CN106278403A - A kind of agriculture microorganism formulation for soil in protected field conditioning and preparation method thereof - Google Patents
A kind of agriculture microorganism formulation for soil in protected field conditioning and preparation method thereof Download PDFInfo
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- CN106278403A CN106278403A CN201610658001.4A CN201610658001A CN106278403A CN 106278403 A CN106278403 A CN 106278403A CN 201610658001 A CN201610658001 A CN 201610658001A CN 106278403 A CN106278403 A CN 106278403A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of agriculture microorganism formulation for soil in protected field conditioning and preparation method thereof.This agricultural microorganism formulation comprises powder of straw, humic acids, alginic acid, moisture, zymocyte, formula bacterium, and the proportioning by weight each component is respectively 80 90:2 4:2 4:2 4:0.5 1:0.5 1.First straw is broken into powder of straw, size is more than 100 mesh, it is by weight: the zymocyte of 0.5 1:2 4:2 4, alginic acid, water are sprayed in the powder of straw by weight 80 90 uniformly, it is uniformly mixed, fermenting after sealing, the persistent period is 10 16 days, and fermentation Stress control is at 0.3 0.5MPa, temperature is 28 35 DEG C, pH 67；After having fermented, add humic acids and formula bacterium by weight 2 4:0.5 1, be mixed into final products.The present invention utilizes straw for fermentation raw material, utilizes microorganism to ferment, and utilizes alginic acid and humic acids as adjuvant simultaneously, it is thus achieved that agriculture microorganism formulation, in terms of soil conditioning, obtain good effect, turn waste into wealth, protect environment.
Description
Technical field
The present invention relates to biological husbantry technical field, particularly relate to a kind of agriculture microorganism system for soil in protected field conditioning
Agent and preparation method thereof.
Background technology
Soil is the basic of our human survival.Along with the progress of society, science and technology is the most flourishing, various predation formulas
Development so that our this sheet soil of life has been scarred the most.So we have to take corresponding measure, carry out
Soil remediation, for our arable land, the crop yield appearing as us of chemical fertilizer was once made " distinctions won on the battlefield ", but in recent years
In order to devote exclusive attention to output, blindly, excess chemical fertilizer put into, have ignored the use of fertilizer, cause adding input cost, but do not have
Make yield increase, cause serious infringement to soil on the contrary, occur including organic on the low side, soil acidification, secondary salinization,
Soil compactions etc. are at interior soil degradation phenomenon, the fertility wretched insufficiency of soil.Especially soil in protected field environment, because facility steams
Sending out amount big, do not have again effective precipitation, the garden crop that economic worth of in addition planting in facility is higher, fertilizer application amount is bigger,
Therefore in facility, soil environment is the most severe.So, study soil amelioration techniques, reasonably soil in protected field improved,
It it is our problem demanding prompt solution.
Straw is the general name of ripe crops stem and leaf (fringe) part.It is often referred to Semen Tritici aestivi, Oryza sativa L., Semen Maydis, potato class, Brassica campestris L, cotton
Flower, Caulis Sacchari sinensis and other crops (usually coarse grain) remainder after results seed.The photosynthetic product of crops has
More than half is present in straw, straw rich in nitrogen, phosphorus, potassium, calcium, magnesium and organic matter etc., be one have multiduty can be again
Raw living resources.But, current straw is often taken as a kind of waste material, and is burned by people, so causes environment
Great pollution, meets again the rule of agricultural sustainable development simultaneously.For being raw-material product with straw, at present in state
Interior also in the starting stage, the occupation mode for straw is also that technology content is the highest, with other materials with simple also Tian Weizhu
The product of integrated application is deficient.
Alginic acid and humic acids show to improve soil physico-chemical property in substantial amounts of research, keep soil from packing together, and increase
Add the soil organism.Regulation soil pH, but also rarely have report about mixing the research playing improved effect with straw.
Microorganism has improvement soil, discharges plant active factors, promotes plant root growth, strengthens crop resistance,
Improve the yield and quality of crop and the advantage such as free from environmental pollution, be widely used in agriculture field in recent years.
Summary of the invention
It is an object of the invention to provide a kind of agriculture microorganism formulation for soil in protected field conditioning and preparation method thereof.Profit
Be fermentation raw material with straw, utilize microorganism to ferment, utilize alginic acid and humic acids as adjuvant simultaneously, it is thus achieved that be used for
The agriculture microorganism formulation of soil in protected field conditioning, obtains good effect in terms of soil conditioning, turns waste into wealth, protect environment,
Meet the requirement of this reusable edible of modern agriculture simultaneously, there is boundless prospect.
