CN104430547A - Biological intelligent bacterium block formula for controlling soil-borne diseases and preparation method thereof - Google Patents
Biological intelligent bacterium block formula for controlling soil-borne diseases and preparation method thereof Download PDFInfo
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- 241000894006 Bacteria Species 0.000 title claims abstract description 61
- 201000010099 disease Diseases 0.000 title claims abstract description 20
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 241000196324 Embryophyta Species 0.000 claims abstract description 12
- 241000918585 Pythium aphanidermatum Species 0.000 claims abstract description 12
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 8
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 7
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims abstract description 7
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 7
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims abstract description 7
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000223218 Fusarium Species 0.000 claims abstract description 6
- 241000187395 Streptomyces microflavus Species 0.000 claims abstract description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 4
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- 244000241235 Citrullus lanatus Species 0.000 claims abstract 2
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- 239000007788 liquid Substances 0.000 claims description 27
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- 238000005498 polishing Methods 0.000 claims description 8
- 241001088165 Streptomyces microaureus Species 0.000 claims description 7
- 240000008574 Capsicum frutescens Species 0.000 claims description 6
- 239000001390 capsicum minimum Substances 0.000 claims description 6
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- 229920000084 Gum arabic Polymers 0.000 claims description 4
- HYVABZIGRDEKCD-UHFFFAOYSA-N N(6)-dimethylallyladenine Chemical compound CC(C)=CCNC1=NC=NC2=C1N=CN2 HYVABZIGRDEKCD-UHFFFAOYSA-N 0.000 claims description 4
- 241000728344 Streptomyces hygrospinosus Species 0.000 claims description 4
- 235000010489 acacia gum Nutrition 0.000 claims description 4
- 239000000205 acacia gum Substances 0.000 claims description 4
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- MZYRDLHIWXQJCQ-YZOKENDUSA-L potassium alginate Chemical compound [K+].[K+].O1[C@@H](C([O-])=O)[C@@H](OC)[C@H](O)[C@H](O)[C@@H]1O[C@@H]1[C@@H](C([O-])=O)O[C@@H](O)[C@@H](O)[C@H]1O MZYRDLHIWXQJCQ-YZOKENDUSA-L 0.000 claims description 4
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- 235000015278 beef Nutrition 0.000 claims description 3
- 230000003449 preventive effect Effects 0.000 claims description 3
- 230000001225 therapeutic effect Effects 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 21
- 238000001035 drying Methods 0.000 abstract description 8
- 230000003405 preventing effect Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 3
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- 239000006002 Pepper Substances 0.000 abstract 1
- 235000016761 Piper aduncum Nutrition 0.000 abstract 1
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- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a biological intelligent living body bacterium block for controlling soil-borne diseases of plants. According to the biological intelligent bacterium block, the following three strains are adopted: ACCC40033: Streptomyceshygrospinosusvar.beijingensis Taoetal, ACC40060: Streptomycesmicroflavus (Yanetal.) Yanetal. and ACCC11112: Bacillussubtilis (Ehrenberg)Cohn. The preparation method comprises the following steps: preparing yeast powder of each strain by fermenting and drying, mixing with a carrier auxiliary material in proportion, and pressing to form the product. The biological intelligent bacterium block can be used for growing seedlings of multiple vegetables, flowers and plants and commercial crops, can prevent the injury of the soil-borne diseases on the crops at the seedling stage, also can achieve an excellent effect of preventing the soil-borne diseases of plants after the plants are transplanted into a field or a greenhouse, and especially has an excellent effect of controlling the fusarium wilt of cucumber, damping off of pepper and damping-off of watermelons.
Description
Technical field
The present invention relates to a kind of nursery bacterium block, particularly relate to a kind of formula and preparation method thereof that can be used for the living body biological intelligent bacterium block preventing and treating plant soil-borne diseases.
Background technology
Soil-borne disease is the disease that the pathogen in soil causes from the root of crop or basal part of stem infringement crop when condition is suitable for, its pathogen mainly carries out propagation harm by soil organic fertilizer material, irrigation water or natural flowing water, endangers serious plot and can make crop failure 30%-50%.
