CN105340712B - A kind of greenhouse live propagation method of Herba Dendrobii - Google Patents

A kind of greenhouse live propagation method of Herba Dendrobii Download PDF

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Publication number
CN105340712B
CN105340712B CN201510881595.0A CN201510881595A CN105340712B CN 105340712 B CN105340712 B CN 105340712B CN 201510881595 A CN201510881595 A CN 201510881595A CN 105340712 B CN105340712 B CN 105340712B
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herba dendrobii
seed
live
seedbed
seedling
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CN201510881595.0A
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Chinese (zh)
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CN105340712A (en
Inventor
王喆之
王世强
牛俊峰
党凯凯
王尚萍
张慧
杨咪
曹达
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陕西师范大学
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor

Abstract

The invention discloses the live propagation method in greenhouse of a kind of Herba Dendrobii, the method is with Herba Dendrobii fruit pod as material, first pass through specific auximone and process Seeds of Dendrobium Candidum, and select specific live substrate, make live after the emergence rate of Seeds of Dendrobium Candidum reach more than 80%, then reach nearly 100% by the Herba Dendrobii seedling percent after selecting specific transplanting medium to make transplanting.

Description

A kind of greenhouse live propagation method of Herba Dendrobii
Technical field
The invention belongs to medicinal plants artificial planting technique field, be specifically related to the temperature of Endangered Medicinal Herb Herba Dendrobii The live propagation method in room.
Background technology
Herba Dendrobii (Dendrobium officinale Kimura et Migo) is that the orchid family Dendrobium perennial herb is planted Thing, is distributed mainly on the ground such as Yunnan, Anhui, Guizhou, Zhejiang, Jiangxi, for a kind of rare Chinese crude drug.Because of it There is nourishing YIN and clearing away heat, effect such as stomach reinforcing, nourishing the lung to arrest cough of promoting the production of body fluid, rank " first of China's nine big Herba mesonae chinensis ", and recorded Enter 2015 editions pharmacopeia.Its primary medicinal component is that Dendrobium officinale polysaccharide, alkaloid, aminoacid become with other trace Point.Modern pharmacology shows, Herba Dendrobii have antitumor, antioxidation, defying age, enhancing body immunity, Effect and the effects of expansion blood vessel such as suppression tumor and reduction blood glucose, its special pharmacological effect effect, make this plant The wild resource of thing receives artificial extensive, gopher, thus makes the wild resource of Herba Dendrobii in short supply, The most endangered.
Seeds of Dendrobium Candidum does not has endosperm, and germination percentage is low under field conditions (factors), poor growth, and artificial self-pollination becomes Power is relatively low, and growing environment is required harsh (under native state, primary growth is in overhanging cliff), cannot expire The market demand that foot is growing.Tissue culture is the important channel that current seedlings of Dendrobium officinale produces, but tissue The method growing-seedling period cultivated is long, is difficult to domestication, and transplanting survival rate is the highest, and relatively costly.
Summary of the invention
The technical problem to be solved is to overcome existing dendrobium candidum tissue to cultivate growing-seedling period length, be difficult to Domestication, the shortcoming that transplanting survival rate is the highest, cost is high, it is provided that one can save artificial, with low cost, breeding effect The live propagation method in Herba Dendrobii greenhouse that rate is high.
Solve the technical scheme that above-mentioned technical problem used to be made up of following step:
1, artificial pollination and Seed Deposit
Select healthy and strong, disease-free Herba Dendrobii, carry out artificial pollination, artificial pollination 4~5 months when blooming After gather and grow full fruit pod, In Shade dry, be subsequently placed in 0~4 DEG C of preservation.
2, the arrangement in seedbed
Select the seedbed of reinforced cement greenhouse booth, seedbed length 30~40m, wide 1.5~2m, high 0.8~1.2m, will mould Material paper is laid on seedbed, and the surrounding of plastic paper is fixed on edge, seedbed, it is ensured that can keep 1~2cm height on seedbed Water.
