CN106536536B - 无热量甜味剂 - Google Patents
无热量甜味剂 Download PDFInfo
- Publication number
- CN106536536B CN106536536B CN201580035507.3A CN201580035507A CN106536536B CN 106536536 B CN106536536 B CN 106536536B CN 201580035507 A CN201580035507 A CN 201580035507A CN 106536536 B CN106536536 B CN 106536536B
- Authority
- CN
- China
- Prior art keywords
- rebaudioside
- product
- ppm
- sweetener
- glycosyltransferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/30—Artificial sweetening agents
- A23L27/33—Artificial sweetening agents containing sugars or derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01013—Sucrose synthase (2.4.1.13)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Seasonings (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14/269,435 US9522929B2 (en) | 2014-05-05 | 2014-05-05 | Non-caloric sweetener |
| US14/269,435 | 2014-05-05 | ||
| PCT/US2015/029163 WO2015171555A1 (en) | 2014-05-05 | 2015-05-05 | A non-caloric sweetener |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| CN106536536A CN106536536A (zh) | 2017-03-22 |
| CN106536536A8 CN106536536A8 (zh) | 2017-07-07 |
| CN106536536B true CN106536536B (zh) | 2019-12-06 |
Family
ID=54354822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580035507.3A Active CN106536536B (zh) | 2014-05-05 | 2015-05-05 | 无热量甜味剂 |
Country Status (16)
| Country | Link |
|---|---|
| US (9) | US9522929B2 (enExample) |
| EP (3) | EP3418288B1 (enExample) |
| JP (1) | JP6641357B2 (enExample) |
| KR (1) | KR102378988B1 (enExample) |
| CN (1) | CN106536536B (enExample) |
| AR (1) | AR100277A1 (enExample) |
| AU (1) | AU2015256232B2 (enExample) |
| BR (1) | BR112016025147B1 (enExample) |
| CA (1) | CA2982075A1 (enExample) |
| DK (1) | DK3140314T3 (enExample) |
| ES (1) | ES2750373T3 (enExample) |
| HU (1) | HUE045937T2 (enExample) |
| MX (1) | MX367883B (enExample) |
| NZ (1) | NZ725866A (enExample) |
| WO (1) | WO2015171555A1 (enExample) |
| ZA (1) | ZA201607592B (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9752174B2 (en) | 2013-05-28 | 2017-09-05 | Purecircle Sdn Bhd | High-purity steviol glycosides |
| US9522929B2 (en) | 2014-05-05 | 2016-12-20 | Conagen Inc. | Non-caloric sweetener |
| CN113549664B (zh) | 2014-09-19 | 2025-10-28 | 谱赛科有限责任公司 | 高纯度甜菊醇糖苷 |
| EP3197469B1 (en) | 2014-09-26 | 2023-08-09 | PureCircle USA Inc. | Stevia composition, production method and uses |
| AU2015327940B2 (en) | 2014-10-03 | 2020-04-16 | Conagen Inc. | Non-caloric sweeteners and methods for synthesizing |
| WO2016100689A1 (en) | 2014-12-17 | 2016-06-23 | Cargill, Incorporated | Steviol glycoside compounds, compositions for oral ingestion or use, and method for enhancing steviol glycoside solubility |
| EP3718417B1 (en) | 2015-04-03 | 2025-09-03 | DSM IP Assets B.V. | Steviol glycosides |
| WO2016168413A1 (en) | 2015-04-14 | 2016-10-20 | Conagen Inc. | Production of non-caloric sweeteners using engineered whole-cell catalysts |
