CN105766295A - Artificial breeding method for rhododendron huadingense seedlings - Google Patents
Artificial breeding method for rhododendron huadingense seedlings Download PDFInfo
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Abstract
The invention discloses an artificial breeding method for rhododendron huadingense seedlings and belongs to the technical field of rare plant artificial breeding.The method comprises the following steps of fruit collection, seed preservation, seed germination, seedling hardening for the first time, seedling hardening for the second time and seedling transplanting.By means of the artificial breeding method, the germination rate of rhododendron huadingense seeds can be high and exceed 40%, the survival rate of the seedlings is high, the total survival rate can reach 75%, the seedlings which is successfully bred grow well, it is beneficial to protect the rare or endangered species rhododendron huadingense, and it is also beneficial to develop and utilize the plant as an ornamental flower variety.
Description
Technical field
The invention belongs to rare plant artificial breeding technology field, be specifically related to the artificial breeding method of a kind of rare plant China top Cuculus polioephalus nursery stock.
Background technology
China top Cuculus polioephalus (Rhododendronhuadingense) is the distinctive rhododendron in Zhejiang, China top Cuculus polioephalus is mainly distributed on Tiantai County in Zhejiang Province Mount Huading, being positioned between height above sea level 750 ~ 950m, winding greatly in zhejiang pan'an also have NATURAL DISTRIBUTION, and areal area is narrow and small.The bud of China top Cuculus polioephalus is born in Zhi Ding, April at florescence, spends gorgeous greatly, and first leaf is open, and color is various, and form is graceful, is a kind of ornamental plant having very much Development volue.
The setting percentage of China top Cuculus polioephalus is not low, and every fruit and seed number is at most up to 100；But himself breeding system existing defects, such as Some seeds dysplasia, its germination rate is less than 40%；Seed is without rest period, in the fall after maturation, just can sprout if condition is suitable, but sprout the seedling formed and be difficult to resist boisterous impact in winter, it is easy to dead, survival rate is very low；Most seeds are in next year 3, sprouting in April, and again because seed is little, contained nutrient substance is less, the substantial amounts of nutrient of metabolic consumption of seed self, and germination rate is very low, and it is very thin and delicate to sprout the seedling formed, it is difficult to spending the summer of sweltering heat, survival rate is also very low.Therefore, China's top Cuculus polioephalus quantity is little, only has 330 strains at balcony Mount Huading at present, belongs to minimum population, has been put into Zhejiang Province's rare or endangered species register and has been protected by.
In winding greatly two Natural Populations in Mt. Tiantai, Tiantai County and Panan County, the seedling of China top Cuculus polioephalus is rare, and its reproduction ability is almost lost；The artificial breeding pushing up Cuculus polioephalus simultaneously for China not yet sets up effective method at present.In order to protect rare or endangered species; prevent from being in China's top Cuculus polioephalus extinction of Critical Condition; protective measure must be taked and carry out artificial propagation; utilize artificial breeding method; again breeding successful nursery stock transplanting to suitable environment; to expand the quantity of population, this is significant for protection rare or endangered species.
Summary of the invention
It is an object of the invention to overcome China top Cuculus polioephalus reproduction ability under naturalness weak; manual method also is difficult to breed successful technical problem; the artificial breeding method of a kind of China top Cuculus polioephalus nursery stock is provided; the nursery stock survival rate that China top Cuculus polioephalus breeds is made to improve; to expand the quantity of China top Cuculus polioephalus population; protect this rare or endangered species, and can breed to scale China top Cuculus polioephalus nursery stock.
In order to solve above-mentioned technical problem, the present invention provides the artificial breeding method of a kind of China top Cuculus polioephalus nursery stock, comprises the following steps.
(1) fruit collection: gathering ripe China's top Cuculus polioephalus fruit, acquisition time is annual early November to late November.
