CN106613098A - Triterygium hypoglaucum cutting propagation method - Google Patents

Triterygium hypoglaucum cutting propagation method Download PDF

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Publication number
CN106613098A
CN106613098A CN201610893365.0A CN201610893365A CN106613098A CN 106613098 A CN106613098 A CN 106613098A CN 201610893365 A CN201610893365 A CN 201610893365A CN 106613098 A CN106613098 A CN 106613098A
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China
Prior art keywords
cuttings
thunder godvine
hypoglaucum
triterygium
cutting
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CN201610893365.0A
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Chinese (zh)
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CN106613098B (en
Inventor
张金霞
简应权
吴明开
刘莉
陈雄飞
杨平飞
龙连奎
魏巍
李娟�
代剑楠
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GUIZHOU CROP VARIETIES RESOURCE INSTITUTE
Guizhou Dechangxiang Pharmaceutical Co ltd
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GUIZHOU CROPS VARIETIES RESOURCE INSTITUTE
Dechangxiang Medical Industry Co Ltd Guiyang
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings
    • A01G9/0299Handling or transporting of soil blocks or seedlings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • A01G13/02Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
    • A01G13/0231Tunnels, i.e. protective full coverings for rows of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • A01G13/02Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
    • A01G13/0256Ground coverings
    • A01G13/0268Mats or sheets, e.g. nets or fabrics
    • A01G13/0275Films
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Protection Of Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a triterygium hypoglaucum cutting propagation method. The method comprises the steps that the portions 2 cm under bevel notches of triterygium hypoglaucum cutting shoots are treated with a green plant growth regulator GGR (the concentration is 100 ppm) for 0.5 h; after cutting is conducted, the cutting shoots are covered with a layer of agricultural transparent plastic film serving as mulching film, and a layer of a small shed is built outside the mulching film; film breaking for seedling emergence is conducted in early-middle March; a shading net is erected in late April; the shading net is removed in late August. By taking a series of comprehensive cultivation measures, compared with a control group, the cutting survival rate of triterygium hypoglaucum is increased to 89.6% from 62.5%, the cutting propagation survival rate is increased by 27%, and differences are significant. Technical support is provided for gradually domesticating wild triterygium hypoglaucum into a home-planted variety. The seedling propagation problem in large-scale artificial planting of the triterygium hypoglaucum is effectively solved, and a good foundation is laid for sustainable use of resources and industrilized development.