Why more and more serious soil-borne disease is, difficult anti-refractory, trace it to its cause is exactly that the pathogen of soil-borne disease is generally multiparasitism microorganism, a kind of pathogen just can infect several and even hundreds of crops and weeds, and the method making traditional crop rotation, Fruit variety and improvement planting type etc. administer soil-borne disease has significant limitation.
The present invention is with ACCC40033: S.hygrospinous var.Beijingensis
streptomyces hygrospinosus var.beigingenisTao et al, ACCC40060: streptomyces microaureus
streptomyces microflavus(Yan et al.) Yan et al. and ACCC11112: bacillus subtilis
bacillus subtilis(Ehrenberg) these 3 bacterial classifications of Cohn(all obtain and are purchased from Chinese agriculture Microbiological Culture Collection administrative center) send out a dusty yeast be the nucleus preventing and treating soil-borne disease, be aided with natural carrier material (turfy soil, coconut palm chaff, sugar slag), growth-promoting agent (oxyenadenine, enadenine), coagulating agent (polyacrylamide gel), adhesive (gum arabic powder), protectant (potassium alginate), middle bandwidth 2cm is pressed into special tablet press machine, be cylindrical bacterium block (the diameter 4.5cm of 1cm aperture deeply, high 2.5cm), obtained biological intelligence bacterium block of the present invention.
Biological intelligence bacterium block of the present invention; the antibiotic produced not only makes inhibitory action to the pathogen of multiple soil-borne disease; lethal effect can also be produced to the pathogenic microorganism of sensitivity; simultaneously the bacterial classification of biological intelligence bacterium block is on a good wicket producing in the competition in ecological site with pathogenic microorganism, protects the growth of crop better.
Product of the present invention is applicable to various vegetables, flowers, the nursery of economic crops and transplanting, the generation of plant soil-borne diseases not only can be prevented in seedling stage, and after being transplanted to field or greenhouse, still can play extraordinary preventive and therapeutic effect to the soil-borne disease of plant.Transplant and can reach more than 90% to the soil-borne disease preventive effect of the plants such as watermelon damping off, capsicum damping off, cucumber fusarium axysporum after 15 days, the lasting period reaches 2-3 month; And there is the feature being conducive to the ecological balance such as nontoxic to person poultry safety, free from environmental pollution, meet the development strategy of country to the ecological agriculture, achieve the high unity of economic benefit and ecological benefits.
Summary of the invention
Strains A CCC40033 is S.hygrospinous var.Beijingensis
streptomyces hygrospinosus var.beigingenisTao et al; Strains A CCC40060 is streptomyces microaureus
streptomyces microflavus(Yan et al.) Yan et al.; Strains A CCC11112 is bacillus subtilis
bacillus subtilis(Ehrenberg) Cohn; Above-mentioned 3 kinds of bacterial strains are all purchased from Chinese agriculture Microbiological Culture Collection administrative center.
Biological intelligence bacterium block of the present invention is adopted and is prepared with the following method.
Step one, preparation send out dusty yeast.
(1) for preparing strains A CCC40033 sends out dusty yeast: liquid fermentation medium formula: KNO
3-1.2g, K
2hPO
4-0.54g, NaCl-0.55g, FeSO
47H
2o-0.01g, soluble starch-25g, MgSO
47H
2o-0.6g, oxyenadenine-0.5g, enadenine-0.1g, agar-0.25g, pH value is adjusted to 7.2, and clear water polishing is to 1000g.Configure the amount of required fermentation medium according to the above ratio, then bacterial strain accessed in liquid fermentation medium under aseptic condition, under 30 DEG C of conditions on shaking table shaken cultivation after 72 hours, (living bacteria count all reaches 3.0 × 10
9cfu/ml) obtained first order seed, then by cultured first order seed in 3% ratio, access is equipped with the sterilizing for preparing according to the above ratio and is carried out fermenting and producing in cooled fermentation tank, fermented and cultured temperature 30 DEG C, throughput start 1:1 (v. v. m), cultivate to increase to after 24 hours 1:1.5 (v. v. m), be down to after 48 hours 1:1.2 (v. v. m), ferment complete to 72 hours fermentation, ACCC40033 in microscopy zymotic fluid: it is qualified bacterium liquid that the bacterium amount of S.hygrospinous var.Beijingensis reaches 3,000,000,000 cfu/ml, finally in the ratio of 7:3, bacterium liquid and carrier are stirred, put into temperature and carry out drying at the pneumatic drier of about 90 ± 1 DEG C, finally obtain a qualified dusty yeast.
