KR20060031329A - A COMPOSITION CONTAINING beta-1,3-GLUCANE AND DEEP SEA WATER FOR ENHANCING SKIN ELASTICITY - Google Patents

Abstract

The present invention relates to a composition for enhancing skin elasticity, wherein the composition contains 0.00001 to 30% by weight of beta-1,3-glucan and 0.001 to 95% by weight of deep sea water as the active ingredient. It is done. Since the composition has a beta-1,3-glucan and deep sea water together to promote collagen biosynthesis and improve skin elasticity, the composition may be used in cosmetics as a composition for promoting skin elasticity.

Beta-1,3-glucan, deep sea water, collagen, skin elasticity, composition, cosmetics

Description

A composition containing β-1,3-glucane and Deep sea water for enhancing skin elasticity}

The present invention relates to a composition for enhancing skin elasticity containing beta-1,3-glucan and deep sea water.

The skin is the body's primary barrier, protecting the organs of the body from stimuli of the external environment, such as changes in temperature and humidity, ultraviolet rays, and pollutants. However, internally, as hormones age, the secretion of various hormones that regulate metabolism decreases, and the function and activity of immune cells decreases, resulting in decreased biosynthesis of immune proteins and bioconstituent proteins necessary for living organisms. As the environmental pollution, such as increased ultraviolet rays of sunlight reaching the skin due to destruction of the ozone layer, becomes more severe, the normal functioning of the skin is caused by excessive physical and chemical irritation and stress caused by the increase of free radicals and active harmful oxygen. Decreases and accelerates the aging of the skin to damage the skin.                         

In order to prevent this phenomenon and maintain healthy and beautiful skin, by using physiologically active substances obtained from various animals, plants, and microorganisms in the past, cosmetics are used to maintain the unique function of the skin and to activate skin cells. Efforts have been made to curb effectively.

However, existing raw materials have various problems such as insufficient efficacy or cause skin side effects. Therefore, the present inventors are studying the substances that can inhibit phenomena such as the reduction of skin cells, the decrease in collagen synthesis, and the like, which are the main indicators of skin aging, and the proliferation and skin of beta-1,3-glucan-containing skin cells. Patent application has been filed for an external preparation composition having an excellent effect on the healing of damage (Korean Patent Application No. 98-49026).

Deep sea water refers to seawater with a depth of 200 m or less from the surface of the sea and does not produce organic matter due to photosynthesis. On the basis of It is known that the deep sea water contains about 60 kinds of mineral components, and many researches such as a method of preparing food using the same are being conducted.

The characteristics of the deep sea water are that the surface water acts as a photosynthesis, which consumes nitrogen, phosphorus, and nitric acid in the water. However, since deep water below 200 m does not reach sunlight, phytoplankton consumes nutrients, and photosynthesis does not occur. This rarely happens. For this reason, inorganic nutrients such as nitrogen, phosphorus, and nitric acid consumed by photosynthesis remain in the depth procedure. In addition, deep sea water contains a lot of minerals and essential trace elements necessary for the human body, which can help supplement minerals that are likely to be insufficient. In addition, the concentration of organic matter is less than 200 m from the surface of the sea water, there is no contamination by E. coli or general bacteria, and there is little possibility of contamination by chemicals from the land or the atmosphere. Or, the temperature is low throughout the year, the change is low and has a stable low water temperature, and because it is water formed for thousands of years, its properties are stable, and it has ripening due to the action of various enzymes. In addition, because it contains essential trace elements and various mineral components in balance, it is known to have excellent scavenging effect on active oxygen due to the action of dissolved metal ions. Effects such as activation are known.

The inventors of the present invention have been researching to further increase the effect of beta-1,3-glucan on the proliferation of skin cells and healing of skin damage. When used together, the present invention finds that this combination composition greatly improves skin elasticity.

Accordingly, it is an object of the present invention to provide a composition for skin elasticity promotion.

In order to achieve the above object, the composition for enhancing skin elasticity of the present invention is characterized in that it contains beta-1,3-glucan and deep sea water.

At this time, the composition for promoting skin elasticity of the present invention is characterized by containing beta-1,3-glucan 0.00001 ~ 30% by weight, deep sea water 0.001 ~ 95% by weight based on the total weight of the composition as an active ingredient.

