CN104365406A - Simple and efficient cultivation method convenient to implement for sugarcane seed seedlings - Google Patents
Simple and efficient cultivation method convenient to implement for sugarcane seed seedlings Download PDFInfo
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- CN104365406A CN104365406A CN201410659678.0A CN201410659678A CN104365406A CN 104365406 A CN104365406 A CN 104365406A CN 201410659678 A CN201410659678 A CN 201410659678A CN 104365406 A CN104365406 A CN 104365406A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses a simple and efficient cultivation method convenient to implement for sugarcane seed seedlings. According to the cultivation method, the number of set of equipment required by steam autoclaving sterilization or dry heat sterilization on a culture medium is reduced, and energy and labor are saved; meanwhile, bagasse is fully used as a part of the medium, and therefore the seedlings can quickly absorb required effective nutritional ingredients; besides, the cultivation method is simple in operation process and convenient to implement, low in cultivation cost, high in seedling survival rate, capable of shortening the cultivation time and high in operability and can be applied and popularized in sugarcane seedling cultivation in any sugarcane area.
Description
Technical field
The invention belongs to breed of crop technical field, be specifically related to a kind of simple and effective sugarcane seedling from seed breeding method.
Background technology
Sugarcane is the main sugar [yielding of China, also be first generation biofuel crop, the seed selection of current new variety of sugarcane is still and obtains seed by sexual hybridization, cultivating seeds is gone out seedling, again seedling is implanted in land for growing field crops, forms through test seed selections such as hybrid nursery, selective nursery, qualification garden, reserve ratio, varietal yield test, regional tests.Sugarcane belongs to allopolyploid plant, and genetic background is very complicated, and breeder needs sowing several ten thousand therefrom to carry out seed selection to millions of seedling from seed every year.Because sugarcane seed is tiny, thousand grain weigth is extremely light, the outer attached fine hair of seed, include nutrient few, bud ratio is affected by environment fairly obvious, can not directly at field seeding, first will carry out seedling breeding, therefore the way to cultivate of cane seedlings directly affects the seed selection of new variety of sugarcane.
Due to the character of the uniqueness of sugarcane seed, the process such as the cultivation of seedling generally needs through germination test, sows, heels in, field planting.The soil of sowing needs to carry out steam autoclave sterilization or dry heat sterilization, when seedling grows to 6 ~ 10 cm, need heel in seedbed by seedling, guarantee that seedling has enough growing spaces.Because cane seedlings seeding quantity is comparatively large, need a large amount of disinfecting soil and needs are a large amount of manually carries out seedling at short notice and heel in, so not only consuming timely to take a lot of work, and cost is very high.How simple and effective cultivates sugarcane seedling from seed is one of breed breeding key issue in the urgent need to address.
Summary of the invention
The object of the invention is to overcome above-mentioned the deficiencies in the prior art, a kind of simple and effective sugarcane seedling from seed breeding method is provided, solve loaded down with trivial details soil disinfection work and seedling when conventional sugarcane seedling from seed is cultivated and heel in required a large amount of labours, simplify the techniqueflow that conventional sugarcane seedling from seed is cultivated, improve the efficiency of seedling breeding, save a large amount of man power and material.
Above-mentioned purpose of the present invention is achieved by following technical solution:
A kind of simple and effective sugarcane seedling from seed breeding method, comprises the steps:
S1. set up a breeding printing opacity greenhouse, make and cultivate wooden case;
S2. culture matrix is prepared: by mold and bagasse by weight 2 ~ March of 2 ~ 3:1 mixed culture fermentation, stir 2 ~ 3 times afterwards, make the weed seed fast germination in soil and remove weeds, then sieved by the soil after fermentation and add the obtained culture matrix of phosphate fertilizer mixing, the soil after described fermentation mixes by weight 25 ~ 35:1 with phosphate fertilizer;
S3. sow: obtained culture matrix is positioned over and cultivates in wooden case, culture matrix surface sowing in cultivation wooden case;
S4. temperature control moisturizing: after having sowed, controlling warm indoor temperature is 26 ~ 33 DEG C, and relative moisture is more than 85%, notes water spray every day, keeps cultivating the moistening but not ponding of wooden case;
S5. growth promotion: using carbendazim wettable powder and the culture matrix of 50% to dilute to mix to mass percent is 0.5 ~ 1% obtain the first bactericidal agent, using thiophanate methyl wettable powder and the culture matrix of 50% to dilute to mix to mass percent is 0.05% ~ 0.1% obtain the second bactericidal agent, when seed sprouting and after growing the seedling of 3 ~ 4 leaves, alternate cycles uses the first bactericidal agent and the second bactericidal agent to apply 1 sterilization weekly;
S6. hardening: after seedling grows 5 ~ 6 leaves, shifts out greenhouse by cultivation wooden case and carries out hardening; If run into bad weather (heavy rain or heavy rain), need in time cultivation wooden case to be transferred back in greenhouse;
S7. to apply fertilizer leaf-cutting: hardening is after one week, be that the composite fertilizer of 1:0.5 ~ 1:1 ~ 2 adds water and is mixed with nutrient solution by nitrogen phosphoris and potassium fertilizer ratio, spraying weekly a concentration is the nutrient solution of 0.1%, after 2 ~ 3 weeks, changing into and spraying weekly a concentration is the nutrient solution of 0.5%, until when seedling grows to 30 more than cm, field planting land for growing field crops stops fertilising, and period, leaf-cutting was once weekly.
