CN103858897B - A kind of method that autumn is sent out element and impels shallow dormancy tree peony to bloom National Day - Google Patents
A kind of method that autumn is sent out element and impels shallow dormancy tree peony to bloom National Day Download PDFInfo
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Abstract
本发明提供了一种秋发素及促使浅休眠牡丹国庆开花的方法。所述秋发素包含赤霉素、生长素和玉米素。所述方法包括如下步骤:1)选取秋发牡丹品种的植株;2)对所述植株进行脱叶处理;3)使用秋发素对经过脱叶处理的植株的芽体进行涂抹处理,所述秋发素包含赤霉素、生长素和玉米素。本发明采用了浅休眠品种牡丹,综合利用脱叶与激素配合解除休眠,可以在节省能源的情况下实现牡丹国庆期间的开放,并在适宜时间上盆,防止上盆后植株花、叶萎蔫。与露地牡丹相比,盆栽牡丹具有更广泛的应用。The invention provides an autumn hair essence and a method for promoting the flowering of the shallow dormant peony on National Day. The autumn hairin contains gibberellin, auxin and zeatin. The method comprises the following steps: 1) selecting a plant of the autumn-haired tree peony variety; 2) performing defoliation treatment on the plant; 3) using autumn hair element to smear the buds of the defoliation-treated plant, and the Qiufasu contains gibberellin, auxin and zeatin. The invention adopts the shallow dormancy variety tree peony, comprehensively utilizes defoliation and hormones to release dormancy, can realize the opening of the peony during the National Day under the condition of saving energy, and pots it at a suitable time to prevent the flowers and leaves of the plant from wilting after potting. Compared with open field peonies, potted peonies have a wider range of applications.
Description
本申请是发明名称为“一种盆栽牡丹国庆开花的促成栽培方法”、申请号为“201210077211.6”、申请日为“2012年03月22日”的中国发明专利申请的分案申请。This application is a divisional application of a Chinese invention patent application titled "A Method for Promoting Blooming of Potted Peonies on National Day", the application number is "201210077211.6", and the application date is "March 22, 2012".
技术领域technical field
本发明涉及植物的栽培方法,更具体地说,本发明涉及牡丹的栽培方法。The present invention relates to the cultivation method of plant, more specifically, the present invention relates to the cultivation method of peony.
背景技术Background technique
牡丹,学名为PaeoniasuffruticosaAndr,属于芍药科(Paeoniaceae)芍药属(Paeonia)植物,原产于中国,在我国已有1600多年的历史。汉中是中国最早人工栽培牡丹的地方,它的花和叶的形态同芍药很相像,所以古时又称牡丹为木芍药。Peony, scientific name Paeoniasuffruticosa Andr, belongs to Paeoniaceae (Paeoniaceae) Paeonia (Paeonia) plant, originates in China, has a history of more than 1600 years in my country. Hanzhong is the first place in China to artificially cultivate peony. Its flowers and leaves are very similar to peony, so peony was also called wood peony in ancient times.
牡丹以其体态优美、花朵硕大、花色秀丽、色泽丰富等非凡特色,被人们誉为“花中之王”和“富贵之花”,更是美好、幸福、吉祥和富贵的象征,深受我国各族人民和海外人士的喜爱。牡丹观赏部位主要是花朵,其花朵雍容华贵、富丽堂皇著称。牡丹可在公园和风景区建立专类园;在古典园林和居民院落中筑花台种植;在园林绿地中自然式孤植、丛植或片植。也适用于布置花境、花坛或花带,或者盆栽观赏,在应用上非常灵活。根皮可入药,花瓣可酿酒,可以说,牡丹各个器官均具有重要的经济价值。Peony is known as the "king of flowers" and "the flower of wealth" for its extraordinary characteristics such as beautiful body, huge flowers, beautiful colors, and rich colors. It is also a symbol of beauty, happiness, auspiciousness, and wealth. Loved by people of all nationalities and overseas. The ornamental parts of peonies are mainly flowers, which are famous for their elegance and magnificence. Peonies can be planted in special gardens in parks and scenic spots; in classical gardens and residential courtyards, planted on flower beds; in gardens and green spaces, they can be planted naturally in isolation, clusters or in patches. It is also suitable for arranging flower borders, flower beds or flower belts, or potted plants, and is very flexible in application. The root bark can be used as medicine, and the petals can be used to make wine. It can be said that each organ of peony has important economic value.
一般来讲,起源于温带地区的园艺植物,在其年生长发育周期中,一般于春季开花,夏秋进行花芽分化,秋末落叶,入冬休眠,而在一些特殊的情况下,某些种类也可以在未经休眠或提前解除休眠的情况下,于当年二次生长,二次开花,这是一种物候期重演现象。某些牡丹品种就具有物候期在一年中在一定条件下的重演性,即牡丹混合芽在当年花芽分化完成或部分完成(进入雌雄蕊瓣化期)之后,在适宜的内外条件下,不经过冬季的休眠过程,于当年秋季萌动生长、显蕾开花。我们将这种秋季容易萌动生长的牡丹品种简称为“秋发牡丹”(王莲英,1998)。Generally speaking, horticultural plants originating from temperate regions generally flower in spring, undergo flower bud differentiation in summer and autumn, fall leaves in late autumn, and go dormant in winter during their annual growth and development cycle. In the case of no dormancy or early release of dormancy, the second growth and second flowering in the same year is a phenomenon of phenological recurrence. Some peony varieties have the recurring phenological period under certain conditions in a year, that is, after the flower bud differentiation of peony is completed or partially completed (entering the stage of pistil and stamen petalization) in the current year, under suitable internal and external conditions, no After the dormancy process in winter, it germinates, grows, buds and blooms in the autumn of that year. We abbreviate this peony variety that is easy to germinate and grow in autumn as "Qiu Fa Peony" (Wang Lianying, 1998).
