CN104770225B - A kind of vitro grafting method from root anvil apple sprout - Google Patents
A kind of vitro grafting method from root anvil apple sprout Download PDFInfo
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- CN104770225B CN104770225B CN201510205556.9A CN201510205556A CN104770225B CN 104770225 B CN104770225 B CN 104770225B CN 201510205556 A CN201510205556 A CN 201510205556A CN 104770225 B CN104770225 B CN 104770225B
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses a kind of vitro grafting method from root anvil apple sprout, specifically implement according to following steps：Preparation before grafting：Including building platform, grafting place is carried out disinfection；Choose stock to be grafted and scion；The nursery stock of selection is carried out into vitro grafting.Present invention facilitates advantageously forming for callus, plant percent is substantially increased；Worked by grafting storehouse, substantially increase grafting efficiency and quality, saved labour costs and reduce the activity duration.The stock adopted from field and scion can be preserved in freezer, substantially increase the flexibility of grafting time；The nursery stock for grafting can for a long time preserve after dipping in wax in freezer, and can long-distance transportation, improve the output of nursery stock, promote the Multiplication and extension of high quality seedling.
Description
Technical field
The invention belongs to fruit-tree grafting technical field, it is related to a kind of vitro grafting method from root anvil apple sprout.
Background technology
At present, most domestic Apple breeding method carries out apple using the method that simple bud connects (also referred to as " T " shaped budding)
Grafting.Laizhou, Shandong uses plate budding instead since the end of the eighties in last century, because it is without band xylem, extends grafting time, right
Economize on resources, rapidly expand planting scale played great role, the survival rate and growing way of above-mentioned engrafting method are above tradition
Simple bud connection.But, this engrafting method is generally field budding, due to being operated in open-air atmosphere, weather and temperature is subject to unavoidably
Influence, have impact on grafting efficiency and survival rate, and running cost is high, be unfavorable for producing in enormous quantities.
The content of the invention
It is an object of the invention to provide a kind of vitro grafting method from root anvil apple sprout, existing field grafting is solved
Method exist grafting efficiency is low and the low problem of survival rate.
The technical solution adopted in the present invention is,
A kind of vitro grafting method from root anvil apple sprout, specifically implements according to following steps：
Step 1：Preparation before grafting：Including building platform, grafting place is carried out disinfection；
Step 2：Choose stock to be grafted and scion；
Step 3：Seedling grafting：The nursery stock that will be chosen through step 2 carries out vitro grafting.
The features of the present invention is also resided in,
The specific implementation step of step 1 is：
Step 1.1：Workbench prepares：Using scaffold building platform, and face laying woodwork plate on the table；In wood
A baffle plate perpendicular with woodwork plate is fixed at work plate plane 42cm, as the standard of graft stock length；
Step 1.2：Grafting place is carried out disinfection：It is 3%~5% 84 thimerosals to grafting place to use mass concentration
Spray disinfectant is carried out, 24~36h of door and window is closed after sprinkling；
In step 1, the length of workbench is 2.3~2.5m, and width is 0.8~1.2m, is highly 0.6~0.8m.
The specific implementation step of step 2 is：
Step 2.1：The pretreatment of stock：0.6~1.0mm of roots oxidizing, the stock of 8~12mm of limb diameter are chosen, first
Stock is carried out into cutting back pretreatment at 45~50cm, then enters refrigeration house storage, storage requirement is：Temperature is 0~2 DEG C, humidity
It is not less than 90%；
Step 2.2：Scion is harvested：Scion is harvested, collecting time is 2 months mid or late November to next year early and middle ten dayses,
It is required that being carried out after soil freezes；Scion-seed tree requirement has high yield, stable yields, the proterties of high-quality, and stalwartness of growing, without inspection
Epidemic disease object；It is elected to be the branch of scion, it is desirable to which the substantial healthy and strong, sprout of growth is full, no flower bud, size is matched with graft stock；
Step 2.3：Scion is preserved：The scion that will be harvested through step 2.2, first carries out defoliation treatment, removes all blades, so
Bundling and it is put into polybag, refrigeration house storage afterwards, storage temperature is -2~2 DEG C, humidity is 90~95%.
