CN101935248B - Bentonite coated complex microbial inoculant as well as preparation method and application thereof - Google Patents
Bentonite coated complex microbial inoculant as well as preparation method and application thereof Download PDFInfo
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- CN101935248B CN101935248B CN2009101959880A CN200910195988A CN101935248B CN 101935248 B CN101935248 B CN 101935248B CN 2009101959880 A CN2009101959880 A CN 2009101959880A CN 200910195988 A CN200910195988 A CN 200910195988A CN 101935248 B CN101935248 B CN 101935248B
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- wilkinite
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- 238000002360 preparation method Methods 0.000 title claims abstract description 19
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- 239000002245 particle Substances 0.000 claims description 33
- 239000002068 microbial inoculum Substances 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 16
- 239000011248 coating agent Substances 0.000 claims description 14
- 238000000576 coating method Methods 0.000 claims description 14
- 241000894006 Bacteria Species 0.000 claims description 13
- 238000010521 absorption reaction Methods 0.000 claims description 10
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- 239000002689 soil Substances 0.000 abstract description 30
- 238000004519 manufacturing process Methods 0.000 abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 12
- 210000003608 fece Anatomy 0.000 abstract description 7
- 239000010871 livestock manure Substances 0.000 abstract description 7
- 239000002367 phosphate rock Substances 0.000 abstract description 7
- 244000063299 Bacillus subtilis Species 0.000 abstract description 5
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- 241000881860 Paenibacillus mucilaginosus Species 0.000 abstract description 5
- 239000007633 bacillus mucilaginosus Substances 0.000 abstract description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 2
- 230000003139 buffering effect Effects 0.000 abstract 1
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- 240000003768 Solanum lycopersicum Species 0.000 description 19
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- 229910021536 Zeolite Inorganic materials 0.000 description 9
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 9
- 238000005469 granulation Methods 0.000 description 9
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- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical group O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 8
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Abstract
The invention relates to a bentonite coated complex microbial inoculant as well as a preparation method and an application thereof. The bentonite coated complex microbial inoculant comprises the following components: 68-76.5% of powdery strain agent, 8-9% of ground phosphate rock, 4-4.5% of median and trace elements and 10-20% of bentonite, wherein the powdery strain agent is formed by mixing bacillus subtilis, bacillus megaterium and bacillus mucilaginosus; and the median and trace elements comprise zinc sulfate, magnesium sulfate and borax. The invention also provides a preparation method and an application of the bentonite coated complex microbial inoculant. The method for producing complex microbial inoculant granules by using the bentonite coated agent can maintain the quality and the fertilizer efficiency of the microbial inoculant, the buffering performance of soil can be enhanced, the soil structure can be improved, the characteristics of water manure, air heat and the like of the soil are coordinated, and the invention also has great importance to environment protection.
Description
[technical field]
The present invention relates to a kind of complex micro organism fungicide of dressing, specifically, is complex micro organism fungicide about a kind of wilkinite dressing and its preparation method and application.
[background technology]
Along with the progress of science, it is reasonable that the agricultural fertilizer technology more and more is tending towards.Using in a large number of chemical fertilizer, so that soil compaction, deteriorating water quality, and utilization rate of fertilizer is low, and agricultural-food and environment have been caused pollution in various degree, and Sustainable development how to keep China's agricultural has caused the great attention of governments at all levels.Particularly after the China joined WTO, China's agricultural is subject to severe challenge, develop foreign exchange earning, and agricultural must be carried forward vigorously the development of green food and pollution-free food, as the microbial fertilizer of one of production pollution-free food production data, become Ministry of Agriculture's emphasis and promoted product.Microbial fertilizer is by be supplemented with the beneficial microorganism bacterium to soil, by its decomposition-reduction effect, solves seriously the hardening of soil, chemical fertilizer exceeds standard, degradation series of problems under the ventilation property that seeps water; Simultaneously, can suppress soil-borne disease, strengthen the activity of soil, promote crop root to absorb nutrient, improve immunizing power, the resistance against diseases of crop, fundamentally improve quality and the grade of food.The tool authoritative department is estimated: in 5 years of future, the annual requirement of microbial fertilizer will reach 30% of fertilizer amount, market capacity will reach the forty-two million ton, and 1,000,000 tons of present year output less thaies of China's microbial fertilizer, needs that far away can not satisfying the market.