A kind of agriculture microorganism formulation for soil in protected field conditioning that the present invention provides.Described agricultural microorganism formulation bag
Containing powder of straw, humic acids, alginic acid, moisture, zymocyte, formula bacterium, the proportioning by weight each component is respectively 80-90:2-
4:2-4:2-4:0.5-1:0.5-1.
Preferably, described zymocyte is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 20-30:30-
40:20-35.
Preferably, described formula bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, brown ball
Shape nitrogen-fixing bacteria, its mass ratio is 30:15:12:12.
The present invention additionally provides a kind of agriculture microorganism formulation preparation method for soil in protected field conditioning, first by straw
Being broken into powder of straw, then size is more than 100 mesh, is the most by weight: the zymocyte of 0.5-1:2-4:2-4, alginic acid, water
Being sprayed at uniformly in the powder of straw by weight 80-90, be uniformly mixed, ferment after sealing, the persistent period is 10-
16 days, fermentation Stress control was 28-35 DEG C in 0.3-0.5MPa, temperature, pH 6-7；After having fermented, by weight 2-4:
0.5-1 adds humic acids and formula bacterium, is mixed into final products.
Preferably, described zymocyte is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 20-30:30-
40:20-35, wherein, described bacillus subtilis: carry out bacillus subtilis cultivation under aerobic environment, is 3-for inoculum concentration
5%, cultivate 12-16 hour, pH value 7.0-7.2, temperature 35 DEG C；Culture fluid is designated as peptone 1.0%, yeast powder by weight
0.5%, starch 3%, NaCl 1.0%, the MnSO of 25% concentration40.2%, Na2HPO40.2%, MgSO40.1%, surplus
For 1000ml distilled water.
Preferably, described formula bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, brown ball
Shape nitrogen-fixing bacteria, its mass ratio is 30:15:12:12.
Preferably, described gel-shaped bacillus: carry out gel-shaped bacillus cultivation under aerobic environment, for inoculum concentration
For 3-5%, cultivate 35-45 hour, pH value 7.5-8.0, temperature 37 DEG C；Culture fluid is designated as molasses 3%, bean cake powder by weight
0.6%, MgSO4·7H2O 0.15%, K2HPO40.05%, CaCO30.2%, surplus is 1000ml distilled water.
Preferably, described bacillus megaterium: carry out bacillus megaterium cultivation under aerobic environment, is 3-for inoculum concentration
5%, cultivate 30-36 hour, pH value 7.0-7.2, temperature 30 DEG C；Culture fluid is designated as starch 2% by weight, bean cake powder 4.6%,
KH2PO40.2%, Na2HPO40.05%, MgSO4·7H2O 0.05%, MnSO40.05%, surplus is 1000ml distilled water.
Preferably, described aztobacter sp: carry out aztobacter sp cultivation under aerobic environment, for inoculum concentration
For 3-5%, cultivate 24-36 hour, pH value 7.0-7.2, temperature 28 DEG C；Culture fluid is designated as molasses 2%, KH by weight2PO4
0.05%, MgSO4·7H2O 0.05%, CaCO30.05%, NaCl 0.02%, surplus is 1000ml distilled water.
Beneficial effect: the present invention utilizes straw for fermentation raw material, can increase the soil organism, improves Soil structure, makes soil
Earth loosens, and porosity increases, and capacity alleviates, and promotes microbial activity and the growth of crop root.It is simultaneously introduced alginic acid, Sargassum
Acid can promote the formation of soil aggregate, improves soil internal void space, coordinates solid, liquid, gas proportions in soil, extensive
Multiple due to soil, over-burden and chemical contamination and the lac balance that loses, increases geobiont vigor, promotes quick-acting supporting
The release divided, beneficially root growth, improves the resistance of crop.
Dissolving phosphor and dissolving potassium nitrogen-fixing microorganism flora can activating soil, improve the crop absorption to nutrient.
The adjuvant humic acid added can improve soil aggregate, regulates soil pH value, and chelated microelement is easy to plant
Thing absorbs.
Detailed description of the invention
Technical scheme and the technique effect reached for making to present invention solves the technical problem that, using are clearer, below
The present invention is described in further detail in conjunction with the embodiments.It is understood that specific embodiment described herein is only
For explaining the present invention, rather than limitation of the invention.It also should be noted that, for the ease of describe, in illustrate only
Part related to the present invention rather than full content.
