KR101064410B1 - Composition for cosmetics comprising sparassis crispa extract - Google Patents

Abstract

The present invention relates to a composition having an antioxidant activity, including a mushroom extract, the composition having an antioxidant effect of the present invention comprising a hydrangea hydrothermal extract as an active ingredient not only has excellent DPPH scavenging activity and superoxide anion radical scavenging ability Linoleic acid peroxidation inhibition rate is also excellent and the total phenolic content is high and has excellent antioxidant efficacy, it is expected that it can be useful in various fields, for example, pharmaceutical, food, cosmetics.

Description

Cosmetic composition containing an extract of zinnia mushrooms {COMPOSITION FOR COSMETICS COMPRISING SPARASSIS CRISPA EXTRACT}

The present invention relates to a cosmetic composition comprising a mushroom extract, cosmetic composition of the present invention has an excellent antioxidant effect and excellent cell protection, whitening, anti-wrinkle effect cosmetic composition for skin improvement, skin protection and skin aging prevention It can be usefully used.

Skin is a type of protective shield that protects the body from environmental, physical, and chemical external stimuli and has a very important function for life support. However, these external stimuli not only cause pigmentary diseases such as blemishes and blemishes, but also promote aging of the skin by destroying fibers that lower skin function and maintain skin elasticity. Therefore, efforts to maintain healthy and beautiful skin while preventing such skin phenomena continue. Recently, research efforts to improve skin have been actively studied for developing materials using plant extracts that have no side effects, are friendly to human beings, and can be used safely regardless of constitution.

In general, functional cosmetics containing plant extracts are prepared by extracting plants that grow under conditions suitable for plant growth with various solvents. Plants used here are also various, research on the cosmetic composition using the mushroom extract is also actively in progress.

Mushrooms have been used not only for food but also for medicine since ancient times. There are an estimated 140,000 mushroom species on the planet, approximately 10% known. These mushrooms decompose organic matter for survival, thereby having environmentally friendly properties.

Among the above mushrooms, in particular, the mushroom mushroom (Sparassis crispa) mushroom is rare in nature and is widely known in Japan and Korea recently. The blossom mushroom is named Culiflower mushroom in English, Hanabiratake in Japanese, and blossom in Korean. Blossom mushrooms are the best example of edible by-product mushrooms, and sawdust acts as a substrate and can be used immediately (David Pilz., Et al. R. Molina, 2002].

The NMR structure of the polysaccharides of the Sparassis crispa was found to be 6-branched β- (1-> 3) -D-glucan at 4.78 ppm and 4.55 ppm for β- (1-> 6) -D-glucan. Turned out to be. Flowering mushrooms (Sparassis crispa) have been reported to exhibit β-glucans in water-soluble contents that serve as good dietary fiber. Mushrooms with immunomodulatory polysaccharides can be used in various regions as food as well as health food supplements (functional foods) and drugs.

The clinical trial is a report on Sparassis crispa powder for cancer patients. About 300 mg of S. mushrooms (Sparassis crispa) was given orally to several cancer patients after 1 course of lymphocyte transduction immunotherapy, a total of 14 patients were observed, and 9 months later, 9 patients improved. Ono et al., 2003]. Since the polysaccharides of mushrooms have been reported to exhibit immunomodulatory properties, antitumor activity and anticancer activity, incorporating mushroom extracts into foods is of great benefit.

In 2007, Rui Tada et al. Reported the structure of β-glucans from the Sparassis crispa fruiting bodies by NMR. Purified β-D-glucan exhibits biological activity including antitumor effect, increased hematopoietic response in cyclophosphamide-induced leukopenia mice, and induction of cytokine production, and the main chain structural unit, Sparassis Purification of crispa) The primary structure of β-glucan was found to be β- (1-> 3) -D-glucan with one β- (1-> 6) -D-glucosyl side chain branching unit every three residues. These structural units were identified using NMR spectroscopy as well as formic acid digestion and zymolyase digestion. As a result, Spartassis crispa beta glucan can be applied to food therapy.

