CN105613238A - Seedling hardening method for sequoia sempervirens - Google Patents
Seedling hardening method for sequoia sempervirens Download PDFInfo
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- CN105613238A CN105613238A CN201511001014.6A CN201511001014A CN105613238A CN 105613238 A CN105613238 A CN 105613238A CN 201511001014 A CN201511001014 A CN 201511001014A CN 105613238 A CN105613238 A CN 105613238A
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- hardening
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Developmental Biology & Embryology (AREA)
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- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention relates to a seedling hardening method for sequoia sempervirens. The method includes the steps that firstly, turf and perlite are mixed at the mass ratio of 1:2-1:1 and then added with a carbendazim solution to prepare a seedling hardening medium, the medium is put into bottles, and the bottles are sterilized for 1-2 h at 120-125 DEG C in an autoclave; then, tissue culture seedlings are transplanted into the sterilized tissue culture bottles and hardened under natural light, wherein 4-6 tissue culture seedlings are put in each tissue culture bottle; finally, pot planting is carried out after the tissue culture seedlings send forth new root systems, and field management is carried out 2 months later, wherein the survival rate of the hardened seedlings reaches 85% or above. The preparation method is simple, and the steps are easy to implement; compared with a traditional seedling hardening method that seedlings are transplanted out of bottles directly, the tissue culture seedlings are hardened at the earlier stage in the bottles, the tissue culture seedlings can adapt to a soil medium under the sterile condition, the root systems suitable for the soil medium are induced, and the survival rate of sequoia sempervirens is greatly increased.
Description
Technical field
The present invention relates to the hardening off method of a kind of sequoia sempervirens, belong to biological culture technology field.
Background technology
Sequoia sempervirens originates in California, United States state seashore, is evergreen needle megaphanerophyte. Trunk is straight and upright, bark sorrel, lobe, and tree crown cone, bough is open and flat. The height of tree 100 meters, the diameter of a cross-section of a tree trunk 1.3 meters above the ground 10 meters is the big tree of the first in the world. Sequoia sempervirens tree appearance is grand, the close life of branches and leaves, and growth is rapidly. Being applicable in lakeside, waterside, lawn isolated planting or mass-planting, view is beautiful, it is possible to the avenue system on both sides of the road along garden, and grandeur is very excellent Landscape Tree Species. But owing to sequoia sempervirens cuttage survival rate is low, tissue culture method is taken root and difficult, and after going out garden, surviving rate is extremely low, often within 1 ~ 2 week, will die, also the hardening domestication of these seeds is not done concrete research by R&D institution and enterprise, and therefore this tree kind is also few in the quantity of China.
Summary of the invention
It is an object of the invention to the problem directly tissue cultured seedling being placed in booth and emerging to solve the lower and traditional hardening off method of sequoia sempervirens cuttage survival rate and cultivate, it provides one method is simple, the hardening off method of the sequoia sempervirens that hardening surviving rate is higher.
The present invention adopts following technical scheme: the hardening off method of a kind of sequoia sempervirens, comprises the steps:
(1) proportioning of hardening matrix: being that 1 ~ 2:1 ~ 3:1 ~ 3 mix in mass ratio by fertile to coconut palm chaff, mushroom, vermiculite, and be evenly mixed with hardening matrix with the carbendazim solution stirring of 800 ~ 1000 times, the humid control of hardening matrix is 40 ~ 60%;
(2) matrix bottling: the hardening matrix being stirred is dispensed in tissue culture bottle, and dispensed loading amount reaches the 30 ~ 40% of bottle body volume, with ventilating cover or ventilative sealing film phonograph seal;
(3) sterilizing of hardening matrix: tissue culture bottle is placed in high-pressure sterilizing pot, sterilizing 1 ~ 2 hour under 120 ~ 125 DEG C of conditions;
(4) tissue cultured seedling change bottle: tissue cultured seedling is transplanted in the tissue culture bottle after sterilizing, puts 4 ~ 6 for every bottle, sealing, be placed on natural light lower refining seedling 15 ~ 30 days, temperature is 20 ~ 25 DEG C;
(5) field planting of tissue cultured seedling: treat that the leaf color of tissue cultured seedling transfers deep green to, base minister goes out 3 ~ 5 new root systems can start field planting, tissue cultured seedling in bottle is carried out potted plant, water permeable with 800 ~ 1000 times of carbendazim solutions, atmospheric moisture keeps 60 ~ 80%, temperature is 10 ~ 20 DEG C, carries out normal field management after 2 months.
Further, the consumption of described carbendazim solution is the 30 ~ 50% of grass carbon and perlite cumulative volume.
Further, in described step (4), first tissue cultured seedling washes whole agar before transplanting in 35 ~ 40 DEG C of warm water, then soaks 5 ~ 10 minutes in the NAA solution of 0.15 ~ 0.2mg/L.