3, the process of live substrate
By medicinal residues, coconut palm bran, the peat soil that fermented by volume for 1:(5~10): (10~20) mix homogeneously, Gained mixture and carbendazim, phoxim mix for 1000:3:3 by volume, are configured to live substrate;Will be straight Broadcast substrate and fill seedling culture hole plate, after flattening compacting, seedling culture hole plate is placed on seedbed, waters permeable to live substrate, Soak 7~10 days.
4, the process of seed
Seed is taken out in the Herba Dendrobii fruit pod strip off of step 1 preservation, seed is water-soluble at 2~5mg/L naphthalene acetic acids The mixed liquor that volume ratio is 1:1 of liquid and 5~10mg/L paclobutrazol aqueous solutions soaks 3~5 hours, then uses Without phosphorus filter paper filtering also dries seed;By Pulvis Talci and naphthalene acetic acid, paclobutrazol 5000:(1 in mass ratio~3): (1~3) mix homogeneously, gained mixture is fully mixed thoroughly for 100:1 in mass ratio with the seed dried.
5, sowing management
The Seeds of Dendrobium Candidum mixed in step 4 is placed in 60 mesh dusting covers, taps dusting cover edge and make seed uniform Sowing in the live substrate in seedling culture hole plate, sowing density is 3~5/cm2, then cover on seedling culture hole plate Preservative film, the temperature controlled in canopy is 25~35 DEG C, waters once every 3 days after sowing one week, keeps in air Moisture is 80%~90%;Treat that Herba Dendrobii seedling grows a piece of true leaf, throw off preservative film, spray weekly battalion Nutrient solution 1 time;Treat that seedling grows 3~4 true leaves, sprayed nutritional solution and diamidogen compound fertilizer aqueous solution each 1 every 5 days Secondary.
6, transplantation of seedlings and management are planted
Using Vermiculitum and coconut palm bran, Cortex Pini volume ratio as 1:(5~10): the mixture of (3~5) as transplanting medium, Transplanting medium is filled seedling culture hole plate, after flattening compacting, seedling culture hole plate is placed on seedbed;Treat Herba Dendrobii seedling After growing 6~7 true leaves, select the transplanting that seedling replanting anosis, healthy and strong, of uniform size enters in seedling culture hole plate In substrate, every 3~5 strains are one clump, plantation line-spacing and clump away from for 8~12cm, control the temperature in canopy be 25~ 35 DEG C, watering every 3 days 1 time, keeping atmospheric water content is 60%~80%, and sprays battalion every 3 days Nutrient solution 1 time, monthly sprinkling streptomycin sulfate 1 time, air themperature costs double-layered sunshade net more than 35 DEG C to booth.
In above-mentioned steps 3, preferably medicinal residues, coconut palm bran, the peat soil that fermented are mixed for 1:10:15 by volume Uniformly, gained mixture and carbendazim, phoxim mix for 1000:3:3 by volume, are configured to live substrate.
In above-mentioned steps 4, preferably seed is taken out in the Herba Dendrobii fruit pod strip off of step 1 preservation, seed is existed 3mg/L naphthalene acetic acid aqueous solution and the mixed liquor that volume ratio is 1:1 of 8mg/L paclobutrazol aqueous solution soak 3~5 Hour, then with without phosphorus filter paper filtering and dry seed;By Pulvis Talci with naphthalene acetic acid, paclobutrazol in mass ratio 5000:2:2 mix homogeneously, gained mixture is fully mixed thoroughly for 100:1 in mass ratio with the seed dried.
In above-mentioned steps 6, preferably using Vermiculitum and coconut palm bran, the Cortex Pini volume ratio mixture as 1:8:3 as transplanting Substrate.
