CN105104073A - Method for promoting none-flowering-phase kiwi fruits to flower under in vitro condition - Google Patents
Method for promoting none-flowering-phase kiwi fruits to flower under in vitro condition Download PDFInfo
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- CN105104073A CN105104073A CN201510520474.3A CN201510520474A CN105104073A CN 105104073 A CN105104073 A CN 105104073A CN 201510520474 A CN201510520474 A CN 201510520474A CN 105104073 A CN105104073 A CN 105104073A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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Abstract
The invention discloses a method for promoting none-flowering-phase kiwi fruits to flower under the in vitro condition. The method comprises the steps that certain chilling requirements are accumulated for in vitro twigs, an appropriate external environmental condition is given, the twigs are made to grow and develop in an established mode, and the purpose of generating flower buds and flowering ahead of time is achieved. Compared with the prior art, the method has the advantages that the none-flowering-phase kiwi fruits can obtain the flower buds, and the germplasm innovation for kiwi fruit haploid (double haploid) can be conducted multiple times in one year; the application scope is wide, the method can be popularized to other woody fruit trees, and original experimental materials are provided for the germplasm innovation based on the haploid (double haploid) technology.
Description
Technical field
The present invention relates to agricultural technology field, particularly relate to a kind of in vitro and promote that non-florescence kiwi fruit becomes the method for flower.
Background technology
Plant haploid (double haploid) technology is fast a kind of and effective germplasm innovation method, the method of cultivating by flower pesticide (microspore) realizes, be used for the economic crops such as Cruciferae, grass family and tobacco, but apply less in woody plant such as each fruit trees.Fruit tree is as a kind of woody plant, and its brephic (virgin phase) is longer, grows up and blooms once in latter 1 year; Can not realize planting in season more than a year as the herbaceous plant such as wheat and paddy rice and become flower.This seriously hinders the fruit tree germplasm innovation process based on monoploid (double haploid) technology.
At present, monoploid (double haploid) technology is not substantially carried out in Actinidia germplasm innovation.Existing 54 kinds of actinidia and 21 mutation, wherein actinidia eriantha constantly can budding between 1 year, bloom, result, all the other kiwi fruit kinds are if Chinese gooseberry and Kiwifruit are as the high cultivated species of market using value, also be the main object of breeding scholar germplasm innovation, florescence one is only once, even actinidia eriantha also can only obtain bud within its vegetative period, make the germplasm innovation cycle based on monoploid (double haploid) technology longer, compared with traditional crossbreeding, the superiority of monoploid (double haploid) germplasm innovation can not be embodied to greatest extent.
The physiological differentiation of kiwi fruit bud completes before surviving the winter.The branch completing bud physiological differentiation has possessed the genetic prerequisite forming bud, and namely related gene carries out selective expression.Give suitable condition in vitro branch, it can be made to sprout in advance according to its set development models, just can obtain bud, thus the bottleneck problem that solution monoploid (double haploid) technology is applied in fruit tree.
Summary of the invention
Goal of the invention: in order to overcome problems of the prior art, the present invention proposes one and kiwi fruit is buddingged in advance bloom, method is simple to operation, and a kind of in vitro that generalization is strong promotes that non-florescence kiwi fruit becomes the method for flower.
Technical scheme: in order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of in vitro promotes that non-florescence kiwi fruit becomes the method for flower, comprises the following steps:
(1) branch is selected and process: carry out November collecting the branch being used as cuttings; Gather annual lignified branch from the staminiferous plant that tree vigo(u)r is good, be placed in 0-4 DEG C of refrigerator cold-storage process with wet newspaper parcel;
(2) preparation of cutting bed: cutting bed is built in the green house of area without shade, bottom cutting bed, soil deep tillage is exposed to the sun and makes the furrow of wide 80-100cm, it lays the high cutting medium of 15-20cm, through being exposed to the sun and spraying and spray the effect that universal bactericide 25% Fluoxastrobin 1500 times of dilutions and 10% generation height 1000-1500 times of dilution reach sterilizing pesticide;
(3) cuttings prepares: taken out by the branch of step (1) chilling treatment, the cuttings cutting into 10-15cm is tied up, and lower cut aligns, and lower cut is immersed in rooting powder solution 10-12h, upper cut coats wound smears, prevents the invasion of damage by disease and insect and moisture from being lost;
(4) cuttage: the cuttage degree of depth is the 1/2-2/3 of cutting length; Water with watering can permeable after cuttage, cuttings is fully contacted with cutting bed cutting medium;
(5) management after cuttage: temperature controls at 20-25 DEG C; Cuttings do not sprout water the last week once permeable, take out the tip exhibition leaf after within 2-3 days, water once permeable; , top layer, seedbed sandy soil non-whitening hold agglomerating but not water outlet and be advisable generally to the requirement of moisture; Fine day need shade, and noon, temperature Gao Shixu lifted canopy ventilation;
(6) cuttings is sprouted and is buddingged and thin flower bud, and collection microspore is in monokaryotic stage and tests for monoploid (double haploid) germplasm innovation to the bud that double-core is early stage.
