CN104004018B - 具有含氮脂环式骨架的单链核酸分子 - Google Patents

具有含氮脂环式骨架的单链核酸分子 Download PDF

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CN104004018B
CN104004018B CN201410217071.7A CN201410217071A CN104004018B CN 104004018 B CN104004018 B CN 104004018B CN 201410217071 A CN201410217071 A CN 201410217071A CN 104004018 B CN104004018 B CN 104004018B
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expression
rna
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CN104004018A (zh
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大木忠明
林宏刚
白水久男
滨崎智洋
伊藤彰浩
铃木宽
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Bonac Corp
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D207/04—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D207/08—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon radicals, substituted by hetero atoms, attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
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    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance
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    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pyrrole Compounds (AREA)
  • Hydrogenated Pyridines (AREA)
  • Medicinal Preparation (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201410217071.7A 2010-08-03 2011-07-28 具有含氮脂环式骨架的单链核酸分子 Expired - Fee Related CN104004018B (zh)

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Application Number Priority Date Filing Date Title
JP2010-174915 2010-08-03
JP2010174915 2010-08-03
JP2010-230806 2010-10-13
JP2010230806 2010-10-13
JP2010-269823 2010-12-02
JP2010269823 2010-12-02
JP2011-152381 2011-07-08
JP2011152381 2011-07-08
CN201180037592.9A CN103052711B (zh) 2010-08-03 2011-07-28 具有含氮脂环式骨架的单链核酸分子

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CN104004018A CN104004018A (zh) 2014-08-27
CN104004018B true CN104004018B (zh) 2017-01-04

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CN201410217512.3A Expired - Fee Related CN104059911B (zh) 2010-08-03 2011-07-28 具有含氮脂环式骨架的单链核酸分子
CN201180037592.9A Expired - Fee Related CN103052711B (zh) 2010-08-03 2011-07-28 具有含氮脂环式骨架的单链核酸分子

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CN201180037592.9A Expired - Fee Related CN103052711B (zh) 2010-08-03 2011-07-28 具有含氮脂环式骨架的单链核酸分子