Above-mentioned nutritional solution is that after being mixed for 80:7:13 by volume with trace element, iron salt by a great number of elements, use is steamed Distilled water 300 times of mixed liquors obtained of dilution, described a great number of elements is by 30.300g KNO3、20.415g KH2PO4、6.000g NH4NO3、9.9g(NH4)2SO4、7.35g MgSO4·7H2O、1.659g CaCl2With steaming Distilled water is settled to the mixed liquor that 1L obtains, and described trace element is by 9.2745g H3BO3、5.433g MnSO4、 0.408g ZnCl2、0.749g CuSO4·5H2O、0.1236g Na2MoO4With distilled water be settled to that 1L obtains mixed Closing liquid, described iron salt is by 16.68g FeSO4·7H2O, 22.32g EDTA 2Na distilled water is settled to 1L The mixed liquor obtained.
The present invention first passes through specific auximone and processes Seeds of Dendrobium Candidum, and selects specific live substrate, Make live after the emergence rate of Seeds of Dendrobium Candidum can reach more than 80%, and emerge rapidly, seed sowing 1 month Rear Herba Dendrobii seedling can grow 1 true leaf, then by the iron sheet after selecting specific transplanting medium to make transplanting Herba Dendrobii seedling percent reaches nearly 100%, and plant strain growth is rapid, grows fine.
The present invention is simple, with low cost, and emergence rate is high, and growing-seedling period is short, suits large area to popularize, can be complete Full up sufficient large area, the needs of scale Herba Dendrobii nursery, have the double meaning produced with economically, for ferrum The quick sexual propagation of skin Herba Dendrobii opens up a new way.
Accompanying drawing explanation
Fig. 1 is the Seeds of Dendrobium Candidum photo of live 3 months in embodiment 1.
Fig. 2 is the Herba Dendrobii seedling replanting photo of 1 month in embodiment 1.
Fig. 3 is the Seeds of Dendrobium Candidum photo of live 3 months in embodiment 2.
Fig. 4 is the Herba Dendrobii seedling replanting photo of 1 month in embodiment 2.
Fig. 5 is the Seeds of Dendrobium Candidum photo of live 3 months in embodiment 3.
Fig. 6 is the Herba Dendrobii seedling replanting photo of 1 month in embodiment 3.
Detailed description of the invention
The present invention is described in more detail with embodiment below in conjunction with the accompanying drawings, but protection scope of the present invention not only limits In these embodiments.
Nutritional solution used in example below is to be by volume with trace element, iron salt by a great number of elements The mixed liquor that obtains again with distilled water diluting 300 after 80:7:13 mixing, wherein said a great number of elements be by 30.300g KNO3、20.415g KH2PO4、6.000g NH4NO3、9.9g(NH4)2SO4、7.35g MgSO4·7H2O、1.659g CaCl2Being settled to, with distilled water, the mixed liquor that 1L obtains, described trace element is By 9.2745g H3BO3、5.433g MnSO4、0.408g ZnCl2、0.749g CuSO4·5H2O、0.1236g Na2MoO4Being settled to, with distilled water, the mixed liquor that 1L obtains, described iron salt is by 16.68g FeSO4·7H2O、 22.32g EDTA 2Na distilled water is settled to the mixed liquor that 1L obtains.
Embodiment 1
1, artificial pollination and Seed Deposit
Selecting healthy and strong, disease-free Herba Dendrobii, carry out artificial pollination when blooming, artificial pollination was adopted after 5 months Full fruit pod is educated in transmitting-receiving, In Shade dries, and is subsequently placed in 4 DEG C of preservations.
2, the arrangement in seedbed
Select the seedbed of reinforced cement greenhouse booth, the long 30m in seedbed, wide 1.5m, high 1.2m, plastic paper is laid in On seedbed, the fixing pin chips of surrounding of plastic paper is at edge, seedbed, it is ensured that can keep water high for 2cm on seedbed.
3, the process of live substrate
By medicinal residues, coconut palm bran, the peat soil that fermented by volume for 1:10:15 mix homogeneously, gained mixture with Carbendazim, phoxim mix for 1000:3:3 by volume, are configured to live substrate;Live substrate is filled and educates Seedling hole tray, after flattening compacting, is placed on seedling culture hole plate on seedbed, waters permeable to live substrate, soak 7 days.
