CN105104073B - A kind of in vitro promotes non-florescence Kiwi berry into colored method - Google Patents
A kind of in vitro promotes non-florescence Kiwi berry into colored method Download PDFInfo
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- CN105104073B CN105104073B CN201510520474.3A CN201510520474A CN105104073B CN 105104073 B CN105104073 B CN 105104073B CN 201510520474 A CN201510520474 A CN 201510520474A CN 105104073 B CN105104073 B CN 105104073B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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Abstract
The invention discloses a kind of in vitro to promote non-florescence Kiwi berry into colored method, after the accumulation that isolated shoot is carried out to certain chilling requirement, give suitable external environmental condition, it is set to be developed according to set pattern growth, reach the purpose buddingged bloom in advance, compared with prior art with advantages below：A) non-florescence Kiwi berry is caused to obtain bud, Kiwi berry monoploid (dihaploid) germplasm innovation can repeatedly be carried out by realizing 1 year；B) have a wide range of application, extend to other orchard fruits, Initial experiments material is provided for the germplasm innovation based on monoploid (dihaploid) technology.
Description
Technical field
The present invention relates to agricultural technology field, more particularly to a kind of in vitro to promote non-florescence Kiwi berry into colored side
Method.
Background technology
Plant haploid (dihaploid) technology is a kind of rapidly and effectively germplasm innovation method, can be (small by flower pesticide
Spore) method of culture realizes, it is used for the industrial crops such as Cruciferae, grass family and tobacco, but such as each in xylophyta
Using less in fruit trees.Fruit tree is as a kind of xylophyta, and its brephic (juvenile phase) is longer, and latter year of growing up blooms once；
Season plantation more than a year can not be realized as the herbaceous plant such as wheat and rice and into flower.This serious obstruction is (double based on monoploid
Monoploid) technology fruit tree germplasm innovation process.
At present, monoploid (dihaploid) technology is not carried out substantially in Actinidia germplasm innovation.Actinidia shows
There are 54 kinds and 21 mutation, wherein actinidia eriantha can constantly budding between 1 year, bloom, result, and remaining Kiwi berry kind is such as
Chinese gooseberry and the Kiwifruit cultigen high as market application value, and breeding scholar's germplasm innovation are main
Object, 1 year florescence only once, even actinidia eriantha also can only obtain bud within its growth period so that based on monoploid
The germplasm innovation cycle of (dihaploid) technology is longer, compared with traditional crossbreeding, it is impossible to embody monoploid to greatest extent
The superiority of (dihaploid) germplasm innovation.
The Physiological Differentiation of macaque peach bud is completed before surviving the winter.The branch for completing bud Physiological Differentiation has possessed to form bud
Genetic prerequisite, i.e. related gene carried out selective expression.Give suitable condition in vitro branch, can make its according to it both
Determine development models to sprout in advance, bud can be obtained, so as to solve the bottle that monoploid (dihaploid) technology is applied in fruit tree
Neck problem.
The content of the invention
Goal of the invention：In order to overcome problems of the prior art, the present invention proposes one kind so that Kiwi berry shifts to an earlier date
Budding and bloom, method is simple to operation, and a kind of strong in vitro of generalization promotes non-florescence Kiwi berry into colored method.
Technical scheme：In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：A kind of in vitro
Non- florescence Kiwi berry is promoted to comprise the following steps into colored method：
(1) branch selection and processing：It is collected the branch for being used as cuttings November；Gathered on the staminiferous plant good from tree vigo(u)r
The branch of annual lignifying, it is placed in 0-4 DEG C of refrigerator cold-storage with wet newspaper parcel and handles；
(2) preparation of cutting bed：Cutting bed is built in the warmhouse booth of area without shade, and cutting bed bottom soil deep tillage is exposed to the sun simultaneously
Wide 80-100cm furrow are made, the high cutting mediums of 15-20cm are laid thereon, through being exposed to the sun and spraying and spray universal bactericide
25% 1500 times of Fluoxastrobin dilution and the high 1000-1500 times of dilution of 10% generation have the function that sterilizing pesticide；
(3) cuttings prepares：The branch of step (1) chilling treatment is taken out, the cuttings for cutting into 10-15cm is tied up, incision
Mouth alignment, and lower cut is immersed in rooting powder solution 10-12h, upper cut coats wound smears, prevents the invasion of pest and disease damage
It is lost with moisture；
(4) cuttage：Cuttage depth is the 1/2-2/3 of cutting length；Poured with watering can permeable after cuttage, make cuttings and cutting bed
Cutting medium fully contacts；
(5) management after cuttage：Temperature control is at 20-25 DEG C；Cuttings do not sprout the last week pour it is permeable once, take out tip lamina
Pour within 2-3 days afterwards it is permeable once；Requirement generally to moisture is seedbed top layer sandy soil non-whitening, holds agglomerating but not water outlet and is
Preferably；Fine day needs to shade, and noon temperature Gao Shixu lifts canopy ventilation；
(6) cuttings, which is sprouted, buddings and dredges flower bud, and collection microspore is in monokaryotic stage to the bud of double-core early stage and is used for monoploid
(dihaploid) germplasm innovation is tested.
