CN108785214B

CN108785214B - Blue light damage resistant compound and application thereof in cosmetics

Info

Publication number: CN108785214B
Application number: CN201811098338.XA
Authority: CN
Inventors: 孔令姗; 吴震; 郑雄健; 谢振荣
Original assignee: Shanghai Lanhai Bio Tech Co ltd
Current assignee: Shanghai Lanhai Bio Tech Co ltd
Priority date: 2018-09-20
Filing date: 2018-09-20
Publication date: 2021-06-04
Anticipated expiration: 2038-09-20
Also published as: CN108785214A

Abstract

The invention discloses a compound for resisting blue light injury, and the active ingredients of the compound are prepared by compounding traditional Chinese medicines of bamboo leaves, golden cypress, sophora flower, grape seeds, giant knotweed rhizome, liquorice and centella. The invention also provides a preparation method of the blue light damage resistant compound, which comprises the steps of weighing the components of the traditional Chinese medicine raw materials, and preparing an extract through extraction, filtration, sedimentation, centrifugation, microfiltration membrane treatment and concentration process stages; and dissolving the extract into liquid with the mass fraction of 0.1-10%, thus obtaining the blue light damage resistant compound. The compound capable of resisting blue light damage has strong absorption in the blue light region waveband of 400-480 nm, and has the effects of oxidation resistance and repair. The blue light resistant compound provided by the invention has an obvious effect, is high in safety by combining a traditional Chinese medicine technology, and can be applied to cosmetics.

Description

Blue light damage resistant compound and application thereof in cosmetics

Technical Field

The invention belongs to the field of traditional Chinese medicines and daily chemicals, and relates to a cosmetic, in particular to an anti-blue-light-damage compound and application thereof in cosmetics.

Background

The skin can be photodamaged after being exposed to sunlight for a long time, and consumers mainly pay attention to damage of ultraviolet rays in the sunlight to the skin of a human body in the past, but neglect the effect of visible light. In daily life of people, except blue light in sunlight, mobile phones, computers, televisions and other artificial light sources such as LED lamps can also generate blue light, so that the exposure time of people in the blue light is prolonged. Research summary of damage research on blue light caused by Xiaiting and Tianyan is carried out (Xiaiting, Tianyan, research progress of damage and protection of blue light on skin [ J ]. Lighting engineering report, 2017,28(6): 20-23).

In recent years, researches show that the damage of visible light, particularly blue light (with the wavelength of 400-480 nm) to the skin is obvious. The blue light is the wave band with the shortest wavelength and the highest energy in the visible spectrum, has stronger penetrating power than ultraviolet rays, and can also generate damage similar to the ultraviolet rays on human skin.

Researches report that blue light can penetrate skin tissues to enable cells to generate oxidative stress and cytotoxicity, reduce the oxidation resistance of fibroblasts, inhibit the proliferation of the fibroblasts, reduce extracellular matrix and cause photoaging; furthermore, it may affect the growth, metabolism, DNA damage of epithelial cells and may even lead to cell dysfunction and apoptosis of the ductal cells.

At present, the blue light damage resistant cosmetics and functional raw material products in the market are few, and the attention and the types are few. Therefore, it is necessary to develop a product for resisting blue light damage.

Disclosure of Invention

Aiming at the technical problems in the prior art, the invention provides a blue light damage resistant compound and an application thereof in cosmetics, and the blue light damage resistant compound and the application thereof in the cosmetics aim at solving the technical problem that the cosmetics in the prior art are poor in blue light effect in visible light resistance.

The invention provides an anti-blue-light injury compound, and the active ingredients of the compound are prepared by compounding traditional Chinese medicines of bamboo leaves, golden cypress, sophora flower, grape seeds, giant knotweed rhizome, liquorice and centella.

Further, the compound is prepared from the following raw materials in parts by weight: 1-20 parts of bamboo leaves, 1-20 parts of golden cypress, 1-20 parts of sophora flower, 1-20 parts of grape seeds, 1-20 parts of giant knotweed rhizome, 0.5-10 parts of liquorice and 0.5-10 parts of centella.

