CN103426354B - A kind of whole-body muscle Slide processing of adult dogs - Google Patents
A kind of whole-body muscle Slide processing of adult dogs Download PDFInfo
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Abstract
The muscle specimen manufacture method of a kind of adult dogs, after selecting anesthesia, through the corpse of the adult dogs that perfusion fixation is put to death；Then adult dogs is carried out peeling, and completely isolates dog whole-body muscle；After isolated dog muscle water is cleaned up, with the ethanol water of 88%, soaking two days later, taking-up water is rinsed well, then with the dehydrated alcohol that volume ratio is 1.5:2 and dimethylbenzene mixed solution, soak and take out two days later, it is placed on dark ventilation to dry in the air 6 days, is placed on afterwards in the hydrogen peroxide after dilution, takes out after soaking 46 days, rinse well with clear water, dry；Then with glue, muscle is fixed on a glass, posture when making muscle keep dog to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of adult dogs.Preparation method of the present invention is simple, with low cost, the muscle specimen of prepared adult dogs, it is easy to preserving, structural integrity is attractive in appearance, and morphologic localization understands nature.
Description
Technical field
The present invention relates to a kind of Slide processing, the whole-body muscle Slide processing of a kind of adult dogs.
Background technology
Zoological specimens, be to maintain former state in kind or through arrangement be available for study, research time referential can be as the individuality represented in one species, it is possible to as the important references of the universal publicity of scientific research, teaching and Sci-tech Knowledge.Scientific value, ornamental value and the reserve value that what animal and tissue specimen contained can not be substituted, other arts work of erect image equally enter into family, become the indoor new ornament of people.One makes exquisite specimen and not only brings people's knowledge, the most also brings people the enjoyment of art.Perfect animal muscle specimen is a vivid practical teaching aid, can be again a consummate art work, and it represents colleges and universities, the level of development of a subject and grasps the professional skill of this disciplinary study person.Preparation of specimen is also a research work, in manufacturing process, can constantly discover problems and solve them, and creates new technology, can broaden one's knowledge simultaneously, tempers thinking and the manipulative ability of participant.It is fabricated to whole muscle specimen, whole-body muscle structure can be demonstrated, specimen structure is attractive in appearance, form understands nature, not only as directly perceived, vivid teaching aid in " animal anatomy " imparts knowledge to students, and student can be allowed in manufacturing process to participate in, allow student understand and grasp preparation of specimen's process, simultaneously the starting of training student, beat one's brains ability.Contribute to, to student's understanding to animal whole-body muscle overview, the contour structures of whole-body muscle being grasped, the understanding of the Name & Location relation of muscle is all played well help, is favorably improved teaching efficiency.
Summary of the invention
The present invention seeks to as solving above-mentioned technical problem, it is provided that the muscle specimen manufacture method of a kind of adult dogs, its preparation method is simple, and prepared specimen can demonstrate the whole-body muscle of adult dogs, and structure is attractive in appearance, and form understands nature.
A kind of muscle specimen manufacture method of adult dogs, its concrete preparation process includes:
Step one, the corpse of selection adult dogs；
Step 2, peeling: faced upward by dog corpse and be put on dissecting table, cut the osculum of a diameter of 1-2cm respectively inside front carpal joint and hind leg tarsal joint with operating scissors, cuts off dog skin the most respectively to anus front inside cut osculum；It is then peeled off dog skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and mouth skin of lip, skin is completely stripped, finally gills；
Step 3, the whole-body muscle of separation dog: isolate the muscle of dog whole body with scalpel；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis；At cervical region separation trapezius muscle, omoplate transverse process flesh, longissimus cervicis, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off fascia, connective tissue and blood vessel along the boundary of muscle, terminal, separate on latissimus dorsi m., chest muscle, veutro serratus, Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate the muscle such as flesh, latissimus dorsi m., Intercostal muscle, back of the body waist musculus longissimus, iliocostalis, psoas major, psoas minor, iliocostalis ditch, dorsal part serratus, intercostales externi, intercostales interni, obliquus externus abdominis m., obliquus internus abdominis m., rectus abdominis m., transversus abdominis m..At forelimb, separate supraspinatus, infraspinatus, triangular muscle, subscapularis, teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps muscle, arm flesh, arm biceps, forearm, forward foot in a step flesh；At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, buttocks biceps femoris, semitendinosus m., semimembranosus m., tensor fasciae latae, quadriceps femoris, gracilis, adductor, shank and rear foot flesh etc.；
Step 4, defat: by isolated dog muscle specimen, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1.5:2, dehydrated alcohol and dimethylbenzene mixed solution is made not to have dog muscle completely, changed every 1-2 days time and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1.5:2, soak and take out two days later；Or isolated dog muscle is placed in leaching container, pours gasoline into, airtight, soak about 6-7 days and carry out defat.During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 5, defat: the dog muscle after defat is placed on dark ventilation and dries in the air 6 days, then the detergent solution that dog muscle mass percent is 5%-7% dried well is soaked 1-2h, then use clean water three times；
Described mass percent is that the preparation method of the detergent solution of 5%-7% is: weigh detergent, adds distilled water, prepares the detergent solution that mass percent is 5%-7%, matching while using or be placed in 4 DEG C of preservations；
Step 6, above-mentioned steps five is processed after dog muscle insert the hydrogen peroxide after dilution, take out after soaking 4-6 days, rinse well with clear water, dry；
The preparation method of the described hydrogen peroxide after dilution is: hydrogen peroxide is diluted to the solution that mass percent is 4% of hydrogen peroxide with distilled water, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 DEG C of preservations；
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep dog to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of adult dogs.