秋发牡丹品种是众多牡丹品种中较为特殊的一类,它们能在适宜的自然条件下,于秋季二次生长,二次开花,且经过简单的人工诱导已经可以于秋季露地二次开花,不仅满足了人们两季观赏牡丹的愿望而且二次开花栽培成本较低,具有广阔的发展前景,为牡丹国庆节催花的产业化生产奠定坚实的基础。Autumn peony varieties are a special kind among many peony varieties. They can grow and bloom for the second time in autumn under suitable natural conditions, and they can bloom for the second time in the open field in autumn after simple artificial induction. It satisfies people's desire to appreciate peonies in two seasons, and the cost of secondary flowering cultivation is low, has broad development prospects, and lays a solid foundation for the industrial production of peonies for National Day.
如上所述,牡丹的自然花期相对较短,很难满足各种节日用花的需求。例如,如果不通过花期调控,在国庆期间,市场上是看不到开花的牡丹的。通过适时脱叶,再加上适当的激素处理,可以使某些品种达到在国庆节前后开花。As mentioned above, the natural flowering period of peonies is relatively short, and it is difficult to meet the needs of various festival flowers. For example, if the flowering period is not controlled, there will be no blooming peonies in the market during the National Day. Through timely defoliation, coupled with appropriate hormone treatment, some varieties can achieve flowering around the National Day.
本发明人发现,选择具有浅休眠特性。对低温需求量低的特殊品种,在恰当的时候人工脱叶,结合适宜的激素浓度处理,可使牡丹达到国庆节开花的目的。The inventors found that selection has a shallow dormancy property. For special species with low demand for low temperature, manual defoliation at the right time, combined with appropriate hormone concentration treatment, can make the peony achieve the purpose of flowering on National Day.
发明内容Contents of the invention
为了解决上述的一个或多个问题,本发明提供了如下实施方案。In order to solve one or more of the above-mentioned problems, the present invention provides the following embodiments.
1、一种盆栽牡丹国庆开花的促成栽培方法,所述方法包括如下步骤:1. A method for promoting cultivation of potted peony blooming on National Day, said method comprising the steps of:
1)选取秋发牡丹品种的植株;1) select the plant of the autumn peony variety;
2)对所述植株进行脱叶处理;2) Defoliating the plant;
3)使用秋发素对经过脱叶处理的植株的芽体进行涂抹处理,其中,所述秋发素包含600mg/L至900mg/L的赤霉素;3) smearing the buds of the defoliation-treated plants with qiufa, wherein the qiufa contains 600mg/L to 900mg/L of gibberellins;
4)上盆。4) Upper basin.
2、如技术方案1所述的方法,其中,所述秋发牡丹品种为‘藏枝红’(paeoniasuffruticosa‘cangzhihong’)或‘西瓜瓤’(paeoniasuffruticosa‘xiguarang’)。2. The method according to technical solution 1, wherein the species of peony of autumn hair is 'paeoniasuffruticosa 'cangzhihong' or 'watermelon flesh' (paeoniasuffruticosa 'xiguarang').
3、如技术方案1或2所述的方法,其中,所述植株为4至5年生的壮龄植株,优选每植株具有6至8个主枝,进一步优选每植株至少具有6至10个以上饱满顶芽。3. The method according to technical solution 1 or 2, wherein the plant is a 4-5 year old plant, preferably with 6 to 8 main branches per plant, more preferably at least 6 to 10 main branches per plant Full buds.
4、如技术方案1至3中任一项所述的方法,其中,所述脱叶处理为全脱叶处理,优选通过人工脱叶方式进行。4. The method according to any one of technical solutions 1 to 3, wherein the defoliation treatment is complete defoliation treatment, preferably by manual defoliation.
5、如技术方案1至4中任一项所述的方法,其中,所述秋发素进一步包含生长素和玉米素,优选所述赤霉素、生长素和玉米素的重量百分比为8∶1∶1。5. The method according to any one of technical schemes 1 to 4, wherein the qiufalin further comprises auxin and zeatin, preferably the weight percentage of gibberellin, auxin and zeatin is 8: 1:1.
6、如技术方案1至5中任一项所述的方法,其中,所述秋发素采用浓度逐渐提高法进行涂抹处理。6. The method according to any one of technical solutions 1 to 5, wherein the qiufa essence is applied by gradually increasing the concentration.
7、如技术方案至6所述的方法,其中,所述涂抹处理采用如下方式进行:每隔3天涂抹芽体一次,连续涂抹3次,其中3次涂抹处理中的秋发素中的赤霉素浓度依次分别为800mg/L,1200mg/L,1600mg/L;优选对顶芽进行涂抹处理,同时抹掉植株上所有萌发的其它芽体以及植株基部的萌蘖芽。7. The method as described in technical scheme to 6, wherein, the smearing process is carried out in the following manner: smearing the bud body once every 3 days, and smearing it continuously for 3 times, wherein the red in the autumn hair element in the 3 times of smearing process is The concentrations of mycin are 800mg/L, 1200mg/L, and 1600mg/L respectively; it is preferable to smear the terminal buds, and at the same time wipe off all other buds that germinate on the plant and the tiller buds at the base of the plant.
8、如技术方案1至7中任一项所述的方法,其中,所述上盆采用带土坨的方式进行,优选在花蕾处于圆蕾期至透色期进行上盆,更优选在上盆后浇灌一次透水。8. The method according to any one of technical solutions 1 to 7, wherein the potting is carried out with lumps of soil, preferably when the flower buds are in the round bud stage to the transparent stage, more preferably in the upper potting stage. After potting, water once permeable.
9、如技术方案8所述的方法,其中,在上盆时,植株根系的根长保留为35cm至40cm。9. The method according to technical solution 8, wherein, when potting, the root length of the plant root system remains at 35cm to 40cm.