Embodiment 1
Formula: described agricultural microorganism formulation comprises powder of straw, humic acids, alginic acid, moisture, zymocyte, formula bacterium, presses
The proportioning of each component of weight ratio is respectively 80:2:3:2:0.5:1.Described zymocyte is yeast, bacillus bifidus and addicted to yogurt
Bacillus, its mass ratio is 20:35:28.Described formula bacterium includes bacillus subtilis, gel-shaped bacillus, huge spore bar
Bacterium, aztobacter sp, its mass ratio is 30:15:12:12.
Preparation method: first straw is broken into powder of straw, then size is more than 100 mesh, is the most by weight: 0.5:
The zymocyte of 3:2, alginic acid, water are sprayed in the powder of straw by weight 80 uniformly, are uniformly mixed, carry out after sealing
Fermentation, the persistent period is 12 days, and fermentation Stress control is 28-35 DEG C in 0.3-0.5MPa, temperature, pH 6-7；After having fermented,
Add humic acids and formula bacterium by weight 2:1, be mixed into final products.
Described zymocyte is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 20:35:28.Described formula
Bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, aztobacter sp, and its mass ratio is 30:
15:12:12.
Described bacillus subtilis: carry out bacillus subtilis cultivation under aerobic environment, is 5% for inoculum concentration, cultivates 12
Hour, pH value 7.0-7.2, temperature 35 DEG C；Culture fluid is designated as peptone 1.0% by weight, yeast powder 0.5%, starch 3%,
NaCl 1.0%, the MnSO of 25% concentration40.2%, Na2HPO40.2%, MgSO40.1%, surplus is 1000ml distilled water.
Described gel-shaped bacillus: carry out gel-shaped bacillus cultivation under aerobic environment, is 3% for inoculum concentration,
Cultivate 40 hours, pH value 7.5-8.0, temperature 37 DEG C；Culture fluid is designated as molasses 3%, bean cake powder 0.6%, MgSO4 by weight
7H2O 0.15%, K2HPO40.05%, CaCO30.2%, surplus is 1000ml distilled water.
Described bacillus megaterium: carry out bacillus megaterium cultivation under aerobic environment, is 3% for inoculum concentration, cultivates
34 hours, pH value 7.0-7.2, temperature 30 DEG C；Culture fluid is designated as starch 2%, bean cake powder 4.6%, KH by weight2PO40.2%,
Na2HPO40.05%, MgSO4·7H2O 0.05%, MnSO40.05%, surplus is 1000ml distilled water.
Described aztobacter sp: carry out aztobacter sp cultivation under aerobic environment, is 5% for inoculum concentration,
Cultivate 24 hours, pH value 7.0-7.2, temperature 28 DEG C；Culture fluid is designated as molasses 2%, KH by weight2PO40.05%, MgSO4·
7H2O 0.05%, CaCO30.05%, NaCl 0.02%, surplus is 1000ml distilled water.
Use object: plant the salt-soda soil of Fructus Lycopersici esculenti
The use time: before field planting 2 weeks
Using method: 15 kilograms every mu
Effect: compared with the control, hence it is evident that improve the content of organic matter of soil, available nitrogen, available potassium, the containing of rapid available phosphorus
Amount, soil urease liveness, reduce the pH value of soil.Tomato Root System is flourishing, and yield adds 15.7%, and soluble sugar content is
1.92 times of comparison.
Embodiment 2
Formula: described agricultural microorganism formulation comprises powder of straw, humic acids, alginic acid, moisture, zymocyte, formula bacterium, presses
The proportioning of each component of weight ratio is respectively 90:4:2:3:1:0.5.Described zymocyte is yeast, bacillus bifidus and addicted to yogurt
Bacillus, its mass ratio is 25:30:20.Described formula bacterium includes bacillus subtilis, gel-shaped bacillus, huge spore bar
Bacterium, aztobacter sp, its mass ratio is 30:15:12:12.
Preparation method: first straw is broken into powder of straw, then size is more than 100 mesh, is the most by weight: 1:2:
The zymocyte of 3, alginic acid, water are sprayed in the powder of straw by weight 90 uniformly, are uniformly mixed, and carry out sending out after sealing
Ferment, the persistent period is 14 days, and fermentation Stress control is 28-35 DEG C in 0.3-0.5MPa, temperature, pH 6-7；After having fermented, press
Weight ratio 4:0.5 adds humic acids and formula bacterium, is mixed into final products.