The specific implementation step of step 3 is：
Step 3.1：Grafting knife and scissors are sterilized with liquor potassic permanganate；
Step 3.2：Cut stock：By the plate washer of the adjacent workbench in the root of the stock chosen through step 2, mouth selection will be cut
At the top of stock at the sprout of work edge of table, stock and fixed length are cut；Cut inclined-plane：Carried on the back from the stock sprout of fixed length
Face, knife is entered at 1.4~1.6cm of sprout lower section, cuts out the smooth bevel of 2~4cm of length；Switchback：In stock bevel by upper
At 1/4~1/3 down, along stock inclined-plane toward 1~2cm of incision longitudinal incision, and time partial application 1.4~1.6cm of inclined-plane at,
Switchback length is the half of chamfer length；
Step 3.3：Cut scion：Cut at the 0.8~1.2cm of terminal bud top of the scion that will be chosen through step 2, it is desirable to protect
Card scion has 2~4 full buds, and the length of each full bud is 5~6cm；
Step 3.4：Stock and the docking of scion：The scion processed by the stock processed through step 3.2 and through step 3.3
Bevel is plugged together together, is fully contacted two tangent planes, it is ensured that the alignment of two tangent plane forming layers, and interface position is showed money or valuables one carries unintentionally 1 up and down
~2mm；
Step 3.5：Colligation：The stock processed through step 3.4 and scion are carried out into colligation, it is in neat formation with degradable grafting membrane bag
Individual graft union, it is ensured that stock and scion are not separated when rocking；
Step 3.6：Dip in Lasaxing Oilfield：Heating paraffin is melted, when paraffin temperature is in 70~85 DEG C, scion portion of aliging
Position, then carries out dipping in wax, dips in wax depth for 1~2cm；Stored in 0~2 DEG C, humidity are not less than 95% environment after dipping in wax, just
Complete the vitro grafting from root anvil apple sprout.
The specific sterilization method of step 3.1 is：Potassium permanganate is dissolved in the water, it is 0.1% to be configured to mass concentration
The aqueous solution, grafting knife and scissors are stood be put into solution immediately, soak 10~15min, then are dried or dried after being cleaned with clear water.
The beneficial effects of the invention are as follows：A kind of vitro grafting method from root anvil apple sprout, transfers in vitro by with interior
The method for connecing combines stock scion, promotes advantageously forming for callus, substantially increases plant percent；By transferring
Connect and work in storehouse, substantially increase grafting efficiency and quality, save labour costs and reduce the activity duration；And with degradable
Grafting membrane bandage, it, without artificial releasing, can save labour with weathering.The stock adopted from field and scion can be cold
Preserved in storehouse, substantially increase the flexibility of grafting time；The nursery stock for grafting can for a long time preserve after dipping in wax in freezer, and
And can long-distance transportation, improve the output of nursery stock, promote the Multiplication and extension of high quality seedling.
Specific embodiment
A kind of vitro grafting method from root anvil apple sprout of the invention, specific implementation step is as follows：
Step 1：Preparation before grafting：Including building platform, grafting place is carried out disinfection；
Step 1.1：Workbench prepares：Using scaffold building platform, the length of workbench is 2.3~2.5m, width
It is 0.8~1.2m, is highly 0.6~0.8m, and face laying woodwork plate on the table；One is fixed at woodwork plate plane 42cm
The individual and perpendicular baffle plate of woodwork plate, as the standard of graft stock length；
Step 1.2：Grafting place is carried out disinfection：It is 3%~5% 84 thimerosals to grafting place to use mass concentration
Spray disinfectant is carried out, 24~36h of door and window is closed after sprinkling；
Step 2：Choose stock to be grafted and scion；
Step 2.1：The pretreatment of stock：0.6~1.0mm of roots oxidizing, the stock of 8~12mm of limb diameter are chosen, first
Stock is carried out into cutting back pretreatment at 45~50cm, then enters refrigeration house storage, storage requirement is：Temperature is 0~2 DEG C, humidity
It is not less than 90%；
Step 2.2：Scion is harvested：Scion is harvested, collecting time is 2 months mid or late November to next year early and middle ten dayses,
It is required that being carried out after soil freezes；Scion-seed tree requirement has high yield, stable yields, the proterties of high-quality, and stalwartness of growing, without inspection
Epidemic disease object；It is elected to be the branch of scion, it is desirable to which the substantial healthy and strong, sprout of growth is full, no flower bud, size is matched with graft stock；
Step 2.3：Scion is preserved：The scion that will be harvested through step 2.2, first carries out defoliation treatment, removes all blades, so
Bundling and it is put into polybag, refrigeration house storage afterwards, storage temperature is -2~2 DEG C, humidity is 90~95%.
Step 3：Seedling grafting：The nursery stock that will be chosen through step 2 carries out vitro grafting.