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DK (1) DK2674494T3 (enExample)
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PT (1) PT2674494E (enExample)
TW (2) TWI572716B (enExample)
WO (1) WO2012017919A1 (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691782B2 (en) 2010-08-03 2014-04-08 Bonac Corporation Single-stranded nucleic acid molecule having nitrogen-containing alicyclic skeleton
WO2013077446A1 (ja) * 2011-11-26 2013-05-30 株式会社ボナック 遺伝子発現制御のための一本鎖核酸分子
US9404108B2 (en) 2012-03-04 2016-08-02 Bonac Corporation Micro-RMA inhibitor
WO2013133393A1 (ja) * 2012-03-07 2013-09-12 学校法人東京医科大学 Ｖｅｇｆ遺伝子の発現抑制用一本鎖核酸分子
US20150105442A1 (en) * 2012-03-29 2015-04-16 Aqua Therapeutics Co., Ltd. Nucleic Acid Molecule Capable of Inhibiting Expression of Periostin Gene, method for Inhibiting Expression of Periostin Gene, and Use of Said Nucleic Acid Molecule
US9663784B2 (en) 2012-05-26 2017-05-30 Bonac Corporation Single-stranded nucleic acid molecule for regulating expression of gene having delivering function
US20160304866A1 (en) * 2013-09-27 2016-10-20 Aqua Therapeutics Co., Ltd. Drug for Disease Caused by Expression of Periostin Except Eye Disease, and Use Thereof
WO2015093495A1 (ja) * 2013-12-16 2015-06-25 株式会社ボナック ＴＧＦ－β１遺伝子発現制御のための一本鎖核酸分子
CN105899663B (zh) 2013-12-26 2019-07-26 学校法人东京医科大学 用于基因表达控制的人工模拟miRNA及其用途
CN106068324B (zh) * 2013-12-27 2020-12-29 株式会社博纳克 控制基因表达的人工匹配型miRNA及其用途
WO2015099188A1 (ja) * 2013-12-27 2015-07-02 株式会社ボナック 遺伝子発現制御のための人工マッチ型ｍｉＲＮＡおよびその用途
HK1244035A1 (en) * 2014-12-15 2018-07-27 Bonac Corporation Single-stranded nucleic acid molecule for inhibiting tgf- beta 1 expression
BR112017013664A2 (pt) * 2014-12-27 2018-03-13 Bonac Corporation mirna de tipo natural, composição, métodos para inibir expressão de um gene alvo e para tratar uma doença, e, ácido nucleico de fita simples.
HK1244031A1 (en) 2015-03-27 2018-07-27 Bonac Corporation Single-chain nucleic acid molecule having delivery function and gene expression control ability
JPWO2016167366A1 (ja) * 2015-04-17 2018-04-05 国立大学法人 東京大学 眼疾患治療剤
PL3369816T3 (pl) 2015-10-30 2024-07-22 Toray Industries, Inc. KOMPOZYCJA STABILNIE ZAWIERAJĄCA CZĄSTECZKĘ JEDNONICIOWEGO KWASU NUKLEINOWEGO, KTÓRA HAMUJE EKSPRESJĘ GENU TGF-β1
JP6634095B2 (ja) 2015-12-29 2020-01-22 国立大学法人北海道大学 プロレニン遺伝子またはプロレニン受容体遺伝子の発現を抑制する一本鎖核酸分子およびその用途
EP3409779A4 (en) * 2016-01-26 2019-07-03 Nissan Chemical Corporation Single-stranded oligonucleotide
US11518994B2 (en) 2016-01-30 2022-12-06 Bonac Corporation Artificial single guide RNA and use thereof
CN109071579B (zh) * 2016-04-26 2021-05-14 住友化学株式会社 单链核酸分子用单体的制造方法
MX2019009305A (es) 2017-02-06 2019-09-19 Nissan Chemical Corp Oligonucleotido de cadena simple.
JPWO2018182008A1 (ja) 2017-03-31 2020-02-06 株式会社ボナック 遺伝子発現制御機能を有する環状型核酸分子
JPWO2018199338A1 (ja) * 2017-04-27 2020-03-12 国立大学法人広島大学 Ｂ型肝炎治療用核酸分子
SG11201913541QA (en) * 2017-07-28 2020-02-27 Kyorin Pharmaceutical Co Ltd Therapeutic agent for fibrosis
US20210188895A1 (en) 2017-10-13 2021-06-24 Bonac Corporation Single-stranded nucleic acid molecule, and production method therefor
TWI830718B (zh) * 2018-02-09 2024-02-01 日商住友化學股份有限公司 核酸分子之製造方法
EP3770256A4 (en) 2018-03-20 2021-12-22 Tokyo Institute of Technology OLIGONUCLEOTIDE ANTISENS WITH REDUCED TOXICITY
US11920131B2 (en) 2018-03-30 2024-03-05 Toray Industries, Inc. Method of producing hairpin single-stranded RNA molecule
US20210115485A1 (en) 2018-03-30 2021-04-22 Sumitomo Chemical Company, Limited Method for producing single-strand rna
JP7405086B2 (ja) * 2018-10-02 2023-12-26 東レ株式会社 ヘアピン型一本鎖ｒｎａ分子の製造方法
JP7241098B2 (ja) 2019-01-25 2023-03-16 杏林製薬株式会社 線維症治療剤
WO2020202952A1 (ja) 2019-03-29 2020-10-08 住友化学株式会社 無機多孔質担体、及びこれを用いた核酸の製造方法
EP3950695A4 (en) 2019-03-29 2023-01-25 Sumitomo Chemical Company, Limited PROCEDURE FOR PRODUCTION OF RNA
WO2020202953A1 (ja) 2019-03-29 2020-10-08 住友化学株式会社 無機多孔質担体、及びこれを用いた核酸の製造方法
WO2020202951A1 (ja) 2019-03-29 2020-10-08 住友化学株式会社 無機多孔質担体、及びこれを用いた核酸の製造方法
US12371451B2 (en) 2019-03-29 2025-07-29 Sumitomo Chemical Company, Limited Inorganic porous carrier and method for producing nucleic acids using same
JP7640461B2 (ja) 2019-10-11 2025-03-05 住友化学株式会社 核酸オリゴマーの製造方法
CN115003682A (zh) 2020-01-29 2022-09-02 住友化学株式会社 核酸寡聚物的制造方法
US12391716B2 (en) 2020-01-29 2025-08-19 Sumitomo Chemical Company, Limited Process of preparing nucleic acid oligomer
WO2021193954A1 (ja) 2020-03-27 2021-09-30 住友化学株式会社 核酸オリゴマーの製造方法
US20230256425A1 (en) 2020-05-13 2023-08-17 Sumitomo Chemical Company, Limited Inorganic porous substrate, inorganic porous support, and nucleic acid production method
EP4180442A4 (en) 2020-07-09 2025-05-14 Sumitomo Chemical Company, Limited METHOD FOR PRODUCING A NUCLEIC ACID OLIGOMER
US20240051986A1 (en) * 2020-09-24 2024-02-15 Sumitomo Chemical Company, Limited Method for producing nucleic acid oligomer
EP4410770A1 (en) 2021-09-28 2024-08-07 Sumitomo Chemical Company, Limited Production method for purified dichloroacetic acid
EP4488282A4 (en) 2022-03-23 2025-11-19 Sumitomo Chemical Co PROCESS FOR THE PRODUCTION OF NUCLEIC ACID OLIGOMERS

