CN104082113B - Method for recycling rhizoma bletillae nursing media and directly seeding dendrobium officinale - Google Patents
Method for recycling rhizoma bletillae nursing media and directly seeding dendrobium officinale Download PDFInfo
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- CN104082113B CN104082113B CN201410304755.0A CN201410304755A CN104082113B CN 104082113 B CN104082113 B CN 104082113B CN 201410304755 A CN201410304755 A CN 201410304755A CN 104082113 B CN104082113 B CN 104082113B
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Abstract
The invention discloses a method for recycling rhizoma bletillae nursing media and directly seeding dendrobium officinale. Media obtained after the rhizoma bletillae is directly seeded the first year are dried, sieved and then evenly mixed with fermented bark powder and carbendazim, then direct seeding media of the dendrobium officinale are prepared, and the dendrobium officinale is directly seeded through the media, so that the rhizoma bletillae nursing media are recycled, and the defect that the media can not be recycled after the rhizoma bletillae is directly seeded is overcome. The method is easy to implement, low in cost, high in speed of emergence of seedlings, low in dependence on seasons and territories, convenient to popularize and capable of completely meeting the requirement for large-area and large-scale seedling cultivation. The method overcomes the shortcoming that as dendrobium officinale seeds do not have endosperm, sprouting of the dendrobium officinale seeds is difficult under natural conditions, and a new path is developed for rapid propagation of dendrobium plants.
Description
Technical field
The invention belongs to medicinal plant artificial planting technique field, be specifically related to the artificial method for planting of dendrobium candidum.
Background technology
Dendrobium candidum (Dendrobium officinale) is the orchid family Dendrobium herbaceos perennial, it is as a kind of traditional rare Chinese medicine, because of its there is reinforcing stomach reg fluid, nourishing Yin and clearing heat, the effect such as moisten the lung and relieve the cough, ranking first of " Chinese nine immortals grass ", is 2010 editions pharmacopeia medications.
Seeds of Dendrobium Candidum is minimum, without endosperm, germination rate is low, and its reproduction rate is low, poor growth, and requires harsh (under natural endowment, primary growth is on overhanging cliff and rotten wood) growing environment, and natural production is very rare.Under conventional conditions, breeding is by plant division, cuttage, but the reproduction coefficient of these two kinds of modes is extremely low, and in production, mainly produce seedling by invisible modess of reproduction such as tissue cultures at present, but output is very limited, and can not form scale, the final imbalance between supply and demand solving dendrobium candidum also needs to be undertaken by direct seeding technique.
At present, the direct seeding technique of bletilla is ripe, but in live process, its old matrix continues live bletilla and occurred that percentage of seedgermination is low and plant strain growth is slow and even not long situation.Orchid needs the help of endogenetic fungus in germination process, cause the reason of above-mentioned bletilla seed germination may be its as old matrix, wherein the change of microbial population is not suitable for the sprouting stage of bletilla, therefore causes a large amount of wastes of old matrix.
Summary of the invention
Technical problem to be solved by this invention be to overcome dendrobium candidum tradition modes of reproduction and division propagation reproduction coefficient low, tissue culture method high in cost of production shortcoming, provides the live propagation method of a kind of dendrobium candidum with low cost, that reproductive efficiency is high.
Solve the problems of the technologies described above adopted technical scheme to be made up of following step:
1, the matrix after live bletilla is processed and sabot
Matrix after live for First Year bletilla is dried, sieved, then by the matrix after sieving and the bark powder of fermenting by volume 5 ~ 10:1 mix, gained mixture and carbendazim are by volume for 1000:5 mixes, be mixed with the live matrix of dendrobium candidum, live for dendrobium candidum matrix is loaded in the porose square plate in bottom, be filled to 2/3 place of the square plate degree of depth, flatten compacting, then square plate is placed on the seedbed in plastic tunnel, the water of 1 ~ 2cm degree of depth is marked with in advance in seedbed, water to matrix, matrix is thoroughly irrigated.
