CN103426354A - Method for preparing whole-body muscle specimen of adult dog - Google Patents
Method for preparing whole-body muscle specimen of adult dog Download PDFInfo
- Publication number
- CN103426354A CN103426354A CN2013103613385A CN201310361338A CN103426354A CN 103426354 A CN103426354 A CN 103426354A CN 2013103613385 A CN2013103613385 A CN 2013103613385A CN 201310361338 A CN201310361338 A CN 201310361338A CN 103426354 A CN103426354 A CN 103426354A
- Authority
- CN
- China
- Prior art keywords
- muscle
- dog
- muscles
- days
- whole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A method for preparing a whole-body muscle specimen of an adult dog includes the steps: fixing the corpse of the killed adult dog by perfusion after selecting anesthesia; peeling off the skin of the adult dog and completely separating out whole-body muscles of the dog; cleaning up the separated muscles of the dog with water, soaking the muscles by 88% of ethanol water solution for two days, taking out the muscles, washing out the muscles with water, soaking the muscles by mixed solution of absolute ethanol and xylene with the volume ratio of 1.5:2 for two days, taking out the muscles, airing the muscles at a dark ventilation place for six days, soaking the muscles in diluted hydrogen peroxide for four to six days, taking out the muscles, washing out the muscles with clear water and drying the muscles in the air; fixing the muscles onto a glass plate by glue, keeping the muscles in the standing posture of the dog and then placing the muscles into a specimen jar to prepare the whole-body muscle specimen of the adult dog. The method is simple and low in cost, and the prepared muscle specimen of the adult dog is easy to preserve, structurally complete and attractive, and clear and natural in form and position.
Description
Technical field
The present invention relates to a kind of Slide processing, specifically a kind of whole-body muscle Slide processing of adult dogs.
Background technology
Zoological specimens, be to keep former state in kind or process to arrange referential individuality that can be used as representative in one species in the time of can supplying study, research, also can be used as the important references of the universal publicity of scientific research, teaching and Sci-tech Knowledge.The scientific value can not be substituted, ornamental value and reserve value that animal and tissue specimen contain, other artworks of erect image equally enter into family, become the indoor new ornament of people.Make exquisite sample for one and not only bring people's knowledge, also bring the enjoyment of people's art simultaneously.Perfectly the animal muscle sample is the practical teaching aid of an image, can be again the artwork of an exquisiteness, and it represents the development level of colleges and universities, a subject and grasps this subject researcher's professional skill.Preparation of specimen is also a research work, in manufacturing process, can constantly discover problems and solve them, and creates new technology, can broaden one's knowledge, and tempers participant's thinking and manipulative ability simultaneously.Be made into whole muscle specimen, can demonstrate the whole-body muscle structure, the sample structure is attractive in appearance, form is known nature, not only " Zootomy " teaching in as directly perceived, vivid teaching aid, and in manufacturing process, can allow the student participate in, and allow the student understand and grasp preparation of specimen's process, cultivate student's the ability of starting, beat one's brains simultaneously.Contribute to the understanding to animal whole-body muscle overview to the student, the contour structures of whole-body muscle is grasped, the understanding of the Name & Location relation of muscle is all played to good help, contribute to improve teaching efficiency.
Summary of the invention
The present invention seeks to as solving the problems of the technologies described above, a kind of muscle specimen method for making of adult dogs is provided, its preparation method is simple, and prepared sample can demonstrate the whole-body muscle of the dog that grows up, and structure is attractive in appearance, and form is known nature.