(2) for preparing ACCC40060 sends out dusty yeast: liquid fermentation medium formula: KNO
3-1.2g, K
2hPO
4-0.54g, NaCl-0.55g, FeSO
47H
2o-0.01g, soluble starch-25g, MgSO
47H
2o-0.6g, oxyenadenine-0.5g, enadenine-0.1g, agar-0.25g, pH value is adjusted to 7.2, and clear water polishing is to 1000g.Configure the amount of required fermentation medium according to the above ratio, then bacterial strain accessed in liquid fermentation medium under aseptic condition, under 30 DEG C of conditions on shaking table shaken cultivation after 72 hours, (living bacteria count all reaches 3.0 × 10
9cfu/ml) obtained first order seed; Then by cultured first order seed in 3% ratio, access is equipped with the sterilizing for preparing according to the above ratio and is carried out fermenting and producing in cooled fermentation tank, fermented and cultured temperature 30 DEG C, throughput starts 1: 1.2 (v. v. m), cultivate to increase to after 24 hours 1:1.4 (v. v. m), after 48 hours, continue to increase to 1: 1.6 (v. v. m).Ferment complete to 72 hours fermentation, ACCC40060 in microscopy zymotic fluid: it is that bacterium liquid and carrier finally stir in the ratio of 7:3 by qualified bacterium liquid that the bacterium amount of streptomyces microaureus reaches 3,000,000,000 cfu/ml, put into temperature and carry out drying at the pneumatic drier of about 90 ± 1 DEG C, finally obtain qualified sending out dusty yeast.
(3) for preparing ACCC11112 sends out dusty yeast: liquid fermentation medium formula: peptone-5.3g, NaCl-5.8g, MnSO
4h
2o-0.004g, oxyenadenine-0.4g, enadenine-0.2g, beef extract-3.3g, agar-0.25g, pH value is adjusted to 7.0, and clear water polishing is to 1000g.Configure the amount of required fermentation medium according to the above ratio, then bacterial strain accessed in liquid fermentation medium under aseptic condition, under 30 DEG C of conditions on shaking table shaken cultivation after 72 hours, (living bacteria count all reaches 3.0 × 10
9cfu/ml) obtained first order seed; Then by cultured first order seed in 3% ratio, access is equipped with the sterilizing for preparing according to the above ratio and is carried out fermenting and producing in cooled fermentation tank, fermented and cultured temperature 30 DEG C, throughput starts 1: 1 (v. v. m), cultivate to increase to after 24 hours 1:1.5 (v. v. m), after 48 hours, continue to increase to 1: 1.2 (v. v. m).Ferment complete to 72 hours fermentation, ACCC11112 in microscopy zymotic fluid: it is qualified bacterium liquid that the bacterium amount of bacillus subtilis reaches 3,000,000,000 cfu/ml, finally in the ratio of 7:3, bacterium liquid and carrier are stirred, put into temperature and carry out drying at the pneumatic drier of about 90 ± 1 DEG C, finally obtain qualified sending out dusty yeast.
The above bacterium liquid and carrier stir in drying, and carrier is the calcium carbonate of 6:4 ratio and pulls open powder mixture.
The preparation of step 2, biological intelligence bacterium block.
By weight percentage the ratio of turfy soil 58.6%, coconut palm chaff 15%, sugared slag 15%, oxyenadenine 0.2%, enadenine 0.2%, polyacrylamide gel 2%, gum arabic powder 1%, potassium alginate 8% is mixed and make carrier mass.Then by the carrier mass configured and a dusty yeast of strains A CCC40033, the dusty yeast sending out dusty yeast, strains A CCC11112 of strains A CCC40060, puddle evenly according to percentage by weight 10:0.4-1:0.4-1:0.4-1, middle bandwidth 2cm, dark cylindrical bacterium block (the diameter 4.5cm for 1cm aperture is pressed into special tablet press machine, high 2.5cm), obtained biological intelligence bacterium block of the present invention.