Microbiobacterial agent product in the market is mainly take powder-like product as main, number of viable is more than 200,000,000/gram, except being used for producing the composite microbiological fertilizer, can also directly apply to the farmland and increase the soil quantity of useful microbe, Promoting plant growth, strengthen the resistance against diseases of crop, improve output, improve quality of agricultural product etc.But there are two problems in the direct use of powdery microbiobacterial agent product: the one, and it is mechanical planting and fertilising that there are a lot of places in the north, powdery microbial inoculum product can't carry out mechanical fertilization; The 2nd, when being used in conjunction with other granulated fertilizer, can't mix, above two problems have directly restricted the big area of microbiobacterial agent on a lot of zones and crop and have used.Although the granule product of some microbiobacterial agents on market, occurred, the problem that granule strength is inadequate after the ubiquity granulation aborning, particle is inhomogeneous and the rear bacterium number of oven dry reduces.
In order to overcome the above problems, to produce the complex micro organism fungicide granule with the way of wilkinite dressing microbial inoculum and can play good effect.Wilkinite has preferably wetting ability, water swellability and cohesiveness, belong to hydrophilic clay of laminar silicate mineral, maintenance to fertiliser granulates ultimate compression strength has obvious effect, can bond on microbe granular microbial inoculum surface preferably, dressing is on the microbiobacterial agent surface uniformly, because it has preferably cohesiveness and moisture is had very strong adsorptivity, has following special role with wilkinite dressing microbiobacterial agent:
1, increases the ultimate compression strength of microorganism particle microbial inoculum, in Product transport and application, be difficult for fragmentation, be conducive to the use of product, increased the commodity of product;
2, keep the relatively stable of the interior environment of dressing microbial inoculum, prevent from causing owing to moisture loss the reduction of microorganism live bacteria quantity, guaranteed quality product;
3, be applied to soil after, wilkinite can reduce the soil relative density, improve the water content of soil, moisture loss is slow, the length of holding time, keep moisture content in the soil can play unusual vital role for the arid area, the damp soil environment can promote again microbial inoculum microorganism amount reproduction in soil relatively, improves the fertilizer efficiency of product;
4, since the granular bacteria preparation outsourcing one deck wilkinite; except can using separately, can mix with compound manure and the simple substance granular chemical fertilizer of high-content, microbial inoculum can not be in direct contact with it; can not affect microbic activity, can be used in the production of bulk blended fertilizer and formula fertilizer.
China's bentonite resource is very abundant, approximately has 1,700,000,000 tons, and widely distributed, has all found Bentonite Deposits at 23 province and municipalities in the whole nation at present, for the application of wilkinite mineral products resource provides favourable condition.
The above analysis as seen; way with wilkinite dressing microbial inoculum is produced the use function that the complex micro organism fungicide granule has not only increased product; commodity-type and added value; the quality and the fertilizer efficiency that have also kept microbiobacterial agent; and wilkinite itself is to the people; poultry; plant toxicological harmless and corrosive nature; non-stimulated to human body skin; to nerve; respiratory system is without impact; simultaneously; it can also strengthen the shock-absorbing capacity of soil; improve Soil structure, coordinated the characteristics such as liquid manure gas heat of soil, the harmful element in the absorption soil; alleviate the pollution of soil, in environment protection, also have a very big significance.
[summary of the invention]
The objective of the invention is provides a kind of complex micro organism fungicide of wilkinite dressing for deficiency of the prior art.
Again one purpose of the present invention is that the preparation method of the complex micro organism fungicide of wilkinite dressing is provided.
Another purpose of the present invention is that the purposes of the complex micro organism fungicide of wilkinite dressing is provided.
For achieving the above object, the technical scheme taked of the present invention is:
A kind of complex micro organism fungicide of wilkinite dressing, by weight percentage, the complex micro organism fungicide of this wilkinite dressing is composed of the following components:
Powdery inoculum agent 68-76.5%
Ground phosphate rock 8-9%
Middle micro-4-4.5%
Wilkinite 10-20%,
Described powdery inoculum agent is by subtilis, bacillus megaterium, and bacillusmusilaginosiengineering mixes, and described middle trace element is by zinc sulfate, and sal epsom and borax form.
Be preferably, by weight percentage, the complex micro organism fungicide of this wilkinite dressing is composed of the following components:
Powdery inoculum agent 68-76.5%
Ground phosphate rock 8-9%
Zinc sulfate 1.6-1.8%
Sal epsom 1.6-1.8%
Borax 0.8-0.9%
Wilkinite 10-20%.
