CN106727027B - Blue light resisting, oxidation resisting and free radical scavenging composition, skin care product and preparation method - Google Patents

Abstract

The invention discloses a composition for resisting blue light, resisting oxidation and removing free radicals, a skin care product and a preparation method thereof. The composition for resisting blue light, resisting oxidation and removing free radicals comprises the following components in parts by mass: 0.1-5 parts of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of alpine edelweiss extract. The compound of the marigold flower extract and the safflower seed oil can enhance the blue light filtering capability of human skin, prevent the damage of blue light to the skin, capture free radicals in human skin tissues and delay the aging caused by skin oxidation; the tea extract can make skin tender and smooth, and is helpful for cleaning skin and preventing acne; the extract of edelweiss has the effects of relieving, tranquilizing, resisting oxidation, nourishing and protecting skin.

Description

Blue light resisting, oxidation resisting and free radical scavenging composition, skin care product and preparation method

Technical Field

The invention relates to the technical field of skin care products, in particular to a composition for resisting blue light, resisting oxidation and removing free radicals, a skin care product and a preparation method thereof.

Background

Blue light is a visible light, and all light sources, whether natural or artificial, can emit blue light, which people are unknowingly exposed to. Blue light can penetrate through the whole dermis layer of the skin, but the human body cannot filter the blue light by itself and can not synthesize lutein, so that how to block the damage of the blue light to the skin becomes important. The lutein in the human skin can filter out high-energy blue light in natural sunlight and indoor light, and can capture free radicals in human skin tissues to delay aging caused by skin oxidation.

The lutein is an antioxidant with excellent performance and has the function of resisting the damage of cells and organs caused by free radicals in a human body. It has been shown that the natural antioxidant lutein prevents skin damage caused by the harmful rays of the sun. Lutein is a natural pigment widely existing in vegetables, flowers, fruits and certain algae organisms, has multiple biological activities, is widely applied, and is one of the international research hotspots at present. For example, marigold is rich in lutein.

At present, the blue light resisting, oxidation resisting and free radical eliminating compositions and skin care products on the market have unobvious effects and are easy to cause allergy. Therefore, which plant raw material is selected and safely applied to the skin care product to achieve the effects of resisting blue light, resisting oxidation and removing free radicals, so that the problem to be solved is urgently needed.

Disclosure of Invention

The invention aims to overcome the technical problems and provides a composition for resisting blue light, resisting oxidation and scavenging free radicals, a skin care product and a preparation method thereof.

The technical scheme of the invention is as follows:

the composition with blue light resistance, oxidation resistance and free radical scavenging comprises the following components in percentage by mass: 0.1-5% of a composition of marigold flower extract and safflower seed oil, 1-10% of tea extract and 1-10% of edelweiss extract.

Marigold flower extract: marigold contains abundant lutein, which is an antioxidant with excellent performance, and when added into a skin care product, the lutein can filter out high-energy blue light in natural sunlight and indoor light, prevent the damage of the blue light to the skin, capture free radicals in human skin tissues and delay aging caused by skin oxidation.

Safflower seed oil: the safflower seed oil is rich in vitamin E, is used as a natural antioxidant, has important effects on human cell division and aging delay, and has good effects on skin care: can shrink pores, relieve xerosis cutis, delay skin aging, whiten skin, repair sunburn skin, prevent skin cancer, and is suitable for various skin types.

Because the marigold flower extract is oil-soluble, the marigold flower extract is compounded with safflower seed oil with higher stability, so that the stability is improved.

Tea extract: the main component is tea polyphenol, which is applied to skin care products to make the skin tender and smooth, help to clean the skin and prevent acne, and can be used as an antioxidant of the skin care products, many skin care products contain animal and vegetable oil, especially unsaturated fat, after a long time, the oil can be oxidized and changed in taste and color under the action of air, moisture, light and trace metal ions, the tea polyphenol can act with free radicals generated during the automatic oxidation of the oil to cut off chain reaction, the rancidity and deterioration of the oil can be obviously weakened, and the quality requirement of the skin care products can be ensured.

Alpine edelweiss extract: the active ingredients of the alpine edelweiss contain rich mineral substances, natural whitening ingredients and aromatic essence, can mildly and deeply clean the skin, remove grease on the epidermis and dirt in pores, relieve the skin tension state, and have the effects of relieving, calming, resisting oxidation, nourishing and protecting the skin. The active ingredients of the alpine edelweiss are extracted and applied to daily skin care products, so that the alpine edelweiss can effectively soothe and calm the skin, harmful substances in the skin are discharged, the effects of whitening, moisturizing and resisting wrinkles are better, the skin can be thoroughly nourished and cared, and the skin can be kept bright, white and tender for a long time.

