CN105596229A - Cosmetic with efficacies of resisting ageing, whitening skin and preserving moisture - Google Patents
Cosmetic with efficacies of resisting ageing, whitening skin and preserving moisture Download PDFInfo
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- CN105596229A CN105596229A CN201610083775.9A CN201610083775A CN105596229A CN 105596229 A CN105596229 A CN 105596229A CN 201610083775 A CN201610083775 A CN 201610083775A CN 105596229 A CN105596229 A CN 105596229A
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- 239000002537 cosmetic Substances 0.000 title claims abstract description 50
- 230000002087 whitening effect Effects 0.000 title abstract description 7
- 230000032683 aging Effects 0.000 title abstract description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 27
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 24
- 235000014360 Punica granatum Nutrition 0.000 claims abstract description 23
- 229920001184 polypeptide Polymers 0.000 claims abstract description 22
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 11
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 108010024636 Glutathione Proteins 0.000 claims abstract description 4
- 229960003180 glutathione Drugs 0.000 claims abstract description 4
- 239000004006 olive oil Substances 0.000 claims abstract description 4
- 235000008390 olive oil Nutrition 0.000 claims abstract description 4
- 229940005581 sodium lactate Drugs 0.000 claims abstract description 4
- 239000001540 sodium lactate Substances 0.000 claims abstract description 4
- 235000011088 sodium lactate Nutrition 0.000 claims abstract description 4
- 239000008213 purified water Substances 0.000 claims abstract description 3
- 241000219991 Lythraceae Species 0.000 claims description 24
- -1 D-pantothenyl Chemical group 0.000 claims description 7
- 235000011187 glycerol Nutrition 0.000 claims description 5
- 241000894007 species Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 abstract description 6
- 229930003427 Vitamin E Natural products 0.000 abstract description 3
- 239000011149 active material Substances 0.000 abstract description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 235000019165 vitamin E Nutrition 0.000 abstract description 3
- 229940046009 vitamin E Drugs 0.000 abstract description 3
- 239000011709 vitamin E Substances 0.000 abstract description 3
- 239000011703 D-panthenol Substances 0.000 abstract 1
- SNPLKNRPJHDVJA-ZETCQYMHSA-N D-panthenol Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCCO SNPLKNRPJHDVJA-ZETCQYMHSA-N 0.000 abstract 1
- 235000004866 D-panthenol Nutrition 0.000 abstract 1
- 244000294611 Punica granatum Species 0.000 abstract 1
- 229960003949 dexpanthenol Drugs 0.000 abstract 1
- 229940045920 sodium pyrrolidone carboxylate Drugs 0.000 abstract 1
- HYRLWUFWDYFEES-UHFFFAOYSA-M sodium;2-oxopyrrolidine-1-carboxylate Chemical compound [Na+].[O-]C(=O)N1CCCC1=O HYRLWUFWDYFEES-UHFFFAOYSA-M 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 22
- 238000012360 testing method Methods 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- 239000003963 antioxidant agent Substances 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 230000003078 antioxidant effect Effects 0.000 description 9
- 235000006708 antioxidants Nutrition 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- 230000003712 anti-aging effect Effects 0.000 description 8
- 150000003254 radicals Chemical class 0.000 description 8
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000003020 moisturizing effect Effects 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 230000007760 free radical scavenging Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000001914 filtration Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000012795 verification Methods 0.000 description 3
- YCSMVPSDJIOXGN-UHFFFAOYSA-N CCCCCCCCCCCC[Na] Chemical compound CCCCCCCCCCCC[Na] YCSMVPSDJIOXGN-UHFFFAOYSA-N 0.000 description 2
- 229920001503 Glucan Polymers 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 206010040880 Skin irritation Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- 238000011033 desalting Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000036556 skin irritation Effects 0.000 description 2
- 231100000475 skin irritation Toxicity 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 208000001126 Keratosis Diseases 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 241000233805 Phoenix Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241001083505 Punica Species 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000002605 anti-dotal effect Effects 0.000 description 1
- 230000001153 anti-wrinkle effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000000254 damaging effect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000013100 final test Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 208000007578 phototoxic dermatitis Diseases 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 150000004040 pyrrolidinones Chemical class 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/83—Electrophoresis; Electrodes; Electrolytic phenomena
Abstract
The invention discloses cosmetic with efficacies of resisting ageing, whitening the skin and preserving moisture. The cosmetic is prepared from the following raw materials in parts by weight: 0.1 part of pomegranate polypeptide, 2 parts of olive oil, 0.5 part of D-panthenol, 0.5 part of vitamin E, 0.1 part of glutathione, 1 part of sodium pyrrolidonecarboxylate, 2 parts of glycerinum, 1 part of sodium lactate, 0.1 part of essence and 5 parts of purified water. The cosmetic disclosed by the invention is low in price and abundant in sourceof raw materials, natural, mild and free of thrill to skin; a plurality of active materials synergistically act on the skin; and the effect of the cosmetic disclosed by the invention is superior to that of common cosmetic.
