CN106107673A - 一种樱桃酵素的制备方法 - Google Patents
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Abstract
本发明公开一种樱桃酵素的制备方法,樱桃经过前处理、酶解、制基质、发酵、浓缩干燥制得樱桃酵素。樱桃酵素中含有丰富的酶、维生素、矿物质和次级代谢产物等营养成分,具有抗衰老、抗菌消炎、增强机体免疫力、修复机体损伤等功能。
Description
技术领域
本发明涉及一种酵素的制备方法,特别是一种樱桃酵素的制备方法。
背景技术
樱桃被誉为“水果中的钻石”,因为它具有非凡的营养价值,对痛风、关节炎等病有特殊的食疗效果。樱桃含铁量较高,比苹果高20-30倍,铁是合成人体血红蛋白、肌红蛋白的原料,在人体免疫、蛋白质合成、能量代谢等过程中,发挥着重要作用。同时也与大脑及神经功能、衰老过程密切相关。樱桃中同样含有褪黑激素,因此具有双倍的抗衰老作用,是名副其实“美味又美丽”的水果。樱桃含有丰富的蛋白质,维生素A、B、C,还有钾、钙、磷、铁等矿物质以及多种维生素,低热量,高纤维。维生素A比葡萄高4倍,维生素C的含量更高。樱桃采摘后易腐烂,不宜储存,制约了樱桃产业的进一步发展。
酵素,也就是酵母分泌的“代谢产物”,也称为“酶”,是一种生物催化剂,由蛋白质构成。酵素几乎参与所有的身体活动,在它的参与下人体才能维持正常的新陈代谢、自体免疫、修复组织等生理功能。
发明内容
本发明的目的在于解决了樱桃不宜储存的问题,提供一种樱桃酵素的制备方法,酵素中含有丰富的酶、维生素、矿物质和次级代谢产物等营养成分,将樱桃的有效成分完整保留,进一步提高了樱桃的附加值。
一种樱桃酵素的制备方法,按照以下步骤完成:
1、前处理:将成熟的樱桃清洗干净,去核,打浆;
2、酶解:将樱桃浆与水1:2混匀,加入0.05-0.5%酶,于50-60℃酶解2-3h,离心收集酶解液;
3、制基质:测定酶解液可溶性固形物含量,添加蔗糖,使固形物含量达到16-24%,再添加质量分数0.1-0.3%的酵母膏,0.01-0.2%的硫酸镁,0.01-0.1%的磷酸氢二钾,0.01-0.1%的磷酸二氢钾,0.01-0.2%的氯化锌,得发酵基质;
4、发酵:将发酵基质加热100℃灭菌20-30分钟,冷却至室温后,接种0.01-0.2%酵母菌,于28-32℃发酵10-14h,再接种O.01-0.1%乳酸菌,36-38℃下发酵26-28h,最后于6—8℃下后酵22-26h;
5、浓缩干燥:发酵液浓缩后经真空冷冻干燥制成樱桃酵素粉。
本发明所述,酶解所用的酶为480U/g木聚糖酶、500U/g诺维信酶中的一种或者两种的混合物。
本发明的优点在于:经酵母菌和乳酸菌发酵后的樱桃酵素具有良好的抗氧化活性。后酵产香后制得的酵素粉末呈现棕红色,果香浓郁,伴有特殊的发酵香气,酸甜适口,食用特性和功能特性均得到了较大提高。
具体实施方式
下面结合实施例对本发明做进一步描述。
实施例1
一种樱桃酵素的制备方法,按照以下步骤完成:
1、前处理:将成熟的樱桃清洗干净,去核,打浆;
2、酶解:将樱桃浆与水1:2混匀,加入0.07%的480U/g木聚糖酶和0.08%的500U/g诺维信酶,于56℃酶解2.5h,离心收集酶解液;
3、制基质:测定酶解液可溶性固形物含量,添加蔗糖,使固形物含量达到18%,再添加质量分数0.2%的酵母膏,0.1%的硫酸镁,0.05%的磷酸氢二钾,0.05%的磷酸二氢钾,0.1%的氯化锌,得发酵基质;
4、发酵:将发酵基质加热100℃灭菌21分钟,冷却至室温后,接种0.1%酵母菌,于30℃发酵12h,再接种O.05%乳酸菌,37℃下发酵27h,最后于7℃下后酵24h;
5、浓缩干燥:发酵液浓缩后经真空冷冻干燥制成樱桃酵素粉。
以上对本发明进行了详细介绍,以上实施例的说明只是用于帮助理解本发明。本领域的普通技术人员应当理解,可以对本发明的技术方案进行更动、润饰或者等同替换,而不脱离本发明技术方案的精神和范围,其均应属于本发明的权利要求保护范围当中。
Claims (2)
1.一种樱桃酵素的制备方法,按照以下步骤完成:
a、前处理:将成熟的樱桃清洗干净,去核,打浆;
b、酶解:将樱桃浆与水1:2混匀,加入0.05-0.5%酶,于50-60℃酶解2-3h,离心收集酶解液;
