CN104277988A - Candida chromogenic culture medium and application thereof - Google Patents
Candida chromogenic culture medium and application thereof Download PDFInfo
- Publication number
- CN104277988A CN104277988A CN201410512386.4A CN201410512386A CN104277988A CN 104277988 A CN104277988 A CN 104277988A CN 201410512386 A CN201410512386 A CN 201410512386A CN 104277988 A CN104277988 A CN 104277988A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- candida
- bromcresol purple
- broth base
- substratum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
Abstract
The invention relates to the field of microorganisms and particularly relates to a candida chromogenic culture medium. The culture medium comprises the following components: a bromcresol purple broth base, 2.0g/L sodium hydrogen phosphate, 2.0g/L dipotassium phosphate, 10.0g/L glycine, 0.5g/L potassium chloride, 2.5g/L chloramphenicol, 15.0g/L agar and 0.1g/L chromogenic reagent, and the pH value of the culture medium is 7.2-7.4. According to the candida chromogenic culture medium, each liter of the bromcresol purple broth base contains 10.0g of tryptone, 3.0g of beef extract powder, 5.0g of sodium chloride, 0.04g of bromcresol purple and the balance of distilled water. The culture medium provided by the invention is simple in formula, convenient in preparation operation, low in cost, beneficial to popularization and application and convenient to observe and analyze; and bacteria are rapid in growth and low in mutation probability.
Description
Technical field
The present invention is microorganism field, particularly a kind of Candida chromogenic medium and application thereof.
Background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Can't infer from the ultimate principle of biochemical reaction completely and calculate the culture medium prescription of applicable a certain bacterial classification at present, the basic theories of biological chemistry, cytobiology, microbiology etc. can only be used, with reference to the empirical formula being comparatively applicable to a certain class bacterial classification that forefathers use, again in conjunction with the characteristic of bacterial classification used and product, adopt the small-sized fermentation equipment such as shaking flask, glass pot, select the substratum be comparatively applicable to according to certain experimental design and experimental technique.
The basic step of substratum design is:
Some problems that must consider when determining according to the experience of forefathers and medium component, tentatively determine possible medium component
Medium component the most suitable is finally determined by single factor experiment
After medium component is determined, remaining issues is exactly the suitableeest concentration of each composition, because medium component is a lot, often adopts some rational experimental design methods for reducing experiment number.These experiments, often based on factorial experiment, comprise homogeneous design, orthogonal, response surface analysis etc.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of Candida chromogenic medium and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of Candida chromogenic medium, the component of described substratum comprises bromcresol purple broth base, 2.0g/L Sodium phosphate dibasic, 2.0g/L dipotassium hydrogen phosphate, 10.0g/L glycine, 0.5g/L Repone K, 2.5g/L paraxin, 15.0g/L agar, 0.1g developer, pH value is 7.2-7.4.
On the basis of above scheme, described Candida chromogenic medium, described bromcresol purple broth base is that all the other are distilled water containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base.
The invention also discloses a kind of application of Candida chromogenic medium, for candidiasis separation andpreconcentration, Candida albicans is aobvious green.
Take this product 9.6g (can expand in the ratio of 4.8g/100ml or reduce), add in 200ml distilled water or purified water, heating for dissolving does not stop to stir, and boils and does not exceed 1 minute.Substratum water-bath after heating is cooled to 45-50 DEG C, shakes up lightly, impouring sterilized petri dishes (the 15ml/ ware of 9cm, the 10ml/ ware of 7cm), after agar solidifies completely, upside down 0.5-1 hour in the incubator of 36 DEG C ± 1 DEG C, for subsequent use.
Attention: the substratum prepared must keep surface drying, can in the incubator of 37 DEG C upside down 1-2 hour.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of Candida chromogenic medium, the component of described substratum comprises bromcresol purple broth base, 2.0g/L Sodium phosphate dibasic, 2.0g/L dipotassium hydrogen phosphate, 10.0g/L glycine, 0.5g/L Repone K, 2.5g/L paraxin, 15.0g/L agar, 0.1g developer, pH value is 7.2-7.4.
