CN105392889A - 表达方法 - Google Patents

表达方法 Download PDF

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Publication number
CN105392889A
CN105392889A CN201480024993.4A CN201480024993A CN105392889A CN 105392889 A CN105392889 A CN 105392889A CN 201480024993 A CN201480024993 A CN 201480024993A CN 105392889 A CN105392889 A CN 105392889A
Authority
CN
China
Prior art keywords
expression cassette
sequence
chinese hamster
fibronectin
target polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480024993.4A
Other languages
English (en)
Chinese (zh)
Inventor
F·L·桑德斯
A·L·多兹
A·M·G·贝亚德
B·V·卡拉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Diosynth Biotechnologies UK Ltd
Original Assignee
Fujifilm Diosynth Biotechnologies UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB201308017A external-priority patent/GB201308017D0/en
Priority claimed from GB201320339A external-priority patent/GB201320339D0/en
Application filed by Fujifilm Diosynth Biotechnologies UK Ltd filed Critical Fujifilm Diosynth Biotechnologies UK Ltd
Priority to CN202111508425.XA priority Critical patent/CN114395584B/zh
Publication of CN105392889A publication Critical patent/CN105392889A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3076—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties
    • C07K16/3092—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties against tumour-associated mucins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
CN201480024993.4A 2013-05-03 2014-04-29 表达方法 Pending CN105392889A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111508425.XA CN114395584B (zh) 2013-05-03 2014-04-29 表达方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB201308017A GB201308017D0 (en) 2013-05-03 2013-05-03 Expression process
GB1308017.1 2013-05-03
GB201320339A GB201320339D0 (en) 2013-11-18 2013-11-18 Expression Process
GB1320339.3 2013-11-18
PCT/GB2014/000165 WO2014177826A1 (en) 2013-05-03 2014-04-29 Expression process

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202111508425.XA Division CN114395584B (zh) 2013-05-03 2014-04-29 表达方法

Publications (1)

Publication Number Publication Date
CN105392889A true CN105392889A (zh) 2016-03-09

Family

ID=50639794

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201480024993.4A Pending CN105392889A (zh) 2013-05-03 2014-04-29 表达方法
CN202111508425.XA Active CN114395584B (zh) 2013-05-03 2014-04-29 表达方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202111508425.XA Active CN114395584B (zh) 2013-05-03 2014-04-29 表达方法

Country Status (12)

Country Link
US (3) US9873891B2 (enExample)
EP (1) EP2992104B1 (enExample)
JP (1) JP6545153B2 (enExample)
KR (1) KR102180660B1 (enExample)
CN (2) CN105392889A (enExample)
BR (1) BR112015027373A2 (enExample)
CA (1) CA2910877C (enExample)
DK (1) DK2992104T3 (enExample)
ES (1) ES2732907T3 (enExample)
RU (1) RU2731717C2 (enExample)
SG (1) SG11201508447TA (enExample)
WO (1) WO2014177826A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109837277A (zh) * 2019-03-01 2019-06-04 深圳生生凡非基因技术有限公司 一种cpgk嵌合型启动子、腺相关病毒载体及其应用
CN112980852A (zh) * 2021-05-18 2021-06-18 北京华芢生物技术有限公司 新型冠状病毒b.1.351南非突变株rbd的基因及其应用

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3323826A1 (en) * 2016-11-21 2018-05-23 Danmarks Tekniske Universitet Native chinese hamster ovary cell secretion signal peptides for production of recombinant polypeptides
EP3725877A4 (en) 2017-12-11 2021-02-17 FUJIFILM Corporation ANIMAL CELLS AS WELL AS A METHOD FOR MANUFACTURING THE SAME, AND A METHOD FOR MANUFACTURING TARGET PROTEIN
JP7123168B2 (ja) 2018-12-11 2022-08-22 富士フイルム株式会社 動物細胞、動物細胞の製造方法および目的タンパク質の製造方法
EP4067474A4 (en) 2019-11-29 2023-01-18 FUJIFILM Corporation CELL CULTURE METHOD, ANTIBODY PRODUCTION METHOD, ORGANIC ACID AND ANTIBODY REMOVAL METHOD
WO2022149607A1 (ja) 2021-01-07 2022-07-14 富士フイルム株式会社 細胞の選択方法、装置、及び装置システム
EP4310175A1 (en) 2021-03-17 2024-01-24 FUJIFILM Corporation Cell culturing method, and method for producing useful substance
TW202313127A (zh) 2021-05-27 2023-04-01 美商健生生物科技公司 治療前列腺癌之組合物及方法
EP4410957A1 (en) 2021-09-30 2024-08-07 FUJIFILM Corporation Cell culturing method and production method for useful substance
EP4450609A1 (en) 2021-12-15 2024-10-23 FUJIFILM Corporation Method for producing product, and cell culture apparatus
WO2023190300A1 (ja) 2022-03-28 2023-10-05 富士フイルム株式会社 生産物の生産方法、及び生産物
WO2023190829A1 (ja) 2022-03-31 2023-10-05 富士フイルム株式会社 生産物の製造方法、及び生産物
US20250361524A1 (en) 2022-06-10 2025-11-27 Fujifilm Diosynth Biotechnologies Uk Limited Method for producing cells, method for producing heteromultimeric protein, method for producing bispecific antibody, and method for producing vector set, mammalian cells, CHO cells, and cell pool
EP4596704A1 (en) 2022-09-29 2025-08-06 FUJIFILM Corporation Method for producing product
WO2024248118A1 (ja) 2023-05-31 2024-12-05 富士フイルム株式会社 細胞培養方法および生産物の製造方法
WO2024248119A1 (ja) 2023-05-31 2024-12-05 富士フイルム株式会社 生産物の生産方法
WO2024248120A1 (ja) 2023-05-31 2024-12-05 富士フイルム株式会社 生産物の生産方法
WO2025244971A1 (en) 2024-05-20 2025-11-27 Janssen Biotech, Inc. Compositions and methods for the treatment of prostate cancer