Description
Technical field
The present invention relates to a kind of cosmetics, for anti-aging, whitening, the moisturizing of skin.
Background technology
Skin is to be covered in ー vitals of human body surface, by the appendicle of epidermis, corium, hypodermis and skinOfficial forms. Skin is the natural cover for defense together between external environment and body, and the organ in protection body is avoided temperature and humidityThe impact changing, and the impact of external environment (as ultraviolet ray and pollutant etc.), but the protective effect of skin is limited, when external influence exceedes certain degree, skin aging is inevitable with regard to Cheng Wei ー kind.
Anti-aging cosmetics has accounted for the more than 50% of Cosmetic Market. One of main factor of skin senescence is skinThe skin photosensitiveness that caused by ultra-violet radiation is aging. The light aging of skin is because free-radical contents in skin increases, freelyBase can damage biomembrane, impels elastomer that crosslinked and polymerization occur, and accelerates the aging of skin. Antagonism and elimination free radical preventThe damaging action of free radical, often realizes by antioxidant. Current antioxidant is divided into two classes: the one, and non-enzyme is anti-oxidantAgent, as VE, VC etc.; Another kind of is enzyme antioxidant, and wherein most popular is superoxide dismutase. This two classes antioxygenAfter agent joins in cosmetics, the poor stability of enzyme antioxidant in cosmetics, and because molecular weight is larger, infiltrateSkin is restricted, and therefore its use still has limitation. Therefore, use little molecule non-enzyme antioxidant more good. AndVitamin series non-enzyme antioxidant, due to less stable, has also limited its application in cosmetics. Grinding in recent yearsStudy carefully discovery, the Molecular antioxidant substances extracting from plant, Chinese herbal medicine etc. is such as polypeptide is Wheat Protein a bit, stillIts effect is not also clearly.
Pomegranate is Punicaceae (Punicaceae), Punica, machaka or dungarunga. Pomegranate is nutritious, sugar contentUp to 11% 14%, tartaric acid 0.4% 1.0%, moisture content 79%, protein, fat respectively account for 0.6%, and ascorbic content isApple and pears 23 times, especially outstanding with the content of phosphorus, every hectogram reaches 145 milligrams, in fruit, comes out at the top. Juice of my pomegranateHaematochrome has certain removing ability to DPPH, and its antioxidation and Vc are close, the juice of my pomegranate haematochrome pair of 2.Omg/mlThe clearance rate of DPPH reaches 91.48%, shows that juice of my pomegranate haematochrome has good antioxidation activity. Juice of my pomegranate haematochrome adds toIn cosmetic formulations, find after tested: these cosmetics are after using 28 days, and epidermis smooth cut is dispelled obviously, tight skin system promotes,The effect delaying senility is obvious. But, not yet ripe as basic cosmetics exploitation taking pomegranate at present, there is not relevant utilization.Have great application prospect.
Summary of the invention
The object of this invention is to provide a kind of anti-aging cosmetics taking pomegranate extraction polypeptide as main active, utilizeThe pomegranate polypeptide extracting adds in cosmetic formulations, is equipped with the cosmetics that other auxiliary material and component are prepared from. Through surveyingTry these cosmetics and there is good antidotal effect.
The anti-aging cosmetics of this invention Ti Chu ー kind external application, is characterized in that, is made up of each component of following weight portion:0.1 part of pomegranate polypeptide, 2 parts, olive oil, 0.5 part of D-pantothenyl aleohol, 0.5 part of vitamin E, 0.1 part of glutathione, pyrrolidones carboxylic1 part, acid sodium, 2 parts of glycerine, 1 part of sodium lactate, 0.1 part, essence, 5 parts of purified water.