Further, the compound is prepared from the following raw materials in parts by weight: 15 parts of bamboo leaves, 12 parts of golden cypress, 12 parts of sophora flower, 8 parts of grape seeds, 8 parts of giant knotweed, 8 parts of liquorice and 6 parts of centella.

Further, the compound is prepared from the following raw materials in parts by weight: 16 parts of bamboo leaves, 12 parts of golden cypress, 12 parts of sophora flower, 8 parts of grape seeds, 8 parts of giant knotweed, 6 parts of liquorice and 6 parts of centella.

Further, the compound is prepared from the following raw materials in parts by weight: 10 parts of bamboo leaves, 15 parts of golden cypress, 15 parts of sophora flower, 6 parts of grape seeds, 6 parts of giant knotweed, 8 parts of liquorice and 5 parts of centella.

Further, the compound is prepared from the following raw materials in parts by weight: 8 parts of bamboo leaves, 16 parts of golden cypress, 16 parts of sophora flower, 8 parts of grape seeds, 8 parts of giant knotweed, 3 parts of liquorice and 7 parts of centella.

The invention also provides a preparation method of the blue light damage resistant compound, which comprises the following steps:

1) weighing the traditional Chinese medicine raw materials, adding an alcohol solvent with the mass fraction of 30-80% according to the mass ratio of 1: 5-1: 30 of the material liquid, extracting for 2 hours at 70 ℃ under normal pressure, filtering, settling for 1-7 days at 10 ℃, centrifuging and filtering at 2800r/min for 20min, treating with a microfiltration membrane, distilling under reduced pressure, and heating the concentrated solution at 50 ℃ to obtain an extract for later use;

2) taking out the extract prepared in the step 1), adding an alcohol solvent, and preparing into liquid with the mass percentage concentration of 0.1-10%, namely the blue light damage resistant compound.

Further, the alcohol solvent is one or a mixture of more than two of ethanol, butanediol, propylene glycol, glycerol, isopropylene glycol and dipropylene glycol.

The invention also provides a blue light damage resistant cosmetic containing the blue light damage resistant compound.

Furthermore, in the cosmetic, the concentration of the blue light resisting compound is 0.1-20% by mass.

Furthermore, in the blue light damage resistant cosmetic, the blue light damage resistant compound is contained in an amount of 0.1-10% by mass.

Specifically, the blue light damage resistant cosmetic is composed of a blue light damage resistant compound, common cosmetic auxiliaries and a carrier.

Among the raw materials selected by the compound for resisting the blue light injury, the bamboo leaves have the effects of clearing heat, relieving restlessness, promoting the production of body fluid and promoting urination; cortex Phellodendri has effects of clearing heat, eliminating dampness, purging pathogenic fire, removing steam, removing toxic substance, and treating sore; flos Sophorae Immaturus has effects of clearing heat, cooling blood, and stopping bleeding; the grape seed has antioxidant, free radical scavenging, and antiinflammatory effects; the giant knotweed has the effects of clearing away heat and toxic materials, dissipating blood stasis and relieving pain; the licorice has the effects of clearing away heat and toxic materials, eliminating phlegm and relieving cough; centella has the functions of clearing heat, promoting diuresis, relieving swelling and detoxifying.

Compared with the prior art, the invention has remarkable technical progress. The blue light damage resistant compound provided by the invention has a strong absorption effect in a blue light region wavelength range of 400-480 nm, and the compound has oxidation resistance and repair effects. The blue light damage resistant compound is combined with Chinese traditional medicine components and concepts, has high safety and convenient use, and has better prospect when being applied to cosmetics.

The present invention will be further described with reference to specific embodiments.

Drawings

FIG. 1 is a graph of transmittance at 400-500nm for a 1% composite-propylene glycol solution with blue light damage resistance.

FIG. 2 shows the DPPH radical clearance by different mass fractions of the anti-blue-light damage complex.

Detailed Description

The present invention will be further illustrated by the following examples, and it will be apparent that the scope of the present invention is not limited to the following examples. Based on the embodiments of the present invention, those skilled in the art can modify or appropriately combine the methods and applications described herein without departing from the spirit and scope of the present invention.

The invention has been described generally and/or specifically with respect to materials and methods used in the experiments, and many materials and procedures used are known in the art and are described in as great a detail as possible; the used traditional Chinese medicine raw materials provide products for the company and/or purchase products on the market.

