CN106613950A - Production method of special base material for transplanting taro tissue culture seedlings produced by utilizing mushroom residues - Google Patents
Production method of special base material for transplanting taro tissue culture seedlings produced by utilizing mushroom residues Download PDFInfo
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- CN106613950A CN106613950A CN201610908759.9A CN201610908759A CN106613950A CN 106613950 A CN106613950 A CN 106613950A CN 201610908759 A CN201610908759 A CN 201610908759A CN 106613950 A CN106613950 A CN 106613950A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a special base material for transplanting taro tissue culture seedlings produced by utilizing mushroom residues. A formula is prepared from the following components in parts by mass: 60 percent of a fermentation base material, 38 percent of garden soil, 0.5 percent of a microorganism bacterium agent, 0.5 percent of urea and 1 percent of calcium superphosphate, wherein the fermentation base material comprises fermented cattle and sheep manure, the mushroom residues and a base material fermentation bacterium agent strain; the base material fermentation bacterium agent strain is prepared by mixing a microorganism fermentation bacterium agent with corn flour, soybean cake powder or rice bran at the mass ratio of 1 to 10 and the mass ratio of the fermented cattle and sheep manure to the mushroom residues is 1 to 2; the fermented cattle and sheep manure is prepared by mixing cattle and sheep manure and a fermentation agent and the mass ratio of the fermentation agent to the cattle and sheep manure is 0.1 percent to 0.2 percent; the fermentation agent is prepared by mixing a biologic fertilizer fermentation bacterium agent with the soybean cake powder or the rice bran at the mass ratio of 1 to 10. The invention further discloses a production method of the special base material for transplanting the taro tissue culture seedlings produced by utilizing the mushroom residues. The special base material is low in cost and can be used for effectively promoting transplanting and tillering of taro detoxified tissue culture seedlings.
Description
Technical field
The present invention relates to agricultural technology field, more particularly to a kind of production method of dedicated substrate.
Background technology
The area along the Yangtze River in Jiangsu Province possesses high-quality taro distinguishing products more, and its production kind is local high-quality taro place
Farm variety, as the development of high-quality taro industry is paid attention in various places, swashs to the ganoid konjaku taro demand of local high-quality taro farm variety
Increase.But at present the ganoid konjaku taro of Jiangsu Province taro place farm variety is reserved for one's own use based on ganoid konjaku taro with the masses, variety hybriding degradation, long-term dew
Ground plantation, virus accumulation degeneration is serious, needs to purificate and rejuvenate and detoxication and tissue culture various places high-quality taro place farm variety,
The detoxifying fast breeding system of local high-quality taro local characteristic kind is set up one after another in various places.Taro tissue cultural seedlings of free cultivates unit to be needed
Taro plantlet in vitro is carried out after plug transplantation, Plug seedling is supplied to into taro detoxifying fast breeding base, and educated using existing vegetables
Seedling matrix transplants taro plantlet in vitro, and taro plantlet in vitro tiller is slow, is unfavorable for cultivating healthy and strong taro tissue cultural seedlings of free, and cost is also inclined
Height, needs the growth characteristic according to taro detoxication and tissue culture transplantation of seedlings tiller, and a kind of cost of development is relatively low, more efficient promotion taro
The dedicated substrate of tissue cultural seedlings of free tiller.
The content of the invention
The present invention is directed to the deficiencies in the prior art, proposes a kind of low cost, effectively facilitates the transplantation of seedlings of taro detoxication and tissue culture and sends out
Utilization mushroom slag produce taro tissue culture transplantation of seedlings dedicated substrate preparation method.
The present invention is achieved through the following technical solutions technical goal.
The preparation method that a kind of utilization mushroom slag produces taro tissue culture transplantation of seedlings dedicated substrate, it is theed improvement is that：Formula
For (according to the mass fraction)：Fermentation substrate 60%, garden mould 38%, microbial bacterial agent 0.5%, urea 0.5%, calcium superphosphate 1%；
The fermentation substrate includes fermentation cattle and sheep excrement, mushroom slag, substrate fermentation microbial inoculum bacterial classification, and substrate fermentation microbial inoculum bacterial classification is by fermentable
Microbial inoculum presses quality proportioning 1 with corn flour, beancake powder or wheat bran rice bran：10 ratio is mixed, fermentation cattle and sheep excrement, the matter of mushroom slag
Amount proportioning is 1:2；The fermentation cattle and sheep excrement is mixed by cattle and sheep excrement, leavening, and leavening is 0.1 with cattle and sheep excrement quality proportioning
～0.2%；The leavening is by bio-feritlizer fermenting agent with beancake powder or bran according to quality proportioning 1：10 mix.