A kind of muscle specimen method for making of adult dogs, its concrete preparation process comprises:
Step 1, selection anesthesia, the corpse of the adult dogs of putting to death through perfusion fixation;
Step 2, peeling: the dog corpse is faced upward and is put on autopsy table, with operating scissors, cut at front wrist joint and hind leg gambrel inboard the osculum that diameter is 1-2cm respectively, then cut off dog skin to anus the place ahead from cut osculum inboard respectively; Then separate dog skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation dog: the muscle of isolating the dog whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along boundary, the terminal of muscle, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated dog muscle specimen, water is rinsed well, is placed in the infuse container, then adds the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, add again absolute ethyl alcohol that volume ratio is 1.5:2 and the mixed solution of dimethylbenzene, make absolute ethyl alcohol and dimethylbenzene mixed solution not have dog muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.5:2 in the time of 1-2 days, soak two days later and take out; Perhaps isolated dog muscle is placed in to the infuse container, pours gasoline into, airtight, soak and within about 6-7 days, carry out degreasing.In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 5, degreasing: the dog muscle after degreasing is placed on to dark ventilation and dries in the air 6 days, then the detergent solution that is 5%-7% by mass percent by the dog muscle that dries well soaks 1-2h, then clean three times with clear water;
The preparation method of the detergent solution that described mass percent is 5%-7% is: take washing powder, add distilled water, prepare the detergent solution that mass percent is 5%-7%, matching while using or be placed in 4 ℃ of preservations;
Step 6, the dog muscle after above-mentioned steps five is processed are inserted the hydrogen peroxide after dilution, soak and take out after 4-6 days, with clear water, rinse well, dry;
The preparation method of the hydrogen peroxide after described dilution is: with distilled water, hydrogen peroxide is diluted to the solution that the mass percent of hydrogen peroxide is 4%, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 ℃ of preservations;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep dog to stand, then put in specimen jar, prepares the whole-body muscle sample of adult dogs.
Beneficial effect is:
1, the muscle specimen method for making of adult dogs of the present invention, simple, the easy row of preparation method, with low cost; Adopt ethanol water, absolute ethyl alcohol and dimethylbenzene or gasoline to carry out ungrease treatment to dog muscle, degreasing is thorough, can prevent from using and preserving permanent, and slowly the oozing out of dog muscle grease, pollute mouldyly, makes sample impaired, hinders to use and attractive in appearance.After the dilution that the mass percent that adopts hydrogen oxide is 4%, hydrogen peroxide is bleached, and effect is better and to dog muscle specimen not damaged.
2, the muscle specimen method for making of adult dogs of the present invention, the muscle specimen of prepared dog, be easy to preserve, structural integrity is attractive in appearance, morphologic localization is known nature, and whole-body muscle all clearly illustrated, each piece muscle all represents and be fixed on the physical slot of dog body well.Solved the problem that is difficult to clear resolution dog whole-body muscle.Muscle is the emphasis in Teaching of Anatomy, and the muscle in the Systematic Anatomy teaching is mainly theoretical, and topologic Students in Teaching is not dissected whole muscle; The present invention preferably resolves this difficult problem.Excite again student's learning interest, improved teaching efficiency.
Embodiment
A kind of muscle specimen method for making of adult dogs, its concrete preparation process comprises:
A kind of muscle specimen method for making of adult dogs, its concrete preparation process comprises:
Step 1, selection anesthesia, the corpse of the adult dogs of putting to death through perfusion fixation;
Step 2, peeling: the dog corpse is faced upward and is put on autopsy table, with operating scissors, cut at front wrist joint and hind leg gambrel inboard the osculum that diameter is 1-2cm respectively, then cut off dog skin to anus the place ahead from cut osculum inboard respectively; Then separate dog skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation dog: the muscle of isolating the dog whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along boundary, the terminal of muscle, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated dog muscle specimen, water is rinsed well, is placed in the infuse container, then adds the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, add again absolute ethyl alcohol that volume ratio is 1.5:2 and the mixed solution of dimethylbenzene, make absolute ethyl alcohol and dimethylbenzene mixed solution not have dog muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.5:2 in the time of 1-2 days, soak two days later and take out; Perhaps isolated dog muscle is placed in to the infuse container, pours gasoline into, airtight, soak and within about 6-7 days, carry out degreasing.In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 5, degreasing: the dog muscle after degreasing is placed on to dark ventilation and dries in the air 6 days, then the detergent solution that is 5%-7% by mass percent by the dog muscle that dries well soaks 1-2h, then clean three times with clear water;
The preparation method of the detergent solution that described mass percent is 5%-7% is: take washing powder, add distilled water, prepare the detergent solution that mass percent is 5%-7%, matching while using or be placed in 4 ℃ of preservations;
Step 6, the dog muscle after above-mentioned steps five is processed are inserted the hydrogen peroxide after dilution, soak and take out after 4-6 days, with clear water, rinse well, dry;
The preparation method of the hydrogen peroxide after described dilution is: with distilled water, hydrogen peroxide is diluted to the solution that the mass percent of hydrogen peroxide is 4%, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 ℃ of preservations;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep dog to stand, then put in specimen jar, prepares the whole-body muscle sample of adult dogs.
