CN106726676A - A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy - Google Patents

A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy Download PDF

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Publication number
CN106726676A
CN106726676A CN201710226360.7A CN201710226360A CN106726676A CN 106726676 A CN106726676 A CN 106726676A CN 201710226360 A CN201710226360 A CN 201710226360A CN 106726676 A CN106726676 A CN 106726676A
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hfp
prt
cosmetics
artificial sequences
skin
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顾银凤
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/345—Alcohols containing more than one hydroxy group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/42—Amides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
    • A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/678—Tocopherol, i.e. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/70—Biological properties of the composition as a whole
    • A61K2800/72—Hypo-allergenic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/83—Electrophoresis; Electrodes; Electrolytic phenomena

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  • General Engineering & Computer Science (AREA)
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  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention discloses a kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy, by 0.1 part of pomegranate polypeptide, 2 parts of olive oil, 0.5 part of D panthenols, 0.5 part of vitamin E, 0.1 part of glutathione, 1 part of pyrrolidone sodium carboxylate, 2 parts of glycerine, 1 part of sodium lactate, 0.1 part of essence, 5 parts of purified water compositions.Cosmetic material of the invention is cheap, abundance, natural, gentle, non-stimulated to skin, and various active material acts synergistically on skin, and its effect is better than ordinary cosmetics.