In described step (1), the chilling treatment time is not less than 1 week, carries out the accumulation of chilling requirement on the one hand, preserves on the one hand to isolated shoot.
More preferred, the cutting medium in described step (2) is that thin river sand, vermiculite and peat soil mix by the mass ratio of 2:1:2.
For obtaining more excellent rooting efficiency, with containing active ingredient being the rooting powder solution immersion cuttings lower cut that 20% methyl α-naphthyl acetate Dilution for powder 1000-2000 doubly configures.
More preferred, the selection of time of cuttage is before November to next year, kiwi fruit sprout in land for growing field crops sprouted then in described step (4).
Sufficient for ensureing the supply of flower bud development desired nutritional, the bud collection in described step (6) is specially young sprout 2-3cm pinching, and each cuttings retains 2-4 bud.
Further, the collection of described bud is selected to extract secondary flower bud.
Principle of the present invention is: after the physiological differentiation of kiwi fruit bud, namely the selective expression of related gene is completed, after isolated shoot completes the accumulation of certain chilling requirement, the external environmental condition be suitable for, it is made to grow according to set pattern growth, the object that reaching buddings in advance blooms.
Beneficial effect: a kind of in vitro provided by the invention promotes that non-florescence kiwi fruit becomes the method for flower, after isolated shoot being carried out the accumulation of certain chilling requirement, the external environmental condition be suitable for, makes it grow according to set pattern growth, the object that reaching buddings in advance blooms.Compared with prior art have the following advantages: a) make non-florescence kiwi fruit obtain bud, realize can repeatedly carrying out kiwi fruit monoploid (double haploid) germplasm innovation in 1 year; B) applied range, extends to other orchard fruit, for providing Initial experiments material based on the germplasm innovation of monoploid (double haploid) technology.
Accompanying drawing explanation
Fig. 1 is that the embodiment of the present invention 1 Chinese gooseberry in vitro promotes that the non-florescence becomes flower method schematic diagram.
Fig. 2 is that the embodiment of the present invention 2 Kiwifruit in vitro promotes that the non-florescence becomes flower method schematic diagram.
Fig. 3 is that the embodiment of the present invention 2 Kiwifruit in vitro promotes that the non-florescence becomes flower method schematic diagram.
Fig. 4 is that the embodiment of the present invention 3 actinidia eriantha in vitro promotes that the non-florescence becomes flower method schematic diagram.