(2) Seed storage: fruit natural air drying 4 ~ 6 days at 20～30 DEG C that will adopt, manual strip off capsule takes out seed, is positioned over by the seed taken in sealing container, stores under-10 DEG C～-20 DEG C low temperature, and period of storage is 3 ~ 12 months.
(3) seed germination: select well-developed seed, seed disinfectant solution is sterilized, it is carried out with distilled water again, adopt culture dish double-layer filter paper method, illumination box carries out sprouting test, described Germination Temperature is set to 15 ~ 25 DEG C, and light application time is 3 ~ 12 hours/day, and intensity of illumination is 1000 ~ 2500lx.
(4) first time seedling exercising: the 4th day after seed germination was to the 7th day, by in seedling replanting to nutritious substrate, illumination box carries out seedling exercising, light application time is set to 8 ~ 12 hours/day, and intensity of illumination is set to 2000 ~ 5000lx, and arranging temperature is 15 ~ 25 DEG C, relative humidity is 70 ~ 85%, within 1 to 2 day, watering once, make the water-holding capacity of substrate be maintained at 50 ~ 85%, the cultivation time is 1 ~ 3 month.
(5) second time seedling exercising: after first time seedling exercising, forms the seedling replanting with more than 3 true leaves in the flowerpot arranging cultivation matrix, and 3 ~ 6 strains planted by every basin, seedling exercising again in the controlled environment chamber；Described cultivation matrix proportioning is mountain region humus soil: fine sand: base manure=4～6: 2～4: 1～3, the light application time of described phjytotron is set to 8 ~ 12 hours/day, arranging intensity of illumination is 5000 ~ 10000lx, and temperature is 15 ~ 25 DEG C, and relative humidity is 60 ~ 85%；Within 2 to 3 days, watering once, make the water-holding capacity of substrate be maintained at 30 ~ 60%, the cultivation time is 2 ~ 6 months.
(6) Nursery stock transplanting:, after second time seedling exercising, forming the nursery stock of high 30 ~ 50cm, corresponding natural environment can be transplanted to from phjytotron or carry out potted plant.
As a preferred version of the present invention, in described step (1), described acquisition time is annual mid-November to late November.
As a preferred version of the present invention, in described step (2), described storage temperature is set to-10 DEG C～-20 DEG C, and period of storage is 3 ~ 6 months.
As a preferred version of the present invention, in described step (3), described disinfectant solution is 10% sodium hypochlorite, and described Germination Temperature is 18 ~ 22 DEG C, and light application time is 8 ~ 10 hours/day, and intensity of illumination is 1500 ~ 1800lx.
A preferred version as the present invention, in described step (4), in described illumination box, described light application time is 8 ~ 10 hours/day, and intensity of illumination is 3000 ~ 4000lx, and temperature is 20 ~ 22 DEG C, relative humidity is 75 ~ 80%, the water-holding capacity of substrate is maintained at 70 ~ 80%, and the cultivation time is 2 ~ 3 months, to strengthen the seedling adaptive capacity to environment.
As a preferred version of the present invention, in described step (5), the light application time of described phjytotron is 8 ~ 10 hours/day, and intensity of illumination is 7000 ~ 8000lx, and temperature is 20 ~ 22 DEG C, and relative humidity is 70 ~ 80%；The water-holding capacity of substrate is maintained at 40 ~ 50%, and the cultivation time is 4 ~ 5 months, and nursery stock vitality now is the most prosperous, it is easy to transplant survival.
The beneficial effects of the present invention is: the germination rate of (1) seed is high, wherein stores the seed of 3 ~ 6 months, and its germination rate can more than 40%.
(2) seedling percent is high, the normal seedling sprouted, at the mortality rate in seedling exercising stage first time lower than 10%, at the mortality rate in second time seedling exercising stage lower than 20%.
(3) seedling growth being bred as is good, and beautiful design, is suitable for garden planting and potted plant, and Cuculus polioephalus seedling-wood breeding base is pushed up by the China to form scale.