The present invention utilizes mold and bagasse fermentation as culture matrix, after stirring repeatedly, weed seed in soil all germinates removal substantially, which save equipment required when matrix needs steam autoclave sterilization or dry heat sterilization, the energy and labour etc. are saved, make full use of bagasse as fraction matrix simultaneously, be conducive to seedling and absorb required effective nutrient component fast.
Bagasse of the present invention is conventional bagasse, can get the sugarcane stem in sugarcane maturing stage, sugarcane leaf, the sugarcane root and stem of certain plants as raw material carry out pulverizing obtain, particularly, the bagasse stayed in the bagasse or sugar refinery production that in sugarcane research institute, cane stalk sugar stays after detecting squeezing can directly be adopted.
Preferably, the concrete technology of step S2 is mold and bagasse are mixed by weight 2 ~ 3:1 ratio, mixture employing is watered without the clean water polluted and is carried out pile fermentation, irrigation amount controls the water irrigating and does not outflow, avoid causing nutrient loss, on stack retting thing, covered with plastic film heat and moisture preserving, fully ferments for 2 ~ March; After having fermented, remove cover film, week about matrix is stirred and water 1 time, stir 2 ~ 3 times continuously, make the weed seed fast germination in soil and remove weeds, then sieved by the soil after fermentation and add the obtained culture matrix of phosphate fertilizer mixing, the soil after described fermentation and phosphate fertilizer by weight 25 ~ 35:1 mix, described in the order number that sieves be 10 ~ 14 orders.
As a kind of preferred version, the concrete operations sowed described in step S3 are for being dispersed evenly to culture matrix surface with thin thin bamboo strips used for weaving by loose for the bat of sugarcane fringe, with sprayer water spray, flower fringe seed is sprayed on cultivation wooden case, then cover one deck culture matrix on flower fringe the surface of the seed.As preferred further, the culture matrix thickness that described colored fringe the surface of the seed covers is 0.1 ~ 0.2cm, flower fringe the surface of the seed covers medium mass-energy and avoids seed to be dispelled movement, also can temperature control moisturizing better, also there is good permeation functions simultaneously, promote the long seedling of seed sprouting.
In step S5 of the present invention, after seedling grows 3 ~ 4 leaves, because seed nutrient consumption is complete, root leaf is also not vigorous, premunition is poor, add that matrix is in moist environment for a long time, thus need the carbendazim of service property (quality) percentage 0.5 ~ 1% or service property (quality) percentage be 0.05 ~ 0.1% thiophanate methyl interval spray, can significantly improve prevention seedling stage pine root fungus harm.
In step S7 of the present invention, the nitrogen-phosphorus-potassium compound fertilizer getting special ratios configures nutrient solution and unconventional selection, because sugarcane is a kind of happiness potassium crop, to the requirement of potassium greatly and very responsive, the growth of cane seedlings is obviously affected during potassium deficiency, behind field planting land for growing field crops, survival rate declines, the ratio of potash fertilizer should be controlled well when therefore preparing nutrient solution, test through great many of experiments, adopt nitrogen phosphoris and potassium fertilizer ratio to be that the cane seedlings growth that 1:0.5 ~ 1:1 ~ 2 composite fertilizer cultivates is best, after field planting, land for growing field crops survival rate is high.
