potent maintenance for various skin, allows skin recover the cosmetics of optimum state at short notice.Active constituent content in stock solution is high, and energy quick-searching skin care demand, to the intensive potent maintenance of skin.At present, commercially available stock solution is divided into many types, but effect, function difference are larger.Therefore, need a kind of existing good result of use of exploitation badly, the healthy novel stock solution of consumer can not be damaged again.
Summary of the invention
For the problems referred to above, the invention provides a kind of stock solution containing Cordyceps militaris (L.) Link. composition and preparation method thereof, there is safety non-toxic, nourish crease-resistant feature, and production cost is lower.
For realizing above-mentioned purpose of the present invention, the invention provides a kind of stock solution containing Cordyceps militaris (L.) Link. composition, formulated by weight by following A, B, C and D component raw material.
Component A: pure water 30-45 part, glycyrrhizic acid dipotassium 0.75-3 part, trehalose 1.5-2.5 part, magnificent 1% solution in HA(micromolecule sea) 2.8-5 part, silk sliding natto gum 2-3.5 part.
B component: glycerol 1.9-2.85 part, xanthan gum 1-2.5 part, carboxymethyl R-glucan 0.1-0.3 part.
Component C: butanediol 1-2 part, 1,2 hexanediol 0.5-1.85 parts, Nipagin ester 0.15-0.4 part.
D component: hydrolysis Lac regis apis albumen 0.4-0.65 part, hEGF 0.02-0.04 part, acid fibroblast growth factor 0.15-0.5 part, metallothionein 0.015-0.03 part, Herba portulacae extract 0.1-0.4 part, Cordyceps extract 5-8.5 part, Aloe fermented product 0.1-0.5 part, scutellariae,radix extract 0.05-0.2 part, Folium Kaki extract 0.01-0.03 part, orange peel extract 0.01-0.05 part, phellinus linteus extract 0.03-0.06 part, Salix Babylonica L.P.E 0.01-0.03 part, vanilla extract 0.01-0.05 part, Lengxiang rose flower extract 0.01-0.05 part, Semen phaseoli radiati extract 0.01-0.05 part, green tea extract 0.01-0.02 part, Fructus Anisi Stellati extract 0.01-0.03 part, Radix Astragali root extract 0.01-0.02 part, Radix Ginseng extract 0.01-0.02 part, tricholoma matsutake 0.01-0.04 part, Radix Arnebiae extract 0.01-0.02 part, camomile extract 0.01-0.05 part, tree moss extract 0.01-0.03 part, Flos Lonicerae extract 0.01-0.02 part.
In described D component, the preparation method of Cordyceps extract is: get Powdered or original state Cordyceps militaris (L.) Link.sporophore 40-100g, add alkali ion water 100-300ml, mixed liquor is obtained after concussion (100-300rpm) 10-50min, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds 100-300ml alkali ion water again and carries out second extraction, extracts three times altogether successively; Merge three centrifuged supernatant and the concentrated solution of concentrated 20-30ml, the dehydrated alcohol adding concentrated solution 2-4 times amount makes it under 4 DEG C of conditions, precipitate 8-12h, incline and supernatant and obtain precipitate, this precipitate absolute ethanol washing, freeze-day with constant temperature at a certain temperature between 10 DEG C-20 DEG C, obtain polysaccharide crude, be Cordyceps extract.
Described alkali ion water is by carrying out electrolysis generation by pure water.
Described alkali ion water index is pH:9.80-13.80, current potential: >=-120mV.
The preparation method of described D component Aloe fermented product is as follows.
(1) surface cleaning: Fresh leaf of aloe clear water wash clean, described clear water can not use the tap water containing bleach, because bleach can cause aloe liquid variable color, go bad.
(2) ultraviolet radiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 5-30min.
(3) remove leaf skin: in sterilizing room, remove Fresh leaf of aloe leaf skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum.
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 10000-30000, time 0.2-5 minute); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice.
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product.
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10%(percent by volume of bacterium liquid).Fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Aloe gel fermented product.
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10%(percent by volume of bacterium liquid).Fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Folium Aloe fermented product.
Described Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, the preparation method of Flos Lonicerae extract is: get pulverous Herba Portulacae respectively, scutellariae,radix, Folium Kaki, orange peel, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Fructus Anisi Stellati, Radix Astragali root, Radix Ginseng, Trichotoma matsutake, Radix Arnebiae (Radix Lithospermi), Flos Matricariae chamomillae, tree moss, Flos Lonicerae, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, namely obtaining Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, Flos Lonicerae extract respectively.