10、如技术方案1至9中任一项所述的方法,其中,所述方法在所述脱叶处理之后并且在所述涂抹处理之前还包括对经脱叶处理的植株进行10至15天的控水处理。10. The method according to any one of technical schemes 1 to 9, wherein, after the defoliation treatment and before the smear treatment, the method also includes carrying out the defoliation treatment for 10 to 15 days. water control treatment.
11、如技术方案1至10中任一项所述的方法,其中,所述方法在所述控水处理结束后至上盆前还包括浇透水处理。11. The method according to any one of technical solutions 1 to 10, wherein, after the water control treatment is completed and before the basin is placed, the method further includes watering through treatment.
12、如技术方案11所述的方法,其中,每两周浇一次透水,优选同时将环境的空气相对湿度保持70%-80%。12. The method according to technical solution 11, wherein watering is carried out every two weeks, preferably while maintaining the relative air humidity of the environment at 70%-80%.
13、如技术方案1至12中任一项所述的方法,其中,所述方法在上盆之后还包括缓苗处理步骤。13. The method according to any one of technical solutions 1 to 12, wherein the method further includes a seedling slowing treatment step after potting.
14、如技术方案13所述的方法,其中,所述缓苗通过如下方式进行:将上盆后的植株于阴凉处放置3-5天,优选昼夜温度不高于15℃,进一步优选将环境相对湿度保持在80%-90%。14. The method according to technical solution 13, wherein the slowing down of seedlings is carried out in the following manner: place the potted plants in a cool place for 3-5 days, preferably the day and night temperature is not higher than 15°C, and more preferably the environment The relative humidity is maintained at 80%-90%.
15、如技术方案1至14中任一项所述的方法,其中,所述方法在缓苗处理之后还包括炼苗处理步骤。15. The method according to any one of technical solutions 1 to 14, wherein the method further includes a seedling hardening treatment step after the seedling slowing treatment.
16、如技术方案15所述的方法,其中,炼苗时间为3至5天,以使盆栽牡丹在国庆节期间开放。16. The method according to technical solution 15, wherein the seedling hardening time is 3 to 5 days, so that the potted peonies will open during the National Day.
由于本发明利用了浅休眠牡丹品种秋季二次开花的特性,利用自然环境加人工处理解除休眠,可以在节省能源的情况下实现牡丹国庆期间的开放。本发明方法可以解决一个或多个如下问题:花朵畸形(激素处理不当容易造成畸形,适当的方法涂抹激素可以有效防止花朵畸形;花叶比例失调;根系生长不良,即在一些优选的实施方式中,通过选择合适的时期上盆,可以有效促进根系生长;能耗高,生产成本大,本发明方法不需要放入冷库来解除芽体的休眠。Because the present invention utilizes the characteristics of secondary flowering in autumn of shallow dormant peony varieties, the natural environment and artificial treatment are used to release dormancy, and the opening of the peony during the National Day can be realized under the condition of saving energy. The method of the present invention can solve one or more of the following problems: deformed flowers (improper treatment of hormones can easily cause deformed flowers, and applying hormones in an appropriate way can effectively prevent deformed flowers; the proportion of flowers and leaves is out of balance; root growth is poor, that is, in some preferred embodiments , by selecting a suitable period for potting, root growth can be effectively promoted; energy consumption is high, and production cost is high, and the method of the present invention does not need to be placed in a cold storage to release the dormancy of the buds.
具体实施方式detailed description
如上所述,本发明提供了一种国庆开花盆栽牡丹促成栽培的方法,即待用于国庆开花的盆栽牡丹的催花方法。As mentioned above, the present invention provides a method for promoting the cultivation of potted peonies for National Day flowering, that is, a method for promoting flowering of potted peonies to be used for National Day flowering.
在本发明的第一方面,提供了一种盆栽牡丹国庆开花的促成栽培方法,所述方法包括如下步骤:1)选取秋发牡丹品种的植株;2)对所述植株进行脱叶处理;3)使用秋发素对经过脱叶处理的植株的芽体进行涂抹处理,其中,所述秋发素包含600mg/L至900mg/L的赤霉素;In the first aspect of the present invention, there is provided a cultivation method for promoting flowering of potted peony on National Day, said method comprising the following steps: 1) selecting a plant of a species of peony with autumn hair; 2) carrying out defoliation treatment on said plant; 3 ) smearing the buds of the defoliation-treated plants with autumn hairin, wherein the autumn hairin contains 600mg/L to 900mg/L gibberellins;
4)上盆。4) Upper basin.
一般来讲,牡丹起源于温带地区的园艺植物,在其年生长发育周期中,一般于夏季开花,秋季进行花芽分化,秋末落叶,入冬休眠,而在一定条件下,某些牡丹品种也可以在未经休眠或提前解除休眠的情况下,于当年二次生长,二次开花,这是一种物候期重演现象,具有中重演现象的牡丹的混合芽在当年花芽分化完成或部分完成(即进入雌雄蕊瓣化期)之后,在适宜的内外条件下,不经过冬季的休眠过程,于当年秋季萌动生长、显蕾开花。我们将这种具有秋季萌动生长倾向或现象的牡丹品种简称为“秋发牡丹”(王莲英,1998)。Generally speaking, peonies originate from horticultural plants in temperate regions. During their annual growth and development cycle, they usually bloom in summer, undergo flower bud differentiation in autumn, shed leaves in late autumn, and dormant in winter. Under certain conditions, some peony varieties can also In the case of no dormancy or early release of dormancy, the secondary growth and secondary flowering in the same year are a kind of phenological recurrence phenomenon. The mixed buds of peonies with medium recurrence phenomenon complete or partially complete the flower bud differentiation in the current year (ie After entering the pistil petalization period), under suitable internal and external conditions, without going through the dormancy process in winter, it will germinate, grow, bud and bloom in the autumn of that year. We abbreviate this peony variety with the tendency or phenomenon of sprouting growth in autumn as "Qiufa Peony" (Wang Lianying, 1998).