Described zymocyte is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 25:30:20.
Described formula bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, the spherical fixed nitrogen of brown
Bacterium, its mass ratio is 30:15:12:12.
Described bacillus subtilis: carry out bacillus subtilis cultivation under aerobic environment, is 3% for inoculum concentration, cultivates 16
Hour, pH value 7.0-7.2, temperature 35 DEG C；Culture fluid is designated as peptone 1.0% by weight, yeast powder 0.5%, starch 3%,
NaCl 1.0%, the MnSO of 25% concentration40.2%, Na2HPO40.2%, MgSO40.1%, surplus is 1000ml distilled water.
Described gel-shaped bacillus: carry out gel-shaped bacillus cultivation under aerobic environment, is 4% for inoculum concentration,
Cultivate 40 hours, pH value 7.5-8.0, temperature 37 DEG C；Culture fluid is designated as molasses 3%, bean cake powder 0.6%, MgSO by weight4·
7H2O 0.15%, K2HPO40.05%, CaCO30.2%, surplus is 1000ml distilled water.
Described bacillus megaterium: carry out bacillus megaterium cultivation under aerobic environment, is 3% for inoculum concentration, cultivates
34 hours, pH value 7.0-7.2, temperature 30 DEG C；Culture fluid is designated as starch 2%, bean cake powder 4.6%, KH by weight2PO40.2%,
Na2HPO40.05%, MgSO4·7H2O 0.05%, MnSO40.05%, surplus is 1000ml distilled water.
Described aztobacter sp: carry out aztobacter sp cultivation under aerobic environment, is 5% for inoculum concentration,
Cultivate 28 hours, pH value 7.0-7.2, temperature 28 DEG C；Culture fluid is designated as molasses 2%, KH by weight2PO40.05%, MgSO4·
7H2O 0.05%, CaCO30.05%, NaCl 0.02%, surplus is 1000ml distilled water.
Use object: plant the acid ground of peach tree
The use time: use as base fertilizer
Using method: 600 grams/
Effect: compared with the control, the general red situation of soil is obviously improved, and the soil organism increases, and improving activity of root system improves.Blade
Dark green glossy, fruit soluble solids content improves, and fruit sugar-acid ratio improves, and flavor substance content increases.Single fruit is brought up again
Height, fruiting period is ahead of time.
Embodiment 3
Formula: described agricultural microorganism formulation comprises powder of straw, humic acids, alginic acid, moisture, zymocyte, formula bacterium, presses
The proportioning of each component of weight ratio is respectively 85:3:4:4:1:1.Described zymocyte is yeast, bacillus bifidus and addicted to yogurt bar
Bacterium, its mass ratio is 30:40:35.Described formula bacterium include bacillus subtilis, gel-shaped bacillus, bacillus megaterium,
Aztobacter sp, its mass ratio is 30:15:12:12.
Preparation method: first straw is broken into powder of straw, then size is more than 100 mesh, is the most by weight: 1:4:
The zymocyte of 4, alginic acid, water are sprayed in the powder of straw by weight 85 uniformly, are uniformly mixed, and carry out sending out after sealing
Ferment, the persistent period is 16 days, and fermentation Stress control is 28-35 DEG C in 0.3-0.5MPa, temperature, pH 6-7；After having fermented, press
Weight ratio 3:1 adds humic acids and formula bacterium, is mixed into final products.
Described zymocyte is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 30:40:35.
Described formula bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, the spherical fixed nitrogen of brown
Bacterium, its mass ratio is 30:15:12:12.
Described bacillus subtilis: carry out bacillus subtilis cultivation under aerobic environment, is 5% for inoculum concentration, cultivates 16
Hour, pH value 7.0-7.2, temperature 35 DEG C；Culture fluid is designated as peptone 1.0% by weight, yeast powder 0.5%, starch 3%,
NaCl 1.0%, the MnSO of 25% concentration40.2%, Na2HPO40.2%, MgSO40.1%, surplus is 1000ml distilled water.
Described gel-shaped bacillus: carry out gel-shaped bacillus cultivation under aerobic environment, is 3% for inoculum concentration,
Cultivate 35 hours, pH value 7.5-8.0, temperature 37 DEG C；Culture fluid is designated as molasses 3%, bean cake powder 0.6%, MgSO by weight4·
7H2O 0.15%, K2HPO40.05%, CaCO30.2%, surplus is 1000ml distilled water.