Be preferably, by weight percentage, the complex micro organism fungicide of this wilkinite dressing is composed of the following components:
Powdery inoculum agent 72.25%
Ground phosphate rock 8.5%
Zinc sulfate 1.7%
Sal epsom 1.7%
Borax 0.85%
Wilkinite 1 5%.
For realizing above-mentioned second purpose, the technical scheme that the present invention takes is:
A kind of preparation method of complex micro organism fungicide of wilkinite dressing, the method may further comprise the steps:
The preparation of a, complex micro organism fungicide: with subtilis, the independent fermentative production of bacillus megaterium and bacillusmusilaginosiengineering, bacterium liquid after the fermentation adsorbs respectively, and the single microbial inoculum after three kinds of absorption is mixed, pulverizes according to 2: 2: 1 ratio, obtains complex micro organism fungicide;
The preparation of the complex micro organism fungicide of b, wilkinite dressing: batching-pulverizing-granulation-dressing-cooling-screening-finished product; wherein dressing is coating pelletizing; in tablets press, evenly add wilkinite; make wilkinite evenly wrap in the surface of microbial inoculum particle; coating thickness is 1mm, and particle diameter reaches placing behind the 3-4mm.
For realizing above-mentioned the 3rd purpose, the technical scheme that the present invention takes is:
A kind of complex micro organism fungicide of wilkinite dressing, the application of as fertilizer sources in the agricultural crops growth, the complex micro organism fungicide of described wilkinite dressing is by weight percentage, and is composed of the following components:
Powdery inoculum agent 68-76.5%
Ground phosphate rock 8-9%
Middle micro-4-4.5%
Wilkinite 10-20%,
Described powdery inoculum agent is by subtilis, bacillus megaterium, and bacillusmusilaginosiengineering mixes, and described middle trace element is by zinc sulfate, and sal epsom and borax form.
Be preferably the application of the complex micro organism fungicide as fertilizer sources of described wilkinite dressing in the growth of agricultural crops nonirrigated farmland.
Know-why of the present invention is, bentonitic major ingredient is montmorillonite, and the crystalline structure of montmorillonite is comprised of two-layer silicon-oxy tetrahedron wafer therebetween one deck alumina octahedral wafer, belongs to 2:1 type layer silicate mineral.Its theoretical chemistry formula is Na
x(H
2O)
4{ Al
2[Al
xSi
4-xO
10] (OH)
2, total anionic charge [O in half structure cell
10(OH)
2] be 22, the positively charged ion number that exists in the octahedron is 2, the positively charged ion number that exists in the tetrahedron is 4.There is the positively charged ion isomorphous replacement in general silicon-oxy tetrahedron and the alumina octahedral, when the displacement positively charged ion is low price, makes structure increase the negative charge of equivalent, compensated by the interlayer Liquidity limit.Form electric dipole between positively charged ion and crystal skeleton between the montmorillonite crystal layer, add that bonding force between the montmorillonite crystal layer a little less than, can adsorb polar water molecules, according to cation type and relative humidity, interlayer can adsorb one deck or two-layer water molecules.In addition, also can adsorb certain water molecules at the montmorillonite grain surface, water of constitution is present in the lattice with OH base form; Simultaneously, wilkinite has adsorption and multiple poly-attached form to organism, can form micella, all produces cohesiveness when gathering, flocculation, gel.Therefore, this structural property of cation replacement has determined a series of critical natures of montmorillonite in the montmorillonite lattice, particularly at aspects such as strong absorptive and cohesiveness.In addition, inflatable peeling off between the crystal layer of montmorillonite forms less laminated structure, is easy to form the multilayer coating structure fertilizer nutrient; Moreover, the bridging reaction occurs between the lamella of adjacent particle, form network, this has just determined that wilkinite can be used as the good coating material of microbiobacterial agent.
Compare with existing situation, the present invention has following advantage:
1, high-moisture-retention: wilkinite has stronger water-absorbent, swelling property and water retention property, water-intake rate can be up to 100-240%, volume energy with absorb water and minute swell 10-30 doubly.Therefore can keep the relatively stable of ambient moisture in the microbiobacterial agent of dressing, keep for a long time the biological activity of microbial fertilizer, improve the quality product of microbial fertilizer; Simultaneously, can reduce the soil relative density after using wilkinite, improve the fractal dimension of soil, can suitably improve the water content of punja, reduce moisture loss, the water retention property that strengthens soil is played an important role.
