CN108578277A

CN108578277A - A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy

Info

Publication number: CN108578277A
Application number: CN201810543087.5A
Authority: CN
Inventors: 顾银凤
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-02-13
Filing date: 2016-02-13
Publication date: 2018-09-28
Also published as: CN106726676A; CN106880528A; CN105596229A; CN106806163A; CN106726677A; CN106880526A; CN106821786A; CN106806162A; CN106880525A; CN106726678A; CN106726675A; CN105596229B; CN106880527A; CN106821787A; CN106726679A; CN106726680A; CN106821804A

Abstract

The invention discloses it is a kind of with anti-aging, whitening, moisture-keeping efficacy cosmetics, by 0.1 part of pomegranate polypeptide, 2 parts of olive oil, 0.5 part of D panthenols, 0.5 part of vitamin E, 0.1 part of glutathione, 1 part of pyrrolidone sodium carboxylate, 2 parts of glycerine, 1 part of sodium lactate, 0.1 part of essence, 5 parts of purified water compositions.The cosmetic material of the present invention is cheap, abundance, natural, mild, non-stimulated to skin, and various active substance acts synergistically on skin, and effect is better than ordinary cosmetics.

Description

A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy

Technical field

The present invention relates to a kind of cosmetics, for the anti-aging of skin, whitening, moisturizing.

Background technology

Skin is to be covered in human body surface ー vitals, by the appendicle of epidermis, corium, subcutaneous tissue and skin Official is constituted.Skin is the natural cover for defense together between external environment and body, protects the organ in body from temperature and humidity The influence of variation and the influence of external environment (such as ultraviolet light and pollutant), however, the protective effect of skin is limited , when external influence is more than certain degree, skin aging is inevitable with regard to Cheng Wei ー kinds.

Anti-aging cosmetics have accounted for 50% of Cosmetic Market or more.One of main factor of skin aging is skin Skin photosensitiveness aging caused by by ultraviolet radioactive for a long time.The light aging of skin is since free-radical contents increase in skin, freely Base can damage biological film, promote elastomer to crosslink and polymerize, accelerate the aging of skin.Confrontation and elimination free radical prevent The damaging action of free radical, is realized often through antioxidant.Current antioxidant is divided into two classes：First, non-enzyme is anti-oxidant Agent, such as VE, VC；Another kind of is enzyme antioxidant, wherein most popular is superoxide dismutase.This two classes antioxygen After agent is added in cosmetics, stability of the enzyme antioxidant in cosmetics is poor, and since molecular weight is larger, penetrates into Skin is restricted, therefore its use still has limitation.Therefore, more good using small molecule non-enzyme antioxidant.And Vitamin series non-enzyme antioxidant also limits its application in cosmetics since stability is poor.Grinding in recent years Study carefully discovery, the Molecular antioxidant substances extracted from plant, Chinese herbal medicine etc. such as polypeptide is Wheat Protein a bit, still Its effect is not also apparent.

Pomegranate is Punicaceae (Punicaceae), Punica, machaka or dungarunga.Pomegranate is full of nutrition, sugar content Up toTartaric acidMoisture content 79%, protein, fat respectively account for 0.6%, and ascorbic content is apple With pearsTimes, it is especially the most prominent with the content of phosphorus, per hectogram up to 145 milligrams, come out at the top in fruit.Juice of my pomegranate is red Element has certain Scavenging activity to DPPH, and antioxidation is close with Vc, and the juice of my pomegranate haematochrome of 2.Omg/ml is to the clear of DPPH Except rate is up to 91.48%, show that juice of my pomegranate haematochrome has good antioxidant activity.Juice of my pomegranate haematochrome is added to cosmetics and matches Fang Zhong has found after tested：The cosmetics after using 28 days, dispel obviously by epidermis smooth cut, and the tight system of skin is promoted, and is slowed down aging The effect of it is apparent.But the cosmetics exploitation at present based on pomegranate is not yet ripe, does not there is relevant utilization.With very big Application prospect.

Invention content

Polypeptide is extracted as the anti-aging cosmetics of main active using pomegranate the object of the present invention is to provide a kind of, is utilized The pomegranate polypeptide extracted is added in cosmetic formulations, is equipped with other auxiliary materials and cosmetics that component is prepared.By surveying Trying the cosmetics has good anti-aging function.

