BR112016025147B1 - Rebaudiosídeo sintético, seu método de síntese,produto de consumo por via oral e composição adoçante - Google Patents

Rebaudiosídeo sintético, seu método de síntese,produto de consumo por via oral e composição adoçante Download PDF

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Publication number
BR112016025147B1
BR112016025147B1 BR112016025147-4A BR112016025147A BR112016025147B1 BR 112016025147 B1 BR112016025147 B1 BR 112016025147B1 BR 112016025147 A BR112016025147 A BR 112016025147A BR 112016025147 B1 BR112016025147 B1 BR 112016025147B1
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BR
Brazil
Prior art keywords
rebaudioside
udp
formula
sucrose synthase
glycosyltransferase
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BR112016025147-4A
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English (en)
Portuguese (pt)
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BR112016025147A2 (pt
Inventor
Guohong Mao
Venkata Sai Prakash Chaturvedula
Oliver Yu
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Conagen Inc.
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Application filed by Conagen Inc. filed Critical Conagen Inc.
Publication of BR112016025147A2 publication Critical patent/BR112016025147A2/pt
Publication of BR112016025147B1 publication Critical patent/BR112016025147B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
BR112016025147-4A 2014-05-05 2015-05-05 Rebaudiosídeo sintético, seu método de síntese,produto de consumo por via oral e composição adoçante BR112016025147B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/269,435 2014-05-05
US14/269,435 US9522929B2 (en) 2014-05-05 2014-05-05 Non-caloric sweetener
PCT/US2015/029163 WO2015171555A1 (en) 2014-05-05 2015-05-05 A non-caloric sweetener

Publications (2)

Publication Number Publication Date
BR112016025147A2 BR112016025147A2 (pt) 2017-12-12
BR112016025147B1 true BR112016025147B1 (pt) 2021-11-03

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BR112016025147-4A BR112016025147B1 (pt) 2014-05-05 2015-05-05 Rebaudiosídeo sintético, seu método de síntese,produto de consumo por via oral e composição adoçante

Country Status (16)

Country Link
US (9) US9522929B2 (enExample)
EP (3) EP4023660A1 (enExample)
JP (1) JP6641357B2 (enExample)
KR (1) KR102378988B1 (enExample)
CN (1) CN106536536B (enExample)
AR (1) AR100277A1 (enExample)
AU (1) AU2015256232B2 (enExample)
BR (1) BR112016025147B1 (enExample)
CA (1) CA2982075A1 (enExample)
DK (1) DK3140314T3 (enExample)
ES (1) ES2750373T3 (enExample)
HU (1) HUE045937T2 (enExample)
MX (1) MX367883B (enExample)
NZ (1) NZ725866A (enExample)
WO (1) WO2015171555A1 (enExample)
ZA (1) ZA201607592B (enExample)

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AU2015364481B2 (en) 2014-12-17 2020-05-14 Cargill, Incorporated Steviol glycoside compounds, compositions for oral ingestion or use, and method for enhancing steviol glycoside solubility
EP3718417B1 (en) 2015-04-03 2025-09-03 DSM IP Assets B.V. Steviol glycosides
EP3283614B1 (en) 2015-04-14 2020-07-08 Conagen Inc. Production of non-caloric sweeteners using engineered whole-cell catalysts
US10494397B2 (en) * 2016-07-15 2019-12-03 Pepsico, Inc. Rebaudioside analogs
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CN111868252B (zh) * 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产
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CN111378630A (zh) * 2020-04-09 2020-07-07 北京农学院 一种融合蛋白及其高效表达方法与应用
CN114045273B (zh) * 2021-11-15 2022-07-22 四川大学 糖基转移酶OsUGT91C1突变体及其应用
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BR112017006139B1 (pt) 2014-10-03 2022-04-05 Conagen Inc Método para a sintetização de rebaudiosídeo m ou rebaudiosídeo d e rebaudiosídeo m
EP3283614B1 (en) 2015-04-14 2020-07-08 Conagen Inc. Production of non-caloric sweeteners using engineered whole-cell catalysts
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Publication number Publication date
EP3140314A4 (en) 2017-10-18
US9765104B2 (en) 2017-09-19
ZA201607592B (en) 2019-02-27
AU2015256232A1 (en) 2016-11-24
EP3140314B1 (en) 2019-07-31
MX367883B (es) 2019-09-10
CN106536536A8 (zh) 2017-07-07
WO2015171555A1 (en) 2015-11-12
US20200361978A1 (en) 2020-11-19
CN106536536A (zh) 2017-03-22
KR20160147994A (ko) 2016-12-23
CA2982075A1 (en) 2015-11-12
US20160208225A1 (en) 2016-07-21
MX2016014506A (es) 2017-02-23
US10450339B2 (en) 2019-10-22
US10654881B2 (en) 2020-05-19
BR112016025147A2 (pt) 2017-12-12
KR102378988B1 (ko) 2022-03-28
US10597418B2 (en) 2020-03-24
US20180244709A1 (en) 2018-08-30
AR100277A1 (es) 2016-09-21
JP6641357B2 (ja) 2020-02-05
US9527880B2 (en) 2016-12-27
EP4023660A1 (en) 2022-07-06
US20160207954A1 (en) 2016-07-21
NZ725866A (en) 2022-11-25
US10941174B2 (en) 2021-03-09
EP3418288B1 (en) 2022-01-19
JP2017519812A (ja) 2017-07-20
CN106536536B (zh) 2019-12-06
DK3140314T3 (da) 2019-10-21
US20150315623A1 (en) 2015-11-05
EP3418288A1 (en) 2018-12-26
HUE045937T2 (hu) 2020-01-28
EP3140314A1 (en) 2017-03-15
ES2750373T3 (es) 2020-03-25
AU2015256232B2 (en) 2019-09-26
US20180051049A1 (en) 2018-02-22
US20160208303A1 (en) 2016-07-21
US9988414B2 (en) 2018-06-05
US20180009835A1 (en) 2018-01-11
US20180037600A1 (en) 2018-02-08
US9522929B2 (en) 2016-12-20
US10138263B2 (en) 2018-11-27

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