CN109329016A - A kind of implantation methods of dendrobium candidum - Google Patents
A kind of implantation methods of dendrobium candidum Download PDFInfo
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- CN109329016A CN109329016A CN201811109941.3A CN201811109941A CN109329016A CN 109329016 A CN109329016 A CN 109329016A CN 201811109941 A CN201811109941 A CN 201811109941A CN 109329016 A CN109329016 A CN 109329016A
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- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention provides a kind of implantation methods of dendrobium candidum, and in particular to planting technical field.Planting matrix is packed into carrier of growing nonparasitically upon another plant by the present invention, and then dendrobium candidum seedling is inserted into filled matrix, watering and sprinkling nutrient solution, while keeping the illuminance of seedling and the water content of matrix, can harvest fresh stem after 6-9 months.Wherein, planting matrix includes following raw material: sawdust, tealeaf residue, peptone, beef extract, ethanol water, light-coloured vinegar and thiophanate methyl aqueous solution;Nutrient solution includes following raw material: plant ash, white sugar, brown sugar, eucalyptus leaves, Radix Notoginseng, gleditsia sinensis seed, aspirin, acetic acid, potassium hydroxide-ethanol solution and water.Dendrobium candidum of the present invention plantation high survival rate, seedling growth is vigorous, pest and disease damage incidence is low, crop cycle is short, fresh stem matter weight foot, fresh stem polyoses content are high after picking.
Description
[technical field]
The present invention relates to planting technical fields, and in particular to a kind of implantation methods of dendrobium candidum.
[background technique]
Dendrobium candidum is perennial orchid, is traditional valuable ingredient of traditional Chinese medicine, just there is the laudatory title of " help mesona " since ancient times,
Legendry is had much, is grown in the in the shade place of overhanging cliff throughout the year, first of Chinese nine big mesonas, polysaccharide component content is rich
Richness has enriching yin qi-restoratives, liver protection nourishing the stomach, inhibits a variety of important effects such as tumour, beauty treatment improving eyesight, anti-aging, strengthen immunity, long
Highest attention of the phase by people.Dendrobium candidum mainly originates in the ground such as China Yunnan-Guizhou, Zhejiang, Sichuan, Guangxi, Anhui, general raw
It is longer than the Perenniporia martius region of high temperature and humidity, likes the weather of warm and moist and the environment that yin-yang is fifty-fifty.Dendrobium candidum is
The very low epiphyte of a kind of slow growth, self-reproduction ability, requires growing environment high, can not resist cold, is not easy quickly to breed,
Such as sunlight or strong light direct beam too long and heavy rain, snow freeze can be at once dead.
Its medical value of dendrobium candidum is high, almost whole body is all precious, but wants due to its slow growth and to growing environment
Ask higher, wild low output, and through long-term artificial excavation, naturally wild resource is on the verge of exhaustion, is classified as focused protection by China
Medicinal material.Therefore, the artificial cultivation plantation of dendrobium candidum has become a kind of trend, becomes wild into family's kind, ensures dendrobium candidum money
The sustainable use in source, have become protection and develop this valuable ingredient of traditional Chinese medicine kind there is an urgent need to.Imitating for dendrobium candidum is wild
Plantation is the hot spot of current research, refers to the field grown mode of simulation dendrobium candidum, is grown nonparasitically upon another plant in other plant or certain
Growth reproduction on carrier is a kind of artificial method for planting that can obtain high-purity approximation wild varieties.China Patent Publication No. is
CN106332762A discloses a kind of side by dendrobium nobile plantation and crack of stone in a kind of entitled patent document of growing dendrobium mode
Method has saved cultivated land resource, and obtained dendrobium nobile also has higher nutritive value, but the planting matrix nutrition of dendrobium candidum is inadequate,
The fertilizer component used is again more single, causes plant strain growth small and weak, and pest and disease damage Infest rate is high, and plantation survival rate is low.Chinese patent
Publication number CN103636477A is disclosed in a kind of entitled patent document of the method for cultivation of dendrobium officinale a kind of in withered wood
The method that dendrobium candidum is planted on head, but the fertilising and harvesting of plant after plantation are not mentioned, therefore this method deposits plant after planting
Survival rate is low, problem that nutritive value is low.China Patent Publication No. CN104885891A plants iron sheet on a kind of entitled longan
The method of dendrobium nobile discloses a kind of method by dendrobium candidum kind in longan bark lobe ditch, and this method crop cycle is long, and
It is not strong to the prevention and treatment specific aim of fertilising and pest and disease damage, therefore plant growing way is weaker, survival rate is not high, nutritional ingredient is insufficient.
Plant that survival rate is low, slow growth, pest and disease damage are serious, nutritive value to solve dendrobium candidum seedling in the prior art
Low problem needs to develop a kind of implantation methods of dendrobium candidum, so that seedling percent is high, growth cycle is short, plant is effective
Active component content is high.
[summary of the invention]
Goal of the invention of the invention is: for dendrobium candidum seedling plantation survival rate, low, slow growth, pest and disease damage are tight
The low problem of weight, nutritive value, provides a kind of implantation methods of dendrobium candidum, and this method makes dendrobium candidum seedling plant survival rate
Height, growing way is vigorous, pest and disease damage incidence is low, crop cycle is short, and fresh stem matter weight foot, polyoses content are high after plant picking.
