AU2014207522B2 - Acyl-ACP reductase with improved properties - Google Patents

Acyl-ACP reductase with improved properties Download PDF

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Publication number
AU2014207522B2
AU2014207522B2 AU2014207522A AU2014207522A AU2014207522B2 AU 2014207522 B2 AU2014207522 B2 AU 2014207522B2 AU 2014207522 A AU2014207522 A AU 2014207522A AU 2014207522 A AU2014207522 A AU 2014207522A AU 2014207522 B2 AU2014207522 B2 AU 2014207522B2
Authority
AU
Australia
Prior art keywords
mutation
fatty
fatty alcohol
seq
aar
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2014207522A
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English (en)
Other versions
AU2014207522A1 (en
Inventor
Jacob GANO
Mathew Rude
Andreas Schirmer
Na Trinh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genomatica Inc
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Publication of AU2014207522A1 publication Critical patent/AU2014207522A1/en
Assigned to LS9, INC. reassignment LS9, INC. Amend patent request/document other than specification (104) Assignors: GANO, Jacob, LS9, INC., RUDE, MATHEW, SCHIRMER, ANDREAS, TRINH, NA
Assigned to REG Life Sciences, LLC reassignment REG Life Sciences, LLC Request for Assignment Assignors: LS9, INC.
Application granted granted Critical
Publication of AU2014207522B2 publication Critical patent/AU2014207522B2/en
Priority to AU2017200596A priority Critical patent/AU2017200596B2/en
Priority to AU2019202790A priority patent/AU2019202790A1/en
Assigned to GENOMATICA, INC. reassignment GENOMATICA, INC. Request for Assignment Assignors: REG Life Sciences, LLC
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0108—Long-chain acyl-[acyl-carrier-protein] reductase (1.2.1.80)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
AU2014207522A 2013-01-16 2014-01-16 Acyl-ACP reductase with improved properties Ceased AU2014207522B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2017200596A AU2017200596B2 (en) 2013-01-16 2017-01-30 Acyl-ACP reductase with improved properties
AU2019202790A AU2019202790A1 (en) 2013-01-16 2019-04-18 Acyl-ACP reductase with improved properties

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361753273P 2013-01-16 2013-01-16
US61/753,273 2013-01-16
PCT/US2014/011859 WO2014113571A2 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2017200596A Division AU2017200596B2 (en) 2013-01-16 2017-01-30 Acyl-ACP reductase with improved properties

Publications (2)

Publication Number Publication Date
AU2014207522A1 AU2014207522A1 (en) 2015-05-07
AU2014207522B2 true AU2014207522B2 (en) 2016-12-15

Family

ID=50473752

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2014207522A Ceased AU2014207522B2 (en) 2013-01-16 2014-01-16 Acyl-ACP reductase with improved properties
AU2017200596A Ceased AU2017200596B2 (en) 2013-01-16 2017-01-30 Acyl-ACP reductase with improved properties
AU2019202790A Abandoned AU2019202790A1 (en) 2013-01-16 2019-04-18 Acyl-ACP reductase with improved properties

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2017200596A Ceased AU2017200596B2 (en) 2013-01-16 2017-01-30 Acyl-ACP reductase with improved properties
AU2019202790A Abandoned AU2019202790A1 (en) 2013-01-16 2019-04-18 Acyl-ACP reductase with improved properties

Country Status (13)

Country Link
US (6) US9683219B2 (enExample)
EP (3) EP2946009B1 (enExample)
JP (5) JP6063585B2 (enExample)
KR (4) KR20210072147A (enExample)
CN (3) CN109897834A (enExample)
AU (3) AU2014207522B2 (enExample)
BR (1) BR112015017005A2 (enExample)
CA (2) CA2898317C (enExample)
CO (1) CO7461134A2 (enExample)
ES (2) ES2600354T3 (enExample)
MX (2) MX356997B (enExample)
MY (1) MY167434A (enExample)
WO (1) WO2014113571A2 (enExample)