The present invention provides a kind of preparation method of pomegranate polypeptide in addition, and (1) cleans up pomegranate, rubs, and squeezes the juice, and addsPapain and trypsase, enzyme concentration 20000IU/g pomegranate, 55 ° of C of hydrolysis temperature, pH value 7.0, enzymolysis time 3h, enzymolysis92 ° of C enzyme 13min that goes out after completing;
(2) material filtering going out after enzyme is removed insoluble matter, obtains solution, and gained polypeptide solution adds 4% charcoal absorption decolouring,Carry out polypeptide separation with glucan G-50 (SephadexG-50), 20mmol/LHCl eluant solution, flow velocity 1.3mL/ minute,Collect respectively the eluted product of different time sections, regulator solution is to pH7.0,10000 revs/min centrifugal 15 minutes, set through macroporeAfter fat DA201-C desalting processing, Vacuum Concentration, supernatant freeze drying is for subsequent use; Solidifying through lauryl sodium sulfate-polyacrylamideGel electrophoresis (SDS-PAGE), reclaims the band of little molecular weight, wherein passes through functional verification, and the sequence that obtains altogether 60 little peptides hasAntidotal function. According to the peak separation time different in chromatographic column, can obtain in batches corresponding little peptide, also can manually closeBecome described polypeptide. The sequence of described polypeptide is as shown in SEQIDNO:1-60.
The invention has the beneficial effects as follows:
Cosmetic material of the present invention is cheap, and source is abundant. Pomegranate polypeptide is combined with other active materials, can better growSupport skin, effectively prevent and anti-wrinkle is generated, increase skin elasticity, delay senility. It is raw material that the present invention adopts pomegranate polypeptideThe cosmetics that prepare, natural, gentle, non-stimulated to skin, and also various active material acts synergistically on skin, its effectBe better than ordinary cosmetics.
Detailed description of the preferred embodiments
Following examples have illustrated each aspect of the present invention content. They only, in order to explanation, not delimit the scope of the invention.
The preparation of embodiment 1 polypeptide
(I) pomegranate is cleaned up, rub, squeeze the juice, add papain and trypsase, enzyme concentration 20000IU/g pomegranate,55 ° of C of hydrolysis temperature, pH value 7.0, enzymolysis time 3h, 92 ° of C enzyme 13min that goes out after enzymolysis completes;
(2) material filtering going out after enzyme is removed insoluble matter, obtains solution, and gained polypeptide solution adds 4% charcoal absorption decolouring,Carry out polypeptide separation with glucan G-50 (SephadexG-50), 20mmol/LHCl eluant solution, flow velocity 1.3mL/ minute,Collect respectively the eluted product of different time sections, regulator solution is to pH7.0,10000 revs/min centrifugal 15 minutes, set through macroporeAfter fat DA201-C desalting processing, Vacuum Concentration, supernatant freeze drying is for subsequent use; Solidifying through lauryl sodium sulfate-polyacrylamideGel electrophoresis (SDS-PAGE), reclaims the band of little molecular weight, wherein passes through functional verification, and the sequence that obtains altogether 60 little peptides hasAntidotal function. The sequence of described polypeptide is as shown in SEQIDNO:1-60, and corresponding name is called HFP-1 ~ HFP-respectively60。
The preparation of embodiment 2 cosmetics
Get respectively pomegranate polypeptide HFP-10.1 part, dissolve with 2 parts, 2 parts of glycerine and olive oil. Under agitation add successively0.5 part of D-pantothenyl aleohol, 0.5 part of vitamin E, 0.1 part of glutathione, 1 part of pyrrolidone sodium carboxylate, 1 part of sodium lactate, essence0.1 part, fully stir and make to dissolve completely, mix, the pH to 5.5 of regulator solution 6.5, finally supplies and joins by 5 parts of purified waterSide's total amount, aseptic filtration, packing, is finished product cosmetics.
The preparation of embodiment 3 cosmetics HFP-2 ~ 60
According to the method for embodiment 2, pomegranate polypeptide is replaced with respectively to HFP-2 ~ 60 totally 59 kinds of forms, all the other components and preparation sideMethod is identical with embodiment 2, prepares described cosmetics.
Embodiment 4 contrasts the preparation of cosmetics
According to the method for embodiment 2, wherein do not add corresponding pomegranate polypeptide, all the other components and method are identical with embodiment 2, systemFor obtaining corresponding cosmetics.