Provide the benefit that:
1, the muscle specimen manufacture method of adult dogs of the present invention, preparation method is simple, easy, with low cost；Using ethanol water, dehydrated alcohol and dimethylbenzene or gasoline that dog muscle has been carried out ungrease treatment, defat is thorough, can prevent from using permanent and preserving, slowly oozing out of dog muscle oils and fats, pollute mouldy, make specimen impaired, hinders use and attractive in appearance.After using the dilution that mass percent is 4% of hydrogen oxide, hydrogen peroxide is bleached, and effect is preferably and to dog muscle specimen not damaged.
2, the muscle specimen manufacture method of adult dogs of the present invention, the muscle specimen of prepared dog, it is easy to preserve, structural integrity is attractive in appearance, morphologic localization understands that nature, whole-body muscle are all clearly illustrated, each piece of muscle represents the most well and is fixed on the natural place of dog body.Solve and be difficult to the clear problem differentiating dog whole-body muscle.Muscle is the emphasis in Teaching of Anatomy, the mainly theory of the muscle in systematic anatomy teaching, and topologic Students in Teaching does not dissect whole muscle；The present invention preferably resolves this difficult problem.Excite again student learning interest, improve teaching efficiency.
Detailed description of the invention
A kind of muscle specimen manufacture method of adult dogs, its concrete preparation process includes:
Step one, the corpse of selection adult dogs；
Step 2, peeling: faced upward by dog corpse and be put on dissecting table, cut the osculum of a diameter of 1-2cm respectively inside front carpal joint and hind leg tarsal joint with operating scissors, cuts off dog skin the most respectively to anus front inside cut osculum；It is then peeled off dog skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and mouth skin of lip, skin is completely stripped, finally gills；
Step 3, the whole-body muscle of separation dog: isolate the muscle of dog whole body with scalpel；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis；At cervical region separation trapezius muscle, omoplate transverse process flesh, longissimus cervicis, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off fascia, connective tissue and blood vessel along the boundary of muscle, terminal, separate on latissimus dorsi m., chest muscle, veutro serratus, Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate the muscle such as flesh, latissimus dorsi m., Intercostal muscle, back of the body waist musculus longissimus, iliocostalis, psoas major, psoas minor, iliocostalis ditch, dorsal part serratus, intercostales externi, intercostales interni, obliquus externus abdominis m., obliquus internus abdominis m., rectus abdominis m., transversus abdominis m..At forelimb, separate supraspinatus, infraspinatus, triangular muscle, subscapularis, teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps muscle, arm flesh, arm biceps, forearm, forward foot in a step flesh；At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, buttocks biceps femoris, semitendinosus m., semimembranosus m., tensor fasciae latae, quadriceps femoris, gracilis, adductor, shank and rear foot flesh etc.；
Step 4, defat: by isolated dog muscle specimen, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1.5:2, dehydrated alcohol and dimethylbenzene mixed solution is made not to have dog muscle completely, changed every 1-2 days time and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1.5:2, soak and take out two days later；Or isolated dog muscle is placed in leaching container, pours gasoline into, airtight, soak about 6-7 days and carry out defat.During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 5, defat: the dog muscle after defat is placed on dark ventilation and dries in the air 6 days, then the detergent solution that dog muscle mass percent is 5%-7% dried well is soaked 1-2h, then use clean water three times；
Described mass percent is that the preparation method of the detergent solution of 5%-7% is: weigh detergent, adds distilled water, prepares the detergent solution that mass percent is 5%-7%, matching while using or be placed in 4 DEG C of preservations；
Step 6, above-mentioned steps five is processed after dog muscle insert the hydrogen peroxide after dilution, take out after soaking 4-6 days, rinse well with clear water, dry；
The preparation method of the described hydrogen peroxide after dilution is: hydrogen peroxide is diluted to the solution that mass percent is 4% of hydrogen peroxide with distilled water, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 DEG C of preservations；
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep dog to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of adult dogs.
