CN104429644A - In-vitro grafting method - Google Patents
In-vitro grafting method Download PDFInfo
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- CN104429644A CN104429644A CN201410834291.4A CN201410834291A CN104429644A CN 104429644 A CN104429644 A CN 104429644A CN 201410834291 A CN201410834291 A CN 201410834291A CN 104429644 A CN104429644 A CN 104429644A
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- grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- Life Sciences & Earth Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
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- Cultivation Of Plants (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an in-vitro grafting method. According to the in-vitro grafting method, a scion and a stock are both in-vitro materials. After grafting, the stock which the scion is grafted onto is rapidly transplanted to a seedling bed, and the purpose that rooting of the stock and union of a connector are synchronous is achieved. By the adoption of the in-vitro grafting technology, grafting is easy to operate, centralization and industrialization of grafting are achieved, and multiple varieties with a low survival rate during traditional grafting or distant varieties can have a high survival rate after the environment optimization technology is adopted; especially, a good promoting function is achieved on inducing of callus tissue of a cut and formation of a fusion vascular bundle between heterogeneous cells, supplementation of a nutrient solution and exogenous hormones can be achieved in the integrally optimized seedling bed, and the union survival rate is greatly increased; the in-vitro grafting method has also the positive function on rapid cultivation of various induced seedlings during breeding.
Description
Technical field
The invention belongs to agricultural technology field, particularly relate to a kind of in vitro grafting method.
Background technology
Tradition grafting is carry out grafting on the stock of ground cultivation mostly, and grafting twig must be waited after grafting to survive rear portable.When cultivation time of stock adds the cultivation of grafting, the whole growing-seedling period of chien shih is long, and some kind takes 2-3, and what the cycle was short also takes 1 year, greatly have impact on speed and the efficiency of nursery.And stock tree can only grafting once, grafting needs to cultivate a large amount of stock trees.In addition the survival rate of traditional grafting is very big by the extraneous impact from boundary's environment, and the change as the climatic factor such as humidity, temperature, illumination of instability all can affect survival rate, particularly meets rainy weather or high temperature and drought weather will make survival rate reduce greatly.
Summary of the invention
In view of this, the invention provides a kind of in vitro grafting method, improve the output of grafting, be convenient to centralized management, save the time.
For solving above technical problem, technical scheme of the present invention, for adopting a kind of in vitro grafting method, comprises the grafting after being with pile to extract soil, comprises the following steps:
1) stock variety and scion variety adaptive is with it selected;
2) adopt brachyplast to connect grafting grafting on a stock some scion, then the stock of scion in grafting is cut branch section and to carry out disinfection the process of leaching root;
3) on the bedding injecting in advance nutrient solution, cuttage has the branch section of grafting, and with bamboo chip support arch, glass film covers, then grafting is taken root and interface healing, survive after process.
Preferably, described step 2 grafting order be from ending successively and, and grafting one section of cutting one section is so that binding.
Preferably, described step 2 can also adopt the grafting method of bud grafting.
Preferably, the nutrient solution on described step 3 bedding has 7-9cm.
Preferably, described step 3 grafting takes root and the management of interface healing can also arrange constant temperature, constant humidity, light irradiation apparatus on seedbed, promotes the healing of interface and the rooting rate of grafting.
Preferably, the described grafting phase of taking root keeps temperature 22 DEG C-30 DEG C, and air humidity remains on 50-70 degree; Interface healing stage keeps temperature 28 DEG C-30 DEG C, and air humidity is no more than in 65 degree, and ventilation.
Preferably, described step 3 is not eager the grafting taken root, and can bundledly endwaysly be deposited on smooth bedding face, bamboo chip support arch, glass film covers.
Preferably, the grafting of not being eager the to take root phase of taking root keeps temperature 22 DEG C-30 DEG C, and air humidity remains on 50-75 degree; Interface healing stage keeps temperature to be no more than 15 DEG C, and air humidity is no more than in 45 degree, and ventilation, be inserted into land for growing field crops after surviving or cultivate bed.
The present invention compared with prior art, by some scion graftings on a stock, graft rear intercept, rooting treatment, move on to seedbed, grafting operation is made easily to realize centralization, and by the kind of the low kind of many traditional graft survival rates or edge far away can be made after environment optimization technology to reach higher survival rate.The fascicular formation of fusion particularly between the induction and foreign cell of incision wound tissue has good facilitation, and in the seedbed of this centralized optimization, also can realize supplementing of nutrient solution and exogenous hormone, substantially increase and heal into motility rate.