Fig. 5 is that the embodiment of the present invention 3 actinidia eriantha in vitro promotes that the non-florescence becomes flower method schematic diagram.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail:
Embodiment 1:
1) test procedure:
At Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute Experimental Base, choose Chinese gooseberry branch and carry out isolated test, concrete a kind of in vitro promotes that non-florescence kiwi fruit becomes the method for flower, comprises the following steps:
(1) branch is selected and process: carry out November collecting the branch being used as cuttings; Gather annual lignified branch from the Chinese gooseberry staminiferous plant that tree vigo(u)r is good, be placed in 0-4 DEG C of refrigerator cold-storage process with wet newspaper parcel; The chilling treatment time is not less than 1 week, carries out the accumulation of chilling requirement on the one hand, preserves on the one hand to isolated shoot;
(2) preparation of cutting bed: cutting bed is built in the green house of area without shade, bottom cutting bed, soil deep tillage is exposed to the sun and makes the furrow of wide 80cm, it is laid the high cutting medium of 15cm, cutting medium is specially thin river sand, vermiculite and peat soil and mixes by the mass ratio of 2:1:2, through being exposed to the sun and spraying the effect that universal bactericide 25% Fluoxastrobin 1500 times of dilutions and 10% generation height 1000-1500 times of dilution reach sterilizing pesticide;
(3) cuttings prepares: taken out by the branch of step (1) chilling treatment, the cuttings cutting into 10cm is tied up, and lower cut aligns, and lower cut is immersed in rooting powder solution 10h, upper cut coats wound smears, prevents the invasion of damage by disease and insect and moisture from being lost;
(4) cuttage: in the selection of time November then of cuttage, the cuttage degree of depth is 1/2 of cutting length; Water with watering can permeable after cuttage, cuttings is fully contacted with cutting bed cutting medium;
(5) management after cuttage: temperature controls at 20-25 DEG C; Cuttings do not sprout water the last week once permeable, take out the tip exhibition leaf after within 2-3 days, water once permeable; , top layer, seedbed sandy soil non-whitening hold agglomerating but not water outlet and be advisable generally to the requirement of moisture; Fine day need shade, and noon, temperature Gao Shixu lifted canopy ventilation;
(6) cuttings is sprouted and is buddingged and thin flower bud, and collection microspore is in monokaryotic stage and tests for monoploid (double haploid) germplasm innovation to the bud that double-core is early stage; Sufficient for ensureing the supply of flower bud development desired nutritional, bud collection is specially young sprout 2-3cm pinching, and each cuttings retains 2-4 bud; Extract secondary flower bud.
2) result of the test:
After cuttage, 6-10 days sprouts sprout, and more than 90% cuttings can take out young sprout; Budding in advance and bloom, be used successfully to monoploid (double haploid) germplasm innovation; Percentage wherein containing bud young sprout is selected relevant with previous cuttings branch.
Embodiment 2:
1) test procedure:
At Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute Experimental Base, choose Kiwifruit branch and carry out isolated test, concrete a kind of in vitro promotes that non-florescence kiwi fruit becomes the method for flower, comprises the following steps:
(1) branch is selected and process: carry out November collecting the branch being used as cuttings; Gather annual lignified branch from the Kiwifruit staminiferous plant that tree vigo(u)r is good, be placed in 0-4 DEG C of refrigerator cold-storage process with wet newspaper parcel; The chilling treatment time is not less than 1 week, carries out the accumulation of chilling requirement on the one hand, preserves on the one hand to isolated shoot;
(2) preparation of cutting bed: cutting bed is built in the green house of area without shade, bottom cutting bed, soil deep tillage is exposed to the sun and makes the furrow of wide 100cm, it is laid the high cutting medium of 20cm, cutting medium is specially thin river sand, vermiculite and peat soil and mixes by the mass ratio of 2:1:2, through being exposed to the sun and spraying the effect that universal bactericide 25% Fluoxastrobin 1500 times of dilutions and 10% generation height 1000-1500 times of dilution reach sterilizing pesticide;
(3) cuttings prepares: taken out by the branch of step (1) chilling treatment, the cuttings cutting into 15cm is tied up, and lower cut aligns, and be immersed in rooting powder solution 12h, upper cut coats wound smears, prevents the invasion of damage by disease and insect and moisture from being lost;
(4) cuttage: in the selection of time November then of cuttage, the cuttage degree of depth is 2/3 of cutting length; Water with watering can permeable after cuttage, cuttings is fully contacted with cutting bed cutting medium;
(5) management after cuttage: temperature controls at 20-25 DEG C; Cuttings do not sprout water the last week once permeable, take out the tip exhibition leaf after within 2-3 days, water once permeable; , top layer, seedbed sandy soil non-whitening hold agglomerating but not water outlet and be advisable generally to the requirement of moisture; Fine day need shade, and noon, temperature Gao Shixu lifted canopy ventilation;
(6) cuttings is sprouted and is buddingged and thin flower bud, and collection microspore is in monokaryotic stage and tests for monoploid (double haploid) germplasm innovation to the bud that double-core is early stage; Sufficient for ensureing the supply of flower bud development desired nutritional, bud collection is specially young sprout 2-3cm pinching, and each cuttings retains 2-4 bud; Extract secondary flower bud.
2) result of the test:
After cuttage, 6-10 days sprouts sprout, and more than 90% cuttings can take out young sprout; Budding in advance and bloom, be used successfully to monoploid (double haploid) germplasm innovation; Percentage wherein containing bud young sprout is selected relevant with previous cuttings branch.