Embodiment 5 security verifications
Test material and method
Animal: regular grade animal used as test White Rabbit. Body is looked into animal no abnormality seen.
Environmental condition: room temperature: 22 ° of C-23 ° C, relative humidity: 66%-68%;
Test method:
According to the test of " cosmetics health specification " (version in 2007) acute skin irritation, test first 24 hours, by experimental animalBack backbone diamond wool is cut, 3 centimetres of X3 centimetre of unhairing scope. Get and testedly directly spread upon right side skin without 0.5 gram of originalityUpper, cover with clean gauze, with nonirritant immobilization with adhesive tape 2 hours. The negative contrast of an other side. Remove 1 after tested materialHour, within 24 hours, 48 hours 72 hours, observe animal subject contact site dermoreaction keeping a record.
Conclusion:
Under this experimental condition, the cosmetics that tested material contains pomegranate polypeptide sting by skin White Rabbit acute skin irritation testSharp strength grading is nonirritant. Also nonirritant of contrast cosmetics.
Embodiment 6 cosmetics moisturizing degree and moisture preserving time detect
MMV (moisturemeasurementvalue) method detects the ability of MMV value reflection keratoderma in conjunction with water. AdoptThe aquation of CM825 type skin oil-water soda acid tester test skin. Its measuring principle is because cuticula contains in a large numberElectrolyte, exist the electrolyte in water to there is electric conductivity, this phenomenon is also created in skin surface, by test probe and skinAfter skin contact, the variation of electrical conductivity reflects the hydration status of skin.
Experimenter is healthy without dermopathic volunteer. Experimental condition is that the temperature and humidity of test period environment is constant,Temperature is 25 ° of C ± 2 ° C, and relative humidity is 40% ~ 60%.
Choose health without 62 of dermopathic volunteers, test. Method of testing is: 1. determine 4 surveys of left and right forearmExamination region, region area is 4cmX4cm, tests respectively the blank value in this region. 2. sample is coated on to test zone, consumption is2mg/cm2. 3. after using, 120min carries out final test, and 5 points of every domain test, average. Moistening effect is evaluatedJudgement schematics: in relatively moisturizing rate/%=(P sample t=x-P blank)/(P mark t=x-P blank) X100% formula: P sample t=x-usesThe electrical conductivity of product after certain hour; P blank-the be not coated with electrical conductivity of sample; Object of reference after P mark t=x-use certain hourElectrical conductivity, moisturizing rate situation (%) result of cosmetics of the present invention and glycerine comparison is as shown in the table:
From above form, the moisturizing rate of cosmetics of the present invention, apparently higher than the moisturizing situation of glycerine, also illustrates this simultaneouslyBright polypeptide has significant facilitation for the moistening effect that improves cosmetics.
Embodiment 6 senile-resistant efficacy evaluation tests
The anti-ageing effect detection of cosmetics to skin
Free radical is the intermediate product of biochemical reaction in vital movement process, with the aging relevant Biological Free Radicals master of lifeIf some oxygen containing free radicals, antioxidant can provide proton to peroxide radical, thereby can suppress lipid from oxygenThe chain reaction of changing. Conventionally make DPPH check antioxidant to put forward protogenic ability. DPPH is a kind of very stable freedomBase, its methanol solution has extremely strong absorbance at 517nm place, but when oxidized dose (AH) or free radical (RO when reduction, its suctionLuminosity can reduce or disappear. Thus, can utilize sample to judge in the absorbance at 517nm place whether sample has the free radical of removingAbility.
Get cosmetics, be configured to the solution of 100mg cosmetics/ml with PBS, respectively with 3.8ml methanol solution, Iml(DPPH) radical methanol solution (2Xl0-4mol/L), is fully mixed evenly, and leaves standstill after 30min, with spectrophotometric instrumentation517nm place absorbance, and calculate its free radical scavenging activity with formula. Free radical scavenging activity (%)=[(Ac-As/Ac)] X100%As: the sample that contains determinand is in the absorbance at 517nm place; Ac: not containing the sample of determinand in the absorbance at 517nm place notThe free radical scavenging activity (%) of the Chinese medical extract of same volume, its result following (% of unit of result):
As can be seen from the table, cosmetics of the present invention are higher for free radical scavenging activity. Illustrate that cosmetics of the present invention haveGood anti-ageing ability.
