CN110407638A - A kind of microbial bacteria agglomeration-wrapping dry-proof pelleting method - Google Patents
A kind of microbial bacteria agglomeration-wrapping dry-proof pelleting method Download PDFInfo
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- CN110407638A CN110407638A CN201910836511.XA CN201910836511A CN110407638A CN 110407638 A CN110407638 A CN 110407638A CN 201910836511 A CN201910836511 A CN 201910836511A CN 110407638 A CN110407638 A CN 110407638A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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Abstract
The present invention provides a kind of microbial bacteria agglomeration-wrapping dry-proof pelleting method, comprising the following steps: magnesium sulfate monohydrate, anhydrous zinc sulfate, silico-calcium magnesium oxide, biological bacteria are uniformly mixed obtained raw material 2, raw material 2 is served pelletizer and is granulated；Milk powder, Dextrose Monohydrate, chitosan and sodium glutamate are dissolved into biological bacteria bacterium solution, raw material 1 is made；Raw material 1 is uniformly sprayed onto 2 surface of raw material, while rotating circular disc, it is suitable to granulating size, it directly puts in storage, takes out after hardening 24-48h, dispensed after sieving.Compared with prior art, microbial bacteria agglomeration-wrapping dry-proof pelleting method provided by the invention without drying can intragranular water content drop to 20% or less, the related technical requirement of national GB20287-2006 is fully achieved, to reduce high temperature loss of the microbial inoculum in granulation process, improve survival rate, granulation cost is saved, and improves product commodity.
Description
Technical field
The present invention relates to agriculture field more particularly to a kind of microbial bacteria agglomeration-wrapping dry-proof pelleting methods.
Background technique
Microbial manure refers to a kind of particular product agent containing active microorganism, is applied to that spy can be obtained in agricultural production
Fixed fertilizer effect.After microbial manure is manured into soil, using the vital movement of microorganism by the inertia nitrogen transformation in air
For the ionic state nitrogen that crop can directly absorb, the inorganic matter of indissoluble in soil is become into soluble inorganic matter and is increased in soil
Available N and P, potassium content；The substance that crop cannot directly utilize from soil is converted to the substance that can be absorbed and used,
Manufacture and assistance crops absorb nutrition, improve crop nutritional condition, inhibit the activity of pathogen, enhance crop disease-resistant and anti-morning
Ability；Agricultural microbial agent appropriately using can be improved agricultural output, improve quality of agricultural product, reduce fertilizer amount, drop
Low cost, is preserved the ecological environment at improvement soil.Microbial bacterial agent mostly uses common drying prilling at present, under the technique
Viable bacteria easy in inactivation, it is difficult to guarantee number of viable, while pellet moisture is higher, seed manure caking phenomenon easily occurs with sowing time, limits
The use and effect of product.Cai Jin stone crosses addition regulating material to control the moisture in granulation process, but this method is not put yet
De- drying prilling, viable bacteria easy in inactivation during the drying process.Number of patent application is CN201210105105.4, entitled
A kind of composite microbiological fertilizer and preparation method thereof, which discloses, a kind of improves composite microbe fertilizer with microcapsule embedded mode
Microbial survival rate in material, to improve the quality of composite microbiological fertilizer, but this method later period is granulated in treatment process, still not
It is evitable to have used drying technology.
The cited paper of the above-mentioned prior art are as follows:
A kind of preparation of microorganism particle microbial inoculum [J] agricultural of Cai Jinxin and science and technology, 2018,38 (03), 45-47.
Summary of the invention
Present invention solves the technical problem that being to utilize stoving process in existing microbial bacterial agent granulation process, viable bacteria is easy
Inactivation, it is difficult to guarantee the defect of number of viable.
In order to solve the above-mentioned technical problem, reunite the purpose of the present invention is to provide a kind of microbial bacteria-wrap up no drying
Prilling process.
Specifically, the present invention is to solve technical problem by following technical proposals:
The present invention provides a kind of microbial bacteria agglomeration-wrapping dry-proof pelleting methods, comprising the following steps:
Magnesium sulfate monohydrate, anhydrous zinc sulfate, silico-calcium magnesium oxide, biological bacteria are uniformly mixed obtained raw material 2, by raw material 2
Serve pelletizer granulation；Milk powder, Dextrose Monohydrate, chitosan and sodium glutamate are dissolved into biological bacteria bacterium solution, raw material is made
1；Raw material 1 is uniformly sprayed onto 2 surface of raw material, while rotating circular disc, it is suitable to granulating size, it directly puts in storage, after hardening 24-48h
It takes out, moisture is down to 20% hereinafter, dispensing after sieving.
