CN106688845A - Dendrobium huoshanense seedling domestication method - Google Patents

Dendrobium huoshanense seedling domestication method Download PDF

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Publication number
CN106688845A
CN106688845A CN201611046586.0A CN201611046586A CN106688845A CN 106688845 A CN106688845 A CN 106688845A CN 201611046586 A CN201611046586 A CN 201611046586A CN 106688845 A CN106688845 A CN 106688845A
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Prior art keywords
seedling
sponge
dendrobidium huoshanness
seedlings
water
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CN201611046586.0A
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戴亚峰
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a dendrobium huoshanense seedling domestication method. The dendrobium huoshanense seedling domestication method comprises the following steps of greenhouse construction, seedling bed frame construction, planting medium preparation, planting matrix preparation, planting medium and planting matrix pretreatment, seedling planting, seedling growth and seedling pulling aftertreatment. The dendrobium huoshanense seedling domestication method has the advantages that the water content is high, the water holding capacity is good, not only can water needed by normal growth of dendrobium huoshanense be provided, but also it can be guaranteed that roots of the dendrobium huoshanense cannot be rotten due to too much water, and therefore the dendrobium huoshanense seedling domestication cycle is greatly shortened; the dendrobium huoshanense seedling domestication cycle is shortened by a half, and therefore seedlings are short in buffer time and high in growth speed after being cultivated; in addition, usage of a cultivation matrix is reduced in the cultivation process, recycling of the medium is achieved in the cultivation process, and therefore both the cost and destruction of the ecological environment are reduced; furthermore, if the seedlings are transplanted after being directly domesticated through the method, the survival rate can reach up to 100%.