Embodiment 7 face cream whitening effects detect
Select each 61 of the darker men and women volunteer of the colour of skin, sooner or later after clean to right face cosmetics of the present invention, carry out the morning and eveningInterval 12h smears, and left face in contrast, is only smeared the cosmetics of embodiment 4, and surrounding on probation, fills in examination according to sensory evaluationUse evaluation table. It is remarkable that best result 4 is divided into effect, is minimumly divided into 1, and contrast effect is not obvious. Enter in 4th week utilization card side respectivelyRow statistics.
Find by detection, the cosmetics that contain HFP-1 ~ 60 polypeptide are using after surrounding, and right face has occurred significantlySpot desalination effect (P < 0.05). Above data declaration the present invention has effect of whitening. And right face uses the left and right face of glycerinePortion does not have significant difference, illustrates that cosmetics of the present invention have comparatively significant white-skinned face function.
Sequence table
< 110 > turn round and look at silver-colored phoenix
That mono-kind of < 120 > has is anti-aging, the cosmetics of whitening, moisture-keeping efficacy
〈160〉60
〈210〉1
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-1
RFIVHSLPKPSMHPAQRRR
〈210〉2
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-2
PQQCVLVQTTWMSLEGGRS
〈210〉3
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-3
PPRPYYPQQTEFSHDQRK
〈210〉4
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-4
RLLSSDWNQLQEYWRYMV
〈210〉5
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-5
WRHRNWQGQGLGRGDDD
〈210〉6
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-6
DRDMLIAQTMKGTVTS
〈210〉7
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-7
GIFFTETLNSNCTETYP
〈210〉8
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-8
LWAKWWHLIYPHHYIH
〈210〉9
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-9
MKHWLRNEWRIERVMDQQA
〈210〉10
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-10
PDLMYSYPPTIQIIGLNT
〈210〉11
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-11
AMGKMTNFVLSHNPTST
〈210〉12
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-12
TITYSTQLIRRYRSHM
〈210〉13
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-13
DSCVQAIHWMWWDEVLSGY
〈210〉14
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-14
INKPWQQSGHFADSWKRG
〈210〉15
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-15
VMFTDIKCPCQFAHMND
〈210〉16
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-16
LPIPRKTSPWCIWSYN
〈210〉17
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-17
RVSMYWERSDNGFHQRQES
〈210〉18
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-18
LPGDTHAPWWWRPVLIAK
〈210〉19
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-19
AMHGMFSTEGTPQEDAP
〈210〉20
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-20
RSHWHEAWHLVCIGGH
〈210〉21
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-21
IPHQELWQKYSQIFLPWTS
〈210〉22
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-22
RLESKRIPFQRGEPRGPMY
〈210〉23
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-23
EWCHQCWMCGVAQMWGHF
〈210〉24
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-24
PYMAGPPRDREGIHFLH
〈210〉25
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-25
EYEGGWPNEQAPAPKG
〈210〉26
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-26
NPPHRCADHQQEVMVSFH
〈210〉27
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-27
KKFFTEWGVGSQQGAPN
〈210〉28
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-28
LFIHCRVKKRMTCHEW
〈210〉29
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-29
EMIWKGRRTPVTDIARPLV
〈210〉30
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-30
NPWRCRVMRPERCHDINQ
〈210〉31
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-31
IWHMCTKTWRFIDSFEK
〈210〉32
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-32
WVSRWVMFSPHPLPVM
〈210〉33
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-33
MGIRSWWCILMIWQHPRQY
〈210〉34
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-34
IITQYVYPDLWYVQPWGI
〈210〉35
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-35
LSDFTWMNLREGWGGVE
〈210〉36
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-36
VYSHRYCLTWEHNLHA
〈210〉37
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-37
GYHSHGLWHFRWHLIYMR
〈210〉38
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-38
VFRNTWWVEHYDGTSNR
〈210〉39
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-39
LQLPRVPTHMYPSHLN
〈210〉40
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-40
VYDLWTDQQSWDFGRHIPP
〈210〉41
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-41
QYRNPEFQHNSWTSDIKNI
〈210〉42
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-42
WDHARRWHHDYEWPRSCRR
〈210〉43
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-43
KMEMHDDYSYKFICSQQWG
〈210〉44
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-44
GSWLNNPRNSVGDPECFG
〈210〉45
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-45
FKGGHPDQVDFKRLRYQHP
〈210〉46
〈211〉19
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-46
PHNMMLVHLVLHSGNQYGH
〈210〉47
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-47
FCSWQFPDELCYTSISSY
〈210〉48
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-48
IGETRCQQNQFKLLRGG
〈210〉49
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-49
PKPGANIIYERECPPY
〈210〉50
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-50
DQWSWPQEHKLPSRGSQQ
〈210〉51
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-51