(4) present invention is conducive to rare or endangered species China is pushed up the protection of Cuculus polioephalus, is also beneficial to this plant is developed as ornamental flower kind and utilized.
Accompanying drawing explanation
Fig. 1 is artificial breeding method's flow chart of a kind of China of present invention top Cuculus polioephalus nursery stock.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is further illustrated.
The artificial breeding method of a kind of China top Cuculus polioephalus nursery stock as shown in Figure 1, comprises the following steps: (1) fruit collection: gathering ripe China's top Cuculus polioephalus fruit, acquisition time is annual early November to late November；The collection that Cuculus polioephalus mature fruit should be in good time is pushed up by China, the fruit that different time gathers, and the development condition of its seed is different, and germination rate also has very big-difference；Finding by studying, under identical mating system, the fruit that annual mid-November to late November gathers, the germination rate of its seed is of a relatively high.
(2) Seed storage: adopting different store method, the germination rate of China's top Cuculus polioephalus seed has very big-difference；After deliberation, suitable store method is by fruit natural air drying, then strip off capsule takes out seed, stores at low temperatures with sealing container；Fruit natural air drying 4 ~ 6 days at 20～30 DEG C such as will adopted, manual strip off capsule takes out seed, is positioned over by the seed taken in sealing container, stores under-10 DEG C～-20 DEG C low temperature, and period of storage is 3 ~ 12 months；Owing to the seed of China top Cuculus polioephalus is only small, contained nutrient substance is less, the metabolism of seed self can consume substantial amounts of nutrient, make seed germination be affected, it is therefore desirable to carry out cryopreservation, find by studying, under identical mating system, arranging storage temperature and be-10 DEG C～-20 DEG C, period of storage is 3 ~ 6 months, and the germination rate of its seed is of a relatively high.Above-mentioned sealing container can use plastic seal pocket double casing, and effect is better.
(3) seed germination: select well-developed seed, generally can select hundred air dry weights seed more than 0.12g, and its intraseminal nutrient substance is more, sprouts better, then seed disinfectant solution is sterilized, then is carried out with distilled water；After deliberation, when other sprouting conditions are identical, when unglazed photograph, light application time deficiency or intensity of illumination are lower than 500lx, the germination rate of its seed all very low (referring to table 1).The present invention adopts culture dish double-layer filter paper method, carries out sprouting test in illumination box, and described Germination Temperature is 15 ~ 25 DEG C, and light application time is 3 ~ 12 hours/day, and intensity of illumination is 1000 ~ 2500lx；As a preferred version of the present invention, described disinfectant solution is 10% sodium hypochlorite, and described Germination Temperature is 18 ~ 22 DEG C, and light application time is 8 ~ 10 hours/day, and intensity of illumination is set to 1500 ~ 1800lx；Adopt said method, start to sprout through about 10 days seeds.
The impact of Cuculus polioephalus seed germination is pushed up in table 1 illumination to China
(4) first time seedling exercising: after seed germination, seedling needs to be transplanted to seedling exercising in nutritious substrate in good time；It is typically in the 4th day after seed germination to the 7th day, by seedling replanting to nutritious substrate, owing to substrate contains abundant nutrient substance, it is possible to meet the growth needs of China top Cuculus polioephalus seedling.After deliberation, when other conditions are identical, the survival rate of seedling is affected very big by cultivation matrix；Such as doing substrate with paddy field soil and fertilizer mixing, seedling development is slow, and mortality rate is high, and the survival rate of seedling is less than 20%；Mixing with high mountain yellow soil and fertilizer and do seedling substrate, after cultivating 1 month, survival rate is less than 50%；Mix with nutrient matrix and fine sand, survival rate more than 90%(referring to table 2).Then carrying out seedling exercising in illumination box, arranging light application time is 8 ~ 12 hours/day, and intensity of illumination is 2500 ~ 5000lx, temperature is 15 ~ 25 DEG C, and relative humidity is 70 ~ 85%, within 1 to 2 day, waters once, the water-holding capacity making substrate is maintained at 50 ~ 85%, and the cultivation time is 1 ~ 3 month；As a preferred version of the present invention, in illumination box, arranging light application time is 8 ~ 10 hours/day, intensity of illumination is 3000 ~ 4000lx, and temperature is 20 ~ 22 DEG C, and relative humidity is 75 ~ 80%, the water-holding capacity of substrate is maintained at 70 ~ 80%, and the cultivation time is 2 ~ 3 months；Pass through seedling exercising, it is possible to strengthen the seedling adaptive capacity to environment.