This is because the content of beta-1,3-glucan is difficult to obtain the expected effect when the content of the composition is less than 0.00001% by weight relative to the total weight of the composition, and the formulation stability is more than 30% by weight. In addition, when the content of the deep sea water is less than 0.001% by weight relative to the total weight of the composition, it is difficult to obtain the expected effect, and when it exceeds 95% by weight, the formulation stability is difficult.

The beta-1,3-glucan of the present invention is characterized in that it is derived from one selected from the group consisting of chima mushrooms, ganoderma lucidum mushrooms, spiritual mushrooms, shiitake mushrooms, mycobacterium bacteria, yeast or barley.

In addition, preferably the beta-1,3-glucan is characterized in that it is derived from Schizopyllum commune , more preferably the beta-1,3-glucan is a purity obtained by liquid culture of the mushroom mushroom mycelium It is characterized by a high purity beta-1,3-glucan of 98% or more.

Deep sea water used in the present invention is characterized in that the dewatering treatment by taking in less than 200 m.

In addition, during the desalting treatment, desalination may be carried out by electrolysis, or desalination may be carried out by using a cationic ion exchange resin or an anionic ion exchange resin, and preferably, desalination by electrolysis.

In addition, the composition for enhancing skin elasticity containing the beta-1,3-glucan and deep sea water of the present invention is a cosmetic composition for enhancing skin elasticity.

In order to investigate the skin elasticity enhancing effect of the beta-1,3-glucan and deep sea water mixture, first, the human skin of beta-1,3-glucan, deep sea water, beta-1,3-glucan and deep sea water mixture After confirming the collagen biosynthesis efficacy on the cells in vitro, each of these skin elasticity enhancing effects were examined in vivo, ie, animal experiments and clinical trials. -When glucan and deep sea water were used together, collagen biosynthesis effect was greatly increased and skin elasticity improvement effect was also higher than when used alone or not.

Accordingly, the present invention provides a cosmetic composition for enhancing skin elasticity containing such beta-1,3-glucan and deep sea water.

In addition, the cosmetic composition for enhancing skin elasticity according to the present invention, in addition to the above-described beta-1,3-glucan and deep sea water, preferably synergistic effect on the main effect within the range that does not impair the main effect of the present invention. It may also contain other elasticity enhancing ingredients that can give. Collagen etc. are mentioned as such a component.

Cosmetic composition containing beta-1,3-glucan and deep sea water of the present invention can be used in a variety of cosmetics, face wash, etc. for skin elasticity promoting effect. Examples of products to which the present composition can be added include cosmetics such as various creams, gels, lotions, and skins, and cleansing agents, face washes, soaps, and the like.                     

In addition, the cosmetic composition for skin elasticity enhancement containing beta-1,3-glucan and deep sea water of the present invention may take the form of solutions, emulsions, viscous mixtures and the like.

The cosmetic composition for enhancing skin elasticity according to the present invention is not particularly limited in the formulation, but for example, latex, cream, lotion, essence, pack, gel, powder, makeup base, foundation, lotion, ointment, gel, patch Formulations such as, cosmetic liquids, cleansing foams, cleansing creams, cleansing water, soaps or sprays and the like.

In the cosmetic composition of each formulation, in addition to the above-described beta-1,3-glucan and deep sea water, other components can be appropriately selected and blended by those skilled in the art according to the formulation or use purpose of other cosmetics.

Such formulations may contain skin absorption promoting substances to increase skin elasticity enhancing effect.

In addition, the cosmetic of the present invention may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.                     

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.001-10 weight% with respect to a total weight, More preferably, it is mix | blended in 0.001-5 weight%.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but the present invention is not limited only to these examples.

Example 1 and Comparative Examples 1 to 3

Example 1 and Comparative Example 1 having the composition of Table 1 below containing beta-1,3-glucan (trade name: SC-Glucane, manufactured by Pacific, Korea) and deep ocean water (trade name: Hawaii ocean deep water, manufactured by Enzamin, USA) A latex type cream formulation of 3 to 3 was used in the following experimental example. At this time, the oil phase and the water phase were completely dissolved at 65 to 75 ° C., and emulsified at 7,000 to 8,000 rpm for 5 minutes to prepare the cream formulation.