The matrix of the live bletilla of above-mentioned First Year is the bark powder, Nutrition Soil, the river sand that fermented be the mixture of 1 ~ 8:5 ~ 10:1 and the volume ratio of carbendazim is by volume the mixture of 1000:1.
2, sowing and nursery
Seeds of Dendrobium Candidum is placed in 4 DEG C of refrigerator low temperature treatment 24 hours, then itself and talcum powder is fully mixed thoroughly for 1:10 in mass ratio, evenly sowed on square plate by the seed mixed, sowing density is 3 ~ 5g/m
2, at square plate surface coverage one deck preservative film, sow in one week and do not water, watered once by the space between square plate and square plate every 3 days later.
3, strong sprout
Treat that dendrobium candidum seedling grows two panels true leaf, spray growth-promoting liquid 1 time weekly, and sprayed nutrient solutions and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution every 3 days.
The methyl α-naphthyl acetate aqueous solution of above-mentioned growth-promoting liquid to be quality-volumetric concentration be 0.0005 ~ 0.002mg/L; Nutrient solution mixes the rear mixed liquor doubly obtained with distilled water diluting 10000 for 50:3:15 by macroelement by volume with micro-, molysite, and described macroelement is by 60.6g KNO
3, 40.83g KH
2pO
4, 12g NH
4nO
3, 19.8g (NH
4)
2sO
4, 14.7g, MgSO
47H
2o, 3.318g CaCl
2be settled to distilled water the mixed liquor that 1L obtains, described trace element is by 9.2745g H
3bO
3, 5.433g MnSO
4, 0.408g ZnCl
2, 0.749gCuSO
45H
2o, 0.1236g Na
2moO
4be settled to distilled water the mixed liquor that 1L obtains, described molysite is by 16.68g FeSO
47H
2o, 22.32g EDTA2Na distilled water is settled to the mixed liquor that 1L obtains.
In above-mentioned step 1, preferably the matrix after live for First Year bletilla is dried, sieved, then by the matrix after sieving and the bark powder of fermenting by volume 5:1 mix, gained mixture and carbendazim are by volume for 1000:5 mixes, be mixed with the live matrix of dendrobium candidum, the matrix of the live bletilla of wherein said First Year be fermented bark powder, Nutrition Soil, river sand is by volume for the mixture of 2:5:1 and the volume ratio of carbendazim are the mixture of 1000:1.
In above-mentioned step 3, growth-promoting liquid preferred mass-volumetric concentration is the methyl α-naphthyl acetate aqueous solution of 0.001mg/L, and the quality-volumetric concentration of di(2-ethylhexyl)phosphate amine aqueous solution is 0.2g/L.
Instant invention overcomes dendrobium candidum plant because seed is tiny, do not have endosperm, extremely difficult predicament of sprouting under field conditions (factors), for dendrobium candidum provides a new modes of reproduction, also the utilization again to the live matrix of bletilla is completed on producing, not only there is the double meaning produced with economically, traditional hydroponics reproduction coefficient can also be solved low, the present situation that growing-seedling period is long simultaneously.The inventive method is simple, with low cost, and Seeds of Dendrobium Candidum speed of emergence is fast, be applicable to Qin_Ba mountain areas and on the south region, and be convenient to promote, the needs of large area, scale breeding can be met completely.
Accompanying drawing explanation
Fig. 1 is that embodiment 1 dendrobium candidum sows the figure of the plant after 105 days.
Fig. 2 is that embodiment 1 dendrobium candidum sows the figure of the plant after 120 days.
Fig. 3 is that comparative example 1 bletilla sows the figure of the plant after 120 days.
Fig. 4 is that embodiment 2 dendrobium candidum sows the figure of the plant after 120 days.
Fig. 5 is that embodiment 3 dendrobium candidum sows the figure of the plant after 120 days.
Fig. 6 is that embodiment 4 dendrobium candidum sows the figure of the plant after 120 days.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in more detail, but protection scope of the present invention is not limited only to these embodiments.