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
GB9621367D0 (en) * 1996-10-14 1996-12-04 Isis Innovation Chiral peptide nucleic acids
US6509323B1 (en) 1998-07-01 2003-01-21 California Institute Of Technology Linear cyclodextrin copolymers
WO2005019453A2 (en) * 2001-05-18 2005-03-03 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
US6962906B2 (en) * 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
TWI321054B (en) 2000-12-19 2010-03-01 California Inst Of Techn Compositions containing inclusion complexes
US20060111312A1 (en) * 2002-02-22 2006-05-25 The John Hopkins University Antigene locks and therapeutic uses thereof
US20040058886A1 (en) 2002-08-08 2004-03-25 Dharmacon, Inc. Short interfering RNAs having a hairpin structure containing a non-nucleotide loop
NL1022811C2 (nl) * 2003-02-28 2004-08-31 Fountain Patents B V Systeem en werkwijze voor opslag en transport van zeecontainers.
EP1608735A4 (en) * 2003-04-03 2008-11-05 Alnylam Pharmaceuticals RNAI CONJUGATES
JPWO2005030960A1 (ja) * 2003-09-30 2006-12-07 アンジェスＭｇ株式会社 ステイプル型オリゴヌクレオチドおよびそれからなる医薬
WO2006088490A2 (en) * 2004-06-30 2006-08-24 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising a non-phosphate backbone linkage
WO2006074108A2 (en) * 2004-12-30 2006-07-13 Hauser Todd M Compositions and methods for modulating gene expression using self-protected oligonucleotides
PL2129680T3 (pl) * 2007-03-21 2015-10-30 Brookhaven Science Ass Llc Łączone kompozycje cząsteczek antysensownych o strukturze spinki do włosów oraz sposoby modulacji ekspresji
CN101889087B (zh) * 2007-11-29 2013-04-03 苏州瑞博生物技术有限公司 一种干扰靶基因表达的复合分子及其制备方法
CA2930393C (en) * 2007-12-04 2022-11-29 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
WO2009076321A2 (en) * 2007-12-07 2009-06-18 Halo-Bio Rnai Therapeutics Inc. Compositions and methods for modulating gene expression using asymmetrically-active precursor polynucleotides
JP2010174915A (ja) 2009-01-27 2010-08-12 Toyota Motor Corp 高圧ガスタンク
JP5449815B2 (ja) 2009-03-26 2014-03-19 住友化学株式会社 偏光板の製造方法
JP2010269823A (ja) 2009-05-21 2010-12-02 British American Tobacco Japan Kk 喫煙品用容器
JP2011152381A (ja) 2010-01-25 2011-08-11 Ucom:Kk 箸置き

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Antimycobacterial Activity of Ionic Fullerene Derivatives;Susanna Bosi, et al.;《Bioorg. Med. Chem. Lett.》;20001231;第10卷;1043-1045 *
Comparison of Solution-Phase and Solid-Phase Syntheses of a Restrained Proline-Containing Analogue of the Nodularin Macrocycle;Kerri L, Webster, et al.;《Tetrahedron Letters》;19971231;第38卷(第32期);5713-5716 *
Pyrrolidine Nucleic Acids: DNA/PNA Oligomers with 2-Hydroxy/Aminomethyl-4-(thymin-1-yl) pyrrolidine-N-acetic acid;Vaijayanti Kumar，et al.;《Org. Lett.》;20010411;第3卷(第9期);1269-1272 *

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TW201615835A (zh) 2016-05-01
DK2674494T3 (en) 2015-02-23
KR101894701B1 (ko) 2018-09-04
EP2639307A8 (en) 2014-04-30
JP2013138681A (ja) 2013-07-18
WO2012017919A1 (ja) 2012-02-09
JP4965745B2 (ja) 2012-07-04
ES2528285T3 (es) 2015-02-06
JP2013034470A (ja) 2013-02-21
EP2628801B1 (en) 2015-01-07
EP2639307A3 (en) 2014-02-05
EP2436767A4 (en) 2012-05-09
ES2533129T3 (es) 2015-04-07
EP2639307A2 (en) 2013-09-18
EP2674494B1 (en) 2014-12-17
TWI572716B (zh) 2017-03-01
JPWO2012017919A1 (ja) 2013-10-03
KR101894702B1 (ko) 2018-09-04
HK1200460A1 (en) 2015-08-07
ES2527660T3 (es) 2015-01-28
PT2674494E (pt) 2015-02-27
TWI515294B (zh) 2016-01-01
CN104059911A (zh) 2014-09-24
CN103052711A (zh) 2013-04-17
JP5261677B2 (ja) 2013-08-14
KR20180040728A (ko) 2018-04-20
KR101849801B1 (ko) 2018-04-17
CN103052711B (zh) 2016-08-03
TW201219570A (en) 2012-05-16
EP2639307B1 (en) 2015-03-04
JP5555346B2 (ja) 2014-07-23
HK1189910A1 (en) 2014-06-20
EP2436767A1 (en) 2012-04-04
CN104059911B (zh) 2017-01-04
CN104004018A (zh) 2014-08-27
EP2436767B1 (en) 2013-11-20
EP2628801A1 (en) 2013-08-21
KR20130095738A (ko) 2013-08-28
EP2674494A1 (en) 2013-12-18
KR20180040727A (ko) 2018-04-20
ES2443346T3 (es) 2014-02-19

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