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CN109182238A (zh) 2012-08-10 2019-01-11 嘉吉有限公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
WO2014113571A2 (en) * 2013-01-16 2014-07-24 Ls9, Inc. Acyl-acp reductase with improved properties
WO2014146026A1 (en) 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction of chemicals
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
JP6603658B2 (ja) 2013-07-19 2019-11-06 カーギル インコーポレイテッド 脂肪酸及び脂肪酸誘導体の製造のための微生物及び方法
BR112017001105A2 (pt) * 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
CN111094558A (zh) 2017-04-03 2020-05-01 基因组股份公司 对产生中链脂肪酸衍生物具有改善的活性的硫酯酶变体
US12098168B2 (en) 2018-08-31 2024-09-24 Genomatica, Inc. XylR mutant for improved xylose utilization or improved co-utilization of glucose and xylose preliminary
EP3844269A4 (en) * 2018-08-31 2022-07-27 Genomatica, Inc. PRODUCTION OF NON-NATIVE MONOUNSATURATED FATTY ACIDS IN BACTERIA
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Citations (1)

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Also Published As

Publication number Publication date
JP2017042170A (ja) 2017-03-02
US20200010813A1 (en) 2020-01-09
AU2019202790A1 (en) 2019-05-16
KR20210072147A (ko) 2021-06-16
CA2898317A1 (en) 2014-07-24
JP2021006067A (ja) 2021-01-21
US20240117324A1 (en) 2024-04-11
US20170342388A1 (en) 2017-11-30
CA2898317C (en) 2022-03-01
EP3103867B1 (en) 2018-05-16
ES2675224T3 (es) 2018-07-09
CN105051189A (zh) 2015-11-11
JP7094343B2 (ja) 2022-07-01
US20220090028A1 (en) 2022-03-24
KR20170066694A (ko) 2017-06-14
BR112015017005A2 (pt) 2017-08-15
CN109897834A (zh) 2019-06-18
KR20150105471A (ko) 2015-09-16
KR101746040B1 (ko) 2017-06-13
US9683219B2 (en) 2017-06-20
WO2014113571A2 (en) 2014-07-24
JP2019141083A (ja) 2019-08-29
MY167434A (en) 2018-08-28
MX2015009138A (es) 2015-11-06
CN110387352A (zh) 2019-10-29
JP2016503663A (ja) 2016-02-08
WO2014113571A3 (en) 2014-11-13
AU2014207522A1 (en) 2015-05-07
US20160348080A1 (en) 2016-12-01
EP3385375A1 (en) 2018-10-10
EP2946009A2 (en) 2015-11-25
US20150361454A1 (en) 2015-12-17
KR102082247B1 (ko) 2020-02-28
AU2017200596A1 (en) 2017-02-23
MX356997B (es) 2018-06-22
EP3385375B1 (en) 2021-06-16
US11130944B2 (en) 2021-09-28
KR102265148B1 (ko) 2021-06-15
CA3143642A1 (en) 2014-07-24
JP6523393B2 (ja) 2019-05-29
US12173329B2 (en) 2024-12-24
CN105051189B (zh) 2019-08-06
US10208294B2 (en) 2019-02-19
AU2017200596B2 (en) 2019-01-24
JP2017209118A (ja) 2017-11-30
KR20200020998A (ko) 2020-02-26
US9873865B2 (en) 2018-01-23
ES2600354T3 (es) 2017-02-08
EP3103867A1 (en) 2016-12-14
JP6063585B2 (ja) 2017-01-18
JP6926143B2 (ja) 2021-08-25
CO7461134A2 (es) 2015-11-30
MX346830B (es) 2017-04-03
EP2946009B1 (en) 2016-07-27
JP6211162B2 (ja) 2017-10-11

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Owner name: REG LIFE SCIENCES, LLC

Free format text: FORMER APPLICANT(S): LS9, INC.

FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: GENOMATICA, INC.

Free format text: FORMER OWNER(S): REG LIFE SCIENCES, LLC

MK14 Patent ceased section 143(a) (annual fees not paid) or expired