Step 3.1：Grafting knife and scissors are sterilized with liquor potassic permanganate：Potassium permanganate is dissolved in the water, is configured to
Mass concentration is 0.1% aqueous solution, founds grafting knife and scissors be put into solution immediately, soaks 10~15min, then use clear water
Dried after cleaning or dried
Step 3.2：Cut stock：By the plate washer of the adjacent workbench in the root of the stock chosen through step 2, mouth selection will be cut
At the top of stock at the sprout of work edge of table, stock and fixed length are cut；Cut inclined-plane：Carried on the back from the stock sprout of fixed length
Face, knife is entered at 1.4~1.6cm of sprout lower section, cuts out the smooth bevel of 2~4cm of length；Switchback：In stock bevel by upper
At 1/4~1/3 down, along stock inclined-plane toward 1~2cm of incision longitudinal incision, and time partial application 1.4~1.6cm of inclined-plane at,
Switchback length is the half of chamfer length；
Step 3.3：Cut scion：Cut at the 0.8~1.2cm of terminal bud top of the scion that will be chosen through step 2, it is desirable to protect
Card scion has 2~4 full buds, and the length of each full bud is 5~6cm；
Step 3.4：Stock and the docking of scion：The scion processed by the stock processed through step 3.2 and through step 3.3
Bevel is plugged together together, is fully contacted two tangent planes, it is ensured that the alignment of two tangent plane forming layers, and interface position is showed money or valuables one carries unintentionally 1 up and down
~2mm；
Step 3.5：Colligation：The stock processed through step 3.4 and scion are carried out into colligation, it is in neat formation with degradable grafting membrane bag
Individual graft union, it is ensured that stock and scion are not separated when rocking；
Step 3.6：Dip in Lasaxing Oilfield：Heating paraffin is melted, when paraffin temperature is in 70~85 DEG C, scion portion of aliging
Position, then carries out dipping in wax, dips in wax depth for 1~2cm；Stored in 0~2 DEG C, humidity are not less than 95% environment after dipping in wax, just
Complete the vitro grafting from root anvil apple sprout.
In step 1.1 of the present invention, a baffle plate perpendicular with woodwork plate is fixed at the woodwork plate plane 42cm of workbench,
Stock length can quickly and easily be determined, it is ensured that the entirety of stock is consistent, and can improve operating efficiency.
In step 3.5 of the present invention, using degradable bandage, rear bandage is degraded with time weathering in plantation to ground, without people
Work is extractd, and reduces labour, reduces cost, and do not produce white pollution, Environmental Safety.
In step 3.6 of the present invention, dipping in Lasaxing Oilfield can reduce the water loss of grafting, so as to extend the preservation of grafting
Time.
Embodiment 1
Preparation before grafting：Including building platform, grafting place is carried out disinfection；Wherein, workbench prepares：Adopt
With scaffold building platform, the length of workbench is 2.3m, and width is 0.8m, is highly 0.6m, and face paving on the table
If woodwork plate；A baffle plate perpendicular with woodwork plate is fixed at woodwork plate plane 42cm, as the mark of graft stock length
It is accurate；Grafting place is carried out disinfection：Use mass concentration carries out spray disinfectant to grafting place for 3% 84 thimerosals, sprinkling
After close door and window 24h；Choose stock to be grafted and scion；The pretreatment of stock：Choose roots oxidizing 0.6mm, limb diameter 8mm
Stock, stock is first carried out into cutting back pretreatment at 45cm, then enter refrigeration house storage, storage requirement is：Temperature is 0 DEG C, wet
Degree is not less than 90%；Scion is harvested：Scion is harvested, collecting time is 2 months mid or late November to next year early and middle ten dayses, it is desirable to
Carried out after soil freezes；Scion-seed tree requirement has high yield, stable yields, the proterties of high-quality, and stalwartness of growing, right without quarantining
As；It is elected to be the branch of scion, it is desirable to which the substantial healthy and strong, sprout of growth is full, no flower bud, size is matched with graft stock；Scion is protected
Deposit：The scion that will be harvested, first carries out the defoliation treatment all blades of removal, then bundling and is put into polybag, refrigeration house storage, stores
Temperature is deposited for -2 DEG C, humidity is 90%.Seedling grafting：The nursery stock of selection is carried out into vitro grafting.With liquor potassic permanganate to transferring
Connect knife and scissors sterilization：Potassium permanganate is dissolved in the water, the aqueous solution that mass concentration is 0.1% is configured to, immediately by grafting
Knife and scissors stand and are put into solution, soak 10min, then dry or dry after being cleaned with clear water and cut stock：The stock that will be chosen
The adjacent workbench in root plate washer, mouth selection will be cut at the top of the stock at the sprout of work edge of table, cut stock simultaneously
