CN111388567B

CN111388567B - Chinese herbal medicine composition for preventing ultraviolet rays and blue light, cosmetics and preparation method

Info

Publication number: CN111388567B
Application number: CN201910001347.0A
Authority: CN
Inventors: 金恩智; 金诘; 赵一志; 边承妍; 金成珍; 李静雅
Original assignee: Shanghai Ruidian Biotechnology Co ltd
Current assignee: Shanghai Ruidian Biotechnology Co ltd
Priority date: 2019-01-02
Filing date: 2019-01-02
Publication date: 2022-10-28
Anticipated expiration: 2039-01-02
Also published as: CN111388567A

Abstract

The invention provides a Chinese herbal medicine composition for preventing ultraviolet and blue light, which comprises the following components in part by weight: 1-6 parts of artemisia capillaris extract, 2-7 parts of white mulberry root-bark extract and 2-8 parts of black tea extract. The composition can also be used for preparing cosmetics, and the composition and the cosmetics have the effects of ultraviolet resistance, blue light resistance and whitening, and are convenient to prepare and low in cost.

Description

Chinese herbal medicine composition for preventing ultraviolet rays and blue light, cosmetics and preparation method

Technical Field

The invention relates to a composition, in particular to a Chinese herbal medicine composition.

Background

Medium-wave ultraviolet (UVB for short) can directly act on DNA to make it absorb energy and produce mutation; reactive Oxygen Species (ROS) generated by UVB and long-wave ultraviolet rays (UVA for short, the wavelength is 320-420 nm, and the UVA is also called long-wave black spot effect ultraviolet rays) can indirectly damage cell nucleus and mitochondrial DNA, so that abnormal cell functions or apoptosis can be caused. ROS generated by UV irradiation can oxidize and damage proteins and lipids, causing abnormalities in the corresponding functions and structures. Ultraviolet radiation can affect skin pigment metabolism: the immediate reaction is a reactive increase in synthesis and redistribution of melanin; the delayed response is an increase in the number and viability of melanocytes. Thus, ultraviolet light can cause skin tanning, premature aging, sallow complexion, blotchiness, wrinkling and even carcinogenesis.

Blue light is high-energy visible light with a wavelength of 380-500 nm and extremely strong penetrating power. In experiments, the energy of high-energy short-wave blue light with the wavelength of 410 nm is 1/3 of UVA with the wavelength of 380 nm, and the energy of high-energy short-wave blue light with the wavelength of 3 nm is equivalent to 1 hour of sunshine after a high-brightness screen is used, so that your eyes can be sore and the skin can be dry, and the high-energy short-wave blue light is excited to generate a brown pigment called Maillarin.

In recent years, with the acceleration of people's life and work pace, the aggravation of environmental pollution and the increasing popularization and use of computers and mobile phones, people face more and more skin problems, especially dark skin color, pigmentation and the like become common problems, and ultraviolet-proof and blue-proof skin care cosmetics also become a hotspot in the field of skin care product development. With the increasingly mature consumption concept of people, the attention of consumers on skin care cosmetics is focused not only on the efficacy of the cosmetics, but also on the safety of the cosmetics. Compared with chemical components with safety risks such as irritation, the natural whitening agent derived from Chinese herbal medicines has the advantages of safety, mildness, durability, high efficiency and the like, and becomes a great trend of research and development of skin care cosmetics at present. Some traditional Chinese medicine compositions reported at present have a common characteristic and the greatest defect, namely the composition is complex and can only resist ultraviolet rays generally.

Therefore, the development of mild ultraviolet-proof and blue-light-proof Chinese herbal medicine composition which is nontoxic and nonirritating to human skin and cosmetics thereof is urgently needed in the field.

Therefore, there is a strong need in the art to develop natural UV-and blue-light-resistant compositions.

Disclosure of Invention

The invention aims to provide a natural ultraviolet-resistant and blue-light-resistant composition, and the composition also has a whitening effect.

In a first aspect of the present invention, there is provided a Chinese herbal composition for protecting against ultraviolet and blue light, the composition comprising: 1-6 parts of artemisia capillaris extract, 2-7 parts of white mulberry root-bark extract and 2-8 parts of black tea extract.

The above extract is organic extract.

The above components are extracted with C2-C6 alcohol or GTCC.

The mass ratio of the artemisia capillaris extract to the black tea extract to the white mulberry root-bark extract is 3:3:4.

the second invention of the present invention provides a method for preparing the above Chinese herbal medicine composition, comprising the steps of:

(1) Drying the raw materials, and respectively crushing;

(2) Adding the crushed raw materials into C2-C6 alcohol or GTCC in an amount which is 10-30 times of the mass of all the raw materials according to the mass ratio, and stirring for 0.5-1.5h;

(3) Filtering with quantitative filter paper to obtain composition filtrate.