Described bacillus megaterium: carry out bacillus megaterium cultivation under aerobic environment, is 4% for inoculum concentration, cultivates
36 hours, pH value 7.0-7.2, temperature 30 DEG C；Culture fluid is designated as starch 2%, bean cake powder 4.6%, KH by weight2PO40.2%,
Na2HPO40.05%, MgSO4·7H2O 0.05%, MnSO40.05%, surplus is 1000ml distilled water.
Described aztobacter sp: carry out aztobacter sp cultivation under aerobic environment, is 3% for inoculum concentration,
Cultivate 36 hours, pH value 7.0-7.2, temperature 28 DEG C；Culture fluid is designated as molasses 2%, KH by weight2PO40.05%, MgSO4·
7H2O 0.05%, CaCO30.05%, NaCl 0.02%, surplus is 1000ml distilled water.
Use object: plant the acid ground of Fructus Vitis viniferae
The use time: after gathering in season on Fructus Vitis viniferae 1 week
Using method: 25 kilograms every mu
Effect: compared with the control, soil pH value improves 2 units；Grape root system is flourishing, and blade thickness is green, without yellow senilism
Phenomenon, fruit ear length and uniform, swollen fruit is normal, without big fruitlet, uniform coloring.
Embodiment 4
Formula: described agricultural microorganism formulation comprises powder of straw, humic acids, alginic acid, moisture, zymocyte, formula bacterium, presses
The proportioning of each component of weight ratio is respectively 85:4:4:3:1:1.Described zymocyte is yeast, bacillus bifidus and addicted to yogurt bar
Bacterium, its mass ratio is 25:40:20.Described formula bacterium include bacillus subtilis, gel-shaped bacillus, bacillus megaterium,
Aztobacter sp, its mass ratio is 30:15:12:12.
Preparation method: first straw is broken into powder of straw, then size is more than 100 mesh, is the most by weight: 1:4:
The zymocyte of 3, alginic acid, water are sprayed in the powder of straw by weight 85 uniformly, are uniformly mixed, and carry out sending out after sealing
Ferment, the persistent period is 15 days, and fermentation Stress control is 28-35 DEG C in 0.3-0.5MPa, temperature, pH 6-7；After having fermented, press
Weight ratio 4:1 adds humic acids and formula bacterium, is mixed into final products.
Described zymocyte is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 25:40:20.
Described formula bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, the spherical fixed nitrogen of brown
Bacterium, its mass ratio is 30:15:12:12.
Described bacillus subtilis: carry out bacillus subtilis cultivation under aerobic environment, is 4% for inoculum concentration, cultivates 15
Hour, pH value 7.0-7.2, temperature 35 DEG C；Culture fluid is designated as peptone 1.0% by weight, yeast powder 0.5%, starch 3%,
NaCl 1.0%, the MnSO of 25% concentration40.2%, Na2HPO40.2%, MgSO40.1%, surplus is 1000ml distilled water.
Described gel-shaped bacillus: carry out gel-shaped bacillus cultivation under aerobic environment, is 5% for inoculum concentration,
Cultivate 35 hours, pH value 7.5-8.0, temperature 37 DEG C；Culture fluid is designated as molasses 3%, bean cake powder 0.6%, MgSO by weight4·
7H2O 0.15%, K2HPO40.05%, CaCO30.2%, surplus is 1000ml distilled water.
Described bacillus megaterium: carry out bacillus megaterium cultivation under aerobic environment, is 3% for inoculum concentration, cultivates
30 hours, pH value 7.0-7.2, temperature 30 DEG C；Culture fluid is designated as starch 2%, bean cake powder 4.6%, KH by weight2PO40.2%,
Na2HPO40.05%, MgSO4·7H2O 0.05%, MnSO40.05%, surplus is 1000ml distilled water.
Described aztobacter sp: carry out aztobacter sp cultivation under aerobic environment, is 5% for inoculum concentration,
Cultivate 32 hours, pH value 7.0-7.2, temperature 28 DEG C；Culture fluid is designated as molasses 2%, KH by weight2PO40.05%, MgSO4·
7H2O 0.05%, CaCO30.05%, NaCl 0.02%, surplus is 1000ml distilled water.