Oral administration of the medicinal mushroom Sparassis crispa has wound healing properties in streptozotocin induced diabetic rats. It was found that diabetes induced rats had increased macrophage and fibroblast migration, collagen regeneration and epithelial formation. In dermal fibroblasts, type I collagen was increased by Sparassis crispa beta glucan, and significant concentration was observed above 100 µg / ml. [Hon Kwon et al., 2009]. Many studies and investigations relate to the study of wound and wound healing systems that benefit humans. In the case of diabetics, damaged wound healing is a major clinical challenge. Glucan is a glucose polymer and has an immune stimulating activity that enhances the wound healing process. 1,3-β-glucan, a polysaccharide fraction found in the mushroom (Sparassis crispa), has been shown to have strong antitumor activity that inhibits sarcoma solid form in mice [Ono et al., 2000]. Harada et al. Reported that, in 2002 and 2000, Spartassis crispa helped to increase hematopoietic response and high induction of interferon-γ in splenocytes in cyclophosphamide-induced leukopenia mice. It was.

To date, there has been only one preliminary report on the proteomics analysis of Spartassis crispa (Horie). Rakwal et al., 2008]. The authors report the proteomic analysis of the Sparris crispa fruiting body using a complementary proteomics approach based on 1- and 2-dimensional gel electrophoresis (1-DGE and 2-DGE). 1-DGE linked to liquid chromatography and mass spectrophotometry revealed 77 (60 non-redundant proteins) proteins from Sparassis crispa. 2-DGE analysis revealed 480 protein spots stained by Coomassie brilliant blue in the Sparassis crispa.

Among 71 select protein spots from Sparassis crispa 2D gel blots on polyvinyldifluoride (PVDF) membranes, 29 non-excess proteins were identified by N-terminal amino acid sequencing. Non-excess proteins identified from 1- or 2-DGE belonged to 19 functional categories. Twelve proteins, including 14-3-3 protein and septin, were found in Spartassis crispa. The study provides evidence for the presence of many functionally diverse proteins expressed in the fruiting body of the Sparassis crispa, an economically important mushroom. Proteomic data obtained from the 1-DGE and 2-DGE assays can be obtained via the mushroom proteomics portal http://foodfunc.agr.ibaraki.ac.jp/mushprot.html .

Due to the potential use of these blossom mushrooms in a variety of diseases related to humans, there is an in-depth study of novel components from them in the art. However, there have been no reports about the antioxidant effect of the zinnia mushroom. Accordingly, the present inventors found that the mushroom extract has an antioxidant effect while studying the blossom mushroom in more depth, and completed the present invention as a result of repeated studies to further improve the blossom mushroom extract.

Published Patent Publication 10-2010-0067431

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An object of the present invention is to provide a cosmetic composition having an antioxidant activity, including the mushroom extract.

In order to achieve the above object, the present invention provides a cosmetic composition having an antioxidant activity comprising a hydrangea hot water extract as an active ingredient.

More specifically, the present invention is characterized by a cosmetic composition having an antioxidant effect including an active ingredient extracted from the fruit mushroom fruit body,

The hydrangea hydroponic extract is characterized in that it was prepared by extracting the hydrothermal extract for 2 hours and 30 minutes at 95 ℃ using the hydrangea fruit body fruit as a solvent.

On the other hand the present invention described above

Characterized in that the cosmetic composition containing the hydrangea hydrothermal extract in a concentration of 100 to 3000 ppm,

Characterized in that the cosmetic composition having an antioxidant effect comprising the above-mentioned hydroponic extract

The cosmetic composition is characterized in that it is formulated in the form selected from skin lotion, essence cream, lotion, milk lotion.

The cosmetic composition containing the mushroom extract of the present invention not only has excellent DPPH scavenging ability and superoxide anion radical scavenging ability, but also superior linoleic acid peroxidation inhibition rate and high total phenolic content. As well as excellent cell protection, whitening, anti-wrinkle effect can be useful in cosmetic compositions for skin improvement, skin protection and skin aging prevention.

