CN104684934A - 一种抗草甘膦融合蛋白及其编码基因、产生方法与应用 - Google Patents

一种抗草甘膦融合蛋白及其编码基因、产生方法与应用 Download PDF

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CN104684934A
CN104684934A CN201380008110.6A CN201380008110A CN104684934A CN 104684934 A CN104684934 A CN 104684934A CN 201380008110 A CN201380008110 A CN 201380008110A CN 104684934 A CN104684934 A CN 104684934A
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plant
glyphosate
epsps
cotton
seq
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CN104684934B (zh
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崔洪志
何云蔚
王建胜
孙超
王君丹
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Genesis Seed Industry Co ltd
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Genesis Seed Industry Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
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  • Biotechnology (AREA)
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  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

提供了一种5-烯醇丙酮莽草酸-3-磷酸合成酶(EPSPS)融合蛋白，其编码基因，和获得该融合蛋白的方法。还提供了该5-烯醇丙酮莽草酸-3-磷酸合成酶融合蛋白在培育抗草甘膦植物中的应用。

Description

PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201380008110.6A 2013-08-26 2013-08-26 一种抗草甘膦融合蛋白及其编码基因、产生方法与应用 Expired - Fee Related CN104684934B (zh)

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PCT/CN2013/082238 WO2015027369A1 (zh) 2013-08-26 2013-08-26 一种抗草甘膦融合蛋白及其编码基因、产生方法与应用

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CN104684934A true CN104684934A (zh) 2015-06-03
CN104684934B CN104684934B (zh) 2017-07-07

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106318967B (zh) * 2015-06-15 2020-08-14 创世纪种业有限公司 一种含有抗草甘膦基因的植物表达载体及其应用
CN106350532A (zh) * 2016-08-28 2017-01-25 浙江大学 一种抗草甘膦融合基因、编码蛋白及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101619319A (zh) * 2009-03-13 2010-01-06 创世纪转基因技术有限公司 人工改造合成的抗草甘膦基因与应用
CN102399794A (zh) * 2010-09-08 2012-04-04 创世纪转基因技术有限公司 一种棉花epsp合成酶突变体基因及其应用
CN102643786A (zh) * 2012-04-12 2012-08-22 北京奥瑞金种业股份有限公司 抗耐草甘膦蛋白G2-aroA的单克隆抗体及其应用
CN102676553A (zh) * 2012-04-12 2012-09-19 北京奥瑞金种业股份有限公司 一种人工合成的耐草甘膦基因及其应用
WO2012148275A1 (en) * 2011-04-29 2012-11-01 Keygene N.V. Glyphosate resistance enhancement

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103409445A (zh) * 2013-08-01 2013-11-27 刘坚 抗草甘膦基因mtp-smg2-epsps及其在培育抗草甘膦玉米中的应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101619319A (zh) * 2009-03-13 2010-01-06 创世纪转基因技术有限公司 人工改造合成的抗草甘膦基因与应用
CN102399794A (zh) * 2010-09-08 2012-04-04 创世纪转基因技术有限公司 一种棉花epsp合成酶突变体基因及其应用
WO2012148275A1 (en) * 2011-04-29 2012-11-01 Keygene N.V. Glyphosate resistance enhancement
CN102643786A (zh) * 2012-04-12 2012-08-22 北京奥瑞金种业股份有限公司 抗耐草甘膦蛋白G2-aroA的单克隆抗体及其应用
CN102676553A (zh) * 2012-04-12 2012-09-19 北京奥瑞金种业股份有限公司 一种人工合成的耐草甘膦基因及其应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TONG X.H.: "Accession ID:ABV24481, 5-enolpyruvylshikimate-3-phoshpate synthase[Gossypium hirsutum]", 《NCBI GENBANK》 *
TONG X.H.: "Accession ID:EU123855, Gossypium hirsutum 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS) mRNA,complete cds", 《NCBI GENBANK》 *

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CN104684934B (zh) 2017-07-07

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Address after: 518117 7th floor, Chuangshi seed industry building, A701, No.22, Puzi Road, Pingdi street, Longgang District, Shenzhen, Guangdong Province

Patentee after: Biocentury Seed Industry Co., Ltd.

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Granted publication date: 20170707

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