CN104620837B - The booth set small arched shed cuttage and seedling culture method of high and cold two nether region tetraploid Radix Scutellariaes - Google Patents
The booth set small arched shed cuttage and seedling culture method of high and cold two nether region tetraploid Radix Scutellariaes Download PDFInfo
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- CN104620837B CN104620837B CN201510099642.6A CN201510099642A CN104620837B CN 104620837 B CN104620837 B CN 104620837B CN 201510099642 A CN201510099642 A CN 201510099642A CN 104620837 B CN104620837 B CN 104620837B
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- cuttage
- arched shed
- small arched
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- booth
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The present invention relates to a kind of booth at high and cold two nether region tetraploid Radix Scutellariaes and overlap small arched shed cuttage and seedling culture method, belong to plant growing technical field.District of the present invention tetraploid Radix Scutellariae cuttage raise seedling technique is improved, and defines a set of high and cold two nether region booth inner sleeve small arched shed cutting propagation fast breeding techniques.Using the method that the present invention provides, cuttage survival rate averagely reaches 96.4%, uses booth set small arched shed cutting propagation, and survival rate is high, simple to operate, saving of work and time, and low cost has feasibility aborning.
Description
Technical field
The present invention relates to a kind of booth at high and cold two nether region tetraploid Radix Scutellariaes and overlap small arched shed cuttage and seedling culture method, belong to
Plant growing technical field.
Background technology
Tetraploid Radix Scutellariae demonstrates clear superiority than common diploid Radix Scutellariae at the aspect such as yield, active constituent content, tool
There is promotion prospect, but tetraploid Radix Scutellariae is bloomed less and evening, observes through for many years, has no solid plant, therefore can not carry out numerous with seed
Grow, asexual propagation can only be carried out with branch cutting.
Summary of the invention
In order to make tetraploid Radix Scutellariae be promoted, make this scientific achievement be applied to produce, ensure clinical drug safety, this
Tetraploid Radix Scutellariae cuttage raise seedling technique is improved by application, it is proposed that in high and cold two nether region booth inner sleeve small arched shed cuttages
Nursery fast breeding technique.
Technical scheme:
The booth set small arched shed cuttage and seedling culture method of high and cold two nether region tetraploid Radix Scutellariaes, comprises the steps:
1st step, planting site is built booth, water, dig, apply fertilizer and pesticide, then ground offered in canopy water channel,
Water channel limit is made little furrow；
2nd step, in the first tenday period of a month in late April to May, from maternal plant, take off branch section, the little furrow in the 1st step carry out cuttage,
After sterilization, little furrow are built small arched shed, and by real by soil pressure around；
After 3rd step, cuttage 1～7 day, small arched shed hid with 90% sunshade net, hid with 50% sunshade net after 7 days, then general
Small arched shed takes off film, continues to cultivate to seedling.
In the 1st described step, between plantation region height above sea level 1900～2400m, pH value is 7.0～9.0, and the soil texture is earth
Soil.
In the 1st described step, water channel width 40cm, deep 30cm, two each 20 cm of canal hem width of water channel.
In the 1st described step, little furrow width 0.8m, long 2.6m, furrow spacing 30cm.
In the 1st described step, fertilising and pesticide apply: mu executes well-rotted farmyard manure 2500kg, phosphorus diammonium 40kg, urine
Element 20kg, phoxim or poison zinc cream 0.5kg, carbendazim or thiophanate methyl 0.5kg.
In the 2nd described step, from maternal plant, take off branch section refer to cut 5～7cm length from the eustipes part of maternal plant edible tender branch
Segment.
In the 2nd described step, the spacing in the rows 2～3cm of cuttage, line-spacing 6～7cm, the degree of depth 2～3cm.
In the 3rd described step, in booth, intensity of illumination controls at about 80000lx；After cuttage 1～7 day, light in small arched shed
Illumination is 7000～8000lx, and after cuttage after 7 days, in small arched shed, illuminance is 15000～30000lx.
In the 3rd described step, if late April takes off film in 25 days to late May cutting seedling, small arched shed；If 6～7
The first tenday period of a month moon, cutting seedling, then took off film in 20 days；If cutting seedling in August, then within 30 days, take off film；If JIUYUE cutting seedling, then 40 days
Take off film later.