To achieve the goals above, The technical solution adopted by the invention is as follows:
A kind of implantation methods of dendrobium candidum, comprising the following steps:
A. it prepares planting matrix: by weight, taking 8-12 parts of sawdust, 3-7 parts of tealeaf residue, 3-5 parts of peptone and beef
1-3 parts of cream, it is 20-30 parts of 65%-75% ethanol water, 10-14 parts of light-coloured vinegar that volume fraction, which is then added, is stirred evenly,
It is heated boiling 60-80 minutes at 80-90 DEG C, is consequently cooled to room temperature, the methyl that mass fraction is 0.4%-0.6% is then added
2-4 parts of thiophanate aqueous solution, be sealed by fermentation 22-26 days to get;
B. it plants: taking planting matrix to be packed into carrier of growing nonparasitically upon another plant, then take the dendrobium candidum seedling 3-4 after hardening
Strain, after wrapping dendrobium candidum seedling root with moss, is inserted into filled planting matrix, then in insertion iron sheet stone
The planting matrix surface of dry measure used in former times seedling covers one layer of moss;
C. it waters: after covering moss, sprinkling profoundly water, then sprinkled profoundly water once, continue 4-6 times every 1-3 days, until iron sheet
Dendrobium nobile seedling grows sprouting, and a water was then poured every 1-2 days, and keeping the water content of matrix is 80%-84%;
D. spray nutrient solution: dendrobium candidum seedling plant after the 2-4 days, start spray nutrient solution;
E. culture harvesting: being carried out the culture of dendrobium candidum seedling by the method for step c and d, during which environment temperature is kept to exist
18-30 DEG C, intensity of illumination is in 8200-9800lx, and light application time was at 4-6 hours;Until obtained after 6-9 months dendrobium candidum at
Strain, can be harvested, every plant of plant can harvest fresh stem 9-11g;It is cut when harvesting and stays always tender, repeatedly, can continuously harvest 4-5
Year.
Optimization, sawdust described in step a is handled through 120-140 mesh screen sieving.
It advanced optimizes, carrier of growing nonparasitically upon another plant described in step b is any one in enclosure wall, persimmon tree and rotten wood
Or it is several;
The method of the enclosure wall filling planting matrix are as follows: be packed into planting matrix in enclosure wall gap;The enclosure wall gap
Width is 2-3cm, between each gap between be divided into 5-7cm;
The method of the persimmon tree filling planting matrix are as follows: select the treehole of persimmon tree or crotch to be packed into planting matrix,
?;Selected treehole or crotch are 0.6-2.2m apart from ground level.
The method of the rotten wood filling planting matrix are as follows: take rotten wood, rotting to dig a hole on wood, taking planting matrix
Matter is filled into hole;The hole depth is 1.4-1.8cm, hole dia 2-3cm, and the spacing in hole and hole is 5-7cm.
Further optimize, hardening matrix described in step b the preparation method comprises the following steps: by weight, taking coarse sand
0.5-1.5 parts, 0.5-1.5 parts of rubble powder, 1-3 parts of humus, 1-3 parts of pine bark, 1.4-1.6 parts of pomelo peel and pumpkin peel 0.8-
It 1.2 parts, stirs evenly, Yoghourt 0.6-0.8 parts, 0.2-0.4 parts of white sugar is then added, be uniformly mixed, be sealed by fermentation 20-24 days,
Fermentation soil, after then fermentation soil being taken to be laid on seedbed, uniform fold 0.5-1.5 portions of fermentation soil surface moss to get;Institute
Rubble powder is stated to handle through 80-100 mesh screen sieving.
Further optimize again, nutrient solution described in step d includes the raw material of following gravimetric method ratio: plant ash 0.5-
1.5 parts, 2-4 parts of white sugar, 0.8-1.2 parts of brown sugar, 1.2-1.6 parts of eucalyptus leaves, 0.8-1.2 parts of chinaberry leaf, 0.6-1 parts of Radix Notoginseng, soap
Seed 0.4-0.8 parts of angle, aspirin 1-3 piece, 0.5-1 parts of acetic acid, 12-16 parts of potassium hydroxide-ethanol solution and 18-22 parts of water;
The spray method of the nutrient solution are as follows: nutrient solution is taken, is stirred evenly, is then sprayed on the blade face of dendrobium candidum seedling, every
Sprinkling in 2-3 days is primary, usage amount 30-40ml/m2。
Further optimize again, the nutrient solution the preparation method comprises the following steps: by weight, taking gleditsia sinensis seed, eucalyptus leaves, chinaberry
Leaf, Radix Notoginseng, pulverize and sieve, and potassium hydroxide-ethanol solution is then added, is heated to reflux 40-50 minutes, connects at 60-70 DEG C
Filtering, collect filtrate I and filter residue;After taking filter residue that water is added again, heating is boiled with soft fire 50-60 minutes, is then filtered, is then received
Collect filtrate II;Merging filtrate I, filtrate II, then at 60-70 DEG C recycling ethyl alcohol to filtrate without alcohol taste, be subsequently added into acetic acid, stir
Uniformly, plant ash, white sugar, brown sugar, aspirin are eventually adding, is stirred evenly, and make white sugar, brown sugar dissolution to get;The hydrogen
Potassium oxide-ethanol solution is the preparation method comprises the following steps: by weight, take 4-6 parts of potassium hydroxide, addition volume fraction is 72%-
100-120 parts of 78% ethanol water, stirring and dissolving to get.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
1, then dendrobium candidum seedling is inserted into populated by the present invention using being packed into planting matrix in carrier of growing nonparasitically upon another plant
Matrix in, watering and sprinkling nutrient solution, while keeping the illuminance of seedling and the water content of matrix can harvest after 6-9 months
Fresh stem.Seedling growth of the present invention is vigorous, pest and disease damage incidence is low, and for plantation high survival rate up to 95%, crop cycle is short, fresh after picking
Stem matter weight foot, reaches 9-11g, fresh stem polyoses content is up to 40%.
2. the planting matrix that the present invention uses and carrier of growing nonparasitically upon another plant be with good compatibility, sawdust, tealeaf residue in matrix,
Peptone, beef extract make effective component be released and be enriched with by the reflux of ethanol water and the boiling of water, give iron sheet stone
The growth of dry measure used in former times seedling provides the ingredients such as nitrogen source abundant, minerals, carbohydrate, meanwhile, this process is also at effective sterilization
Reason process joined bacteriostasis antibiosis ingredient again after the boiling that flows back, so that matrix is had bactericidal and bacteriostatic effect for a long time, make dendrobium nobile seedling
It can grow up healthy and sound, effectively prevent disease, be used as matrix than single use sawdust, survival rate improves 17%, recovery ratio
Improve nearly 15%.