Embodiment
In order to make those skilled in the art understand technical scheme of the present invention better, below in conjunction with embodiment, the present invention is described in further detail.
Embodiment one
A kind of in vitro grafting method, comprises the following steps:
1) stock variety and scion variety adaptive is with it selected: pumpkin seedling can be selected to be stock, and muskmelon seedling is scion, and pumpkin seedling plant height is close with muskmelon seedling plant height.
2) 3-5 scion is adopted be abutted against grafting grafting on a stock, then the stock of scion in grafting is cut branch section and to carry out disinfection the process of leaching root;
Remove pumpkin seedling true leaf, by pumpkin seedling and muskmelon seedling close, then two branches selecting branch thickness to be more or less the same, by pumpkin seedling and the segmentation of muskmelon seedling. prop up leaf parallel direction with blade along pumpkin, the plane of 3 ~ 5 centimeter length of splaying in 20-30 degree, expose and form layer and xylem, same muskmelon seedling tangent plane can connect with pumpkin seedling tangent plane, formation layer is aligned with each other, uses plastics tighten, muskmelon seedling stays two or three to cut off; Then the pumpkin seedling branch section intercept of muskmelon seedling in grafting, then carry out disinfection the process of leaching root.
3) being equipped with in the bedding of 7-9cm nutrient solution by the muskmelon seedling cuttage of grafting, and with bamboo chip support arch, glass film cover, carry out grafting take root and interface healing, survive after process:
Bedding also arranges constant temperature, constant humidity, light irradiation apparatus, and for pumpkin seedling rooting and interface healing provide superior environment, the phase of taking root keeps temperature 22 DEG C-30 DEG C, and air humidity remains on 50-70 degree; Interface healing stage keeps temperature 28 DEG C-30 DEG C, and air humidity is no more than in 65 degree, and ventilation and penetrating light is until grafting survives.
Wherein, the grafting taken root of not being eager, can bundledly endwaysly be deposited on smooth bedding face, bamboo chip support arch, and glass film covers, and its phase of taking root keeps temperature 22 DEG C-30 DEG C, and air humidity remains on 50-75 degree; Interface healing stage keeps temperature to be no more than 15 DEG C, and air humidity is no more than in 45 degree, and ventilation, be inserted into land for growing field crops after surviving or cultivate bed.
Adopt original method and above grafting method to carry out grafting respectively, and choose homoplastic graft raw material, different stock raw materials, under same environmental conditions, the muskmelon seedling that above four kinds of mode graftings go out is carried out survival rate test, and test result is in table 1.
Table 1-two kind of grafting method grafting muskmelon shoot survival percent test result
Pass through above table, the muskmelon seedling of the application's grafting can be learnt, no matter be adopt pumpkin seedling to do stock, or adopt mahogany to do stock, the survival rate of its muskmelon seedling, all more than 90%, makes the kind of the low kind of many traditional graft survival rates or edge far away reach higher survival rate compared to traditional grafting method.And in the seedbed of this centralized optimization, also can realize supplementing of nutrient solution and exogenous hormone, substantially increase and heal into motility rate, and then improve the output of grafting.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred embodiment should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (8)
1. an in vitro grafting method, comprises the grafting after being with pile to extract soil, it is characterized in that, comprise the following steps:
1) stock variety and scion variety adaptive is with it selected;
2) adopt brachyplast to connect grafting grafting on a stock some scion, then the stock of scion in grafting is cut branch section and to carry out disinfection the process of leaching root;
3) on the bedding injecting in advance nutrient solution, cuttage has the branch section of grafting, and with bamboo chip support arch, glass film covers, then grafting is taken root and interface healing, survive after process.
2. the in vitro grafting method of one according to claim 1, is characterized in that, described step 2 grafting order be from ending successively and, and grafting one section of cutting one section is so that binding.
3. the in vitro grafting method of one according to claim 1, is characterized in that, the grafting method that described step 2 can also adopt bud grafting, be abutted against.
4. the in vitro grafting method of one according to claim 1, is characterized in that, the nutrient solution on described step 3 bedding has 7-9cm.
5. the in vitro grafting method of one according to claim 1, is characterized in that, described step 3 grafting takes root and the management of interface healing can also arrange constant temperature, constant humidity, light irradiation apparatus on seedbed, promotes the healing of interface and the rooting rate of grafting.