Description

A kind of method of Dendrobidium huoshanness seedling domestication
Technical field
The present invention relates to the growing and cultivation technical field of the stem of noble dendrobium, more particularly to a kind of method that Dendrobidium huoshanness seedling is tamed.
Background technology
Dendrobium orchid family herbaceos perennial.Dendrobidium huoshanness is commonly called as " rice dry measure used in former times ", is mainly distributed on the Anhui west and south suddenly Mountain, because the compositions such as the alkaloid contained by it, Thick many candies, amino acid, trace element have strong kidney strengthening the essence, thick stomach, suppression tumour The effects such as cell, hypoglycemic, strengthen immunity, alleviation physical fatigue, it is always treated as first of nine big " celestial grass ".
For a long time, because Dendrobidium huoshanness growth conditions harshness and the excessive harvesting of people, the species are endangered. Numerous companies and scientific research institutions by modern biotechnology tissue culture technology and modern biotechnology, realize to the conservation of the stem of noble dendrobium and Rational exploitation and utilization, its general process is:Tissue culture, hardening, domestication, cultivation, harvesting, deep processing.So far, stem of noble dendrobium kind The sale of seedling mainly has tissue-cultured seedling and two kinds of seedling of domestication, and tissue-cultured seedling is the seedling obtained by tissue culture, and seedling cultivation management difficulty is big, Survival rate is low;Domestication seedling is then seedling of the tissue-cultured seedling after cultivation is survived, and transplanting survival rate is high, and management is simple, cultivates risk It is small;The matrix of wherein existing technology seedling domestication is mainly based on bark.
Existing Dendrobidium huoshanness seedling domestication facility is, it is necessary to bark matrix and cultivation as the facility of Dendrobidium huoshanness cultivation Training facility, wherein bark matrix is often tamed a seedling and will be emptied once, it is impossible to is reused, high cost, and is caused Into bark shortage of resources, waste serious;Additionally, with bark as Dendrobidium huoshanness seedling domestication matrix, acclimation period is more long:Tree The bad control of water content of skin, water-holding capacity is poor, and seedling plants rear dry tack free, can just take root within general 15 days, one month or so Could germinate, could at least be grown completely to 3 months or so roots and bud in the case where humiture is suitable;If frequency of watering mistake Many, air humidity is excessive, can also cause the rotten phenomenon of rotten leaf.
The content of the invention
The technical problems to be solved by the invention are that a kind of water content of offer is high, and water-holding capacity is good, can not only provide The moisture of the normal growth of the stem of noble dendrobium, moreover it is possible to ensure that it will not be rotten because of excess moisture such that it is able to greatly shorten Dendrobidium huoshanness The method of the Dendrobidium huoshanness seedling domestication of the acclimation period of seedling.
In order to solve the above technical problems, the present invention provides a kind of method of Dendrobidium huoshanness seedling domestication, comprise the following steps:
(1) greenhouse builds
Greenhouse is built, it is the sunshade net of 60%-80% that plastic foil is covered on ceiling with shading rate;
(2) structure of seedling raising bedstead
Fixed seedling bed is made with plastic deeping, then it is maked somebody a mere figurehead in ground using hollow concrete block;
(3) preparation of medium is planted
Sponge is cut to thickness for 10-20cm, then at sponge by selection sponge as the medium of Dendrobidium huoshanness domesticating and cultivating One side be provided with least one opening, then carry out soaking disinfection with low toxicity bactericide and be provided with the one of opening face up it is flat It is put on the seedling bed made;
(4) preparation of planting matrix
Selection pasture and water are impregnated with completely as planting matrix with low toxicity bactericide, are dried standby;
(5) pretreatment of plantation medium and matrix
Before seedling plantation, the planting matrix pasture and water that will first dry are completely soaked wetting with the nutrient solution for having configured, then use Water spray fills and sponge drenches;
(6) seedling plantation
By Dendrobidium huoshanness seedling with the one clump of packet of 4-6 roots, the root of seedling will be snarled with the soaked pasture and water of nutrient solution, filled in In the opening that sponge above to seedling bed is reserved, fixed compacting;After plantation, adjustment control greenhouse temperature of shed, seedbed humidity, Air humidity and intensity of illumination simultaneously enter water-filling, fertilizer, plant disease management to the seedling after plantation;
(7) seedling early growth and the post processing that lifts seedlings
Kind of a seedling rooting, germination and crescent moon growing state are observed and recorded, seedling domestication work is completed;Treat that seedling domestication is completed Afterwards, it is lifted seedlings and the post processing that lifted seedlings.
Preferably, the greenhouse is high 3-5 meters, 1-1.5 meters of seedling bed bedside;The seedling bed bed is long less than or equal to big Canopy canopy is long.
Preferably, plastic deeping is welded into fixed seedling bed by angle steel in the step (2).
Preferably, the Opening length of sponge, width, depth are 2cm in the step (3).
Preferably, the opening of sponge is equally spaced in the step (3), spacing 2cm.
Preferably, low toxicity bactericide is 750 times of thiophanate methyl in the step (3) and step (4).
Preferably, greenhouse temperature of shed is adjusted to 18 DEG C -30 DEG C in the step (6), seedbed humidity be adjusted to 45 ﹪ - 55 ﹪, air humidity is adjusted to 50 ﹪ -60 ﹪, and intensity of illumination is adjusted to 10000-20000LUX.
Preferably, step (6) reclaimed water, fertilizer, plant disease management mode are respectively:After the stem of noble dendrobium is planted, seedling bed sponge Below 2cm keeps moistening, air humidity to remain 50 ﹪ -60 ﹪;One time of nutrition liquid is sprayed weekly;During daily disease, every ten days Spray the carbendazim or 750 times of thiophanate methyl of 500 times.