The preparation method also comprises the following steps:

(4) Sterilizing the composition filtrate at high temperature.

The autoclaving conditions were 116 deg.C, 30 min, and 1.4kg/cm ² 。

Adding the crushed raw materials into butanediol which is 10-30 times of the mass of all the raw materials according to the mass ratio, and stirring for 0.5-1.5h in the step (2); the aperture of the quantitative filter paper is 1-3 microns.

The preparation method of the Chinese herbal medicine composition (the mass ratio of the artemisia capillaris extract to the black tea extract to the white mulberry root-bark extract is 3):

(1) Drying the raw materials, and respectively crushing;

(2) Respectively adding the raw materials into C2-C6 alcohol or GTCC in an amount which is 10-30 times of the raw materials by mass, and stirring for 0.5-1.5h;

(3) Filtering with quantitative filter paper to obtain filtrates of the raw materials, and mixing the filtrates according to mass ratio to obtain composition filtrate.

Adding the raw materials into butanediol with the mass of 10-30 times of that of the raw materials in the step (2) respectively, and stirring for 0.5-1.5h; the aperture of the quantitative filter paper is 1-3 microns.

The third invention provides an ultraviolet-proof and blue-light-proof cosmetic, which comprises the Chinese herbal medicine composition with the mass percentage of 0.5-10%.

Preferably, the mass percentage of the Chinese herbal medicine composition in the cosmetics is 1-5%.

The product has the main advantages that the product adopts natural substances, is non-toxic and non-irritant to human skin, and can provide mild anti-ultraviolet and anti-blue-light Chinese herbal medicine composition and cosmetics thereof for consumers. Moreover, the preparation method comprehensively considers the extraction efficiency and the product cost, and has the advantages of simplicity, high efficiency and lower cost.

The blue light blocking effect of the present invention is attributed to the purely natural coloring matter component. Since natural coloring matter is a water-insoluble and water-insoluble substance, there is a problem that the extraction yield is low when purified water is used for extraction. However, the extraction with ethanol has a limitation that the content of the extract in the cosmetic cannot be excessively high, and the concentration of ethanol is required, which leads to an increase in cost.

The invention is preferably extracted with BG (Butylene glycol), but ethanol, propylene glycol, dipropylene glycol, hexylene glycol and GTCC (caprylic/capric triglyceride) may also be used.

The artemisia capillaris can grow to 1-1.8 m like trees in deep mountains with the altitude of more than 300. Unlike common mugwort, it has large leaves, strong fragrance, and contains a large amount of vitamins, iron and calcium. It is a plant widely used in folk therapy and can be used for treating various symptoms such as jaundice. Because it is rich in vitamins and minerals, and is powerful, it can continue to survive the entire winter. Also known as artemisia annua. It is rich in beta-carotene and various vitamins, and has effects of improving skin elasticity and luster, eliminating active oxygen, and preventing skin aging.

Cortex Mori is dried root bark of Morus alba L of Moraceae. Digging root part from late autumn to spring before germination, scraping yellow brown coarse bark, longitudinally cutting, stripping root bark, and sun drying. Mulberry, a combination of medicine () and tree (treeberry), symbolizes a holy tree in the east. Cortex Mori can be used for treating lung heat and asthma caused by waste heat, and has diuretic effect. Can be used for treating acute pyelonephritis and edema, lowering blood pressure, and lowering blood sugar. Can also be used for treating harmful hepatitis. It is reported that the drugs have diuretic, hypotension, sedative, analgesic, antipyretic and other pharmacological actions.

The shape is in the shape of a twisted reel, a groove or a plate. The outer surface is white or yellowish white and is relatively flat, and orange yellow or brown yellow scale-shaped coarse skin is remained; the inner surface is yellow-white or gray-yellow and has fine longitudinal lines. Light weight, tough quality, strong fiber, difficult breaking, easy longitudinal tearing and dust flying during tearing. Also called cortex Mori, and cortex Mori.

Black tea is prepared by using new tooth leaves of a black tea tree as a raw material and refining the black tea through a series of processes such as withering, rolling (cutting), fermentation, drying and the like. The main producing areas are China, srilanka, india, indonesian and Kenya. The fermentation in black tea manufacture is not a microbial fermentation in the brewing industry, but is a weak oxidation of the oxidase enzymes contained in the tea leaves. It is defined in korean industrial standard (KS) as tea leaves prepared by using tea leaves suitable for drinking, such as tea leaves belonging to the family theaceae, as a raw material through initial drying (withering), rolling, fermentation, baking, and re-baking. The black tea contains various nutrient components, wherein the most of the nutrient components are tea polyphenol, and the tea has strong antioxidation function and can inhibit the activity of harmful oxygen for promoting aging. The black tea can help gastrointestinal digestion, promote appetite, promote urination, eliminate edema and strengthen heart function. The flavonoid compounds rich in black tea can eliminate free radicals, has antacid effect, and can reduce incidence of myocardial infarction. The tea polyphenols can also prevent cholesterol absorption by digestive tract, and reduce cholesterol content in blood. In addition, tea polyphenols can prevent platelet aggregation, inflammation, obesity, diabetes, inflammation and dental caries.