Embodiment 3:
1) test procedure:
At Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute Experimental Base, choose actinidia eriantha branch and carry out isolated test, concrete a kind of in vitro promotes that non-florescence kiwi fruit becomes the method for flower, comprises the following steps:
(1) branch is selected and process: carry out November collecting the branch being used as cuttings; Gather annual lignified branch from the actinidia eriantha staminiferous plant that tree vigo(u)r is good, be placed in 0-4 DEG C of refrigerator cold-storage process with wet newspaper parcel; The chilling treatment time is not less than 1 week, carries out the accumulation of chilling requirement on the one hand, preserves on the one hand to isolated shoot;
(2) preparation of cutting bed: cutting bed is built in the green house of area without shade, bottom cutting bed, soil deep tillage is exposed to the sun and makes the furrow of wide 90cm, it is laid the high cutting medium of 18cm, cutting medium is specially thin river sand, vermiculite and peat soil and mixes by the mass ratio of 2:1:2, through being exposed to the sun and spraying the effect that universal bactericide 25% Fluoxastrobin 1500 times of dilutions and 10% generation height 1000-1500 times of dilution reach sterilizing pesticide;
(3) cuttings prepares: taken out by the branch of step (1) chilling treatment, the cuttings cutting into 13cm is tied up, and lower cut aligns, and be immersed in rooting powder solution 11h, upper cut coats wound smears, prevents the invasion of damage by disease and insect and moisture from being lost;
(4) cuttage: in the selection of time November then of cuttage, the cuttage degree of depth is 1/2 of cutting length; Water with watering can permeable after cuttage, cuttings is fully contacted with cutting bed cutting medium;
(5) management after cuttage: temperature controls at 20-25 DEG C; Cuttings do not sprout water the last week once permeable, take out the tip exhibition leaf after within 2-3 days, water once permeable; , top layer, seedbed sandy soil non-whitening hold agglomerating but not water outlet and be advisable generally to the requirement of moisture; Fine day need shade, and noon, temperature Gao Shixu lifted canopy ventilation;
(6) cuttings is sprouted and is buddingged and thin flower bud, and collection microspore is in monokaryotic stage and tests for monoploid (double haploid) germplasm innovation to the bud that double-core is early stage; Sufficient for ensureing the supply of flower bud development desired nutritional, bud collection is specially young sprout 2-3cm pinching, and each cuttings retains 2-4 bud; Extract secondary flower bud.
2) result of the test:
After cuttage, 6-10 days sprouts sprout, and more than 90% cuttings can take out young sprout; Budding in advance and bloom, be used successfully to monoploid (double haploid) germplasm innovation; Percentage wherein containing bud young sprout is selected relevant with previous cuttings branch.
A kind of in vitro of the present invention promotes that non-florescence kiwi fruit becomes the method for flower, after isolated shoot being carried out the accumulation of certain chilling requirement, the external environmental condition be suitable for, it is made to grow according to set pattern growth, the object that reaching buddings in advance blooms, compared with prior art have the following advantages: a) make non-florescence kiwi fruit obtain bud, realize can repeatedly carrying out kiwi fruit monoploid (double haploid) germplasm innovation in 1 year; B) applied range, extends to other orchard fruit, for providing Initial experiments material based on the germplasm innovation of monoploid (double haploid) technology.
The present invention by the conditions such as manual control temperature, humidity make then November to next year land for growing field crops sprout all can carry out promoting that non-florescence kiwi fruit is buddingged in vitro before sprouting and bloom, realize several times a year buddingging blooming, for kiwi fruit monoploid (double haploid) germplasm innovation provides sufficient experiment starting material within the plant non-florescence.Method is simple, not high to facility requirements, can also promote the florescence restricted problem solving other plant and run in monoploid (double haploid) germplasm innovation.
Should be understood that, above embodiment is only not used in for illustration of the present invention and limits the scope of the invention, after having read the present invention, the amendment of those skilled in the art to the various equivalent form of value of the present invention has all fallen within the application's claims limited range.