Embodiment 1
1, the matrix after live bletilla is processed and sabot
Matrix after live for First Year bletilla is dried, cross 20 mesh sieves, then by the matrix after sieving and the bark powder of fermenting (being provided by Shaanxi Qin Feng agro-chemical companies) by volume 5:1 mix, again by gained mixture with carbendazim by volume for 1000:5 mixes, be mixed with the live matrix of dendrobium candidum, live for dendrobium candidum matrix being loaded bottom even is provided with in the rectangular dish (agricultural articles for use factory provides by Taizhou of Zhejiang skill source) that the length of side is 0.6cm diamond hole, the specification of rectangular dish is 54cm × 27cm × 6cm, be filled to 2/3 place of the rectangular dish degree of depth, flatten compacting, then rectangular dish is placed on the seedbed in plastic tunnel (in order to easy to operate, seedbed height is 1m, width is 2m, along seedbed length direction, baffle is set every the cuboid plank that 2m thickness is 3cm, then plastic paper is laid on seedbed, the surrounding of plastic paper is fixed on edge, seedbed, so that water), the water of the 2cm degree of depth is marked with in advance in seedbed, and water to matrix, matrix is thoroughly irrigated, can see that the saturated water surface is advisable with stromal surface.
The matrix of the live bletilla of above-mentioned First Year is that bark powder, Nutrition Soil, the river sand fermented for the mixture of 2:5:1 and the volume ratio of carbendazim are the mixture of 1000:1, is specifically shown in the record of embodiment 1 in CN103404341B by volume.
2, sowing and nursery
Seeds of Dendrobium Candidum is placed in 4 DEG C of refrigerator low temperature treatment 24 hours, then itself and talcum powder are fully mixed thoroughly for 1:10 in mass ratio, be poured on by the seed mixed on 60 object sieves, beat screen edges seed is evenly sowed on rectangular dish with waddy, sowing density is 3 ~ 5g/m
2, cover one deck preservative film in rectangular panel surface, water once after sowing one week every 3 days by the space between rectangular dish and rectangular dish, water upwards penetrates into matrix by the hole of rectangular tray bottom, need not take preservative film off and water.Seed just had sprouting phenomenon at about 20 ~ 25 days, was embodied in Seed imbibes and expanded, and had green appearance.
3, strong sprout
Treat that dendrobium candidum seedling grows two panels true leaf, spray weekly the methyl α-naphthyl acetate aqueous solution 1 time that quality-volumetric concentration is 0.001mg/L, sow after 40 days, a slice cotyledon is there is at protocorm top, sow about 55 ~ 60 days, grow the 1st true leaf at the another side that protocorm top cotyledon is corresponding, then spray weekly the methyl α-naphthyl acetate aqueous solution 1 time that quality-volumetric concentration is 0.001mg/L, and spray each 1 time of the di(2-ethylhexyl)phosphate amine aqueous solution that nutrient solutions and quality-volumetric concentration are 0.2g/L every 3 days.
Above-mentioned nutrient solution mixes the rear mixed liquor doubly obtained with distilled water diluting 10000 for 50:3:15 by macroelement by volume with micro-, molysite, and described macroelement is by 60.6g KNO
3, 40.83g KH
2pO
4, 12g NH
4nO
3, 19.8g (NH
4)
2sO
4, 14.7g, MgSO
47H
2o, 3.318g CaCl
2be settled to distilled water the mixed liquor that 1L obtains, described trace element is by 9.2745g H
3bO
3, 5.433g MnSO
4, 0.408g ZnCl
2, 0.749g CuSO
45H
2o, 0.1236g Na
2moO
4be settled to distilled water the mixed liquor that 1L obtains, described molysite is by 16.68g FeSO
47H
2o, 22.32g EDTA2Na distilled water is settled to the mixed liquor that 1L obtains.