Example 1: blue light damage resistant complexes

The active ingredient of the blue light damage resistant compound is prepared from the following raw materials in parts by mass: 16 parts of bamboo leaves, 12 parts of golden cypress, 12 parts of sophora flower, 8 parts of grape seeds, 8 parts of giant knotweed, 6 parts of liquorice and 6 parts of centella.

The preparation method of the blue light damage resistant compound comprises the following steps:

1) accurately weighing the traditional Chinese medicine raw materials, adding an alcohol solvent with the mass fraction of 80% according to the mass ratio of the material liquid of 1:5, extracting for 2h at 70 ℃ under normal pressure, filtering with a filter cloth, settling for 5 days at 10 ℃, centrifuging and filtering at 2800r/min for 20min, treating with a microfiltration membrane, distilling under reduced pressure, and heating the concentrated solution at 50 ℃ to obtain an extract for later use.

2) Taking out the extract prepared in the step 1), adding an alcohol solvent, and preparing into liquid with the content of 2%, namely the blue light damage resistant compound.

Example 2: blue light damage resistant complexes

The active ingredient of the blue light damage resistant compound is prepared from the following raw materials in parts by mass: 10 parts of bamboo leaves, 15 parts of golden cypress, 15 parts of sophora flower, 6 parts of grape seeds, 6 parts of giant knotweed, 8 parts of liquorice and 5 parts of centella.

1) accurately weighing the traditional Chinese medicine raw materials, adding 70% by mass of alcohol solvent according to the feed liquid mass ratio of 1:15, extracting at 70 ℃ for 2h under normal pressure, filtering with filter cloth, settling at 10 ℃ for 3 days, centrifuging at 2800r/min for 20min, treating with microfiltration membrane, distilling under reduced pressure, and heating the concentrated solution at 50 ℃ to obtain extract for later use.

2) Taking out the extract prepared in the step 1), adding an alcohol solvent, and preparing into liquid with the content of 5%, namely the blue light damage resistant compound.

Example 3: blue light damage resistant complexes

The active ingredient of the blue light damage resistant compound is prepared from the following raw materials in parts by mass: 8 parts of bamboo leaves, 16 parts of golden cypress, 16 parts of sophora flower, 8 parts of grape seeds, 8 parts of giant knotweed, 3 parts of liquorice and 7 parts of centella.

1) accurately weighing the traditional Chinese medicine raw materials, adding an alcohol solvent with the mass fraction of 55% according to the mass ratio of the material liquid of 1:25, extracting for 2h at 70 ℃ under normal pressure, filtering with a filter cloth, settling for 1 day at 10 ℃, centrifuging and filtering at 2800r/min for 20min, treating with a microfiltration membrane, distilling under reduced pressure, and heating the concentrated solution at 50 ℃ to obtain an extract for later use.

2) Taking out the extract prepared in the step 1), adding an alcohol solvent, and preparing into liquid with the content of 10%, namely the blue light damage resistant compound.

Example 4: blue light absorption test

A study product of the blue light damage resistant complex prepared in experimental example 2. Accurately weighing the blue light damage resistant compound, and adopting propylene glycol as a solvent to prepare liquid with the mass fraction of 1% to scan in a blue light region within the range of 400-480 nm. The results are shown in FIG. 1 below.

Example 5: anti-blue light damage complex scavenging free radical assay

The blue light damage resistant complex prepared in example 2 was used as a research product. Accurately weighing the blue light damage resistant compound, and preparing liquid with different mass fractions of 0.1%, 0.2%, 0.5%, 1% and 2% by using propylene glycol as a solvent to test the DPPH scavenging capacity of free radicals.

The experimental method comprises the following steps: 3mL of polysaccharide solutions of different concentrations were placed in a test tube and 1mL of DPPH (1.0X 10) was added^-4mol/L, 50% ethanol), mixing uniformly, reacting for 30min in the dark, and measuring the absorbance at 517nm As, the absorbance of 1mL of 50% ethanol instead of DPPH As Ac, and the absorbance of 3mL of 50% ethanol instead of the blue light damage resistant compound As Ao. Triplicate replicates were made for each sample and the average was taken. Clearance was calculated as follows:

) ×100

the results are shown in FIG. 2 below:

as can be seen from fig. 1 and 2, the transmittance in the blue light region is low, i.e. the absorption of blue light is strong, when 1% of the mass fraction of the blue light damage resistant compound is added; and the clearance rate of 0.2 percent of the blue light damage resistant compound on the DPPH reaches 91.6 percent; has strong blue light absorption and oxidation resistance.