Claims (7)
1. in vitro promotes that non-florescence kiwi fruit becomes a method for flower, it is characterized in that comprising the following steps:
(1) branch is selected and process: carry out November collecting the branch being used as cuttings; Gather annual lignified branch from the staminiferous plant that tree vigo(u)r is good, be placed in 0-4 DEG C of refrigerator cold-storage process with wet newspaper parcel;
(2) preparation of cutting bed: cutting bed is built in the green house of area without shade, bottom cutting bed, soil deep tillage is exposed to the sun and makes the furrow of wide 80-100cm, it lays the high cutting medium of 15-20cm, and be exposed to the sun 5-8h spray universal bactericide 25% Fluoxastrobin 1500 times of dilutions and 10% generation height 1000-1500 times of dilution;
(3) cuttings prepares: taken out by the branch of step (1) chilling treatment, the cuttings cutting into 10-15cm is tied up, and lower cut aligns, and lower cut is immersed in 10-12h in rooting powder solution, upper cut coats wound smears;
(4) cuttage: the cuttage degree of depth is the 1/2-2/3 of cutting length; Water with watering can permeable after cuttage, cuttings is fully contacted with cutting bed cutting medium;
(5) management after cuttage: temperature controls at 20-25 DEG C; Cuttings do not sprout water the last week once permeable, take out the tip exhibition leaf after within 2-3 days, water once permeable; , top layer, seedbed sandy soil non-whitening hold agglomerating but not water outlet and be advisable generally to the requirement of moisture; Fine day need shade, and noon, temperature Gao Shixu lifted canopy ventilation;
(6) cuttings is sprouted and is buddingged and thin flower bud, and collection microspore is in monokaryotic stage and tests for monoploid (double haploid) germplasm innovation to the bud that double-core is early stage.
2. in vitro according to claim 1 promotes that non-florescence kiwi fruit becomes the method for flower, it is characterized in that: in described step (1), the chilling treatment time is not less than 1 week.
3. in vitro according to claim 1 promotes that non-florescence kiwi fruit becomes the method for flower, it is characterized in that: the cutting medium in described step (2) is that thin river sand, vermiculite and peat soil mix by the mass ratio of 2:1:2.
4. in vitro according to claim 1 promotes that non-florescence kiwi fruit becomes the method for flower, it is characterized in that: the methyl α-naphthyl acetate Dilution for powder 1000-2000 of the active ingredient 20% that the rooting powder solution in described step (3) is produced by Sichuan Guoguang Agrochemical Co., Ltd. is doubly formulated.
5. in vitro according to claim 1 promotes that non-florescence kiwi fruit becomes the method for flower, it is characterized in that: in described step (4), the selection of time of cuttage is before November to next year, kiwi fruit sprout in land for growing field crops sprouted then.
6. in vitro according to claim 1 promotes that non-florescence kiwi fruit becomes the method for flower, and it is characterized in that: the bud collection in described step (6) is specially young sprout 2-3cm pinching, each cuttings retains 2-4 bud.
7. in vitro according to claim 5 promotes that non-florescence kiwi fruit becomes the method for flower, it is characterized in that: the collection of described bud is selected to extract secondary flower bud.
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| CN201510520474.3A CN105104073B (en) | 2015-08-21 | 2015-08-21 | A kind of in vitro promotes non-florescence Kiwi berry into colored method |
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| CN201510520474.3A CN105104073B (en) | 2015-08-21 | 2015-08-21 | A kind of in vitro promotes non-florescence Kiwi berry into colored method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109937724A (en) * | 2019-04-09 | 2019-06-28 | 大连润丰园珍稀果品开发有限公司 | A kind of anti-season mating system for tara vine |
| CN112042421A (en) * | 2020-09-08 | 2020-12-08 | 南通大学 | Method for promoting flowering of shrub willow |
-
2015
- 2015-08-21 CN CN201510520474.3A patent/CN105104073B/en active Active
Non-Patent Citations (2)
| Title |
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| 朱道圩: "河南省中华猕猴桃种质资源细胞分裂和染色体数目的研究", 《河南农学院院报》 * |
| 黄海琴等: "猕猴桃硬枝扦插繁殖技术", 《科技广场》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109937724A (en) * | 2019-04-09 | 2019-06-28 | 大连润丰园珍稀果品开发有限公司 | A kind of anti-season mating system for tara vine |
| CN112042421A (en) * | 2020-09-08 | 2020-12-08 | 南通大学 | Method for promoting flowering of shrub willow |
| CN112042421B (en) * | 2020-09-08 | 2022-04-01 | 南通大学 | Method for promoting flowering of shrub willow |
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