The present invention also provides a kind of preparation method of the stock solution containing Cordyceps militaris (L.) Link. composition, comprises the following steps.
Step one, component A raw material is added blending tank by after formula metering, stir (revolution 30-100rpm) and be uniformly dispersed, be warming up to 80-90 DEG C, stirring and dissolving.
Step 2, add B component raw material stirring and dissolve, and continue after stirring and dissolving in 70-75 DEG C, be cooled to 45 DEG C.
Mix homogeneously after step 3, the 40 DEG C of preheatings of component C raw material, adds blending tank, stirs, add D component raw material and stir, vacuum outgas 10-20 minute (vacuum is 0.09-0.10MPa).
Step 4, be cooled to 30-40 DEG C, ageing 24 hours, fill, subpackage is also packed, and is finished product.
Compared with prior art beneficial effect of the present invention.
The invention provides a kind of stock solution containing Cordyceps militaris (L.) Link. composition and preparation method thereof, the described stock solution containing Cordyceps militaris (L.) Link. composition contains hydrolysis Lac regis apis albumen, Aloe fermented product, Cordyceps extract, Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, Flos Lonicerae extract 21 kinds of active component.Wherein Aloe fermented product has elimination scytitis and antibacterial action, and can significantly improve the anti-wrinkle effect of stock solution, is especially applicable to middle and aged women and uses.Herba Portulacae contains abundant SL3 fatty acid and vitamin A sample material.SL3 fatty acid forms the necessary material of cell membrane; Vitamin A sample substance maintains epithelial tissue as the normal function of skin, cornea.Orange peel extract contains terpenoid substance, plays antibacterial and natural perfume material.Containing a large amount of tannic acid in Folium Kaki, can effectively kill various antibacterial and fungus, there is effect that is clean and skin care.Lac regis apis rich in proteins, the material such as aminoacid, vitamin, lipoid, certain herbaceous plants with big flowers acetic acid, trace element, folic acid, acetylcholine, nucleic acid, has the effects such as regulation of skin metabolism and antioxidation.Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Semen phaseoli radiati extract are rich in crude polysaccharides etc.; Lengxiang rose flower extract contains spice.To sum up, the present invention has the feature of safety non-toxic containing the stock solution of Cordyceps militaris (L.) Link. composition, and raw material is easy to get, and preparation method is simple, and production cost is lower.
Detailed description of the invention
The present invention is further described below in conjunction with specific embodiment.
The invention provides a kind of stock solution containing Cordyceps militaris (L.) Link. composition, formulated by weight by following A, B, C and D component raw material.
Component A: magnificent 1% solution in pure water 40 parts, glycyrrhizic acid dipotassium 1.75 parts, trehalose 2 parts, HA(micromolecule sea) the sliding natto gum 2.1 parts of 3.2 part, threads.
B component: glycerol 2.5 parts, xanthan gum 1.7 parts, carboxymethyl R-glucan 0.15 part.
Component C: butanediol 1.3 parts, 1,2 hexanediol 0.9 part, Nipagin ester (PHENONIP) 0.3 part.
D component: 0.45 part, hydrolysis Lac regis apis albumen, hEGF (rh EGF) 0.02 part, acid fibroblast growth factor (aFGF) 0.2 part, metallothionein 0.02 part, Herba portulacae extract 0.4 part, Cordyceps extract 6.5 parts, Aloe fermented product 0.25 part, scutellariae,radix extract 0.1 part, Folium Kaki extract 0.03 part, orange peel extract 0.02 part, phellinus linteus extract 0.045 part, Salix Babylonica L.P.E 0.01 part, vanilla extract 0.03 part, Lengxiang rose flower extract 0.045 part, Semen phaseoli radiati extract 0.03 part, green tea extract 0.01 part, Fructus Anisi Stellati extract 0.01 part, Radix Astragali root extract 0.01 part, Radix Ginseng extract 0.01 part, tricholoma matsutake 0.02 part, Radix Arnebiae extract 0.02 part, camomile extract 0.04 part, tree moss extract 0.03 part, Flos Lonicerae extract 0.02 part.