2, ultimate compression strength is high: after microbiobacterial agent is wrapped by wilkinite, wilkinite has stronger cohesiveness, obviously improved granule strength, prevent microbial inoculum caking and broken, make it keep good free-running property and uniformity coefficient, be conducive to mechanical fertilization and mix use with other granule type compound manure, can greatly improve utilization rate of fertilizer, also be conducive to storage and the transportation of product.
3, strengthen the reclamation result of microbial inoculum: wilkinite is to coordinating the liquid manure gas heat of soil, increase soil fertility and have good effect, can help the formation of Soil structure, improve the fertilizer conservation water retention capacity of soil, improve the hydrothermal condition of soil, so utilize wilkinite dressing microbiobacterial agent can increase the reclamation result of microbiobacterial agent, satisfy crop each vegetative period to the needs of the conditions such as nutrient and moisture, reach the target of high grade and yield of crops.
4, high value added utilization: China's bentonite ore aboundresources, proven reserve are 17.17 hundred million tons, 5.53 hundred million tons of industry, produce per year more than 1,100,000 tons, bentonitic research was mainly concentrated on industrial aspect both at home and abroad in the past, with its application on agricultural, soil and fertilizer, particularly cooperate the utilization of composite microbiological fertilizer, not only obtain the higher disposal of resources approach of added value, and improved greatly the validity of production and the use procedure of microbial fertilizer, for we provide a brand-new thinking.
[description of drawings]
Accompanying drawing 1 is the production technological process of powdery complex micro organism fungicide.
Accompanying drawing 2 is the complex micro organism fungicide production technological process of wilkinite dressing.
[embodiment]
Below in conjunction with accompanying drawing embodiment provided by the invention is elaborated.
Embodiment 1
1, strain fermentation
With subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), bacillusmusilaginosiengineering (Bacillus mucilaginosus), three bacterial classifications make the number of viable of zymocyte liquid reach hundred million/ml of 25-35 respectively separately through slant culture, shaking table enlarged culturing, seeding tank fermentation and ferment tank;
2, absorption
In the absorption whipping device, use respectively moisture less than 10% the zymocyte liquid of three bacterial classifications, fineness is adsorbed greater than 100 purpose weathered coals, according to bacterium liquid: the ratio of weathered coal=1: 4 (L/kg) is adsorbed, then stir, moisture controlled is not as the criterion with loose the caking 35%;
3, mix
With three kinds respectively with the single microbial inoculum after the weathered coal absorption according to subtilis (Bacillussubtilis): bacillus megaterium (Bacillus megaterium): the part by weight of bacillusmusilaginosiengineering (Bacillusmucilaginosus)=2: 2: 1 mixes; (technological process of production of powdery complex micro organism fungicide can referring to Fig. 1)
4, composite bacteria detects:
The examination and test of products is carried out according to the method for Ministry of Agriculture's industry standard (NY227-1994), and detected result is as follows:
The detected result of table 1 powdery complex micro organism fungicide
5, batching: in capacity is 500 kilograms stirrer, carry out accurate feed proportioning according to the proportioning raw materials of table 2: powdery complex microorganism bacterial classification agent 85%, ground phosphate rock 10% (play cohesive action and increase the phosphorus source), middle micro-5% ratio is fully mixed stirrer stir about 10-15 minute in batching with stirrer;
The production formula of table 2 microbiobacterial agent granulation
6, granulation: enter granulating disc and carry out granulation, enter second granulating disc after making the particle diameter of granulation reach 2-3mm;
7, dressing: second disk is coating pelletizing, evenly adds wilkinite in tablets press, makes wilkinite evenly wrap in the surface of microbial inoculum particle, and coating thickness is about 1mm, and particle diameter reaches placing behind the 3-4mm;
The bentonitic main chemical of table 3
The production formula of table 4 microbiobacterial agent particle
8, oven dry: the microbial inoculum particle behind the dressing is dried, and the oven dry of low temperature Wind Volume cold wind is taked in oven dry, and the bake out temperature inlet temperature is at 120-150 ℃, and temperature of charge is not higher than 80 ℃ in dram;
9, cooling: material cools off in cooling cylinder, makes the cooling cylinder temperature out be lower than 35 ℃;
10, screening: with sieve apparatus particle is sieved, particle diameter is controlled at 3-4mm;
11, finished product: particle diameter is that finished product is packed at the microbial inoculum particle of 3-4mm.(the complex micro organism fungicide technological process of production of wilkinite dressing can referring to Fig. 2)
Product quality inspection
The examination and test of products is carried out according to the method for Ministry of Agriculture's industry standard (NY227-1994), to having carried out measure (see Table 5) as carrier adsorption and wilkinite as the indexs such as living bacteria count in the complex micro organism fungicide of granule coating material with weathered coal:
The detected result of table 5 complex micro organism fungicide
Can find out from detected result: with the coating material of wilkinite as microbiobacterial agent, its detected result meets Ministry of Agriculture's industry standard (NY227-1994), and obviously improved grain graininess and ultimate compression strength, can draw with wilkinite dressing microbiobacterial agent production complex micro organism fungicide particle product effect remarkable.