The different cultivation matrix of table 2 pushes up the impact of Cuculus polioephalus seedling percent to China
(5) second time seedling exercising: need in good time to be transplanted in certain cultivation matrix seedling exercising again through the seedling of first time seedling exercising.It has been investigated that, different times is transplanted and adopts different cultivation matrixes, and seedling percent has very big-difference (referring to table 3).After first time seedling exercising, form the seedling replanting with more than 3 true leaves in the flowerpot that cultivation matrix is set, plantation 3 ~ 6 strains of every basin, seedling exercising again in the controlled environment chamber；Described cultivation matrix proportioning is mountain region humus soil: fine sand: base manure=4～6: 2～4: 1～3, when second time seedling exercising, need to properly increase intensity of illumination and light application time, the light application time of described phjytotron is 8 ~ 12 hours/day, intensity of illumination is 5000 ~ 10000lx, temperature is 15 ~ 25 DEG C, and relative humidity is 60 ~ 85%；Within 2 to 3 days, watering once, make the water-holding capacity of substrate be maintained at 30 ~ 60%, the cultivation time is 2 ~ 6 months；As a preferred version of the present invention, light application time in the controlled environment chamber is set to 10 ~ 11 hours/day, and intensity of illumination is set to 7000 ~ 8000lx, and temperature is set to 20 ~ 22 DEG C, and relative humidity is set to 70 ~ 80%；The water-holding capacity of substrate is maintained at 40 ~ 50%, and the cultivation time is 4 ~ 5 months；By second time seedling exercising, the well-grown of nursery stock, the survival rate of transplanting is high.
The different transplanting time of table 3 and the cultivation matrix impact on seedling percent
(6) Nursery stock transplanting:, after second time seedling exercising, forming the nursery stock of high 30 ~ 50cm, be transplanted to corresponding natural environment from phjytotron or carry out potted plant.
Embodiment 1: in mid-November, plucks China's top Cuculus polioephalus fruit, in the environment of 25 DEG C of aeration-dryings air-dry 5 days, strip off capsule, takes full seed 200, carries out sprouting experiment after preserving 90 days at-20 DEG C, seed with 10% hypochlorite disinfectant 10min, 20 DEG C of distilled water immersions 8 hours；Again seed being put into germination basin, germinate at illumination box, arrange temperature 20 DEG C, intensity of illumination is 1500lx, light application time 8 hour/day；Within 10th day, starting to sprout, within the 17th day, always sprout several 95, germination rate is 48%.By in the seedling subcultivation of sprouting to the flowerpot that bore is 25cm, nutrient matrix in flowerpot: fine sand is 3: 1, carries out first time seedling exercising in illumination box, arranges constant temperature 20 DEG C, humidity is 75%, illumination 2500lx, light application time 12 hour/day, substrate water-holding capacity controls 75%, grow through 10 ~ 15 days rough leafs, grow through 20 ~ 25 days second true leaves, grow through 28 ~ 40 days the 3rd true leaves, the 4th true leaf occurred in the 45th day；To have the strain of 4 ~ 5 true leaf seedling 86 again to be transplanted in cultivation matrix and carry out second time seedling exercising, this cultivation matrix is mountain region humus soil: fine sand: base manure is 5: 2: 1, seedling exercising again in the controlled environment chamber, arranging light application time is 10 hours/day, day temperature is 20 ~ 25 DEG C, nocturnal temperature is 15 ~ 20 DEG C, relative humidity is 75%, intensity of illumination is 8000lx, the water-holding capacity of substrate is 60%, nursery stock 72 strain forming height >=35cm on the 60th day, passes through said method, first time seedling exercising seedling percent is 90.5%, second time seedling exercising seedling percent 83.7%；Again in seedling replanting to corresponding natural environment from phjytotron.