Embodiment
In order to make object of the present invention, technical scheme clearly understands, be described, but the present invention is not limited to these instantiations with following instantiation.
Embodiment 1: prepare biological intelligence bacterium block of the present invention.
The bacterial strain used: strains A CCC40033 is S.hygrospinous var.Beijingensis
streptomyces hygrospinosus var.beigingenisTao et al, strains A CCC40060 is streptomyces microaureus
streptomyces microflavus(Yan et al.) Yan et al., strains A CCC11112: bacillus subtilis
bacillus subtilis(Ehrenberg) Cohn, these 3 kinds of bacterial strains all obtain purchased from Chinese agriculture Microbiological Culture Collection administrative center.
First, for preparing strains A CCC40033 sends out dusty yeast: liquid fermentation medium formula: KNO
3-1.2g, K
2hPO
4-0.54g, NaCl-0.55g, FeSO
47H
2o-0.01g, soluble starch-25g, MgSO
47H
2o-0.6g, oxyenadenine-0.5g, enadenine-0.1g, agar-0.25g, pH value is adjusted to 7.2, and clear water polishing is to 1000g.Configure the amount of required fermentation medium according to the above ratio, then bacterial strain accessed in liquid fermentation medium under aseptic condition, under 30 DEG C of conditions on shaking table shaken cultivation after 72 hours, (living bacteria count all reaches 3.0 × 10
9cfu/ml) obtained first order seed, then by cultured first order seed in 3% ratio, access is equipped with the sterilizing for preparing according to the above ratio and is carried out fermenting and producing in cooled fermentation tank, fermented and cultured temperature 30 DEG C, throughput start 1:1 (v. v. m), cultivate to increase to after 24 hours 1:1.5 (v. v. m), be down to after 48 hours 1:1.2 (v. v. m), ferment complete to 72 hours fermentation, ACCC40033 in microscopy zymotic fluid: it is qualified bacterium liquid that the bacterium amount of S.hygrospinous var.Beijingensis reaches 3,000,000,000 cfu/ml, finally in the ratio of 7:3, bacterium liquid and carrier are stirred, put into temperature and carry out drying at the pneumatic drier of about 90 ± 1 DEG C, finally obtain a qualified dusty yeast.
Secondly, a dusty yeast of preparation ACCC40060: liquid fermentation medium formula: KNO
3-1.2g, K
2hPO
4-0.54g, NaCl-0.55g, FeSO
47H
2o-0.01g, soluble starch-25g, MgSO
47H
2o-0.6g, oxyenadenine-0.5g, enadenine-0.1g, agar-0.25g, pH value is adjusted to 7.2, and clear water polishing is to 1000g.Configure the amount of required fermentation medium according to the above ratio, then bacterial strain accessed in liquid fermentation medium under aseptic condition, under 30 DEG C of conditions on shaking table shaken cultivation after 72 hours, (living bacteria count all reaches 3.0 × 10
9cfu/ml) obtained first order seed; Then by cultured first order seed in 3% ratio, access is equipped with the sterilizing for preparing according to the above ratio and is carried out fermenting and producing in cooled fermentation tank, fermented and cultured temperature 30 DEG C, throughput starts 1: 1.2 (v. v. m), cultivate to increase to after 24 hours 1:1.4 (v. v. m), after 48 hours, continue to increase to 1: 1.6 (v. v. m).Ferment complete to 72 hours fermentation, ACCC40060 in microscopy zymotic fluid: it is that bacterium liquid and carrier finally stir in the ratio of 7:3 by qualified bacterium liquid that the bacterium amount of streptomyces microaureus reaches 3,000,000,000 cfu/ml, put into temperature and carry out drying at the pneumatic drier of about 90 ± 1 DEG C, finally obtain qualified sending out dusty yeast.