Embodiment 2
In the complex micro organism fungicide preparation of wilkinite dressing, the production formula of microbiobacterial agent particle sees Table 4-A.Other are with embodiment 1.
The production formula of table 4-A microbiobacterial agent particle
Embodiment 3
In the complex micro organism fungicide preparation of wilkinite dressing, the production formula of microbiobacterial agent particle sees Table 4-B.Other are with embodiment 1.
The production formula of table 4-B microbiobacterial agent particle
Embodiment 4
The tomato field test effect
Test is processed:
A: use 5 kg/acres of the complex micro organism fungicide granules (adopting the complex micro organism fungicide of the wilkinite dressing of embodiment 1) of wilkinite dressing, hereinafter to be referred as processing A;
B: use 5 kg/acres of common complex micro organism fungicide granules (adopt the microbiobacterial agent without the wilkinite coating pelletizing, the preparation method removes without step 7 (wilkinite coating pelletizing), and other are with embodiment 1), hereinafter to be referred as treatments B;
C: use separately 5 kg/acres of wilkinites, hereinafter to be referred as processing C (adopt wilkinite directly to use, other are with embodiment 1);
D: blank, hereinafter to be referred as processing D.
Residential quarter area 20m
2, establish 3 repetitions, random district group is arranged.Fertilization mode with granular bacteria preparation adopt spread manuer in holes or ditch spread at crop rhizosphere soils, crop base manure with topdress identical with habits fertilization.Testing data is carried out variance analysis and test of significance thereof with SPSS13.0 software, and Excel adds up, analyzes.
(1) different treatment is on the impact of tomato agronomic shape
Table 6 different treatment is on the impact of agronomic shape
Data are found out from table 6: in four kinds of different treatment impacts on the agronomic shape of crop, the indices of processing A is better than other processing, process A, treatments B and processing C and increased 5.7cm, 4.1cm and 1.1cm than the plant height of processing the D tomato respectively, stem has slightly increased 0.34cm, 0.17cm and 0.09cm, individual plant fruit number increases by 3,1.6 and 0.5, and single fruit weight increases by 32 grams, 13 grams and 9 grams.The particle product that the complex micro organism fungicide of using the wilkinite dressing is described can effectively promote the growth of tomato, have improved the agronomic shape of tomato.
(2) different treatment is on the impact of tomato production
Table 7 different treatment is on the impact of tomato production
Four kinds of different treatment can be found out result's (table 7) of tomato production, process A, treatments B and processing C respectively than the output increased of processing the D tomato 13.6%, 6.5% and 2.4%, illustrate to use with the particle product of the complex micro organism fungicide of wilkinite dressing and can improve preferably tomato production, and effect of increasing production reaches utmost point conspicuous level; Use common complex micro organism fungicide and alone wilkinite also has production-increasing function, but it is obvious not use the effect of increasing production of complex micro organism fungicide of wilkinite dressing.
Can find out from above test-results: the particle product with the complex micro organism fungicide of wilkinite dressing can obviously promote plant growth, increase crop yield.Simultaneously, can play the effect that reduces the wasting of resources, protection of the environment, minimizing fertilizer amount, raising quality of agricultural product.
Embodiment 5
Parallel laboratory test
Parallel laboratory test between the complex micro organism fungicide of the complex micro organism fungicide of wilkinite dressing and zeolite dressing.