As a kind of preferred version, when sowing in step S3, according to sugarcane hybridization flower Spike sprouting rate, sow according to the quantity of each cultivation wooden case 250 ~ 300 strain seedling, growing space is made just to be applicable to growth of seedling, saving is heeled in needs man power and material greatly, avoids heeling in the death of seedling in process and improves growth rate.
Preferably, described leaf-cutting is cut off by the blade hung down loosely with apart from the leaf heart 2 cm with upper part young leaves, to promote that strong sprout strengthens root, improves field planting land for growing field crops survival rate.
As a kind of preferred version, making the specification of cultivating wooden case in step S1 is long 60 cm, wide 40 cm, high 8 cm.As further preferred version, in sugarcane seedling from seed cultivating process, described cultivation wooden case is positioned on four wheel barrows, both can facilitate mobile seedling hardening or avoid bad weather to the injury of seedling, make again seedling management easy to operate, described four wheel barrows are prepared by iron plate and steel pipe, and specification is long 200 cm, wide 100 cm, high 90 cm, each carriage can be placed two rows and cultivate wooden case, and often 5 casees are put in discharge.
Compared with prior art, the present invention has following beneficial effect:
(1) breeding method of the present invention saves equipment required when culture matrix needs steam autoclave sterilization or dry heat sterilization, the energy and labour etc. are saved, make full use of bagasse as fraction matrix simultaneously, be conducive to seedling and absorb required effective nutrient component fast.
(2) breeding method operating process of the present invention is simple and convenient, cultivate old cheap, seedling survival rate is high, shortens the cultivation time simultaneously, workable, and cane seedlings in any sugarcane district all can be applicable in cultivating.
Embodiment
Below in conjunction with specific embodiment, the present invention is further explained, but embodiments of the present invention is not limited in any way.Unless stated otherwise, involved in embodiment reagent, method are the conventional reagent in this area and method.
embodiment 1
(1) a breeding greenhouse is set up: greenhouse addressing is in more spacious, around block without tall and big trees or crops, position is easily irrigated in traffic, sets up long-term glass greenhouse or brief plastic tunnel printing opacity greenhouse, Greenhouse building area is determined according to seedling sowing scale, is specially 320m
2sow about 120,000 seedlings.
(2) cultivation wooden case and four wheel barrows are made: use plank to make rectangle and cultivate wooden case, specification is long 60 cm, wide 40 cm, high 8 cm, iron plate and steel pipe is utilized to prepare four wheel barrows, specification is long 200 cm, wide 100 cm, high 90 cm, each carriage can be placed two rows and cultivate wooden case, and often 5 casees are put in discharge.
(3) culture matrix is prepared: mold and bagasse are mixed by weight 2 ~ 3:1 ratio, mixture employing is watered without the clean water polluted and is carried out pile fermentation, irrigation amount controls the water irrigating and does not outflow, avoid causing nutrient loss, on stack retting thing, covered with plastic film heat and moisture preserving, fully ferments for 2 ~ March; After having fermented, remove cover film, week about matrix is stirred and water 1 time, stir 2 ~ 3 times continuously, make the weed seed fast germination in soil and remove weeds, then sieved by the soil after fermentation and add the obtained culture matrix of phosphate fertilizer mixing, the soil after described fermentation mixes by weight 30:1 with phosphate fertilizer, the described order number sieved is 10 ~ 14 orders, and described bagasse is the sugarcane stem in sugarcane maturing stage, sugarcane leaf, the sugarcane root and stem of certain plants carry out pulverizing as raw material and obtain.
(4) sow: sow front water and greenhouse is cleaned up, the culture matrix that step (3) is obtained is placed on and cultivates in wooden case, compacting leveling a little, the thickness of culture matrix is made to be about 1cm, wooden case is numbered, is positioned in order (2 rows placed by each car, often arrange 5 casees) on four wheel barrows.Select the weather good time, according to sugarcane hybridization flower Spike sprouting rate, sow on culture matrix surface according to the quantity of each cultivation wooden case 250 ~ 300 strain seedling, be specially and be dispersed evenly to culture matrix surface with thin thin bamboo strips used for weaving by loose for the bat of sugarcane fringe, with sprayer water spray, flower fringe seed is sprayed on cultivation wooden case, then cover the thick culture matrix of one deck 0.1 ~ 0.2 cm on flower fringe the surface of the seed.
(5) temperature control moisturizing: after having sowed, controlling warm indoor temperature is 26 ~ 33 DEG C, and relative moisture is more than 85%, notes water spray every day, keeps cultivating the moistening but not ponding of wooden case.