In described D component, the preparation method of Cordyceps extract is: get Powdered or original state Cordyceps militaris (L.) Link.sporophore 50g, add alkali ion water 100ml, concussion (200rpm) 30min, mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds 100ml alkali ion water again and carries out second extraction, extracts three times altogether successively; Merge three extracting solution and the concentrated solution of concentrated 30ml, the dehydrated alcohol adding concentrated solution 3 times amount makes it under 4 DEG C of conditions, precipitate 10h, inclines to supernatant and obtain precipitate, this precipitate absolute ethanol washing, dry under constant temperature 10 DEG C of conditions, obtain polysaccharide crude, be Cordyceps extract.
Described alkali ion water is by carrying out electrolysis generation by pure water; Described pure water is after electrolysis, and enrichment hydroxide ion in water near negative electrode, in alkalescence, obtains alkali ion water.
The alkali ion water index that described alkali ion water outlet produces is pH:9.80-13.80, current potential: >=-120mv.
With distilled water extraction Cordyceps militaris (L.) Link.sporophore effective ingredient, extraction efficiency is 17%, and the extraction efficiency of alkali ion water is 23%.As can be seen here, described alkali ion water extraction Cordyceps militaris (L.) Link.sporophore effective ingredient has not only increased substantially extraction efficiency, also avoid the pollution because using chemical solvent to bring.
The preparation method of described D component Aloe fermented product.
(1) surface cleaning: first Fresh leaf of aloe clear water wash clean, described clear water can not use the tap water containing bleach, because bleach can cause aloe liquid variable color, go bad.
(2) ultraviolet irradiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 6min.
(3) remove leaf skin: in sterilizing room, remove Folium Aloe skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum.
(4) high speed pulverization and precipitation: leaf skin tissue mashing, become dilute slurry with high speed disintegrator (rotating speed 20000, time 3 minutes); Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice.
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product.
1. be, with aloe gel juice culture medium, with Hansenula anomala (lot number: AS2.297, Bei Nuo bio tech ltd, Shanghai) for inoculation strain, inoculum concentration 4%(percent by volume).Fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Aloe gel fermented product.
2., with Folium Aloe natural juice for culture medium, with Hansenula anomala (lot number: AS2.297, Bei Nuo bio tech ltd, Shanghai) for inoculation strain, inoculum concentration 4%(percent by volume).Fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Folium Aloe fermented product.
Prior art commonly uses Aloe original juice, and the present invention adopts Aloe fermented product, and this Aloe fermented product has following benefit compared with the Aloe original juice not through fermenting: composition is more various and molecule is less, thus is more easily absorbed by the skin.
Described Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, the preparation method of Flos Lonicerae extract is: get pulverous Herba Portulacae respectively, scutellariae,radix, Folium Kaki, orange peel, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Fructus Anisi Stellati, Radix Astragali root, Radix Ginseng, Trichotoma matsutake, Radix Arnebiae (Radix Lithospermi), Flos Matricariae chamomillae, tree moss, Flos Lonicerae, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, namely obtaining Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, Flos Lonicerae extract respectively.
The present invention also provides a kind of preparation method of the stock solution containing Cordyceps militaris (L.) Link. composition, comprises the following steps.
Step one, component A raw material is added blending tank by after formula metering, stir (revolution 50rpm) and be uniformly dispersed, be warming up to 80 DEG C, stirring and dissolving.
Step 2, add B component raw material stirring and dissolve, and continue after stirring and dissolving in 75 DEG C, be cooled to 45 DEG C.
Mix homogeneously after step 3, the 40 DEG C of preheatings of component C raw material, adds blending tank, stirs, add D component raw material and stir, vacuum outgas 15 minutes (vacuum is 0.09-0.10MPa).
Step 4, be cooled to 30 DEG C, ageing 24 hours, fill, subpackage is also packed, and is finished product.
The efficacy assessments of the present embodiment formula composition and preparation method thereof gained stock solution product.
1, practical effect evaluation: participating in stock solution effect evaluation and test interviewee number is 33 people, using the present embodiment containing stock solution every day 1 time of Cordyceps militaris (L.) Link. composition, 30 days is a course for the treatment of.Use the present embodiment containing after stock solution one course for the treatment of of Cordyceps militaris (L.) Link. composition, objective evaluation is made to this stock solution moisturizing, moisturizing, trap, stretchability, viscosity, abnormal smells from the patient, often kind of effect judgment criteria is divided into third gear, wherein moisturizing: first grade is that moistening effect is very good, second gear is that moistening effect is general, and third gear is that moistening effect is bad; Moisturizing: first grade for moisturizing effect clearly, second gear is that moisturizing effect is general, and third gear is that moisturizing effect is bad; Trap: first grade absorbs for being easy to, second gear is that assimilation effect is general, and third gear is that assimilation effect is bad; Stretchability: first grade is that stretchability is very good, second gear is that stretchability is general, and third gear is that stretchability is bad; Abnormal smells from the patient: first grade is natural comfortable, second gear is that chemical reagent is obvious, and third gear is not for having peculiar taste.Concrete evaluation result is in table 1.