The complex micro organism fungicide of wilkinite dressing adopts the complex micro organism fungicide of the wilkinite dressing of embodiment 1.
The zeolite major ingredient is silicon-dioxide, and its chemical constitution is Na
6(Al
6Si
30O
72) 24H
2O is three-dimensional silica four sides crystal and three-dimensional alumina four sides crystal skeleton structure, and structure opening is larger, and crystals has bug hole and the duct of many marshallings, in crystal duct and the hole and with tradable metallic cation and desorption water molecules freely.Ability with the external gas of absorption, moisture, molecule and ion.
The complex micro organism fungicide preparation method of zeolite dressing:
1, strain fermentation
With subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), bacillusmusilaginosiengineering (Bacillus mucilaginosus), three bacterial classifications make the number of viable of zymocyte liquid reach hundred million/ml of 25-35 respectively separately through slant culture, shaking table enlarged culturing, seeding tank fermentation and ferment tank;
2, absorption
In the absorption whipping device, use respectively moisture less than 10% the zymocyte liquid of three bacterial classifications, fineness is adsorbed greater than 100 purpose weathered coals, according to bacterium liquid: the ratio of weathered coal=1: 4 (L/kg) is adsorbed, then stir, moisture controlled is not as the criterion with loose the caking 35%;
3, mix
With three kinds respectively with the single microbial inoculum after the weathered coal absorption according to subtilis (Bacillussubtilis): bacillus megaterium (Bacillus megaterium): the part by weight of bacillusmusilaginosiengineering (Bacillusmucilaginosus)=2: 2: 1 mixes;
4, composite bacteria detects:
The examination and test of products is carried out according to the method for Ministry of Agriculture's industry standard (NY227-1994), and detected result is as follows:
The detected result of table 8 powdery complex micro organism fungicide
5, batching: in capacity is 500 kilograms stirrer, carry out accurate feed proportioning according to the proportioning raw materials of table 2: powdery complex microorganism bacterial classification agent 85%, ground phosphate rock 10% (play cohesive action and increase the phosphorus source), middle micro-5% ratio is fully mixed stirrer stir about 10-15 minute in batching with stirrer;
The production formula of table 9 microbiobacterial agent granulation
6, granulation: enter granulating disc and carry out granulation, enter second granulating disc after making the particle diameter of granulation reach 2-3mm;
7, dressing: second disk is coating pelletizing, evenly adds zeolite in tablets press, makes zeolite evenly wrap in the surface of microbial inoculum particle, and coating thickness is about 1mm, and particle diameter reaches placing behind the 3-4mm;
The production formula of table 10 microbiobacterial agent particle
8, oven dry: the microbial inoculum particle behind the dressing is dried, and the oven dry of low temperature Wind Volume cold wind is taked in oven dry; The bake out temperature inlet temperature is at 120-150 ℃, and temperature of charge is not higher than 80 ℃ in dram;
9, cooling: material cools off in cooling cylinder, makes the cooling cylinder temperature out be lower than 35 ℃;
10, screening: with sieve apparatus particle is sieved, particle diameter is controlled at 3-4mm;
11, finished product: particle diameter is that finished product is packed at the microbial inoculum particle of 3-4mm.
Test is processed:
A: use 5 kg/acres of the complex micro organism fungicide granules (adopting the complex micro organism fungicide of the wilkinite dressing of embodiment 1) of wilkinite dressing, hereinafter to be referred as processing A;
B: use 5 kg/acres of the complex micro organism fungicide granules (adopting the complex micro organism fungicide of the zeolite dressing of embodiment 5) of zeolite dressing, hereinafter to be referred as treatments B;
C: blank, hereinafter to be referred as processing C.
Residential quarter area 20m
2, establish 3 repetitions, random district group is arranged.Fertilization mode with granular bacteria preparation adopt spread manuer in holes or ditch spread at crop rhizosphere soils, crop base manure with topdress identical with habits fertilization.Testing data is carried out variance analysis and test of significance thereof with SPSS13.0 software, and Excel adds up, analyzes.
(1) different treatment is on the impact of tomato agronomic shape
Table 11 different treatment is on the impact of agronomic shape
Data are found out from table 11: in three kinds of different treatment impacts on the agronomic shape of crop, the indices of processing A is better than treatments B and processes C, process A, treatments B and increase 5.7cm and 3.6cm than the plant height of processing the C tomato respectively, the stem rough segmentation does not increase 0.34cm and 0.16cm, strain fruit number increases by 3 and 1.7, single fruit weight increases by 32 grams and 7 grams, illustrate that the complex micro organism fungicide of using the wilkinite dressing than the growth that more can effectively promote tomato with the complex micro organism fungicide of zeolite powder dressing, has improved the agronomic shape of tomato.