3. the present invention is by spraying nutrient solution to dendrobium candidum at regular time and quantity, it is ensured that dendrobium nobile is absorbed into sufficient nutriment, together
When, also it is unlikely to overnutrition occur and burn root.Nutrient solution prepares nutritional ingredient rich in raw material, wherein gleditsia sinensis
Mannosan and protein rich in seed, the carbonaceous organic materials such as flavones and saponins are abundant in Radix Notoginseng, through the boiling that flows back
Afterwards, ingredient therein is further dissolved out and is enriched with, meanwhile, the saponin constituent in gleditsia sinensis seed and Radix Notoginseng can have additional nutrients liquid in iron
The adhesive force on skin dendrobium nobile blade face extends the attachment time of nutrient solution, assimilation effect of the enhancing blade to nutrient in nutrient solution;Eucalyptus
The margosine in derived essential oil and chinaberry leaf in leaf has antibacterial insecticidal effect, is extracted using potassium hydroxide-ethanol solution
The antibacterial vermins-proof effect of nutrient solution is best, to primary pest snail, red spider, aphid of dendrobium candidum etc. have well prevention and treatment and
Killing effect, than having used the survival rate of plant for the nutrient solution for being not added with eucalyptus leaves and chinaberry leaf high, 20%, recovery ratio is improved
30%.
4. the dendrobium candidum seedling in the present invention will be planted again after hardening, in hardening matrix formulations therein
Humus, pine bark, pomelo peel, in pumpkin peel containing there are many micro members such as nitrogen source, the carbohydrate energy, phosphorus, potassium needed for seedling
Element makes these nutriments adequately be degraded after the fermentation maturity of Yoghourt and white sugar, is conducive to dendrobium candidum seedling
Absorption, greatly promote the adaptive capacity to environment and disease resistance ability of seedling during hardening.
5. the effect for planting dendrobium candidum in the present invention on persimmon tree is good, plant fresh weight and polyoses content are high.Persimmon tree
For wood living, wherein dendrobium candidum is taken root thereon throughout the access of conveying plant food, can share nutrition in persimmon tree at
Point;In addition, the environment of dendrobium candidum hobby warm moist, need to keep the long-term wet of plantation matrix, but the meeting again of excessive moisture
So that plant butt rot is led to death, the trunk of persimmon tree is tough and tensile corrosion-resistant, the coarse texture of bark and throughout carrying path
It can evacuate excessive moisture well, can be water-fast mould proof rotten, and the dense tree crown of persimmon tree can also shade sun-proof, be splendid attached
Raw tree species, by control plantation height, the survival rate and recovery ratio of dendrobium candidum are improved.
[specific embodiment]
Below with reference to embodiment, the invention will be further described.
Embodiment 1
A kind of implantation methods of dendrobium candidum, comprising the following steps:
A. it prepares planting matrix: by weight, taking 8 parts of sawdust, 3 parts of tealeaf residue, 3 parts of peptone and 1 part of beef extract, so
It is 20 parts of 65% ethanol water, 10 parts of light-coloured vinegar that volume fraction is added afterwards, stirs evenly, heats boiling 60 minutes at 80 DEG C,
It is consequently cooled to room temperature, it is 2 parts of thiophanate methyl aqueous solution of 0.4% that mass fraction, which is then added, is sealed by fermentation 22 days, i.e.,
?;
Wherein, sawdust is handled through 120 mesh screen sievings.
B. it plants: taking planting matrix to be packed into carrier of growing nonparasitically upon another plant, then take 3 plants of dendrobium candidum seedling after hardening,
It after wrapping dendrobium candidum seedling root with moss, is inserted into filled planting matrix, then in insertion dendrobium candidum
The planting matrix surface of seedling covers one layer of moss;
Wherein, carrier of growing nonparasitically upon another plant is enclosure wall;
The method of enclosure wall filling planting matrix are as follows: be packed into planting matrix in enclosure wall gap;The enclosure wall gap width
For 2cm, between each gap between be divided into 5cm;
Hardening matrix the preparation method comprises the following steps: by weight, take 0.5 part of coarse sand, 0.5 part of rubble powder, 1 part of humus,
1 part of pine bark, 1.4 parts of pomelo peel and 0.8 part of pumpkin peel, stir evenly, and 0.6 part of Yoghourt, 0.2 part of white sugar, mixing is then added
Uniformly, it is sealed by fermentation 20 days, obtains fermentation soil, after then fermentation soil being taken to be laid on seedbed, the uniform fold 0.5 on fermentation soil surface
Part moss to get;The rubble powder is handled through 80 mesh screen sievings.
C. it waters: after covering moss, sprinkling profoundly water, then sprinkled profoundly water once, continue 4 times every 1 day, until dendrobium candidum
Seedling grows sprouting, and a water was then poured every 1 day, and keeping the water content of matrix is 80%;
D. spray nutrient solution: dendrobium candidum seedling plant after the 2nd day, start spray nutrient solution;
Wherein, nutrient solution includes the raw material of following gravimetric method ratio: 0.5 part of plant ash, 2 parts of white sugar, 0.8 part of brown sugar, eucalyptus
1.2 parts of leaf, 0.8 part of chinaberry leaf, 0.6 part of Radix Notoginseng, 0.4 part of gleditsia sinensis seed, aspirin 1,0.5 part of acetic acid, potassium hydroxide-ethanol
12 parts and 18 parts of water of solution;The spray method of the nutrient solution are as follows: take nutrient solution, stir evenly, be then sprayed onto dendrobium candidum
It is primary every sprinkling in 2 days on the blade face of seedling, usage amount 30ml/m2。
Nutrient solution the preparation method comprises the following steps: by weight, taking gleditsia sinensis seed, eucalyptus leaves, chinaberry leaf, Radix Notoginseng, pulverizing and sieving, so
Potassium hydroxide-ethanol solution is added afterwards, is heated to reflux at 60 DEG C 40 minutes, then filters, collects filtrate I and filter residue;It takes again
After water is added in filter residue, heating is boiled with soft fire 50 minutes, is then filtered, and filtrate II is then collected;Merging filtrate I, filtrate II, then
At 60 DEG C recycle ethyl alcohol to filtrate without alcohol taste, be subsequently added into acetic acid, stir evenly, be eventually adding plant ash, white sugar, brown sugar, Ah
This woods, stirs evenly, and make white sugar, brown sugar dissolution to get;The potassium hydroxide-ethanol solution weighs the preparation method comprises the following steps: pressing
Measure part ratio, take 4 parts of potassium hydroxide, be added 100 parts of ethanol water that volume fraction is 72%, stirring and dissolving to get.