In the step (1) the chilling treatment time be not less than 1 week, on the one hand progress chilling requirement accumulation, on the one hand to from
Body branch is preserved.
It is more highly preferred to, the cutting medium in the step (2) is that thin river sand, vermiculite and peat soil press 2:1:2 quality
Ratio mixes.
It is that 20% 1000-2000 times of methyl α-naphthyl acetate Dilution for powder configures with containing active ingredient to obtain more excellent rooting efficiency
The rooting powder solution immersion cuttings lower cut formed.
It is more highly preferred to, the selection of time of cuttage November to next year crop field Kiwi berry sprout then in the step (4)
Before sprouting.
To ensure that nutrition supply is sufficient needed for flower bud development, the bud collection in the step (6) is specially young sprout 2-3cm
Pinching, each cuttings retain 2-4 bud.
Further, secondary flower bud is extractd in the collection selection of the bud.
The present invention principle be：After the Physiological Differentiation of macaque peach bud, that is, the selective expression of related gene is completed, in vitro
After branch completes certain chilling requirement accumulation, suitable external environmental condition is given, it is developed according to set pattern growth, reaches
To the purpose bloomed of buddingging in advance.
Beneficial effect：A kind of in vitro provided by the invention promotes non-florescence Kiwi berry into colored method, will be in vitro
After branch carries out the accumulation of certain chilling requirement, suitable external environmental condition is given, it is developed according to set pattern growth,
Reach the purpose buddingged bloom in advance.There is advantages below compared with prior art：A) non-florescence Kiwi berry is caused to obtain bud,
Kiwi berry monoploid (dihaploid) germplasm innovation can repeatedly be carried out by realizing 1 year；B) have a wide range of application, extend to other wood
This fruit tree, Initial experiments material is provided for the germplasm innovation based on monoploid (dihaploid) technology.
Brief description of the drawings
Fig. 1 is that the Chinese gooseberry in vitro of the embodiment of the present invention 1 promotes the non-florescence into flower method schematic diagram.
Fig. 2 is that the Kiwifruit in vitro of the embodiment of the present invention 2 promotes the non-florescence into flower method schematic diagram.
Fig. 3 is that the Kiwifruit in vitro of the embodiment of the present invention 2 promotes the non-florescence into flower method schematic diagram.
Fig. 4 is that the actinidia eriantha in vitro of the embodiment of the present invention 3 promotes the non-florescence into flower method schematic diagram.
Fig. 5 is that the actinidia eriantha in vitro of the embodiment of the present invention 3 promotes the non-florescence into flower method schematic diagram.