NCEGTKRISELICIIKH
〈210〉52
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-52
NTCWSRDHMICFWHVC
〈210〉53
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-53
SSINQHQYGNRAESHLM
〈210〉54
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-54
IWWVLFFPQIYWTGPH
〈210〉55
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-55
EYKSSLHLGRWSWWSMNG
〈210〉56
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-56
TDPFNFNSMTYHRRHCV
〈210〉57
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-57
CHTYPSPLNAYHWFKS
〈210〉58
〈211〉18
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-58
DKQGQILTETKKSSQEQC
〈210〉59
〈211〉17
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-59
WGSVTSRMGHVADPQLH
〈210〉60
〈211〉16
〈212〉PRT
< 213 > artificial sequences
〈400〉HFP-60
NHCMHFHNCYHPCRCW
Claims (4)
1. cosmetics, is characterized in that, comprise each component of following weight portion: 2 parts, olive oil, 0.5 part of D-pantothenyl aleohol, dimensionRaw plain E0.5 part, 0.1 part of glutathione, 1 part of pyrrolidone sodium carboxylate, 2 parts of glycerine, 1 part of sodium lactate, 0.1 part, essence,5 parts of purified water.
2. cosmetics as claimed in claim 1, is characterized in that: also comprise 0.1 part of pomegranate polypeptide.
3. cosmetics as claimed in claim 2, is characterized in that, described pomegranate peptide sequence is respectively as SEQIDNO:1-60 arbitrary shown in.
4. a peptide species, its sequence is as shown in SEQIDNO:1-60 is arbitrary.
Priority Applications (17)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610083775.9A CN105596229B (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226372.XA CN106806163A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226360.7A CN106726676A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226358.XA CN106726675A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226367.9A CN106821804A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226369.8A CN106726678A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226371.5A CN106726679A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226368.3A CN106880527A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226366.4A CN106880526A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226872.3A CN106880528A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226376.8A CN106726680A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226377.2A CN106821787A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226365.XA CN106726677A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226362.6A CN106806162A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201810543087.5A CN108578277A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226363.0A CN106821786A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226364.5A CN106880525A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
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| CN201610083775.9A CN105596229B (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
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| CN201710226377.2A Division CN106821787A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226362.6A Division CN106806162A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226376.8A Division CN106726680A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226372.XA Division CN106806163A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226365.XA Division CN106726677A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226371.5A Division CN106726679A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226367.9A Division CN106821804A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226360.7A Division CN106726676A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226872.3A Division CN106880528A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201810543087.5A Division CN108578277A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226369.8A Division CN106726678A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226368.3A Division CN106880527A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226363.0A Division CN106821786A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226366.4A Division CN106880526A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226364.5A Division CN106880525A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226358.XA Division CN106726675A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
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| Publication Number | Publication Date |
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| CN105596229A true CN105596229A (en) | 2016-05-25 |
| CN105596229B CN105596229B (en) | 2017-05-31 |
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| CN201710226363.0A Withdrawn CN106821786A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226365.XA Withdrawn CN106726677A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226368.3A Pending CN106880527A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226369.8A Pending CN106726678A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226372.XA Pending CN106806163A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226364.5A Withdrawn CN106880525A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226358.XA Pending CN106726675A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201810543087.5A Pending CN108578277A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201610083775.9A Active CN105596229B (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226376.8A Withdrawn CN106726680A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226872.3A Withdrawn CN106880528A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226371.5A Pending CN106726679A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226362.6A Pending CN106806162A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226367.9A Pending CN106821804A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226366.4A Pending CN106880526A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226360.7A Pending CN106726676A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226377.2A Withdrawn CN106821787A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