Figure 1 shows the DPPH scavenging ability of the mushroom extract.
Figure 2 shows the superoxide anion radical scavenging ability of the mushroom extract.
Figure 3 shows the linoleic acid oxidation inhibition rate with the time of the mushroom extract.
4 shows a correction curve between absorbances for tannic acid.
Figure 5 shows the phenolic content of the blossom mushroom.

The technical terms and scientific terms used in the present invention can be construed as meaning ordinary meanings understood by those of ordinary skill in the art without departing from the scope of the present invention.

The composition comprising the hydrothermal extract of the blossom mushroom of the present invention has an antioxidant effect.

Blossom mushroom in the present invention uses the fruiting body of the blossom mushroom.

Blossom mushroom extract of the present invention can be prepared by extracting hot water at 95 ℃ for 2 hours and 30 minutes using the mushroom mushroom fruit water as a solvent and concentrated under reduced pressure.

The content of the flower mushroom extract in the composition of the present invention is 100 to 3000 ppm based on the total weight of the composition.

If the content of the mushroom extract in the composition of the present invention is less than 100 ppm the addition effect is insignificant, when added in excess of 3000 ppm there is an uneconomic problem compared to the effect.

Blossom mushroom extract of the present invention shows excellent antioxidant activity. Specifically, DPPH is a water-soluble substance with chemically stable free radicals, absorbs electrons at 517 nm, and when it encounters a substance with antioxidant activity, it gives off electrons and reacts from the original color of purple to yellow. It can also be confirmed that the activity of the matsutake mushroom extract increased as the concentration of the matsutake mushroom extract showed 52% scavenging activity at 1000 ppm of the matsutake mushroom extract of the present invention.

People drink more than 500 g of oxygen a day, of which 4 to 5% has become free radicals, causing stress, disease and aging since the 1950s (http://emcenter.or.kr). Representative materials of active oxygen are unstable in the form of radicals called superoxides, which are more oxidative than oxygen. These superoxides react with free ions in vivo to produce hydrogen peroxide or hydroxyl groups, which are much more damaging to the human body than superoxide. The superoxide has an absorbance at 560 nm, and the resultant treatment of the zinnia mushroom extract of the present invention showed a superoxide scavenging rate of 21.3% at 2000 ppm, and the scavenging rate was increased with increasing concentration.

In addition, linoleic acid is an essential unsaturated fatty acid that keeps the skin moisturized as an emulsifier. It corresponds to vitamin-E (tocopherol) and is also used as a source of soap. Linoleic acid reduces the activity of tyrosinase and melanin in melanosomes. It also prevents creation. Thus, the linoleic acid oxidation inhibition of linoleic acid was treated with 2000 ppm of mushroom extracts to induce automatic oxidation using the reaction mixture, and the oxidation inhibition was observed over time. There was a tendency to decrease drastically, but this is a better result than the tendency to rapidly decrease the vitamin C 1000 ppm treatment after 6 hours.

In addition, the phenolic substance can expect various effects in the body, such as antioxidant effect, antimicrobial effect, anticancer effect by antioxidant action, removal of harmful heavy metals. The phenol content was calculated based on the calibration curve using tannic acid as the standard material. Based on this, the total phenol content of each mushroom concentration was analyzed. As a result, 46.7 mg of total phenol was contained in 1 g of the mushroom.

In addition, the mushroom extract of the present invention can be used as a cosmetic composition with excellent antioxidant efficacy.

Hereinafter, the present invention will be described in more detail based on the following examples. However, the following specific experimental methods and examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1: zinnia mushroom extract

After taking 200 g of fresh fruiting body of the blossom mushroom and drying it, the extract was hydrothermally extracted at 95 ° C. for 2 hours and 30 minutes using 2 L of water as a solvent, and then, the extract was concentrated under reduced pressure.

Test Example 1: Determination of DPPH scavenging activity of the extract

DPPH radical scavenging activity was performed according to the method of Lee et al. (1994). 30 μl of the DPPH reaction solution prepared by dissolving in ethanol at a concentration of 1.5 × 10 ⁻⁴ M and a predetermined concentration was added so that the final volume was 3 ml. After 3 minutes, the absorbance was measured at 517 nm (%). Was calculated.