Preferably, the blue light resistant, oxidation resistant and free radical scavenging composition comprises the following components in parts by mass: 0.1-1 part of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of alpine edelweiss extract.

Preferably, in the compound of the marigold flower extract and the safflower seed oil, the mass ratio of the marigold flower extract to the safflower seed oil is 1: 19.

Preferably, the preparation method of the compound of the marigold flower extract and the safflower seed oil comprises the following steps:

mixing flos Tagetis Erectae dry powder and n-hexane at a ratio of 1:3, repeatedly extracting for 3-6 times, standing for 30min, ultrasonically oscillating for 10min, standing, and pouring out upper layer extractive solution;

mixing the extractive solutions, filtering, and rotary evaporating the filtrate at 38 deg.C under reduced pressure until no distillate is obtained to obtain brown yellow viscous oily substance;

and uniformly mixing the oily matter and the safflower seed oil according to the ratio of 1: 19.

The invention also provides a skin care product for resisting blue light, resisting oxidation and removing free radicals, which comprises the following components in percentage by mass:

phase B: 2.1-25% of composition for resisting blue light, resisting oxidation and eliminating free radicals;

and C phase: phenoxyethanol, chlorphenesin and glycerol 0.1-0.5%

Aminomethyl propanol 0.1-3%

0.01-1% of essence;

the composition for resisting blue light, resisting oxidation and removing free radicals comprises the following components in parts by mass: 0.1-5 parts of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of alpine edelweiss extract.

Preferably, the blue light resistant, oxidation resistant and free radical scavenging composition comprises the following components in parts by mass: 0.1-1 part of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of alpine edelweiss extract.

Preferably, in the compound of the marigold flower extract and the safflower seed oil, the mass ratio of the marigold flower extract to the safflower seed oil is 1: 19.

The invention also provides a preparation method of the skin care product for resisting blue light, resisting oxidation and removing free radicals, which comprises the following steps:

step S1, adding glycerol, butanediol, EDTA disodium, nicotinamide, polyglycerol-10, panthenol, caprylic/capric glyceride polyglycerol-10 esters, carbomer and deionized water in formula ratio into a vacuum stirring pot, heating to 80 ℃ to completely dissolve, and sterilizing to obtain phase D;

step S2, adding cetostearyl alcohol, cetostearyl glucoside, behenyl alcohol, polyglycerol-10 pentastearate and sodium stearyl lactylate, stearic acid, cetostearyl alcohol, shea butter, ethylhexyl palmitate, methyl hydroxybenzoate, hydrogenated polydecene, triglyceride, polydimethylsiloxane and tocopheryl acetate into an oil phase pot according to the formula ratio, heating to 80 ℃ and completely dissolving to obtain phase A;

step S3, pumping the phase A into a vacuum stirring pot under the stirring condition, uniformly mixing, and homogenizing for 10 minutes;

step S4, vacuum degassing for 20-30 minutes;

step S5, reducing the stirring speed, and cooling;

step S6, when the temperature is reduced to 45 ℃, adding the phase B and the phase C, and uniformly mixing;

and step S7, discharging, standing, filling, packaging, spraying codes and warehousing after qualified inspection.

Preferably, in step S3, the stirring speed is 60 rpm.

Preferably, in step S4, the stirring speed is 30 rpm.

Compared with the prior art, the composition capable of resisting blue light, resisting oxidation and removing free radicals and the skin care product provided by the invention have the following beneficial effects:

the composition comprises a compound of marigold flower extract and safflower oil, can supplement lutein in human skin, enhance the blue light filtering capability of the human skin, prevent the damage of blue light to the skin, capture free radicals in human skin tissues, delay aging caused by skin oxidation, shrink pores, relieve skin dryness, delay skin aging, whiten skin, repair sunburn skin and prevent skin cancer;

the composition also comprises a tea extract, so that the skin can be tender and smooth, the skin can be cleaned, acne can be prevented, the tea extract can be used as an antioxidant of a skin care product, rancidity and deterioration of grease in the skin care product can be slowed down, and the quality of the skin care product can be improved;

thirdly, the composition also comprises the extract of the velvetplant, so that the composition can mildly and deeply clean the skin, remove the grease on the epidermis and the dirt in pores, relieve the skin tension state, and has the effects of relieving, calming, resisting oxidation, nourishing and protecting the skin.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.

The invention provides a composition for resisting blue light, resisting oxidation and removing free radicals, which comprises the following components in parts by mass: 0.1-5 parts of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of alpine edelweiss extract.