Use object: plant the acid ground of Citrus
The use time: the first tenday period of a month in late September to October
Using method: 15 kilograms every mu
Effect: compared with the control, soil pH value improves, and crumb structure is improved, and Citrus grow the new root of a large amount of white, nothing
Physiological Huang leaf, young sprout is the most healthy and the strongest, and overall tree vigo(u)r has strengthened.
Embodiment 5
Formula: described agricultural microorganism formulation comprises powder of straw, humic acids, alginic acid, moisture, zymocyte, formula bacterium, presses
The proportioning of each component of weight ratio is respectively 90:3:4:2:0.5:1.Described zymocyte is yeast, bacillus bifidus and addicted to yogurt
Bacillus, its mass ratio is 20:30:35.Described formula bacterium includes bacillus subtilis, gel-shaped bacillus, huge spore bar
Bacterium, aztobacter sp, its mass ratio is 30:15:12:12.
Preparation method: first straw is broken into powder of straw, then size is more than 100 mesh, is the most by weight: 0.5:
The zymocyte of 4:2, alginic acid, water are sprayed in the powder of straw by weight 90 uniformly, are uniformly mixed, carry out after sealing
Fermentation, the persistent period is 10 days, and fermentation Stress control is 28-35 DEG C in 0.3-0.5MPa, temperature, pH 6-7；After having fermented,
Add humic acids and formula bacterium by weight 3:1, be mixed into final products.
Described zymocyte is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 20:30:35.
Described formula bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, the spherical fixed nitrogen of brown
Bacterium, its mass ratio is 30:15:12:12.
Described bacillus subtilis: carry out bacillus subtilis cultivation under aerobic environment, is 3% for inoculum concentration, cultivates 14
Hour, pH value 7.0-7.2, temperature 35 DEG C；Culture fluid is designated as peptone 1.0% by weight, yeast powder 0.5%, starch 3%,
NaCl 1.0%, the MnSO of 25% concentration40.2%, Na2HPO40.2%, MgSO40.1%, surplus is 1000ml distilled water.
Described gel-shaped bacillus: carry out gel-shaped bacillus cultivation under aerobic environment, is 4% for inoculum concentration,
Cultivate 45 hours, pH value 7.5-8.0, temperature 37 DEG C；Culture fluid is designated as molasses 3%, bean cake powder 0.6%, MgSO by weight4·
7H2O 0.15%, K2HPO40.05%, CaCO30.2%, surplus is 1000ml distilled water.
Described bacillus megaterium: carry out bacillus megaterium cultivation under aerobic environment, is 5% for inoculum concentration, cultivates
32 hours, pH value 7.0-7.2, temperature 30 DEG C；Culture fluid is designated as starch 2%, bean cake powder 4.6%, KH by weight2PO40.2%,
Na2HPO40.05%, MgSO4·7H2O 0.05%, MnSO40.05%, surplus is 1000ml distilled water.
Described aztobacter sp: carry out aztobacter sp cultivation under aerobic environment, is 4% for inoculum concentration,
Cultivate 24 hours, pH value 7.0-7.2, temperature 28 DEG C；Culture fluid is designated as molasses 2%, KH by weight2PO40.05%, MgSO4·
7H2O 0.05%, CaCO30.05%, NaCl 0.02%, surplus is 1000ml distilled water.
Use object: the continuous cropping Fructus Melo soil of 3 years
The use time: after field planting 2 weeks
Using method: 10 kilograms every mu
Effect: compared with the control, growing way is strong, and disease resistance is strong, and percentage of fertile fruit is high；Dark green leaf color, stem is slightly thick compared with matched group；Bloom
Phase is in advance；Process group fruit marketability is good, and bright in color, fruit type size are uniform, and pol improves.Rate of growth is 21.8%.
Claims (10)
1. the agriculture microorganism formulation for soil in protected field conditioning, it is characterised in that described agricultural microorganism formulation comprises
Powder of straw, humic acids, alginic acid, moisture, zymocyte, formula bacterium, be respectively 80-90:2-4 by weight the proportioning of each component:
2-4:2-4:0.5-1:0.5-1.
Agriculture microorganism formulation for soil in protected field conditioning the most according to claim 1, it is characterised in that described fermentation
Bacterium is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 20-30:30-40:20-35.
Agriculture microorganism formulation for soil in protected field conditioning the most according to claim 1, it is characterised in that described formula
Bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, aztobacter sp, and its mass ratio is 30:
15:12:12.