Example 6: anti-blue light damage gel

The blue light damage resistant complex prepared in example 2 was used as a research product. The formulations are referred to in the following table:

the preparation method comprises the following steps:

1. dispersing carbomer 980 and sodium hyaluronate in glycerol, adding water and simultaneously raising the temperature to 85 ℃;

2. cooling to 50-55 deg.C;

3. adding phase B, stirring, adding phase C, stirring, and discharging with pH value controlled at 5.5-6.5.

Example 7: cream for resisting blue light damage

note: -means not added.

The preparation method comprises the following steps:

1. dispersing carbomer 980 in butanediol, adding water and simultaneously raising the temperature to 85 ℃;

2. adding the rest components of the phase B, and uniformly stirring;

3. completely dissolving the phase A at 85 ℃, and uniformly stirring;

4. adding the phase A into the phase B, stirring and homogenizing, defoaming and cooling to 50 ℃;

5. adding phase C, stirring, adding phase D, stirring, and controlling pH at 5.5-6.5.

Example 8: experimental test for blue light damage resistance

The blue light damage resistant creams (formula one, formula two, formula four) formulated in example 7 above were used for testing studies.

The method comprises the following steps:

selecting 10 test volunteers, and testingSelecting 4 test areas of 3 × 3cm on the back of the patient, selecting one area as a reference, and uniformly smearing 3mL of cream on the rest 3 areas respectively; irradiating with LED lamp for 1h, containing blue light (wavelength center 450 nm) and 150J/m²The amount of pigmentation (MPD) (including melanin and heme) was measured using the CK company instrument MDD4-Mexameter MX18, and the results are shown in Table 1 below.

The second method comprises the following steps:

selecting 10 test volunteers, selecting 4 test areas of 3 × 3cm on the back of the test volunteer, and irradiating with LED lamp for 1 hr to obtain test area containing blue light (wavelength center 450 nm) of 150J/m²One area was selected as a control, 3mL of formula I, formula II, and formula IV cream was uniformly applied to the remaining 3 areas for 28 days, 2 times per day, and the amount of pigmentation (MPD) (including melanin and heme) was measured using CK corporation's instrument MDD4-Mexameter MX18, the results of which are shown in Table 2 below.

TABLE 1

Group of	Control	Formulation I	Formulation II	Formulation IV
					MPD	7.49	6.82	5.31	3.89

TABLE 2

Group of	Control	Formulation I	Formulation II	Formulation IV
					Reduction ratio of MPD%	6.92	9.34	21.46	54.72

As can be seen from tables 1 and 2, the anti-blue light damage complex provided by the invention has good defense and repair effects on skin damage caused by blue light.

Claims

1. A blue light damage resistant composite characterized by: the compound is prepared from the following raw materials in parts by weight: 10 parts of bamboo leaves, 15 parts of golden cypress, 15 parts of sophora flower, 6 parts of grape seeds, 6 parts of giant knotweed, 8 parts of liquorice and 5 parts of centella;

1) accurately weighing the raw materials, adding 70 wt% alcohol solvent at 70 deg.C according to the material liquid mass ratio of 1:15

Extracting under normal pressure for 2h, filtering with filter cloth, settling at 10 deg.C for 3 days, centrifuging at 2800r/min for 20min, treating with microfiltration membrane, distilling under reduced pressure, and heating concentrated solution at 50 deg.C to obtain extract;

2) taking out the extract prepared in the step 1), adding an alcohol solvent, and preparing into liquid with the mass percentage concentration of 5%, namely the blue light damage resistant compound.

2. A blue light damage resistant cosmetic characterized by: the blue light damage resisting compound as claimed in claim 1, wherein the blue light damage resisting compound is contained in the cosmetic in a concentration of 0.2-1.5% by mass.