| US10494397B2 (en) * | 2016-07-15 | 2019-12-03 | Pepsico, Inc. | Rebaudioside analogs |
| BR112019007504B1 (pt) | 2016-10-14 | 2023-05-09 | Conagen Inc | Método de preparação de uma composição de glicosídeo de esteviol |
| AU2016427130B2 (en) | 2016-10-21 | 2022-08-18 | Pepsico, Inc. | Method for preparing rebaudioside N using enzymatic method |
| JP6842800B2 (ja) * | 2016-10-21 | 2021-03-17 | ペプシコ・インク | 酵素的方法を使用してレバウディオサイドｊを調製するための方法 |
| WO2018093196A1 (ko) * | 2016-11-17 | 2018-05-24 | 서울대학교산학협력단 | 변형 류코노스톡속 균주를 이용한 스테비오사이드 배당체의 합성 방법 및 이에 의하여 제조된 신규한 스테비오사이드 배당체 |
| KR101997597B1 (ko) * | 2016-11-17 | 2019-07-08 | 서울대학교산학협력단 | 변형 류코노스톡속 균주를 이용한 스테비오사이드 배당체의 합성 방법 및 이에 의하여 제조된 신규한 스테비오사이드 배당체 |
| IL321402A (en) | 2017-02-03 | 2025-08-01 | Tate & Lyle Solutions Usa Llc | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| JP7194111B2 (ja) | 2017-03-06 | 2022-12-21 | コナゲン インコーポレイテッド | ステビオール配糖体レバウジオシドｄ４のレバウジオシドｅからの生合成製造 |
| EP3644758A4 (en) | 2017-06-30 | 2021-03-31 | Conagen Inc. | Hydrolysis of steviol glycosides by beta-glucosidase |
| CN109423486B (zh) * | 2017-08-29 | 2022-02-25 | 中国科学院分子植物科学卓越创新中心 | 新型udp-糖基转移酶及其应用 |
| CN107446874B (zh) * | 2017-09-25 | 2020-07-28 | 四川盈嘉合生科技有限公司 | 重组大肠杆菌及其在合成莱鲍迪苷d中的用途 |
| CN111868252B (zh) * | 2018-03-12 | 2025-06-03 | 科纳根公司 | 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产 |
| CN109415747B (zh) * | 2018-05-25 | 2022-05-03 | 邦泰生物工程(深圳)有限公司 | 一种酶改质甜菊糖的制备方法和制备用酶及应用 |
| MX2021001260A (es) | 2018-07-30 | 2021-04-12 | Tate & Lyle Solutions Usa Llc | Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol. |
| JP2022501309A (ja) * | 2018-09-29 | 2022-01-06 | フイルメニツヒ ソシエテ アノニムFirmenich Sa | テルペングリコシド誘導体およびその使用 |
| CN111344399B (zh) * | 2018-11-16 | 2023-03-03 | 邦泰生物工程(深圳)有限公司 | 一种udp-葡萄糖基转移酶突变体、其应用及其制备莱鲍迪苷d的方法 |
| BR112021011419A2 (pt) * | 2018-12-12 | 2021-09-08 | Conagen Inc. | Método de produção de rebaudiosídeo r6-2a e/ou r6-2b, r7-2, r6-1 e r6-4a e/ou r6-4b, rebaudiosídeo sintético e produto de consumo oral |
| CN109750072B (zh) * | 2019-01-31 | 2022-04-19 | 南京工业大学 | 一种酶法制备莱鲍迪苷e的方法 |
| JP7318989B2 (ja) * | 2019-03-29 | 2023-08-01 | コナゲン インコーポレイテッド | Ｕｄｐ－ラムノースの生合成生産 |
| CN110878288B (zh) * | 2019-12-09 | 2020-12-29 | 安徽农业大学 | 多肽、核酸及其在合成橙花叔醇糖苷上的应用 |
| CN111378630A (zh) * | 2020-04-09 | 2020-07-07 | 北京农学院 | 一种融合蛋白及其高效表达方法与应用 |
| CN114045273B (zh) * | 2021-11-15 | 2022-07-22 | 四川大学 | 糖基转移酶OsUGT91C1突变体及其应用 |
| CN118974246A (zh) * | 2021-12-24 | 2024-11-15 | 株式会社三养社 | 莱鲍迪苷的生产 |
| US12446605B2 (en) | 2022-03-23 | 2025-10-21 | Sweegen, Inc. | Sweeteners |
| CN115433702A (zh) * | 2022-08-23 | 2022-12-06 | 江南大学 | 一种利用糖基转移酶高效生物合成莱鲍迪苷d2的方法 |
| CN117070487B (zh) * | 2023-03-27 | 2024-12-24 | 浙江大学 | 茶树芦丁:尿苷二磷酸葡萄糖基转移酶基因及其应用 |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04149191A (ja) * | 1990-10-09 | 1992-05-22 | Nakano Vinegar Co Ltd | ステビオサイド糖転移化合物及びその製造方法 |
