CN108513757A - Promote the method for cuckoo germination - Google Patents
Promote the method for cuckoo germination Download PDFInfo
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- CN108513757A CN108513757A CN201810292897.8A CN201810292897A CN108513757A CN 108513757 A CN108513757 A CN 108513757A CN 201810292897 A CN201810292897 A CN 201810292897A CN 108513757 A CN108513757 A CN 108513757A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The present invention relates to a kind of methods promoting cuckoo germination, include the following steps：(1) cuckoo seed is collected；(2) low-temperature treatment：Seed is put into 15 DEG C of refrigerators 3 10 days；(3) seed disinfection：Seed is immersed into alcohol, is rinsed with water, aseptic water washing is used after then being impregnated with mercuric chloride solution, is dried；(4) intermittent warming：Seed is put into 20 30 DEG C of incubator and is handled 47 hours, then moves into refrigerator and handles；(5) seed is impregnated：By in the seed soaking water of intermittent warming, after be immersed in 20 30min in the heteroauxin of 0.3mg/ml 0.5mg/ml or be immersed in 10 30min in the gibberellin of 0.5mg/ml 2.5mg/ml；(6) it sows：The seed impregnated is positioned over after shady place is dried and is broadcasted sowing in matrix, 0.3 0.7cm of matrix cladding thickness.This method can effectively improve germination percentage and speed of germination.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to a method of promoting cuckoo germination.
Background technology
Cuckoo is one of the ten big famous flowers of China, and wherein Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain is that the province in Anhui Province spends, and originates in Mt. Huang in Anhui, refrigerant
The ground such as peak, withered ox drop, Tianzhushan, the ground such as Tianmu Mountains of Zhejiang Province, Jiangxi Huaiyu Shan Mountain and Wugong Shan Mountain are also distributed, and Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain growth is more
On the border of height above sea level 750-1700m, precipice and in mountain valley or thick forest.Have in the North Sea in Mount Huang, Tian Hai sheet of point a large amount of
Cloth.When full blossom in May, all over the mountains and plains, collects white, red, powder, is purple in a tree, it is fragrant gorgeous, it is extremely grand.The month at florescence 5-6, fructescence
The 9-10 months.Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain is particle seed, and seed has wing but wing is undeveloped, and storage nutriment is limited in seed, thousand only heavy
0.2674g.Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain seed has dormant trait, and sowing germination percentage is relatively low, and the germination of seed decides surviving for Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain,
So first to break the dormant trait of seed.Because being not quite similar with other cuckoo growing environments, so Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain seed uses it
The method of his cuckoo germination, such as existing literature are recorded, and Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain is soaked seed using 3% hydrogen peroxide, and 30min (shut out by dewdrop
Cuckoo) afterwards directly sowing germination percentage be only 15%；It is directly sowed using 40% sulfuric acid seed soaking 20min (azalea), germination percentage is only
10%, effect is unsatisfactory.
Therefore, it is necessary to develop a kind of method of promotion cuckoo germination, germination percentage is improved, speed of germination is improved.
Invention content
The purpose of the present invention is to provide a kind of method of promotion cuckoo germination, this method can effectively improve germination percentage, carry
High germination speed.
The technical solution adopted by the present invention is as follows：
A kind of method of cuckoo germination, includes the following steps successively：
(1) cuckoo seed is collected；
(2) low-temperature treatment：Seed is put into 1-5 DEG C of refrigerator 3-10 days；
(3) seed disinfection：Seed is immersed into 10-30s in alcohol, is rinsed with water 3-4 times, then uses 0.05-0.15wt%
Mercuric chloride solution uses aseptic water washing 3-4 times after impregnating 10-30min, dries；
(4) intermittent warming：Seed after disinfection is subjected to intermittent warming, specific method is：Seed is put into 20-30 DEG C
It is handled 4-7 hours in incubator, then moves into 1-5 DEG C of refrigerator and handle 4-7 hours；Intermittent warming keeps seed kind skin flexible
Change and cause kind of a skin rupture, enhances Seed imbibition ability, to increase the germination percentage of seed；
(5) seed is impregnated：By 20-30h in the seed soaking water of intermittent warming, after be immersed in 0.3mg/ml-0.5mg/ml
IAA (heteroauxin) in 20-30min or be immersed in the GA of 0.5mg/ml-2.5mg/ml310-30min in (gibberellin)；
Hormone concentration is too low, and germination percentage and speed of germination cannot improve, and concentration height can inhibit the germination of seed, excessive concentration, seed
It will never germinate.
