CN104237522B - 脂联素含量检测试剂盒及其制备方法 - Google Patents
脂联素含量检测试剂盒及其制备方法 Download PDFInfo
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- CN104237522B CN104237522B CN201210508749.8A CN201210508749A CN104237522B CN 104237522 B CN104237522 B CN 104237522B CN 201210508749 A CN201210508749 A CN 201210508749A CN 104237522 B CN104237522 B CN 104237522B
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Classifications
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
稀释倍数 | 测量值1 | 测量值2 | 测量均值 | 理论值 | 线性偏差(%) |
0.1 | 902 | 898 | 900 | 896 | 0.44 |
0.2 | 1794 | 1796 | 1795 | 1792 | 0.17 |
0.3 | 2671 | 2674 | 2672.5 | 2688 | -0.58 |
0.4 | 3602 | 3585 | 3593.5 | 3584 | 0.26 |
0.5 | 4452 | 4454 | 4453 | 4480 | -0.61 |
0.6 | 5405 | 5386 | 5395.5 | 5376 | 0.36 |
0.7 | 6314 | 6298 | 6306 | 6272 | 0.54 |
0.8 | 7164 | 7146 | 7155 | 7168 | -0.18 |
0.9 | 8076 | 8088 | 8082 | 8064 | 0.22 |
1.0 | 9056 | 8814 | 8935 | 8960 | -0.28 |
稀释倍数 | 测量值1 | 测量值2 | 测量均值 | 理论值 | 线性偏差(%) |
0.1 | 375 | 386 | 380.5 | 398.6 | -4.76 |
0.2 | 765 | 779 | 772 | 797.2 | -3.26 |
0.3 | 1059 | 1056 | 1057.5 | 1195.8 | -13.08 |
0.4 | 1508 | 1498 | 1503 | 1594.4 | -6.08 |
0.5 | 1902 | 1895 | 1898.5 | 1993 | -4.98 |
0.6 | 2401 | 2411 | 2406 | 2391.6 | 0.6 |
0.7 | 2706 | 2685 | 2695.5 | 2790.2 | -3.51 |
0.8 | 3256 | 3234 | 3245 | 3188.8 | 1.73 |
0.9 | 3605 | 3614 | 3609.5 | 3587.4 | 0.61 |
1.0 | 4128 | 4206 | 4167 | 3986 | 4.34 |
稀释倍数 | 测量值1 | 测量值2 | 测量均值 | 理论值 | 线性偏差(%) |
0.1 | 221 | 223 | 222 | 218.1 | 1.76 |
0.2 | 440 | 441 | 440.5 | 436.2 | 0.98 |
0.3 | 674 | 672 | 673 | 654.3 | 2.78 |
0.4 | 865 | 869 | 867 | 872.4 | -0.62 |
0.5 | 1085 | 1083 | 1084 | 1090.5 | -0.6 |
0.6 | 1296 | 1293 | 1294.5 | 1308.6 | -1.09 |
0.7 | 1520 | 1524 | 1522 | 1526.7 | -0.31 |
0.8 | 1751 | 1753 | 1752 | 1744.8 | 0.41 |
0.9 | 1971 | 1965 | 1968 | 1962.9 | 0.26 |
1.0 | 2196 | 2194 | 2195 | 2181 | 0.64 |
稀释倍数 | 测量值1 | 测量值2 | 测量均值 | 理论值 | 线性偏差(%) |
0.1 | 345 | 358 | 351.5 | 335.6 | 4.52 |
0.2 | 745 | 732 | 738.5 | 671.2 | 9.11 |
0.3 | 1102 | 1098 | 1100 | 1006.8 | 8.47 |
0.4 | 1414 | 1425 | 1419.5 | 1342.4 | 5.43 |
0.5 | 1732 | 1742 | 1737 | 1678 | 3.4 |
0.6 | 2156 | 2186 | 2171 | 2013.6 | 7.25 |
0.7 | 2432 | 2465 | 2448.5 | 2349.2 | 4.06 |
0.8 | 2738 | 2714 | 2726 | 2684.8 | 1.51 |
0.9 | 3078 | 3084 | 3081 | 3020.4 | 1.97 |
1.0 | 3578 | 3556 | 3567 | 3356 | 5.92 |
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