Embodiment
With reference to embodiment, the present invention is described in further detail：
Embodiment 1：
1) test procedure：
In Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute Experimental Base, choose Chinese gooseberry branch and tried in vitro
Test, a kind of specific in vitro promotes non-florescence Kiwi berry to comprise the following steps into colored method：
(1) branch selection and processing：It is collected the branch for being used as cuttings November；The Chinese gooseberry good from tree vigo(u)r
The branch of annual lignifying is gathered on staminiferous plant, being placed in 0-4 DEG C of refrigerator cold-storage with wet newspaper parcel is handled；The chilling treatment time is not
Less than 1 week, the accumulation of chilling requirement is on the one hand carried out, on the one hand isolated shoot is preserved；
(2) preparation of cutting bed：Cutting bed is built in the warmhouse booth of area without shade, and cutting bed bottom soil deep tillage is exposed to the sun simultaneously
Wide 80cm furrow are made, lay the high cutting mediums of 15cm thereon, cutting medium is specially thin river sand, vermiculite and peat soil by 2:
1:2 mass ratio mixes, through being exposed to the sun and spraying 25% Fluoxastrobin of universal bactericide, 1500 times of dilutions and 10% generation
High 1000-1500 times of dilution has the function that sterilizing pesticide；
(3) cuttings prepares：The branch of step (1) chilling treatment is taken out, the cuttings for cutting into 10cm is tied up, lower cut pair
Together, and by lower cut rooting powder solution 10h is immersed in, upper cut coats wound smears, prevents invasion and the moisture of pest and disease damage
It is lost；
(4) cuttage：November, cuttage depth are the 1/2 of cutting length to the selection of time of cuttage then；Watering can is used after cuttage
Pour permeable, cuttings is fully contacted with cutting bed cutting medium；
(5) management after cuttage：Temperature control is at 20-25 DEG C；Cuttings do not sprout the last week pour it is permeable once, take out tip lamina
Pour within 2-3 days afterwards it is permeable once；Requirement generally to moisture is seedbed top layer sandy soil non-whitening, holds agglomerating but not water outlet and is
Preferably；Fine day needs to shade, and noon temperature Gao Shixu lifts canopy ventilation；
(6) cuttings, which is sprouted, buddings and dredges flower bud, and collection microspore is in monokaryotic stage to the bud of double-core early stage and is used for monoploid
(dihaploid) germplasm innovation is tested；To ensure that nutrition supply is sufficient needed for flower bud development, bud collection is specially young sprout 2-3cm
Pinching, each cuttings retain 2-4 bud；Extract secondary flower bud.
2) result of the test：
6-10 days sprouts sprout after cuttage, and more than 90% cuttings can take out young sprout；Budding in advance and bloom, be used successfully to monoploid
(dihaploid) germplasm innovation；Wherein the percentage of the young sprout containing bud is relevant with previous cuttings branch selection.
Embodiment 2：
1) test procedure：
In Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute Experimental Base, choose Kiwifruit branch and tried in vitro
Test, a kind of specific in vitro promotes non-florescence Kiwi berry to comprise the following steps into colored method：
(1) branch selection and processing：It is collected the branch for being used as cuttings November；The Kiwifruit good from tree vigo(u)r
The branch of annual lignifying is gathered on staminiferous plant, being placed in 0-4 DEG C of refrigerator cold-storage with wet newspaper parcel is handled；The chilling treatment time is not
Less than 1 week, the accumulation of chilling requirement is on the one hand carried out, on the one hand isolated shoot is preserved；
(2) preparation of cutting bed：Cutting bed is built in the warmhouse booth of area without shade, and cutting bed bottom soil deep tillage is exposed to the sun simultaneously
Wide 100cm furrow are made, lay the high cutting mediums of 20cm thereon, cutting medium is specially that thin river sand, vermiculite and peat soil are pressed
2:1:2 mass ratio mixes, through being exposed to the sun and spraying 25% Fluoxastrobin of universal bactericide, 1500 times of dilutions and 10%
The high 1000-1500 times of dilution of generation has the function that sterilizing pesticide；
(3) cuttings prepares：The branch of step (1) chilling treatment is taken out, the cuttings for cutting into 15cm is tied up, lower cut pair
Together, rooting powder solution 12h is immersed in, upper cut coats wound smears, prevents that the invasion of pest and disease damage and moisture from being lost；
(4) cuttage：November, cuttage depth are the 2/3 of cutting length to the selection of time of cuttage then；Watering can is used after cuttage
Pour permeable, cuttings is fully contacted with cutting bed cutting medium；
(5) management after cuttage：Temperature control is at 20-25 DEG C；Cuttings do not sprout the last week pour it is permeable once, take out tip lamina
Pour within 2-3 days afterwards it is permeable once；Requirement generally to moisture is seedbed top layer sandy soil non-whitening, holds agglomerating but not water outlet and is
Preferably；Fine day needs to shade, and noon temperature Gao Shixu lifts canopy ventilation；
(6) cuttings, which is sprouted, buddings and dredges flower bud, and collection microspore is in monokaryotic stage to the bud of double-core early stage and is used for monoploid
(dihaploid) germplasm innovation is tested；To ensure that nutrition supply is sufficient needed for flower bud development, bud collection is specially young sprout 2-3cm
Pinching, each cuttings retain 2-4 bud；Extract secondary flower bud.