4, the process of seed
Seed is taken out in the fruit pod strip off of the Herba Dendrobii of step 1 preservation, by seed 3mg/L naphthalene acetic acid aqueous solution with The mixed liquor that volume ratio is 1:1 of 8mg/L paclobutrazol aqueous solution soaks 4 hours, then uses without phosphorus filter paper filtering And dry seed;Being mixed homogeneously with naphthalene acetic acid, paclobutrazol 5000:1:2 in mass ratio by Pulvis Talci, gained mixes Thing is fully mixed thoroughly for 100:1 in mass ratio with the seed dried.
5, sowing management
The Seeds of Dendrobium Candidum mixed in step 4 is placed in 60 mesh dusting covers, taps dusting cover edge and make seed uniform Sowing in the live substrate in seedling culture hole plate, sowing density is 3~5/cm2, then cover on seedling culture hole plate Preservative film, the temperature controlled in canopy is 25~35 DEG C, waters once every 3 days after sowing one week, keeps in air Moisture is 80%~90%;After sowing 30 days, Herba Dendrobii seedling grows a piece of true leaf, throws off preservative film, Spray nutritional solution 1 time weekly;After sowing 3 months, its seedling has grown 2~3 true leaves, as seen from Figure 1, Emerging of Herba Dendrobii is fine, and emergence rate can reach more than 85%, and robust plant, individuality is relatively big, and bulb diameter can Reach 0.5cm;Treat that seedling grows 3~4 true leaves, sprayed nutritional solution and diamidogen compound fertilizer aqueous solution each 1 every 5 days Secondary.
6, transplantation of seedlings and management are planted
Using Vermiculitum and coconut palm bran, the Cortex Pini volume ratio mixture as 1:8:3 as transplanting medium, transplanting medium is filled Full seedling culture hole plate, after flattening compacting, is placed on seedling culture hole plate on seedbed;Sow about 8 months, Herba Dendrobii children Seedling grows 6~7 true leaves, selects the transplanting that seedling replanting anosis, healthy and strong, of uniform size enters in seedling culture hole plate In substrate, every 3~5 strains are one clump, and plantation line-spacing and clump are away from for 10cm, and the temperature controlled in canopy is 25~35 DEG C, Watering every 3 days 1 time, keeping atmospheric water content is 60%~80%, and sprays nutritional solution 1 every 3 days Secondary, monthly sprinkling streptomycin sulfate 1 time, air themperature costs double-layered sunshade net more than 35 DEG C to booth.By scheming 2 is visible, and Herba Dendrobii seedling replanting is after one month, and blade is bud green, and plant grows fine, and growth is rapidly.
Embodiment 2
1, artificial pollination and Seed Deposit
Selecting healthy and strong, disease-free Herba Dendrobii, carry out artificial pollination when blooming, artificial pollination was adopted after 5 months Full fruit pod is educated in transmitting-receiving, In Shade dries, and is subsequently placed in 0 DEG C of preservation.
2, the arrangement in seedbed
Select the seedbed of reinforced cement greenhouse booth, the long 40m in seedbed, wide 2m, high 1m, plastic paper is laid in seedbed On, the fixing pin chips of surrounding of plastic paper is at edge, seedbed, it is ensured that can keep water high for 1cm on seedbed.
3, the process of live substrate
By medicinal residues, coconut palm bran, the peat soil that fermented by volume for 1:10:10 mix homogeneously, gained mixture with Carbendazim, phoxim mix for 1000:3:3 by volume, are configured to live substrate;Live substrate is filled and educates Seedling hole tray, after flattening compacting, is placed on seedling culture hole plate on seedbed, waters permeable to live substrate, soak 10 days.
4, the process of seed
Seed is taken out in the fruit pod strip off of the Herba Dendrobii of step 1 preservation, by seed 5mg/L naphthalene acetic acid aqueous solution with The mixed liquor that volume ratio is 1:1 of 5mg/L paclobutrazol aqueous solution soaks 5 hours, then uses without phosphorus filter paper filtering And dry seed;Being mixed homogeneously with naphthalene acetic acid, paclobutrazol 5000:2:1 in mass ratio by Pulvis Talci, gained mixes Thing is fully mixed thoroughly for 100:1 in mass ratio with the seed dried.