本发明的具体的牡丹品种没有特殊限制,只要是秋发牡丹即可。但是在一些优选的实施方式中,所述秋发牡丹品种为‘藏枝红’(paeoniasuffruticosa‘cangzhihong’)或‘西瓜瓤’(paeoniasuffruticosa‘xiguarang’)。The specific peony varieties of the present invention are not particularly limited, as long as they are autumn peony. But in some preferred embodiments, the qiufa peony variety is 'paeoniasuffruticosa 'cangzhihong' or 'watermelon pulp' (paeoniasuffruticosa 'xiguarang').
本发明对秋发牡丹的株龄没有特别限制,但是优选为4至5年生的壮龄植株。如果株龄过小,则枝条营养不够,难以成花;如果株龄过大,植株上水困难,难以成花。The present invention has no special limitation on the plant age of the autumn peony, but it is preferably a 4 to 5-year-old prime-age plant. If the plant age is too young, the branches will not have enough nutrition and it will be difficult to form flowers; if the plant age is too large, it will be difficult for the plants to get water and it will be difficult to form flowers.
在一些实施方式中,进一步优选每植株具有6至8个主枝;如果主枝过多,则不易于盆栽管理,且争夺养分,从而影响成花质量;如果主枝过少,则影响盆栽牡丹整体美观。In some embodiments, it is further preferred that each plant has 6 to 8 main branches; if there are too many main branches, it is not easy to manage potted plants and compete for nutrients, thereby affecting the quality of flowers; if there are too few main branches, it will affect the potted peony The overall beauty.
另外,在一些优选实施方式中,优选每植株至少具有6至10个以上饱满顶芽。如果顶芽过多,则养分分散,影响成花质量;如果顶芽过少,则成花较少影响整体美观。In addition, in some preferred embodiments, each plant preferably has at least 6 to 10 full terminal buds. If there are too many terminal buds, the nutrients will be dispersed, which will affect the quality of the flowers; if there are too few terminal buds, the fewer flowers will affect the overall appearance.
为了使秋发牡丹能够在当年二次开花,一般情况下,特别是对于盆栽牡丹而言,需要人工打破混合芽的休眠,脱叶处理被认为是打破秋发牡丹休眠的一种手段。为了打破休眠,可以对秋发牡丹进行进行单枝脱叶、半株脱叶或者全株脱叶。但是在本发明的方法中,优选采用全脱叶处理。In order to make the peonies of autumn hair bloom again in the same year, in general, especially for potted peonies, it is necessary to manually break the dormancy of the mixed buds. Defoliation treatment is considered as a means to break the dormancy of peonies of autumn hair. In order to break dormancy, you can defoliate single branch, half plant or whole plant of Qiufa peony. However, in the method of the present invention, it is preferred to use full defoliation.
本发明对脱叶方式没有特别限制,例如可以采用物理脱叶(不借助于化学试剂所进行的脱叶方式)或化学脱叶(例如采用乙烯或脱落酸或其他脱叶剂进行脱叶)。在本发明的方法中,优选采用物理脱叶的方式。在一些实施方式中,优选采用人工物理脱叶。The method of defoliation is not particularly limited in the present invention, for example, physical defoliation (defoliation without the aid of chemical reagents) or chemical defoliation (such as defoliation using ethylene or abscisic acid or other defoliants) can be used. In the method of the present invention, physical defoliation is preferably used. In some embodiments, manual physical defoliation is preferred.
本发明对人工脱叶时间没有严格要求,一般是在国庆节前40~50天为宜,脱叶阻止秋季叶片中大量的ABA转运至混合芽,使芽体中ABA含量保持较低水平,从而促进了混合芽的萌发。从混合芽的萌发到开花中间可划分为脱叶前期、萌动期、显蕾期、跳蕾期、小风铃期、撒飘期、圆蕾期、初花期、盛花期等9个阶段,历时约45天,脱叶时间可能因品种及植株营养状况的不同而有所不同。The present invention does not have strict requirements on the artificial defoliation time, generally 40-50 days before the National Day, defoliation prevents a large amount of ABA in autumn leaves from being transported to mixed buds, and keeps the ABA content in buds at a low level, thereby Promoted the germination of mixed buds. From the germination of the mixed buds to the flowering, it can be divided into 9 stages: the early stage of leaf removal, the germination stage, the bud display stage, the bud jumping stage, the small wind bell stage, the floating stage, the round bud stage, the initial flowering stage, and the full flowering stage. 45 days, the defoliation time may vary according to the variety and the nutritional status of the plant.
在本发明中,采用秋发素对芽体进行涂抹处理。在本发明中,术语“秋发素”是指能够促进牡丹秋季萌发以便二次开花的物质。在本发明的一些实施方式中,所述秋发素是含有规定浓度的赤霉素的溶液,优选为水溶液。赤霉素是广泛存在的一类植物激素,其化学结构属于二萜类酸,由四环骨架衍生而得。一般认为可以刺激叶和芽的生长,但是不同的植物种类或者同科植物不同品种对赤霉素的响应差异很大,并且具有明显不同的浓度耐受性。目前,已知的赤霉素类物质不止一种。在本发明中,优选采用的赤霉素为GA3。赤霉素的浓度处在600mg/L至900mg/L为佳,例如为600mg/L、650mg/L、700mg/L、750mg/L、800mg/L、850mg/L或900mg/L。如果赤霉素的浓度过大,则刺激叶片过早发育,造成“叶吃花”的现象,即造成叶多花少的现象;如果赤霉素的浓度过小,则难以达到预期效果。In the present invention, the sprouts are smeared with Qiufasu. In the present invention, the term "autumn" refers to a substance that can promote autumn germination of peonies for secondary flowering. In some embodiments of the present invention, the qiufalin is a solution containing gibberellin at a specified concentration, preferably an aqueous solution. Gibberellin is a widely existing class of plant hormones, and its chemical structure belongs to diterpenoid acids, derived from a tetracyclic skeleton. It is generally believed that it can stimulate the growth of leaves and buds, but different plant species or different varieties of plants of the same family have very different responses to gibberellin, and have significantly different concentration tolerances. At present, more than one gibberellins are known. In the present invention, the gibberellin preferably used is GA 3 . The concentration of gibberellin is preferably 600mg/L to 900mg/L, such as 600mg/L, 650mg/L, 700mg/L, 750mg/L, 800mg/L, 850mg/L or 900mg/L. If the concentration of gibberellin is too high, it will stimulate the leaves to develop prematurely, resulting in the phenomenon of "leaves eating flowers", that is, more leaves and less flowers; if the concentration of gibberellin is too small, it will be difficult to achieve the desired effect.