Preferably, in the raw material 2 according to the mass fraction, magnesium sulfate monohydrate be 10-30 parts, anhydrous zinc sulfate 5-10
Part, silico-calcium magnesium oxide are 1-10 parts, biological bacteria is 2-20 parts, and wherein the viable count of biological bacteria is 100-1000cfu.
Preferably, the biological bacteria added in the raw material 2 is withered grass formula bacillus, bacillus licheniformis, gel-shaped bud
One of spore bacillus, bacillus amyloliquefaciens, Penicillium notatum are a variety of.
Preferably, in the raw material 1 according to the mass fraction, milk powder is 10-100 parts, Dextrose Monohydrate is 10-30 parts, shell
Glycan is 0.5-10 parts, sodium glutamate is 0.1-10 parts, and biological bacteria bacterium solution is by the way that 0.5-10 parts of biological bacterias are dissolved in 500-
It is made in 900 parts of pure water or deionized water, wherein the viable count of biological bacteria is greater than 1000cfu.
Preferably, the biological bacteria added in the raw material 1 be one of lactic acid bacteria, Bifidobacterium, streptococcus thermophilus or
It is a variety of.
Preferably, the mass ratio of the raw material 1 and raw material 2 is 1:(3-20), it is furthermore preferred that the raw material 1 and raw material 2
Mass ratio is 1:(5-10).
Preferably, the silico-calcium magnesium oxide is one of calcium oxide, magnesia, silica or a variety of.
Another object of the present invention is to provide the method in the agriculture containing viable bacteria such as microbial bacterial agent biological organic fertilizer
Application in industry fertilizer.
Wherein, " milk powder " described herein refers to the skimmed milk power that market is sold, without other specific particular/special requirements.
Compared with prior art, microbial bacteria agglomeration-wrapping dry-proof pelleting method provided by the invention is existed using addition
Raw material in material carries out biological respinse and hydration reaction, and the part free water in granular fertilizer is changed into water intracellular and crystallization
Water, without drying can intragranular water content drop to 20% hereinafter, the related skill of national GB20287-2006 is fully achieved
Art index request improves survival rate, saves granulation cost, and mention to reduce high temperature loss of the microbial inoculum in granulation process
High product commodity.
Specific embodiment
Specifically, the present invention improves survival rate, reduces aqueous to reduce high temperature loss of the microbial inoculum in granulation process
Amount, mainly provides a kind of microbial bacteria agglomeration-wrapping dry-proof pelleting method, specifically, in a preferred embodiment,
The present invention is achieved by the steps of:
Magnesium sulfate monohydrate, anhydrous zinc sulfate, silico-calcium magnesium oxide, biological bacteria are uniformly mixed obtained raw material 2, by raw material 2
Serve pelletizer granulation；Milk powder, Dextrose Monohydrate, chitosan and sodium glutamate are dissolved into biological bacteria bacterium solution, raw material is made
1；Raw material 1 is uniformly sprayed onto 2 surface of raw material, while rotating circular disc, it is suitable to granulating size, it directly puts in storage, after hardening 24-48h
It takes out, moisture is down to 20% hereinafter, dispensing after sieving.
Implementation process and technical effect of the invention are illustrated below by specific embodiment, those skilled in the art should
Understanding, this is understood not to the limitation to scope of the invention as claimed, while it is noted that, it is mentioned in the present invention
To the addition number of each substance be mass parts.
Specific raw material, reagent and equipment are commercially available used in embodiment and comparative example.
In mentioned microorganism bacterium agglomeration-wrapping dry-proof pelleting method, the biological respinse and hydration reaction packet that are occurred
It includes:
CaO+H2O=Ca (OH)2
MgO+H2O=Mg (OH)2
MgSO4.H2O+6H2O=MgSO4.7H2O
ZnSO4+H2O=ZnSO4.H2O
Lactic acid bacteria and Bifidobacterium utilize milk powder, glucose and MgSO4.7H2O and water carry out biofermentation reactive absorption
Moisture generates lactic acid and its metabolite.By biological respinse, free water is changed into intracellular water, passes through solid in situ
Fermentation reaction, embedding of carrying disease germs, biological diminishing.