The preparation method of the compound of the marigold flower extract and the safflower seed oil comprises the following steps:

mixing flos Tagetis Erectae dry powder and n-hexane at a ratio of 1:3, repeatedly extracting for 3-6 times, standing for 30min, ultrasonically oscillating for 10min, standing, and pouring out upper layer extractive solution;

mixing the extractive solutions, filtering, and rotary evaporating the filtrate at 38 deg.C under reduced pressure until no distillate is obtained to obtain brown yellow viscous oily substance;

and uniformly mixing the oily matter and the safflower seed oil according to the ratio of 1: 19.

The above composition was applied to skin care products and the formulation is shown in table 1.

Table 1 components and mass percentages of skin care products of examples 1-3 and comparative examples

The above examples 1 to 3 and comparative examples were obtained by the following preparation methods:

step S1, adding glycerol, butanediol, EDTA disodium, nicotinamide, polyglycerol-10, panthenol, caprylic/capric glyceride polyglycerol-10 esters, carbomer and deionized water in formula ratio into a vacuum stirring pot, heating to 80 ℃ to completely dissolve, and sterilizing to obtain phase D;

step S2, adding cetostearyl alcohol, cetostearyl glucoside, behenyl alcohol, polyglycerol-10 pentastearate and sodium stearyl lactylate, stearic acid, cetostearyl alcohol, shea butter, ethylhexyl palmitate, methyl hydroxybenzoate, hydrogenated polydecene, triglyceride, polydimethylsiloxane and tocopheryl acetate into an oil phase pot according to the formula ratio, heating to 80 ℃ and completely dissolving to obtain phase A;

step S3, pumping the phase A into a vacuum stirring pot under the condition that the stirring speed is 60 revolutions per minute, uniformly mixing, and homogenizing for 10 minutes;

step S4, vacuum degassing for 20-30 minutes;

step S5, reducing the stirring speed to 30 revolutions per minute, and cooling;

step S6, when the temperature is reduced to 45 ℃, adding the phase B and the phase C, and uniformly mixing;

and step S7, discharging, standing, filling, packaging, spraying codes and warehousing after qualified inspection.

Anti-blue light performance detection

The detection method comprises the following steps: the damage of blue light to Human Epidermal Keratinocytes (HEK) and Human Dermal Fibroblasts (HDF) and the protective effect of the skin care product of the present invention on the damage were investigated by measuring the survival rate and proliferation rate of metabolically active cells using CellTiter96 (MTS). Cells were cultured in 96-microwell plates, with the same number of cells in each plate.

The wells containing human epidermal cells and human dermal fiber cells were covered with a flat-bottomed sterilized plate containing the skin care product sample solution of the present invention, and subjected to LED (476nm, 1900lux) blue light irradiation for 9 hours at a distance of 30cm from the petri dish, followed by cell viability assay using CellTiter96 (MTS).

Blank control: cells were not exposed to blue light at room temperature (37 ℃).

The sample solutions were solutions of the skin care products described in examples 1-3 and comparative example, respectively, dissolved in dimethyl sulfoxide.

The average of three measurements was taken and the results are shown in Table 2.

TABLE 2

The results in Table 2 show that the skin care products of examples 1-3 containing the blue light-resistant, antioxidant, and free radical scavenging compositions have significantly greater activity in human epidermal keratinocytes and human dermal fibroblasts than the comparative examples (containing no blue light-resistant, antioxidant, and free radical scavenging compositions), indicating that the blue light-resistant, antioxidant, and free radical scavenging compositions added to the skin care products of examples 1-3 have a stronger ability to filter blue light and block the damage of blue light to the skin.

Secondly, detecting the oxidation resistance

The detection method comprises the following steps: measurements of the activity and oxidative stress of Reactive Oxygen Species (ROS) in cells were performed using the Oxiselect intracellular reactive oxygen species assay kit to detect intracellular hydroxyl, superoxide and other free radicals generated after blue light irradiation under different test conditions.

Human epidermal keratinocytes and human dermal fibroblasts were mixed together on a glass slide using DCFH-DA (2 ', 7' -dichlorofluorescein diacetate) and cultured at 37 ℃ for 1 hour. Then, the cells were washed 2-3 times with D-PBS (phosphate buffered saline), and then the culture medium solution containing the sample was added and exposed to a blue light (476nm, 1900lux)) LED lamp for 9 h. At the end of the experiment, the medium was changed to 100. mu.L of fresh medium and 100. mu.L of cell lysis buffer. After a latency period of 5min, 150. mu.L of the medium was added to a black-walled-96 microwell plate and the fluorescence was measured.