c、制基质:测定酶解液可溶性固形物含量,添加蔗糖,使固形物含量达到16-24%,再添加质量分数0.1-0.3%的酵母膏,0.01-0.2%的硫酸镁,0.01-0.1%的磷酸氢二钾,0.01-0.1%的磷酸二氢钾,0.01-0.2%的氯化锌,得发酵基质;
d、发酵:将发酵基质加热100℃灭菌20-30分钟,冷却至室温后,接种0.01-0.2%酵母菌,于28-32℃发酵10-14h,再接种O.01-0.1%乳酸菌,36-38℃下发酵26-28h,最后于6—8℃下后酵22-26h;
e、浓缩干燥:发酵液浓缩后经真空冷冻干燥制成樱桃酵素粉。
2.根据权利要求1所述,酶解所用的酶为480U/g木聚糖酶、500U/g诺维信酶中的一种或者两种的混合物。
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107183685A (zh) * | 2017-05-12 | 2017-09-22 | 燕山大学 | 一种樱桃酵素脂质体的制备方法 |
CN108968038A (zh) * | 2018-06-19 | 2018-12-11 | 上海应用技术大学 | 一种樱桃酵素及其制备方法 |
CN109007798A (zh) * | 2018-07-11 | 2018-12-18 | 伍曾利 | 一种针叶樱桃果渣酵素液、酵素粉及其制备方法 |
CN109260076A (zh) * | 2018-10-19 | 2019-01-25 | 浙江大学自贡创新中心 | 一种樱桃发酵液及其制备方法和在化妆品中的应用 |
CN110477106A (zh) * | 2019-09-26 | 2019-11-22 | 成都康源量子生物技术研究院 | 一种高能量樱桃李酶饮料及其制备方法 |
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CN104686958A (zh) * | 2015-02-28 | 2015-06-10 | 山东绿丰生态农业有限公司 | 樱桃酵素的制备方法 |
CN105166907A (zh) * | 2015-10-10 | 2015-12-23 | 宁波海通食品科技有限公司 | 一种麦苗酵素的快速发酵制备方法 |
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Patent Citations (2)
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CN104686958A (zh) * | 2015-02-28 | 2015-06-10 | 山东绿丰生态农业有限公司 | 樱桃酵素的制备方法 |
CN105166907A (zh) * | 2015-10-10 | 2015-12-23 | 宁波海通食品科技有限公司 | 一种麦苗酵素的快速发酵制备方法 |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107183685A (zh) * | 2017-05-12 | 2017-09-22 | 燕山大学 | 一种樱桃酵素脂质体的制备方法 |
CN108968038A (zh) * | 2018-06-19 | 2018-12-11 | 上海应用技术大学 | 一种樱桃酵素及其制备方法 |
CN109007798A (zh) * | 2018-07-11 | 2018-12-18 | 伍曾利 | 一种针叶樱桃果渣酵素液、酵素粉及其制备方法 |
CN109260076A (zh) * | 2018-10-19 | 2019-01-25 | 浙江大学自贡创新中心 | 一种樱桃发酵液及其制备方法和在化妆品中的应用 |
CN110477106A (zh) * | 2019-09-26 | 2019-11-22 | 成都康源量子生物技术研究院 | 一种高能量樱桃李酶饮料及其制备方法 |
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Application publication date: 20161116 |
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