5, sowing management
The Seeds of Dendrobium Candidum mixed in step 4 is placed in 60 mesh dusting covers, taps dusting cover edge and make seed uniform Sowing in the live substrate in seedling culture hole plate, sowing density is 3~5/cm2, then cover on seedling culture hole plate Preservative film, the temperature controlled in canopy is 25~35 DEG C, waters once every 3 days after sowing one week, keeps in air Moisture is 80%~90%;After sowing 35~40 days, Herba Dendrobii seedling grows a piece of true leaf, throws off guarantor Fresh film, sprays nutritional solution 1 time weekly;After sowing 3 months, its seedling has grown 2 true leaves, as seen from Figure 3, Emerging of Herba Dendrobii is preferable, and emergence rate can reach more than 80%, and robust plant, individuality is relatively big, and bulb diameter can Reach 0.4cm;Treat that seedling grows 3~4 true leaves, sprayed nutritional solution and diamidogen compound fertilizer aqueous solution each 1 every 5 days Secondary.
6, transplantation of seedlings and management are planted
Using Vermiculitum and coconut palm bran, the Cortex Pini volume ratio mixture as 1:10:3 as transplanting medium, by transplanting medium Fill seedling culture hole plate, after flattening compacting, seedling culture hole plate is placed on seedbed;Sow about 9 months, Herba Dendrobii After seedling grows 6~7 true leaves, seedling replanting anosis, healthy and strong, of uniform size is selected to enter in seedling culture hole plate In transplanting medium, every 3~5 strains are one clump, plantation line-spacing and clump away from for 10cm, control the temperature in canopy be 25~ 35 DEG C, watering every 3 days 1 time, keeping atmospheric water content is 60%~80%, and sprays battalion every 3 days Nutrient solution 1 time, monthly sprinkling streptomycin sulfate 1 time, air themperature costs double-layered sunshade net more than 35 DEG C to booth. From fig. 4, it can be seen that Herba Dendrobii seedling replanting is after one month, blade is light green, and plant growing way is preferable, grows rapider.
Embodiment 3
1, artificial pollination and Seed Deposit
Selecting healthy and strong, disease-free Herba Dendrobii, carry out artificial pollination when blooming, artificial pollination was adopted after 4 months Full fruit pod is educated in transmitting-receiving, In Shade dries, and is subsequently placed in 4 DEG C of preservations.
2, the arrangement in seedbed
Select the seedbed of reinforced cement greenhouse booth, the long 35m in seedbed, wide 1.8m, high 0.8m, plastic paper is laid in On seedbed, the fixing pin chips of surrounding of plastic paper is at edge, seedbed, it is ensured that can keep water high for 1.5cm on seedbed.
3, the process of live substrate
By medicinal residues, coconut palm bran, the peat soil that fermented by volume for 1:5:20 mix homogeneously, gained mixture with Carbendazim, phoxim mix for 1000:3:3 by volume, are configured to live substrate;Live substrate is filled and educates Seedling hole tray, after flattening compacting, is placed on seedling culture hole plate on seedbed, waters permeable to live substrate, soak 10 days.
4, the process of seed
Seed is taken out in the fruit pod strip off of the Herba Dendrobii of step 1 preservation, by seed 2mg/L naphthalene acetic acid aqueous solution with The mixed liquor that volume ratio is 1:1 of 10mg/L paclobutrazol aqueous solution soaks 3 hours, then by without phosphorus filter paper mistake Filter and dry seed;Being mixed homogeneously with naphthalene acetic acid, paclobutrazol 5000:1:3 in mass ratio by Pulvis Talci, gained mixes Compound is fully mixed thoroughly for 100:1 in mass ratio with the seed dried.