Following example can make those skilled in the art understand the present invention comprehensively, but limits the present invention never in any form.
Embodiment
1
A kind of muscle specimen manufacture method of adult dogs, its concrete preparation process includes:
Step one, the corpse of selection adult dogs；
Step 2, peeling: faced upward by dog corpse and be put on dissecting table, cut the osculum of a diameter of 1cm respectively inside front carpal joint and hind leg tarsal joint with operating scissors, cuts off dog skin the most respectively to anus front inside cut osculum；It is then peeled off dog skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and mouth skin of lip, skin is completely stripped, finally gills；
Step 3, the whole-body muscle of separation dog: isolate the muscle of dog whole body with scalpel；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis；At cervical region separation trapezius muscle, omoplate transverse process flesh, longissimus cervicis, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off fascia, connective tissue and blood vessel along the boundary of muscle, terminal, separate on latissimus dorsi m., chest muscle, veutro serratus, Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate the muscle such as flesh, latissimus dorsi m., Intercostal muscle, back of the body waist musculus longissimus, iliocostalis, psoas major, psoas minor, iliocostalis ditch, dorsal part serratus, intercostales externi, intercostales interni, obliquus externus abdominis m., obliquus internus abdominis m., rectus abdominis m., transversus abdominis m..At forelimb, separate supraspinatus, infraspinatus, triangular muscle, subscapularis, teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps muscle, arm flesh, arm biceps, forearm, forward foot in a step flesh；At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, buttocks biceps femoris, semitendinosus m., semimembranosus m., tensor fasciae latae, quadriceps femoris, gracilis, adductor, shank and rear foot flesh etc.；
Step 4, defat: by isolated dog muscle specimen, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1.5:2, dehydrated alcohol and dimethylbenzene mixed solution is made not to have dog muscle completely, changed every 1 day time and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1.5:2, soak and take out two days later；During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 5, defat: the dog muscle after defat is placed on dark ventilation and dries in the air 6 days, then the detergent solution that dog muscle mass percent is 5% dried well is soaked 1h, then use clean water three times；
Described mass percent is that the preparation method of the detergent solution of 5% is: weigh detergent, adds distilled water, prepares the detergent solution that mass percent is 5%, matching while using or be placed in 4 DEG C of preservations；
Step 6, above-mentioned steps five is processed after dog muscle insert the hydrogen peroxide after dilution, take out after soaking 4-6 days, rinse well with clear water, dry；
The preparation method of the described hydrogen peroxide after dilution is: hydrogen peroxide is diluted to the solution that mass percent is 4% of hydrogen peroxide with distilled water, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 DEG C of preservations；
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep dog to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of adult dogs.