The anti-aging cosmetics of this invention Ti Chu ー kind external applications, which is characterized in that be made of each component of following parts by weight： 0.1 part of pomegranate polypeptide, 2 parts of olive oil, 0.5 part of D-pantothenyl aleohol, 0.5 part of vitamin E, 0.1 part of glutathione, 2-pyrrolidone-5-carboxylic acid 1 part of sodium, 2 parts of glycerine, 1 part of sodium lactate, 0.1 part of essence, 5 parts of purified waters.

The present invention additionally provides a kind of preparation methods of pomegranate polypeptide, and (1) cleans up pomegranate, rub, squeeze the juice, and are added Papain and trypsase, enzyme concentration 20000IU/g pomegranates, 55 DEG C of hydrolysis temperature, pH value 7.0, enzymolysis time 3h, enzymolysis 92 DEG C of enzyme deactivation 13min after the completion；

(2) material filtering after enzyme deactivation removes insoluble matter, obtains solution, and 4% activated carbon adsorption is added in gained polypeptide solution Decoloration carries out peptide separation, the elution of 20mmol/L HCl solutions, flow velocity 1.3mL/ with glucan G-50 (Sephadex G-50) Minute, eluted product in different time periods is collected respectively, adjusts solution to pH7.0,10000 revs/min centrifuge 15 minutes, through big It after the resin DA201-C desalting processings of hole, is concentrated in vacuo, supernatant freeze-drying is spare；Through sodium dodecyl sulfate-polypropylene acyl Amine gel electrophoresis (SDS-PAGE), recycles the band of small-molecular-weight, wherein passing through functional verification, the sequence of 60 small peptides is obtained Has the function of anti-aging.According to the peak separation time different in chromatographic column, corresponding small peptide can be obtained in batches, it also can people The work synthesis polypeptide.The sequence of the polypeptide such as SEQ ID NO：Shown in 1-60.

The beneficial effects of the invention are as follows:

The cosmetic material of the present invention is cheap, abundance.Pomegranate polypeptide is combined with other active materials, can be more preferable Nourishes rough skin, effectively prevent and to anti-wrinkle generate, increase skin elasticity, slow down aging.The present invention use pomegranate polypeptide for The cosmetics that raw material is prepared, it is natural, mild, non-stimulated to skin, and also various active substance acts synergistically on skin, Effect is better than ordinary cosmetics.

The detailed description of preferred embodiment

Following example illustrate each aspect of the present invention contents.They are only to illustrate, and do not limit the model of the present invention It encloses.

The preparation of 1 polypeptide of embodiment

(I) pomegranate is cleaned up, rubs, squeezes the juice, papain and trypsase, enzyme concentration 20000IU/g is added Pomegranate, 55 DEG C of hydrolysis temperature, pH value 7.0, enzymolysis time 3h, 92 DEG C of enzyme deactivation 13min after the completion of enzymolysis；

(2) material filtering after enzyme deactivation removes insoluble matter, obtains solution, and 4% activated carbon adsorption is added in gained polypeptide solution Decoloration carries out peptide separation, the elution of 20mmol/L HCl solutions, flow velocity 1.3mL/ with glucan G-50 (Sephadex G-50) Minute, eluted product in different time periods is collected respectively, adjusts solution to pH7.0,10000 revs/min centrifuge 15 minutes, through big It after the resin DA201-C desalting processings of hole, is concentrated in vacuo, supernatant freeze-drying is spare；Through sodium dodecyl sulfate-polypropylene acyl Amine gel electrophoresis (SDS-PAGE), recycles the band of small-molecular-weight, wherein passing through functional verification, the sequence of 60 small peptides is obtained Has the function of anti-aging.The sequence of the polypeptide such as SEQ ID NO：Shown in 1-60, corresponding entitled HFP-1~ HFP-60。

The preparation of 2 cosmetics of embodiment

HFP-10.1 parts of pomegranate polypeptide is taken respectively, is dissolved with 2 parts of 2 parts of glycerine and olive oil.Add successively under stiring Enter 0.5 part of D-pantothenyl aleohol, 0.5 part of vitamin E, 0.1 part of glutathione, 1 part of pyrrolidone sodium carboxylate, 1 part of sodium lactate, essence 0.1 Part, being sufficiently stirred makes to be completely dissolved, mixing, adjust the pH of solution toFormula total amount finally is supplied with 5 parts of purified waters, Aseptic filtration, packing, as finished product cosmetics.