Embodiment 2: in late November, plucks China's top Cuculus polioephalus fruit, in the environment of 20 DEG C of aeration-dryings air-dry 6 days, strip off capsule, takes full seed 200, carries out sprouting experiment after preserving 120 days at-10 DEG C, seed with 10% hypochlorite disinfectant 10min, 20 DEG C of distilled water immersions 10 hours；Again seed being put into germination basin, germinate at illumination box, arrange temperature 15 DEG C, intensity of illumination is 1000lx, light application time 5 hour/day；Within 10th day, starting to sprout, total number of sprouting of the 17th day seed is 87, and germination rate is 43.6%.By in the seedling subcultivation of sprouting to the flowerpot that bore is 25cm, nutrient matrix in flowerpot: fine sand is 2: 1, illumination box carries out first time seedling exercising, arranging temperature is 20 DEG C, humidity is 80%, illumination 3000lx, light application time 10 hour/day, substrate water-holding capacity controls 80%, within 12nd ~ 16 day, rough leaf grows, and within the 20th ~ 25 day, second true leaf grows, and within the 30th ~ 42 day, the 3rd true leaf grows, within 46th day, starting to grow the 4th true leaf, seedling numbers is 79 strains；Again the strain of 4 ~ 5 true leaf seedling 79 is transplanted in cultivation matrix and carries out second time seedling exercising, this cultivation matrix is mountain region humus soil: fine sand: base manure is 5: 3: 2, seedling exercising again in the controlled environment chamber, arranging light application time is 11 hours/day, day temperature is 22 DEG C, nocturnal temperature is 15 DEG C, relative humidity is 80%, intensity of illumination is 9000lx, the water-holding capacity of substrate is 50%, nursery stock 62 strain forming height >=35cm on 80th day, pass through said method, first time seedling exercising seedling percent is 90.8%, second time seedling exercising seedling percent 78.5%, again in seedling replanting to corresponding natural environment from phjytotron.
Embodiment 3: in mid-November, plucks China's top Cuculus polioephalus fruit, in the environment of 25 DEG C of aeration-dryings air-dry 5 days, strip off capsule, takes full seed 200, carries out sprouting experiment after preserving 180 days at-20 DEG C, seed with 10% hypochlorite disinfectant 10min, 20 DEG C of distilled water immersions 8 hours；Again seed being put into germination basin, germinate at illumination box, arrange temperature 25 DEG C, intensity of illumination is 2000lx, and light application time is 3 hours/day；Within 11st day, starting to sprout, total number of sprouting of the 17th day seed is 70, and germination rate is 35%；By in the seedling subcultivation of sprouting to the flowerpot that bore is 25cm, nutrient matrix in flowerpot: fine sand is 3: 1, illumination box carries out first time seedling exercising, arranging temperature is 15 DEG C, humidity is 70%, and intensity of illumination is 2000lx, light application time 8 hour/day, substrate water-holding capacity controls 50%, within 11st ~ 16 day, rough leaf grows, and within the 18th ~ 25 day, second true leaf grows, and within the 28th ~ 40 day, the 3rd true leaf grows, within 45th day, starting to grow the 4th true leaf, the 4th seedling numbers that true leaf grows is 56 strains；Again the strain of 4 ~ 5 true leaf seedling 56 is transplanted in cultivation matrix and carries out second time seedling exercising, this cultivation matrix is mountain region humus soil: fine sand: base manure is 4: 2: 1, seedling exercising again in the controlled environment chamber, arranging light application time is 8 hours/day, day temperature is 25 DEG C, nocturnal temperature is 20 DEG C, relative humidity is 60%, intensity of illumination is 6000lx, the water-holding capacity of substrate is 30%, nursery stock 37 strain forming height >=35cm on 80th day, pass through said method, first time seedling exercising seedling percent is 80%, second time seedling exercising seedling percent 66.0%, again in seedling replanting to corresponding natural environment from phjytotron.