Embodiment
2
Step one, the corpse of selection adult dogs；
Step 2, peeling: faced upward by dog corpse and be put on dissecting table, cut the osculum of a diameter of 2cm respectively inside front carpal joint and hind leg tarsal joint with operating scissors, cuts off dog skin the most respectively to anus front inside cut osculum；It is then peeled off dog skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and mouth skin of lip, skin is completely stripped, finally gills；
Step 3, the whole-body muscle of separation dog: isolate the muscle of dog whole body with scalpel；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis；At cervical region separation trapezius muscle, omoplate transverse process flesh, longissimus cervicis, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off fascia, connective tissue and blood vessel along the boundary of muscle, terminal, separate on latissimus dorsi m., chest muscle, veutro serratus, Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate the muscle such as flesh, latissimus dorsi m., Intercostal muscle, back of the body waist musculus longissimus, iliocostalis, psoas major, psoas minor, iliocostalis ditch, dorsal part serratus, intercostales externi, intercostales interni, obliquus externus abdominis m., obliquus internus abdominis m., rectus abdominis m., transversus abdominis m..At forelimb, separate supraspinatus, infraspinatus, triangular muscle, subscapularis, teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps muscle, arm flesh, arm biceps, forearm, forward foot in a step flesh；At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, buttocks biceps femoris, semitendinosus m., semimembranosus m., tensor fasciae latae, quadriceps femoris, gracilis, adductor, shank and rear foot flesh etc.；
Step 4, defat: by isolated dog muscle specimen, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1.5:2, dehydrated alcohol and dimethylbenzene mixed solution is made not to have dog muscle completely, changed every 2 days time and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1.5:2, soak and take out two days later；During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 5, defat: the dog muscle after defat is placed on dark ventilation and dries in the air 6 days, then the detergent solution that dog muscle mass percent is 7% dried well is soaked 2h, then use clean water three times；
Described mass percent is that the preparation method of the detergent solution of 7% is: weigh detergent, adds distilled water, prepares the detergent solution that mass percent is 7%, matching while using or be placed in 4 DEG C of preservations；
Step 6, above-mentioned steps five is processed after dog muscle insert the hydrogen peroxide after dilution, take out after soaking 6 days, rinse well with clear water, dry；
The preparation method of the described hydrogen peroxide after dilution is: hydrogen peroxide is diluted to the solution that mass percent is 4% of hydrogen peroxide with distilled water, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 DEG C of preservations；
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep dog to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of adult dogs.
Embodiment
3
Step one, the corpse of selection adult dogs；
Step 2, peeling: faced upward by dog corpse and be put on dissecting table, cut the osculum of a diameter of 1.5cm respectively inside front carpal joint and hind leg tarsal joint with operating scissors, cuts off dog skin the most respectively to anus front inside cut osculum；It is then peeled off dog skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and mouth skin of lip, skin is completely stripped, finally gills；
Step 3, the whole-body muscle of separation dog: isolate the muscle of dog whole body with scalpel；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis；At cervical region separation trapezius muscle, omoplate transverse process flesh, longissimus cervicis, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off fascia, connective tissue and blood vessel along the boundary of muscle, terminal, separate on latissimus dorsi m., chest muscle, veutro serratus, Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate the muscle such as flesh, latissimus dorsi m., Intercostal muscle, back of the body waist musculus longissimus, iliocostalis, psoas major, psoas minor, iliocostalis ditch, dorsal part serratus, intercostales externi, intercostales interni, obliquus externus abdominis m., obliquus internus abdominis m., rectus abdominis m., transversus abdominis m..At forelimb, separate supraspinatus, infraspinatus, triangular muscle, subscapularis, teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps muscle, arm flesh, arm biceps, forearm, forward foot in a step flesh；At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, buttocks biceps femoris, semitendinosus m., semimembranosus m., tensor fasciae latae, quadriceps femoris, gracilis, adductor, shank and rear foot flesh etc.；
Step 4, defat: by isolated dog muscle specimen, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1.5:2, dehydrated alcohol and dimethylbenzene mixed solution is made not to have dog muscle completely, changed every 1 day time and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1.5:2, soak and take out two days later；During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 5, defat: the dog muscle after defat is placed on dark ventilation and dries in the air 6 days, then the detergent solution that dog muscle mass percent is 6% dried well is soaked 1.5h, then use clean water three times；
Described mass percent is that the preparation method of the detergent solution of 6% is: weigh detergent, adds distilled water, prepares the detergent solution that mass percent is 6%, matching while using or be placed in 4 DEG C of preservations；
Step 6, above-mentioned steps five is processed after dog muscle insert the hydrogen peroxide after dilution, take out after soaking 4-6 days, rinse well with clear water, dry；
The preparation method of the described hydrogen peroxide after dilution is: hydrogen peroxide is diluted to the solution that mass percent is 4% of hydrogen peroxide with distilled water, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 DEG C of preservations；
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep dog to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of adult dogs.