Fixed length；Cut inclined-plane：From the stock sprout back side of fixed length, knife is entered at the 1.4cm of sprout lower section, cut out the smooth of length 2cm
Inclined-plane；Switchback：At stock bevel basipetal 1/4, along stock inclined-plane toward incision 1cm longitudinal incision, and in inclined-plane 1.4cm
Locate back partial application, switchback length is the half of chamfer length；Cut scion：To be cut at the terminal bud top 0.8cm of the scion of selection
It is disconnected, it is desirable to ensure that scion has 2 full buds, the length of each full bud is 5cm；Stock and the docking of scion：The anvil that will be processed
Wood is fully contacted two tangent planes together with being plugged together with the bevel of scion, it is ensured that two tangent plane forming layer alignment, and makes interface position
Show money or valuables one carries unintentionally 1mm up and down；Colligation：The stock for the treatment of and scion are carried out into colligation, with neat formation graft union of degradable grafting membrane bag, it is ensured that
Stock and scion are not separated when rocking；Dip in Lasaxing Oilfield：Heating paraffin is melted, when paraffin temperature is in 70 DEG C, alignment connects
Fringe portion position, then carries out dipping in wax, dips in wax depth for 1cm；Stored in 0 DEG C, the environment of humidity 95% after dipping in wax, just completed certainly
The vitro grafting of root anvil apple sprout.
Embodiment 2
Preparation before grafting：Including building platform, grafting place is carried out disinfection；Wherein, workbench prepares：Adopt
With scaffold building platform, the length of workbench is 2.4m, and width is 1.0m, is highly 0.7m, and face paving on the table
If woodwork plate；A baffle plate perpendicular with woodwork plate is fixed at woodwork plate plane 42cm, as the mark of graft stock length
It is accurate；Grafting place is carried out disinfection：Use mass concentration carries out spray disinfectant to grafting place for 4% 84 thimerosals, sprinkling
After close door and window 30h；Choose stock to be grafted and scion；The pretreatment of stock：Choose roots oxidizing 0.8mm, limb diameter
The stock of 10mm, first carries out cutting back pretreatment by stock at 47cm, then enters refrigeration house storage, and storage requirement is：Temperature is 1
DEG C, humidity 92%；Scion is harvested：Scion is harvested, collecting time is 2 months mid or late November to next year early and middle ten dayses, it is desirable to
Carried out after soil freezes；Scion-seed tree requirement has high yield, stable yields, the proterties of high-quality, and stalwartness of growing, right without quarantining
As；It is elected to be the branch of scion, it is desirable to which the substantial healthy and strong, sprout of growth is full, no flower bud, size is matched with graft stock；Scion is protected
Deposit：The scion that will be harvested, first carries out the defoliation treatment all blades of removal, then bundling and is put into polybag, refrigeration house storage, stores
Temperature is deposited for 0 DEG C, humidity is 92%.Seedling grafting：The nursery stock of selection is carried out into vitro grafting.With liquor potassic permanganate to grafting
Knife and scissors are sterilized：Potassium permanganate is dissolved in the water, the aqueous solution that mass concentration is 0.1% is configured to, immediately by grafting knife
Stood with scissors and be put into solution, soak 12min, then dried or dry after being cleaned with clear water and cut stock：The stock that will be chosen
The plate washer of the adjacent workbench in root, will cut mouth selection and is close at the sprout of work edge of table at the top of stock, cuts stock and determines
It is long；Cut inclined-plane：From the stock sprout back side of fixed length, knife is entered at the 1.5cm of sprout lower section, cut out the smooth oblique of length 3cm
Face；Switchback：At stock bevel basipetal 7/24, along stock inclined-plane toward incision 1.5cm longitudinal incision, and on inclined-plane
Partial application is returned at 1.5cm, switchback length is the half of chamfer length；Cut scion：At the terminal bud top 1cm of the scion that will be chosen
Cut, it is desirable to ensure that scion there are 3 full buds, the length of each full bud is 5.5cm；Stock and the docking of scion：Will treatment
Stock plugged together with the bevel of scion together with, be fully contacted two tangent planes, it is ensured that the alignment of two tangent plane forming layers, and make interface
Position shows money or valuables one carries unintentionally 1.5mm up and down；Colligation：The stock for the treatment of and scion are carried out into colligation, with the in neat formation grafting of degradable grafting membrane bag
Mouthful, it is ensured that stock and scion are not separated when rocking；Dip in Lasaxing Oilfield：Heating paraffin is melted, when paraffin temperature is in 75 DEG C,
Alignment scion position, then carries out dipping in wax, dips in wax depth for 1.5cm；Stored in 1 DEG C, the environment of humidity 97% after dipping in wax, just
Complete the vitro grafting from root anvil apple sprout.