(6) it sows：The seed impregnated is positioned over after 15 DEG C of -18 DEG C of shady places are dried and is broadcasted sowing in matrix, base is used in combination
Matter covers, cladding thickness 0.3-0.7cm, and environment temperature is 20-25 DEG C, can spray water appropriate and keep the moistening of matrix, it is empty
Air humidity degree is 70-75%.
It chooses that growth conditions are excellent and the Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of no disease and pests harm when wherein step (1) collects seed, it is full to collect seed
Full mature seed is sealed after 15 DEG C of -18 DEG C of shady places are dried；
Wherein step (2) low-temperature treatment when preferably the seed dried is put into 4 DEG C of refrigerators 4-6 days；
Wherein step (3) seed disinfection when seed immersed into 20s in 70% alcohol, with aseptic water washing 3-4 times, then use
0.1wt% mercuric chloride solutions use aseptic water washing 3-4 times after impregnating 20min, are positioned on shady place aseptic filter paper and dry；
The temperature of wherein step (4) incubator is excessively high, and seed can be inactivated because losing more juicy, and germination percentage reduces, temperature
It spends low, so that seed is entered deeper rest period, handle 6 hours in preferably 25 DEG C of incubator, then move into the refrigerator to 4 DEG C
In handle 6 hours, refrigerator processing play the role of promote germination, be similar to vernalization.This is a cycle, is operated repeatedly
4-5 period.
Wherein the concentration of heteroauxin is preferably 0.5mg/ml in step (5), and the concentration of gibberellin is preferably 1.5mg/ml.
Wherein the seed impregnated is positioned over by step (6) dried on 15 DEG C of -18 DEG C of shady place aseptic filter papers after broadcast sowing in
In the matrix of fertile soil and vermiculite, the weight ratio of fertile soil and vermiculite is 1: (1-2), matrix use 120 DEG C of sterilizing 20-30min,
It is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm.
Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain germination of the present invention has the beneficial effect that：
Seed disinfection in step (2) reduces the virus carried on Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain seed, reduces growth wherein Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain
Viral infection in growth period, step (4) can promote seed breaking dormancy, increase the effect of the gas permeability and absorbability of kind of skin,
Step (5) may advantageously facilitate seed moisture content absorption, increase imbibition rate, improve germination percentage.The present invention is using intermittent warming and growth
The form of element processing, the effective time for shortening cuckoo germination and the germination percentage for improving seed.Because of Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain kind
The imbibition ability of sub kind of skin is weaker, is only immersed in hormone, and because of the containment of kind of skin, germination percentage will not be greatly improved seed,
So first breaking the containment of seed kind skin, along with HORMONE TREATMENT can improve germination percentage, speed of germination is improved.
Specific implementation mode
Embodiment of the present invention is described in detail below in conjunction with example, but those skilled in the art will manage
Solution, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.
Following embodiment, comparative example sowing condition are 20-25 DEG C of environment temperature, keep the moistening of matrix, air humidity to be
70-75%.It wherein sows after the seed treatment of embodiment 2-3 and comparative example, tests simultaneously simultaneously.
Embodiment 1
(1) seed is collected：By the end of October, it chooses that growth conditions are excellent and the Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of no disease and pests harm, collects full seed
Mature seed, packed after 15 DEG C of -18 DEG C of shady places are dried；
(2) low-temperature treatment：The dry seed of drying is put into 4 DEG C of refrigerators and is handled 5 days；
(3) seed disinfection：Seed is immersed into 20s in 70% alcohol, with aseptic water washing 3 times, then with 0.1% mercury chloride
Aseptic water washing is used after aqueous solution soaking 20min 3 times, be positioned on shady place aseptic filter paper and dry；
(4) intermittent warming：Seed after low-temperature treatment is subjected to intermittent warming, specific method is：Seed is put into 25 DEG C
Incubator in handle 6 hours, handled 6 hours in then moving into 4 DEG C of refrigerator, this is a cycle, repeatedly operate 5 week
Phase.
