CN104839024A - Tissue culture seedling-exercising method - Google Patents
Tissue culture seedling-exercising method Download PDFInfo
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- CN104839024A CN104839024A CN201510253047.3A CN201510253047A CN104839024A CN 104839024 A CN104839024 A CN 104839024A CN 201510253047 A CN201510253047 A CN 201510253047A CN 104839024 A CN104839024 A CN 104839024A
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- seedling
- transplanting
- hardening
- seedlings
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Abstract
The invention discloses a tissue culture seedling-exercising method. The method comprises the following steps: (1) disinfecting a transplant medium before transplanting, wherein during disinfecting, the transplant medium is baked in a baking oven or kept for 20-30 minutes in a high-pressure sterilizing boiler under the pressure of 15 pounds; (2) exercising seedlings in an enclosed bottle under strong light; (3) exercising the seedlings in an open bottle under strong light, orderly placing tissue culture bottle seedlings on a seedling-exercising frame in order that each row of tissue culture bottle seedlings can be irradiated to form a three-dimensional mode; (4) transplanting test-tube seedlings. Through adoption of the method, defects in the prior art are overcome. The method is easy to operate, is low in cost, and is stable and efficient.
Description
Technical field
The present invention relates to fruit tree virus Prevention Technique field, specifically belong to the method for a kind of group of training hardening.
Background technology
Hardening is that one in tissue cultivating and seedling technology is large crucial, and its success or failure determine the success or failure of whole seedling raising process.Hardening, owing to will keep relatively high air humidity, hardening facility can not carry out ventilation usually, and therefore, the temperature in facility often can be high, often reaches more than 60 degrees Celsius.Compared with the 20-30 degree Celsius of temperature that plant growth is suitable, this is obviously too high, is unfavorable for surviving of plantlet in vitro.In Shading net covering or facility, spray cooling is the current two kinds of common methods overcoming this problem, but under stuffy condition, its effect is very limited.Promote applying of its tissue culture technology, in the present invention, report a kind of hardening off method.Use the method hardening survival rate can be brought up to more than 95%, also having existing in tissue culture procedures, is almost the hardening off method of 100% employing plane bed body, and the area accounting for plane is large, has high input, and work efficiency is slow, and performance tests work difficulty is large.
Summary of the invention
For the problems referred to above, having the object of this invention is to provide the method for a kind of group of training hardening, overcome the deficiencies in the prior art, is a kind of method of simple to operate, with low cost, stability and high efficiency.
The technical solution used in the present invention is as follows:
Group training hardening a method, be made up of following steps:
1) should to transplanting medium disinfection before transplanting;
2) close bottle high light and practice seedling;
3) uncork high light practices seedling, utilizes hardening frame that tissue-culture container seedling is deposited on hardening frame in order, makes the tissue-culture container seedling often arranged accept illumination, forms three-dimensional pattern;
4) transplanting of test-tube plantlet, take out with long forceps is careful when test-tube plantlet shifts out from agar medium, thoroughly clean up root, and avoid damaging root system, directly transplant afterwards or use in the microbicide solutions such as the potassium permanganate of 0.1% ~ 0.3% or carbendazim and clean, then with moving into seedbed or basin alms bowl after clean water, also can wash by water rear carbendazim solution and soaking transplanting after 10 ~ 30 minutes, after planting, drenching sufficient normal root water.
Described sterilization, toasts process in an oven or maintain 20 ~ 30min with 15 pounds of pressure in high-pressure sterilizing pot by transplanting medium.
Described 3) when after taking root or after root system grown substantially, blake bottle is moved on to outdoor and shelter from heat or light and to carry out high light in fluffy or greenhouse and close bottle and practice seedling about 7 ~ 20 days, shade density is preferably 50% ~ 70%.
Compared with the prior art, beneficial effect of the present invention is as follows:
The present invention is simple to operate, with low cost, the method for stability and high efficiency; Saved a large amount of floor area and space, the cost in hardening workshop greatly reduces; The labour intensity of staff reduces, and the working time of inspecting bottles seedling growth shortens greatly, and work efficiency improves more than twice.
Embodiment
Embodiment 1, the method for a kind of group of training hardening, is made up of following steps:
1) should to transplanting medium disinfection before transplanting; Described sterilization, toasts process in an oven or maintain 20 ~ 30min with 15 pounds of pressure in high-pressure sterilizing pot by transplanting medium.