Embodiment 3
Preparation before grafting：Including building platform, grafting place is carried out disinfection；Wherein, workbench prepares：Adopt
With scaffold building platform, the length of workbench is 22.5m, and width is 1.2m, is highly 0.8m, and face paving on the table
If woodwork plate；A baffle plate perpendicular with woodwork plate is fixed at woodwork plate plane 42cm, as the mark of graft stock length
It is accurate；Grafting place is carried out disinfection：Use mass concentration carries out spray disinfectant to grafting place for 5% 84 thimerosals, sprinkling
After close door and window 36h；Choose stock to be grafted and scion；The pretreatment of stock：Choose roots oxidizing 1.0mm, limb diameter
The stock of 12mm, first carries out cutting back pretreatment by stock at 50cm, then enters refrigeration house storage, and storage requirement is：Temperature is 2
DEG C, humidity 94%；Scion is harvested：Scion is harvested, collecting time is 2 months mid or late November to next year early and middle ten dayses, it is desirable to
Carried out after soil freezes；Scion-seed tree requirement has high yield, stable yields, the proterties of high-quality, and stalwartness of growing, right without quarantining
As；It is elected to be the branch of scion, it is desirable to which the substantial healthy and strong, sprout of growth is full, no flower bud, size is matched with graft stock；Scion is protected
Deposit：The scion that will be harvested, first carries out the defoliation treatment all blades of removal, then bundling and is put into polybag, refrigeration house storage, stores
Temperature is deposited for 2 DEG C, humidity is 95%.Seedling grafting：The nursery stock of selection is carried out into vitro grafting.With liquor potassic permanganate to grafting
Knife and scissors are sterilized：Potassium permanganate is dissolved in the water, the aqueous solution that mass concentration is 0.1% is configured to, immediately by grafting knife
Stood with scissors and be put into solution, soak 15min, then dried or dry after being cleaned with clear water and cut stock：The stock that will be chosen
The plate washer of the adjacent workbench in root, will cut mouth selection and is close at the sprout of work edge of table at the top of stock, cuts stock and determines
It is long；Cut inclined-plane：From the stock sprout back side of fixed length, knife is entered at the 1.6cm of sprout lower section, cut out the smooth oblique of length 4cm
Face；Switchback：At stock bevel basipetal 1/3, along stock inclined-plane toward incision 2cm longitudinal incision, and at inclined-plane 1.6cm
Partial application is returned, switchback length is the half of chamfer length；Cut scion：Cut at the terminal bud top 1.2cm of the scion that will be chosen,
It is required that ensureing that scion has 4 full buds, the length of each full bud is 6cm；Stock and the docking of scion：Will process stock and
The bevel of scion is plugged together together, is fully contacted two tangent planes, it is ensured that two tangent plane forming layer alignment, and makes interface position upper and lower
Show money or valuables one carries unintentionally 2mm；Colligation：The stock for the treatment of and scion are carried out into colligation, with neat formation graft union of degradable grafting membrane bag, it is ensured that stock
Do not separated when rocking with scion；Dip in Lasaxing Oilfield：Heating paraffin is melted, when paraffin temperature is in 85 DEG C, scion portion of aliging
Position, then carries out dipping in wax, dips in wax depth for 2cm；Stored in 2 DEG C, the environment of humidity 98% after dipping in wax, just completed from root anvil
The vitro grafting of apple sprout.