(5) seed is impregnated：At room temperature, it will be immersed in pure water for 24 hours by above step seed, then be separately immersed in
IAA (heteroauxin) a concentration of 0.3mg/ml, 0.4mg/ml, 0.5mg/ml, time 10min, 20min, 30min, Huo Zhenong
Degree is the GA of 0.5mg/m1,1.5mg/ml, 2.5mg/ml3(gibberellin), time 10min, 20min, 30min；
(6) it sows：The seed impregnated is positioned over after being dried on shady place aseptic filter paper and is broadcasted sowing in fertile soil: vermiculite=
In 1: 1 matrix, matrix is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm using 120 DEG C of sterilizing 20min.
Germinating status such as table 1, table 2 under Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain seed different disposal：
Germination percentage of 1 Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of table under different disposal
Germinating time of 2 Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of table after different disposal
It germinates within 15 days by IAA (heteroauxin) the processing 30min Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlains seed of 0.5mg/ml, germination percentage is
The GA of 88% or 1.5mg/ml3It handles Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain to germinate for 17 days, germination percentage 72%, and has extremely after rarely having seed sprouting
The case where dying.It can be seen from above example by the immersion of hormone can make up in seed store nutriment it is limited lack
It falls into, germination percentage can be effectively improved, improve speed of germination.The relatively thin seed of other kind of skin can directly use hormone seed soaking, because
Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain seed kind skin imbibition is smaller, and the rupture of seed kind skin need to be made to be jointly processed by with hormone, germination percentage is can effectively improve, carry
High germination speed.
Embodiment 2
(1) seed is collected：By the end of October, it chooses that growth conditions are excellent and the Mount Emei goldentop cuckoo of no disease and pests harm, collects seed
The full mature seed of grain, packs after 15 DEG C of -18 DEG C of shady places are dried；
(2) low-temperature treatment：The dry seed of drying is put into 4 DEG C of refrigerators and is handled 5 days；
(3) seed disinfection：Seed is immersed into 20s in 70% alcohol, with aseptic water washing 3 times, then with 0.1% mercury chloride
Aseptic water washing is used after aqueous solution soaking 20min 3 times, be positioned on shady place aseptic filter paper and dry；
(4) intermittent warming：Seed after low-temperature treatment is subjected to intermittent warming, specific method is：Seed is put into 25 DEG C
Incubator in handle 6 hours, handled 6 hours in then moving into 4 DEG C of refrigerator, this is a cycle, repeatedly operate 5 week
Phase；
(5) seed is impregnated：At room temperature, it will be immersed in pure water for 24 hours by above step seed, and then be immersed in IAA (Yin
Indolylbutyric acid) a concentration of 0.5mg/m1, time 30min；
(6) it sows：The seed impregnated is positioned over after being dried on shady place aseptic filter paper and is broadcasted sowing in fertile soil: vermiculite=
In 1: 1 matrix, matrix is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm using 120 DEG C of sterilizing 20min.
Treated, and cuckoo seed germinates for 18 days, and germination percentage 60%, no seed has dead situation after sprouting.
Embodiment 3
(1) seed is collected：By the end of October, it chooses that growth conditions are excellent and the Mount Emei goldentop cuckoo of no disease and pests harm, collects seed
The full mature seed of grain, packs after 15 DEG C of -18 DEG C of shady places are dried；
(2) low-temperature treatment：The dry seed of drying is put into 4 DEG C of refrigerators and is handled 5 days；
(3) seed disinfection：Seed is immersed into 20s in 70% alcohol, with aseptic water washing 3 times, then with 0.1% mercury chloride
Aseptic water washing is used after aqueous solution soaking 20min 3 times, be positioned on shady place aseptic filter paper and dry；
(4) intermittent warming：Seed after low-temperature treatment is subjected to intermittent warming, specific method is：Seed is put into 25 DEG C
Incubator in handle 6 hours, handled 6 hours in then moving into 4 DEG C of refrigerator, this is a cycle, repeatedly operate 5 week
Phase；
(5) seed is impregnated：At room temperature, it will be immersed in pure water for 24 hours by above step seed, and then be immersed in 1.5mg/
The GA of m13In, time 30min；
(6) it sows：The seed impregnated is positioned over after being dried on shady place aseptic filter paper and is broadcasted sowing in fertile soil: vermiculite=
In 1: 1 matrix, matrix is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm using 120 DEG C of sterilizing 20min.