The preparation of 3 cosmetics HFP-2~60 of embodiment

According to the method for embodiment 2, pomegranate polypeptide is replaced with into HFP-2~60 totally 59 kinds of forms respectively, remaining component and Preparation method and embodiment 2 are identical, and the cosmetics are prepared.

Embodiment 4 compares the preparation of cosmetics

According to the method for embodiment 2, wherein being added without corresponding pomegranate polypeptide, remaining component and method and 2 phase of embodiment Together, corresponding cosmetics are prepared.

5 security verification of embodiment

Test material and method

Animal：Regular grade experimental animal White Rabbit.Body looks into animal no abnormality seen.

Environmental condition：Room temperature：22 DEG C -23 DEG C, relative humidity：66% -68%；

Test method：

Foundation《Cosmetics health specification》(version in 2007) acute skin irritation is tested, and is tested first 24 hours, will be tested Back part of animal backbone diamond wool is cut, 3 centimetres of unhairing range, 3 centimetres of X.0.5 gram of tested no originality is taken directly to be applied to right side skin On skin, covered with clean gauze, with nonirritant immobilization with adhesive tape 2 hours.Other side is negative control.After removing tested material It observes animal subject contact site dermoreaction and keeps a record within 1 hour, 24 hours, 72 hours 48 hours.

Conclusion：

Under this experimental condition, tested material contains the cosmetics of pomegranate polypeptide to White Rabbit acute skin irritation test by skin Skin stimulus intensity is classified as nonirritant.It is also nonirritant to compare cosmetics.

6 cosmetics moisturizing degree of embodiment and moisture preserving time detection

MMV (moisturemeasurementvalue) method detects the ability of MMV values reflection keratoderma combination water.It adopts The aquation of skin is tested with CM825 type skin oil-water soda acid testers.Its measuring principle is since cuticula contains greatly The electrolyte of amount, the electrolyte deposited in water is conductive, and this phenomenon is also generated in skin surface, by test probe with After skin contact, conductivity changes to reflect the hydration status of skin.

Subject is health without dermopathic volunteer.The temperature and humidity of environment is constant during experimental condition is test, Temperature is 25 DEG C ± 2 DEG C, and relative humidity is 40%~60%.

Health is chosen without dermopathic volunteer 62, is tested.Test method is：1. determining the 4 pieces of surveys of left and right forearm Region is tried, region area 4cmX4cm tests the blank value in the region respectively.2. sample is coated on test zone, dosage is 2mg/cm².3. carrying out final test using rear 120min, 5 points of every piece of domain test are averaged.Moistening effect is evaluated Judgement schematics：In opposite moisturizing rate/%=(P sample t=x-P blank)/(P marks t=x-P blank) X100% formulas：P samples t=x- Use the conductivity of product after a certain period of time；P blank-is not coated with the conductivity of sample；P marks t=x- and uses reference after a certain period of time The conductivity of object, the results are shown in table below for the moisturizing rate situation (%) that cosmetics of the invention and glycerine compare：

In terms of above table, the moisturizing rate of cosmetics of the invention is apparently higher than the moisturizing situation of glycerine, while also illustrating The polypeptide of the present invention has significant facilitation for the moistening effect for improving cosmetics.

6 senile-resistant efficacy evaluation test of embodiment

Cosmetics detect the anti-aging effects of skin

Free radical is the intermediate product of biochemical reaction during vital movement, and biology related with life aging is freely Base is mainly some oxygen containing free radicals, and antioxidant can provide proton to peroxide radical, it is thus possible to inhibit lipid Autoxidizable chain reaction.Usually make DPPH to examine antioxidant to put forward protogenic ability.DPPH is a kind of very stable Free radical, methanol solution has an extremely strong absorbance at 517nm, but when by oxidant (AH) or free radical (when RO is restored, Its absorbance can be reduced or be disappeared.It can judge whether sample has removing freely using absorbance of the sample at 517nm as a result, The ability of base.