(6) growth promotion: sow 2 ~ 3 days seeds and just start rudiment, keep the temperature and humidity in greenhouse, when after the seedling growing 3 ~ 4 leaves, because seed nutrient consumption is complete, root leaf is also not vigorous, premunition is poor, adds that matrix is in moist environment for a long time, weekly the carbendazim of service property (quality) percentage 0.5 ~ 1% or service property (quality) percentage be 0.05 ~ 0.1% thiophanate methyl interval spray.
(7) hardening: after seedling grows 5 ~ 6 leaves, shifts out greenhouse by cultivation wooden case and carries out hardening; If run into bad weather (heavy rain or heavy rain), need in time cultivation wooden case to be transferred back in greenhouse.
(8) fertilising leaf-cutting: hardening is after a week, be that the composite fertilizer of 1:1:2 adds water and is mixed with nutrient solution by nitrogen phosphoris and potassium fertilizer ratio, spraying weekly a mass concentration is the nutrient solution of 0.1%, after 2 ~ 3 weeks, changing into and spraying weekly a concentration is the nutrient solution of 0.5%, period weekly leaf-cutting once, cut off by the blade hung down loosely with apart from the leaf heart 2 cm with upper part young leaves, to promote strengthen root strong sprout, improve field planting land for growing field crops survival rate.Can by seedling direct planting in large Tanaka when seedling grows to 30 more than cm.
embodiment 2
Key step is identical with embodiment 1, and distinctive points is that in the composite fertilizer adopted in step (7), nitrogen phosphoris and potassium fertilizer ratio is 1:1:1.
embodiment 3
Key step is identical with embodiment 1, and distinctive points is that in the composite fertilizer adopted in step (7), nitrogen phosphoris and potassium fertilizer ratio is 1:0.5:2.
comparative example 1
Key step is identical with embodiment 1, and distinctive points is culture matrix in step (3) by mold and bagasse in directly mixing obtained by weight 2 ~ 3:1 ratio.
comparative example 2
Key step is identical with embodiment 1, and distinctive points is that in the composite fertilizer adopted in step (7), nitrogen phosphoris and potassium fertilizer ratio is 2:1:1.
It is as shown in table 1 below that result is transplanted in the seedling breeding result of embodiment 1 ~ 3 and comparative example 1 ~ 2 and land for growing field crops, as can be seen from Table 1, adopt breeding method of the present invention to cultivate sugarcane seedling from seed and can shorten the cultivation time compared with conventional method, seedling growth is neat, sturdy, survival rate after the transplanting of land for growing field crops is higher, growth is more normal, and overall productivity is also higher.As can also be seen from Table 1, the growth conditions that embodiment 1 is cultivated after the seedling and land for growing field crops transplanting thereof obtained is optimum, survival rate is the highest, and the proportioning composition further illustrating composite fertilizer is very large to the cultivation qualitative effects of cane seedlings, and the composition and ratio of composite fertilizer is not the ordinary skill in the art.
Table 1:
Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | |
Sample size | 2500 | 2500 | 2500 | 2500 | 2500 |
Cultivation time/week | 8 | 8 | 8 | 10 | 9 |
Cultivate seedling plant height (cm) after 8 weeks | 15.5 | 15.1 | 14.4 | 11.2 | 12.7 |
Cultivate seedling stem footpath (cm) after 8 weeks | 0.34 | 0.31 | 0.32 | 0.24 | 0.27 |
Land for growing field crops transplanting success | 98.8% | 95.3% | 97.0% | 85.4% | 88.7% |
Field planting is plant height (cm) after 6 months | 213.7 | 209.3 | 211.4 | 198.5 | 204.3 |
Stem footpath (cm) after field planting June | 2.35 | 2.31 | 2.30 | 2.17 | 2.18 |
Output (ton/hectare) after field planting June | 65.37 | 58.60 | 60.75 | 48.58 | 48.05 |
Claims (9)
1. a simple and effective sugarcane seedling from seed breeding method, is characterized in that, comprise the steps:
S1. set up a breeding printing opacity greenhouse, make and cultivate wooden case;
S2. culture matrix is prepared: by mold and bagasse by weight 2 ~ March of 2 ~ 3:1 mixed culture fermentation, stir 2 ~ 3 times afterwards, make the weed seed fast germination in soil and remove weeds, then sieved by the soil after fermentation and add the obtained culture matrix of phosphate fertilizer mixing, the soil after described fermentation mixes by weight 25 ~ 35:1 with phosphate fertilizer;