在本发明的一些实施方式中,优选所述秋发素进一步包含生长素和玉米素。本发明人在试验过程中意外地发现,向秋发素中添加生长素和玉米素,能够达到明显较好的结果,而单独添加玉米素和玉米素中的一种,基本没有效果。在一些实施方式中,优选所述赤霉素、生长素和玉米素的重量百分比为8∶1∶1。In some embodiments of the present invention, it is preferred that the autumn hairin further comprises auxin and zeatin. During the experiment, the inventor unexpectedly found that adding auxin and zeatin to qiufasu can achieve significantly better results, while adding either zeatin or zeatin alone has basically no effect. In some embodiments, the weight percentage of gibberellin, auxin and zeatin is preferably 8:1:1.
在本发明所述的秋发素中,赤霉素例如GA3常被用作农用植物的生长调节剂。GA3的生理作用为促进作用和打破休眠作用,在生产上的主要作用是促进营养生长和打破休眠。生长素对生长的促进作用主要是促进细胞的生长,特别是细胞的伸长,玉米素和生长素配合使用有助于促进发芽。在一些优选的实施方式中,每10m1秋发素中加入10微升吐温试剂(北京化学试剂公司)。涂抹时,例如可以采用笔尖较软的毛笔蘸取少量的秋发素,每次蘸取的量以没有液体悬滴为宜,涂抹饱满的顶芽或第一位芽。为了避免养分竞争,优选抹去其余混合芽,。Among the autumn hairins of the present invention, gibberellins such as GA 3 are often used as growth regulators for agricultural plants. The physiological role of GA 3 is to promote and break dormancy, and its main role in production is to promote vegetative growth and break dormancy. The growth-promoting effect of auxin is mainly to promote cell growth, especially cell elongation, and the combined use of zeatin and auxin helps to promote germination. In some preferred embodiments, 10 microliters of Tween reagent (Beijing Chemical Reagent Company) was added to every 10 ml of Qiufasu. When smearing, for example, you can use a brush with a soft tip to dip a small amount of autumn hair essence. It is advisable to dip the amount each time without liquid hanging drops, and apply it to the full terminal bud or first bud. To avoid nutrient competition, it is preferable to wipe off the rest of the mixed buds.
本发明对涂抹次数和频率没有特别限制。但是优选的是,所述涂抹处理每隔3天涂抹芽体一次,连续涂抹3次。另外,本发明人在试验中意外地发现,所述秋发素采用浓度逐渐提高法进行涂抹处理可以达到更好的效果。例如,所述涂抹处理每隔3天涂抹芽体一次,连续涂抹3次,其中3次涂抹处理中的秋发素中的赤霉素浓度依次分别为800mg/L,1200mg/L,1600mg/L。另外,在一些优选的实施方式中,对顶芽进行涂抹处理,同时抹掉植株上所有萌发的其它芽体以及植株基部的萌蘖芽。The present invention has no special limitation on the number and frequency of application. However, preferably, the smearing process smears the sprout once every 3 days, and smears continuously for 3 times. In addition, the present inventors unexpectedly found in the experiment that the qiufasu can achieve better effect by adopting the method of gradually increasing the concentration for smearing. For example, the smearing process smears the sprouts once every 3 days, and smears continuously for 3 times, wherein the gibberellin concentrations in the qiufalin in the 3 smearing treatments are respectively 800mg/L, 1200mg/L, and 1600mg/L. . In addition, in some preferred embodiments, the terminal buds are smeared, and all other buds that germinate on the plant and the tillering buds at the base of the plant are wiped off at the same time.
为了使秋发牡丹能够在一年中二次开花,在上盆时,优选采用带土坨的方式进行。为了使秋发牡丹能够在国庆节期间开花,上盆时间优选在花蕾处于圆蕾期至透色期进行上盆,关于所述圆蕾期至透色期的确定,请参照《中国牡丹与芍药》(李嘉珏,1999)。在此将该文献的相关内容引入本文中。其中,所述圆蕾期特征如下:花蕾圆满充实,撒飘平展移于花蕾下部;透色期:萼片裂开,外层花瓣露出,萼片移于花蕾中下部,花蕾变软。In order to make the peony of autumn hair bloom twice in a year, it is preferable to use the method of bringing soil lumps when potting. In order to make the autumn peony bloom during the National Day, the potting time is preferably carried out when the buds are in the round bud stage to the transparent color stage. For the determination of the round bud stage to the transparent color stage, please refer to "Chinese Peony and Paeoniae "(Li Jiajue, 1999). The relevant content of this document is hereby incorporated into this text. Among them, the characteristics of the round bud stage are as follows: the flower bud is full and full, and spreads and moves to the lower part of the flower bud; the transparent stage: the sepals are split, the outer petals are exposed, the sepals are moved to the middle and lower part of the flower bud, and the flower bud becomes soft.
另外,在一些实施方式中,优选在上盆之后,浇灌一次透水。In addition, in some embodiments, it is preferable to water once permeable after potting.