Preferably, nutrient solution is the 1/2MS nutrient solutions for being not added with sucrose and hormone in the step (5) and step (6).
Preferably, the mode of lifting seedlings is in the step (7):In the opening that pasture and water and seedling are reserved from sponge on seedling bed Extract together.
Preferably, lifted seedlings in the step (7) post processing include lift seedlings after seedling process with lift seedlings after facility process;It is described Seedling process mode is after lifting seedlings:The seedling that will be extracted is sold or is transplanted as domestication commercial seedling;It is described lift seedlings after at facility Reason mode is:Sponge on seedling bed is cleaned, treatment is dried and 2-3 is disinfected under the ozone environment of high concentration Hour, reuse.
Preferably, the time of the seedling plantation is spring or autumn.
The advantage of the invention is that:Sponge is selected as the medium of Dendrobidium huoshanness domesticating and cultivating, there is provided a kind of water content Height, water-holding capacity is good, can not only provide the moisture of the normal growth of the stem of noble dendrobium, moreover it is possible to ensure that it will not be rotten because of excess moisture Root such that it is able to which the method for greatly shortening the Dendrobidium huoshanness seedling domestication of the acclimation period of Dendrobidium huoshanness seedling, this method is direct The acclimation period of Dendrobidium huoshanness seedling is totally shortened into half, buffer time is short after cultivating seedling, fast growth, and Can realize that the saving of cultivation matrix resource in cultivation uses and cultivate the recycling of medium, can not only so reduce into This, also reduces the destruction to ecological environment;Additionally, being transplanted again by the seedling that this method is directly tamed, survival rate is high Up to 100%.
Brief description of the drawings
Fig. 1 is a kind of flow chart of the method for Dendrobidium huoshanness seedling domestication that the embodiment of the present invention 1 is provided.
Specific embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out under premised on technical solution of the present invention Implement, give detailed implementation method and specific operating process, but protection scope of the present invention is not limited to following implementations Example.
Embodiment 1
A kind of method of Dendrobidium huoshanness seedling domestication, as shown in figure 1, comprising the following steps:
(1) greenhouse builds
Greenhouse is built, canopy is high 3 meters, and canopy is wide 4.5 meters, and canopy is long 10 meters, it is 60%- that plastic foil is covered on ceiling with shading rate 80% sunshade net, for rainwater-proof and shuts out the light;
(2) structure of seedling raising bedstead
Welded with angle steel plus plastic deeping make fixed seedling bed, then it is maked somebody a mere figurehead in ground using hollow concrete block, Beneficial to bottom ventilation, wherein 1.5 meters of bedside, bed is long 10 meters;
(3) preparation of medium is planted
Sponge is cut to thickness for 10-20cm, then at sponge by selection sponge as the medium of Dendrobidium huoshanness domesticating and cultivating One side be provided with the opening that six long × wide × depth are respectively 2cm × 2cm × 2cm, spacing is 2cm, neat distribution, is then used 750 times of thiophanate methyl carries out soaking disinfection and is provided with the one of opening and faces up to lie against on the seedling bed made;
(4) preparation of planting matrix
Selection pasture and water are impregnated with completely as planting matrix with 750 times of thiophanate methyl, are dried standby;
(5) pretreatment of plantation medium and matrix
Before seedling plantation, the planting matrix pasture and water that will first dry (are not added with sucrose with hormone with the nutrient solution for having configured 1/2MS nutrient solutions) wetting is completely soaked, then sponge is drenched with water spray filling;
(6) seedling plantation
Dendrobidium huoshanness seedling is grouped with 6 one clump, will be soaked with nutrient solution (being not added with the 1/2MS nutrient solutions of sucrose and hormone) The pasture and water for soaking snarl the root of seedling, in plug to the reserved opening of the sponge above seedling bed, fixed compacting;
It is the most optimal with 28 DEG C by the adjustment control of greenhouse temperature of shed between 18 DEG C -30 DEG C after plantation;Seedbed humidity In 50 ﹪, air humidity maintains 60 ﹪ for control;Illumination direct projection is avoided, sunshade, intensity of illumination adjustment are covered with shade net ceiling It is 15000LUX;
Additionally, the seedling after plantation will carry out in good time water, fertilizer, plant disease management, way to manage is respectively:The stem of noble dendrobium is planted Afterwards, below seedling bed sponge 2cm keeps moistening, air humidity to remain 60 ﹪;Spray weekly one time of nutrition liquid (be not added with sucrose with The 1/2MS nutrient solutions of hormone);During daily disease, the carbendazim or 750 times of thiophanate methyl of 500 times is sprayed within every ten days;
(7) seedling early growth and the post processing that lifts seedlings
Seedling early growth situation is observed and records, wherein the present embodiment seedling early growth situation is:Take root within 3 days, germinate within one week, 2 Individual crescent moon grows up to, so as to complete seedling domestication work;
Treat after the completion of seedling domestication, lifted seedlings and lifted seedlings post processing to it, wherein the mode of lifting seedlings is:By pasture and water and seedling Extracted together in the opening reserved from sponge on seedling bed;The post processing that lifts seedlings include lifting seedlings after seedling process with lift seedlings after at facility Reason;Seedling process mode is after lifting seedlings:The seedling that will be extracted is sold or is transplanted as domestication commercial seedling;Facility treatment after lifting seedlings Mode is:Sponge on seedling bed is cleaned, dry treatment and disinfected under the ozone environment of high concentration 2 it is small When, reuse.
Preferably, the time of above-mentioned seedling plantation is spring or autumn.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.