Embodiment 4: in late November, plucks China's top Cuculus polioephalus fruit, in the environment of 20 DEG C of aeration-dryings air-dry 6 days, strip off capsule, takes full seed 200, carries out sprouting experiment after preserving 360 days at-20 DEG C, seed with 10% hypochlorite disinfectant 10min, 20 DEG C of distilled water immersions 10 hours；Again seed being put into germination basin, germinate at illumination box, arrange temperature 25 DEG C, intensity of illumination is 2500lx, light application time 12 hour/day；Within 9th day, starting to sprout, the 17th day always sprouts number is 63, germination rate 31.5%.By in the seedling subcultivation of sprouting to the flowerpot that bore is 25cm, nutrient matrix in flowerpot: fine sand is 2: 1, illumination box carries out first time seedling exercising, constant temperature 25 DEG C is set, humidity is 85%, illumination 5000lx, light application time 12 hour/day, substrate water-holding capacity controls 85%, within 11st ~ 15 day, rough leaf grows, and within the 20th ~ 25 day, second true leaf grows, and within the 28th ~ 40 day, the 3rd true leaf grows, 4th true leaf occurred in 45th day, amount to seedling 45 strain；Seedling 45 strain with 4 ~ 5 true leaves is transplanted in cultivation matrix and carries out second time seedling exercising, this cultivation matrix is mountain region humus soil: fine sand: base manure is 6: 4: 3, seedling exercising again in the controlled environment chamber, arranging light application time is 12 hours/day, day temperature is 20 ~ 25 DEG C, nocturnal temperature is 15 ~ 20 DEG C, relative humidity is 85%, intensity of illumination is 10000lx, the water-holding capacity of substrate is 60%, nursery stock 28 strain forming height >=35cm on the 70th day, passes through said method, first time seedling exercising seedling percent is 71.4%, and second time seedling exercising seedling percent is 62.2%；Again in seedling replanting to corresponding natural environment from phjytotron.
Above in conjunction with accompanying drawing, embodiment of the present invention is explained in detail; but the present invention is not limited to above-mentioned embodiment; to those skilled in the art, it is also possible to make some modification and improvement under the premise of not departing from the present invention, these also should be regarded as belonging to protection scope of the present invention.
Claims (6)
1. an artificial breeding method for China top Cuculus polioephalus nursery stock, comprises the following steps:
(1) fruit collection: gathering ripe China's top Cuculus polioephalus fruit, acquisition time is annual early November to late November；
(2) Seed storage: fruit natural air drying 4 ~ 6 days at 20～30 DEG C that will adopt, manual strip off capsule takes out seed, is positioned over by the seed taken in sealing container, stores under-10 DEG C～-20 DEG C low temperature, and period of storage is 3 ~ 12 months；
(3) seed germination: select well-developed seed, seed disinfectant solution is sterilized, it is carried out with distilled water again, adopt culture dish double-layer filter paper method, illumination box carries out sprouting test, described Germination Temperature is set to 15 ~ 25 DEG C, and light application time is 3 ~ 12 hours/day, and intensity of illumination is set to 1000 ~ 2500lx；
(4) first time seedling exercising: the 4th day after seed germination was to the 7th day, by in seedling replanting to nutritious substrate, illumination box carries out seedling exercising, light application time is set to 8 ~ 12 hours/day, and intensity of illumination is set to 2500 ~ 5000lx, and arranging temperature is 15 ~ 25 DEG C, relative humidity is 70 ~ 85%, within 1 to 2 day, watering once, make the water-holding capacity of substrate be maintained at 50 ~ 85%, the cultivation time is 1 ~ 3 month；
(5) second time seedling exercising: after first time seedling exercising, forms the seedling replanting with more than 3 true leaves in the flowerpot arranging cultivation matrix, and 3 ~ 6 strains planted by every basin, seedling exercising again in the controlled environment chamber；Cultivation matrix proportioning used is mountain region humus soil: fine sand: base manure=4～6: 2～4: 1～3, the light application time of described phjytotron is set to 8 ~ 12 hours/day, arranging intensity of illumination is 5000 ~ 10000lx, and temperature is 15 ~ 25 DEG C, and relative humidity is 60 ~ 85%；Within 2 to 3 days, watering once, make the water-holding capacity of substrate be maintained at 30 ~ 60%, the cultivation time is 2 ~ 6 months；
(6) Nursery stock transplanting:, after second time seedling exercising, forming the nursery stock of high 30 ~ 50cm, corresponding natural environment can be transplanted to from phjytotron or carry out potted plant.