Take cosmetics, the solution of 100mg cosmetics/ml be configured to PBS, respectively with 3.8ml methanol solutions, Iml (DPPH) radical methanol solutions (2Xl0-4mol/L), is fully mixed evenly, and after standing 30min, is measured with spectrophotometric Absorbance at 517nm, and its free radical scavenging activity is calculated with formula.Free radical scavenging activity (%)=[(Ac-As/Ac)] X100% As:Absorbance of the sample containing determinand at 517nm；Ac:Absorbance of the sample without determinand at 517nm is different The free radical scavenging activity (%) of the Chinese medical extract of volume, result are following (the unit % of result)：

As can be seen from the table, cosmetics of the invention are higher for free radical scavenging activity.Illustrate the cosmetics of the present invention With good anti-aging ability.

7 face cream whitening effect of embodiment detects

Each 61 of the deeper men and women volunteer of the colour of skin is selected, to the cosmetics of the right face present invention, progress after morning and evening face cleaning Sooner or later it is spaced the smearing of 12h, left face as a contrast, only smears the cosmetics of embodiment 4, tries out surrounding, filled out according to sensory evaluation Write evaluation table on probation.Best result 4 divides for significant effect, minimum to be divided into 1, contrast effect unobvious.Respectively card is utilized in 4th week Fang Jinhang is counted.

By detect find, containing HFP-1~60 polypeptide cosmetics after having used four weeks, right face occurs significantly Spot fading effect (P<0.05).Data above illustrates that the present invention has the effect of whitening.And right face uses the left and right face of glycerine Portion is not significantly different, and illustrates that the cosmetics of the present invention have more significant white-skinned face function.

Sequence table

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<120>A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy

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Arg Phe Ile Val His Ser Leu Pro Lys Pro Ser Met His Pro Ala Gln

1 5 10 15

Arg Arg Arg

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Pro Gln Gln Cys Val Leu Val Gln Thr Thr Trp Met Ser Leu Glu Gly

1 5 10 15

Gly Arg Ser

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Pro Pro Arg Pro Tyr Tyr Pro Gln Gln Thr Glu Phe Ser His Asp Gln

1 5 10 15

Arg Lys

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Arg Leu Leu Ser Ser Asp Trp Asn Gln Leu Gln Glu Tyr Trp Arg Tyr

1 5 10 15

Met Val

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<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

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Trp Arg His Arg Asn Trp Gln Gly Gln Gly Leu Gly Arg Gly Asp Asp

1 5 10 15

Asp

<210> 6

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Asp Arg Asp Met Leu Ile Ala Gln Thr Met Lys Gly Thr Val Thr Ser

1 5 10 15

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<211> 17

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<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

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Gly Ile Phe Phe Thr Glu Thr Leu Asn Ser Asn Cys Thr Glu Thr Tyr

1 5 10 15

Pro

<210> 8

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Leu Trp Ala Lys Trp Trp His Leu Ile Tyr Pro His His Tyr Ile His