Plant figure after the present embodiment Seeds of Dendrobium Candidum sows 105 days and 120 days is shown in Fig. 1 and Fig. 2 respectively.As seen from the figure, emerging better of dendrobium candidum, sow after 105 ~ 120 days, dendrobium candidum seedling grows 4 ~ 5 true leaves, and individual plants is comparatively large, and growing way is very fast, and robust plant, its lepisphere diameter stem can reach 1cm.
Comparative example 1
In embodiment 1, Seeds of Dendrobium Candidum is changed into bletilla seed, other steps are identical with embodiment 1.Fig. 3 is the plant figure of bletilla planting seed after 120 days, and as seen from the figure, matrix has moss occur, bletilla seed is not sprouted substantially.
Embodiment 2
Matrix after the live bletilla of process of the present embodiment in sabot step 1, matrix after live for First Year bletilla is dried, crosses 20 mesh sieves, then by the matrix after sieving and the bark powder of fermenting (being provided by Shaanxi Qin Feng agro-chemical companies) by volume 10:1 mix, again by gained mixture with carbendazim by volume for 1000:5 mixes, be mixed with the live matrix of dendrobium candidum, other steps are identical with embodiment 1.Plant figure after Seeds of Dendrobium Candidum sows 120 days is shown in Fig. 4.
Embodiment 3
In the present embodiment, the matrix of the live bletilla of First Year is that bark powder, Nutrition Soil, the river sand fermented for the mixture of 1:8:1 and the volume ratio of carbendazim are the mixture of 1000:1, is specifically shown in the record of embodiment 2 in CN103404341B by volume.Other steps are identical with embodiment 1, and the plant figure after Seeds of Dendrobium Candidum sows 120 days is shown in Fig. 5.
Embodiment 4
In the present embodiment, the matrix of the live bletilla of First Year is that bark powder, Nutrition Soil, the river sand fermented for the mixture of 8:10:1 and the volume ratio of carbendazim are the mixture of 1000:1, is specifically shown in the record of embodiment 2 in CN103404341B by volume.Other steps are identical with embodiment 1, and the plant figure after Seeds of Dendrobium Candidum sows 120 days is shown in Fig. 6.
Claims (4)
1. bletilla seedling medium recycles a method for live dendrobium candidum, it is characterized in that it comprises the steps composition:
(1) matrix after live bletilla is processed and sabot
Matrix after live for First Year bletilla is dried, sieved, then by the matrix after sieving and the bark powder of fermenting by volume 5 ~ 10:1 mix, gained mixture and carbendazim are by volume for 1000:5 mixes, be mixed with the live matrix of dendrobium candidum, live for dendrobium candidum matrix is loaded in the porose square plate in bottom, be filled to 2/3 place of the square plate degree of depth, flatten compacting, then square plate is placed on the seedbed in plastic tunnel, the water of 1 ~ 2cm degree of depth is marked with in advance in seedbed, water to matrix, matrix is thoroughly irrigated;
The matrix of the live bletilla of above-mentioned First Year is the bark powder, Nutrition Soil, the river sand that fermented be the mixture of 1 ~ 8:5 ~ 10:1 and the volume ratio of carbendazim is by volume the mixture of 1000:1;
(2) sowing and nursery
Seeds of Dendrobium Candidum is placed in 4 DEG C of refrigerator low temperature treatment 24 hours, then itself and talcum powder is fully mixed thoroughly for 1:10 in mass ratio, evenly sowed on square plate by the seed mixed, sowing density is 3 ~ 5g/m
2, at square plate surface coverage one deck preservative film, sow in one week and do not water, watered once every 3 days by the space between square plate and square plate later;
(3) strong sprout
Treat that dendrobium candidum seedling grows two panels true leaf, spray growth-promoting liquid 1 time weekly, and sprayed nutrient solutions and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution every 3 days;
The methyl α-naphthyl acetate aqueous solution of above-mentioned growth-promoting liquid to be quality-volumetric concentration be 0.0005 ~ 0.002mg/L; Nutrient solution mixes the rear mixed liquor doubly obtained with distilled water diluting 10000 for 50:3:15 by macroelement by volume with micro-, molysite, and described macroelement is by 60.6g KNO
3, 40.83g KH
2pO
4, 12g NH
4nO
3, 19.8g (NH
4)
2sO
4, 14.7g, MgSO
47H
2o, 3.318g CaCl
2be settled to distilled water the mixed liquor that 1L obtains, described trace element is by 9.2745g H
3bO
3, 5.433g MnSO
4, 0.408g ZnCl
2, 0.749gCuSO
45H
2o, 0.1236g Na
2moO
4be settled to distilled water the mixed liquor that 1L obtains, described molysite is by 16.68g FeSO
47H
2o, 22.32g EDTA2Na distilled water is settled to the mixed liquor that 1L obtains.