本发明中,带土上盆时,进一步优选尽量减少对根部的伤害。例如,带大土坨起苗,然后进行根部修剪去除明显的烂根、病根。上盆时所留的根的长度可以是35-40cm,例如为35cm、36cm、37cm、38cm、39cm或40cm,如果根系保留过长,则上盆困难,影响盆栽管理;如果根系保留过短,上盆后难以吸收水分无法供给枝条足够养分,影响成花。在起苗时,最好保证留有护心土,这样可以及时供给牡丹开花所需的养分,有益于其根部的后期生长。In the present invention, when potting with soil, it is further preferred to minimize the damage to the roots. For example, raise seedlings with a large soil lump, and then perform root pruning to remove obvious rotten and diseased roots. The length of the root left when potting can be 35-40cm, such as 35cm, 36cm, 37cm, 38cm, 39cm or 40cm. If the root system is kept too long, it will be difficult to pot and affect potting management; if the root system is kept too short, After being potted, it is difficult to absorb water and cannot supply enough nutrients to the branches, which affects flower formation. When raising seedlings, it is best to ensure that the core soil is left, so that the nutrients needed for peony flowering can be supplied in time, which is beneficial to the later growth of its roots.
本发明对所用的花盆也没有特别的限制,例如可以根据根冠高度和直径以及地上部的高度和直径来选择具有适当高度和直径的花盆。一般情况下,可以采用例如高度为40cm~50cm(例如为40cm、45cm或50cm)、直径为30cm~60cm(例如为30cm、40cm、50cm或60cm)的花盆,如果花盆尺寸(高度和/或直径)过小,则可能无法将牡丹植株的地下部埋入栽培基质中,或者影响根部的进一步生长发育;如果尺寸过大,则可能与整个牡丹植株的比例不协调而有失美观。The present invention has no special limitation on the used flowerpots, for example, a flowerpot with an appropriate height and diameter can be selected according to the height and diameter of the root cap and the height and diameter of the aboveground part. In general, flowerpots with a height of 40cm to 50cm (such as 40cm, 45cm or 50cm) and a diameter of 30cm to 60cm (such as 30cm, 40cm, 50cm or 60cm) can be used, if the flowerpot size (height and/or or diameter) is too small, it may not be possible to bury the underground part of the peony plant in the cultivation medium, or affect the further growth and development of the root; if the size is too large, it may be out of harmony with the proportion of the whole peony plant and lose the appearance.
本发明的上盆所用的基质没有特别限制,例如可以采用包含草炭和珍珠岩的混合基质。The matrix used in the upper basin of the present invention is not particularly limited, for example, a mixed matrix comprising peat and perlite can be used.
在上盆后,可以采用常规的栽培方式对盆栽牡丹进行管理。After potting, the potted peonies can be managed by conventional cultivation methods.
在另外一些优选的实施方式中,在所述脱叶处理之后并且在所述涂抹处理之前,对经脱叶处理的植株进行10至15天的控水处理,例如进行10天、12天、14天或15天的控水处理。所谓控水,即不通过人为方式补充水分并且避免植株接受自然降水的补充。In some other preferred embodiments, after the defoliation treatment and before the smear treatment, the defoliated plants are subjected to water control treatment for 10 to 15 days, for example, 10 days, 12 days, 14 days Day or 15 days of water control treatment. The so-called water control means not supplementing water through artificial means and avoiding the supplement of natural precipitation for plants.
在一些优选的实施方式中,在所述控水处理结束后至上盆前,还对盆栽的秋发牡丹进行浇透水处理。在另外一些优选的实施方式中,优选在这一段时间内每两周浇一次透水,进一步优选同时将环境的空气相对湿度保持70%-80%,例如75%的相对湿度。In some preferred embodiments, after the water control treatment is completed and before being placed in the pot, the potted peonies are also watered thoroughly. In some other preferred embodiments, it is preferred to pour water once every two weeks during this period of time, and it is further preferred to keep the relative air humidity of the environment at 70%-80%, such as 75%, at the same time.
在一些实施方式中,优选在上盆之后进行缓苗处理。例如,将上盆后的植株于阴凉处放置3-5天,同时,昼夜温度优选不高于15℃。如果昼夜温度过高,容易造成叶片生长过快,争夺花蕾养分而影响成花。In some embodiments, it is preferable to carry out slow seedling treatment after potting. For example, place the potted plants in a cool place for 3-5 days, and at the same time, the day and night temperature is preferably not higher than 15°C. If the day and night temperature is too high, it is easy to cause the leaves to grow too fast, compete for the nutrients of the flower buds and affect the flower formation.
在一些优选的实施方式中,在缓苗过程中,环境相对湿度保持在80%-90%,例如80%、82%、84%、86%、88%或90%。如果在缓苗的过程中如果相对湿度过低,容易造成植株萎蔫,不利于花蕾发育进而影响成花。In some preferred embodiments, during the seedling slowing process, the relative humidity of the environment is maintained at 80%-90%, such as 80%, 82%, 84%, 86%, 88% or 90%. If the relative humidity is too low in the process of slowing down seedlings, it is easy to cause the plants to wilt, which is not conducive to the development of flower buds and thus affects flower formation.
在本发明的一些优选的实施方式中,为了进一步提高盆栽牡丹的商品性,在缓苗处理之后,还对盆栽秋发牡丹进行炼苗处理。炼苗的时候没有特别限制,例如可以进行为期3至5天的炼苗处理。采用本发明方法栽培秋发牡丹,在炼苗结束后,可以使盆栽牡丹在国庆节期间开放。In some preferred embodiments of the present invention, in order to further improve the commerciality of the potted peonies, after the seedling slowing treatment, the potted peonies are also subjected to seedling hardening treatment. There is no special restriction on the hardening of seedlings, for example, the hardening of seedlings can be carried out for 3 to 5 days. By adopting the method of the invention to cultivate the autumn peony, the potted peony can be opened during the National Day after the seedling hardening is finished.
下文将通过实施例来对本发明的具体实施方式进行举例说明,但是这些实施例不应该解释为本发明仅限于这些实施例。Hereinafter, examples will be used to illustrate specific embodiments of the present invention, but these examples should not be construed as limiting the present invention to these examples.
