CN105848746B - 用于层析重复利用的方法 - Google Patents

用于层析重复利用的方法 Download PDF

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Publication number
CN105848746B
CN105848746B CN201480048900.1A CN201480048900A CN105848746B CN 105848746 B CN105848746 B CN 105848746B CN 201480048900 A CN201480048900 A CN 201480048900A CN 105848746 B CN105848746 B CN 105848746B
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buffer
antibody
volumes
protein
polypeptide
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Chinese (zh)
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CN105848746A (zh
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E·马哈延
K·科塔里
J·索
J·韦伯
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B01D15/203—Equilibration or regeneration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J49/00—Regeneration or reactivation of ion-exchangers; Apparatus therefor
    • B01J49/60—Cleaning or rinsing ion-exchange beds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201480048900.1A 2013-09-05 2014-09-05 用于层析重复利用的方法 Active CN105848746B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361874305P 2013-09-05 2013-09-05
US61/874,305 2013-09-05
PCT/US2014/054313 WO2015035180A1 (en) 2013-09-05 2014-09-05 Method for chromatography reuse

Publications (2)

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CN105848746A CN105848746A (zh) 2016-08-10
CN105848746B true CN105848746B (zh) 2019-07-09

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US (2) US20150093800A1 (https=)
EP (1) EP3041593B1 (https=)
JP (5) JP6602765B2 (https=)
KR (1) KR20160050062A (https=)
CN (1) CN105848746B (https=)
CA (1) CA2922832A1 (https=)
MX (1) MX2016002798A (https=)
RU (1) RU2016112549A (https=)
WO (1) WO2015035180A1 (https=)

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CN112646045A (zh) * 2021-01-06 2021-04-13 海默斯（重庆）医学生物技术有限公司 一种重组角蛋白分离纯化方法
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CN113842673B (zh) * 2021-09-07 2023-01-06 济宁海关综合技术服务中心 液相色谱柱再生方法
TW202337900A (zh) * 2022-01-12 2023-10-01 美商再生元醫藥公司 改良使用親和層析的異二聚蛋白質自雜質之解析的方法
CN120693200A (zh) * 2022-12-19 2025-09-23 伊莱利利公司 清洁色谱基质的方法
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Also Published As

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JP2023085273A (ja) 2023-06-20
RU2016112549A (ru) 2017-10-10
CN105848746A (zh) 2016-08-10
EP3041593B1 (en) 2025-01-29
US20190054396A1 (en) 2019-02-21
JP6602765B2 (ja) 2019-11-06
JP2019094334A (ja) 2019-06-20
EP3041593A4 (en) 2017-08-30
MX2016002798A (es) 2016-07-21
CA2922832A1 (en) 2015-03-12
JP2016530533A (ja) 2016-09-29
JP2025186245A (ja) 2025-12-23
KR20160050062A (ko) 2016-05-10
US10940401B2 (en) 2021-03-09
US20150093800A1 (en) 2015-04-02
JP2021155421A (ja) 2021-10-07
WO2015035180A1 (en) 2015-03-12
EP3041593C0 (en) 2025-01-29
EP3041593A1 (en) 2016-07-13

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