Table 1: the present invention evaluates containing the stock solution result of use of Cordyceps militaris (L.) Link. composition.
| First grade | Second gear | Third gear | |
| Moisturizing | 18 | 15 | 0 |
| Moisturizing | 13 | 16 | 4 |
| Trap | 22 | 10 | 1 |
| Stretchability | 21 | 9 | 3 |
| Abnormal smells from the patient | 24 | 0 | 9 |
As shown in Table 1, the present invention has good moisturizing, moisturizing, nourishing effect containing the stock solution of Cordyceps militaris (L.) Link. composition.
2, moisture-keeping efficacy evaluation: moisture-keeping efficacy evaluation adopts external weight method, according to the difference of cosmetic composition moisture absorption, performance of keeping humidity, different wetting agent molecules is different to the active force of hydrone, absorbs moisture different with keeping the ability of moisture.Hygroscopic effect power is large, strong to water molecules power, and absorb and keep the amount of moisture also larger, closure is good, also lacking of moisture loss.Utilize adhesive tape to imitate the biomaterial such as horny layer, epidermis, cosmetics smeared by adhesive tape, the application feature of simulation pragmatize cosmetic.Under the condition of constant temperature and humidity after certain hour (4 hours), weigh sample and place the of poor quality of front and back, obtain the loss of sample size, the effect of moisturizing ingredient moisturizing can be calculated.
Directly measure for sample with the stock solution of the present embodiment containing Cordyceps militaris (L.) Link. composition, determine that this moisturizing rate containing the stock solution of Cordyceps militaris (L.) Link. composition is about 42.07% by experiment.
3, senile-resistant efficacy evaluation: senile-resistant efficacy evaluation adopts in-vitro evaluation method, and working sample is to the Scavenging activity of free radical.Comprise three indexs, be respectively: remove the mensuration of DPPH ability, remove the mensuration of superoxide anion ability and the mensuration of scavenging hydroxyl ability.
In the mensuration removing DPPH ability, the present embodiment is 21.85%-43.23% containing the clearance rate of the stock solution of Cordyceps militaris (L.) Link. composition; In the mensuration removing superoxide anion ability, the present embodiment is 20.94%-44.37% containing the stock solution of Cordyceps militaris (L.) Link. composition to the suppression ratio of superoxide anion; In the mensuration of scavenging hydroxyl ability, the present embodiment is 15.14%-24.68% containing the stock solution of Cordyceps militaris (L.) Link. composition to the clearance rate of hydroxy radical.
4, pH value measures: take bulk samples 1g(and be accurate to 0.1g), adding distil water 10g, is heated to 40 DEG C, and is constantly stirred to evenly, measures after being cooled to room temperature with pH meter.
Mensuration the present embodiment is 6.1-7.0 containing the pH value of the stock solution of Cordyceps militaris (L.) Link. composition.