E. culture harvesting: being carried out the culture of dendrobium candidum seedling by the method for step c and d, during which environment temperature is kept to exist
18 DEG C, intensity of illumination is in 8200x, and light application time was at 4 hours;Until obtaining dendrobium candidum strain after 6 months, can be adopted
It receives.
Embodiment 2
A kind of implantation methods of dendrobium candidum, comprising the following steps:
A. it prepares planting matrix: by weight, taking 12 parts of sawdust, 7 parts of tealeaf residue, 5 parts of peptone and 3 parts of beef extract,
Then it is 30 parts of 75% ethanol water, 14 parts of light-coloured vinegar that volume fraction, which is added, is stirred evenly, and boiling 80 is heated at 90 DEG C and is divided
Clock is consequently cooled to room temperature, and 4 parts of thiophanate methyl aqueous solution that mass fraction is 0.6% are then added, are sealed by fermentation 26 days,
To obtain the final product;
Wherein, sawdust is handled through 140 mesh screen sievings.
B. it plants: taking planting matrix to be packed into carrier of growing nonparasitically upon another plant, then take 4 plants of dendrobium candidum seedling after hardening,
It after wrapping dendrobium candidum seedling root with moss, is inserted into filled planting matrix, then in insertion dendrobium candidum
The planting matrix surface of seedling covers one layer of moss;
Wherein, carrier of growing nonparasitically upon another plant is enclosure wall, persimmon tree and rotten wood;
The method of enclosure wall filling planting matrix are as follows: be packed into planting matrix in enclosure wall gap;The enclosure wall gap width
For 3cm, between each gap between be divided into 7cm;
The method of persimmon tree filling planting matrix are as follows: the treehole of persimmon tree or crotch is selected to be packed into planting matrix;
Selected treehole or crotch are 2.2m apart from ground level.
The method for wood filling planting matrix of rotting are as follows: take rotten wood, rotting to dig a hole on wood, planting matrix is taken to fill out
It is charged in hole;The hole depth is 1.8cm, hole dia 3cm, and the spacing in hole and hole is 7cm.
Hardening matrix the preparation method comprises the following steps: by weight, take 1.5 parts of coarse sand, 1.5 parts of rubble powder, 3 parts of humus,
3 parts of pine bark, 1.6 parts of pomelo peel and 1.2 parts of pumpkin peel, stir evenly, and 0.8 part of Yoghourt, 0.4 part of white sugar, mixing is then added
Uniformly, it is sealed by fermentation 24 days, obtains fermentation soil, after then fermentation soil being taken to be laid on seedbed, the uniform fold 1.5 on fermentation soil surface
Part moss to get;The rubble powder is handled through 100 mesh screen sievings.
C. it waters: after covering moss, sprinkling profoundly water, then sprinkled profoundly water once, continue 6 times every 3 days, until dendrobium candidum
Seedling grows sprouting, and a water was then poured every 2 days, and keeping the water content of matrix is 84%;
D. spray nutrient solution: dendrobium candidum seedling plant after the 4th day, start spray nutrient solution;
Wherein, nutrient solution includes the raw material of following gravimetric method ratio: 1.5 parts of plant ash, 4 parts of white sugar, 1.2 parts of brown sugar, eucalyptus
1.6 parts of leaf, 1.2 parts of chinaberry leaf, 1 part of Radix Notoginseng, 0.8 part of gleditsia sinensis seed, aspirin 3,1 part of acetic acid, potassium hydroxide-ethanol solution
16 parts and 22 parts of water;The spray method of the nutrient solution are as follows: take nutrient solution, stir evenly, be then sprayed onto dendrobium candidum seedling
Blade face on, spray once every 3 days, usage amount 40ml/m2。
Nutrient solution the preparation method comprises the following steps: by weight, taking gleditsia sinensis seed, eucalyptus leaves, chinaberry leaf, Radix Notoginseng, pulverizing and sieving, so
Potassium hydroxide-ethanol solution is added afterwards, is heated to reflux at 70 DEG C 50 minutes, then filters, collects filtrate I and filter residue;It takes again
After water is added in filter residue, heating is boiled with soft fire 60 minutes, is then filtered, and filtrate II is then collected;Merging filtrate I, filtrate II, then
At 70 DEG C recycle ethyl alcohol to filtrate without alcohol taste, be subsequently added into acetic acid, stir evenly, be eventually adding plant ash, white sugar, brown sugar, Ah
This woods, stirs evenly, and make white sugar, brown sugar dissolution to get;The potassium hydroxide-ethanol solution weighs the preparation method comprises the following steps: pressing
Measure part ratio, take 6 parts of potassium hydroxide, be added 120 parts of ethanol water that volume fraction is 78%, stirring and dissolving to get.
E. culture harvesting: being carried out the culture of dendrobium candidum seedling by the method for step c and d, during which environment temperature is kept to exist
30 DEG C, intensity of illumination is in 9800lx, and light application time was at 6 hours;Until obtaining dendrobium candidum strain after 9 months, can be adopted
It receives.