In the 3rd described step, after cuttage, canopy temperature controls below 35 DEG C daytime, and in small arched shed, temperature controls 38
Below DEG C, 5～10 cm ground temperature are below 30 DEG C；Night, canopy temperature was more than 10 DEG C, and in small arched shed, temperature controls at 13 DEG C
Above.
In the 3rd described step, after cuttage, booth humidity controls more than 65%, small arched shed humidity more than 80%, soil
Moisture is more than 30%.
Beneficial effect
Using the method that the present invention provides, cuttage survival rate averagely reaches 96.4%, and open country cuttage survival rate is up to
46.7%, heliogreenhouse common management cuttage survival rate is up to 51.2%, uses small arched shed cutting propagation in booth, survival rate
Height, simple to operate, saving of work and time, low cost, there is feasibility aborning.
Detailed description of the invention
Below by detailed description of the invention, the present invention is described in further detail.But those skilled in the art will manage
Solving, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.Unreceipted concrete skill in embodiment
Art or condition person, according to the technology described by the document in this area or condition or carried out according to product description.Examination used
Agent or instrument unreceipted production firm person, be can by city available from conventional products.
Heretofore described well-rotted farmyard manure is to select one or several the mixing in horse, cattle, sheep or pig manure
Through becoming thoroughly decomposed 30～40 days gained.
The stem high 25～35cm of the tetraploid Radix Scutellariae maternal plant in following example；Life, blade are draped over one's shoulders needle-like by leaf；Network of roots is raw, and 2
～6, root length 15 about cm.
Embodiment 1
1 selection of land builds canopy
Selecting between height above sea level 1900～2400m, pH value is 7.0～9.0, and the soil texture is loam, without foreign material ground such as stones
Block, at late March to early and middle ten days in April, builds or builds steelframe booth, meanwhile, on original tetraploid Radix Scutellariae maternal plant ground with bamboo chip
Block scaffolding epiphragma, applies fertilizer, watering makes it do sth. in advance germination and emergence fast-growth, and the row culture cuttage that sends forth branches is used.
2 whole ground beddings
After booth is built up, water permeable once, after 3～4 days land for building are dry, soil deeptillage, in conjunction with ploughing, mu in booth executes corruption
Ripe farm manure 2500kg, phosphorus diammonium 40kg, carbamide 20kg, phoxim or poison zinc cream 0.5kg, carbendazim or thiophanate methyl
0.5kg, picks up the mulch film that gives up to the greatest extent, stone, tile, preceding crop Fructus Citri tangerinae stalk, root stubble etc., and a kind of farm tools of raking is thin, then along booth length in the middle of ground
Open a wide 40cm, deep 30cm water channel, two each 20 cm of canal hem width, canopy is divided into two pieces along canopy length.Soil, both sides press 0.8m width,
Long 2.6m is made little furrow, furrow spacing 30cm.
3 beta pruning cuttages, take small arched shed
The first tenday period of a month in late April to May, when maternal plant branch grows to 10～15cm, before being selected at 10 in fine day morning, or afternoon 5
After Dian, or select overcast and rainy, cut the segment of 5～7cm length from the eustipes part of maternal plant edible tender branch, by spacing in the rows 2～3cm,
Line-spacing 6～7cm, in the degree of depth 2～3cm cuttage to little furrow, has often inserted furrow, adds 75% Bravo 25g disinfectant with 100kg water and waters
Permeable, immediately with No. 8 iron wires, it is cut into the long segment of 1m, every furrow 4～5, curves semicircle and make frame, make with 1.2m covering with ground sheeting
Small arched shed, real by soil pressure around small arched shed, prevent gas leakage, be then that 90% sunshade net hides with shading rate.
Manage after 4 cuttages
4.1 temperature treatment
After cuttage, canopy temperature controls below 35 DEG C daytime, should take regulating tuyere in time, pours water etc. and to arrange when exceeding
Executing, reduce temperature, in small arched shed, temperature controls below 38 DEG C, and 5～10 cm ground temperature, below 30 DEG C, should water when exceeding in time
Little water for cooling.Night, canopy temperature was more than 10 DEG C, and in small arched shed, temperature controls more than 13 DEG C.
4.2 moisture management
After cuttage, booth humidity controls more than 65%, and small arched shed humidity more than 80%, soil moisture is more than 30%.