2) result of the test：
6-10 days sprouts sprout after cuttage, and more than 90% cuttings can take out young sprout；Budding in advance and bloom, be used successfully to monoploid
(dihaploid) germplasm innovation；Wherein the percentage of the young sprout containing bud is relevant with previous cuttings branch selection.
Embodiment 3：
1) test procedure：
In Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute Experimental Base, choose actinidia eriantha branch and tried in vitro
Test, a kind of specific in vitro promotes non-florescence Kiwi berry to comprise the following steps into colored method：
(1) branch selection and processing：It is collected the branch for being used as cuttings November；The actinidia eriantha good from tree vigo(u)r
The branch of annual lignifying is gathered on staminiferous plant, being placed in 0-4 DEG C of refrigerator cold-storage with wet newspaper parcel is handled；The chilling treatment time is not
Less than 1 week, the accumulation of chilling requirement is on the one hand carried out, on the one hand isolated shoot is preserved；
(2) preparation of cutting bed：Cutting bed is built in the warmhouse booth of area without shade, and cutting bed bottom soil deep tillage is exposed to the sun simultaneously
Wide 90cm furrow are made, lay the high cutting mediums of 18cm thereon, cutting medium is specially thin river sand, vermiculite and peat soil by 2:
1:2 mass ratio mixes, through being exposed to the sun and spraying 25% Fluoxastrobin of universal bactericide, 1500 times of dilutions and 10% generation
High 1000-1500 times of dilution has the function that sterilizing pesticide；
(3) cuttings prepares：The branch of step (1) chilling treatment is taken out, the cuttings for cutting into 13cm is tied up, lower cut pair
Together, rooting powder solution 11h is immersed in, upper cut coats wound smears, prevents that the invasion of pest and disease damage and moisture from being lost；
(4) cuttage：November, cuttage depth are the 1/2 of cutting length to the selection of time of cuttage then；Watering can is used after cuttage
Pour permeable, cuttings is fully contacted with cutting bed cutting medium；
(5) management after cuttage：Temperature control is at 20-25 DEG C；Cuttings do not sprout the last week pour it is permeable once, take out tip lamina
Pour within 2-3 days afterwards it is permeable once；Requirement generally to moisture is seedbed top layer sandy soil non-whitening, holds agglomerating but not water outlet and is
Preferably；Fine day needs to shade, and noon temperature Gao Shixu lifts canopy ventilation；
(6) cuttings, which is sprouted, buddings and dredges flower bud, and collection microspore is in monokaryotic stage to the bud of double-core early stage and is used for monoploid
(dihaploid) germplasm innovation is tested；To ensure that nutrition supply is sufficient needed for flower bud development, bud collection is specially young sprout 2-3cm
Pinching, each cuttings retain 2-4 bud；Extract secondary flower bud.
2) result of the test：
6-10 days sprouts sprout after cuttage, and more than 90% cuttings can take out young sprout；Budding in advance and bloom, be used successfully to monoploid
(dihaploid) germplasm innovation；Wherein the percentage of the young sprout containing bud is relevant with previous cuttings branch selection.
A kind of in vitro of the present invention promotes non-florescence Kiwi berry necessarily to be needed isolated shoot cold into colored method
After the accumulation of amount, suitable external environmental condition is given, it is developed according to set pattern growth, reaches to budding in advance and bloom
Purpose, compared with prior art with advantages below：A) non-florescence Kiwi berry is caused to obtain bud, realizing 1 year repeatedly to enter
Row Kiwi berry monoploid (dihaploid) germplasm innovation；B) have a wide range of application, extend to other orchard fruits, for based on single times
The germplasm innovation of body (dihaploid) technology provides Initial experiments material.
The present invention by the conditions such as manual control temperature, humidity before November to next year crop field sprout sprouts then
Non- florescence Kiwi berry can be promoted to budding in vitro to bloom, realize several times a year to budding within the plant non-florescence and bloom, be macaque
Peach monoploid (dihaploid) germplasm innovation provides sufficient experiment original material.Method is simple, not high to facility requirements, may be used also
To promote the florescence restricted problem for solving other plant and being run into monoploid (dihaploid) germplasm innovation.