The preparation method that above-mentioned utilization mushroom slag produces taro tissue culture transplantation of seedlings dedicated substrate, comprises the following steps：
1) cattle and sheep excrement is carried out into fermentation disinfection in 12～17 days, makes fermentation cattle and sheep excrement；
2) mushroom slag, fermentation cattle and sheep excrement, substrate fermentation microbial inoculum bacterial classification are uniformly mixed, makes fermentation substrate；The fermentation ox
Sheep excrement, the quality proportioning of mushroom slag are 1：2, substrate fermentation microbial inoculum bacterial classification is by microbial fermentation inoculum and corn flour, beancake powder or bran
Micromicron chaff presses quality proportioning 1：10 ratio is mixed；
3) dispensing is carried out by following mass fraction：The fermentation substrate 60%, garden mould 38%, microbial bacterial agent 0.5%, urine
Element 0.5% and calcium superphosphate 1%, then full and uniform mixing.
Above-mentioned steps 1) described in ferment cattle and sheep excrement make step：
(1) dry cattle and sheep excrement and be less than 45% to water content；
(2) leavening is modulated：By bio-feritlizer fermenting agent with beancake powder or bran according to quality proportioning 1：10 mixing are equal
It is even, make cattle and sheep manure fermentation agent；
(3) batch mixing：Send out by 0.1～0.2% outfit step (2) the cattle and sheep excrement of cattle and sheep argol weight described in step (1)
Ferment agent, cattle and sheep manure fermentation agent is uniformly admixed in step (1) the cattle and sheep excrement, makes cattle and sheep excrement, leavening batch mixing；
(4) heap fermentation is built：Cattle and sheep excrement, leavening batch mixing described in step (3) are built up into Fei Dui, Fei Dui bottoms are wide by 1.8～2
Rice, high 1.5～1.6 meters, the air vent of the ㎝ of diameter 15～25 is stayed at the center of fertile heap width；The fermentation initial stage is covered with plastic foil
Lid, fermentation temperature is raised to 60 DEG C, takes off film ventilation；
(5) turning：Temperature reaches more than 60 DEG C in fermentation 7～10 days or fertile heap, carries out a turning, by outside fertile heap not
The cattle and sheep excrement of fermentation, leavening batch mixing are translated into inside fertile heap, then build heap fermentation 5～7 days again, make fermentation cattle and sheep excrement.
Above-mentioned steps 2) described in fermentation substrate making step：
1. mushroom Slag treatment：Mushroom slag is smashed, is dried, added water, make water content reach 60～65%；
2. substrate fermentation microbial inoculum bacterial classification is made：Microbial fermentation inoculum is pressed into quality proportioning 1：10 ratio with corn flour,
Beancake powder or wheat bran rice bran, are well mixed, and make substrate fermentation microbial inoculum bacterial classification；
3. dispensing, batch mixing, connect bacterium：Will described in step (5) ferment cattle and sheep excrement, step 1. made by mushroom slag press quality proportioning
1：2 ratio carries out dispensing, and by volume 1m of institute's dispensing3Ratio with the 0.5kg steps 2. substrate fermentation microbial inoculum bacterial classification
Prepare substrate fermentation microbial inoculum bacterial classification；The mushroom slag for being prepared, fermentation cattle and sheep excrement, substrate fermentation microbial inoculum bacterial classification are uniformly mixed, is made
Mushroom slag, fermentation cattle and sheep excrement batch mixing；
4. heap is built：By step, 3. the mushroom slag, fermentation cattle and sheep excrement batch mixing build heap where ventilated, and heap is high by 1.5～2
Rice, 1.8～2 meters of bottom width, 3～5 meters of length；
5. anti-rain：Support one is simple fluffy on the heap that 4. step builds；
6. turning：Turning during 5～10 days or heap Nei Wenduda 60～65 DEG C of fermentation process, builds heap again, ferments 5～7 days；
7. fermentation is completed：In black or pitchy, without any stink, temperature is gradually reduced to 35～40 material naturally in heap
DEG C, fermentation is basically completed；Spread material in heap out, cooling is dried.
The present invention compared with prior art, has the positive effect that：
1. production cost reduces by more than 40%.