2. bletilla seedling medium according to claim 1 recycles the method for live dendrobium candidum, it is characterized in that: in described step (1), matrix after live for First Year bletilla is dried, sieve, then by the matrix after sieving and the bark powder of fermenting by volume 5:1 mix, gained mixture and carbendazim are by volume for 1000:5 mixes, be mixed with the live matrix of dendrobium candidum, the matrix of the live bletilla of wherein said First Year is the bark powder of fermenting, Nutrition Soil, river sand is by volume for the mixture of 2:5:1 and the volume ratio of carbendazim are the mixture of 1000:1.
3. bletilla seedling medium according to claim 1 recycles the method for live dendrobium candidum, it is characterized in that: in described step (3), the methyl α-naphthyl acetate aqueous solution of growth-promoting liquid to be quality-volumetric concentration be 0.001mg/L.
4. bletilla seedling medium according to claim 1 recycles the method for live dendrobium candidum, it is characterized in that: in described step (3), the quality-volumetric concentration of di(2-ethylhexyl)phosphate amine aqueous solution is 0.2g/L.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104370658A (en) * | 2014-11-13 | 2015-02-25 | 柳州市天姿园艺有限公司 | Nutrient solution capable of preventing and treating anthracnose of Dendrobium officinale |
| CN105340712B (en) * | 2015-12-03 | 2016-08-17 | 陕西师范大学 | A kind of greenhouse live propagation method of Herba Dendrobii |
| CN105359983A (en) * | 2015-12-24 | 2016-03-02 | 陕西师范大学 | Dendrobium officinale Kimura et Migo protocorm direct-seeding method |
| CN105948936B (en) * | 2016-06-01 | 2019-08-23 | 云南省热带作物科学研究所 | A kind of seedling medium suitable for torrid areas plant and preparation method thereof and purposes |
| CN106631606A (en) * | 2016-12-28 | 2017-05-10 | 广东国方医药科技有限公司 | Humic-acid-containing water-soluble fertilizer for dendrobium officinale as well as raw materials and preparation method of humic-acid-containing water-soluble fertilizer |
| CN110301303B (en) * | 2019-07-04 | 2021-11-26 | 广西壮族自治区农业科学院 | Dendrobium officinale cultivation bag and cultivation method of dendrobium officinale |
| CN113519551B (en) * | 2021-06-21 | 2022-08-05 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | Application of mixed nitrogen in promoting growth of rhizoma bletillae and improving photosynthetic efficiency and polysaccharide accumulation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270863A (en) * | 2013-04-27 | 2013-09-04 | 陕西师范大学 | Bletilla striata rapid propagation seedling cultivation method |
| CN103404341A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Direct seeding propagation method for Bletilla Striata in greenhouses |
| CN103404266A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Propagation method of bletilla striata |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270863A (en) * | 2013-04-27 | 2013-09-04 | 陕西师范大学 | Bletilla striata rapid propagation seedling cultivation method |
| CN103404341A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Direct seeding propagation method for Bletilla Striata in greenhouses |
| CN103404266A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Propagation method of bletilla striata |
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