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003093295A2 (en) * 2002-04-30 2003-11-13 University Of North Carolina At Chapel Hill Secretion signal vectors
CN102690834A (zh) * 2006-02-03 2012-09-26 富士胶片戴奥辛思生物技术英国有限公司 表达系统
WO2012173344A2 (ko) * 2011-06-13 2012-12-20 주식회사종근당 Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7186699B2 (en) * 2003-06-03 2007-03-06 Cell Genesys, Inc. Method for treating cancer by vector-mediated delivery of one or more anti-angiogenic or pro-apoptotic genes
US8029783B2 (en) * 2005-02-02 2011-10-04 Genentech, Inc. DR5 antibodies and articles of manufacture containing same
EP1739179A1 (en) * 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
MX2009007139A (es) * 2006-12-28 2009-10-08 Centocor Ortho Biotech Inc Metodos y vectores para generar inmunoglobulinas no sialiladas.
KR101618767B1 (ko) * 2008-09-02 2016-05-09 한국생명공학연구원 외래단백질을 고효율로 생산하는 리더서열
US8529903B2 (en) * 2008-12-31 2013-09-10 Bharat Serums And Vaccines Ltd. Anti-RhD monoclonal antibodies
EP2810652A3 (en) 2009-03-05 2015-03-11 AbbVie Inc. IL-17 binding proteins
GB0905331D0 (en) * 2009-03-27 2009-05-13 Avecia Biolog Ltd Expression process

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003093295A2 (en) * 2002-04-30 2003-11-13 University Of North Carolina At Chapel Hill Secretion signal vectors
CN102690834A (zh) * 2006-02-03 2012-09-26 富士胶片戴奥辛思生物技术英国有限公司 表达系统
WO2012173344A2 (ko) * 2011-06-13 2012-12-20 주식회사종근당 Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LUCIA BALDI ET AL.,: ""Recombinant protein production by large-scale transient"", 《BIOTECHNOL LETT》 *
范翠英等: ""重组蛋白表达系统的研究进展"", 《生物技术》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109837277A (zh) * 2019-03-01 2019-06-04 深圳生生凡非基因技术有限公司 一种cpgk嵌合型启动子、腺相关病毒载体及其应用
CN112980852A (zh) * 2021-05-18 2021-06-18 北京华芢生物技术有限公司 新型冠状病毒b.1.351南非突变株rbd的基因及其应用
CN112980852B (zh) * 2021-05-18 2021-08-13 北京华芢生物技术有限公司 新型冠状病毒b.1.351南非突变株rbd的基因及其应用

Also Published As

Publication number Publication date
CA2910877C (en) 2021-08-03
RU2015151626A (ru) 2017-06-08
KR20160003705A (ko) 2016-01-11
US20180127777A1 (en) 2018-05-10
US11306323B2 (en) 2022-04-19
US20160076051A1 (en) 2016-03-17
US9873891B2 (en) 2018-01-23
JP6545153B2 (ja) 2019-07-17
ES2732907T3 (es) 2019-11-26
BR112015027373A2 (pt) 2017-08-29
RU2731717C2 (ru) 2020-09-08
RU2015151626A3 (enExample) 2018-03-20
JP2016517691A (ja) 2016-06-20
EP2992104A1 (en) 2016-03-09
CN114395584A (zh) 2022-04-26
CA2910877A1 (en) 2014-11-06
KR102180660B1 (ko) 2020-11-19
US20220213500A1 (en) 2022-07-07
WO2014177826A1 (en) 2014-11-06
CN114395584B (zh) 2025-01-28
DK2992104T3 (da) 2019-07-15
EP2992104B1 (en) 2019-04-17
SG11201508447TA (en) 2015-11-27

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SE01 Entry into force of request for substantive examination
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Application publication date: 20160309