Description

A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
Technical field
The present invention relates to a kind of cosmetics, for the anti-aging of skin, whitening, moisturizing.
Background technology
Skin is to be covered in human body surface ー vitals, by the appendicle of epidermis, corium, hypodermis and skin Official is constituted.Skin is the natural cover for defense together between external environment and body, protects the organ in body from temperature and humidity The influence of change, and external environment (such as ultraviolet and pollutant) influence, however, the protective effect of skin is limited , when external influence exceedes certain degree, skin aging is with regard to Cheng Wei ー kind certainty.
Anti-aging cosmetics have accounted for more than the 50% of Cosmetic Market.One of main factor of skin senescence, is skin The long-term skin photosensitiveness caused by ultraviolet radioactive is aging.The light aging of skin is because free-radical contents increase in skin, freely Base can damage biological film, promote elastomer to crosslink and be polymerized, accelerate skin it is aging.Confrontation and elimination free radical are prevented The damaging action of free radical, realizes often through antioxidant.Current antioxidant is divided into two classes：One is anti-oxidant non-enzyme Agent, such as VE, VC；Another kind of is enzyme antioxidant, wherein most popular is superoxide dismutase.This two classes antioxygen After agent is added in cosmetics, stability of the enzyme antioxidant in cosmetics is poor, and because molecular weight is larger, penetrates into Skin is restricted, therefore its use still has limitation.Therefore, it is more good using small molecule non-enzyme antioxidant.And Vitamin series non-enzyme antioxidant also limit its application in cosmetics due to less stable.Grinding in recent years Study carefully discovery, be a bit Wheat Protein from the Molecular antioxidant substances such as polypeptide of the middle extraction such as plant, Chinese herbal medicine, but Its effect is not also apparent.
Pomegranate is Punicaceae (Punicaceae), Punica, machaka or dungarunga.Pomegranate is nutritious, sugar content Up to 11%~14%, tartaric acid 0.4%~1.0%, moisture content 79%, protein, fat respectively account for 0.6%, ascorbic content It is 2~3 times of apple and pears, is protruded the most with the content of phosphorus especially, per hectogram up to 145 milligrams, is come out at the top in fruit.Pomegranate Juice haematochrome has certain Scavenging activity to DPPH, and its antioxidation is close with Vc, the juice of my pomegranate haematochrome pair of 2.Omg/ml The clearance rate of DPPH shows that juice of my pomegranate haematochrome has good antioxidation activity up to 91.48%.Juice of my pomegranate haematochrome is added to In cosmetic formulations, find after tested：The cosmetics after using 28 days, dispel substantially by epidermis smooth cut, the tight system lifting of skin, Effect of anti-aging is obvious.But, the cosmetics exploitation based on pomegranate is not yet ripe at present, does not there is the utilization of correlation. Have great application prospect.
The content of the invention
Anti-aging cosmetics of the polypeptide as main active are extracted with pomegranate it is an object of the invention to provide a kind of, is utilized The pomegranate polypeptide for extracting is added in cosmetic formulations, is equipped with the cosmetics that other auxiliary materials and component are prepared from.By surveying Trying the cosmetics has functions that good anti-aging.
The anti-aging cosmetics of this invention Ti Chu ー kind external applications, it is characterised in that be made up of each component of following weight portion： 0.1 part of pomegranate polypeptide, 2 parts of olive oil, 0.5 part of D-pantothenyl aleohol, 0.5 part of vitamin E, 0.1 part of glutathione, 2-pyrrolidone-5-carboxylic acid 1 part of sodium, 2 parts of glycerine, 1 part of sodium lactate, 0.1 part of essence, 5 parts of purified waters.
The present invention additionally provides a kind of preparation method of pomegranate polypeptide, (1) cleans up pomegranate, rubs, and squeezes the juice, and adds Papain and trypsase, enzyme concentration 20000IU/g pomegranates, 55 DEG C of hydrolysis temperature, pH value 7.0, enzymolysis time 3h, enzymolysis After the completion of 92 DEG C of enzyme 13min that go out；
(2) material filtering after enzyme that goes out removes insoluble matter, obtains solution, and gained polypeptide solution adds 4% charcoal absorption Decolourize, peptide separation, 20mmol/L HCl solutions wash-out, flow velocity 1.3mL/ are carried out with glucan G-50 (Sephadex G-50) Minute, the eluted product of different time sections is collected respectively, to pH7.0,10000 revs/min are centrifuged 15 minutes regulation solution, through big After the resin DA201-C desalting processings of hole, vacuum concentration, supernatant freeze-drying is standby；Through SDS-polyacrylamide acyl Amine gel electrophoresis (SDS-PAGE), reclaims the band of small-molecular-weight, wherein by functional verification, 60 sequences of small peptide are obtained Function with anti-aging.According to the peak separation time different in chromatographic column, corresponding small peptide can be in batches obtained, also can people The described polypeptide of work synthesis.The sequence of the polypeptide such as SEQ ID NO：Shown in 1-60.
The beneficial effects of the invention are as follows:
Cosmetic material of the invention is cheap, abundance.Pomegranate polypeptide is combined with other active materials, can be more preferably Nourishes rough skin, effectively prevention and to anti-wrinkle generate, increase skin elasticity, anti-aging.The present invention use pomegranate polypeptide for The cosmetics that raw material is prepared, it is natural, gentle, non-stimulated to skin, and also various active material acts synergistically on skin, its Effect is better than ordinary cosmetics.
The detailed description of preferred embodiment
Following example illustrate each aspect of the present invention content.They are only used to illustrate, do not limit model of the invention Enclose.
The preparation of the polypeptide of embodiment 1
(I) pomegranate is cleaned up, is rubbed, squeezed the juice, add papain and trypsase, enzyme concentration 20000IU/g Pomegranate, 55 DEG C of hydrolysis temperature, pH value 7.0, enzymolysis time 3h, 92 DEG C of enzyme 13min that go out after the completion of enzymolysis；