Treated, and cuckoo seed germinates for 19 days, germination percentage 55%, and has dead situation after rarely having seed sprouting.
Comparative example 1
(1) seed is collected：By the end of October, it chooses that growth conditions are excellent and the Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of no disease and pests harm, collects full seed
Mature seed, 15 DEG C of -18 DEG C of shady places pack after drying；
(2) seed is impregnated：At room temperature, by the seed dried impregnate respectively in pure water for 24 hours 10min, for 24 hours 20min,
24h30min。
(3) it sows：The seed impregnated is positioned over after being dried on shady place aseptic filter paper and is broadcasted sowing in fertile soil: vermiculite=
In 1: 1 matrix, matrix is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm using 120 DEG C of sterilizing 20min.By this
The germination of Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain seed 22 days (soaking time for 24 hours 20min) of case method processing, germination percentage 35%, and Some seeds
Have after sprouting because there is a phenomenon where death for the reasons such as the different moulds of infection.
Comparative example 2
(1) seed is collected：By the end of October, it chooses that growth conditions are excellent and the Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of no disease and pests harm, collects full seed
Mature seed, packed after 15 DEG C of -18 DEG C of shady places are dried；
(2) low-temperature treatment：The dry seed of drying is put into 4 DEG C of refrigerators and is handled 5 days；
(3) seed disinfection：Seed is immersed into 20s in 70% alcohol, with aseptic water washing 3 times, then with 0.1% mercury chloride
Aseptic water washing is used after aqueous solution soaking 20min 3 times, be positioned on shady place aseptic filter paper and dry；
(4) intermittent warming：Seed after low-temperature treatment is subjected to intermittent warming, specific method is：Seed is put into 40 DEG C
Incubator in handle 6 hours, handled 6 hours in then moving into 4 DEG C of refrigerator, this is a cycle, repeatedly operate 5 week
Phase；
(5) seed is impregnated：At room temperature, it will be immersed in pure water for 24 hours by above step seed, and then be immersed in IAA (Yin
Indolylbutyric acid) a concentration of 0.5mg/ml, time 30min；
(6) it sows：The seed impregnated is positioned over after being dried on shady place aseptic filter paper and is broadcasted sowing in fertile soil: vermiculite=
In 1: 1 matrix, matrix is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm using 120 DEG C of sterilizing 20min.
Treated, and Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain germinates for 20 days, and the temperature of germination percentage 30%, the comparative example incubator is excessively high, seed
Kind skin rupture is more thorough, but because therefore the excessively high embryo of temperature can also lose more moisture, germination percentage can substantially reduce.
Comparative example 3
(1) seed is collected：By the end of October, it chooses that growth conditions are excellent and the Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of no disease and pests harm, collects full seed
Mature seed, packed after 15 DEG C of -18 DEG C of shady places are dried；
(2) low-temperature treatment：The dry seed of drying is put into 4 DEG C of refrigerators and is handled 5 days；
(3) seed disinfection：Seed is immersed into 20s in 70% alcohol, with aseptic water washing 3 times, then with 0.1% mercury chloride
Aseptic water washing is used after aqueous solution soaking 20min 3 times, be positioned on shady place aseptic filter paper and dry；
(4) intermittent warming：Seed after low-temperature treatment is subjected to intermittent warming, specific method is：Seed is put into 15 DEG C
Incubator in handle 6 hours, handled 6 hours in then moving into 4 DEG C of refrigerator, this is a cycle, repeatedly operate 5 week
Phase.