S3. sow: obtained culture matrix is positioned over and cultivates in wooden case, culture matrix surface sowing in cultivation wooden case;
S4. temperature control moisturizing: after having sowed, controlling warm indoor temperature is 26 ~ 33 DEG C, and relative moisture is more than 85%, and every day sprays water, and keeps cultivating the moistening but not ponding of wooden case;
S5. growth promotion: using carbendazim wettable powder and the culture matrix of 50% to dilute to mix to mass percent is 0.5 ~ 1% obtain the first bactericidal agent, using thiophanate methyl wettable powder and the culture matrix of 50% to dilute to mix to mass percent is 0.05% ~ 0.1% obtain the second bactericidal agent, when seed sprouting and after growing the seedling of 3 ~ 4 leaves, alternate cycles uses the first bactericidal agent and the second bactericidal agent to apply 1 sterilization weekly;
S6. hardening: after seedling grows 5 ~ 6 leaves, shifts out greenhouse by cultivation wooden case and carries out hardening;
S7. to apply fertilizer leaf-cutting: hardening is after one week, be that the composite fertilizer of 1:0.5 ~ 1:1 ~ 2 adds water and is mixed with nutrient solution by nitrogen phosphoris and potassium fertilizer ratio, spraying weekly a concentration is the nutrient solution of 0.1%, after 2 ~ 3 weeks, changing into and spraying weekly a concentration is the nutrient solution of 0.5%, until when seedling grows to 30 more than cm, field planting land for growing field crops stops fertilising, and period, leaf-cutting was once weekly.
2. breeding method according to claim 1, it is characterized in that, the concrete technology of mixed culture fermentation described in step S2 is mold and bagasse are mixed by weight 2 ~ 3:1 ratio, mixture employing water waters and carries out pile fermentation, on stack retting thing, covered with plastic film heat and moisture preserving, fully ferments for 2 ~ March.
3. breeding method according to claim 1, it is characterized in that, the concrete operations sowed described in step S3 are for being dispersed evenly to culture matrix surface with thin thin bamboo strips used for weaving by loose for the bat of sugarcane fringe, with sprayer water spray, flower fringe seed is sprayed on cultivation wooden case, then cover one deck culture matrix on flower fringe the surface of the seed.
4. breeding method according to claim 3, it is characterized in that, the culture matrix thickness that described colored fringe the surface of the seed covers is 0.1 ~ 0.2cm.
5. breeding method according to claim 1, is characterized in that, when sowing in step S3, according to sugarcane hybridization flower Spike sprouting rate, sows according to the quantity of each cultivation wooden case 250 ~ 300 strain seedling.
6. breeding method according to claim 1, is characterized in that, described leaf-cutting is cut off by the blade hung down loosely with apart from the leaf heart 2 cm with upper part young leaves.
7. breeding method according to claim 1, is characterized in that, making the specification of cultivating wooden case in step S1 is long 60 cm, wide 40 cm, high 8 cm.
8. breeding method according to claim 7, it is characterized in that, described cultivation wooden case is positioned on four wheel barrows.
9. breeding method according to claim 8, is characterized in that, described four wheel barrows are prepared by iron plate and steel pipe, and specification is long 200 cm, wide 100 cm, high 90 cm, and each carriage can be placed two rows and cultivate wooden case, and often 5 casees are put in discharge.
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Cited By (7)
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CN105027933A (en) * | 2015-08-13 | 2015-11-11 | 云南省农业科学院甘蔗研究所 | Method for improving survival rate of young seedlings of sugarcane seedlings |
CN105191610A (en) * | 2015-08-24 | 2015-12-30 | 太仓市丰缘农场专业合作社 | Method for high-yield planting of sugarcanes |
CN105613003A (en) * | 2016-01-08 | 2016-06-01 | 广州甘蔗糖业研究所 | Seedling breeding method capable of improving breeding efficiency of sugarcane seedlings and application of seedling breeding method |
CN105660099B (en) * | 2016-01-12 | 2018-05-15 | 广西南亚热带农业科学研究所 | A kind of culturing sugarcane seedlings method |
CN110036861A (en) * | 2019-05-20 | 2019-07-23 | 云南省农业科学院甘蔗研究所 | A kind of breeding method of cane seedlings |
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