(2) material filtering after enzyme that goes out removes insoluble matter, obtains solution, and gained polypeptide solution adds 4% charcoal absorption Decolourize, peptide separation, 20mmol/L HCl solutions wash-out, flow velocity 1.3mL/ are carried out with glucan G-50 (Sephadex G-50) Minute, the eluted product of different time sections is collected respectively, to pH7.0,10000 revs/min are centrifuged 15 minutes regulation solution, through big After the resin DA201-C desalting processings of hole, vacuum concentration, supernatant freeze-drying is standby；Through SDS-polyacrylamide acyl Amine gel electrophoresis (SDS-PAGE), reclaims the band of small-molecular-weight, wherein by functional verification, 60 sequences of small peptide are obtained Function with anti-aging.The sequence of the polypeptide such as SEQ ID NO：Shown in 1-60, the corresponding entitled HFP-1 of difference~ HFP-60。
The preparation of the cosmetics of embodiment 2
0.1 part of pomegranate polypeptide HFP-1 is taken respectively, is dissolved with 2 parts of 2 parts of glycerine and olive oil.Add successively under agitation Enter 0.5 part of D-pantothenyl aleohol, 0.5 part of vitamin E, 0.1 part of glutathione, 1 part of pyrrolidone sodium carboxylate, 1 part of sodium lactate, essence 0.1 Part, it is sufficiently stirred for making to be completely dissolved, mix, the pH to 5.5~6.5 of solution is adjusted, it is total finally to supply formula with 5 parts of purified waters Amount, aseptic filtration, as packing, finished product cosmetics.
The preparation of cosmetics HFP-2~60 of embodiment 3
According to the method for embodiment 2, pomegranate polypeptide is replaced with into HFP-2~60 totally 59 kinds of forms respectively, remaining component and Preparation method and embodiment 2 are identical, prepare the cosmetics.
Embodiment 4 compares the preparation of cosmetics
According to the method for embodiment 2, wherein be added without corresponding pomegranate polypeptide, remaining component and method and the phase of embodiment 2 Together, corresponding cosmetics are prepared.
The security verification of embodiment 5
Test material and method
Animal：Regular grade experimental animal White Rabbit.Body looks into animal no abnormality seen.
Environmental condition：Room temperature：22 DEG C -23 DEG C, relative humidity：66% -68%；
Test method：
Foundation《Cosmetics health specification》(version in 2007) acute skin irritation is tested, and tests first 24 hours, will be tested Back part of animal backbone diamond wool is cut, 3 centimetres X3 centimetres of unhairing scope.Take and tested be directly applied to right side skin without 0.5 gram of originality On skin, covered with clean gauze, with nonirritant immobilization with adhesive tape 2 hours.Other side is negative control.After removing tested material Observe animal subject contact site dermoreaction and keep a record within 1 hour, 24 hours, 72 hours 48 hours.
Conclusion：
Under this experimental condition, tested material contains the cosmetics of pomegranate polypeptide to White Rabbit acute skin irritation test by skin Skin stimulus intensity is classified as nonirritant.Control cosmetics are also nonirritant.
The cosmetics moisturizing degree of embodiment 6 and moisture preserving time are detected
MMV (moisturemeasurementvalue) method detection MMV values reflect the ability of keratoderma combination water.Adopt The aquation of skin is tested with CM825 type skin oil-water soda acids tester.Its measuring principle is because cuticula contains greatly The electrolyte of amount, the electrolyte existed in water is conductive, and this phenomenon is also produced in skin surface, by test probe with After skin contact, the change of electrical conductivity reflects the hydration status of skin.
Subject is health without dermopathic volunteer.Experimental condition is that the temperature and humidity of environment during testing is constant, Temperature is 25 DEG C ± 2 DEG C, and relative humidity is 40%~60%.
Health is chosen without dermopathic volunteer 62, is tested.Method of testing is：1. the 4 pieces of surveys of left and right forearm are determined Examination region, region area is 4cmX4cm, and the blank value in the region is tested respectively.2. sample is coated on test zone, consumption is 2mg/cm2.3. final test is carried out using rear 120min, 5 points of every piece of domain test are averaged.Moistening effect is evaluated Judgement schematics：With respect in moisturizing rate/%=(P sample t=x-P blank)/(P mark t=x-P blank) X100% formulas：P samples t=x- Use the electrical conductivity of product after certain hour；The electrical conductivity of P blank-be not coated with sample；P mark t=x- use reference after certain hour The electrical conductivity of thing, moisturizing rate situation (%) result that cosmetics of the invention and glycerine compare is as shown in the table：
From in terms of above table, the moisturizing rate of cosmetics of the invention apparently higher than glycerine moisturizing situation, while also illustrating Polypeptide of the invention has significant facilitation for improving the moistening effect of cosmetics.
The senile-resistant efficacy evaluation test of embodiment 6
Cosmetics are detected to the anti-aging effects of skin
Free radical is the intermediate product of biochemical reaction during vital movement, and relevant biology aging with life is freely Base is mainly some oxygen containing free radicals, and antioxidant can provide proton to peroxide radical, it is thus possible to suppress lipid Autoxidizable chain reaction.Generally make DPPH to check antioxidant to put forward protogenic ability.DPPH is a kind of very stable Free radical, its methanol solution has an extremely strong absorbance at 517nm, but when oxidized agent (AH) or free radical (when RO is reduced, Its absorbance can be reduced or disappeared.Thus, can judge whether sample has removing freely using absorbance of the sample at 517nm The ability of base.
Cosmetics are taken, the solution of 100mg cosmetics/ml is configured to PBS, respectively with 3.8ml methanol solutions, Iml (DPPH) radical methanol solutions (2Xl0-4mol/L), is fully mixed evenly, and after standing 30min, is measured with spectrophotometric Absorbance at 517nm, and its free radical scavenging activity is calculated with formula.Free radical scavenging activity (%)=[(Ac-As/Ac)] X100% As:Absorbance of the sample containing determinand at 517nm；Ac:Absorbance of the sample without determinand at 517nm is different The free radical scavenging activity (%) of the Chinese medical extract of volume, its result is following (the unit % of result)：
