CN104620837A - Cutting seedling method for tetraploid scutellaria in high and cold double-clouded regions by using greenhouse sleeved with small arch-shaped sheds - Google Patents
Cutting seedling method for tetraploid scutellaria in high and cold double-clouded regions by using greenhouse sleeved with small arch-shaped sheds Download PDFInfo
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- CN104620837A CN104620837A CN201510099642.6A CN201510099642A CN104620837A CN 104620837 A CN104620837 A CN 104620837A CN 201510099642 A CN201510099642 A CN 201510099642A CN 104620837 A CN104620837 A CN 104620837A
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- cuttage
- arched shed
- small arched
- tetraploid
- booth
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention relates to a cutting seedling method for tetraploid scutellaria in high and cold double-clouded regions by using a greenhouse sleeved with small arch-shaped sheds and belongs to the technical field of plant planting. According to the invention, the tetraploid scutellaria cutting seedling technology is improved to form a set of cutting seedling rapid propagation technology for tetraploid scutellaria in high and cold double-clouded regions by using the greenhouse sleeved with small arch-shaped sheds. According to the method provided by the invention, the cutting survival rate averagely reaches 96.4%. By adopting cutting seedling by using the greenhouse sleeved with small arch-shaped sheds, the method is high in survival rate, simple to operate, labor-saving and time-shortening and low in cost, and has feasibility in production.
Description
Technical field
The present invention relates to a kind of booth at high and cold two nether region tetraploid roots of large-flowered skullcap cover small arched shed cuttage and seedling culture method, belong to planting technical field.
Background technology
The tetraploid root of large-flowered skullcap demonstrates clear superiority than the common diploid root of large-flowered skullcap in output, active constituent content etc., has promotion prospect, but the tetraploid root of large-flowered skullcap is bloomed less and late, through observing for many years, have no solid plant, therefore can not breed with seed, vegetative propagation can only be carried out with branch cutting.
Summary of the invention
Be promoted to make the tetraploid root of large-flowered skullcap, this scientific achievement is made to be applied to production, ensure clinical drug safety, the application improves tetraploid root of large-flowered skullcap cuttage raise seedling technique, proposes at high and cold two nether region booth inner sleeve small arched shed cuttage and seedling culture fast breeding techniques.
Technical scheme:
The booth cover small arched shed cuttage and seedling culture method of the high and cold two nether region tetraploid roots of large-flowered skullcap, comprises the steps:
1st step, planting site is built booth, water, dig, apply fertilizer and agricultural chemicals, then in canopy, offer water channel in ground, water channel limit is made little furrow;
2nd step, in the first tenday period of a month in late April to May, take off branch section from maternal plant, carry out cuttage in the little furrow in the 1st step, after sterilization, little furrow build small arched shed, and by real by soil pressure around;
After 3rd step, cuttage 1 ~ 7 day, small arched shed 90% sunshade net hid, and within 7 days, hides later, then small arched shed is taken off film with 50% sunshade net, continue cultivation to seedling.
In the 1st described step, between plantation region height above sea level 1900 ~ 2400m, pH value is 7.0 ~ 9.0, and the soil texture is loam.
In the 1st described step, the wide 40cm of water channel, dark 30cm, each 20 cm of two canal hem widths of water channel.
In the 1st described step, the wide 0.8m of little furrow, long 2.6m, furrow spacing 30cm.
In the 1st described step, apply in fertilising and agricultural chemicals: mu executes well-rotted farmyard manure 2500kg, phosphorus two ammonium 40kg, urea 20kg, phoxim or malicious zinc missible oil 0.5kg, carbendazim or thiophanate methyl 0.5kg.
In the 2nd described step, take off branch section from maternal plant and refer to the segment cutting 5 ~ 7cm length from the eustipes part of maternal plant edible tender branch.
In the 2nd described step, the spacing in the rows 2 ~ 3cm of cuttage, line-spacing 6 ~ 7cm, the degree of depth 2 ~ 3cm.
In the 3rd described step, in booth, intensity of illumination controls at about 80000lx; After cuttage 1 ~ 7 day, in small arched shed, illuminance was at 7000 ~ 8000lx, and after cuttage after 7 days, in small arched shed, illuminance is at 15000 ~ 30000lx.
In the 3rd described step, if late April is to late May cuttage seeding, small arched shed takes off film in 25 days; If 6 ~ early July cuttage seeding, then within 20 days, take off film; If August cuttage seeding, then within 30 days, take off film; If September cuttage seeding, then within 40 days, take off film later.
In the 3rd described step, after cuttage, canopy temperature controls below 35 DEG C daytime, and in small arched shed, temperature controls below 38 DEG C, and 5 ~ 10 cm ground temperature are below 30 DEG C; Night, canopy temperature was more than 10 DEG C, and in small arched shed, temperature controls more than 13 DEG C.