Description

A kind of powder carries on the back thunder godvine cuttage breeding method
Technical field
The present invention relates to be related to process cuttings using green plant growth regulator, the double shed domestications of powder back of the body thunder godvine are carried out Technical field of cultivation.
Background technology
Powder back of the body thunder godvine is the perennial liana tripterygium hypoglaucum hutcs (Tripterygium of Celastraceae Thunder God Calamus Hypoglaucum (Levl.) Hutch) dry root.Abound with Guizhou, Yunnan, Guangxi, Hunan and other places.Powder back of the body thunder godvine has anti- Scorching, antitumor, immunological regulation, antifertility etc. are acted on, the effect of have clearing heat and detoxicating, promoting blood circulation and removing blood stasis, swelling and pain relieving.
At present powder back of the body Tripterygium Preparations, are widely used in treatment rheumatoid arthritis, and lupus erythematosus, essential thrombocythemia subtracts Few property purpura, more than the 50 kinds of disease such as primary glomerulopathy and malignant tumour is domestic first-selected immunodepressant.U.S., day, Fa Dengguojun research teams carry out going deep into system research to the active components of plants, and achieved with many important achievements, are allowed to into For the resources of medicinal plant that the world attractes attention.
With the development of medical and health cause, wild resource is increasingly reduced, and because powder back of the body thunder godvine Harvesting Years are long, and is needed The amount of asking increasingly increases again, and imbalance between supply and demand is increasingly highlighted, or even endangered.At present wild powder back of the body thunder godvine population quantity is limited, During introducing and planting, because outdoor cultivation is affected larger by harsh weather and drought and waterlogging, generally existing domestication is difficult, skewer Motility rate is inserted into relatively low, the problems such as increment is little.Therefore, powder back of the body thunder godvine cuttage survival rate is improved, increases the biomass of its plant, For protection powder back of the body thunder godvine germ plasm resource, it is had and its significance from the wild family's kind that is changed into.
The content of the invention
The present invention is intended to provide a kind of powder back of the body thunder godvine domestication propagation method.By being processed with green plant growth regulator Cuttage is carried out after powder back of the body thunder godvine cuttings, and one layer of agricultural transparent plastic film is covered on cuttings to do mulch film, outside mulch film again One layer of Small plastic shed is built, the miniclimate of a heat and moisture preserving is defined, so as to improve the reproductive survival rate that powder carries on the back thunder godvine, gradually Wild powder back of the body thunder godvine is tamed into kind of a kind of getting married.
The present invention is to be achieved through the following technical solutions:
Cuttage season is the annual 10-12 months.
Select loosing soil, a width of 110 × 110cm of railway carriage or compartment face length is forward opened in gentle hillside fields on the sunny side, railway carriage or compartment face surrounding begins to rehearse water Ditch, pour before cuttage one time it is permeable.
Fecula back of the body thunder godvine branch then is selected, branch upper end is cut flat, lower end is cut into smooth angle, is with concentration 0.5h at the GGR immersion thunder godvine cuttings angle bottom 2cm of 100ppm, then carries out cuttage.
After cuttage, the plastic film of layer of transparent is covered in cuttings, Small plastic shed is then built on mulch film, arched shed rack is Arch-shaped, bamboo chip makees skeleton.Underground is inserted at bamboo chip two by the width of canopy, circular arch is formed.After whole arch bars have been inserted, with completely The bright covered rearing with plastic film of wide phot.
March early and middle ten dayses, timely observation powder back of the body thunder godvine cuttings growing state, the blade top grown when 60% cuttings is to mulch film When, rupture of membranes is carried out to cuttings and is emerged.
Late April builds sunshade net and removes sunshade net to late August.Before high temperature arrives, when daytime temperature is increased to 30 When more than DEG C, sunshade net is built, to prevent burn powder from carrying on the back thunder godvine plant.After the high temperature past, when daytime temperature drops to 30 When below DEG C, sunshade net is removed in time, prevent thunder godvine branch excessive growth, be beneficial to powder back of the body thunder godvine and accumulate dry in case mistake Winter.
Technical scheme core be it is following some:
Firstth, adopt concentration and soak current-year branch 0.5h for the GGR of 100ppm, thunder godvine cuttage branch can be significantly improved The survival rate of bar.
Secondth, December carries out cutting propagation, combines covering with ground sheeting using Small plastic shed bilayer culture underglass and covers with Small plastic shed The advantage of lid, heat-insulating property and illumination condition all make moderate progress, under early spring cryogenic conditions, can be than simple covering with ground sheeting or simple Small plastic shed is covered and is colonized ahead of time, promotes powder back of the body thunder godvine to shift to an earlier date rudiment, fast-growth.Test shows:The cuttings of covering with plastic film, newly Tip growth is vigorous;Without the cuttings growth retardation of covering with plastic film, young sprout growth retardation, plant is short and small.
3rd, timely rupture of membranes is wanted to emerge, rupture of membranes is emerged late, and powder back of the body thunder godvine blade affects thereafter easily by burn Phase grows.
