CN105051189B - 具有改善特性的酰基-acp还原酶 - Google Patents

具有改善特性的酰基-acp还原酶 Download PDF

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Publication number
CN105051189B
CN105051189B CN201480004992.3A CN201480004992A CN105051189B CN 105051189 B CN105051189 B CN 105051189B CN 201480004992 A CN201480004992 A CN 201480004992A CN 105051189 B CN105051189 B CN 105051189B
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China
Prior art keywords
fatty
aar
fatty alcohol
seq
polypeptide
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Chinese (zh)
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CN105051189A (zh
Inventor
M·鲁德
N·特里安
A·席尔默
J·加诺
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Genomatica Inc
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REG Life Sciences LLC
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Priority to CN201910169609.4A priority Critical patent/CN109897834A/zh
Priority to CN201910693927.0A priority patent/CN110387352A/zh
Publication of CN105051189A publication Critical patent/CN105051189A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0108—Long-chain acyl-[acyl-carrier-protein] reductase (1.2.1.80)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
CN201480004992.3A 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶 Active CN105051189B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201910169609.4A CN109897834A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910693927.0A CN110387352A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361753273P 2013-01-16 2013-01-16
US61/753,273 2013-01-16
PCT/US2014/011859 WO2014113571A2 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN201910693927.0A Division CN110387352A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910169609.4A Division CN109897834A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶

Publications (2)

Publication Number Publication Date
CN105051189A CN105051189A (zh) 2015-11-11
CN105051189B true CN105051189B (zh) 2019-08-06

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CN201480004992.3A Active CN105051189B (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910169609.4A Pending CN109897834A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910693927.0A Pending CN110387352A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶

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CN201910169609.4A Pending CN109897834A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910693927.0A Pending CN110387352A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶

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US (6) US9683219B2 (enExample)
EP (3) EP2946009B1 (enExample)
JP (5) JP6063585B2 (enExample)
KR (4) KR101746040B1 (enExample)
CN (3) CN105051189B (enExample)
AU (3) AU2014207522B2 (enExample)
BR (1) BR112015017005A2 (enExample)
CA (2) CA3143642A1 (enExample)
CO (1) CO7461134A2 (enExample)
ES (2) ES2600354T3 (enExample)
MX (2) MX356997B (enExample)
MY (1) MY167434A (enExample)
WO (1) WO2014113571A2 (enExample)

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EP2882843A4 (en) 2012-08-10 2016-04-06 Opx Biotechnologies Inc MICRO-ORGANISMS AND METHOD FOR THE PRODUCTION OF FATTY ACIDS AND PRODUCTS DERIVED FROM FATTY ACIDS
KR101746040B1 (ko) 2013-01-16 2017-06-13 알이지 라이프 사이언시스, 엘엘씨 개선된 특성들을 갖는 아실-ａｃｐ 레덕타아제
US20150057465A1 (en) 2013-03-15 2015-02-26 Opx Biotechnologies, Inc. Control of growth-induction-production phases
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN111748511A (zh) * 2014-07-18 2020-10-09 基因组股份公司 微生物的脂肪二醇产生
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
BR112019020784A2 (pt) 2017-04-03 2020-04-28 Genomatica, Inc. variantes de tioesterase com atividade melhorada para a produção de derivados de ácidos graxos de cadeia média
EP3844269A4 (en) * 2018-08-31 2022-07-27 Genomatica, Inc. PRODUCTION OF NON-NATIVE MONOUNSATURATED FATTY ACIDS IN BACTERIA
WO2020047304A1 (en) 2018-08-31 2020-03-05 Genomatica, Inc. Xylr mutant for improved xylose utilization or improved co-utilization of glucose and xylose
US12649937B2 (en) 2021-03-29 2026-06-09 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
WO2023178193A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

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WO2011006137A2 (en) * 2009-07-09 2011-01-13 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
CN102695799A (zh) * 2009-09-27 2012-09-26 Opx生物工艺学公司 用于制备3-羟基丙酸和其它产物的方法
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US9873865B2 (en) 2018-01-23
AU2017200596A1 (en) 2017-02-23
AU2014207522B2 (en) 2016-12-15
CA2898317A1 (en) 2014-07-24
US20170342388A1 (en) 2017-11-30
JP6063585B2 (ja) 2017-01-18
MX2015009138A (es) 2015-11-06
JP6211162B2 (ja) 2017-10-11
KR20150105471A (ko) 2015-09-16
JP2021006067A (ja) 2021-01-21
CN110387352A (zh) 2019-10-29
JP7094343B2 (ja) 2022-07-01
EP3385375B1 (en) 2021-06-16
KR102265148B1 (ko) 2021-06-15
CO7461134A2 (es) 2015-11-30
US10208294B2 (en) 2019-02-19
MX356997B (es) 2018-06-22
EP3103867A1 (en) 2016-12-14
KR20170066694A (ko) 2017-06-14
AU2017200596B2 (en) 2019-01-24
KR102082247B1 (ko) 2020-02-28
US20150361454A1 (en) 2015-12-17
JP2017042170A (ja) 2017-03-02
AU2014207522A1 (en) 2015-05-07
KR101746040B1 (ko) 2017-06-13
BR112015017005A2 (pt) 2017-08-15
CN109897834A (zh) 2019-06-18
MY167434A (en) 2018-08-28
EP2946009B1 (en) 2016-07-27
WO2014113571A3 (en) 2014-11-13
ES2600354T3 (es) 2017-02-08
US12173329B2 (en) 2024-12-24
JP6523393B2 (ja) 2019-05-29
US20240117324A1 (en) 2024-04-11
KR20200020998A (ko) 2020-02-26
MX346830B (es) 2017-04-03
CA2898317C (en) 2022-03-01
JP2016503663A (ja) 2016-02-08
JP2017209118A (ja) 2017-11-30
US20220090028A1 (en) 2022-03-24
US20160348080A1 (en) 2016-12-01
US9683219B2 (en) 2017-06-20
WO2014113571A2 (en) 2014-07-24
EP3103867B1 (en) 2018-05-16
AU2019202790A1 (en) 2019-05-16
JP6926143B2 (ja) 2021-08-25
ES2675224T3 (es) 2018-07-09
EP2946009A2 (en) 2015-11-25
KR20210072147A (ko) 2021-06-16
JP2019141083A (ja) 2019-08-29
US11130944B2 (en) 2021-09-28
CA3143642A1 (en) 2014-07-24
CN105051189A (zh) 2015-11-11
EP3385375A1 (en) 2018-10-10
US20200010813A1 (en) 2020-01-09

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Patentee after: Genomatica, Inc.

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Patentee before: REG Life Sciences Co.,Ltd.