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| CN201710226365.XA Withdrawn CN106726677A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226368.3A Pending CN106880527A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226369.8A Pending CN106726678A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226372.XA Pending CN106806163A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226364.5A Withdrawn CN106880525A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226358.XA Pending CN106726675A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201810543087.5A Pending CN108578277A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
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| CN201710226872.3A Withdrawn CN106880528A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226371.5A Pending CN106726679A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226362.6A Pending CN106806162A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226367.9A Pending CN106821804A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226366.4A Pending CN106880526A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226360.7A Pending CN106726676A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
| CN201710226377.2A Withdrawn CN106821787A (en) | 2016-02-13 | 2016-02-13 | A kind of cosmetics with anti-aging, whitening, moisture-keeping efficacy |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106137785A (en) * | 2016-08-03 | 2016-11-23 | 夏献民 | A kind of skin care item promoting skin repair to regenerate |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112294720A (en) * | 2020-11-12 | 2021-02-02 | 珂蓝(重庆)化妆品有限公司 | Composition for protecting skin from pigment and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101677913A (en) * | 2007-05-10 | 2010-03-24 | 诺伊堡皮肤护理两合公司 | Surfactant-free foam formulations |
| CN103251524A (en) * | 2013-05-06 | 2013-08-21 | 广州立白企业集团有限公司 | Foam facial mask composition and preparation method thereof |
| CN103860453A (en) * | 2014-04-11 | 2014-06-18 | 六安市丹皇生物科技有限责任公司 | Energizing and firming cream and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2607142C (en) * | 2005-05-05 | 2014-03-25 | Genencor International, Inc. | Personal care compositions and methods for their use |
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2016
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- 2016-02-13 CN CN201710226365.XA patent/CN106726677A/en not_active Withdrawn
- 2016-02-13 CN CN201710226368.3A patent/CN106880527A/en active Pending
- 2016-02-13 CN CN201710226369.8A patent/CN106726678A/en active Pending
- 2016-02-13 CN CN201710226372.XA patent/CN106806163A/en active Pending
- 2016-02-13 CN CN201710226364.5A patent/CN106880525A/en not_active Withdrawn
- 2016-02-13 CN CN201710226358.XA patent/CN106726675A/en active Pending
- 2016-02-13 CN CN201810543087.5A patent/CN108578277A/en active Pending
- 2016-02-13 CN CN201610083775.9A patent/CN105596229B/en active Active
- 2016-02-13 CN CN201710226376.8A patent/CN106726680A/en not_active Withdrawn
- 2016-02-13 CN CN201710226872.3A patent/CN106880528A/en not_active Withdrawn
- 2016-02-13 CN CN201710226371.5A patent/CN106726679A/en active Pending
- 2016-02-13 CN CN201710226362.6A patent/CN106806162A/en active Pending
- 2016-02-13 CN CN201710226367.9A patent/CN106821804A/en active Pending
- 2016-02-13 CN CN201710226366.4A patent/CN106880526A/en active Pending
- 2016-02-13 CN CN201710226360.7A patent/CN106726676A/en active Pending
- 2016-02-13 CN CN201710226377.2A patent/CN106821787A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101677913A (en) * | 2007-05-10 | 2010-03-24 | 诺伊堡皮肤护理两合公司 | Surfactant-free foam formulations |
| CN103251524A (en) * | 2013-05-06 | 2013-08-21 | 广州立白企业集团有限公司 | Foam facial mask composition and preparation method thereof |
| CN103860453A (en) * | 2014-04-11 | 2014-06-18 | 六安市丹皇生物科技有限责任公司 | Energizing and firming cream and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106137785A (en) * | 2016-08-03 | 2016-11-23 | 夏献民 | A kind of skin care item promoting skin repair to regenerate |
| CN106137785B (en) * | 2016-08-03 | 2019-05-17 | 夏献民 | A kind of regenerated skin care item of promotion skin repair |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106880525A (en) | 2017-06-23 |
| CN106821786A (en) | 2017-06-13 |
| CN106726679A (en) | 2017-05-31 |
| CN105596229B (en) | 2017-05-31 |
| CN106726675A (en) | 2017-05-31 |
| CN106726678A (en) | 2017-05-31 |
| CN106726677A (en) | 2017-05-31 |
| CN106726680A (en) | 2017-05-31 |
| CN106806162A (en) | 2017-06-09 |
| CN106726676A (en) | 2017-05-31 |
| CN106821787A (en) | 2017-06-13 |
| CN106821804A (en) | 2017-06-13 |
| CN106880526A (en) | 2017-06-23 |
| CN108578277A (en) | 2018-09-28 |
| CN106880527A (en) | 2017-06-23 |
| CN106806163A (en) | 2017-06-09 |
| CN106880528A (en) | 2017-06-23 |
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