4.3 intensity of illumination management
After cuttage 1～7 day, in booth, intensity of illumination hid with 90% sunshade net at about 80000lx, small arched shed, small arched shed
Interior intensity of illumination after 7000～8000lx, 7 days with 50% sunshade net covering, in small arched shed intensity of illumination 15000～
30000lx。
4.4 small arched shed film-uncovering time
Late April, small arched shed took off film for 25 days to late May cutting seedling, entered normal management, and normal management includes pulling out at any time
Cut weeds, soil moisture see dry see wet, prevention and elimination of disease and pests at any time.
(it addition, if 6～early July cutting seedling, then within 20 days, take off film, enter normal management；If cuttage in August
Seedling, then take off film for 30 days, enters normal management；If JIUYUE cutting seedling, then take off film after 40 days, enter normal management.)
4.5 pest management
Easily there is powdery mildew after entering late July in tetraploid Radix Scutellariae cutting seedling, by 25% triadimefon wettability after generation
800 times of liquid of powder, 50% carbendazol wettable powder 500～the spraying of 800 times of liquid, 75% Bravo wettable powder 500～600 times
Liquid spraying preventing and treating, after August, aphid easily endangers, with ant worm woods, 2500 times of liquid spraying preventing and treatings of Acetamiprid.
Reference examples 1
Difference with embodiment 1 is: uses greenhouse gardening, does not uses small arched shed.Other implantation time, management method are all
Identical.
Reference examples 2
Difference with embodiment 1 is: in the management of step 4.3 illuminance, and small arched shed is provided without sunshade net and hides, not to little
The intensity of illumination of bow canopy is controlled, and other way to manage, film-uncovering time etc. are the most identical.
That is: after cuttage 1～7 day, in booth, illuminance was in about 80000lx, small arched shed covering with ground sheeting, late April to 5
Month last ten-days period cutting seedling, small arched shed is taken off film for 25 days, is entered normal management.
Reference examples 3
Difference with embodiment 1 is: the time choosing new branch in the 3rd step from maternal plant is on late April to May
In ten days, between 12 o'clock to 2 o'clock of fine day, remaining plantation is the most identical with management method.
Reference examples 4
Difference with embodiment 1 is: do not use the seedling raising manners of booth, small arched shed, complete outdoor plantation.
Reference examples 5
Difference with embodiment 1 is: in the 3rd step, and the position intercepting segment from maternal plant is position between stem, and non-solid
Execute the stipes position in example 1.Other cuttage, management method are the most identical.
In above example and reference examples, the tetraploid Radix Scutellariae of plantation is all in late October seedling then, investigates whole educating
Survival rate during Seedling, single plant yield, content of baicalin.
Wherein, the detection method of content of baicalin uses HPLC chromatogram method to measure.
Chromatographic condition: Agilent 1100 high performance liquid chromatograph；Chromatographic column: C18；Flowing phase: methanol-water-phosphoric acid (47:
53:0.2)；Detection wavelength: 280 nm；Flow velocity: 1.0 ml/min；Column temperature: 25 DEG C.
Prepared by reference substance solution: the baicalin that vacuum desiccator is dried is made into the 1ml solution containing 60 μ g and get final product.
Prepared by need testing solution: take tetraploid Baical Skullcap Root (cross 80 mesh sieves) about 0. 3g, accurately weighed adds 70% ethanol
40ml is heated to reflux 3h, lets cool, and filters, and filtrate puts l00ml volumetric flask, with a small amount of 70% ethanol washing container and residue by several times, washes
In the liquid filter same volumetric flask of people, adding 70% ethanol and be settled to scale, the accurate 0.5ml. of absorption adds in 5ml volumetric flask, adds methanol fixed
Hold to scale, shake up and get final product.
Accurate test sample, each 10 μ 1 of reference substance solution of drawing, injection high performance liquid chromatograph, record peak area, by external standard
Method calculates, and obtains the percentage composition of baicalin in dry product.
Table 1
Embodiment 1 contrasts with reference examples 1, by adding the seedling raising manners of small arched shed in booth, can improve survival rate.