Following examples can make the present invention of those skilled in the art's complete understanding, but do not limit the present invention in any way.
Embodiment 1
A kind of muscle specimen method for making of adult dogs, its concrete preparation process comprises:
Step 1, select one of plentiful and substantial, the healthy and strong adult dogs of build, claim its body weight;
Step 2, solution preparation: take yellow Jackets according to the body weight of dog, standard is that every 1kg dog takes yellow Jackets 45mg, it is dissolved in to stroke-physiological saline solution and is made into the amobarbital sodium solution that massfraction is 3%, standby; With distilled water, formalin solution is diluted to the solution that the mass percent of formaldehyde is 7%, prepares the formalin solution after dilution, standby;
Step 3, perfusion fixation: draw the prepared amobarbital sodium solution of above-mentioned steps two with syringe, adopt the intraperitoneal injection anesthetized dog; Then opening diameter at the skin place of dog jugular vein ditch with scalpel is the mouth of 3 cm, exposes arteria carotis communis; Cut the osculum that diameter is 0.3cm on arteria carotis communis with operating scissors, insert the perfusion syringe needle in osculum, with hemostatic forceps, fix, quick filling 3000ml physiological saline, treat that blood rinses well, after liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, continue approximately two hours;
Step 2, peeling: the dog corpse is faced upward and is put on autopsy table, with operating scissors, cut at front wrist joint and hind leg gambrel inboard the osculum that diameter is 1cm respectively, then cut off dog skin to anus the place ahead from cut osculum inboard respectively; Then separate dog skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation dog: the muscle of isolating the dog whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along boundary, the terminal of muscle, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated dog muscle specimen, water is rinsed well, is placed in the infuse container, then adds the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, add again absolute ethyl alcohol that volume ratio is 1.5:2 and the mixed solution of dimethylbenzene, make absolute ethyl alcohol and dimethylbenzene mixed solution not have dog muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.5:2 in the time of 1 day, soak two days later and take out; In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 5, degreasing: the dog muscle after degreasing is placed on to dark ventilation and dries in the air 6 days, then the detergent solution that is 5% by mass percent by the dog muscle that dries well soaks 1h, then clean three times with clear water;
The preparation method of the detergent solution that described mass percent is 5% is: take washing powder, add distilled water, prepare the detergent solution that mass percent is 5%, matching while using or be placed in 4 ℃ of preservations;
Step 6, the dog muscle after above-mentioned steps five is processed are inserted the hydrogen peroxide after dilution, soak and take out after 4-6 days, with clear water, rinse well, dry;
The preparation method of the hydrogen peroxide after described dilution is: with distilled water, hydrogen peroxide is diluted to the solution that the mass percent of hydrogen peroxide is 4%, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 ℃ of preservations;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep dog to stand, then put in specimen jar, prepares the whole-body muscle sample of adult dogs.