In the present embodiment and the comparative example, the cream formulation was used, but the present invention is not limited thereto, and in the case of the agonist, it may be applied to various cosmetic formulations such as gels, lotions, and skins.

On the other hand, Example 1 includes both the beta-1,3-glucan and the deep sea water, Comparative Example 1 does not contain any agonist, Comparative Example 2 includes only beta-1,3-glucan, comparison Example 3 was prepared to include only deep ocean water.

Ingredient Example 1 (% by weight) Comparative Example 1 (% by weight) Comparative Example 2 (wt%) Comparative Example 3 (wt%) Purified water Up to 100 Up to 100 Up to 100 Up to 100 Beta-1,3-glucan 0.1 - 0.1 - Deep ocean water 5.0 - - 5.0 Vegetable Cured Oil 1.50 1.50 1.50 1.50 Glycerol Stearate 0.60 0.60 0.60 0.60 Cetheanyl alcohol 2.00 2.00 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium 1.00 1.00 1.00 1.00 Arachidil Behenyl Alcohol 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 2.00 PEG-100 stearate & glycerol oleate & propylene glycol 1.50 1.50 1.50 1.50 Caprylic / Carlic Triglycerides 11.00 11.00 11.00 11.00 Cyclomedicon 6.00 6.00 6.00 6.00 Preservative, incense Quantity Quantity Quantity Quantity Trimethanol amine 0.10 0.10 0.10 0.10

Experimental Example 1 Collagen Biosynthesis Efficacy on Human Skin Cells

The following experiments were performed to determine the collagen biosynthesis efficacy of human skin cells of beta-1,3-glucan, deep seawater, beta-1,3-glucan and deep seawater mixture.

First, human fibroblasts were cultured in a 24-well plate incubator, and then each sample (test substance) having the concentrations shown in Table 2, namely beta-1,3-glucan, deep sea water, beta-1,3-glucan, and Replace with a medium containing a deep seawater mixture (weight ratio 1: 1) and incubate for 3 days, add 0.5 ml of DMEM medium containing 10% fetal calf serum per well, and then add L [2, 3, 4, 5- 10 μCi of 3H] -proline was added. After 24 hours, the media and cells in each well were scraped and washed in 5% Trichloroacetic acid (TCA) solution and dispensed into two test tubes each and type I in one test tube. 1 unit / μl collagenase (type I collagenase) was added and incubated at 37 ° C. for 90 minutes, and other test tubes were stored at 4 ° C. Thereafter, 0.05 ml of 50% trichloroacetic acid was added to all test tubes, and the mixture was allowed to stand at 4 ° C. for 20 minutes, followed by centrifugation at 12,000 rpm for 10 minutes, respectively. The supernatants and precipitates were separated by liquid scintillation counters (LSC; The liquid scintillation counter (CPM) counts per minute (CPM) values were obtained, and collagen biosynthesis values (RCB; Relative Collagen Biosynthesis) were obtained for the control group and the experimental group based on Equation 1 below. The results were obtained by comparing the control with 100 as shown in Table 2 below.

As a result, as can be seen in Table 2, beta-1,3-glucan, deep seawater, beta-1,3-glucan, and deep seawater mixture increased concentration-dependently collagen biosynthesis of human skin cells, respectively. In particular, beta-1,3-glucan and deep seawater mixtures most increased collagen biosynthesis of human skin cells.

Through this, it was found that the collagen biosynthesis was excellently increased by synergistic effect when the deep ocean water was mixed with beta-1,3-glucan.

Test substance Concentration (ppm) Collagen Biosynthesis Rate (RCB) (%) Beta-1,3-glucan 10 120 One 105 Deep ocean water 10 106 One 102  Beta-1,3-Glucan + Deep Sea Water (Weight 1: 1) 20 140 2 115 Control - 100

Experimental Example 2 Collagen Biosynthesis Efficacy in Animals

In order to confirm the effect of enhancing the collagen biosynthesis efficacy of the beta-1,3-glucan and the deep sea water mixture, after applying the cream formulation having the composition of Example 1 and Comparative Examples 1 to 3 on the back of the hairless mice for a week Biopsy and collagen immunohistostaining were performed. The result was calculated | required by the comparative value which made Comparative Example 1 (control group) which is an untreated group as 100, and is shown in following Table 3.