| WO2002010210A2 (en) | 2001-08-28 | 2002-02-07 | Bayer Cropscience Ag | Polypeptides for identifying herbicidally active compounds |
| US20090183270A1 (en) | 2002-10-02 | 2009-07-16 | Adams Thomas R | Transgenic plants with enhanced agronomic traits |
| US20080148432A1 (en) | 2005-12-21 | 2008-06-19 | Mark Scott Abad | Transgenic plants with enhanced agronomic traits |
| US9107436B2 (en) | 2011-02-17 | 2015-08-18 | Purecircle Sdn Bhd | Glucosylated steviol glycoside as a flavor modifier |
| US8524785B2 (en) | 2009-09-04 | 2013-09-03 | International Flavors And Fragrances Inc. | Methods for using rebaudioside C as a flavor enhancer |
| SG178376A1 (en) | 2009-09-04 | 2012-03-29 | Redpoint Bio Corp | Sweetness enhancers including rebaudioside a or d |
| EP2533651A1 (en) * | 2010-02-08 | 2012-12-19 | The Coca-Cola Company | Solubility enhanced terpene glycoside(s) |
| US9795156B2 (en) | 2011-03-17 | 2017-10-24 | E.P.C (Beijing) Plant Pharmaceutical Technology Co., Ltd | Rebaudioside B and derivatives |
| JP6262653B2 (ja) | 2011-08-08 | 2018-01-17 | エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． | ステビオール配糖体の組換え生産 |
| EP2793615A4 (en) | 2011-12-20 | 2016-07-20 | Cargill Inc | MIXTURE WITH LOW MONATIN AND REBAUDIOSIDE A CONTENT |
| FR2984743B1 (fr) | 2011-12-23 | 2014-08-22 | Oreal | Utilisation cosmetique du steviol, d'un derive glycoside du steviol, ou un de leurs isomeres pour stimuler, restaurer ou reguler le metabolisme des cellules de la peau et des semi-muqueuses. |
| FR2984742A1 (fr) | 2011-12-23 | 2013-06-28 | Oreal | Utilisation de steviol, d'un derive glycoside du steviol, ou d'un de leurs isomeres, pour prevenir, reduire et/ou traiter une alteration du teint de la peau. |
| EA201400836A8 (ru) | 2012-01-23 | 2016-03-31 | ДСМ АйПи АССЕТС Б.В. | Получение дитерпена |
| CN102559528B (zh) * | 2012-02-09 | 2013-08-21 | 南京工业大学 | 一种产甜叶菊糖基转移酶ugt76g1的基因工程菌及其应用 |
| EP2852296B1 (en) | 2012-05-22 | 2021-12-15 | PureCircle SDN BHD | Process for producing a high-purity steviol glycoside |
| US9752174B2 (en) | 2013-05-28 | 2017-09-05 | Purecircle Sdn Bhd | High-purity steviol glycosides |
| CN107659908B (zh) * | 2012-07-18 | 2021-06-22 | 华为技术有限公司 | 一种数据连接管理的方法、装置及系统 |
| CN103570723B (zh) | 2012-07-27 | 2016-07-13 | 广西梧州制药（集团）股份有限公司 | 吡唑并嘧啶衍生物及其制备方法和在药物制备中的用途 |
| CN102766667B (zh) * | 2012-08-14 | 2014-06-11 | 成都南诺格生物科技有限责任公司 | 甜菊苷转化为莱鲍迪苷e的方法 |
| KR20150115002A (ko) * | 2013-02-06 | 2015-10-13 | 에볼바 에스아 | 레바우디오시드 ｄ 및 레바우디오시드 ｍ의 개선된 생산 방법 |
| KR101404728B1 (ko) | 2013-02-28 | 2014-06-09 | 씨제이제일제당 (주) | 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법 |
| CN103397064B (zh) | 2013-08-14 | 2015-04-15 | 苏州汉酶生物技术有限公司 | 一种酶法制备瑞鲍迪甙m的方法 |
| JP6530760B2 (ja) | 2013-11-01 | 2019-06-12 | コナゲン インコーポレーテッド | ステビオール配糖体の組換え製造 |
| US9522929B2 (en) | 2014-05-05 | 2016-12-20 | Conagen Inc. | Non-caloric sweetener |