It should be pointed out that above embodiment is only illustrative of the invention and is not intended to limit the scope of the invention,
After having read the present invention, modification of the those skilled in the art to the various equivalent form of values of the present invention falls within power appended by the application
Profit requires limited range.
Claims (7)
1. a kind of in vitro promotes non-florescence Kiwi berry into colored method, it is characterised in that comprises the following steps：
（1）Branch selects and processing：It is collected the branch for being used as cuttings November；Gathered 1 year on the staminiferous plant good from tree vigo(u)r
The branch of raw lignifying, it is placed in 0-4 DEG C of refrigerator cold-storage with wet newspaper parcel and handles；
（2）The preparation of cutting bed：Cutting bed is built in the warmhouse booth of area without shade, and cutting bed bottom soil deep tillage is exposed to the sun and made
Wide 80-100cm furrow, the high cutting mediums of 15-20cm are laid thereon, be exposed to the sun and 5-8h and spray 25% phonetic bacterium of broad-spectrum germicide
1500 times of dilutions of ester and the high 1000-1500 times of dilution of 10% generation；
（3）Cuttings prepares：By step（1）The branch of chilling treatment is taken out, and the cuttings for cutting into 10-15cm is tied up, lower cut pair
Together, lower cut is immersed in 10-12h in rooting powder solution, upper cut coats wound smears；
（4）Cuttage：Cuttage depth is the 1/2-2/3 of cutting length；Poured with watering can permeable after cuttage, make cuttings and cutting bed skewer
Slotting matrix fully contacts；
（5）Management after cuttage：Temperature control is at 20-25 DEG C；Cuttings do not sprout the last week pour it is permeable once, take out 2- after tip lamina
Pour within 3 days it is permeable once；Requirement generally to moisture is seedbed top layer sandy soil non-whitening, holds agglomerating but not water outlet；Fine day needs
Shade, noon temperature Gao Shixu lift canopy ventilation；
（6）Cuttings, which is sprouted, buddings and dredges flower bud, and collection microspore is in monokaryotic stage to the bud of double-core early stage and is used for monoploid or double
The experiment of times body germplasm innovation.
2. in vitro according to claim 1 promotes non-florescence Kiwi berry into colored method, it is characterised in that：It is described
Step（1）The middle chilling treatment time is not less than 1 week.
3. in vitro according to claim 1 promotes non-florescence Kiwi berry into colored method, it is characterised in that：It is described
Step（2）In cutting medium for thin river sand, vermiculite and peat soil press 2:1:2 mass ratio mixes.
4. in vitro according to claim 1 promotes non-florescence Kiwi berry into colored method, it is characterised in that：It is described
Step（3）In the methyl α-naphthyl acetate pulvis of active ingredient 20% that is produced by Sichuan Guoguang Agrochemical Co., Ltd. of rooting powder solution
1000-2000 times of dilution is formulated.
5. in vitro according to claim 1 promotes non-florescence Kiwi berry into colored method, it is characterised in that：It is described
Step（4）The selection of time of middle cuttage is before November to next year crop field Kiwi berry sprout sprouts then.
6. in vitro according to claim 1 promotes non-florescence Kiwi berry into colored method, it is characterised in that：It is described
Step（6）In bud collection be specially young sprout 2-3cm pinching, each cuttings retains 4 buds of 2-.
7. in vitro according to claim 5 promotes non-florescence Kiwi berry into colored method, it is characterised in that：It is described
Secondary flower bud is extractd in the collection selection of bud.
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| CN109937724A (en) * | 2019-04-09 | 2019-06-28 | 大连润丰园珍稀果品开发有限公司 | A kind of anti-season mating system for tara vine |
| CN112042421B (en) * | 2020-09-08 | 2022-04-01 | 南通大学 | Method for promoting flowering of shrub willow |
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Non-Patent Citations (2)
| Title |
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| 河南省中华猕猴桃种质资源细胞分裂和染色体数目的研究;朱道圩;《河南农学院院报》;19821231(第1期);第47页 * |
| 猕猴桃硬枝扦插繁殖技术;黄海琴等;《科技广场》;20121231(第12期);第176-177页 * |
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