Embodiment 3
A kind of implantation methods of dendrobium candidum, comprising the following steps:
A. it prepares planting matrix: by weight, taking 9 parts of sawdust, 4 parts of tealeaf residue, 3.5 parts of peptone and beef extract 1.5
Part, it is 23 parts of 68% ethanol water, 11 parts of light-coloured vinegar that volume fraction, which is then added, is stirred evenly, and boiling 65 is heated at 82 DEG C
Minute, it is consequently cooled to room temperature, 2.5 parts of thiophanate methyl aqueous solution that mass fraction is 0.5% are then added, are sealed by fermentation 23
It to get;
Wherein, sawdust is handled through 125 mesh screen sievings.
B. it plants: taking planting matrix to be packed into carrier of growing nonparasitically upon another plant, then take 3 plants of dendrobium candidum seedling after hardening,
It after wrapping dendrobium candidum seedling root with moss, is inserted into filled planting matrix, then in insertion dendrobium candidum
The planting matrix surface of seedling covers one layer of moss;
Wherein, carrier of growing nonparasitically upon another plant is enclosure wall, persimmon tree and rotten wood;
The method of enclosure wall filling planting matrix are as follows: be packed into planting matrix in enclosure wall gap;The enclosure wall gap width
For 2.2cm, between each gap between be divided into 5.5cm;
The method of persimmon tree filling planting matrix are as follows: the treehole of persimmon tree or crotch is selected to be packed into planting matrix;
Selected treehole or crotch are 0.6m apart from ground level.
The method for wood filling planting matrix of rotting are as follows: take rotten wood, rotting to dig a hole on wood, planting matrix is taken to fill out
It is charged in hole;The hole depth is 1.4cm, hole dia 2cm, and the spacing in hole and hole is 5cm.
Hardening matrix the preparation method comprises the following steps: by weight, taking 0.8 part of coarse sand, 0.8 part of rubble powder, humus 1.5
Part, 1.5 parts of pine bark, 1.5 parts of pomelo peel and 0.9 part of pumpkin peel, stir evenly, and 0.7 part of Yoghourt, 0.3 part of white sugar is then added,
It is uniformly mixed, is sealed by fermentation 21 days, obtains fermentation soil, after then fermentation soil being taken to be laid on seedbed, uniformly covered on fermentation soil surface
0.8 portion of moss of lid to get;The rubble powder is handled through 85 mesh screen sievings.
C. it waters: after covering moss, sprinkling profoundly water, then sprinkled profoundly water once, continue 5 times every 2 days, until dendrobium candidum
Seedling grows sprouting, and a water was then poured every 1 day, and keeping the water content of matrix is 81%;
D. spray nutrient solution: dendrobium candidum seedling plant after the 3rd day, start spray nutrient solution;
Wherein, nutrient solution includes the raw material of following gravimetric method ratio: 0.8 part of plant ash, 2.5 parts of white sugar, 0.9 part of brown sugar, eucalyptus
1.3 parts of leaf, 0.9 part of chinaberry leaf, 0.7 part of Radix Notoginseng, 0.5 part of gleditsia sinensis seed, aspirin 1.5,0.6 part of acetic acid, potassium hydroxide-
13 parts and 19 parts of water of ethanol solution;The spray method of the nutrient solution are as follows: take nutrient solution, stir evenly, be then sprayed onto iron sheet
It on the blade face of dendrobium nobile seedling, sprays once every 3 days, usage amount 32ml/m2。
Nutrient solution the preparation method comprises the following steps: by weight, taking gleditsia sinensis seed, eucalyptus leaves, chinaberry leaf, Radix Notoginseng, pulverizing and sieving, so
Potassium hydroxide-ethanol solution is added afterwards, is heated to reflux at 62 DEG C 43 minutes, then filters, collects filtrate I and filter residue;It takes again
After water is added in filter residue, heating is boiled with soft fire 52 minutes, is then filtered, and filtrate II is then collected;Merging filtrate I, filtrate II, then
At 63 DEG C recycle ethyl alcohol to filtrate without alcohol taste, be subsequently added into acetic acid, stir evenly, be eventually adding plant ash, white sugar, brown sugar, Ah
This woods, stirs evenly, and make white sugar, brown sugar dissolution to get;The potassium hydroxide-ethanol solution weighs the preparation method comprises the following steps: pressing
Measure part ratio, take 4.5 parts of potassium hydroxide, be added 105 parts of ethanol water that volume fraction is 71%, stirring and dissolving to get.
E. culture harvesting: being carried out the culture of dendrobium candidum seedling by the method for step c and d, during which environment temperature is kept to exist
21 DEG C, intensity of illumination is in 8600lx, and light application time was at 4.5 hours;Until obtaining dendrobium candidum strain after 7 months, can carry out
Harvesting.
Embodiment 4
A kind of implantation methods of dendrobium candidum, comprising the following steps:
A. it prepares planting matrix: by weight, taking 11 parts of sawdust, 6 parts of tealeaf residue, 4.5 parts of peptone and beef extract 2.5
Part, it is 28 parts of 73% ethanol water, 13 parts of light-coloured vinegar that volume fraction, which is then added, is stirred evenly, and boiling 75 is heated at 88 DEG C
Minute, it is consequently cooled to room temperature, 3.5 parts of thiophanate methyl aqueous solution that mass fraction is 0.5% are then added, are sealed by fermentation 25
It to get;
Wherein, sawdust is handled through 135 mesh screen sievings.