As can be seen from the table, cosmetics of the invention are higher for free radical scavenging activity.Illustrate cosmetics of the invention With good anti-aging ability.
The face cream whitening effect of embodiment 7 is detected
Each 61 deeper of men and women volunteer of the selection colour of skin, to right face cosmetics of the invention after morning and evening face cleaning, is carried out Sooner or later the smearing of 12h is spaced, left face only smears the cosmetics of embodiment 4, try out surrounding, filled out according to sensory evaluation as control Write evaluation table on probation.4 points of best result is effect is significant, and minimum to be divided into 1, contrast effect is not obvious.Respectively in 4th week using card Fang Jinhang is counted.
Found by detecting, after four weeks has been used, right face is occurred in that significantly the cosmetics containing HFP-1~60 polypeptide Spot fading effect (P<0.05).The effect of data above explanation present invention has whitening.And right face uses the left and right face of glycerine Portion is not significantly different from, and illustrates that cosmetics of the invention have more significant white-skinned face function.
Sequence table
The ＞ of ＜ 110 turn round and look at silver-colored phoenix
A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy of the ＞ of ＜ 120
〈160〉60
〈210〉1
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-1
RFIVHSLPKPSMHPAQRRR
〈210〉2
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-2
PQQCVLVQTTWMSLEGGRS
〈210〉3
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-3
PPRPYYPQQTEFSHDQRK
〈210〉4
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-4
RLLSSDWNQLQEYWRYMV
〈210〉5
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-5
WRHRNWQGQGLGRGDDD
〈210〉6
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-6
DRDMLIAQTMKGTVTS
〈210〉7
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-7
GIFFTETLNSNCTETYP
〈210〉8
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-8
LWAKWWHLIYPHHYIH
〈210〉9
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-9
MKHWLRNEWRIERVMDQQA
〈210〉10
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-10
PDLMYSYPPTIQIIGLNT
〈210〉11
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-11
AMGKMTNFVLSHNPTST
〈210〉12
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-12
TITYSTQLIRRYRSHM
〈210〉13
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-13
DSCVQAIHWMWWDEVLSGY
〈210〉14
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-14
INKPWQQSGHFADSWKRG
〈210〉15
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-15
VMFTDIKCPCQFAHMND
〈210〉16
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-16
LPIPRKTSPWCIWSYN
〈210〉17
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-17
RVSMYWERSDNGFHQRQES
〈210〉18
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-18
LPGDTHAPWWWRPVLIAK
〈210〉19
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-19
AMHGMFSTEGTPQEDAP
〈210〉20
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-20
RSHWHEAWHLVCIGGH
〈210〉21
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-21
IPHQELWQKYSQIFLPWTS
〈210〉22
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-22
RLESKRIPFQRGEPRGPMY
〈210〉23
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-23
EWCHQCWMCGVAQMWGHF
〈210〉24
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-24
PYMAGPPRDREGIHFLH
〈210〉25
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-25
EYEGGWPNEQAPAPKG
〈210〉26
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-26
NPPHRCADHQQEVMVSFH
〈210〉27
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-27
KKFFTEWGVGSQQGAPN
〈210〉28
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-28
LFIHCRVKKRMTCHEW
〈210〉29
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-29
EMIWKGRRTPVTDIARPLV
〈210〉30
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-30
NPWRCRVMRPERCHDINQ
〈210〉31
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-31
IWHMCTKTWRFIDSFEK
〈210〉32
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-32
WVSRWVMFSPHPLPVM
〈210〉33
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-33
MGIRSWWCILMIWQHPRQY
〈210〉34
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-34
IITQYVYPDLWYVQPWGI
〈210〉35
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-35
LSDFTWMNLREGWGGVE
〈210〉36
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-36
VYSHRYCLTWEHNLHA
〈210〉37
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-37
GYHSHGLWHFRWHLIYMR
〈210〉38
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-38
VFRNTWWVEHYDGTSNR
〈210〉39
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-39
LQLPRVPTHMYPSHLN
〈210〉40
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-40
VYDLWTDQQSWDFGRHIPP
〈210〉41
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-41
QYRNPEFQHNSWTSDIKNI
〈210〉42
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-42
WDHARRWHHDYEWPRSCRR
〈210〉43
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-43
KMEMHDDYSYKFICSQQWG
〈210〉44
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-44
GSWLNNPRNSVGDPECFG
〈210〉45
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-45
FKGGHPDQVDFKRLRYQHP
〈210〉46
〈211〉19
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-46
PHNMMLVHLVLHSGNQYGH
〈210〉47
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-47
FCSWQFPDELCYTSISSY
〈210〉48
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-48
IGETRCQQNQFKLLRGG
〈210〉49
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-49
PKPGANIIYERECPPY
〈210〉50
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-50
DQWSWPQEHKLPSRGSQQ
〈210〉51
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-51
NCEGTKRISELICIIKH
〈210〉52
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-52
NTCWSRDHMICFWHVC
〈210〉53
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-53
SSINQHQYGNRAESHLM
〈210〉54
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-54
IWWVLFFPQIYWTGPH
〈210〉55
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-55
EYKSSLHLGRWSWWSMNG
〈210〉56
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-56
TDPFNFNSMTYHRRHCV
〈210〉57
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-57
CHTYPSPLNAYHWFKS
〈210〉58
〈211〉18
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-58
DKQGQILTETKKSSQEQC
〈210〉59
〈211〉17
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-59
WGSVTSRMGHVADPQLH
〈210〉60
〈211〉16
〈212〉PRT
The ＞ artificial sequences of ＜ 213
〈400〉HFP-60
NHCMHFHNCYHPCRCW