Claims (10)

1. a kind of method that Dendrobidium huoshanness seedling is tamed, it is characterised in that comprise the following steps:
(1) greenhouse builds
Greenhouse is built, it is the sunshade net of 60%-80% that plastic foil is covered on ceiling with shading rate;
(2) structure of seedling raising bedstead
Fixed seedling bed is made with plastic deeping, then it is maked somebody a mere figurehead in ground using hollow concrete block;
(3) preparation of medium is planted
Sponge is cut to thickness for 10-20cm, then at the one of sponge by selection sponge as the medium of Dendrobidium huoshanness domesticating and cultivating Face is provided with least one opening, then carries out soaking disinfection with low toxicity bactericide and be provided with the one of opening and face up to lie against On the seedling bed made;
(4) preparation of planting matrix
Selection pasture and water are impregnated with completely as planting matrix with low toxicity bactericide, are dried standby;
(5) pretreatment of plantation medium and matrix
Before seedling plantation, the planting matrix pasture and water that will first the dry nutrient solution for having configured is completely soaked wetting, then with spraying water Filling drenches sponge;
(6) seedling plantation
By Dendrobidium huoshanness seedling with the packet of 4-6 roots one clump, the root of seedling will be snarled with the soaked pasture and water of nutrient solution, plug is to educating In the opening that sponge above seedbed is reserved, fixed compacting;After plantation, adjustment control greenhouse temperature of shed, seedbed humidity, air Humidity and intensity of illumination simultaneously enter water-filling, fertilizer, plant disease management to the seedling after plantation;
(7) seedling early growth and the post processing that lifts seedlings
Kind of a seedling rooting, germination and crescent moon growing state are observed and recorded, seedling domestication work is completed;After the completion for the treatment of that seedling is tamed, Lifted seedlings and lifted seedlings post processing to it.
2. the method that a kind of Dendrobidium huoshanness seedling according to claim 1 is tamed, it is characterised in that the greenhouse 3- high 5 meters, 1-1.5 meters of seedling bed bedside;The seedling bed bed is long long less than or equal to greenhouse.
3. the method that a kind of Dendrobidium huoshanness seedling according to claim 1 is tamed, it is characterised in that in the step (3) The Opening length of sponge, width, depth are 2cm.
4. the method that a kind of Dendrobidium huoshanness seedling according to claim 1 is tamed, it is characterised in that in the step (3) The opening of sponge is equally spaced, spacing 2cm.
5. the method for a kind of Dendrobidium huoshanness seedling domestication according to claim 1, it is characterised in that the step (3) and Low toxicity bactericide is 750 times of thiophanate methyl in step (4).
6. the method that a kind of Dendrobidium huoshanness seedling according to claim 1 is tamed, it is characterised in that in the step (6) Greenhouse temperature of shed is adjusted to 18 DEG C -30 DEG C, and seedbed humidity is adjusted to 45 ﹪ -55 ﹪, and air humidity is adjusted to 50 ﹪ -60 ﹪, Intensity of illumination is adjusted to 10000-20000LUX.
7. the method that a kind of Dendrobidium huoshanness seedling according to claim 1 is tamed, it is characterised in that in the step (6) Water, fertilizer, plant disease management mode are respectively:After the stem of noble dendrobium is planted, below seedling bed sponge 2cm keeps moistening, air humidity to remain 50 ﹪ -60 ﹪;One time of nutrition liquid is sprayed weekly;During daily disease, spray within every ten days one time 500 times carbendazim or 750 times Thiophanate methyl.
8. the method for a kind of Dendrobidium huoshanness seedling domestication according to claim 7, it is characterised in that the step (5) and Nutrient solution is the 1/2MS nutrient solutions for being not added with sucrose and hormone in step (6).
9. the method that a kind of Dendrobidium huoshanness seedling according to claim 1 is tamed, it is characterised in that in the step (7) The mode of lifting seedlings is:Extracted together with the opening that pasture and water are reserved with seedling from sponge on seedling bed.
10. the method that a kind of Dendrobidium huoshanness seedling according to claim 9 is tamed, it is characterised in that in the step (7) Lift seedlings post processing include lift seedlings after seedling process with lift seedlings after facility process;It is described lift seedlings after seedling process mode be:To extract Seedling as domestication commercial seedling sell or transplant;It is described lift seedlings after facility processing mode be:Sponge on seedling bed is entered Row cleans, dries treatment and disinfected 2-3 hour under the ozone environment of high concentration, reuses.
CN201611046586.0A 2016-11-23 2016-11-23 Dendrobium huoshanense seedling domestication method Pending CN106688845A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103004445A (en) * 2013-01-06 2013-04-03 戴亚峰 Method for directly seedling dendrobe seedlings by using plastic greenhouse
CN103098691A (en) * 2013-01-18 2013-05-15 六安同济生生物科技有限公司 Dendrobium huoshanense pollution-free wild-imitating planting method
CN203884354U (en) * 2014-05-13 2014-10-22 龚福明 Domestication planting bed special for tissue-cultured seedlings of dendrobium officinale
CN104620836A (en) * 2015-03-03 2015-05-20 云南久丽康源石斛开发有限公司 Dendrobium officinale wild-imitating cultivation method
CN105850690A (en) * 2016-06-27 2016-08-17 广西农业职业技术学院 Dendrobium officinale culture medium

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103004445A (en) * 2013-01-06 2013-04-03 戴亚峰 Method for directly seedling dendrobe seedlings by using plastic greenhouse
CN103098691A (en) * 2013-01-18 2013-05-15 六安同济生生物科技有限公司 Dendrobium huoshanense pollution-free wild-imitating planting method
CN203884354U (en) * 2014-05-13 2014-10-22 龚福明 Domestication planting bed special for tissue-cultured seedlings of dendrobium officinale
CN104620836A (en) * 2015-03-03 2015-05-20 云南久丽康源石斛开发有限公司 Dendrobium officinale wild-imitating cultivation method
CN105850690A (en) * 2016-06-27 2016-08-17 广西农业职业技术学院 Dendrobium officinale culture medium

Non-Patent Citations (1)

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Application publication date: 20170524