B. it plants: taking planting matrix to be packed into carrier of growing nonparasitically upon another plant, then take 4 plants of dendrobium candidum seedling after hardening,
It after wrapping dendrobium candidum seedling root with moss, is inserted into filled planting matrix, then in insertion dendrobium candidum
The planting matrix surface of seedling covers one layer of moss;
Wherein, carrier of growing nonparasitically upon another plant is enclosure wall, persimmon tree and rotten wood;
The method of enclosure wall filling planting matrix are as follows: be packed into planting matrix in enclosure wall gap;The enclosure wall gap width
For 2.7cm, between each gap between be divided into 6.5cm;
The method of persimmon tree filling planting matrix are as follows: the treehole of persimmon tree or crotch is selected to be packed into planting matrix;
Selected treehole or crotch are 1m apart from ground level.
The method for wood filling planting matrix of rotting are as follows: take rotten wood, rotting to dig a hole on wood, planting matrix is taken to fill out
It is charged in hole;The hole depth is 1.7cm, hole dia 2.3cm, and the spacing in hole and hole is 5.3cm.
Hardening matrix the preparation method comprises the following steps: by weight, taking 1.2 parts of coarse sand, 1.3 parts of rubble powder, humus 2.5
Part, 2.5 parts of pine bark, 1.5 parts of pomelo peel and 1.1 parts of pumpkin peel, stir evenly, and 0.7 part of Yoghourt, 0.3 part of white sugar is then added,
It is uniformly mixed, is sealed by fermentation 23 days, obtains fermentation soil, after then fermentation soil being taken to be laid on seedbed, uniformly covered on fermentation soil surface
1.3 portions of mosses of lid to get;The rubble powder is handled through 95 mesh screen sievings.
C. it waters: after covering moss, sprinkling profoundly water, then sprinkled profoundly water once, continue 5 times every 2 days, until dendrobium candidum
Seedling grows sprouting, and a water was then poured every 2 days, and keeping the water content of matrix is 83%;
D. spray nutrient solution: dendrobium candidum seedling plant after the 3rd day, start spray nutrient solution;
Wherein, nutrient solution includes the raw material of following gravimetric method ratio: 1.2 parts of plant ash, 3.5 parts of white sugar, 1.1 parts of brown sugar, eucalyptus
1.5 parts of leaf, 1.1 parts of chinaberry leaf, 0.9 part of Radix Notoginseng, 0.7 part of gleditsia sinensis seed, aspirin 2.5,0.8 part of acetic acid, potassium hydroxide-
15 parts and 21 parts of water of ethanol solution;The spray method of the nutrient solution are as follows: take nutrient solution, stir evenly, be then sprayed onto iron sheet
It is primary every sprinkling in 2 days on the blade face of dendrobium nobile seedling, usage amount 35ml/m2。
Nutrient solution the preparation method comprises the following steps: by weight, taking gleditsia sinensis seed, eucalyptus leaves, chinaberry leaf, Radix Notoginseng, pulverizing and sieving, so
Potassium hydroxide-ethanol solution is added afterwards, is heated to reflux at 68 DEG C 47 minutes, then filters, collects filtrate I and filter residue;It takes again
After water is added in filter residue, heating is boiled with soft fire 58 minutes, is then filtered, and filtrate II is then collected;Merging filtrate I, filtrate II, then
At 67 DEG C recycle ethyl alcohol to filtrate without alcohol taste, be subsequently added into acetic acid, stir evenly, be eventually adding plant ash, white sugar, brown sugar, Ah
This woods, stirs evenly, and make white sugar, brown sugar dissolution to get;The potassium hydroxide-ethanol solution weighs the preparation method comprises the following steps: pressing
Part ratio is measured, takes 5.5 parts of potassium hydroxide, is added 115 parts of ethanol water that volume fraction is 72%-78%, stirring and dissolving, i.e.,
?.
E. culture harvesting: being carried out the culture of dendrobium candidum seedling by the method for step c and d, during which environment temperature is kept to exist
27 DEG C, intensity of illumination is in 9400lx, and light application time was at 5.5 hours;Until obtaining dendrobium candidum strain after 8 months, can carry out
Harvesting.
Embodiment 5
A kind of implantation methods of dendrobium candidum, comprising the following steps:
A. it prepares planting matrix: by weight, taking 10 parts of sawdust, 5 parts of tealeaf residue, 4 parts of peptone and 2 parts of beef extract,
Then it is 25 parts of 70% ethanol water, 12 parts of light-coloured vinegar that volume fraction, which is added, is stirred evenly, and boiling 70 is heated at 85 DEG C and is divided
Clock is consequently cooled to room temperature, and 3 parts of thiophanate methyl aqueous solution that mass fraction is 0.5% are then added, are sealed by fermentation 24 days,
To obtain the final product;
Wherein, sawdust is handled through 130 mesh screen sievings.
B. it plants: taking planting matrix to be packed into carrier of growing nonparasitically upon another plant, then take 4 plants of dendrobium candidum seedling after hardening,
It after wrapping dendrobium candidum seedling root with moss, is inserted into filled planting matrix, then in insertion dendrobium candidum
The planting matrix surface of seedling covers one layer of moss;
Wherein, carrier of growing nonparasitically upon another plant is enclosure wall, persimmon tree and rotten wood;
The method of enclosure wall filling planting matrix are as follows: be packed into planting matrix in enclosure wall gap;The enclosure wall gap width
For 2.5cm, between each gap between be divided into 6cm;
The method of persimmon tree filling planting matrix are as follows: the treehole of persimmon tree or crotch is selected to be packed into planting matrix;
Selected treehole or crotch are 1.4m apart from ground level.
The method for wood filling planting matrix of rotting are as follows: take rotten wood, rotting to dig a hole on wood, planting matrix is taken to fill out
It is charged in hole;The hole depth is 1.6cm, hole dia 2.5cm, and the spacing in hole and hole is 5.5cm.