2. taro detoxication and tissue culture transplantation of seedlings tiller is remarkably promoted.
3. significantly improve plantlet in vitro to transplant the ganoid konjaku taro yield behind land for growing field crops.
Description of the drawings
Subordinate list 1 is the utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate and vegetable seedling substrate that embodiment 1 makes
Carry out taro plantlet in vitro and send out seedling contrast test table.
Subordinate list 2 is the utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate and vegetable seedling substrate that embodiment 1 makes
Carry out taro plantlet in vitro land for growing field crops Comparative Experiment on Yield table.
Subordinate list 3 is the utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate and vegetable seedling substrate that embodiment 2 makes
Carry out taro plantlet in vitro and send out seedling contrast test table.
Subordinate list 4 is the utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate and vegetable seedling substrate that embodiment 2 makes
Carry out taro plantlet in vitro land for growing field crops Comparative Experiment on Yield table.
Specific embodiment
With reference to embodiment, subordinate list, the invention will be further described.
Embodiment 1
1) fermentation cattle and sheep excrement is made, its making step is；
(1) dry cattle and sheep excrement and be less than 45% to water content；
(2) cattle and sheep manure fermentation agent is modulated：By bio-feritlizer fermenting agent with beancake powder or bran according to quality proportioning 1：10
It is well mixed, makes cattle and sheep manure fermentation agent；
(3) batch mixing：By 0.1% outfit step (2) the cattle and sheep manure fermentation agent of cattle and sheep argol weight described in step (1),
Cattle and sheep manure fermentation agent is uniformly admixed in step (1) the cattle and sheep excrement, cattle and sheep excrement, leavening batch mixing is made；
(4) heap fermentation is built：Cattle and sheep excrement, leavening batch mixing described in step (3) are built up into Fei Dui, Fei Dui bottoms are wide by 1.8～2
Rice, high 1.5～1.6 meters, the air vent of the ㎝ of diameter 15～25 is stayed at the center of fertile heap width；The fermentation initial stage is covered with plastic foil
Lid, fermentation temperature is raised to 60 DEG C, takes off film ventilation；
(5) turning：Temperature reaches more than 60 DEG C in fermentation 7 days or fertile heap, carries out a turning, will not ferment outside fertile heap
Cattle and sheep excrement, leavening batch mixing translate into inside fertile heap, then build again heap ferment 5 days, make fermentation cattle and sheep excrement.
2) fermentation substrate is made, its making step is；
1. mushroom Slag treatment：Mushroom slag is smashed, is dried, added water, make water content reach 60%；
2. substrate fermentation microbial inoculum bacterial classification is made：Microbial fermentation inoculum is pressed into quality proportioning 1：10 ratio with corn flour,
Beancake powder or wheat bran rice bran, are well mixed, and make substrate fermentation microbial inoculum bacterial classification；
3. dispensing, batch mixing, connect bacterium：Will described in step (5) ferment cattle and sheep excrement, step 1. made by mushroom slag press quality proportioning
1：2 ratio carries out dispensing, and by volume 1m of institute's dispensing3Ratio with the 0.5kg steps 2. substrate fermentation microbial inoculum bacterial classification
Prepare substrate fermentation microbial inoculum bacterial classification；The mushroom slag for being prepared, fermentation cattle and sheep excrement, substrate fermentation microbial inoculum bacterial classification are uniformly mixed, is made
Mushroom slag, fermentation cattle and sheep excrement batch mixing；
4. heap is built：By step, 3. the mushroom slag, fermentation cattle and sheep excrement batch mixing build heap where ventilated, and heap is high by 1.5～2
Rice, 1.8～2 meters of bottom width, 3～5 meters of length；
5. anti-rain：Support one is simple fluffy on the heap that 4. step builds；
6. turning：Turning during 5 days or heap Nei Wenduda 60 DEG C of fermentation process, builds heap again, ferments 5 days；
7. fermentation is completed：In black or pitchy, without any stink, temperature is gradually reduced to 35 DEG C material naturally in heap,
Fermentation is basically completed；Spread material in heap out, cooling is dried.
3) dispensing is carried out by following mass fraction：The fermentation substrate 60%, garden mould 38%, microbial bacterial agent 0.5%, urine
Element 0.5% and calcium superphosphate 1%, then full and uniform mixing, that is, make the utilization mushroom slag production taro tissue culture transplantation of seedlings special
Use matrix.