2) close bottle high light and practice seedling;
3) uncork high light practices seedling, utilizes hardening frame that tissue-culture container seedling is deposited on hardening frame in order, makes the tissue-culture container seedling often arranged accept illumination, forms three-dimensional pattern;
4) transplanting of test-tube plantlet, take out with long forceps is careful when test-tube plantlet shifts out from agar medium, thoroughly clean up root (because residual sucrose and nutrition can become the growth medium of potential pathogenic microorganisms, do not wash clean and easily cause transplanted seedling rotten death), and avoid damaging root system, directly transplant afterwards or use in the microbicide solutions such as the potassium permanganate of 0.1% ~ 0.3% or carbendazim and clean, then with moving into seedbed or basin alms bowl after clean water, also can wash by water rear carbendazim solution and soak transplanting after 10 ~ 30 minutes, sufficient normal root water is drenched after planting.
Described 3) when after taking root or after root system grown substantially, blake bottle is moved on to outdoor and shelter from heat or light and to carry out high light in fluffy or greenhouse and close bottle and practice seedling about 7 ~ 20 days, shade density is preferably 50% ~ 70%.
More than show and describe general principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (3)
1. organize a method for training hardening, it is characterized in that, be made up of following steps:
1) should to transplanting medium disinfection before transplanting;
2) close bottle high light and practice seedling;
3) uncork high light practices seedling, utilizes hardening frame that tissue-culture container seedling is deposited on hardening frame in order, makes the tissue-culture container seedling often arranged accept illumination, forms three-dimensional pattern;
4) transplanting of test-tube plantlet, take out with long forceps is careful when test-tube plantlet shifts out from agar medium, thoroughly clean up root (because residual sucrose and nutrition can become the growth medium of potential pathogenic microorganisms, do not wash clean and easily cause transplanted seedling rotten death), and avoid damaging root system, directly transplant afterwards or use in the microbicide solutions such as the potassium permanganate of 0.1% ~ 0.3% or carbendazim and clean, then with moving into seedbed or basin alms bowl after clean water, also can wash by water rear carbendazim solution and soak transplanting after 10 ~ 30 minutes, sufficient normal root water is drenched after planting.
2. the method for according to claim 1 group of training hardening, is characterized in that: described sterilization, transplanting medium is toasted in an oven process or maintain 20 ~ 30min with 15 pounds of pressure in high-pressure sterilizing pot.
3. the method for according to claim 1 group of training hardening, it is characterized in that: described 3) when after taking root or after root system grown substantially, blake bottle is moved on to outdoor to shelter from heat or light and to carry out high light in fluffy or greenhouse and close bottle and practice seedling about 7 ~ 20 days, shade density is preferably 50% ~ 70%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510253047.3A CN104839024A (en) | 2015-05-18 | 2015-05-18 | Tissue culture seedling-exercising method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510253047.3A CN104839024A (en) | 2015-05-18 | 2015-05-18 | Tissue culture seedling-exercising method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104839024A true CN104839024A (en) | 2015-08-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510253047.3A Pending CN104839024A (en) | 2015-05-18 | 2015-05-18 | Tissue culture seedling-exercising method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613238A (en) * | 2015-12-29 | 2016-06-01 | 江苏美尚生态景观股份有限公司 | Seedling hardening method for sequoia sempervirens |
| CN106386144A (en) * | 2016-10-09 | 2017-02-15 | 江苏建康职业学院 | Cultivation method of traditional Chinese virus-free test-tube plantlets |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598098A (en) * | 2013-11-19 | 2014-02-26 | 四川农业大学 | Rapid seedling culturing method for tissue culture of pachyrhizua angulatus |
| CN104106469A (en) * | 2014-06-28 | 2014-10-22 | 赵木华 | Domestication method for tissue culture seedlings |
-
2015
- 2015-05-18 CN CN201510253047.3A patent/CN104839024A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598098A (en) * | 2013-11-19 | 2014-02-26 | 四川农业大学 | Rapid seedling culturing method for tissue culture of pachyrhizua angulatus |
| CN104106469A (en) * | 2014-06-28 | 2014-10-22 | 赵木华 | Domestication method for tissue culture seedlings |
Non-Patent Citations (1)
| Title |
|---|
| 刘进平等: "《热带植物组织培养》", 30 September 2006, 科学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613238A (en) * | 2015-12-29 | 2016-06-01 | 江苏美尚生态景观股份有限公司 | Seedling hardening method for sequoia sempervirens |
| CN105613238B (en) * | 2015-12-29 | 2018-09-21 | 美尚生态景观股份有限公司 | A kind of hardening off method of sequoia sempervirens |
| CN106386144A (en) * | 2016-10-09 | 2017-02-15 | 江苏建康职业学院 | Cultivation method of traditional Chinese virus-free test-tube plantlets |
| CN106386144B (en) * | 2016-10-09 | 2020-03-31 | 江苏建康职业学院 | Cultivation method of traditional Chinese medicine virus-free test-tube plantlet |
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Application publication date: 20150819 |