Hardening matrix the preparation method comprises the following steps: by weight, taking 1 part of coarse sand, 1 part of rubble powder, 2 parts of humus, pine tree
2 parts of skin, 1.5 parts of pomelo peel and 1 part of pumpkin peel, stir evenly, and 0.7 part of Yoghourt, 0.3 part of white sugar is then added, and are uniformly mixed, close
Seal ferment 22 days, fermentation soil is obtained, after then fermentation soil being taken to be laid on seedbed, 1 portion of moss of uniform fold on fermentation soil surface, i.e.,
?;The rubble powder is handled through 90 mesh screen sievings.
C. it waters: after covering moss, sprinkling profoundly water, then sprinkled profoundly water once, continue 5 times every 2 days, until dendrobium candidum
Seedling grows sprouting, and a water was then poured every 2 days, and keeping the water content of matrix is 82%;
D. spray nutrient solution: dendrobium candidum seedling plant after the 3rd day, start spray nutrient solution;
Wherein, nutrient solution includes the raw material of following gravimetric method ratio: 1 part of plant ash, 3 parts of white sugar, 1 part of brown sugar, eucalyptus leaves 1.4
Part, 1 part of chinaberry leaf, 0.8 part of Radix Notoginseng, 0.6 part of gleditsia sinensis seed, aspirin 2,0.7 part of acetic acid, potassium hydroxide-ethanol solution 14
Part and 20 parts of water;The spray method of the nutrient solution are as follows: take nutrient solution, stir evenly, be then sprayed onto dendrobium candidum seedling
It is primary every sprinkling in 2 days on blade face, usage amount 35ml/m2。
Nutrient solution the preparation method comprises the following steps: by weight, taking gleditsia sinensis seed, eucalyptus leaves, chinaberry leaf, Radix Notoginseng, pulverizing and sieving, so
Potassium hydroxide-ethanol solution is added afterwards, is heated to reflux at 65 DEG C 45 minutes, then filters, collects filtrate I and filter residue;It takes again
After water is added in filter residue, heating is boiled with soft fire 55 minutes, is then filtered, and filtrate II is then collected;Merging filtrate I, filtrate II, then
At 65 DEG C recycle ethyl alcohol to filtrate without alcohol taste, be subsequently added into acetic acid, stir evenly, be eventually adding plant ash, white sugar, brown sugar, Ah
This woods, stirs evenly, and make white sugar, brown sugar dissolution to get;The potassium hydroxide-ethanol solution weighs the preparation method comprises the following steps: pressing
Measure part ratio, take 5 parts of potassium hydroxide, be added 110 parts of ethanol water that volume fraction is 74%, stirring and dissolving to get.
E. culture harvesting: being carried out the culture of dendrobium candidum seedling by the method for step c and d, during which environment temperature is kept to exist
24 DEG C, intensity of illumination is in 9000lx, and light application time was at 5 hours;Until obtaining dendrobium candidum strain after 8 months, can be adopted
It receives,.
Embodiment 6
Except in step a only with sawdust as planting matrix in addition to, remaining method is the same as embodiment 5.
Embodiment 7
In addition to the preparing raw material gleditsia sinensis seed and Radix Notoginseng is not used of nutrient solution in step d, remaining method is the same as embodiment 5.
Embodiment 8
In addition to the preparing raw material eucalyptus leaves and chinaberry leaf is not used of nutrient solution in step d, remaining method is the same as embodiment 5.
Embodiment 9
Except replacing potassium hydroxide-second by 74% ethanol water with volume fraction in the preparation method of step d nutrient solution
Outside alcoholic solution is flowed back, remaining method is the same as embodiment 5.
The comparison of 10 implementation result of embodiment
1. the preparation of laboratory sample: same a collection of 2700 plants of seedling of the dendrobium candidum multiplied under selection the same terms, random point
At 9 groups, every group 300 plants, experiment is finally obtained in the impartial plot in planting base 9 by the method plantation of embodiment 1-9 respectively
Sample 1-9.
2. evaluation method: to when harvesting, statistics plant survives quantity, calculates survival rate;And select leaf color vivid strong
The fresh stem for the dendrobium candidum strain that health, no disease and pests harm corrode is harvested, and counts the harvesting number of above-mentioned laboratory sample 1-9 respectively
Amount, stem length, stem thickness, fresh weight, recovery ratio, polyoses content calculate every average value.Finally with stem length, stem thickness, fresh weight, survive
Rate, recovery ratio, polyoses content average value, as evaluation index.
3. statistical result
It is counted according to above-mentioned evaluation method, obtains the Growth of Dendrobium candidum situation evaluation result of table 1.
1 Growth of Dendrobium candidum situation evaluation result of table
Remarks: recovery ratio %=harvesting quantity/survive quantity × 100%
4. experimental result
As shown in Table 1, the survival rate of plant of laboratory sample 1-5, recovery ratio and fresh stem quality are above laboratory sample 6-9.
For laboratory sample 6 only with sawdust as planting matrix, ingredient is single, makes seedling malnutrition, diseases and insect pests resistance
It is low, survival rate and harvesting stem quality are not only affected, healthy plant quantity is also affected, recovery ratio is caused to decline.
Nutrient solution in laboratory sample 7 has lacked two taste Chinese medicine of gleditsia sinensis seed and Radix Notoginseng, so that nutrient solution is to dendrobium candidum leaf
The adhesive force of piece declines, and affects the absorption of plant pair nutritional ingredient, leads to the quality decline of the fresh stem of strain, polyoses content compared with
It is low.
Nutrient solution in laboratory sample 8 has lacked two taste Chinese medicine of eucalyptus leaves and chinaberry leaf, so that dendrobium candidum plant is disease-resistant
Insect pest ability is remarkably decreased, and causes survival rate and recovery ratio extremely low, and fresh stem quality is also bad.