In the utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate that the present embodiment makes, as the mushroom of primary raw material
The raw materials such as slag, cattle and sheep excrement, garden mould are discarded object or gather materials on the spot, cheap, the utilization mushroom slag production that the present embodiment makes
Taro tissue culture transplantation of seedlings dedicated substrate cost of manufacture be less than 576 yuan/ton, and the market price of vegetable seedling substrate be 1000 yuan/
Ton, therefore, production cost reduces by 44%.
The utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate made using the present embodiment is entered with vegetable seedling substrate
Row taro plantlet in vitro sends out seedling contrast test, studies the impact of the growth of cereal crop seedlings that two kinds of matrix are cultivated the transplantation of seedlings of taro tissue culture, as a result arranges
In subordinate list 1.Utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate and the vegetable seedling substrate made using the present embodiment with
Compare, survival rate is higher by 2%, fast 0.121 leaf of leaf emergence rate/d, plant height raising 17.2%, many 1.9g of cauline leaf fresh weight, cauline leaf dry weight
Many 0.112g.Result of the test shows：The utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate made using the present embodiment is shown
Write and promote taro detoxication and tissue culture transplantation of seedlings tiller, be that the healthy and strong plantlet in vitro of culture lays the foundation.
Subordinate list 1
The utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate made using the present embodiment is entered with vegetable seedling substrate
Row taro plantlet in vitro land for growing field crops Comparative Experiment on Yield, is as a result listed in subordinate list 2.The utilization mushroom slag production taro made using the present embodiment
Tissue culture transplantation of seedlings dedicated substrate is with vegetable seedling substrate compared with, and survival rate is high, sends out seedling speed soon, and the sub- taro number for ultimately forming is more
1.7/plant, output increased 29.96g/ strains.Result of the test shows：The utilization mushroom slag production taro group made using the present embodiment
Training transplantation of seedlings dedicated substrate significantly improves plantlet in vitro and transplants the ganoid konjaku taro yield behind land for growing field crops.
Subordinate list 2
Embodiment 2
1) fermentation cattle and sheep excrement is made, its making step is；
(1) dry cattle and sheep excrement and be less than 45% to water content；
(2) cattle and sheep manure fermentation agent is modulated：By bio-feritlizer fermenting agent with beancake powder or bran according to quality proportioning 1：10
It is well mixed, makes cattle and sheep manure fermentation agent；
(3) batch mixing：By 0.2% outfit step (2) the cattle and sheep manure fermentation agent of cattle and sheep argol weight described in step (1),
Cattle and sheep manure fermentation agent is uniformly admixed in step (1) the cattle and sheep excrement, cattle and sheep excrement, leavening batch mixing is made；
(4) heap fermentation is built：Cattle and sheep excrement, leavening batch mixing described in step (3) are built up into Fei Dui, Fei Dui bottoms are wide by 1.8～2
Rice, high 1.5～1.6 meters, the air vent of the ㎝ of diameter 15～25 is stayed at the center of fertile heap width；The fermentation initial stage is covered with plastic foil
Lid, fermentation temperature is raised to 60 DEG C, takes off film ventilation；
(5) turning：Temperature reaches more than 60 DEG C in fermentation 10 days or fertile heap, carries out a turning, will not send out outside fertile heap
The cattle and sheep excrement of ferment, leavening batch mixing are translated into inside fertile heap, then build heap fermentation 7 days again, make fermentation cattle and sheep excrement.
2) fermentation substrate is made, its making step is；
1. mushroom Slag treatment：Mushroom slag is smashed, is dried, added water, make water content reach 65%；
2. substrate fermentation microbial inoculum bacterial classification is made：Microbial fermentation inoculum is pressed into quality proportioning 1：10 ratio with corn flour,
Beancake powder or wheat bran rice bran, are well mixed, and make substrate fermentation microbial inoculum bacterial classification；
3. dispensing, batch mixing, connect bacterium：Will described in step (5) ferment cattle and sheep excrement, step 1. made by mushroom slag press quality proportioning
1：2 ratio carries out dispensing, and by volume 1m of institute's dispensing3Ratio with the 0.5kg steps 2. substrate fermentation microbial inoculum bacterial classification
Prepare substrate fermentation microbial inoculum bacterial classification；The mushroom slag for being prepared, fermentation cattle and sheep excrement, substrate fermentation microbial inoculum bacterial classification are uniformly mixed, is made
Mushroom slag, fermentation cattle and sheep excrement batch mixing；
4. heap is built：By step, 3. the mushroom slag, fermentation cattle and sheep excrement batch mixing build heap where ventilated, and heap is high by 1.5～2
Rice, 1.8～2 meters of bottom width, 3～5 meters of length；
5. anti-rain：Support one is simple fluffy on the heap that 4. step builds；
6. turning：Turning during 10 days or heap Nei Wenduda 65 DEG C of fermentation process, builds heap again, ferments 7 days；
7. fermentation is completed：In black or pitchy, without any stink, temperature is gradually reduced to 40 DEG C material naturally in heap,
Fermentation is basically completed；Spread material in heap out, cooling is dried.