6. the in vitro grafting method of one according to claim 5, is characterized in that, the described grafting phase of taking root keeps temperature 22 DEG C-30 DEG C, and air humidity remains on 50-70 degree; Interface healing stage keeps temperature 28 DEG C-30 DEG C, and air humidity is no more than in 65 degree, and ventilation.
7. the in vitro grafting method of one according to claim 1, is characterized in that, described step 3 is not eager the grafting taken root, and can bundledly endwaysly be deposited on smooth bedding face, bamboo chip support arch, glass film covers.
8. the in vitro grafting method of one according to claim 7, is characterized in that, the grafting of not being eager the to take root phase of taking root keeps temperature 22 DEG C-30 DEG C, and air humidity remains on 50-75 degree; Interface healing stage keeps temperature to be no more than 15 DEG C, and air humidity is no more than in 45 degree, and ventilation, be inserted into land for growing field crops after surviving or cultivate bed.
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| CN201410834291.4A CN104429644A (en) | 2014-12-26 | 2014-12-26 | In-vitro grafting method |
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| CN201410834291.4A CN104429644A (en) | 2014-12-26 | 2014-12-26 | In-vitro grafting method |
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| CN104429644A true CN104429644A (en) | 2015-03-25 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104770225A (en) * | 2015-04-27 | 2015-07-15 | 陕西超越农业有限公司 | In-vitro grafting method for self-rooted rootstock apple bud seedling |
| CN108156989A (en) * | 2018-02-06 | 2018-06-15 | 江苏沿江地区农业科学研究所 | Forest vitro grafting method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144499A (en) * | 2011-02-22 | 2011-08-10 | 广西大学 | Method for overwintering cultivation and perennial cuttage propagation of red ramie |
| CN102907264A (en) * | 2012-10-13 | 2013-02-06 | 南京市蔬菜科学研究所 | Method for raising watermelon seedlings by dual-root-cutting grafting |
| CN103210793A (en) * | 2013-01-28 | 2013-07-24 | 浙江省农业科学院 | Single-stock and multi-scion grafting seedling culturing method of oil camellia |
| CN103229672A (en) * | 2013-04-23 | 2013-08-07 | 安徽省农业科学院园艺研究所 | Mini watermelon grafting and seedlings raising method |
| CN104126396A (en) * | 2014-07-28 | 2014-11-05 | 西北农林科技大学 | Method for propagating vigorous clonal Malus prunifolia own-rooted stock seedlings of apple trees |
-
2014
- 2014-12-26 CN CN201410834291.4A patent/CN104429644A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144499A (en) * | 2011-02-22 | 2011-08-10 | 广西大学 | Method for overwintering cultivation and perennial cuttage propagation of red ramie |
| CN102907264A (en) * | 2012-10-13 | 2013-02-06 | 南京市蔬菜科学研究所 | Method for raising watermelon seedlings by dual-root-cutting grafting |
| CN103210793A (en) * | 2013-01-28 | 2013-07-24 | 浙江省农业科学院 | Single-stock and multi-scion grafting seedling culturing method of oil camellia |
| CN103229672A (en) * | 2013-04-23 | 2013-08-07 | 安徽省农业科学院园艺研究所 | Mini watermelon grafting and seedlings raising method |
| CN104126396A (en) * | 2014-07-28 | 2014-11-05 | 西北农林科技大学 | Method for propagating vigorous clonal Malus prunifolia own-rooted stock seedlings of apple trees |
Non-Patent Citations (6)
| Title |
|---|
| 吴以专: ""浅析茶花扦插育苗关键技术"", 《福建农业》 * |
| 康健滋: "快速成型法——先嫁接后扦插"", 《中国花卉盆景》 * |
| 曾现春: ""月季早春插接育苗技术"", 《中国花卉盆景》 * |
| 洛阳等: ""牡丹快速繁殖新法——一砧(芍药根)多穗(牡丹芽)嫁接法"", 《中国花卉盆景》 * |
| 王彦杰等: ""菊花扦插繁育与管理"", 《中国花卉园艺》 * |
| 王朋成等: ""瓜类改良插接法嫁接技术"", 《中国瓜菜》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104770225A (en) * | 2015-04-27 | 2015-07-15 | 陕西超越农业有限公司 | In-vitro grafting method for self-rooted rootstock apple bud seedling |
| CN108156989A (en) * | 2018-02-06 | 2018-06-15 | 江苏沿江地区农业科学研究所 | Forest vitro grafting method |
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Application publication date: 20150325 |