4. the agriculture microorganism formulation preparation method for soil in protected field conditioning, it is characterised in that first straw is broken into
Powder of straw, then size is more than 100 mesh, is the most by weight: the zymocyte of 0.5-1:2-4:2-4, alginic acid, water are uniform
Be sprayed in the powder of straw by weight 80-90, be uniformly mixed, ferment after sealing, the persistent period is 10-16
My god, fermentation Stress control is 28-35 DEG C in 0.3-0.5MPa, temperature, pH 6-7；After having fermented, by weight 2-4:0.5-1
Add humic acids and formula bacterium, be mixed into final products.
Agriculture microorganism formulation preparation method for soil in protected field conditioning the most according to claim 4, it is characterised in that
Described zymocyte is yeast, bacillus bifidus and bacillus acidophilus, and its mass ratio is 20-30:30-40:20-35.
Agriculture microorganism formulation preparation method for soil in protected field conditioning the most according to claim 4, it is characterised in that
Described formula bacterium includes bacillus subtilis, gel-shaped bacillus, bacillus megaterium, aztobacter sp, its quality
Ratio is 30:15:12:12.
Agriculture microorganism formulation preparation method for soil in protected field conditioning the most according to claim 6, it is characterised in that
Described bacillus subtilis: carry out bacillus subtilis cultivation under aerobic environment, is 3-5% for inoculum concentration, cultivates 12-16 little
Time, pH value 7.0-7.2, temperature 35 DEG C；Culture fluid is designated as peptone 1.0%, yeast powder 0.5%, starch 3%, NaCl by weight
1.0%, the MnSO of 25% concentration40.2%, Na2HPO40.2%, MgSO40.1%, surplus is 1000ml distilled water.
Agriculture microorganism formulation preparation method for soil in protected field conditioning the most according to claim 6, it is characterised in that
Described gel-shaped bacillus: carry out gel-shaped bacillus cultivation under aerobic environment, is 3-5% for inoculum concentration, cultivates 35-
45 hours, pH value 7.5-8.0, temperature 37 DEG C；Culture fluid is designated as molasses 3%, bean cake powder 0.6%, MgSO by weight4·7H2O
0.15%, K2HPO40.05%, CaCO30.2%, surplus is 1000ml distilled water.
Agriculture microorganism formulation preparation method for soil in protected field conditioning the most according to claim 6, it is characterised in that
Described bacillus megaterium: carry out bacillus megaterium cultivation under aerobic environment, is 3-5% for inoculum concentration, cultivates 30-36 little
Time, pH value 7.0-7.2, temperature 30 DEG C；Culture fluid is designated as starch 2%, bean cake powder 4.6%, KH by weight2PO40.2%,
Na2HPO40.05%, MgSO4·7H2O 0.05%, MnSO40.05%, surplus is 1000ml distilled water.
Agriculture microorganism formulation preparation method for soil in protected field conditioning the most according to claim 6, its feature exists
In, described aztobacter sp: carry out aztobacter sp cultivation under aerobic environment, is 3-5% for inoculum concentration, cultivates
24-36 hour, pH value 7.0-7.2, temperature 28 DEG C；Culture fluid is designated as molasses 2%, KH2PO40.05%, MgSO4 by weight
7H2O 0.05%, CaCO3 0.05%, NaCl 0.02%, surplus is 1000ml distilled water.
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Cited By (2)
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| CN109730089A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of microorganism formulation and preparation method thereof promoting plant root growth |
| CN110407638A (en) * | 2019-09-05 | 2019-11-05 | 大连海微生物有限公司 | A kind of microbial bacteria agglomeration-wrapping dry-proof pelleting method |
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| CN104829398A (en) * | 2015-05-19 | 2015-08-12 | 楚长福 | Organic soil improvement fertilizer |
| CN105237289A (en) * | 2015-09-14 | 2016-01-13 | 江苏国瑞绿色食品有限公司 | Multifunctional compound fertilizer |
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| CN102633540A (en) * | 2012-05-04 | 2012-08-15 | 无锡市路华肥料科技有限公司 | Bio-organic fertilizer and production process thereof |
| CN104829398A (en) * | 2015-05-19 | 2015-08-12 | 楚长福 | Organic soil improvement fertilizer |
| CN105237289A (en) * | 2015-09-14 | 2016-01-13 | 江苏国瑞绿色食品有限公司 | Multifunctional compound fertilizer |
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