Claims (5)
1. containing a stock solution for Cordyceps militaris (L.) Link. composition, formulated by following A, B, C and D component raw material:
Component A: pure water 30-45 part, glycyrrhizic acid dipotassium 0.75-3 part, trehalose 1.5-2.5 part, magnificent 1% solution in HA(micromolecule sea) 2.8-5 part, silk sliding natto gum 2-3.5 part;
B component: glycerol 1.9-2.85 part, xanthan gum 1-2.5 part, carboxymethyl R-glucan 0.1-0.3 part;
Component C: butanediol 1-2 part, 1,2 hexanediol 0.5-1.85 parts, Nipagin ester 0.15-0.4 part;
D component: hydrolysis Lac regis apis albumen 0.4-0.65 part, hEGF 0.02-0.04 part, acid fibroblast growth factor 0.15-0.5 part, metallothionein 0.015-0.03 part, Herba portulacae extract 0.1-0.4 part, Cordyceps extract 5-8.5 part, Aloe fermented product 0.1-0.5 part, scutellariae,radix extract 0.05-0.2 part, Folium Kaki extract 0.01-0.03 part, orange peel extract 0.01-0.05 part, phellinus linteus extract 0.03-0.06 part, Salix Babylonica L.P.E 0.01-0.03 part, vanilla extract 0.01-0.05 part, Lengxiang rose flower extract 0.01-0.05 part, Semen phaseoli radiati extract 0.01-0.05 part, green tea extract 0.01-0.02 part, Fructus Anisi Stellati extract 0.01-0.03 part, Radix Astragali root extract 0.01-0.02 part, Radix Ginseng extract 0.01-0.02 part, tricholoma matsutake 0.01-0.04 part, Radix Arnebiae extract 0.01-0.02 part, camomile extract 0.01-0.05 part, tree moss extract 0.01-0.03 part, Flos Lonicerae extract 0.01-0.02 part,
In described D component, the preparation method of Cordyceps extract is: get Powdered or original state Cordyceps militaris (L.) Link.sporophore 40-100g, add alkali ion water 100-300ml, mixed liquor is obtained after concussion (100-300rpm) 10-50min, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds 100-300ml alkali ion water again and carries out second extraction, extracts three times altogether successively; Merge three centrifuged supernatant and the concentrated solution of concentrated 20-30ml, the dehydrated alcohol adding concentrated solution 2-4 times amount makes it under 4 DEG C of conditions, precipitate 8-12h, incline and supernatant and obtain precipitate, this precipitate absolute ethanol washing, freeze-day with constant temperature at a certain temperature between 10 DEG C-20 DEG C, obtain polysaccharide crude, be Cordyceps extract;
The preparation method of described D component Aloe fermented product is;
(1) surface cleaning: Fresh leaf of aloe clear water wash clean;
(2) ultraviolet radiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 5-30min;
(3) remove leaf skin: in sterilizing room, remove Fresh leaf of aloe leaf skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum;
(4) high speed pulverization and precipitation: with high speed disintegrator leaf skin tissue mashing, become dilute slurry; Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice;
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product;
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10% of bacterium liquid, fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Aloe gel fermented product;
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, the inoculum concentration 1%-10% of bacterium liquid, fermentation temperature 26 DEG C-31 DEG C, fermentation time is 20-30 days, and gained fermented product is Folium Aloe fermented product;
Described Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, the preparation method of Flos Lonicerae extract is: get pulverous Herba Portulacae respectively, scutellariae,radix, Folium Kaki, orange peel, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Fructus Anisi Stellati, Radix Astragali root, Radix Ginseng, Trichotoma matsutake, Radix Arnebiae (Radix Lithospermi), Flos Matricariae chamomillae, tree moss, Flos Lonicerae, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, namely obtaining Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, Flos Lonicerae extract respectively.
2. the stock solution containing Cordyceps militaris (L.) Link. composition as claimed in claim 1, is characterized in that, formulated by following A, B, C and D component raw material:
Component A: magnificent 1% solution in pure water 40 parts, glycyrrhizic acid dipotassium 1.75 parts, trehalose 2 parts, HA(micromolecule sea) the sliding natto gum 2.1 parts of 3.2 part, threads;
B component: glycerol 2.5 parts, xanthan gum 1.7 parts, carboxymethyl R-glucan 0.15 part;
Component C: butanediol 1.3 parts, 1,2 hexanediol 0.9 part, Nipagin ester 0.3 part;
D component: 0.45 part, hydrolysis Lac regis apis albumen, hEGF 0.02 part, acid fibroblast growth factor 0.2 part, metallothionein 0.02 part, Herba portulacae extract 0.4 part, Cordyceps extract 6.5 parts, Aloe fermented product 0.25 part, scutellariae,radix extract 0.1 part, Folium Kaki extract 0.03 part, orange peel extract 0.02 part, phellinus linteus extract 0.045 part, Salix Babylonica L.P.E 0.01 part, vanilla extract 0.03 part, Lengxiang rose flower extract 0.045 part, Semen phaseoli radiati extract 0.03 part, green tea extract 0.01 part, Fructus Anisi Stellati extract 0.01 part, Radix Astragali root extract 0.01 part, Radix Ginseng extract 0.01 part, tricholoma matsutake 0.02 part, Radix Arnebiae extract 0.02 part, camomile extract 0.04 part, tree moss extract 0.03 part, Flos Lonicerae extract 0.02 part,