In the 3rd described step, after cuttage, booth humidity controls more than 65%, and small arched shed humidity more than 80%, soil moisture is more than 30%.
beneficial effect
Adopt method provided by the invention, cuttage survival rate on average reaches 96.4%, open country cuttage survival rate is up to 46.7%, greenhouse by solar heat common management cuttage survival rate is up to 51.2%, and adopt small arched shed cuttage and seedling culture in booth, survival rate is high, simple to operate, saving of work and time, cost is low, has feasibility aborning.
Embodiment
Below by embodiment, the present invention is described in further detail.But it will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting scope of the present invention.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, being can by the conventional products of commercial acquisition.
Well-rotted farmyard manure described in the present invention selects one or several the mixing in horse, ox, sheep or pig manure through becoming thoroughly decomposed 30 ~ 40 days gained.
High 25 ~ the 35cm of stem of the tetraploid root of large-flowered skullcap maternal plant in following examples; Leaf is to life, and blade drapes over one's shoulders needle-like; Network of roots is raw, 2 ~ 6, long about 15 cm of root.
embodiment 1
1 selection of land builds canopy
Select between height above sea level 1900 ~ 2400m, pH value is 7.0 ~ 9.0, the soil texture is loam, without foreign material plot such as stones, at late March to early and middle ten days in April, build with bamboo chip or build steelframe booth, simultaneously, at original tetraploid root of large-flowered skullcap maternal plant plot scaffolding epiphragma, apply fertilizer, watering makes its germination and emergence also growth fast ahead of time, the row culture cuttage that sends forth branches is used.
2 whole ground beddings
After booth is built up, water once permeable, after within 3 ~ 4 days, land for building is dry, soil deeptillage, in conjunction with ploughing, mu in booth executes well-rotted farmyard manure 2500kg, phosphorus two ammonium 40kg, urea 20kg, phoxim or malicious zinc missible oil 0.5kg, carbendazim or thiophanate methyl 0.5kg, pick up useless mulch film, stone, tile, preceding crop tangerine stalk, root stubble etc. to the greatest extent, a kind of farm tools of raking is thin, then in the middle of ground, opens a wide 40cm, dark 30cm water channel along booth length, each 20 cm of two canal hem widths, are divided into two pieces by canopy along canopy is long.Wide by 0.8m, the long 2.6m in soil, both sides is made little furrow, furrow spacing 30cm.
3 beta pruning cuttages, take small arched shed
The first tenday period of a month in late April to May, when maternal plant branch grows to 10 ~ 15cm, before being selected at 10 in fine day morning, or after at 5 in afternoon, or select overcast and rainy, the long segment of 5 ~ 7cm is cut from the eustipes part of maternal plant edible tender branch, by spacing in the rows 2 ~ 3cm, line-spacing 6 ~ 7cm, the degree of depth 2 ~ 3cm cuttage is in little furrow, furrow are often inserted, adding 75% tpn 25g disinfectant with 100kg water waters permeable, use No. 8 iron wires immediately, be cut into the long segment of 1m, every furrow 4 ~ 5, curve semicircle and make frame, small arched shed is made with 1.2m plastic mulching, real by soil pressure around small arched shed, prevent gas leakage, then with shading rate be 90% sunshade net hide.
Manage after 4 cuttages
4.1 temperature treatment
After cuttage, canopy temperature controls below 35 DEG C daytime, the measure such as should take regulating tuyere in time when exceeding, pour water, and reduce temperature, in small arched shed, temperature controls below 38 DEG C, and 5 ~ 10 cm ground temperature, below 30 DEG C, should water little water for cooling when exceeding in time.Night, canopy temperature was more than 10 DEG C, and in small arched shed, temperature controls more than 13 DEG C.
4.2 moisture management
After cuttage, booth humidity controls more than 65%, and small arched shed humidity more than 80%, soil moisture is more than 30%.
4.3 intensity of illumination management
After cuttage 1 ~ 7 day, in booth, intensity of illumination was at about 80000lx, and small arched shed 90% sunshade net hides, and in small arched shed, intensity of illumination is at 7000 ~ 8000lx, and within 7 days, later with 50% sunshade net covering, in small arched shed, intensity of illumination is at 15000 ~ 30000lx.
4.4 small arched shed film-uncovering time
Late April, small arched shed took off film in 25 days, enters normal management to late May cuttage seeding, and normal management comprises and weeding out the rank grass at any time, soil moisture see dry see wet, prevention and elimination of disease and pests at any time.