5, sowing management
The Seeds of Dendrobium Candidum mixed in step 4 is placed in 60 mesh dusting covers, taps dusting cover edge and make seed uniform Sowing in the live substrate in seedling culture hole plate, sowing density is 3~5/cm2, then cover on seedling culture hole plate Preservative film, the temperature controlled in canopy is 25~35 DEG C, waters once every 3 days after sowing one week, keeps in air Moisture is 80%~90%;After sowing 35~40 days, Herba Dendrobii seedling grows a piece of true leaf, throws off guarantor Fresh film, sprays nutritional solution 1 time weekly;After sowing 3 months, its seedling has grown 2 true leaves, as seen from Figure 5, Emerging preferably of Herba Dendrobii, emergence rate can reach more than 80%;Treat that seedling grows 3~4 true leaves, every 5 It sprays nutritional solution and each 1 time of diamidogen compound fertilizer aqueous solution.
6, transplantation of seedlings and management are planted
Using Vermiculitum and coconut palm bran, the Cortex Pini volume ratio mixture as 1:5:5 as transplanting medium, transplanting medium is filled Full seedling culture hole plate, after flattening compacting, is placed on seedling culture hole plate on seedbed;Sow about 10 months, Herba Dendrobii After seedling grows 7 true leaves, select the transplanting that seedling replanting anosis, healthy and strong, of uniform size enters in seedling culture hole plate In substrate, every 3~5 strains are one clump, and plantation line-spacing and clump are away from for 10cm, and the temperature controlled in canopy is 25~35 DEG C, Watering every 3 days 1 time, keeping atmospheric water content is 60%~80%, and sprays nutritional solution 1 every 3 days Secondary, monthly sprinkling streptomycin sulfate 1 time, air themperature costs double-layered sunshade net more than 35 DEG C to booth.By scheming 6 is visible, and Herba Dendrobii seedling replanting is after one month, and plant growing way is preferable, and growth is very fast.

Claims (5)

1. the live propagation method in the greenhouse of a Herba Dendrobii, it is characterised in that it is made up of following step:
(1) artificial pollination and Seed Deposit
Select healthy and strong, disease-free Herba Dendrobii, carry out artificial pollination, artificial pollination 4~5 months when blooming After gather and grow full fruit pod, In Shade dry, be subsequently placed in 0~4 DEG C of preservation;
(2) arrangement in seedbed
Select the seedbed of reinforced cement greenhouse booth, seedbed length 30~40m, wide 1.5~2m, high 0.8~1.2m, will mould Material paper is laid on seedbed, and the surrounding of plastic paper is fixed on edge, seedbed, it is ensured that can keep 1~2cm height on seedbed Water;
(3) process of live substrate
By medicinal residues, coconut palm bran, the peat soil that fermented by volume for 1:(5~10): (10~20) mix homogeneously, Gained mixture and carbendazim, phoxim mix for 1000:3:3 by volume, are configured to live substrate;Will be straight Broadcast substrate and fill seedling culture hole plate, after flattening compacting, seedling culture hole plate is placed on seedbed, waters permeable to live substrate, Soak 7~10 days;
(4) process of seed
The Herba Dendrobii fruit pod strip off of step (1) preservation is taken out seed, by seed at 2~5mg/L naphthalene acetic acid water Immersion 3~5 hours in the mixed liquor that volume ratio is 1:1 of solution and 5~10mg/L paclobutrazol aqueous solutions, then With without phosphorus filter paper filtering and dry seed;By Pulvis Talci and naphthalene acetic acid, paclobutrazol 5000:(1 in mass ratio~3): (1~3) mix homogeneously, gained mixture is fully mixed thoroughly for 100:1 in mass ratio with the seed dried;
(5) sowing management