Embodiment 2
Step 1, select one of plentiful and substantial, the healthy and strong adult dogs of build, claim its body weight;
Step 2, solution preparation: take yellow Jackets according to the body weight of dog, standard is that every 1kg dog takes yellow Jackets 45mg, it is dissolved in to stroke-physiological saline solution and is made into the amobarbital sodium solution that massfraction is 3%, standby; With distilled water, formalin solution is diluted to the solution that the mass percent of formaldehyde is 7%, prepares the formalin solution after dilution, standby;
Step 3, perfusion fixation: draw the prepared amobarbital sodium solution of above-mentioned steps two with syringe, adopt the intraperitoneal injection anesthetized dog; Then opening diameter at the skin place of dog jugular vein ditch with scalpel is the mouth of 3 cm, exposes arteria carotis communis; Cut the osculum that diameter is 0.3cm on arteria carotis communis with operating scissors, insert the perfusion syringe needle in osculum, with hemostatic forceps, fix, quick filling 3000ml physiological saline, treat that blood rinses well, after liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, continue approximately two hours;
Step 2, peeling: the dog corpse is faced upward and is put on autopsy table, with operating scissors, cut at front wrist joint and hind leg gambrel inboard the osculum that diameter is 2cm respectively, then cut off dog skin to anus the place ahead from cut osculum inboard respectively; Then separate dog skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation dog: the muscle of isolating the dog whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along boundary, the terminal of muscle, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated dog muscle specimen, water is rinsed well, is placed in the infuse container, then adds the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, add again absolute ethyl alcohol that volume ratio is 1.5:2 and the mixed solution of dimethylbenzene, make absolute ethyl alcohol and dimethylbenzene mixed solution not have dog muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.5:2 in the time of 2 days, soak two days later and take out; In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 5, degreasing: the dog muscle after degreasing is placed on to dark ventilation and dries in the air 6 days, then the detergent solution that is 7% by mass percent by the dog muscle that dries well soaks 2h, then clean three times with clear water;
The preparation method of the detergent solution that described mass percent is 7% is: take washing powder, add distilled water, prepare the detergent solution that mass percent is 7%, matching while using or be placed in 4 ℃ of preservations;
Step 6, the dog muscle after above-mentioned steps five is processed are inserted the hydrogen peroxide after dilution, soak and take out afterwards in 6 days, with clear water, rinse well, dry;
The preparation method of the hydrogen peroxide after described dilution is: with distilled water, hydrogen peroxide is diluted to the solution that the mass percent of hydrogen peroxide is 4%, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 ℃ of preservations;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep dog to stand, then put in specimen jar, prepares the whole-body muscle sample of adult dogs.
Embodiment 3
Step 1, select one of plentiful and substantial, the healthy and strong adult dogs of build, claim its body weight;
Step 2, solution preparation: take yellow Jackets according to the body weight of dog, standard is that every 1kg dog takes yellow Jackets 45mg, it is dissolved in to stroke-physiological saline solution and is made into the amobarbital sodium solution that massfraction is 3%, standby; With distilled water, formalin solution is diluted to the solution that the mass percent of formaldehyde is 7%, prepares the formalin solution after dilution, standby;
Step 3, perfusion fixation: draw the prepared amobarbital sodium solution of above-mentioned steps two with syringe, adopt the intraperitoneal injection anesthetized dog; Then opening diameter at the skin place of dog jugular vein ditch with scalpel is the mouth of 3 cm, exposes arteria carotis communis; Cut the osculum that diameter is 0.3cm on arteria carotis communis with operating scissors, insert the perfusion syringe needle in osculum, with hemostatic forceps, fix, quick filling 3000ml physiological saline, treat that blood rinses well, after liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, continue approximately two hours;
Step 2, peeling: the dog corpse is faced upward and is put on autopsy table, with operating scissors, cut at front wrist joint and hind leg gambrel inboard the osculum that diameter is 1.5cm respectively, then cut off dog skin to anus the place ahead from cut osculum inboard respectively; Then separate dog skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation dog: the muscle of isolating the dog whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along boundary, the terminal of muscle, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated dog muscle specimen, water is rinsed well, is placed in the infuse container, then adds the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, add again absolute ethyl alcohol that volume ratio is 1.5:2 and the mixed solution of dimethylbenzene, make absolute ethyl alcohol and dimethylbenzene mixed solution not have dog muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.5:2 in the time of 1 day, soak two days later and take out; In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 5, degreasing: the dog muscle after degreasing is placed on to dark ventilation and dries in the air 6 days, then the detergent solution that is 6% by mass percent by the dog muscle that dries well soaks 1.5h, then clean three times with clear water;
The preparation method of the detergent solution that described mass percent is 6% is: take washing powder, add distilled water, prepare the detergent solution that mass percent is 6%, matching while using or be placed in 4 ℃ of preservations;
Step 6, the dog muscle after above-mentioned steps five is processed are inserted the hydrogen peroxide after dilution, soak and take out after 4-6 days, with clear water, rinse well, dry;
The preparation method of the hydrogen peroxide after described dilution is: with distilled water, hydrogen peroxide is diluted to the solution that the mass percent of hydrogen peroxide is 4%, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 ℃ of preservations;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep dog to stand, then put in specimen jar, prepares the whole-body muscle sample of adult dogs.