(in addition, if 6 ~ early July cuttage seeding, then within 20 days, take off film, enter normal management; If August cuttage seeding, then within 30 days, take off film, enter normal management; If September cuttage seeding, then within 40 days, take off film later, enter normal management.）
4.5 pest management
Easily powdery mildew is there is after tetraploid root of large-flowered skullcap cuttage seeding enters late July, with 25% triadimefon wetting powder, 800 times of liquid, 500 ~ 800 times of liquid sprayings of 50% carbendazol wettable powder, 500 ~ 600 times of liquid spraying controls of 75% tpn wetting powder after occurring, after August, aphid easily endangers, with ant worm woods, Acetamiprid 2500 times of liquid spraying controls.
reference examples 1
Be with the difference of embodiment 1: adopt greenhouse gardening, do not use small arched shed.Other implantation time, management method are all identical.
reference examples 2
Be with the difference of embodiment 1: during step 4.3 illuminance manages, small arched shed does not adopt sunshade net to hide, and does not control the intensity of illumination of small arched shed, and other way to manage, film-uncovering time etc. are all identical.
That is: after cuttage 1 ~ 7 day, in booth, illuminance was at about 80000lx, small arched shed plastic mulching, and late April, small arched shed took off film in 25 days, enters normal management to late May cuttage seeding.
reference examples 3
Be with the difference of embodiment 1: the time choosing new branch from maternal plant in the 3rd step is the first tenday period of a month in late April to May, and between 12 o'clock to 2 o'clock of fine day, remaining plantation is all identical with management method.
reference examples 4
Be with the difference of embodiment 1: the seedling raising manners not adopting booth, small arched shed, complete outdoor plantation.
reference examples 5
Be with the difference of embodiment 1: in the 3rd step, the position intercepting segment from maternal plant is position between stem, but not the stipes position in embodiment 1.Other cuttage, management method are all identical.
The tetraploid root of large-flowered skullcap of planting in above embodiment and reference examples, all in late October seedling then, investigates the survival rate in whole seedling raising process, single plant yield, content of baicalin.
Wherein, the detection method of content of baicalin adopts HPLC chromatography determination.
Chromatographic condition: Agilent 1100 high performance liquid chromatograph; Chromatographic column: C
18; Mobile phase: methanol-water-phosphoric acid (47:53:0.2); Determined wavelength: 280 nm; Flow velocity: 1.0 ml/min; Column temperature: 25 DEG C.
Prepared by reference substance solution: the scutelloside of vacuum desiccator drying is made into 1ml and contains the solution of 60 μ g and get final product.
Prepared by need testing solution: get tetraploid Baical Skullcap Root (crossing 80 mesh sieves) about 0. 3g, the accurately weighed 70% ethanol 40ml that adds adds hot reflux 3h, let cool, filter, filtrate puts l00ml volumetric flask, with a small amount of 70% ethanol gradation washing container and residue, in the same volumetric flask of washing lotion filter people, add 70% ethanol and be settled to scale, the accurate 0.5ml. that draws adds in 5ml volumetric flask, add methanol constant volume to scale, shake up and get final product.
Accurate absorption test sample, each 10 μ 1 of reference substance solution, inject high performance liquid chromatograph, and record peak area, calculates by external standard method, obtain the percentage composition of scutelloside in dry product.
Table 1
Embodiment 1 and reference examples 1 contrast, and by adding the seedling raising manners of small arched shed in booth, can improve survival rate.Embodiment 1 and reference examples 2 contrast and can find out, small arched shed adopts sunshade net to hide, and can effectively improve survival rate and single plant yield.Embodiment 1 and reference examples 1 contrast, and can finding out, by choosing the time of the new branch of suitable harvesting maternal plant, can improve survival rate.Embodiment 1 is compared with reference examples 5, survival rate is high, this is because the position by controlling to intercept sprig is positioned at eustipes part, the growth of branch can be improved preferably, intercepting sprig survival rate when stipes position is 96.4%, between stem, during position, then the cuttage survival rate of the root of large-flowered skullcap is 72.3%, and stem section cutting part is easily formed hollow, causes disease to contaminate.
Claims (10)
1. the booth cover small arched shed cuttage and seedling culture method of the high and cold two nether region tetraploid roots of large-flowered skullcap, is characterized in that, comprise the steps:
1st step, planting site is built booth, water, dig, apply fertilizer and agricultural chemicals, then in canopy, offer water channel in ground, water channel limit is made little furrow;
2nd step, in the first tenday period of a month in late April to May, take off branch section from maternal plant, carry out cuttage in the little furrow in the 1st step, after sterilization, little furrow build small arched shed, and by real by soil pressure around;
After 3rd step, cuttage 1 ~ 7 day, small arched shed 90% sunshade net hid, and within 7 days, hide with 50% sunshade net, then district's small arched shed took off film later, continued cultivation to seedling.