| CN104232496B (zh) | 2014-09-18 | 2017-06-06 | 广州康琳奈生物科技有限公司 | 一种重组毕赤酵母工程菌及其在合成莱鲍迪苷a中的应用 |
| AU2015327940B2 (en) | 2014-10-03 | 2020-04-16 | Conagen Inc. | Non-caloric sweeteners and methods for synthesizing |
| WO2016168413A1 (en) | 2015-04-14 | 2016-10-20 | Conagen Inc. | Production of non-caloric sweeteners using engineered whole-cell catalysts |
| WO2017122227A1 (en) | 2016-01-13 | 2017-07-20 | Shri Amm Murugappa Chettiar Research Centre (Mcrc) | Mrsa and vrsa resistant textile materials |
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2014
- 2014-05-05 US US14/269,435 patent/US9522929B2/en active Active
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2015
- 2015-05-05 DK DK15789509.5T patent/DK3140314T3/da active
- 2015-05-05 EP EP18187127.8A patent/EP3418288B1/en active Active
- 2015-05-05 ES ES15789509T patent/ES2750373T3/es active Active
- 2015-05-05 WO PCT/US2015/029163 patent/WO2015171555A1/en not_active Ceased
- 2015-05-05 MX MX2016014506A patent/MX367883B/es active IP Right Grant
- 2015-05-05 HU HUE15789509A patent/HUE045937T2/hu unknown
- 2015-05-05 AU AU2015256232A patent/AU2015256232B2/en active Active
- 2015-05-05 CA CA2982075A patent/CA2982075A1/en active Pending
- 2015-05-05 NZ NZ725866A patent/NZ725866A/en unknown
- 2015-05-05 JP JP2017511156A patent/JP6641357B2/ja active Active
- 2015-05-05 CN CN201580035507.3A patent/CN106536536B/zh active Active
- 2015-05-05 BR BR112016025147-4A patent/BR112016025147B1/pt active IP Right Grant
- 2015-05-05 EP EP22151239.5A patent/EP4023660A1/en active Pending
- 2015-05-05 AR ARP150101348A patent/AR100277A1/es unknown
- 2015-05-05 KR KR1020167033558A patent/KR102378988B1/ko active Active
- 2015-05-05 EP EP15789509.5A patent/EP3140314B1/en active Active
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2016
- 2016-02-05 US US15/016,750 patent/US10138263B2/en active Active
- 2016-02-05 US US15/016,725 patent/US9765104B2/en active Active
- 2016-02-05 US US15/016,589 patent/US9527880B2/en active Active
- 2016-11-03 ZA ZA2016/07592A patent/ZA201607592B/en unknown
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2017
- 2017-09-12 US US15/701,613 patent/US10654881B2/en active Active
- 2017-09-12 US US15/701,599 patent/US10597418B2/en active Active
- 2017-09-29 US US15/719,669 patent/US9988414B2/en active Active
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2018
- 2018-04-30 US US15/966,224 patent/US10450339B2/en active Active
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2020
- 2020-04-10 US US16/846,116 patent/US10941174B2/en active Active
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| HK1237787B (zh) | 无热量甜味剂及合成方法 | |
| HK1236753A1 (en) | Non-caloric sweeteners and methods for synthesizing | |
| HK1237787A1 (en) | Non-caloric sweeteners and methods for synthesizing | |
| HK1237822A1 (en) | Non-caloric sweeteners and methods for synthesizing |
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