Claims (3)

1. a kind of cosmetics, it is characterised in that each component including following weight portion：2 parts of olive oil, 0.5 part of D-pantothenyl aleohol, dimension Raw 0.5 part of element E, 0.1 part of glutathione, 1 part of pyrrolidone sodium carboxylate, 2 parts of glycerine, 1 part of sodium lactate, 0.1 part of essence, 5 parts of purified waters, 0.1 part of pomegranate polypeptide.
2. cosmetics as claimed in claim 1, it is characterised in that described pomegranate polypeptide sequence such as SEQ ID NO：12 institutes Show.
3. a kind of polypeptide, its sequence such as SEQ ID NO：Shown in 12.
CN201710226360.7A 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy Pending CN106726676A (en)

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CN201710226360.7A CN106726676A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201610083775.9A CN105596229B (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy

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CN201710226358.XA Pending CN106726675A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226363.0A Withdrawn CN106821786A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226872.3A Withdrawn CN106880528A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226362.6A Pending CN106806162A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226372.XA Pending CN106806163A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226371.5A Pending CN106726679A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226360.7A Pending CN106726676A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226366.4A Pending CN106880526A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226368.3A Pending CN106880527A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201610083775.9A Active CN105596229B (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226376.8A Withdrawn CN106726680A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226377.2A Withdrawn CN106821787A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226365.XA Withdrawn CN106726677A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226364.5A Withdrawn CN106880525A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201810543087.5A Pending CN108578277A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226369.8A Pending CN106726678A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
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CN201710226358.XA Pending CN106726675A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226363.0A Withdrawn CN106821786A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226872.3A Withdrawn CN106880528A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226362.6A Pending CN106806162A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226372.XA Pending CN106806163A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
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CN201710226366.4A Pending CN106880526A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226368.3A Pending CN106880527A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201610083775.9A Active CN105596229B (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226376.8A Withdrawn CN106726680A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226377.2A Withdrawn CN106821787A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226365.XA Withdrawn CN106726677A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226364.5A Withdrawn CN106880525A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201810543087.5A Pending CN108578277A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226369.8A Pending CN106726678A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy
CN201710226367.9A Pending CN106821804A (en) 2016-02-13 2016-02-13 A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy

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CN106137785B (en) * 2016-08-03 2019-05-17 夏献民 A kind of regenerated skin care item of promotion skin repair
CN112294720A (en) * 2020-11-12 2021-02-02 珂蓝(重庆)化妆品有限公司 Composition for protecting skin from pigment and application thereof
CN117771123A (en) * 2023-11-30 2024-03-29 梅晔生物医药股份有限公司 Moisturizing and seepage-promoting composition, application thereof and daily chemical product

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CN103251524B (en) * 2013-05-06 2015-02-11 广州立白企业集团有限公司 Foam facial mask composition and preparation method thereof
CN103860453B (en) * 2014-04-11 2016-03-09 六安市丹皇生物科技有限责任公司 A kind of face of living is compacted emollient cream and preparation method thereof

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CN106726679A (en) 2017-05-31
CN106880527A (en) 2017-06-23
CN106726677A (en) 2017-05-31
CN106821804A (en) 2017-06-13
CN105596229B (en) 2017-05-31
CN106821786A (en) 2017-06-13
CN106880525A (en) 2017-06-23
CN106806163A (en) 2017-06-09
CN106880528A (en) 2017-06-23
CN106726680A (en) 2017-05-31
CN106821787A (en) 2017-06-13
CN106806162A (en) 2017-06-09
CN106726678A (en) 2017-05-31

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Application publication date: 20170531