Embodiment 1 and reference examples 2 contrast it can be seen that small arched shed uses sunshade net to hide, and can be effectively improved survival rate and individual plant
Yield.Embodiment 1 contrasts with reference examples 1, it can be seen that by choosing the time of the new branch suitably plucking maternal plant, permissible
Improve survival rate.Embodiment 1 is compared with reference examples 5, and survival rate is high, and this is owing to being positioned at stem by the position of control intercepting sprig
At joint, can preferably improve the growth of branch, intercepting sprig survival rate when stipes position is 96.4%, and between stem position
Time then Radix Scutellariae cuttage survival rate 72.3%, and stem section cutting part is easily formed hollow, causes disease to contaminate.
Claims (1)
- The booth set small arched shed cuttage and seedling culture method of the most high and cold two nether region tetraploid Radix Scutellariaes, it is characterised in that include walking as follows Rapid:1st step, planting site is built booth, water, dig, apply fertilizer and pesticide, then ground offered in canopy water channel, at water Little furrow it are made on canal limit；2nd step, in the first tenday period of a month in late April to May, from maternal plant, take off branch section, the little furrow in the 1st step carry out cuttage, sterilization After, little furrow are built small arched shed, and by real by soil pressure around；After 3rd step, cuttage 1～7 day, small arched shed hid with 90% sunshade net, with 50% sunshade net covering after 7 days, then removed little bow Film taken off by canopy, continues to cultivate to seedling；In the 1st described step, between plantation region height above sea level 1900～2400m, pH value is 7.0～9.0, and the soil texture is loam；In the 1st described step, water channel width 40cm, deep 30cm, two each 20 cm of canal hem width of water channel；In the 1st described step, little furrow width 0.8m, long 2.6m, furrow spacing 30cm；In the 1st described step, fertilising and pesticide apply: mu executes well-rotted farmyard manure 2500kg, phosphorus diammonium 40kg, carbamide 20kg, phoxim or poison zinc cream 0.5kg, carbendazim or thiophanate methyl 0.5kg；In the 2nd described step, from maternal plant, take off branch section refer to cut the little of 5～7cm length from the eustipes part of maternal plant edible tender branch Section；In the 2nd described step, the spacing in the rows 2～3cm of cuttage, line-spacing 6～7cm, the degree of depth 2～3cm；In the 3rd described step, in booth, intensity of illumination controls at about 80000lx；After cuttage 1～7 day, in small arched shed, illumination was strong Degree is 7000～8000lx, and after cuttage after 7 days, in small arched shed, intensity of illumination is 15000～30000lx；In the 3rd described step, if late April takes off film in 25 days to late May cutting seedling, small arched shed；If 6～July on Ten days cutting seedling, then within 20 days, take off film；If cutting seedling in August, then within 30 days, take off film；If JIUYUE cutting seedling, then after 40 days Take off film；In the 3rd described step, after cuttage, canopy temperature controls below 35 DEG C daytime, in small arched shed temperature control 38 DEG C with Under, 5～10 cm ground temperature are below 30 DEG C；Night, canopy temperature was more than 10 DEG C, in small arched shed temperature control 13 DEG C with On；In the 3rd described step, after cuttage, booth humidity controls more than 65%, and small arched shed humidity more than 80%, soil moisture exists More than 30%.
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| CN201510099642.6A CN104620837B (en) | 2015-03-07 | 2015-03-07 | The booth set small arched shed cuttage and seedling culture method of high and cold two nether region tetraploid Radix Scutellariaes |
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| CN105993716A (en) * | 2016-05-28 | 2016-10-12 | 林晶 | Use method for volume-changeable awning-in-greenhouse of plastic-cover greenhouse |
| CN108207516B (en) * | 2018-01-23 | 2020-10-16 | 青海省农林科学院 | Method for cultivating early-maturing cabbage seedlings in high and cold areas by utilizing sunlight greenhouse |
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| CN101569265A (en) * | 2009-06-08 | 2009-11-04 | 昆明理工大学 | Non-tube rapid propagation method of osmanthus |
| CN102084771A (en) * | 2010-11-02 | 2011-06-08 | 冯全君 | Method for culturing baikal skullcap root drug |
| CN103650803A (en) * | 2012-08-28 | 2014-03-26 | 成元 | Planting method of radix scutellariae |
| CN103168584A (en) * | 2012-12-13 | 2013-06-26 | 青岛农业大学 | Short spike cuttage breeding mode for sweet osmanthus in autumn and winter in north |
| CN104303818A (en) * | 2014-11-22 | 2015-01-28 | 毛艳玲 | Cutting propagation technology of radix scutellariae |
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