Refluxing extraction solvent of the volume fraction for 74% ethanol water as nutrient solution is used in laboratory sample 9, not
The antibacterial insecticidal constituent in eucalyptus leaves and chinaberry leaf can effectively be extracted so that survival rate of plant, especially recovery ratio under
Drop, the quality of the fresh stem of strain are also affected.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (6)
1. a kind of implantation methods of dendrobium candidum, which comprises the following steps:
A. it prepares planting matrix: by weight, taking 8-12 parts of sawdust, 3-7 parts of tealeaf residue, 3-5 parts of peptone and beef extract 1-3
Part, it is 20-30 parts of 65%-75% ethanol water, 10-14 parts of light-coloured vinegar that volume fraction, which is then added, is stirred evenly, in 80-90
It is heated boiling 60-80 minutes at DEG C, is consequently cooled to room temperature, the methyl Tobe that mass fraction is 0.4%-0.6% is then added
2-4 parts of saliva aqueous solution, be sealed by fermentation 22-26 days to get;
B. it plants: planting matrix being taken to be packed into carrier of growing nonparasitically upon another plant, then take 3-4 plants of dendrobium candidum seedling after hardening, use
It after moss wraps dendrobium candidum seedling root, is inserted into filled planting matrix, then in insertion dendrobium candidum children
The planting matrix surface of seedling covers one layer of moss;
C. it waters: after covering moss, sprinkling profoundly water, then sprinkled profoundly water once, continue 4-6 times every 1-3 days, until dendrobium candidum
Seedling grows sprouting, and a water was then poured every 1-2 days, and keeping the water content of matrix is 80%-84%;
D. spray nutrient solution: dendrobium candidum seedling plant after the 2-4 days, start spray nutrient solution;
E. culture harvesting: being carried out the culture of dendrobium candidum seedling by the method for step c and d, during which keeps environment temperature in 18-30
DEG C, intensity of illumination is in 8200-9800lx, and light application time was at 4-6 hours;Until obtaining dendrobium candidum strain after 6-9 months
It is harvested, every plant of plant can harvest fresh stem 9-11g.
2. a kind of implantation methods of dendrobium candidum according to claim 1, which is characterized in that the warp of sawdust described in step a
120-140 mesh screen sieving processing.
3. a kind of implantation methods of dendrobium candidum according to claim 1, which is characterized in that grow nonparasitically upon another plant described in step b
Carrier is any one or a few in enclosure wall, persimmon tree and rotten wood;
The method of the enclosure wall filling planting matrix are as follows: be packed into planting matrix in enclosure wall gap;The enclosure wall gap width
For 2-3cm, between each gap between be divided into 5-7cm;
The method of the persimmon tree filling planting matrix are as follows: the treehole of persimmon tree or crotch is selected to be packed into planting matrix;
Selected treehole or crotch are 0.6-2.2m apart from ground level.
The method of the rotten wood filling planting matrix are as follows: take rotten wood, rotting to dig a hole on wood, planting matrix is taken to fill out
It is charged in hole;The hole depth is 1.4-1.8cm, hole dia 2-3cm, and the spacing in hole and hole is 5-7cm.
4. a kind of implantation methods of dendrobium candidum according to claim 1, which is characterized in that hardening described in step b is used
Matrix the preparation method comprises the following steps: by weight, taking 0.5-1.5 parts of coarse sand, 0.5-1.5 parts of rubble powder, 1-3 parts of humus, pine tree
1-3 parts of skin, 1.4-1.6 parts of pomelo peel and 0.8-1.2 parts of pumpkin peel, stir evenly, and Yoghourt 0.6-0.8 parts, white sugar is then added
It 0.2-0.4 parts, is uniformly mixed, is sealed by fermentation 20-24 days, obtains fermentation soil, after then fermentation soil being taken to be laid on seedbed, fermenting
0.5-1.5 portions of mosses of native surface uniform fold to get;The rubble powder is handled through 80-100 mesh screen sieving.
5. a kind of implantation methods of dendrobium candidum according to claim 1, which is characterized in that nutrient solution described in step d
Raw material including following gravimetric method ratio: 0.5-1.5 parts of plant ash, 2-4 parts of white sugar, 0.8-1.2 parts of brown sugar, eucalyptus leaves 1.2-1.6
Part, 0.8-1.2 parts of chinaberry leaf, 0.6-1 parts of Radix Notoginseng, 0.4-0.8 parts of gleditsia sinensis seed, aspirin 1-3 piece, 0.5-1 parts of acetic acid, hydrogen-oxygen
Change potassium -12-16 parts of ethanol solution and water 18-22 parts;The spray method of the nutrient solution are as follows: take nutrient solution, stir evenly, then
It is sprayed on the blade face of dendrobium candidum seedling, usage amount 30-40ml/m primary every sprinkling in 2-3 days2。
6. a kind of implantation methods of dendrobium candidum according to claim 1 or 5, which is characterized in that the system of the nutrient solution
Preparation Method are as follows: by weight, take gleditsia sinensis seed, eucalyptus leaves, chinaberry leaf, Radix Notoginseng, pulverize and sieve, potassium hydroxide-second is then added
Alcoholic solution is heated to reflux 40-50 minutes at 60-70 DEG C, then filters, and collects filtrate I and filter residue;Take filter residue that water is added again
Afterwards, heating is boiled with soft fire 50-60 minutes, is then filtered, and filtrate II is then collected;Merging filtrate I, filtrate II, then 60-70 DEG C
Lower recycling ethyl alcohol, without alcohol taste, is subsequently added into acetic acid, stirs evenly to filtrate, is eventually adding plant ash, white sugar, brown sugar, A Si
Woods stirs evenly, and make white sugar, brown sugar dissolution to get;The potassium hydroxide-ethanol solution the preparation method comprises the following steps: by weight
Than, it takes 4-6 parts of potassium hydroxide, is added 100-120 parts of ethanol water that volume fraction is 72%-78%, stirring and dissolving, i.e.,
?.
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CN112640852B (en) * | 2020-12-27 | 2022-05-13 | 柳州市彭莫山农业科技有限公司 | Method for transporting insect tea larvae |
CN114303916A (en) * | 2021-12-31 | 2022-04-12 | 西南林业大学 | Organic planting method for dendrobium |
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