Blank control: cells were not exposed to blue light at room temperature (37 ℃).

The average of three measurements was taken and the results are shown in Table 3.

TABLE 3

Sample (I)	Fluorescence intensity of human epidermal keratinocytes	Fluorescence intensity of human dermal fiber cells
			Example 1	280	320
Example 2	350	380
			Example 3	390	400
Comparative example	700	800
			Blank control	170	190

As shown in Table 3, the skin care products of examples 1-3 containing the blue light-resistant, antioxidant, and free radical scavenging compositions showed significantly lower fluorescence intensities of human epidermal keratinocytes and human dermal fibroblasts than the comparative examples (containing no blue light-resistant, antioxidant, and free radical scavenging compositions), indicating that the blue light-resistant, antioxidant, and free radical scavenging compositions added to the skin care products of examples 1-3 had excellent antioxidant properties.

Third, detecting the performance of scavenging free radicals

The detection principle is as follows: free radicals are highly active molecules, generated by biochemical redox reactions that are part of normal cellular metabolism or as a result of exposure to environmental factors such as ultraviolet light, smoking, pollution and gamma rays, which, once generated, attack all structural components (DNA, cell membranes, proteins) in the body. DPPH is also called 1, 1-diphenyl-2-trinitrophenylhydrazine, is a very stable free radical with nitrogen as the center, and the stability of DPPH mainly comes from the steric hindrance of 3 benzene rings with resonance stabilization effect, so that unpaired electrons on the nitrogen atom in the middle cannot play the role of electron pairing. Its absolute ethyl alcohol solution is purple, and has maximum absorption at wavelength of 517nm, and its absorbance and concentration are in linear relation. When a radical scavenger is added thereto, DPPH may be combined with or substituted for the radical scavenger, whereby the radical scavenging ability can be evaluated by decreasing the number of radicals, decreasing the absorbance, and decreasing the color of the solution. Namely, the antioxidant capacity was calculated by measuring the DPPH-removing effect of the sample at a wavelength of 517 nm.

The detection method comprises the following steps: adding 4.0mL (0.02mg/mL) of absolute ethanol solution of DPPH (Japanese TOKYOKASEI) and 2.0mL of absolute ethanol diluent (3mg/mL) of each sample into a 10mL colorimetric tube in sequence, adding absolute ethanol to the scale, mixing immediately, measuring the absorbance at 517nm by using a 1cm cuvette, marking as Ai, storing in a greenhouse in dark for 30min, measuring the absorbance, marking as Aj, and performing a contrast test by using an ethanol solution only added with DPPH, wherein the absorbance is marking as A_c. The radical clearance (K) was calculated as follows:

K(％)＝[1-(Ai-Aj)/Ac]*100％

the average of three measurements was taken and the results are shown in Table 4.

TABLE 4

As can be seen from Table 4, the skin care product contains the blue light resisting, oxidation resisting and free radical scavenging composition, and the free radical scavenging rate of the skin care product is obviously higher than that of the skin care product without the blue light resisting, oxidation resisting and free radical scavenging composition, which indicates that the blue light resisting, oxidation resisting and free radical scavenging composition added in the skin care product has excellent free radical scavenging performance.

Fourth, skin elasticity detection after use

The detection method comprises the following steps: the skin care products of examples 1-3 and comparative example were used for 8 weeks in 20 volunteers, respectively, and the skin elasticity data were statistically analyzed by measuring the skin elasticity R2 value using a Cutomer (Courage & Khazaka).

The closer the value of the skin elasticity R2 is to 1, the better the skin elasticity and firmness are, and the detection results are shown in Table 5.

TABLE 5

As can be seen from Table 5, the skin care product of the present invention can significantly improve the skin elasticity of human body after adding the blue light resistant, antioxidant and free radical scavenging composition.