In other words, the dried blossom mushrooms were extracted with hot water and prepared at a concentration of 125 ppm, 250 ppm, 500 ppm, 750 ppm, and 1000 ppm, reacted with a DPPH reaction solution, and then measured for absorbance at 517 nm to measure DPPH scavenging ability. As shown in Fig. 1, the activity of the mushroom extract was increased as the concentration of the mushroom extract was 52% at 1000 ppm.

Test Example 2: Determination of Superoxide Anion Radical Scavenging Activity of the Flowering Mushroom Extract

Superoxide anion radical scavenging ability It was carried out according to the method of Nishikimi et al. (1972). Add 500 μl of sample, 100 μl of 0.1 M Tris-HCl buffer (pH 8.5), 200 μl of 100 μM PMS, 200 μl of 500 μM NBT, and 400 μl of 500 μM NADH to measure absorbance at 560 nm and for solvent free of sample. The results are shown as percent clearance.

The superoxide has an absorbance at 560 nm, and as a result of treating the mushroom mushroom samples as shown in FIG. 2, the superoxide scavenging rate was 21.3% at 2000 ppm, and the scavenging rate was increased with increasing concentration.

Test Example 3: Measurement of linoleic acid peroxidation inhibition rate of the extract

Linoleic acid peroxidation inhibition rate It was carried out according to the method of Haraguchi et al. (1992). 30 µl of the sample solution at 25 mg / ml concentration, 400 µl of linoleic acid, 800 µl of phosphate buffer, and 770 µl of distilled water were added to form a reaction mixture, which caused automatic oxidation at 40 ° C. 0.1 ml of the reaction solution was taken after 24 hours, and 0.1 ml of ferrous chloride at a concentration of 2.45 mg / ml was added to the mixture mixed with 0.1 ml of 30% NH ₄ SCN and 75% ethanol, and mixed for 3 minutes. The absorbance was measured at to show the antioxidant effect as an inhibition rate (%) for the absorbance of the control group using a solvent.

The linoleic acid oxidation inhibition of linoleic acid was treated with 2000 ppm of the mushroom extract, and the oxidation reaction was induced using the reaction mixture to observe the oxidation inhibition over time, and the results are shown in FIG. 3. As shown in FIG. 3, the increase over time tended to decrease sharply after 27 hours, but this was a better result than the tendency of 1000 ppm vitamin C treatment to rapidly decrease after 6 hours.

Test Example 4: total phenolic content of the extract

Phenol content was measured according to the method of Kim et al. (1993). After mixing 100 µl of the sample solution, 2 ml of 2% Na ₂ CO ₃ and 100 µl of 50% Folin-Ciotalteau reagent, the absorbance was measured at 750 nm after 30 minutes. It calculated based on the calibration curve used.

The phenol content was calculated as shown in FIG. 4 based on the calibration curve using tannic acid as a standard. Based on the results of the analysis of the total phenolic content of the treated mushrooms according to the concentration is shown in FIG. As shown in Figure 5, it can be seen that 46.7 mg of total phenol contained in 1 g of the mushroom.

Preparation Example 1 Preparation of Essence

An essence comprising the mushroom extract of Example 1 was prepared according to the composition of Table 1 below.

[Components of Essence Containing Blossom Mushroom Extract] Ingredient Name Content (wt) A Butylene Glycol 5.00 Sodium hyaluronate 5.00 glycerin 4.00 Abutin 2.00 Example 1 Extract 2.00 Panthenol 2.00 Sodium carbomer 0.30 Methyl paraben 0.2 PEG-60 Hydrogenate Castor Oil 0.1 Adenosine 0.04 B Phenoxyethanol 0.25 Combination fragrance (Bamboo Gardens SG16436) 0.15 C Purified water Balance

Preparation Example 2 Preparation of Cream

Cream containing the mushroom extract of Example 1 was prepared according to the composition of Table 1.