实施例Example
实施例1至15Examples 1 to 15
实验地点为中国农业科学院蔬菜花卉研究所牡丹资源圃。在2011年8月10日,选取20株4-5年生、具有8-10个枝条生长健壮的秋发牡丹品种‘藏枝红’(paeoniasuffruticosa‘cangzhihong’),进行全株脱叶处理。脱叶后,控水10天,然后抹去侧芽,只保留最靠近顶端的一个芽(一般为顶芽或第一位芽),每个实施例采用10株。在8月25号对所保留的芽进行第一次秋发素(其中包含的活性试剂GA3、生长素和玉米素均为分析纯,购于北京化学试剂公司,浓度如表1所示)涂抹处理。The experimental site is the Peony Resource Garden of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. On August 10, 2011, 20 4-5-year-old autumn peony cultivar 'Cangzhihong' (paeoniasuffruticosa 'cangzhihong') with 8-10 vigorous branches were selected for defoliation of the whole plant. After defoliation, control water for 10 days, then wipe off the side buds, only keep a bud (generally the top bud or the first bud) closest to the top, and each embodiment adopts 10 plants. On August 25th, the retained buds were subjected to the first Qiufasu (the active reagents GA 3 , auxin and zeatin included in it were all analytically pure, purchased from Beijing Chemical Reagent Company, and the concentrations were as shown in Table 1) Smudge treatment.
待牡丹花蕾进入透色期后,于下午16-17点钟(阳光减弱后)起苗,以防止水分蒸发过快。起苗时带上护心土,以减少对根系的伤害。起苗后对根系进行修剪,去除病根、烂根,保留根长约38cm。将修剪后的植株栽植于高度为45cm,直径为36cm的花盆中。填充基质(草炭:珍珠岩=2∶1(体积比))并立即浇灌透水。将植株放于遮阴处,每天于上午、中午、下午进行三次喷水,使环境相对湿度保持在70%~80%,在该条件下进行4天的缓苗处理,以控制牡丹在国庆节期间开花。After the peony buds enter the translucent period, the seedlings should be raised at 16-17 o'clock in the afternoon (after the sun has weakened) to prevent the water from evaporating too quickly. Take protective soil when raising seedlings to reduce damage to the root system. After the seedlings are raised, the root system is trimmed to remove diseased and rotten roots, and the root length is about 38 cm. Plant the pruned plants in a flower pot with a height of 45 cm and a diameter of 36 cm. Fill the matrix (peat: perlite = 2:1 (volume ratio)) and water it immediately. Put the plants in a shady place and spray water three times a day in the morning, noon, and afternoon to keep the relative humidity of the environment at 70% to 80%. During flowering.
表1在以‘藏枝红’品种进行国庆促成栽培处理的参数数值以及所测定的参数的测定结果Table 1. The parameter values and the measured parameters of the 'Zangzhihong' variety for the National Day promotion cultivation treatment
由表1结果可以看出,当秋发素成分中只有GA3时,在GA3浓度为800mg/L时成花效果较好;在GA3中加入玉米素时,效果和只含有GA3效果相差无几,当玉米素和生长素(IAA)配合使用时,有显著促进成花作用。当秋发素内含有GA3、IAA和玉米素且三者比例接近8∶1∶1时,可以达到很好的促成栽培效果。It can be seen from the results in Table 1 that when only GA 3 is included in the composition of Qiufasu, the flowering effect is better when the concentration of GA 3 is 800mg/L; when zeatin is added to GA 3 , the effect is different from that only containing GA3 Few, when zeatin and auxin (IAA) are used together, it can significantly promote flower formation. When the autumn hair contains GA 3 , IAA and zeatin and the ratio of the three is close to 8:1:1, it can achieve a good effect of promoting cultivation.
实施例16Example 16
实验地点为中国农业科学院蔬菜花卉研究所牡丹资源圃。在2011年8月10日,选取20株4-5年生、具有8-10个枝条生长健壮的秋发牡丹品种‘藏枝红’(paeoniasuffruticosa‘cangzhihong’),进行全株脱叶处理。脱叶后,控水10天,然后抹去侧芽,只保留最靠近顶端的一个芽(一般为顶芽或第一位芽)。在8月25号对所保留的芽进行第一次秋发素(其中包含的活性试剂GA3、生长素和玉米素均为分析纯,购于北京化学试剂公司,GA3、生长素和玉米素重量比为8∶1∶1,下同)涂抹处理,第一次涂抹所用的秋发素的浓度(即GA3、生长素和玉米素的浓度之和)为1000mg/L。此后每隔3天依次进行两次涂抹,即进行第二次涂抹和第三次涂抹,第二次涂抹和第三次涂抹所用的秋发素的浓度分别为1500mg/L和2000mg/L。The experimental site is the Peony Resource Garden of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. On August 10, 2011, 20 4-5-year-old autumn peony cultivar 'Cangzhihong' (paeoniasuffruticosa 'cangzhihong') with 8-10 vigorous branches were selected for defoliation of the whole plant. After defoliation, control the water for 10 days, then wipe off the side buds, and only keep the bud closest to the top (usually the top bud or the first bud). On August 25th, the retained buds were tested for the first time for autumn hair (the active reagents GA 3 , auxin and zeatin contained in it were all analytically pure, purchased from Beijing Chemical Reagent Company, GA 3 , auxin and corn The weight ratio of auxin and zeatin is 8:1:1, the same below), and the concentration of qiufalin (that is, the sum of the concentrations of GA 3 , auxin and zeatin) used for the first application is 1000 mg/L. Thereafter, two smears were carried out successively every 3 days, that is, the second smear and the third smear, and the concentrations of the autumn fat used in the second smear and the third smear were 1500 mg/L and 2000 mg/L respectively.