(5) seed is impregnated：At room temperature, it will be immersed in pure water for 24 hours by above step seed, and then be immersed in IAA (Yin
Indolylbutyric acid) a concentration of 0.5mg/ml, time 30min；
(6) it sows：The seed impregnated is positioned over after being dried on shady place aseptic filter paper and is broadcasted sowing in fertile soil: vermiculite=
In 1: 1 matrix, matrix is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm using 120 DEG C of sterilizing 20min.
Treated, and Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain germinates for 19 days, and germination percentage 46%, the comparative example incubator temperature is too low, cannot make kind
The kind skin of son smoothly ruptures, while seed being made to enter deeper rest period, and germination percentage reduces.
Comparative example 4
(1) seed is collected：By the end of October, it chooses that growth conditions are excellent and the Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of no disease and pests harm, collects full seed
Mature seed, packed after 15 DEG C of -18 DEG C of shady places are dried；
(2) low-temperature treatment：The dry seed of drying is put in refrigerator and is handled 5 days；
(3) seed disinfection：Seed is immersed into 20s in 70% alcohol, with aseptic water washing 3 times, then with 0.1% mercury chloride
Aseptic water washing is used after aqueous solution soaking 20min 3 times, be positioned on shady place aseptic filter paper and dry；
(4) intermittent warming：Seed after low-temperature treatment is subjected to intermittent warming, specific method is：In water temperature gradually by 55
It DEG C is down in 35 DEG C of warm water and to impregnate 12 hours, this is a cycle, operates 5 periods repeatedly.
(5) seed is impregnated：At room temperature, it will be immersed in pure water for 24 hours by above step seed, and then be immersed in IAA (Yin
Indolylbutyric acid) a concentration of 0.5mg/ml, time 30min；
(6) it sows：The seed impregnated is positioned over after being dried on shady place aseptic filter paper and is broadcasted sowing in fertile soil: vermiculite=
In 1: 1 matrix, matrix is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm using 120 DEG C of sterilizing 20min.
Treated, and Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain germinates for 19 days, germination percentage 46%, which is gradual change, alternating temperature
Relatively slowly, and temperature is excessively high, and the kind skin of seed cannot be made smoothly to rupture, and germination percentage reduces, and the cataclysm that the present invention is temperature, this
Sample kind skin more completely ruptures.
Comparative example 5
(1) seed is collected：By the end of October, it chooses that growth conditions are excellent and the Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of no disease and pests harm, collects full seed
Mature seed, packed after 15 DEG C of -18 DEG C of shady places are dried；
(2) low-temperature treatment：The dry seed of drying is put into 4 DEG C of refrigerators and is handled 5 days；
(3) seed disinfection：Seed is immersed into 20s in 70% alcohol, with aseptic water washing 3 times, then with 0.1% mercury chloride
Aseptic water washing is used after aqueous solution soaking 20min 3 times, be positioned on shady place aseptic filter paper and dry；
(4) intermittent warming：Seed after low-temperature treatment is subjected to intermittent warming, specific method is：Seed is put into 25 DEG C
Incubator in handle 6 hours, handled 6 hours in then moving into 4 DEG C of refrigerator, this is a cycle, repeatedly operate 5 week
Phase.
(5) seed is impregnated：At room temperature, it will be immersed in pure water for 24 hours by above step seed, and then be immersed in IAA (Yin
Indolylbutyric acid) a concentration of 2mg/m1, time 30min；
(6) it sows：The seed impregnated is positioned over after being dried on shady place aseptic filter paper and is broadcasted sowing in fertile soil: vermiculite=
In 1: 1 matrix, matrix is used in combination above-mentioned mixed-matrix to cover, cladding thickness 0.5cm using 120 DEG C of sterilizing 20min.
Treated, and Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain germinates for 20 days, germination percentage 30%, comparative example step (5) the IAA excessive concentrations, germination
Rate reduces.Concentration height can inhibit the germination of seed, excessive concentration, seed that will never germinate.