Again, for preparing ACCC11112 sends out dusty yeast: liquid fermentation medium formula: peptone-5.3g, NaCl-5.8g, MnSO
4h
2o-0.004g, oxyenadenine-0.4g, enadenine-0.2g, beef extract-3.3g, agar-0.25g, pH value is adjusted to 7.0, and clear water polishing is to 1000g.Configure the amount of required fermentation medium according to the above ratio, then bacterial strain accessed in liquid fermentation medium under aseptic condition, under 30 DEG C of conditions on shaking table shaken cultivation after 72 hours, (living bacteria count all reaches 3.0 × 10
9cfu/ml) obtained first order seed; Then by cultured first order seed in 3% ratio, access is equipped with the sterilizing for preparing according to the above ratio and is carried out fermenting and producing in cooled fermentation tank, fermented and cultured temperature 30 DEG C, throughput starts 1: 1 (v. v. m), cultivate to increase to after 24 hours 1:1.5 (v. v. m), after 48 hours, continue to increase to 1: 1.2 (v. v. m).Ferment complete to 72 hours fermentation, ACCC11112 in microscopy zymotic fluid: it is qualified bacterium liquid that the bacterium amount of bacillus subtilis reaches 3,000,000,000 cfu/ml, finally in the ratio of 7:3, bacterium liquid and carrier are stirred, put into temperature and carry out drying at the pneumatic drier of about 90 ± 1 DEG C, finally obtain qualified sending out dusty yeast.
Finally, take turfy soil 5.86kg, coconut palm chaff 1.5kg, sugar slag 1.5kg, oxyenadenine 20g, enadenine 20g, polyacrylamide gel 200g, gum arabic powder 100g, potassium alginate 800g, strains A CCC40033 sends out dusty yeast 500g, strains A CCC40060 sends out dusty yeast 500g, the dusty yeast 500g mix and blend of sending out of strains A CCC11112 makes the carrier mass that total amount is 11.5kg, put into special tablet press machine and be pressed into middle bandwidth 2cm, be cylindrical bacterium block (the diameter 4.5cm of 1cm aperture deeply, high 2.5cm), obtained biological intelligence bacterium block of the present invention.
Antagonistic effect: by strains A CCC40033, strains A CCC40060 and strains A CCC11112 inoculated and cultured on high Gause I solid culture medium, nutrient agar medium and Gause I and nutrient mixed solid state cuture base respectively, place spaced 3cm at 3, each combination carries out intersection antagonistic effect in triplicate, result shows to there is not antagonism between three, can syntrophism.
Embodiment 2: the biologically active measuring biological intelligence bacterium block of the present invention.
Obtain by preparation method of the present invention: only contain the nursery bacterium block of strains A CCC40033, only contain the nursery bacterium block of strains A CCC40060, only containing nursery bacterium block and the biological intelligence bacterium block of the present invention of strains A CCC11112.
Also being finally transplanted to the soil-borne diseases such as cucumber fusarium axysporum, capsicum damping off and watermelon damping off respectively there is serious greenhouse or plot to carry out nursery to cucumber, capsicum and watermelon respectively with above 4 kinds of obtained bacterium blocks, within 15 days, observes the incidence of disease of cucumber fusarium axysporum, capsicum damping off and watermelon damping off afterwards.
Table 1 is that four kinds of bacterium blocks test the control efficiency after 15 days for preventing and treating cucumber fusarium axysporum.
| Process title | ACCC40033 | ACCC40060 | ACCC11112 | Biological intelligence bacterium block |
| Control efficiency | 44.5% | 68.1% | 64.3% | 92.0% |
Table 2 is the control efficiency that four kinds of bacterium blocks are tested for preventing and treating capsicum damping off 15 days.
| Process title | ACCC40033 | ACCC40060 | ACCC11112 | Biological intelligence bacterium block |
| Control efficiency | 69.1% | 78.2% | 61.5% | 90.7% |
Table 3 is the control efficiency that four kinds of bacterium blocks are tested for preventing and treating watermelon damping off 15 days.
| Process title | ACCC40033 | ACCC40060 | ACCC11112 | Biological intelligence bacterium block |
| Control efficiency | 62.4% | 70.2% | 67.5% | 93.7% |
As can be seen from table 1, table 2 and table 3 data, the control efficiency preparing biological intelligence bacterium block after obviously not having three kinds of bacterial strain mixtures by the control efficiency that single bacterial strain prepares bacterium block is good, illustrate these three kinds of bacterial strains by a certain percentage mixture can play together and to have complementary advantages between microorganism and the effect of Synergistic.