4th, to build in good time, remove sunshade net.Sunshade net is built for the growth of cuttage seeding plays vital work With, finding in process of the test, the cuttage seeding young sprout growth that sunshade net is built in time is vigorous, and two fraction numbers are 33.3%.And do not have Have build sunshade net cuttage seeding second branch number be 0, branches and leaves depauperation causes growth restriction, send out young sprout ability it is weaker.No The cuttage seeding of sunshade net is removed in time, and branch excessive growth is excessively prosperous, and dry-matter accumulation is few, easily by freeze injury.
Specific embodiment
With reference to embodiment, the present invention is described in further detail.Such as relating to numerical value or numeric ratio in embodiment Example, such as refers both to mass figures or mass ratio without dated.
Embodiment 1:
What the present invention was realized in:
(1) test period:On December 25th, 2015
(2) varieties of plant:Powder back of the body thunder godvine (source:Guizhou Province Leishan County Thunder God Mountain)
(3) place is planted:Guizhou Province Xiuwen County Zha Zuo towns
(4) cultural method:
Cuttings selects:Want growth selection stalwartness, the back of the body thunder godvine current-year branch of the powder without pest and disease damage.
Cuttings specification:Cuttings rugosity 8-10mm, length is 15~20cm, and each branch stays 2~3 resting buds, and upper end is cut Flat mouth, lower end cuts flat sliding angle, and clip upper end is from 1~2cm of resting bud.
Test process:Concentration is respectively 0.5h at GGR, NAA, IBA of 100ppm immersion cuttings angle bottom 2cm, Compareed with clear water immersion cuttings.Each is processed as 16 plants, is repeated 3 times, and carries out single factor test randomized block experiment.
Selection of land site preparation:Select that soil layer is deep, draining good, the sand loam rich in humus faces south hillside fields.Per mu is applied excellent Matter fertilizer 4000kg.30~40cm of deep ploughing, is layered hack, and native face will put down, want thin.By the thin leveling of plot rake, make 110 × The railway carriage or compartment face of 110cm, ditch 20cm between the face of railway carriage or compartment, and in the ranks ditch 30cm, pour behind whole complete ground one time it is permeable.
Cuttage overlay film:Powder back of the body thunder godvine cuttings angle downwards, by 2/3 insertion soil of cuttings, reserves 1~2 during cuttage Resting bud, cuttings north orientation is in 45 ° with ground, and strain spacing is 20 × 20cm.Overlay film, ridging are carried out from agriculture transparent plastic film During overlay film cuttage, it is smooth to be applied to ridge face, and epiphragma is tight, mulch film is tensed, stretched, being paved, spreads even, and the surrounding of film is respectively opened One shallow ridges, it is with wet soil that ground film compacting, pressure is tight, it is to avoid ventilative, to reach the effect for suppressing weeds and moisturizing to heat.Covering film Afterwards skeleton is done with bamboo chip, Small plastic shed, shed height 60cm, span 110cm are built on mulch film.Two neighboring arch bar spacing 80cm.To put up after skeleton and cover agricultural transparent plastic film, film surrounding soil compaction above.Early spring can be when divulging information Film base is opened in the both sides of canopy, notes controlling time and the temperature of shed of ventilation during ventilation, it is to avoid powder back of the body thunder godvine branch is received Damage to plants caused by sudden drop in temperature.
Seedling management:After the back of the body thunder godvine cuttings germination of beginning of spring powder, carry out rupture of membranes on March 15th, 2016 and emerge.Use sharp knife Rupture of membranes puts seedling, accomplishes that one puts one, with high temperature-proof burn seedlings.When noticing that seedling is put in perforate, holes size is appropriate, is with 3cm Preferably;Put with wet soil that cut envelope is real while seedling, the generation for suppressing weeds to preserve soil moisture.Never rupture of membranes too late, otherwise easy burn seedlings. Temperature in observation place condition and Small plastic shed is answered after rupture of membranes, when arch temperature of shed reaches 30 °, the film on Small plastic shed should be in time opened Leak informaton, in order to avoid burn cuttings.
Growing season management:On April 25th, 2016, mulch film and Small plastic shed are removed, build sunshade net.The thunder of sunshade net is not built Public rattan cuttings, seedling development critical constraints are sent out young sprout ability and are weakened significantly, have a strong impact on powder back of the body thunder godvine late growing stage. On August 26th, 2016, removes sunshade net, is beneficial to robust plant growth.
In 6~July, rationally impose available nitrogen, potash fertilizer to powder back of the body thunder godvine, dressing time should be arranged in growth it is vigorous before in Phase, topdress urgent, in order to avoid causing nursery stock excessive growth, reduce degree of lignification, be unfavorable for surviving the winter.
The investigation powder back of the body thunder godvine cuttings survival rate on the 7th of September in 2016, single factor test randomized block experiment variance point is carried out to it Analysis, is as a result followed successively by from high to low:
GGR (89.6%)>IBA (75%)>NAA (72.9%)>CK (62.5%).Compare with control, be using concentration Tri- kinds of hormons of the GGR of 100ppm, IBA, NAA process respectively cuttings, and cuttings survival rate is correspondingly improved, but NAA, IBA compares that difference is not notable with control, and the GGR of 100ppm immersion powder back of the body thunder godvine current-year branch 0.5h, compare with control, Cuttage survival rate significant difference.
Control GGR NAA IBA
Cuttage survival rate (%) 62.5Ab 89.6Aa 72.9Aab 75Aab
Conclusion:Using 100ppm GGR soak current-year branch 0.5h, can significantly improve thunder godvine cuttage branch into Motility rate.
Certainly, the above is the concrete application example of the present invention, and the present invention also has other embodiments, all using equivalent The technical scheme that replacement or equivalent transformation are formed, all falls within protection domain of the presently claimed invention.