1 5 10 15

<210> 9

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 9

Met Lys His Trp Leu Arg Asn Glu Trp Arg Ile Glu Arg Val Met Asp

1 5 10 15

Gln Gln Ala

<210> 10

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 10

Pro Asp Leu Met Tyr Ser Tyr Pro Pro Thr Ile Gln Ile Ile Gly Leu

1 5 10 15

Asn Thr

<210> 11

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 11

Ala Met Gly Lys Met Thr Asn Phe Val Leu Ser His Asn Pro Thr Ser

1 5 10 15

Thr

<210> 12

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 12

Thr Ile Thr Tyr Ser Thr Gln Leu Ile Arg Arg Tyr Arg Ser His Met

1 5 10 15

<210> 13

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 13

Asp Ser Cys Val Gln Ala Ile His Trp Met Trp Trp Asp Glu Val Leu

1 5 10 15

Ser Gly Tyr

<210> 14

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 14

Ile Asn Lys Pro Trp Gln Gln Ser Gly His Phe Ala Asp Ser Trp Lys

1 5 10 15

Arg Gly

<210> 15

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 15

Val Met Phe Thr Asp Ile Lys Cys Pro Cys Gln Phe Ala His Met Asn

1 5 10 15

Asp

<210> 16

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 16

Leu Pro Ile Pro Arg Lys Thr Ser Pro Trp Cys Ile Trp Ser Tyr Asn

1 5 10 15

<210> 17

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 17

Arg Val Ser Met Tyr Trp Glu Arg Ser Asp Asn Gly Phe His Gln Arg

1 5 10 15

Gln Glu Ser

<210> 18

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 18

Leu Pro Gly Asp Thr His Ala Pro Trp Trp Trp Arg Pro Val Leu Ile

1 5 10 15

Ala Lys

<210> 19

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 19

Ala Met His Gly Met Phe Ser Thr Glu Gly Thr Pro Gln Glu Asp Ala

1 5 10 15

Pro

<210> 20

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 20

Arg Ser His Trp His Glu Ala Trp His Leu Val Cys Ile Gly Gly His

1 5 10 15

<210> 21

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 21

Ile Pro His Gln Glu Leu Trp Gln Lys Tyr Ser Gln Ile Phe Leu Pro

1 5 10 15

Trp Thr Ser

<210> 22

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 22

Arg Leu Glu Ser Lys Arg Ile Pro Phe Gln Arg Gly Glu Pro Arg Gly

1 5 10 15

Pro Met Tyr

<210> 23

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 23

Glu Trp Cys His Gln Cys Trp Met Cys Gly Val Ala Gln Met Trp Gly

1 5 10 15

His Phe

<210> 24

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 24

Pro Tyr Met Ala Gly Pro Pro Arg Asp Arg Glu Gly Ile His Phe Leu

1 5 10 15

His

<210> 25

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 25

Glu Tyr Glu Gly Gly Trp Pro Asn Glu Gln Ala Pro Ala Pro Lys Gly

1 5 10 15

<210> 26

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 26

Asn Pro Pro His Arg Cys Ala Asp His Gln Gln Glu Val Met Val Ser

1 5 10 15

Phe His

<210> 27

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 27

Lys Lys Phe Phe Thr Glu Trp Gly Val Gly Ser Gln Gln Gly Ala Pro

1 5 10 15

Asn

<210> 28

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 28

Leu Phe Ile His Cys Arg Val Lys Lys Arg Met Thr Cys His Glu Trp

1 5 10 15

<210> 29

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 29

Glu Met Ile Trp Lys Gly Arg Arg Thr Pro Val Thr Asp Ile Ala Arg

1 5 10 15

Pro Leu Val

<210> 30

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 30

Asn Pro Trp Arg Cys Arg Val Met Arg Pro Glu Arg Cys His Asp Ile

1 5 10 15

Asn Gln

<210> 31

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 31

Ile Trp His Met Cys Thr Lys Thr Trp Arg Phe Ile Asp Ser Phe Glu

1 5 10 15

Lys

<210> 32

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 32

Trp Val Ser Arg Trp Val Met Phe Ser Pro His Pro Leu Pro Val Met

1 5 10 15

<210> 33

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 33

Met Gly Ile Arg Ser Trp Trp Cys Ile Leu Met Ile Trp Gln His Pro

1 5 10 15

Arg Gln Tyr

<210> 34

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 34

Ile Ile Thr Gln Tyr Val Tyr Pro Asp Leu Trp Tyr Val Gln Pro Trp

1 5 10 15

Gly Ile

<210> 35

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 35

Leu Ser Asp Phe Thr Trp Met Asn Leu Arg Glu Gly Trp Gly Gly Val

1 5 10 15

Glu

<210> 36

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 36

Val Tyr Ser His Arg Tyr Cys Leu Thr Trp Glu His Asn Leu His Ala

1 5 10 15

<210> 37

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 37