Claims (9)
1. a kind of method promoting cuckoo germination, it is characterised in that in turn include the following steps：
(1) cuckoo seed is collected；
(2) low-temperature treatment：Seed is put into 1-5 DEG C of refrigerator 3-10 days；
(3) seed disinfection：Seed is immersed into 10-30s in alcohol, is rinsed with water 3-4 times, 0.05-0.15wt% chlorinations are then used
Mercury aqueous solution soaking 10-30min, uses aseptic water washing 3-4 times afterwards, dries；
(4) intermittent warming：Specific method is：Seed is put into 20-30 DEG C of incubator and is handled 4-7 hours, then moved into
It is handled 4-7 hours in 1-5 DEG C of refrigerator；
(5) seed is impregnated：By 20-30h in the seed soaking water of intermittent warming, after be immersed in the Yin of 0.3mg/ml-0.5mg/ml
20-30min or it is immersed in 10-30min in the gibberellin of 0.5mg/ml-2.5mg/ml in indolylbutyric acid；
(6) it sows：The seed impregnated is positioned over after 15 DEG C of -18 DEG C of shady places are dried and is broadcasted sowing in matrix, matrix is used in combination to cover
Lid, cladding thickness 0.3-0.7cm.
2. promoting the method for cuckoo germination according to claim 1, it is characterised in that the step (1) is chosen when collecting seed
Growth conditions are excellent and the Rhododendron maculiferum Franchi subsp. Anhweiense (wils) Chamberlain of no disease and pests harm, collect the mature seed of full seed, are dried in 15 DEG C of -18 DEG C of shady places
After be sealed.
3. promoting the method for cuckoo germination according to claim 1, it is characterised in that will dry in the air when step (2) low-temperature treatment
Dry seed is put into 4 DEG C of refrigerators 4-6 days.
4. promoting the method for cuckoo germination according to claim 1, it is characterised in that seed is put into 25 DEG C by the step (4)
Incubator in handle 6 hours, handle 6 hours in then moving into 4 DEG C of refrigerator, operate 4-5 period repeatedly.
5. promoting the method for cuckoo germination according to claim 1, it is characterised in that heteroauxin is dense in the step (5)
Degree is 0.5mg/ml.
6. promoting the method for cuckoo germination according to claim 1, it is characterised in that the concentration of gibberellin in the step (5)
For 1.5mg/ml.
7. promoting the method for cuckoo germination according to claim 1, it is characterised in that the seed that the step (6) will be impregnated
It is positioned over after being dried on 15 DEG C of -18 DEG C of shady place aseptic filter papers and broadcasts sowing in the matrix of fertile soil and vermiculite, fertile soil and vermiculite
Weight ratio be 1: (1-2), matrix using 120 DEG C sterilizing 20-30min, be used in combination mixed-matrix to cover, cladding thickness 0.5cm.
8. promoting the method for cuckoo germination according to claim 7, it is characterised in that step (6) the matrix fertile soil and leech
The weight ratio of stone is 1: 1.
9. promoting the method for cuckoo germination according to claim 1, it is characterised in that environment temperature is when step (6) is sowed
20-25 DEG C, keep the moistening of matrix, air humidity 70-75%.
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| CN201810292897.8A CN108513757A (en) | 2018-04-03 | 2018-04-03 | Promote the method for cuckoo germination |
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Cited By (3)
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| CN112616357A (en) * | 2020-12-18 | 2021-04-09 | 武汉双螺旋生物科技有限公司 | Abnormal temperature germination accelerating technology for seeds of rhododendron anthopogonoides |
| CN112889393A (en) * | 2021-01-21 | 2021-06-04 | 洛阳师范学院 | Method for artificially breeding seedlings of rare or endangered plant lingbao rhododendron seeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109496655A (en) * | 2018-12-03 | 2019-03-22 | 广东省林业科学研究院 | The seed of camellia azalea is sprouted and method for culturing seedlings |
| CN112616357A (en) * | 2020-12-18 | 2021-04-09 | 武汉双螺旋生物科技有限公司 | Abnormal temperature germination accelerating technology for seeds of rhododendron anthopogonoides |
| CN112889393A (en) * | 2021-01-21 | 2021-06-04 | 洛阳师范学院 | Method for artificially breeding seedlings of rare or endangered plant lingbao rhododendron seeds |
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