3) dispensing is carried out by following mass fraction：The fermentation substrate 60%, garden mould 38%, microbial bacterial agent 0.5%, urine
Element 0.5% and calcium superphosphate 1%, then full and uniform mixing, that is, make the utilization mushroom slag production taro tissue culture transplantation of seedlings special
Use matrix.
In the utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate that the present embodiment makes, as the mushroom of primary raw material
The raw materials such as slag, cattle and sheep excrement, garden mould are discarded object or gather materials on the spot, cheap, the utilization mushroom slag production that the present embodiment makes
Taro tissue culture transplantation of seedlings dedicated substrate cost of manufacture be less than 579 yuan/ton, and the market price of vegetable seedling substrate be 1000 yuan/
Ton, therefore, production cost reduces by 41%.
The utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate made using the present embodiment is entered with vegetable seedling substrate
Row taro plantlet in vitro sends out seedling contrast test, studies the impact of the growth of cereal crop seedlings that two kinds of matrix are cultivated the transplantation of seedlings of taro tissue culture, as a result arranges
In subordinate list 3.Utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate and the vegetable seedling substrate made using the present embodiment with
Compare, survival rate is higher by 2.08%, fast 0.124 leaf of leaf emergence rate/d, plant height raising 17.4%, many 1.93g of cauline leaf fresh weight, cauline leaf
The many 0.115g of dry weight.Result of the test shows：The special base of utilization mushroom slag production taro tissue culture transplantation of seedlings made using the present embodiment
Matter remarkably promotes taro detoxication and tissue culture transplantation of seedlings tiller, is that the healthy and strong plantlet in vitro of culture lays the foundation.
Subordinate list 3
The utilization mushroom slag production taro tissue culture transplantation of seedlings dedicated substrate made using the present embodiment is entered with vegetable seedling substrate
Row taro plantlet in vitro land for growing field crops Comparative Experiment on Yield, is as a result listed in subordinate list 4.The utilization mushroom slag production taro made using the present embodiment
Tissue culture transplantation of seedlings dedicated substrate is with vegetable seedling substrate compared with, and survival rate is high, sends out seedling speed soon, and the sub- taro number for ultimately forming is more
1.9/plant, output increased 30.01g/ strains.Result of the test shows：The utilization mushroom slag production taro group made using the present embodiment
Training transplantation of seedlings dedicated substrate significantly improves plantlet in vitro and transplants the ganoid konjaku taro yield behind land for growing field crops.
Subordinate list 4
Claims (4)
- It is 1. a kind of to produce taro tissue culture transplantation of seedlings dedicated substrate using mushroom slag, it is characterised in that：Fill a prescription and be（According to the mass fraction）： Fermentation substrate 60%, garden mould 38%, microbial bacterial agent 0.5%, urea 0.5%, calcium superphosphate 1%；The fermentation substrate includes fermentation ox Sheep excrement, mushroom slag, substrate fermentation microbial inoculum bacterial classification, substrate fermentation microbial inoculum bacterial classification by microbial fermentation inoculum and corn flour, beancake powder or Wheat bran rice bran presses quality proportioning 1：10 ratio is mixed, and the cattle and sheep excrement that ferments, the quality proportioning of mushroom slag are 1:2；The fermentation Cattle and sheep excrement is mixed by cattle and sheep excrement, leavening, and leavening is 0.1～0.2% with cattle and sheep excrement quality proportioning；The leavening by Bio-feritlizer fermenting agent is with beancake powder or bran according to quality proportioning 1：10 mix.