[Components of Cream Containing Blossom Mushroom Extract] Ingredient Name Content (wt) A Abutin 2.00 Butylene Glycol 6.00 Methyl paraben 0.25 glycerin 3.00 Allantoin 0.10 Dipotassium Glycirizate 0.10 Sodium hyaluronate 7.00 Panthenol 0.20 Example 1 Extract 2.00 Sodium carbomer 0.50 Andenosine 0.04 Purslane extract 2.00 B Cetearyl alcohol 1.50 Capric Triglycerides 3.00 Butylene Glycol Dicaprylate 3.00 Jojoba oil 1.00 Macadamia oil 1.00 Petrolatum 1.00 C14-22 alcohol; C12-20 Alkyl Glucoside 3.00 Tocopheryl acetate 0.40 Dimethicone 1.00 Etelparaben 0.20 Propylparaben 0.10 C Phenoxyethanol 0.30 Spices (Bamboo Gardens SG16436) 0.20 D Purified water Balance

Prescription Example 1: Skin elasticity and moisturizing effect of the flower composition containing the mushroom extract

To examine the effect of zinnia mushroom extract on skin elasticity and moisturizing effect, it was applied twice a day to the edge of the left eye of 20 people. The content (ppm) of the flower mushroom extract prepared by Example 1 was varied and the other components were prepared in the same manner as in Preparation Example 1 to prepare an essence. Prepared left eye and untreated right eye edge were used as a control. The skin elasticity of the skin surface was measured by Corneometer CM 80 and Cutometer SEM 474 before and after treatment with the cosmetics containing the mushroom extract 40 days. Skin moisture measurement by Corneometer CM 820 showed the increased moisturizing value in% compared to the control group and the average value of 20 subjects was shown in the results. Skin elasticity measurement by Cutometer SEM 474 is a measure of the depth of wrinkles, the smaller the value is the better elasticity. The elasticity value was shown as a percentage decrease compared to the control, and the average value of 20 subjects was shown in the results. This is shown in Table 3 below.

[Skin Elasticity and Moisturizing Effect of the Mushroom Extract Containing Cosmetic Composition] Blossom Mushroom Extract Content (PPM) Skin moisturizing effect (%) Skin elasticity effect (%) 0 15 20 100 25 35 200 28 40 500 30 42 1000 31 43 1500 32 43 3000 32 44 4000 32 44

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Claims (5)

Blossom Mushroom Extract 2.00wt (%), Butylene Glycol 5.00wt (%), Sodium Hyaluronate 5.00wt (%), Glycerin 4.00wt (%), Abbutin 2.00wt (%), Panthenol 2.00 wt%, sodium carbomer 0.30 wt% methylparaben 0.2 wt% PEG-60 hydrocastate castor oil 0.1 wt% adenosine 0.04 wt% , Essence comprising phenoxyethanol 0.25wt (%), Combination fragrance (Bamboo Gardens SG16436) 0.15wt (%) and remaining amount of purified water Blossom Mushroom Extract 2.00wt (%), Arbutin 2.00wt (%), Butylene Glycol 6.00wt (%), Methylparaben 0.25wt (%), Glycerin 3.00wt (%), Allantoin 0.10 wt (%), dipotassium glycyrizate 0.10 wt (%), sodium hyaluronate 7.00 wt (%), panthenol 0.20 wt (%), sodium carbomer 0.50 wt (%), ande Nosine 0.04 wt%, purslane extract 2.00 wt%, cetearyl alcohol 1.50 wt%, capric triglyceride 3.00 wt%, butylene glycol dicaprylate 3.00 wt% 1.00 wt% jojoba oil, 1.00 wt% macadamia oil, 1.00 wt% petrolatum, 3.00 wt% C14-22 alcohol and C12-20 alkyl glucoside mixture, tocopheryl acetate 0.40 wt (%), Dimethicone 1.00 wt (%), Ethella paraben 0.20 wt (%), Propylparaben 0.10 wt (%), Phenoxyethanol 0.30 wt (%), Perfume (Bamboo Gardens SG16436) Chrysanthemum mushroom extract containing 0.20wt (%) and remaining amount of purified water




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