As can be seen in Table 3, it was observed that the collagen biosynthesis increased by 24% and 5%, respectively, in the groups coated with Comparative Examples 2 to 3 than the group coated with Comparative Example 1. Moreover, when Example 1 containing the beta-1,3-glucan and the deep seawater mixture according to the present invention was applied, collagen biosynthesis was greatly increased to about 49%.

Through this, it was confirmed that the synergistic effect of the beta-1,3-glucan mixed with deep sea water according to the present invention, the effect is much increased.

Test substance Collagen Biosynthesis (%) Example 1 149 Comparative Example 2 124 Comparative Example 3 105 Comparative Example 1 (Control) 100

Experimental Example 3. Improvement of Skin Elasticity in Human Body

In order to determine the effect of improving the skin elasticity of the beta-1,3-glucan and the deep sea water mixture in the human body, the following experiments to improve the skin elasticity of the emulsion-type cream prepared in Example 1 and Comparative Examples 1 to 3 Measured through.

First, 20 healthy women aged 30 years or older at a temperature of 24 to 26 ° C. and a humidity of 75% were divided into two groups, and the facial lotion of Example 1 and Comparative Examples 1 to 3 was applied twice daily to the face of each group of women. After weekly application, skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). The results were obtained from R8 [R8 (left) -R8 (right)] of a skin elasticity analyzer (Cutometer SEM 575) and are shown in Table 4 below. At this time, the R8 value indicates the nature of the skin viscoelasticity. Separately, at the end of the test, the subjects were asked to complete the questionnaire, and the subjective efficacy evaluation was conducted simultaneously with the mechanical evaluation. The results are shown in Table 5 below.

As can be seen in Table 4, when the material of Example 1 containing a mixture of beta-1,3-glucan and deep sea water according to the present invention is applied, compared with the case of applying Comparative Examples 2 to 3 Skin elasticity was further increased.

In addition, through the survey, when applying Example 1 containing a mixture of beta-1,3-glucan and deep sea water according to the present invention, the skin elasticity is significantly improved compared to the case of applying Comparative Examples 2 to 3 Confirmed.

Through this, it was found that when the beta-1,3-glucan was mixed with the deep sea water according to the present invention, the synergistic effect of these was excellent in improving skin elasticity.

Test substance Skin elasticity effect Example 1 0.54 Comparative Example 2 0.27 Comparative Example 3 0.11 Comparative Example 1 (Control) 0.08

Applicator Responders (People) Very good Good usually Inadequate Example 1 3 6 One 0 Comparative Example 2 One 3 5 One Comparative Example 3 One 2 5 2 Comparative Example 1 (Control) One 2 3 4

It contains beta-1,3-glucan and deep sea water of the present invention, characterized by containing beta-1,3-glucan 0.00001 ~ 30% by weight, deep sea water in 0.001 ~ 95% by weight relative to the total weight of the composition The composition for enhancing skin elasticity, by containing beta-1,3-glucan and deep sea water together, promotes the biosynthesis of collagen fibers to improve skin elasticity, and thus can be used in cosmetics as a composition for enhancing skin elasticity. .

Claims (6)

A composition for enhancing skin elasticity, comprising beta-1,3-glucan in an amount of 0.00001 to 30% by weight and deep sea water in an amount of 0.001 to 95% by weight based on the total weight of the composition as an active ingredient. According to claim 1, wherein the beta-1,3-glucan is a skin elasticity composition, characterized in that derived from the one selected from the group consisting of skirt mushroom, Ganoderma lucidum mushroom, shiitake mushroom, shiitake mushroom, fungi, barley . The composition of claim 2, wherein the beta-1,3-glucan is derived from a Schizopyllum commune . The composition for enhancing skin elasticity of claim 3, wherein the beta-1,3-glucan is a high purity beta-1,3-glucan having a purity of 98% or more obtained by liquid culturing the Skirt mushroom mycelium. The composition for enhancing skin elasticity according to claim 1, wherein the deep ocean water is obtained by taking water under 200 m and desalting. According to claim 1, wherein the composition is a composition for promoting skin elasticity is a cosmetic composition.