Compared with the prior art, the composition and the skin care product provided by the invention have the following beneficial effects:

the composition comprises a compound of marigold flower extract and safflower oil, can supplement lutein in human skin, enhance the blue light filtering capability of the human skin, prevent the damage of blue light to the skin, capture free radicals in human skin tissues, delay aging caused by skin oxidation, shrink pores, relieve skin dryness, delay skin aging, whiten skin, repair sunburn skin and prevent skin cancer;

the composition also comprises a tea extract, so that the skin can be tender and smooth, the skin can be cleaned, acne can be prevented, the tea extract can be used as an antioxidant of a skin care product, rancidity and deterioration of grease in the skin care product can be slowed down, and the quality of the skin care product can be improved;

thirdly, the composition also comprises the extract of the velvetplant, so that the composition can mildly and deeply clean the skin, remove the grease on the epidermis and the dirt in pores, relieve the skin tension state, and has the effects of relieving, calming, resisting oxidation, nourishing and protecting the skin.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (7)

1. The composition with blue light resistance, oxidation resistance and free radical scavenging is characterized by comprising the following components in parts by mass: 0.1-5 parts of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of edelweiss extract, wherein in the compound of marigold flower extract and safflower seed oil, the mass ratio of the marigold flower extract to the safflower seed oil is 1: 19;

the preparation method of the compound of the marigold flower extract and the safflower seed oil comprises the following steps:

mixing flos Tagetis Erectae dry powder and n-hexane at a ratio of 1:3, repeatedly extracting for 3-6 times, standing for 30min, ultrasonically oscillating for 10min, standing, and pouring out upper layer extractive solution;

mixing the extractive solutions, filtering, and rotary evaporating the filtrate at 38 deg.C under reduced pressure until no distillate is obtained to obtain brown yellow viscous oily substance;

and uniformly mixing the oily matter and the safflower seed oil according to the ratio of 1: 19.

2. The composition for resisting blue light, resisting oxidation and scavenging free radicals according to claim 1, characterized by comprising the following components in parts by mass: 0.1-1 part of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of alpine edelweiss extract.

3. The skin care product with blue light resistance, oxidation resistance and free radical removal is characterized by comprising the following components in percentage by mass:

phase B: 2.1-25% of composition for resisting blue light, resisting oxidation and eliminating free radicals;

and C phase: phenoxyethanol, chlorphenesin and glycerol 0.1-0.5%

Aminomethyl propanol 0.1-3%

0.01-1% of essence;

the composition for resisting blue light, resisting oxidation and removing free radicals comprises the following components in parts by mass: 0.1-5 parts of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of edelweiss extract, wherein in the compound of marigold flower extract and safflower seed oil, the mass ratio of the marigold extract to the safflower seed oil is 1: 19;

the preparation method of the compound of the marigold flower extract and the safflower seed oil comprises the following steps:

mixing flos Tagetis Erectae dry powder and n-hexane at a ratio of 1:3, repeatedly extracting for 3-6 times, standing for 30min, ultrasonically oscillating for 10min, standing, and pouring out upper layer extractive solution;

mixing the extractive solutions, filtering, and rotary evaporating the filtrate at 38 deg.C under reduced pressure until no distillate is obtained to obtain brown yellow viscous oily substance;

and uniformly mixing the oily matter and the safflower seed oil according to the ratio of 1: 19.

4. The skin care product for resisting blue light, resisting oxidation and scavenging free radicals according to claim 3, wherein the composition for resisting blue light, resisting oxidation and scavenging free radicals comprises the following components in parts by mass: 0.1-1 part of a compound of marigold flower extract and safflower seed oil, 1-10 parts of tea extract and 1-10 parts of alpine edelweiss extract.

5. A method for preparing a skin care product for resisting blue light, resisting oxidation and scavenging free radicals according to claim 3, which comprises the following steps:

step S1, adding glycerol, butanediol, EDTA disodium, nicotinamide, polyglycerol-10, panthenol, caprylic/capric glyceride polyglycerol-10 esters, carbomer and deionized water in formula ratio into a vacuum stirring pot, heating to 80 ℃ to completely dissolve, and sterilizing to obtain phase D;

step S2, adding cetostearyl alcohol, cetostearyl glucoside, behenyl alcohol, polyglycerol-10 pentastearate and sodium stearyl lactylate, stearic acid, cetostearyl alcohol, shea butter, ethylhexyl palmitate, methyl hydroxybenzoate, hydrogenated polydecene, triglyceride, polydimethylsiloxane and tocopheryl acetate into an oil phase pot according to the formula ratio, heating to 80 ℃ and completely dissolving to obtain phase A;

step S3, pumping the phase A into a vacuum stirring pot under the stirring condition, uniformly mixing, and homogenizing for 10 minutes;

step S4, vacuum degassing for 20-30 minutes;

step S5, reducing the stirring speed, and cooling;

step S6, when the temperature is reduced to 45 ℃, adding the phase B and the phase C, and uniformly mixing;

and step S7, discharging, standing, filling, packaging, spraying codes and warehousing after qualified inspection.

6. The method of claim 5, wherein the stirring speed in step S3 is 60 rpm.

7. The method of claim 5, wherein the stirring speed in step S4 is 30/min.