Preparation method of the present invention is simple, step is easy to operation, compared with the hardening off method of bottle outlet direct with tradition, tissue cultured seedling is carried out the strong sprout in early stage by the present invention in bottle, under sterile conditions, tissue cultured seedling is allowed to adapt to soil matrix, induce the root system of applicable soil matrix, tissue cultured seedling is very high to the humidity requirement of air for early stage, it is difficult to reach 90% in usual booth, and cause thirsting, the present invention can maintain higher atmospheric moisture in tissue culture bottle, until new root system grows, after going out garden, new root water uptake is strong, improve the surviving rate of North America China fir greatly, surviving rate is more than 85%.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated.
Comparative example:
(1) the tissue culture bottle lid after taking root is opened, keep atmospheric moisture 80%;
(2) matrix is stirred: by the peat composed of rotten mosses and perlite mixing for 1:2 in mass ratio, and stir preparation with the carbendazim solution of 900 times and become hardening matrix, carbendazim solution amount ranges is the 40% of matrix volume, according to the water content adjustment humidity in former matrix, by the humid control of hardening matrix 50%, do not overflow water as degree to hold matrix;
After (3) 7 days, after bacterium long on substratum, being cleaned by tissue cultured seedling clean, be transplanted in step (2) in the matrix being stirred, keep atmospheric moisture 80%, temperature is at 20 DEG C, and after one month, surviving rate is 11.3%.
Embodiment one:
(1) proportioning of hardening matrix: by coconut palm chaff, mushroom fertilizer, vermiculite in mass ratio for 1:1:1 mixes, and stir preparation with the carbendazim solution of 1000 times and become hardening matrix, carbendazim solution amount ranges is the 30% of matrix volume, according in former matrix water content adjustment humidity, by the humid control of hardening matrix 40% taking hold matrix do not overflow water as degree;
(2) matrix bottling: the matrix being stirred is dispensed in tissue culture bottle, and dispensed loading amount reaches the 30% of bottle body volume, with ventilating cover or ventilative sealing film phonograph seal;
(3) sterilizing of hardening matrix: tissue culture bottle is placed in high-pressure sterilizing pot, sterilizing 2 hours under 120 DEG C of conditions;
(4) tissue cultured seedling change bottle: first tissue cultured seedling is washed in 35 DEG C of warm water whole agar, then soaks in the tissue culture bottle after being transplanted to sterilizing after 10 minutes in the NAA solution of 0.15mg/L, put 4 for every bottle, sealing, being placed on natural light lower refining seedling 15 days, temperature 20 DEG C, humidity is not required;
(5) field planting of tissue cultured seedling: treat that the leaf color of tissue cultured seedling transfers deep green to, base minister goes out 3 new root systems can start field planting, tissue cultured seedling in bottle is carried out potted plant on, water permeable with 800 times of carbendazim solutions, atmospheric moisture remains on 60%, temperature control is made as 10 DEG C, can carry out normal field management, survival rate 88.3% after 2 months.
Embodiment two:
(1) proportioning of hardening matrix: just coconut palm chaff, mushroom be fertile, vermiculite is in mass ratio for 1:1:2 mixes, and stir preparation with the carbendazim solution of 800 times and become hardening matrix, carbendazim solution amount ranges is the 50% of matrix volume, according to the water content adjustment humidity in former matrix, by the humid control of hardening matrix 60%, do not overflow water as degree to hold matrix;
(2) matrix bottling: the matrix being stirred is dispensed in tissue culture bottle, and dispensed loading amount reaches the 40% of bottle body volume, with ventilating cover or ventilative sealing film phonograph seal;
(3) sterilizing of hardening matrix: tissue culture bottle is placed in high-pressure sterilizing pot, sterilizing 1 hour under 121 DEG C of conditions;
(4) tissue cultured seedling change bottle: first tissue cultured seedling is washed in 40 DEG C of warm water whole agar, then soaks in the tissue culture bottle after being transplanted to sterilizing after 5 minutes in the NAA solution of 0.2mg/L, put 6 for every bottle, sealing, being placed on natural light lower refining seedling 30 days, temperature 25 DEG C, humidity is not required;
(5) field planting of tissue cultured seedling: treat that the leaf color of tissue cultured seedling transfers deep green to, base minister goes out 5 new root systems can start field planting, tissue cultured seedling in bottle is carried out potted plant on, water permeable with the carbendazim solution of 1000 times, atmospheric moisture remains on 80%, temperature control is made as 20 DEG C, can carry out normal field management, survival rate 91.2% after 2 months.