Claims (5)

1. a kind of powder carries on the back thunder godvine cuttage breeding method, it is characterised in that:Processed after cuttings using green plant growth regulator, Carry out powder back of the body thunder godvine introducing and planting.
2. powder according to claim 1 carries on the back thunder godvine cuttage breeding method, it is characterised in that:The green plants growth is adjusted Section agent is GGR, and concentration is 100ppm.
3. powder according to claim 2 carries on the back thunder godvine cuttage breeding method, it is characterised in that:Select fecula back of the body Thunder God then Rattan branch, branch upper end is cut flat, and lower end is cut into smooth angle, oblique with the GGR immersions thunder godvine cuttings that concentration is 100ppm 0.5h at mouth bottom 2cm, then carries out cuttage to cuttings.
4. powder according to claim 3 carries on the back thunder godvine cuttage breeding method, it is characterised in that:After cuttage, in cuttings overlying The plastic film of lid layer of transparent, then builds Small plastic shed on mulch film, and arched shed rack is arch-shaped, and bamboo chip makees skeleton;By canopy Underground is inserted in bamboo chip two by width, forms circular arch;After whole arch bars have been inserted, with the complete bright covered rearing with plastic film of wide phot.
5. powder according to claim 4 carries on the back thunder godvine cuttage breeding method, it is characterised in that:March early and middle ten dayses, to powder thunder is carried on the back Public rattan cuttings carries out rupture of membranes and emerges.Late April, high temperature removes mulch film and Small plastic shed before arriving, and builds sunshade net, late August After the high temperature past, sunshade net is removed in time.
CN201610893365.0A 2016-10-13 2016-10-13 Cutting propagation method for Tripterygium wilfordii Active CN106613098B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107223508A (en) * 2017-05-31 2017-10-03 柳州市兰苗种植场 A kind of method of safety tree cutting propagation
CN108849301A (en) * 2018-07-26 2018-11-23 安龙县欣蔓生物科技有限责任公司 A kind of cultural method of tripterygium wilfordii

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Publication number Priority date Publication date Assignee Title
CN1994044A (en) * 2006-12-21 2007-07-11 浙江省中药研究所有限公司 Method for planting Chinese herb triperygium wilfordii
CN102106231A (en) * 2010-12-01 2011-06-29 福建省汉堂生物制药股份有限公司 Tripterygium wilfordii hook acclimatization and high-yielding method

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CN1994044A (en) * 2006-12-21 2007-07-11 浙江省中药研究所有限公司 Method for planting Chinese herb triperygium wilfordii
CN102106231A (en) * 2010-12-01 2011-06-29 福建省汉堂生物制药股份有限公司 Tripterygium wilfordii hook acclimatization and high-yielding method

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107223508A (en) * 2017-05-31 2017-10-03 柳州市兰苗种植场 A kind of method of safety tree cutting propagation
CN108849301A (en) * 2018-07-26 2018-11-23 安龙县欣蔓生物科技有限责任公司 A kind of cultural method of tripterygium wilfordii

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Address after: No. 8, Zone A, Zhazuo Pharmaceutical Park, Zhazuo Town, Xiuwen County, Guiyang City, Guizhou Province, 550299

Patentee after: Guizhou Dechangxiang Pharmaceutical Co.,Ltd.

Patentee after: GUIZHOU CROP VARIETIES RESOURCE INSTITUTE

Address before: No. 8, Zone A, Zhazuo Pharmaceutical Park, Zhazuo Town, Xiuwen County, Guiyang City, Guizhou Province, 550299

Patentee before: Guiyang Dechangxiang Pharmaceutical Co.,Ltd.

Patentee before: GUIZHOU CROP VARIETIES RESOURCE INSTITUTE