待牡丹花蕾进入透色期后,于下午16-17点钟(阳光减弱后)起苗,以防止水分蒸发过快。起苗时带上护心土,以减少对根系的伤害。起苗后对根系进行修剪,去除病根、烂根,保留根长约38cm。将修剪后的植株栽植于高度为45cm,直径为36cm的花盆中。填充基质(草炭:珍珠岩=2∶1(体积比))并立即浇灌透水。将植株放于遮阴处,每天于上午、中午、下午进行三次喷水,使环境相对湿度保持在70%~80%,在该条件下进行4天的缓苗处理,从而控制牡丹在国庆节期间开花。After the peony buds enter the translucent period, the seedlings should be raised at 16-17 o'clock in the afternoon (after the sun has weakened) to prevent the water from evaporating too quickly. Take protective soil when raising seedlings to reduce damage to the root system. After the seedlings are raised, the root system is trimmed to remove diseased and rotten roots, and the root length is about 38 cm. Plant the pruned plants in a flower pot with a height of 45 cm and a diameter of 36 cm. Fill the matrix (peat: perlite = 2:1 (volume ratio)) and water it immediately. Put the plants in a shady place and spray water three times a day in the morning, noon and afternoon to keep the relative humidity of the environment at 70% to 80%. During flowering.
实施例17至23Examples 17 to 23
除表2中所列的内容之外,以与实施例16相同的方式进行栽培。Cultivation was performed in the same manner as in Example 16 except for the contents listed in Table 2.
表2在以‘藏枝红’品种进行国庆开花促成栽培的试验中所采用的参数和所测得的参数Table 2 The parameters used and measured parameters in the experiment of promoting national day flowering with the variety 'Zangzhihong'
从表1和表2结果可以看出,本发明通过脱叶处理可以打破秋发牡丹的芽休眠,使之萌发,但并不能促使混合芽全部成花。对于秋发牡丹而言,脱叶处理配合浓度逐渐提高的秋发素涂抹处理,使得秋发牡丹在打破休眠、提高成花率和缩短生育期方面均有显著效果。As can be seen from the results in Table 1 and Table 2, the present invention can break the dormancy of the buds of the peony in autumn and make it germinate by defoliation treatment, but it cannot impel all the mixed buds to flower. For Qiufa peony, the defoliation treatment combined with the gradually increasing concentration of Qiufasu smear treatment makes Qiufa peony have significant effects in breaking dormancy, increasing flowering rate and shortening the growth period.
实施例24至31Examples 24 to 31
除了表3中所列的内容并采用‘西瓜瓤’品种代替‘藏枝红’品种之外,按照与实施例16相同的方式进行。Except the content listed in table 3 and adopting ' watermelon flesh ' kind to replace ' Zangzhihong' kind, carry out according to the mode identical with embodiment 16.
表3在‘西瓜瓤’品种进行国庆开花促成栽培的试验中所采用的参数和所测得的参数Table 3 The parameters used and the measured parameters in the test of the 'Watermelon Flesh' variety to promote the cultivation of National Day flowering
实施例32至38Examples 32 to 38
除了表4中所示内容之外,采用与实施例16相同的方式进行。Except for the contents shown in Table 4, it was carried out in the same manner as in Example 16.
表4在以‘藏枝红’品种进行国庆促成栽培处理的参数数值以及所测定的参数的测定结果Table 4 The parameter values and the measured parameters of the national day promoting cultivation treatment with the 'Zangzhihong' variety
以上各表中的各指标的测定方法如下:The measurement methods of each index in the above tables are as follows:
萌发率(%)=萌发的芽数/人工处理的芽数×100%Germination rate (%) = number of germinated buds / number of artificially treated buds × 100%
成花率(%)=成花数/人工处理的芽数×100%Flowering rate (%) = number of flowers / number of artificially treated buds × 100%
败育率(%)=败育数/人工处理的芽数×100%Abortion rate (%) = number of abortions / number of artificially treated buds × 100%
株成花率(%)=开花植株数/处理植株数×100%Plant flowering rate (%) = number of flowering plants / number of treated plants × 100%
生育期(d)=从处理到进入盛花期的天数Growth period (d) = days from treatment to full flowering
盛花期(d)=花朵开到最大直径并持续开放的天数Full flowering period (d) = number of days for flowers to reach maximum diameter and remain open
由表4结果可以看出,本发明所述带“护心土”并保留35-40cm根长上盆在促进牡丹萌发以及促进成花方面有显著作用。当保留根长在45cm以上时也可以保证较好的成花及萌发率,但是根长的加大增加了操作的难度,例如需要较大的花盆可能会使植株与花盆比例不协调而影响美观。因此,从操作性的角度考虑,采用35-40cm的根长更为适宜。As can be seen from the results in Table 4, the pots with "heart-protecting soil" and retaining 35-40cm root length of the present invention have a significant effect on promoting peony germination and flower formation. When the root length is kept above 45cm, better flower formation and germination rate can be guaranteed, but the increase of root length increases the difficulty of operation. For example, the need for larger flower pots may cause the proportion of plants and flower pots to be uncoordinated. Affect the appearance. Therefore, from the operational point of view, it is more appropriate to use a root length of 35-40 cm.
本文中所描述的实施例仅仅是对本发明的优选实施例所作的举例说明。本发明所属技术领域的技术人员可以根据本发明所公开的内容对所描述的实施例进行各种修改,但是由此修改而得到的没有偏离本发明的实质的技术方案仍然落在所附权利要求书所限定的范围之内。The embodiments described herein are merely illustrative of preferred embodiments of the invention. Those skilled in the art to which the present invention belongs can carry out various modifications to the described embodiments according to the disclosed content of the present invention, but the technical solutions obtained by such modifications without departing from the essence of the present invention still fall within the appended claims within the bounds of the book.
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CN103444397A (en) * | 2013-08-16 | 2013-12-18 | 苏州仁成生物科技有限公司 | Method for promoting peony flower cultivation |
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CN104686150B (en) * | 2015-03-04 | 2017-03-01 | 甘肃农业大学 | A kind of florescence control method promoting Paeonia papaveracea Winter blooming |
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