The combination of the three natural components achieves unexpected technical effects, and particularly when the mass ratio of the artemisia capillaries, the white mulberry root-bark and the black tea is 3. The composition can also be used for addition to cosmetics.

Drawings

FIG. 1 is a schematic view of the extraction procedure of Artemisia capillaris Thunb extract.

FIG. 2 is a schematic view of the common extraction steps of Artemisia capillaris Thunb, cortex Mori and black tea.

FIG. 3 is an absorbance spectrum of 1% of the organic extract of Artemisia capillaris Thunb.

FIG. 4 is an absorbance spectrum of 1% of the organic extract of cortex Mori.

FIG. 5 is an absorbance spectrum of 1% of black tea organic extract.

Fig. 6 is the absorbance of artemisia capillaris, white mulberry root-bark, black tea blend extract (3

Detailed Description

Hereinafter, the present invention is described in more detail and specifically with reference to examples, but the following examples are not intended to limit the present invention.

EXAMPLE 1 extraction of three herbs separately 1

As shown in FIG. 1, oven-dried herba Artemisiae Scopariae was pulverized, added with 40% butyl ene glycol (butanediol) 10 times of the weight of herba Artemisiae Scopariae, stirred and extracted at room temperature for one hour at a stirring speed of 300rpm, filtered with 5C filter paper (pore size of 1 μm) to obtain filtrate, and sterilized at 116 deg.C for 30 minutes under 1.4kg/cm ³ . Black tea extract and cortex Mori extract can be obtained by the same method.

EXAMPLE 2 extraction of three herbs separately 2

Pulverizing dried herba Artemisiae Scopariae, adding 40% (v/v) butyl ethylene glycol 15 times of herba Artemisiae Scopariae, stirring at 300rpm for half an hour at normal temperature, and filtering with 5C filter paper (pore size 1 μm) to obtain filtrate. Black tea extract and cortex Mori extract can be obtained by the same method.

EXAMPLE 3 extraction of three herbs 3

Dried artemisia capillaris, white mulberry root bark and black tea were pulverized (mass ratio 3. Black tea extract and cortex Mori extract can be obtained by the same method.

EXAMPLE 4 extraction of three herbs separately 4

Pulverizing dried herba Artemisiae Scopariae, adding 40% (v/v) butyl ethylene glycol (butanediol) 30 times of herba Artemisiae Scopariae, stirring and extracting at 300rpm for half an hour at normal temperature, and filtering with 5C filter paper (pore size 1 μm) to obtain filtrate. Black tea extract and cortex Mori extract can be obtained by the same method.

Example 5 Combined extracts of three herbs 5

As shown in fig. 2, dried artemisia capillaris, white mulberry root bark and black tea were pulverized (mass ratio 1.

EXAMPLE 6 Combined extracts of three herbs 6

Dried artemisia capillaris, white mulberry root bark and black tea are pulverized (mass ratio 5:7: 8), and then three Chinese herbal medicines are added, wherein the mass of the three Chinese herbal medicines is 20 times, 40% of butyl ene glycol (butanediol) is added, and the mixture is stirred and extracted for one hour at normal temperature, and then filtered by 5C filter paper to obtain filtrate.

EXAMPLE 7 Combined extracts of three herbs 7

Dried artemisia capillaris, white mulberry root bark and black tea are pulverized (mass ratio 3: 4), and then three Chinese herbal medicines with the mass of 20 times of 40 percent are added, and the mixture is stirred and extracted for one hour at the normal temperature state, and then filtered by 5C filter paper to obtain filtrate.

Example 8 Combined extracts of three herbs 8

Dried artemisia capillaris, white mulberry root bark and black tea are pulverized (mass ratio 6: 7), and then added with 40% butyl ene glycol 20 times the mass of three Chinese herbal medicines, stirred and extracted for one hour at normal temperature, and then filtered with 5C filter paper to obtain filtrate.

EXAMPLE 9 Combined extracts of three herbs 9

Pulverizing oven-dried herba Artemisiae Scopariae, cortex Mori, and black tea (mass ratio 3:4: 3), adding 40% butyl ene glycol (butanediol) 20 times the mass of the three Chinese medicinal materials, extracting under stirring at room temperature for one hour, and filtering with 5C filter paper to obtain filtrate.