Claims (3)
1. a kind of vitro grafting method from root anvil apple sprout, it is characterised in that specifically implement according to following steps：
Step 1：Preparation before grafting：Including building platform, grafting place is carried out disinfection；
Specific implementation step is：
Step 1.1：Workbench prepares：Using scaffold building platform, and face laying woodwork plate on the table；In woodwork plate
A baffle plate perpendicular with woodwork plate is fixed at plane 42cm, as the standard of graft stock length；
Step 1.2：Grafting place is carried out disinfection：Use mass concentration is carried out for 3%~5% 84 thimerosals to grafting place
Spray disinfectant, closes 24~36h of door and window after sprinkling；
Step 2：Choose stock to be grafted and scion；
Specific implementation step is：
Step 2.1：The pretreatment of stock：0.6~1.0mm of roots oxidizing, the stock of 8~12mm of limb diameter are chosen, first by anvil
Wood carries out cutting back pretreatment at 45~50cm, then enters refrigeration house storage, and storage requirement is：Temperature is 0~2 DEG C, and humidity is not low
In 90%；
Step 2.2：Scion is harvested：Scion is harvested, collecting time is 2 months mid or late November to next year early and middle ten dayses, it is desirable to
Carried out after soil freezes；Scion-seed tree requirement has high yield, stable yields, the proterties of high-quality, and stalwartness of growing, right without quarantining
As；It is elected to be the branch of scion, it is desirable to which the substantial healthy and strong, sprout of growth is full, no flower bud, size is matched with graft stock；
Step 2.3：Scion is preserved：The scion that will be harvested through step 2.2, first carries out defoliation treatment, removes all blades, then beats
Tie and be put into polybag, refrigeration house storage, storage temperature is -2~2 DEG C, humidity is 90~95%；
Step 3：Seedling grafting：The nursery stock that will be chosen through step 2 carries out vitro grafting；
Specific implementation step is：
Step 3.1：Grafting knife and scissors are sterilized with liquor potassic permanganate；
Step 3.2：Cut stock：By the plate washer of the adjacent workbench in the root of the stock chosen through step 2, mouth selection will be cut in anvil
Stock and fixed length are cut at the sprout of work edge of table in wooden top；Cut inclined-plane：From the stock sprout back side of fixed length, away from
Enter knife away from 1.4~1.6cm of sprout lower section, cut out the smooth bevel of 2~4cm of length；Switchback：It is basipetal in stock bevel
At 1/4~1/3, along stock inclined-plane toward 1~2cm of incision longitudinal incision, and time partial application 1.4~1.6cm of inclined-plane at, switchback is long
Spend the half for chamfer length；
Step 3.3：Cut scion：Cut at the 0.8~1.2cm of terminal bud top of the scion that will be chosen through step 2, it is desirable to which guarantee connects
Fringe has 2~4 full buds, and the length of each full bud is 5~6cm；
Step 3.4：Stock and the docking of scion：The bevel of the scion processed by the stock processed through step 3.2 and through step 3.3
Plug together together, be fully contacted two tangent planes, it is ensured that the alignment of two tangent plane forming layers, and make interface position show money or valuables one carries unintentionally 1 up and down~
2mm；
Step 3.5：Colligation：The stock processed through step 3.4 and scion are carried out into colligation, is transferred in neat formation with degradable grafting membrane bag
Interface, it is ensured that stock and scion are not separated when rocking；
Step 3.6：Dip in Lasaxing Oilfield：Heating paraffin is melted, when paraffin temperature is in 70~85 DEG C, scion position of aliging, so
After carry out dipping in wax, dip in wax depth for 1~2cm；Stored in 0~2 DEG C, humidity are not less than 95% environment after dipping in wax, just completed
From the vitro grafting of root anvil apple sprout.
2. a kind of vitro grafting method from root anvil apple sprout according to claim 1, it is characterised in that the step
3.1 specific sterilization method is：Potassium permanganate is dissolved in the water, the aqueous solution that mass concentration is 0.1% is configured to, immediately will
Grafting knife and scissors are uprightly put into solution, soak 10~15min, then dry or dry after being cleaned with clear water.
3. a kind of vitro grafting method from root anvil apple sprout according to claim 1, it is characterised in that the step
In 1, the length of workbench is 2.3~2.5m, and width is 0.8~1.2m, is highly 0.6~0.8m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510205556.9A CN104770225B (en) | 2015-04-27 | 2015-04-27 | A kind of vitro grafting method from root anvil apple sprout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510205556.9A CN104770225B (en) | 2015-04-27 | 2015-04-27 | A kind of vitro grafting method from root anvil apple sprout |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104770225A CN104770225A (en) | 2015-07-15 |
| CN104770225B true CN104770225B (en) | 2017-06-06 |
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| CN104126396B (en) * | 2014-07-28 | 2016-04-13 | 西北农林科技大学 | A kind ofly breed the method for apple Qiaoization clone roundleaf Malus spectabilis from root anvil seedling |
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