In described component C, the preparation method of Cordyceps extract is: get Powdered or original state Cordyceps militaris (L.) Link.sporophore 50g, add alkali ion water 100ml, concussion (200rpm) 30min, mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds 100ml alkali ion water again and carries out second extraction, extracts three times altogether successively; Merge three extracting solution and the concentrated solution of concentrated 30ml, the dehydrated alcohol adding concentrated solution 3 times amount makes it under 4 DEG C of conditions, precipitate 10h, inclines to supernatant and obtain precipitate, this precipitate absolute ethanol washing, dry under constant temperature 10 DEG C of conditions, obtain polysaccharide crude, be Cordyceps extract;
The preparation method of described D component Aloe fermented product:
(1) surface cleaning: Fresh leaf of aloe clear water wash clean;
(2) ultraviolet irradiation sterilization: after the Fresh leaf of aloe of wash clean dries, be transported into preparation of samples room, described preparation of samples room is sterilizing room (indoor have sterilizer), carries out uv light irradiation sterilization 6min;
(3) remove leaf skin: in sterilizing room, remove Folium Aloe skin, cut open crust with the blade of sterilization, and aloe gel juice is taken out; Gel juice and Ye Pi are respectively charged into Plastic Drum;
(4) high speed pulverization and precipitation: with high speed disintegrator leaf skin tissue mashing, become dilute slurry; Then precipitation process is carried out; Namely supernatant becomes Folium Aloe natural juice;
(5) preparation of Aloe fermented product: described Aloe fermented product comprises Aloe gel fermented product and Folium Aloe fermented product;
1., be culture medium with aloe gel juice, take Hansenula anomala as inoculation strain, inoculum concentration 4%, fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Aloe gel fermented product;
2., with Folium Aloe natural juice for culture medium, take Hansenula anomala as inoculation strain, inoculum concentration 4%, fermentation temperature 28 DEG C, fermentation time is 25 days, and gained fermented product is Folium Aloe fermented product;
Described Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, the preparation method of Flos Lonicerae extract is: get pulverous Herba Portulacae respectively, scutellariae,radix, Folium Kaki, orange peel, Phellinus igniarius (L. ex Fr.) Quel., salicis, Rhizoma et radix valerianae, Lengxiang rose flower, Semen phaseoli radiati, green tea, Fructus Anisi Stellati, Radix Astragali root, Radix Ginseng, Trichotoma matsutake, Radix Arnebiae (Radix Lithospermi), Flos Matricariae chamomillae, tree moss, Flos Lonicerae, mixed liquor is obtained after adding alkali ion water concussion respectively, described mixed liquor is through collected after centrifugation centrifuged supernatant, gained primary deposition thing adds alkali ion water respectively again and carries out second extraction, extract three times altogether successively, merge three centrifuged supernatant and concentrate to obtain concentrated solution, namely obtaining Herba portulacae extract, scutellariae,radix extract, Folium Kaki extract, orange peel extract, phellinus linteus extract, Salix Babylonica L.P.E, vanilla extract, Lengxiang rose flower extract, Semen phaseoli radiati extract, green tea extract, Fructus Anisi Stellati extract, Radix Astragali root extract, Radix Ginseng extract, tricholoma matsutake, Radix Arnebiae extract, camomile extract, tree moss extract, Flos Lonicerae extract respectively.
3. the stock solution containing Cordyceps militaris (L.) Link. composition as claimed in claim 1 or 2, it is characterized in that, described alkali ion water is by carrying out electrolysis generation by pure water.
4. the stock solution containing Cordyceps militaris (L.) Link. composition as claimed in claim 3, it is characterized in that, described alkali ion water index is pH:9.80-13.80, current potential: >=-120mV.
5. the preparation method of the stock solution containing Cordyceps militaris (L.) Link. composition as claimed in claim 4, is characterized in that:
Step one, component A raw material is added blending tank by after formula metering, stir (revolution 30-100rpm) and be uniformly dispersed, be warming up to 80-90 DEG C, stirring and dissolving;
Step 2, add B component raw material stirring and dissolve, and continue after stirring and dissolving in 70-75 DEG C, be cooled to 45 DEG C;
Mix homogeneously after step 3, the 40 DEG C of preheatings of component C raw material, adds blending tank, stirs, add D component raw material and stir, vacuum outgas 10-20 minute;
Step 4, be cooled to 30-40 DEG C, ageing 24 hours, fill, subpackage is also packed, and is finished product.
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