(2) different treatment is on the impact of tomato production
Table 12 different treatment is on the impact of tomato production
Three kinds of different treatment can be found out result's (table 12) of tomato production, process A and treatments B respectively than the output increased of processing the C tomato 13.6% and 8.1%, the effect of processing A is better than treatments B, significant difference, illustrate that the complex micro organism fungicide with the wilkinite dressing more can improve tomato production preferably than the complex micro organism fungicide with the zeolite powder dressing, and effect of increasing production reaches utmost point conspicuous level.
The above only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the inventive method; can also make some improvement and replenish, these improvement and replenish and also should be considered as protection scope of the present invention.
Claims (5)
1. the complex micro organism fungicide of the wilkinite dressing of a high granule strength, by weight percentage, the complex micro organism fungicide of this wilkinite dressing is composed of the following components:
Described powdery inoculum agent is mixed by subtilis, bacillus megaterium, bacillusmusilaginosiengineering and weathered coal, and described middle trace element is by zinc sulfate, and sal epsom and borax form.
2. the complex micro organism fungicide of the wilkinite dressing of high granule strength according to claim 1 is characterized in that, by weight percentage, the complex micro organism fungicide of this wilkinite dressing is composed of the following components:
3. the complex micro organism fungicide of the wilkinite dressing of high granule strength according to claim 2 is characterized in that, by weight percentage, the complex micro organism fungicide of this wilkinite dressing is composed of the following components:
4. the preparation method of the complex micro organism fungicide of the wilkinite dressing of arbitrary described high granule strength is characterized in that according to claim 1-3, and the method may further comprise the steps:
The preparation of a, complex micro organism fungicide: with subtilis, the independent fermentative production of bacillus megaterium and bacillusmusilaginosiengineering, bacterium liquid after the fermentation adsorbs respectively, and the single microbial inoculum after three kinds of absorption is mixed, pulverizes according to 2: 2: 1 ratio, obtains complex micro organism fungicide;
The preparation of the complex micro organism fungicide of b, wilkinite dressing: batching-pulverizing-granulation-dressing-cooling-screening-finished product; wherein dressing is coating pelletizing; in tablets press, evenly add wilkinite; make wilkinite evenly wrap in the surface of microbial inoculum particle; coating thickness is 1mm, and particle diameter reaches placing behind the 3-4mm.
5. the application of the complex micro organism fungicide as fertilizer sources of the wilkinite dressing of high granule strength claimed in claim 1 in the growth of agricultural crops nonirrigated farmland.
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| CN103288560B (en) * | 2013-04-03 | 2016-05-18 | 王楠 | A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil |
| CN105084996A (en) * | 2014-05-14 | 2015-11-25 | 黄庆禄 | Preparation and field operation method of ecological planting targeted fertilization drug |
| CN105801240A (en) * | 2016-01-29 | 2016-07-27 | 山东鑫圣源生物工程技术有限公司 | Fertilizer batching microbial agent and preparation method therefor |
| CN105777372B (en) * | 2016-04-05 | 2019-06-21 | 山东宝源生物科技股份有限公司 | Improve the composite microbiological fertilizer and the preparation method and application thereof of crop anti-adversity |
| CN110521734A (en) * | 2019-09-02 | 2019-12-03 | 广东海粤盛农业有限公司 | A kind of peanut seed coating agent |
| CN110419644A (en) * | 2019-09-12 | 2019-11-08 | 菏泽六和饲料有限责任公司 | A kind of Low protein diet and preparation method thereof suitable for broiler early rearing |
| CN113354477A (en) * | 2021-07-28 | 2021-09-07 | 黑龙江神飞生物科技股份有限公司 | Special compound fertilizer rich in microbial agent for soybeans and preparation method thereof |
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| CN1566038A (en) * | 2003-06-25 | 2005-01-19 | 中国科学院大连化学物理研究所 | Biological inorganic complex fertilizer |
| CN101054317A (en) * | 2006-04-13 | 2007-10-17 | 孙明 | Process of producing plant water keeping organic fertilizer |
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