Colony counting method is used to the measurement of survival rate of biological bacteria in embodiment and comparative example herein, it is raw according to institute's micrometer
The culture medium that object and type selection are suitable for calculates viable count using 5 tube method of most probable number MPN (MPN)；Survey to water content is granulated
It is specific to determine method are as follows: empty aluminium box is placed in 105 DEG C of ± 2 DEG C of drying 0.5h in drying box, weighs after cooling and records empty aluminium box
Quality.Then 2 parts of parallel samples are weighed, every part 20.00 grams (being accurate to 0.01g), is separately added into aluminium box and records quality.It will
The aluminium box for installing sample is placed in 105 DEG C of ± 2 DEG C of drying 4h~6h in drying box.It is laggard that taking-up is placed in drier cooling 20min
Row weighing.Moisture content result is the average value measured twice, and numerical value is indicated with %.
In the specification and in the claims, term " includes " and "comprising" are open terms, and are understood as
Refer to " including, but are not limited to ... ".These terms cover more limited term " substantially by ... form " and " by ...
Composition ".
Embodiment 1
By 7 parts of the mixture of 25 parts of magnesium sulfate monohydrate, 5 parts of anhydrous zinc sulfate, calcium oxide and silica, withered grass formula gemma
Bacillus and bacillusmusilaginosiengineering, 10 parts of mixture of bacillus amyloliquefaciens be uniformly mixed raw material 2 be made, raw material 2 is served
Pelletizer is granulated；10 parts of milk powder, 10 parts of Dextrose Monohydrate, 1 part of chitosan and 1 part of sodium glutamate are dissolved into biological bacteria bacterium solution
In, raw material 1 is made, wherein biological bacteria bacterium solution is by the way that 2 parts of lactic acid bacterias, 5 parts of Bifidobacteriums are dissolved in 500 parts of deionized waters
Biology mixed bacteria liquid obtained, the viable count of biological bacteria are greater than 1000cfu；Raw material 1 is uniformly sprayed onto 2 surface of raw material, wherein
The mass ratio of raw material 1 and raw material 2 is 1:3, while rotating circular disc, suitable to granulating size, directly puts in storage, takes out after hardening 48h
Moisture is down to 19%, dispenses after sieving.
It is detected living bacteria count survival rate and reaches 98%, pellet hardness is good, and product viable count is kept after setting 1 year
90% stablizes.
Embodiment 2
By 15 parts of magnesium sulfate monohydrate, 10 parts of anhydrous zinc sulfate, 10 parts of magnesia, withered grass formula bacillus and lichens gemma bar
20 parts of mixture of bacterium are uniformly mixed obtained raw material 2, and raw material 2 is served pelletizer and is granulated；By 30 parts of milk powder, Dextrose Monohydrate 15
Part, 3 parts of chitosan and 5 parts of sodium glutamate be dissolved into biological bacteria bacterium solution, raw material 1 is made, wherein biological bacteria bacterium solution is by by 5
Part lactic acid bacteria, 3 parts of streptococcus thermophilus are dissolved in biology mixed bacteria liquid obtained, the viable count of biological bacteria in 800 parts of pure water
Greater than 1000cfu；Raw material 1 is uniformly sprayed onto 2 surface of raw material, wherein the mass ratio of raw material 1 and raw material 2 is 1:5, while rotating circle
Disk, it is suitable to granulating size, it directly puts in storage, hardening takes out moisture afterwards for 24 hours and is down to 20%, dispenses after sieving.
It is detected living bacteria count survival rate and reaches 95%, pellet hardness is good, and product viable count is kept after setting 1 year
90% stablizes.