EXAMPLE 10 absorbance measurements of the extracts

1. Absorbance measurements of compositions of different mass ratios

Virgate wormwood, white mulberry root-bark and black tea were mixed and extracted in the mass ratio of 2. As shown in table 1.

TABLE 1 Absorbance assay data for each composition

2. Absorbance detection of organic extracts of different compositions

Wavelength of ultraviolet light	Degree of absorption
		270nm	0.235
290nm	0.570
		300nm	0.630
350nm	0.394

TABLE 2 Absorbance data of 1% for organic extracts of Artemisia capillaris Thunb

Wavelength of ultraviolet light	Absorbance of the solution
		270nm	0.241
290nm	0.564
		300nm	0.647
350nm	0.368

TABLE 3 Absorbance data of 1% of organic extracts of cortex Mori

Wavelength of ultraviolet light	Absorbance of the solution
		270nm	0.346
290nm	0.482
		300nm	0.516
350nm	0.885

Table 4 absorbance data of organic extracts of black tea at 1%

3. Absorbance measurements of extracts of different mass concentrations at a fixed mass ratio

Ultraviolet ray&Blue light wavelength	Absorbance of the solution
		270nm	1.077
290nm	2.312
		300nm	>2.5
350nm	>2.5
		405nm	>2.5
450nm	2.099

Table 5 absorbance data of 1% of organic extracts of composition artemisia capillaris taro, mulberry bark, black tea (3

Table 6: absorbance data for extracts of the invention at different mass concentrations

From tables 1-5, it can be seen that the compositions of the three extracts, tarragon to mulberry bark to black tea, 4-6:7-8:7-8, are all effective in absorbing UV and blue light, especially tarragon to mulberry bark to black tea, when extracted at a ratio of 3. Moreover, as can be seen from table 6, the ratio of artemisia capillaris to white mulberry to black tea extract of 3.

Therefore, the absorption of ultraviolet and blue wavelengths is greatly increased by the synergistic effect of the three raw materials. In addition, it was confirmed that the absorbance of blue light at a wavelength of 460nm, which is associated with a biorhythm, was decreased.

We observe the change in absorption at wavelength according to the change in the raw material ratio.

Example 11 skin safety test

Finally, mixing the artemisia capillaris: white mulberry root-bark: the black tea (3.

The sample was applied to the skin with a PIN Chamber (skin irritation test patch) for 24 hours after the reagent was diluted to the designated concentration, and skin irritation was observed 1 hour after and 24 hours after the removal of the skin irritation test patch. It was found that all test subjects showed no skin irritation at all concentrations tested.

Table 4 skin safety test results

Skin irritation criteria:

no irritation

Slight irritation

Severe irritation

Very severe irritation

Example 12 whitening Effect test

The whitening effect test is carried out on 1% of the artemisia capillaris, the white mulberry root-bark and the black tea (3. The composition of the invention can not only prevent ultraviolet and blue light, but also has better whitening effect.

The test procedure was as follows:

1) The extract was diluted with 0.1M phosphate buffer (pH 7.0) and used as a sample solution.

2) 200. Mu.l of a 500. Mu.l sample solution of 0.1M phosphate buffer solution (pH 7.0) and 200. Mu.l of a 5mM L-DOPA (L-3,4-dihydroxyphenylalanine) solution were put into a glass test tube and reacted at 37 ℃ for 5 minutes.

3) To the solution was added 100. Mu.l of mercury black tyrosinase, reacted at room temperature for 5 minutes, and the absorbance at 475nm was measured.

4) 0.1M phosphate buffer (pH 7.0) was then added to another tube as a control solution.

Further, it should be understood that the above embodiments are only intended to illustrate the technical solutions of the present invention, and not to limit the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that various modifications or equivalent substitutions may be made thereto without departing from the scope and spirit of the present invention.

Claims

1. The Chinese herbal medicine composition for preventing ultraviolet rays and blue light is characterized by comprising the following components in parts by weight: 3 parts of artemisia capillaris extract, 4 parts of cortex mori extract and 3 parts of black tea extract, wherein the extracts are butanediol extracts; the preparation method of the Chinese herbal medicine composition comprises the following steps:

(1) Drying the raw materials, and respectively crushing;

(2) Adding the crushed raw materials into butanediol which is 10-30 times of the mass of all the raw materials according to the mass ratio, and stirring for 0.5-1.5h;

(3) Filtering with quantitative filter paper to obtain composition filtrate, wherein the pore diameter of the quantitative filter paper is 1-3 μm;

(4) Sterilizing the composition filtrate at high temperature; the high temperature sterilization conditions are 116 ℃,30 minutes and 1.4kg/cm of pressure ² 。

2. An ultraviolet-proof blue-light-proof cosmetic, which is characterized by comprising 0.5-10% of the Chinese herbal medicine composition according to claim 1 in percentage by mass.