2. the artificial breeding method of a kind of China according to claim 1 top Cuculus polioephalus nursery stock, it is characterised in that: in described step (1), described acquisition time is annual mid-November to late November.
3. the artificial breeding method of a kind of China according to claim 1 top Cuculus polioephalus nursery stock, it is characterised in that: in described step (2), described storage temperature is set to-10 DEG C～-20 DEG C, and period of storage is 3 ~ 6 months.
4. the artificial breeding method of a kind of China according to claim 1 top Cuculus polioephalus nursery stock, it is characterized in that: in described step (3), described disinfectant solution is 10% sodium hypochlorite, and described Germination Temperature is 18 ~ 22 DEG C, light application time is 8 ~ 10 hours/day, and intensity of illumination is 1500 ~ 1800lx.
5. the artificial breeding method of a kind of China according to claim 1 top Cuculus polioephalus nursery stock, it is characterized in that: in described step (4), in described illumination box, described light application time is set to 8 ~ 10 hours/day, intensity of illumination is 3000 ~ 4000lx, and temperature is 20 ~ 22 DEG C, and relative humidity is 75 ~ 80%, the water-holding capacity of substrate is maintained at 70 ~ 80%, and the cultivation time is 2 ~ 3 months.
6. the artificial breeding method of a kind of China according to claim 1 top Cuculus polioephalus nursery stock, it is characterized in that: in described step (5), the light application time of described phjytotron is 8 ~ 10 hours/day, intensity of illumination is 7000 ~ 8000lx, temperature is 20 ~ 22 DEG C, relative humidity is 70 ~ 80%, and the water-holding capacity of substrate is maintained at 40 ~ 50%, and the cultivation time is 3 ~ 5 months.
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| CN114586596A (en) * | 2022-03-15 | 2022-06-07 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | Breeding method of unique ornamental plant rhododendron litchi seeds |
| CN115088576A (en) * | 2022-08-22 | 2022-09-23 | 中国科学院昆明植物研究所 | Low-temperature germination and seedling hardening method for artemisia plants of alpine flowers |
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| CN108513757A (en) * | 2018-04-03 | 2018-09-11 | 安徽农业大学 | Promote the method for cuckoo germination |
| CN110558130A (en) * | 2019-09-23 | 2019-12-13 | 台州学院 | Cutting method of cauliflower |
| CN110558130B (en) * | 2019-09-23 | 2021-06-15 | 台州学院 | Cutting method of cauliflower |
| CN112889393A (en) * | 2021-01-21 | 2021-06-04 | 洛阳师范学院 | Method for artificially breeding seedlings of rare or endangered plant lingbao rhododendron seeds |
| CN114586596A (en) * | 2022-03-15 | 2022-06-07 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | Breeding method of unique ornamental plant rhododendron litchi seeds |
| CN115088576A (en) * | 2022-08-22 | 2022-09-23 | 中国科学院昆明植物研究所 | Low-temperature germination and seedling hardening method for artemisia plants of alpine flowers |
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