Embodiment
4
Step one, the corpse of selection adult dogs；
Step 2, peeling: faced upward by dog corpse and be put on dissecting table, cut the osculum of a diameter of 1.3cm respectively inside front carpal joint and hind leg tarsal joint with operating scissors, cuts off dog skin the most respectively to anus front inside cut osculum；It is then peeled off dog skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and mouth skin of lip, skin is completely stripped, finally gills；
Step 3, the whole-body muscle of separation dog: isolate the muscle of dog whole body with scalpel；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis；At cervical region separation trapezius muscle, omoplate transverse process flesh, longissimus cervicis, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off fascia, connective tissue and blood vessel along the boundary of muscle, terminal, separate on latissimus dorsi m., chest muscle, veutro serratus, Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate the muscle such as flesh, latissimus dorsi m., Intercostal muscle, back of the body waist musculus longissimus, iliocostalis, psoas major, psoas minor, iliocostalis ditch, dorsal part serratus, intercostales externi, intercostales interni, obliquus externus abdominis m., obliquus internus abdominis m., rectus abdominis m., transversus abdominis m..At forelimb, separate supraspinatus, infraspinatus, triangular muscle, subscapularis, teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps muscle, arm flesh, arm biceps, forearm, forward foot in a step flesh；At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, buttocks biceps femoris, semitendinosus m., semimembranosus m., tensor fasciae latae, quadriceps femoris, gracilis, adductor, shank and rear foot flesh etc.；
Step 4, defat: by isolated dog muscle specimen, rinse well with water, be placed in leaching container, pour gasoline into, airtight, soaks and carries out defat in about 7 days.During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 5, defat: the dog muscle after defat is placed on dark ventilation and dries in the air 6 days, then the detergent solution that dog muscle mass percent is 7% dried well is soaked 2h, then use clean water three times；
Described mass percent is that the preparation method of the detergent solution of 5.5% is: weigh detergent, adds distilled water, prepares the detergent solution that mass percent is 5.5%, matching while using or be placed in 4 DEG C of preservations；
Step 6, above-mentioned steps five is processed after dog muscle insert the hydrogen peroxide after dilution, take out after soaking 5 days, rinse well with clear water, dry；
The preparation method of the described hydrogen peroxide after dilution is: hydrogen peroxide is diluted to the solution that mass percent is 4% of hydrogen peroxide with distilled water, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 DEG C of preservations；
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep dog to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of adult dogs.
Claims (2)
1. the whole-body muscle Slide processing of an adult dogs, it is characterised in that: its concrete preparation process includes:
Step one, the corpse of selection adult dogs；
Step 2, peeling: faced upward by dog corpse and be put on dissecting table, cut the osculum of a diameter of 1-2cm respectively inside front carpal joint and hind leg tarsal joint with operating scissors, cuts off dog skin the most respectively to anus front inside cut osculum；It is then peeled off dog skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and mouth skin of lip, skin is completely stripped, finally gills；
Step 3, the whole-body muscle of separation dog: isolate the muscle of dog whole body with scalpel；
Step 4, defat: by isolated dog muscle specimen, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1.5:2, dehydrated alcohol and dimethylbenzene mixed solution is made not to have dog muscle completely, changed every 1-2 days time and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1.5:2, soak and take out two days later；In the operating process of described defat, obturage leaching container with Polypropylence Sheet, prevent solution evaporation；
Process after step 5, defat: the dog muscle after defat is placed on dark ventilation and dries in the air 6 days, then the detergent solution that dog muscle mass percent is 5%-7% dried well is soaked 1-2h, then use clean water three times；
Described mass percent is that the preparation method of the detergent solution of 5%-7% is: weigh detergent, adds distilled water, prepares the detergent solution that mass percent is 5%-7%, matching while using or be placed in 4 DEG C of preservations；
Step 6, above-mentioned steps five is processed after dog muscle insert the hydrogen peroxide after dilution, take out after soaking 4-6 days, rinse well with clear water, dry；
The preparation method of the described hydrogen peroxide after dilution is: hydrogen peroxide is diluted to the solution that mass percent is 4% of hydrogen peroxide with distilled water, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 DEG C of preservations；
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep dog to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of adult dogs.
The whole-body muscle Slide processing of a kind of adult dogs the most as claimed in claim 1, it is characterized in that: the described degreasing method in step 4 uses in an alternative manner: isolated dog muscle specimen is placed in leaching container, pour gasoline into, airtight, soak and carry out defat in 5 days.
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| CN103824499B (en) * | 2014-01-29 | 2016-05-11 | 河南科技大学 | A kind of preparation method of adult rabbits sample |
| CN105355127B (en) * | 2015-12-03 | 2017-10-17 | 河南科技大学 | A kind of adult rat tail vein injection model production method based on mathematical modeling |
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