A kind of preparation method preventing and treating the living body biological intelligent bacterium block of plant soil-borne diseases of the present invention is described by specific embodiment.Those skilled in the art can use for reference the links such as the suitable feed change of content of the present invention, process conditions and realize other object corresponding, its relevant change does not all depart from content of the present invention, all similar replacements and change will become apparent to those skilled in the art that and be all deemed to be included within scope of the present invention.
Claims (6)
1. prevent and treat a living body biological intelligent bacterium block for plant soil-borne diseases, it is characterized in that: described biological intelligence bacterium block adopts 3 kinds of strains A CCC40033: S.hygrospinous var.Beijingensis
streptomyces hygrospinosus var.beigingenisTao et al, ACCC40060: streptomyces microaureus
streptomyces microflavus(Yan et al.) Yan et al. and ACCC11112: bacillus subtilis
bacillus subtilis(Ehrenberg) Cohn, mixes compacting in specific proportions with carrier auxiliary material mixture and forms after the female powder of fermentation dried obtained 3 kinds of bacterial strains.
2. ACCC40033 according to claim 1: S.hygrospinous var.Beijingensis and ACCC40060: the liquid fermentation formula of streptomyces microaureus is: KNO
3-1.2g, K
2hPO
4-0.54g, NaCl-0.55g, FeSO
47H
2o-0.01g, soluble starch-25g, MgSO
47H
2o-0.6g, oxyenadenine-0.5g, enadenine-0.1g, agar-0.25g, pH value is adjusted to 7.2, and clear water polishing is to 1000g.
3. ACCC11112 according to claim 1: the liquid fermentation formula of bacillus subtilis is: peptone-5.3g, NaCl-5.8g, MnSO
4h
2o-0.004g, oxyenadenine-0.4g, enadenine-0.2g, beef extract-3.3g, agar-0.25g, pH value is adjusted to 7.0, and clear water polishing is to 1000g.
4. the percentage by weight ratio of carrier auxiliary material according to claim 1 and 3 kinds of female powder of bacterial strain is: 10:0.4-1:0.4-1:0.4-1.
5. its composition by weight percent of carrier auxiliary material mixture according to claim 1 is: turfy soil 58.6%, coconut palm chaff 15%, sugared slag 15%, oxyenadenine 0.2%, enadenine 0.2%, polyacrylamide gel 2%, gum arabic powder 1%, potassium alginate 8%.
6. the biological intelligence bacterium block told of claim 1 is for the nursery of various vegetables, flowers, economic crops, to plant seedling stage and the preventive and therapeutic effect of transplanting rear soil-borne disease, particularly can there is extraordinary preventive effect to cucumber fusarium axysporum, capsicum damping off and watermelon damping off.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106431675A (en) * | 2016-10-08 | 2017-02-22 | 海南博士威农用化学有限公司 | Biological bacterial fertilizer containing bacillus subtilis and application |
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| CN112674116A (en) * | 2020-12-23 | 2021-04-20 | 宝鸡市星星协力生物有限公司 | Compound microbial agent for preventing and treating scab of crops and preparation and use methods thereof |
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| CN106431675A (en) * | 2016-10-08 | 2017-02-22 | 海南博士威农用化学有限公司 | Biological bacterial fertilizer containing bacillus subtilis and application |
| CN106588338A (en) * | 2016-12-12 | 2017-04-26 | 徐州得铸生物科技有限公司 | Microorganism organic fertilizer |
| CN108046894A (en) * | 2017-12-05 | 2018-05-18 | 上海创博生态工程有限公司 | A kind of microbial-bacterial fertilizer for improving potato ecologic planting yield and preparation method thereof |
| CN112674116A (en) * | 2020-12-23 | 2021-04-20 | 宝鸡市星星协力生物有限公司 | Compound microbial agent for preventing and treating scab of crops and preparation and use methods thereof |
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