2. the booth cover small arched shed cuttage and seedling culture method of the high and cold two nether region tetraploid roots of large-flowered skullcap according to claim 1, it is characterized in that: in the 1st described step, between plantation region height above sea level 1900 ~ 2400m, pH value is 7.0 ~ 9.0, and the soil texture is loam.
3. the cover of the booth at high and cold two nether region tetraploid roots of large-flowered skullcap small arched shed cuttage and seedling culture method according to claim 1, is characterized in that: in the 1st described step, the wide 40cm of water channel, dark 30cm, each 20 cm of two canal hem widths of water channel.
4. the cover of the booth at high and cold two nether region tetraploid roots of large-flowered skullcap small arched shed cuttage and seedling culture method according to claim 1, is characterized in that: in the 1st described step, the wide 0.8m of little furrow, long 2.6m, furrow spacing 30cm.
5. the cover of the booth at high and cold two nether region tetraploid roots of large-flowered skullcap small arched shed cuttage and seedling culture method according to claim 1, it is characterized in that: in the 1st described step, apply in fertilising and agricultural chemicals: mu executes well-rotted farmyard manure 2500kg, phosphorus two ammonium 40kg, urea 20kg, phoxim or malicious zinc missible oil 0.5kg, carbendazim or thiophanate methyl 0.5kg.
6. the cover of the booth at high and cold two nether region tetraploid roots of large-flowered skullcap small arched shed cuttage and seedling culture method according to claim 1, it is characterized in that: in the 2nd described step, take off branch section from maternal plant and refer to the segment cutting 5 ~ 7cm length from the eustipes part of maternal plant edible tender branch.
7. the cover of the booth at high and cold two nether region tetraploid roots of large-flowered skullcap small arched shed cuttage and seedling culture method according to claim 1, is characterized in that: in the 2nd described step, the spacing in the rows 2 ~ 3cm of cuttage, line-spacing 6 ~ 7cm, the degree of depth 2 ~ 3cm.
8. the cover of the booth at high and cold two nether region tetraploid roots of large-flowered skullcap small arched shed cuttage and seedling culture method according to claim 1, is characterized in that: in the 3rd described step, in booth, intensity of illumination controls at about 80000lx; After cuttage 1 ~ 7 day, in small arched shed, intensity of illumination was at 7000 ~ 8000lx, and after cuttage after 7 days, in small arched shed, intensity of illumination is at 15000 ~ 30000lx.
9. the cover of the booth at high and cold two nether region tetraploid roots of large-flowered skullcap small arched shed cuttage and seedling culture method according to claim 1, it is characterized in that: in the 3rd described step, if late April is to late May cuttage seeding, small arched shed takes off film in 25 days; If 6 ~ early July cuttage seeding, then within 20 days, take off film; If August cuttage seeding, then within 30 days, take off film; If September cuttage seeding, then within 40 days, take off film later.
10. the cover of the booth at high and cold two nether region tetraploid roots of large-flowered skullcap small arched shed cuttage and seedling culture method according to claim 1, it is characterized in that: in the 3rd described step, after cuttage, canopy temperature controls below 35 DEG C daytime, in small arched shed, temperature controls below 38 DEG C, and 5 ~ 10 cm ground temperature are below 30 DEG C; Night, canopy temperature was more than 10 DEG C, and in small arched shed, temperature controls more than 13 DEG C; In the 3rd described step, after cuttage, booth humidity controls more than 65%, and small arched shed humidity more than 80%, soil moisture is more than 30%.
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| CN201510099642.6A CN104620837B (en) | 2015-03-07 | 2015-03-07 | The booth set small arched shed cuttage and seedling culture method of high and cold two nether region tetraploid Radix Scutellariaes |
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| CN201510099642.6A CN104620837B (en) | 2015-03-07 | 2015-03-07 | The booth set small arched shed cuttage and seedling culture method of high and cold two nether region tetraploid Radix Scutellariaes |
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Cited By (2)
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| CN105993716A (en) * | 2016-05-28 | 2016-10-12 | 林晶 | Use method for volume-changeable awning-in-greenhouse of plastic-cover greenhouse |
| CN108207516A (en) * | 2018-01-23 | 2018-06-29 | 青海省农林科学院 | A kind of technology for cultivating early maturing cabbage production of hybrid seeds seedling in extremely frigid zones using heliogreenhouse |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108207516A (en) * | 2018-01-23 | 2018-06-29 | 青海省农林科学院 | A kind of technology for cultivating early maturing cabbage production of hybrid seeds seedling in extremely frigid zones using heliogreenhouse |
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