Gly Tyr His Ser His Gly Leu Trp His Phe Arg Trp His Leu Ile Tyr

1 5 10 15

Met Arg

<210> 38

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 38

Val Phe Arg Asn Thr Trp Trp Val Glu His Tyr Asp Gly Thr Ser Asn

1 5 10 15

Arg

<210> 39

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 39

Leu Gln Leu Pro Arg Val Pro Thr His Met Tyr Pro Ser His Leu Asn

1 5 10 15

<210> 40

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 40

Val Tyr Asp Leu Trp Thr Asp Gln Gln Ser Trp Asp Phe Gly Arg His

1 5 10 15

Ile Pro Pro

<210> 41

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 41

Gln Tyr Arg Asn Pro Glu Phe Gln His Asn Ser Trp Thr Ser Asp Ile

1 5 10 15

Lys Asn Ile

<210> 42

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 42

Trp Asp His Ala Arg Arg Trp His His Asp Tyr Glu Trp Pro Arg Ser

1 5 10 15

Cys Arg Arg

<210> 43

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 43

Lys Met Glu Met His Asp Asp Tyr Ser Tyr Lys Phe Ile Cys Ser Gln

1 5 10 15

Gln Trp Gly

<210> 44

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 44

Gly Ser Trp Leu Asn Asn Pro Arg Asn Ser Val Gly Asp Pro Glu Cys

1 5 10 15

Phe Gly

<210> 45

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 45

Phe Lys Gly Gly His Pro Asp Gln Val Asp Phe Lys Arg Leu Arg Tyr

1 5 10 15

Gln His Pro

<210> 46

<211> 19

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 46

Pro His Asn Met Met Leu Val His Leu Val Leu His Ser Gly Asn Gln

1 5 10 15

Tyr Gly His

<210> 47

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 47

Phe Cys Ser Trp Gln Phe Pro Asp Glu Leu Cys Tyr Thr Ser Ile Ser

1 5 10 15

Ser Tyr

<210> 48

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 48

Ile Gly Glu Thr Arg Cys Gln Gln Asn Gln Phe Lys Leu Leu Arg Gly

1 5 10 15

Gly

<210> 49

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 49

Pro Lys Pro Gly Ala Asn Ile Ile Tyr Glu Arg Glu Cys Pro Pro Tyr

1 5 10 15

<210> 50

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 50

Asp Gln Trp Ser Trp Pro Gln Glu His Lys Leu Pro Ser Arg Gly Ser

1 5 10 15

Gln Gln

<210> 51

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 51

Asn Cys Glu Gly Thr Lys Arg Ile Ser Glu Leu Ile Cys Ile Ile Lys

1 5 10 15

His

<210> 52

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 52

Asn Thr Cys Trp Ser Arg Asp His Met Ile Cys Phe Trp His Val Cys

1 5 10 15

<210> 53

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 53

Ser Ser Ile Asn Gln His Gln Tyr Gly Asn Arg Ala Glu Ser His Leu

1 5 10 15

Met

<210> 54

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 54

Ile Trp Trp Val Leu Phe Phe Pro Gln Ile Tyr Trp Thr Gly Pro His

1 5 10 15

<210> 55

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 55

Glu Tyr Lys Ser Ser Leu His Leu Gly Arg Trp Ser Trp Trp Ser Met

1 5 10 15

Asn Gly

<210> 56

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 56

Thr Asp Pro Phe Asn Phe Asn Ser Met Thr Tyr His Arg Arg His Cys

1 5 10 15

Val

<210> 57

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 57

Cys His Thr Tyr Pro Ser Pro Leu Asn Ala Tyr His Trp Phe Lys Ser

1 5 10 15

<210> 58

<211> 18

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 58

Asp Lys Gln Gly Gln Ile Leu Thr Glu Thr Lys Lys Ser Ser Gln Glu

1 5 10 15

Gln Cys

<210> 59

<211> 17

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 59

Trp Gly Ser Val Thr Ser Arg Met Gly His Val Ala Asp Pro Gln Leu

1 5 10 15

His

<210> 60

<211> 16

<212> PRT

<213>Artificial sequence (2 Ambystoma laterale x Ambystoma jeffersonianum)

<400> 60

Asn His Cys Met His Phe His Asn Cys Tyr His Pro Cys Arg Cys Trp

1 5 10 15

Claims

1. a kind of cosmetics, which is characterized in that include each component of following parts by weight：2 parts of olive oil, 0.5 part of D-pantothenyl aleohol, dimension life 0.5 part of plain E, 0.1 part of glutathione, 1 part of pyrrolidone sodium carboxylate, 2 parts of glycerine, 1 part of sodium lactate, 0.1 part of essence, 5 parts of purifying Water, 0.1 part of pomegranate polypeptide.

2. cosmetics as described in claim 1, which is characterized in that the pomegranate polypeptide sequence such as SEQ ID NO：17-60 Shown in any.

3. a kind of polypeptide, sequence such as SEQ ID NO：Shown in 17-60 is any.