Embodiment three:
(1) proportioning of hardening matrix: just coconut palm chaff, mushroom be fertile, vermiculite is in mass ratio for 2:3:3 mixes, and stir preparation with the carbendazim solution of 900 times and become hardening matrix, carbendazim solution amount ranges is the 40% of matrix volume, according to the water content adjustment humidity in former matrix, by the humid control of hardening matrix 50%, do not overflow water as degree to hold matrix;
(2) matrix bottling: the matrix being stirred is dispensed in tissue culture bottle, and dispensed loading amount reaches the 35% of bottle body volume, with ventilating cover or ventilative sealing film phonograph seal;
(3) sterilizing of hardening matrix: tissue culture bottle is placed in high-pressure sterilizing pot, sterilizing 1 hour under 125 DEG C of conditions;
(4) tissue cultured seedling change bottle: first tissue cultured seedling is washed in 35 DEG C of warm water whole agar, then soaks in the tissue culture bottle after being transplanted to sterilizing after 5 minutes in the NAA solution of 0.2mg/L, put 5 for every bottle, sealing, being placed on natural light lower refining seedling 25 days, temperature 22 DEG C, humidity is not required;
(5) field planting of tissue cultured seedling: treat that the leaf color of tissue cultured seedling transfers deep green to, base minister goes out 4 new root systems can start field planting, tissue cultured seedling in bottle is carried out potted plant on, water permeable with 900 times of carbendazim solutions, atmospheric moisture remains on 70%, temperature control is made as 15 DEG C, can carry out normal field management, survival rate 90.6% after 2 months.
Claims (3)
1. the hardening off method of a sequoia sempervirens, it is characterised in that: comprise the steps:
(1) proportioning of hardening matrix: being that 1 ~ 2:1 ~ 3:1 ~ 3 mix in mass ratio by fertile to coconut palm chaff, mushroom, vermiculite, and be evenly mixed with hardening matrix with the carbendazim solution stirring of 800 ~ 1000 times, the humid control of hardening matrix is 40 ~ 60%;
(2) matrix bottling: the hardening matrix being stirred is dispensed in tissue culture bottle, and dispensed loading amount reaches the 30 ~ 40% of bottle body volume, with ventilating cover or ventilative sealing film phonograph seal;
(3) sterilizing of hardening matrix: tissue culture bottle is placed in high-pressure sterilizing pot, sterilizing 1 ~ 2 hour under 120 ~ 125 DEG C of conditions;
(4) tissue cultured seedling change bottle: tissue cultured seedling is transplanted in the tissue culture bottle after sterilizing, puts 4 ~ 6 for every bottle, sealing, be placed on natural light lower refining seedling 15 ~ 30 days, temperature is 20 ~ 25 DEG C;
(5) field planting of tissue cultured seedling: treat that the leaf color of tissue cultured seedling transfers deep green to, base minister goes out 3 ~ 5 new root systems can start field planting, tissue cultured seedling in bottle is carried out potted plant, water permeable with 800 ~ 1000 times of carbendazim solutions, atmospheric moisture keeps 60 ~ 80%, temperature is 10 ~ 20 DEG C, carries out normal field management after 2 months.
2. the hardening off method of sequoia sempervirens as claimed in claim 1, it is characterised in that: the consumption of described carbendazim solution is the 30 ~ 50% of grass carbon and perlite cumulative volume.
3. the hardening off method of sequoia sempervirens as claimed in claim 1, it is characterised in that: in described step (4), first tissue cultured seedling washes whole agar before transplanting in 35 ~ 40 DEG C of warm water, then soaks 5 ~ 10 minutes in the NAA solution of 0.15 ~ 0.2mg/L.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301901A (en) * | 2011-07-29 | 2012-01-04 | 云南省农业科学院花卉研究所 | Sequoia sempervirens seed seedling culture method |
| CN103798143A (en) * | 2014-02-25 | 2014-05-21 | 云南省农业科学院花卉研究所 | Out-bottle cutting and rooting method for sequoia sempervirens tissue culture seedling |
| CN103814735A (en) * | 2014-03-07 | 2014-05-28 | 李明鹤 | America redwood nursery stock large-scale breeding method by building cutting orchard |
| CN104839024A (en) * | 2015-05-18 | 2015-08-19 | 凤阳金小岗农林科技产业发展有限公司 | Tissue culture seedling-exercising method |
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- 2015-12-29 CN CN201511001014.6A patent/CN105613238B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301901A (en) * | 2011-07-29 | 2012-01-04 | 云南省农业科学院花卉研究所 | Sequoia sempervirens seed seedling culture method |
| CN103798143A (en) * | 2014-02-25 | 2014-05-21 | 云南省农业科学院花卉研究所 | Out-bottle cutting and rooting method for sequoia sempervirens tissue culture seedling |
| CN103814735A (en) * | 2014-03-07 | 2014-05-28 | 李明鹤 | America redwood nursery stock large-scale breeding method by building cutting orchard |
| CN104839024A (en) * | 2015-05-18 | 2015-08-19 | 凤阳金小岗农林科技产业发展有限公司 | Tissue culture seedling-exercising method |
Non-Patent Citations (2)
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| 彭绿春等: "《北美红杉嫩枝的标准化离体繁殖技术研究》", 《中国农学通报》 * |
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Address after: 214135 Jiangsu province Wuxi city Wuxi District Taihu international science and Technology Park of University Science and Technology Park Qingyuan Road Industrial Building A, No. 518 Applicant after: Meishang Ecological Landscape Co., Ltd. Address before: 214135 Jiangsu province Wuxi city Wuxi District Taihu international science and Technology Park of University Science and Technology Park Qingyuan Road Industrial Building A, No. 518 Applicant before: Jiangsu Meishang Ecology Landscape Co., Ltd. |
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