Embodiment 3
By 7 parts of 20 parts of magnesium sulfate monohydrate, 9 parts of anhydrous zinc sulfate, calcium oxide and magnesia mixture, withered grass formula gemma bar
Bacterium, 3 parts of Mixed Microbes of bacillus licheniformis and bacillus amyloliquefaciens be uniformly mixed raw material 2 be made, raw material 2 is served into pelletizer
It is granulated；50 parts of milk powder, 18 parts of Dextrose Monohydrate, 7 parts of chitosan and 4 parts of sodium glutamate are dissolved into biological bacteria bacterium solution, are made
Raw material 1, wherein biological bacteria bacterium solution is by the way that 5 parts of streptococcus thermophilus, 5 parts of Bifidobacteriums are dissolved in 700 parts of deionized waters and are made
The viable count of the biological mixed bacteria liquid obtained, biological bacteria is greater than 1000cfu；Raw material 1 is uniformly sprayed onto 2 surface of raw material, Central Plains
Material 1 and the mass ratio of raw material 2 are 1:10, while rotating circular disc, suitable to granulating size, directly put in storage, take out water after hardening 48h
Divide and be down to 18%, is dispensed after sieving.
It is detected living bacteria count survival rate and reaches 96%, pellet hardness is good, and product viable count is kept after setting 1 year
90% stablizes.
Embodiment 4
By 30 parts of magnesium sulfate monohydrate, 6 parts of anhydrous zinc sulfate, magnesia and 4 parts of silica mixture, bacillus licheniformis
13 parts are uniformly mixed obtained raw material 2, and raw material 2 is served pelletizer and is granulated；By 100 parts of milk powder, 30 parts of Dextrose Monohydrate, chitosan
10 parts and 7 parts of sodium glutamate are dissolved into biological bacteria bacterium solution, and raw material 1 is made, and wherein biological bacteria bacterium solution is by by 2 parts of lactic acid
Bacterium, 5 parts of Bifidobacteriums, 2 parts of streptococcus thermophilus are dissolved in biology mixed bacteria liquid obtained, biological bacteria in 900 parts of deionized waters
Viable count be greater than 1000cfu；Raw material 1 is uniformly sprayed onto 2 surface of raw material, wherein the mass ratio of raw material 1 and raw material 2 is 1:8, together
When rotating circular disc, it is suitable to granulating size, directly put in storage, harden 40h after take out moisture be down to 19%, dispensed after sieving.
It is detected living bacteria count survival rate and reaches 95%, pellet hardness is good, and product viable count is kept after setting 1 year
90% stablizes.
Embodiment 5
By the mixing of 10 parts of magnesium sulfate monohydrate, 5 parts of anhydrous zinc sulfate, 2 parts of calcium oxide, withered grass formula bacillus and Penicillium notatum
18 parts of bacterium are uniformly mixed obtained raw material 2, and raw material 2 is served pelletizer and is granulated；70 parts of milk powder, 20 parts of Dextrose Monohydrate, shell are gathered
6 parts and 10 parts of sodium glutamate of sugar is dissolved into biological bacteria bacterium solution, and raw material 1 is made, and wherein biological bacteria bacterium solution is by by 3 parts of lactic acid
Bacterium, 5 parts of Bifidobacteriums are dissolved in biology mixed bacteria liquid obtained, the viable count of biological bacteria in 500 parts of deionized waters and are greater than
1000cfu；Raw material 1 is uniformly sprayed onto 2 surface of raw material, wherein the mass ratio of raw material 1 and raw material 2 is 1:15, while rotating circular disc,
It is suitable to granulating size, it directly puts in storage, taking-up moisture is down to 20% after hardening 36h, dispenses after sieving.
It is detected living bacteria count survival rate and reaches 96%, pellet hardness is good, and product viable count is kept after setting 1 year
90% stablizes.
Comparative example 1
Each additive and its ratio of addition used in comparative example 1 are same as Example 1.
By 7 parts of the mixture of 25 parts of magnesium sulfate monohydrate, 5 parts of anhydrous zinc sulfate, calcium oxide and silica, withered grass formula gemma
Bacillus and bacillusmusilaginosiengineering, 10 parts of mixture of bacillus amyloliquefaciens be uniformly mixed raw material 2 be made, raw material 2 is served
Pelletizer is granulated；10 parts of milk powder, 10 parts of Dextrose Monohydrate, 1 part of chitosan and 1 part of sodium glutamate are dissolved into biological bacteria bacterium solution
In, raw material 1 is made, wherein biological bacteria bacterium solution is by the way that 2 parts of lactic acid bacterias, 5 parts of Bifidobacteriums are dissolved in 500 parts of deionized waters
Biology mixed bacteria liquid obtained, the viable count of biological bacteria are greater than 1000cfu；Raw material 1 is uniformly sprayed onto 2 surface of raw material, wherein
The mass ratio of raw material 1 and raw material 2 is 1:3, while rotating circular disc, suitable to granulating size, is dried into roller high temperature drying oven
Dry, moisture is down to 19% taking-up, dispenses after sieving.