The Seeds of Dendrobium Candidum mixed in step (4) is placed in 60 mesh dusting covers, taps dusting cover edge and make seed equal In the even live substrate sowed in seedling culture hole plate, sowing density is 3~5/cm2, then in seedling culture hole plate overlying Lid preservative film, the temperature controlled in canopy is 25~35 DEG C, waters once every 3 days after sowing one week, keeps air Middle moisture is 80%~90%;Treat that Herba Dendrobii seedling grows a piece of true leaf, throw off preservative film, spray weekly Nutritional solution 1 time;Treat that seedling grows 3~4 true leaves, sprayed nutritional solution and diamidogen compound fertilizer aqueous solution every 5 days Each 1 time;
(6) transplantation of seedlings and management are planted
Using Vermiculitum and coconut palm bran, Cortex Pini volume ratio as 1:(5~10): the mixture of (3~5) as transplanting medium, Transplanting medium is filled seedling culture hole plate, after flattening compacting, seedling culture hole plate is placed on seedbed;Treat Herba Dendrobii seedling After growing 6~7 true leaves, select the transplanting that seedling replanting anosis, healthy and strong, of uniform size enters in seedling culture hole plate In substrate, every 3~5 strains are one clump, plantation line-spacing and clump away from for 8~12cm, control the temperature in canopy be 25~ 35 DEG C, watering every 3 days 1 time, keeping atmospheric water content is 60%~80%, and sprays battalion every 3 days Nutrient solution 1 time, monthly sprinkling streptomycin sulfate 1 time, air themperature costs double-layered sunshade net more than 35 DEG C to booth.
The live propagation method in greenhouse of Herba Dendrobii the most according to claim 1, it is characterised in that: described In step (3), by medicinal residues, coconut palm bran, the peat soil that fermented by volume for 1:10:15 mix homogeneously, institute Obtain mixture to mix for 1000:3:3 by volume with carbendazim, phoxim, be configured to live substrate.
The live propagation method in greenhouse of Herba Dendrobii the most according to claim 1, it is characterised in that: described In step (4), the Herba Dendrobii fruit pod strip off of step (1) preservation is taken out seed, by seed at 3mg/L naphthalene Acetic acid aqueous solution and immersion in the mixed liquor that volume ratio is 1:1 of 8mg/L paclobutrazol aqueous solution 3~5 hours, so Afterwards with without phosphorus filter paper filtering and dry seed;Pulvis Talci is mixed with naphthalene acetic acid, paclobutrazol 5000:2:2 in mass ratio Closing uniformly, gained mixture is fully mixed thoroughly for 100:1 in mass ratio with the seed dried.
The live propagation method in greenhouse of Herba Dendrobii the most according to claim 1, it is characterised in that: described In step (6), using Vermiculitum and coconut palm bran, the Cortex Pini volume ratio mixture as 1:8:3 as transplanting medium.
The live propagation method in greenhouse of Herba Dendrobii the most according to claim 1, it is characterised in that: described Nutritional solution be mixed for 80:7:13 by volume with trace element, iron salt by a great number of elements after use distilled water diluting 300 times of mixed liquors obtained, described a great number of elements is by 30.300g KNO3、20.415g KH2PO4、6.000g NH4NO3、9.9g(NH4)2SO4、7.35g MgSO4·7H2O、1.659g CaCl2It is settled to 1L with distilled water obtain The mixed liquor arrived, described trace element is by 9.2745g H3BO3、5.433g MnSO4、0.408g ZnCl2、 0.749g CuSO4·5H2O、0.1236g Na2MoO4It is settled to the mixed liquor that 1L obtains with distilled water, described Iron salt is by 16.68g FeSO4·7H2O, 22.32g EDTA 2Na distilled water is settled to the mixed liquor that 1L obtains.
CN201510881595.0A 2015-12-03 2015-12-03 A kind of greenhouse live propagation method of Herba Dendrobii CN105340712B (en)

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CN107046988A (en) * 2017-03-21 2017-08-18 农凯峰 The green cultural method of dendrobium candidum
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CN107046989A (en) * 2017-03-21 2017-08-18 农凯峰 The cultural method of dendrobium candidum
CN107079679A (en) * 2017-03-21 2017-08-22 农凯峰 The high-yield planting method of dendrobium candidum
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