Described Candida chromogenic medium, described bromcresol purple broth base is that all the other are distilled water containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base.
Take this product 9.6g (can expand in the ratio of 4.8g/100ml or reduce), add in 200ml distilled water or purified water, heating for dissolving does not stop to stir, and boils and does not exceed 1 minute.Substratum water-bath after heating is cooled to 45-50 DEG C, shakes up lightly, impouring sterilized petri dishes (the 15ml/ ware of 9cm, the 10ml/ ware of 7cm), after agar solidifies completely, upside down 0.5-1 hour in the incubator of 36 DEG C ± 1 DEG C, for subsequent use.
Attention: the substratum prepared must keep surface drying, can in the incubator of 37 DEG C upside down 1-2 hour.
Cultivate 48 hours at 25-30 DEG C:
| Quality-control strains | Growing state | Colony colour |
| Candida albicans | +++ | Blue-greenish colour |
| Oidium tropicale | +++ | Pewter, electric blue |
| Gram Rou Shi candidiasis | +++ | Pink |
| Candida glabrata | +++ | Purple |
| Other candidiasis | +++ | White |
| Intestinal bacteria | - | - |
Claims (3)
1. a Candida chromogenic medium, it is characterized in that the component of described substratum comprises bromcresol purple broth base, 2.0g/L Sodium phosphate dibasic, 2.0g/L dipotassium hydrogen phosphate, 10.0g/L glycine, 0.5g/L Repone K, 2.5g/L paraxin, 15.0g/L agar, 0.1g developer, pH value is 7.2-7.4.
2. Candida chromogenic medium according to claim 1, it is characterized in that described bromcresol purple broth base is for containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base, all the other are distilled water.
3. an application for Candida chromogenic medium according to claim 1, is characterized in that for candidiasis separation andpreconcentration, and Candida albicans is aobvious green.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410512386.4A CN104277988A (en) | 2014-09-29 | 2014-09-29 | Candida chromogenic culture medium and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410512386.4A CN104277988A (en) | 2014-09-29 | 2014-09-29 | Candida chromogenic culture medium and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104277988A true CN104277988A (en) | 2015-01-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410512386.4A Pending CN104277988A (en) | 2014-09-29 | 2014-09-29 | Candida chromogenic culture medium and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104277988A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101886111A (en) * | 2010-06-11 | 2010-11-17 | 郑州安图绿科生物工程有限公司 | Fungus rapid culture, identification and susceptibility detection kit and detection method thereof |
| CN101948902A (en) * | 2010-09-10 | 2011-01-19 | 中国检验检疫科学研究院 | Candida chromogenic medium, detection kit and detection method |
| CN102605037A (en) * | 2012-03-06 | 2012-07-25 | 刘艳 | Candida selection medium for stool specimens and preparation method thereof |
| CN103397072A (en) * | 2012-03-06 | 2013-11-20 | 刘艳 | Candida selective culture medium for feces specimen and preparation method thereof |
| CN104651249A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Candida chromogenic medium and use thereof |
-
2014
- 2014-09-29 CN CN201410512386.4A patent/CN104277988A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101886111A (en) * | 2010-06-11 | 2010-11-17 | 郑州安图绿科生物工程有限公司 | Fungus rapid culture, identification and susceptibility detection kit and detection method thereof |
| CN101948902A (en) * | 2010-09-10 | 2011-01-19 | 中国检验检疫科学研究院 | Candida chromogenic medium, detection kit and detection method |
| CN102605037A (en) * | 2012-03-06 | 2012-07-25 | 刘艳 | Candida selection medium for stool specimens and preparation method thereof |
| CN103397072A (en) * | 2012-03-06 | 2013-11-20 | 刘艳 | Candida selective culture medium for feces specimen and preparation method thereof |
| CN104651249A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Candida chromogenic medium and use thereof |
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Application publication date: 20150114 |