- 2. the preparation method for producing taro tissue culture transplantation of seedlings dedicated substrate using mushroom slag described in claim 1, comprises the following steps：1）Cattle and sheep excrement is carried out into fermentation disinfection in 12～17 days, fermentation cattle and sheep excrement is made；2）Mushroom slag, fermentation cattle and sheep excrement, substrate fermentation microbial inoculum bacterial classification are uniformly mixed, fermentation substrate is made；The fermentation cattle and sheep excrement, The quality proportioning of mushroom slag is 1：2, substrate fermentation microbial inoculum bacterial classification is by microbial fermentation inoculum and corn flour, beancake powder or wheat bran rice bran By quality proportioning 1：10 ratio is mixed；3）Dispensing is carried out by following mass fraction：The fermentation substrate 60%, garden mould 38%, microbial bacterial agent 0.5%, urea 0.5% With calcium superphosphate 1%, then full and uniform mixing.
- 3. the preparation method that utilization mushroom slag according to claim 2 produces taro tissue culture transplantation of seedlings dedicated substrate, its feature It is：Step 1）Described in ferment cattle and sheep excrement make step：（1）Dry cattle and sheep excrement and be less than 45% to water content；（2）Modulation leavening：By bio-feritlizer fermenting agent with beancake powder or bran according to quality proportioning 1：10 are well mixed, system Into cattle and sheep manure fermentation agent；（3）Batch mixing：By step（1）Described in cattle and sheep argol weight 0.1～0.2% outfit step（2）The cattle and sheep manure fermentation agent, Step is uniformly admixed in cattle and sheep manure fermentation agent（1）In the cattle and sheep excrement, cattle and sheep excrement, leavening batch mixing are made；（4）Build heap fermentation：By step（3）Described in cattle and sheep excrement, leavening batch mixing build up Fei Dui, Fei Dui bottoms are wide 1.8～2 meters, High 1.5～1.6 meters, the air vent of the ㎝ of diameter 15～25 is stayed at the center of fertile heap width；The fermentation initial stage is covered with plastic foil, is sent out Ferment temperature is raised to 60 DEG C, takes off film ventilation；（5）Turning：Temperature reaches more than 60 DEG C in fermentation 7～10 days or fertile heap, carries out a turning, will not ferment outside fertile heap Cattle and sheep excrement, leavening batch mixing translate into inside fertile heap, then build again heap ferment 5～7 days, make fermentation cattle and sheep excrement.
- 4. the preparation method that utilization mushroom slag according to claim 3 produces taro tissue culture transplantation of seedlings dedicated substrate, its feature It is：Step 2）Described in fermentation substrate making step：1. mushroom Slag treatment：Mushroom slag is smashed, is dried, added water, make water content reach 60～65%；2. substrate fermentation microbial inoculum bacterial classification is made：Microbial fermentation inoculum is pressed into quality proportioning 1：10 ratio is with corn flour, soya-bean cake Powder or wheat bran rice bran, are well mixed, and make substrate fermentation microbial inoculum bacterial classification；3. dispensing, batch mixing, connect bacterium：By step（5）Described in ferment cattle and sheep excrement, step 1. made by mushroom slag press quality proportioning 1：2 Ratio carry out dispensing, and by the m of volume 1 of institute's dispensing3Ratio with the 0.5kg steps 2. substrate fermentation microbial inoculum bacterial classification is accurate Standby substrate fermentation microbial inoculum bacterial classification；The mushroom slag for being prepared, fermentation cattle and sheep excrement, substrate fermentation microbial inoculum bacterial classification are uniformly mixed, mushroom is made Slag, fermentation cattle and sheep excrement batch mixing；4. heap is built：By step, 3. the mushroom slag, fermentation cattle and sheep excrement batch mixing build heap where ventilated, and heap is high 1.5～2 meters, 1.8～2 meters of bottom width, 3～5 meters of length；5. anti-rain：Support one is simple fluffy on the heap that 4. step builds；6. turning：Turning during 5～10 days or heap Nei Wenduda 60～65 DEG C of fermentation process, builds heap again, ferments 5～7 days；7. fermentation is completed：In black or pitchy, without any stink, temperature is gradually reduced to 35～40 DEG C material naturally in heap, Fermentation is basically completed；Spread material in heap out, cooling is dried.
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| CN110150095A (en) * | 2019-06-28 | 2019-08-23 | 江苏省农业科学院泰州农科所 | A kind of method for culturing seedlings of taro selenium-rich organic seedling medium and preparation method thereof and selenium-rich taro |
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