112 DEG C of drying condition, 30min, through detection living bacteria count survival rate discovery, product viable count maintains 20-35%.
120 DEG C of drying condition, 20min, through detection living bacteria count survival rate discovery, product viable count maintains 20-25%.
The comparative example 1 is compared with embodiment 1 compares, and conventional drying mode leads to viable bacteria easy in inactivation, and number of viable is low.This
Apply for that the method Viable detection provided is high, using the agricultural fertilizer significant effect made from the method containing viable bacteria, there are big rule
The market value of mould application.
The above described is only a preferred embodiment of the present invention, being not that the invention has other forms of limitations, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But without departing from the technical solutions of the present invention, according to the technical essence of the invention to above embodiments institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection scope of technical solution of the present invention.
Claims (9)
1. a kind of microbial bacteria agglomeration-wrapping dry-proof pelleting method, comprising the following steps: by magnesium sulfate monohydrate, anhydrous sulphur
Sour zinc, silico-calcium magnesium oxide, biological bacteria are uniformly mixed obtained raw material 2, and raw material 2 is served pelletizer and is granulated；By milk
Powder, Dextrose Monohydrate, chitosan and sodium glutamate are dissolved into biological bacteria bacterium solution, and raw material 1 is made；Raw material 1 is uniform
It is sprayed onto 2 surface of raw material, while rotating circular disc, it is suitable to granulating size, it directly puts in storage, is taken out after hardening 24-48 h,
It is dispensed after sieving.
2. the microbial bacteria agglomeration-wrapping dry-proof pelleting method as described in claim 1, which is characterized in that the original
In material 2 according to the mass fraction, magnesium sulfate monohydrate is 10-30 parts, anhydrous zinc sulfate is 5-10 parts, silicon-calcium-magnesium oxidation
Object is 1-10 parts, biological bacteria is 2-20 parts, and wherein the viable count of biological bacteria is 100-1000 cfu.
3. the microbial bacteria agglomeration-wrapping dry-proof pelleting method as described in claim 1 or 2, which is characterized in that institute
Stating the biological bacteria added in raw material 2 is withered grass formula bacillus, bacillus licheniformis, bacillusmusilaginosiengineering, solution starch
One of bacillus, Penicillium notatum are a variety of.
4. the microbial bacteria agglomeration-wrapping dry-proof pelleting method as described in claim 1, which is characterized in that the original
In material 1 according to the mass fraction, milk powder is 10-100 parts, Dextrose Monohydrate is 10-30 parts, chitosan 0.5-10
Part, sodium glutamate are 0.1-10 parts, and biological bacteria bacterium solution is by the way that 0.5-10 parts of biological bacterias are dissolved in 500-900 parts
It is made in pure water or deionized water, wherein the viable count of biological bacteria is greater than 1000 cfu.
5. the microbial bacteria agglomeration-wrapping dry-proof pelleting method as described in claim 1, which is characterized in that the original
The biological bacteria added in material 1 is one of lactic acid bacteria, Bifidobacterium, streptococcus thermophilus or a variety of.
6. the microbial bacteria agglomeration-wrapping dry-proof pelleting method as described in claim 1, which is characterized in that the original
The mass ratio of material 1 and raw material 2 is 1:(3-20).
7. the microbial bacteria agglomeration-wrapping dry-proof pelleting method as described in claim 6, which is characterized in that the original
The mass ratio of material 1 and raw material 2 is 1:(5-10).
8. the microbial bacteria agglomeration-wrapping dry-proof pelleting method as described in claim 1, which is characterized in that the silicon
Calcium and magnesium oxides are one of calcium oxide, magnesia, silica or a variety of.
9. microbial bacteria agglomeration-wrapping dry-proof pelleting method a method as claimed in any one of claims 1-8 is in microbial bacterial agent biology
Application in the agricultural fertilizers containing viable bacteria such as organic fertilizer.
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