CN105441457B - 一种与低氮条件下水稻千粒重相关的qtl分子标记方法 - Google Patents
一种与低氮条件下水稻千粒重相关的qtl分子标记方法 Download PDFInfo
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- CN105441457B CN105441457B CN201510939635.2A CN201510939635A CN105441457B CN 105441457 B CN105441457 B CN 105441457B CN 201510939635 A CN201510939635 A CN 201510939635A CN 105441457 B CN105441457 B CN 105441457B
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 70
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 35
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 33
- 235000009566 rice Nutrition 0.000 title claims abstract description 33
- 238000002372 labelling Methods 0.000 title claims abstract description 10
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 32
- 238000009395 breeding Methods 0.000 claims abstract description 23
- 230000001488 breeding effect Effects 0.000 claims abstract description 23
- 201000007602 neuronal ceroid lipofuscinosis 9 Diseases 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims description 11
- 238000012408 PCR amplification Methods 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 17
- 238000000034 method Methods 0.000 abstract description 10
- 241000196324 Embryophyta Species 0.000 abstract description 9
- 230000000306 recurrent effect Effects 0.000 abstract description 4
- 239000003550 marker Substances 0.000 abstract description 3
- 235000013339 cereals Nutrition 0.000 description 14
- 230000000694 effects Effects 0.000 description 8
- 210000000349 chromosome Anatomy 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 3
- 238000007400 DNA extraction Methods 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 238000012098 association analyses Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
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- 238000001514 detection method Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 239000003147 molecular marker Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000012353 t test Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 238000012097 association analysis method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 101150024923 da gene Proteins 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN201510939635.2A CN105441457B (zh) | 2015-12-16 | 2015-12-16 | 一种与低氮条件下水稻千粒重相关的qtl分子标记方法 |
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CN201510939635.2A CN105441457B (zh) | 2015-12-16 | 2015-12-16 | 一种与低氮条件下水稻千粒重相关的qtl分子标记方法 |
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CN105441457A CN105441457A (zh) | 2016-03-30 |
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Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106755325B (zh) * | 2016-11-23 | 2019-06-04 | 中国水稻研究所 | 一种利用分子标记辅助选育中等粒重类型水稻的方法 |
CN109735536A (zh) * | 2019-03-15 | 2019-05-10 | 扬州大学 | 一种控制水稻粒宽和粒厚的新基因位点qGW2-1及其分子标记方法 |
CN111100944A (zh) * | 2020-01-16 | 2020-05-05 | 扬州大学 | 水稻粒型调控基因gw8等位基因的鉴定引物及鉴定方法和应用 |
CN112391494A (zh) * | 2020-12-21 | 2021-02-23 | 江西省农业科学院水稻研究所 | 一种提高水稻氮素利用率的分子育种方法 |
CN113215296B (zh) * | 2021-04-28 | 2022-08-09 | 广西大学 | 水稻芒长基因gna1的分子标记及其鉴定方法和应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100264743B1 (ko) * | 1998-02-06 | 2002-09-17 | 손재근 | Rapdmarker를이용한벼의유묘내냉성에관여하는양적형질유전자지도(qtl)와저온저항성벼품종선발방법 |
CN103421815A (zh) * | 2013-09-06 | 2013-12-04 | 中国农业科学院作物科学研究所 | 一个水稻粒宽新基因qGS5dl及其分子标记方法 |
CN103468716A (zh) * | 2013-09-06 | 2013-12-25 | 中国农业科学院作物科学研究所 | 一种空间诱变产生的水稻粒长新基因qGS3b及其分子标记方法 |
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2015
- 2015-12-16 CN CN201510939635.2A patent/CN105441457B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100264743B1 (ko) * | 1998-02-06 | 2002-09-17 | 손재근 | Rapdmarker를이용한벼의유묘내냉성에관여하는양적형질유전자지도(qtl)와저온저항성벼품종선발방법 |
CN103421815A (zh) * | 2013-09-06 | 2013-12-04 | 中国农业科学院作物科学研究所 | 一个水稻粒宽新基因qGS5dl及其分子标记方法 |
CN103468716A (zh) * | 2013-09-06 | 2013-12-25 | 中国农业科学院作物科学研究所 | 一种空间诱变产生的水稻粒长新基因qGS3b及其分子标记方法 |
Non-Patent Citations (2)
Title |
---|
Identification of Indica rice chromosome segments for the improvement of Japonica inbreds and hybrids;Zhiquan Wang等;《Theor Appl Genet》;20120207;第124卷;第1351-1364页 * |
利用极端材料定位水稻粒形性状数量基因位点;张强等;《作物学报》;20111231;第37卷(第5期);第784-792页 * |
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