Embodiment 4
Step 1, select one of plentiful and substantial, the healthy and strong adult dogs of build, claim its body weight;
Step 2, solution preparation: take yellow Jackets according to the body weight of dog, standard is that every 1kg dog takes yellow Jackets 45mg, it is dissolved in to stroke-physiological saline solution and is made into the amobarbital sodium solution that massfraction is 3%, standby; With distilled water, formalin solution is diluted to the solution that the mass percent of formaldehyde is 7%, prepares the formalin solution after dilution, standby;
Step 3, perfusion fixation: draw the prepared amobarbital sodium solution of above-mentioned steps two with syringe, adopt the intraperitoneal injection anesthetized dog; Then opening diameter at the skin place of dog jugular vein ditch with scalpel is the mouth of 3 cm, exposes arteria carotis communis; Cut the osculum that diameter is 0.3cm on arteria carotis communis with operating scissors, insert the perfusion syringe needle in osculum, with hemostatic forceps, fix, quick filling 3000ml physiological saline, treat that blood rinses well, after liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, continue approximately two hours;
Step 2, peeling: the dog corpse is faced upward and is put on autopsy table, with operating scissors, cut at front wrist joint and hind leg gambrel inboard the osculum that diameter is 1.3cm respectively, then cut off dog skin to anus the place ahead from cut osculum inboard respectively; Then separate dog skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation dog: the muscle of isolating the dog whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along boundary, the terminal of muscle, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated dog muscle specimen, water is rinsed well, is placed in the infuse container, pours gasoline into, airtight, soaks and approximately within 7 days, carries out degreasing.In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 5, degreasing: the dog muscle after degreasing is placed on to dark ventilation and dries in the air 6 days, then the detergent solution that is 7% by mass percent by the dog muscle that dries well soaks 2h, then clean three times with clear water;
The preparation method of the detergent solution that described mass percent is 5.5% is: take washing powder, add distilled water, prepare the detergent solution that mass percent is 5.5%, matching while using or be placed in 4 ℃ of preservations;
Step 6, the dog muscle after above-mentioned steps five is processed are inserted the hydrogen peroxide after dilution, soak and take out afterwards in 5 days, with clear water, rinse well, dry;
The preparation method of the hydrogen peroxide after described dilution is: with distilled water, hydrogen peroxide is diluted to the solution that the mass percent of hydrogen peroxide is 4%, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 ℃ of preservations;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep dog to stand, then put in specimen jar, prepares the whole-body muscle sample of adult dogs.
Claims (3)
1. the whole-body muscle Slide processing of an adult dogs, it is characterized in that: its concrete preparation process comprises:
Step 1, selection anesthesia, the corpse of the adult dogs of putting to death through perfusion fixation;
Step 2, peeling: the dog corpse is faced upward and is put on autopsy table, with operating scissors, cut at front wrist joint and hind leg gambrel inboard the osculum that diameter is 1-2cm respectively, then cut off dog skin to anus the place ahead from cut osculum inboard respectively; Then separate dog skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation dog: the muscle of isolating the dog whole body with scalpel;
Step 4, degreasing: by isolated dog muscle specimen, water is rinsed well, is placed in the infuse container, then adds the ethanol water that mass percent is 88%, makes ethanol water not have dog muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, add again absolute ethyl alcohol that volume ratio is 1.5:2 and the mixed solution of dimethylbenzene, make absolute ethyl alcohol and dimethylbenzene mixed solution not have dog muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.5:2 in the time of 1-2 days, soak two days later and take out;
Processing after step 5, degreasing: the dog muscle after degreasing is placed on to dark ventilation and dries in the air 6 days, then the detergent solution that is 5%-7% by mass percent by the dog muscle that dries well soaks 1-2h, then clean three times with clear water;
The preparation method of the detergent solution that described mass percent is 5%-7% is: take washing powder, add distilled water, prepare the detergent solution that mass percent is 5%-7%, matching while using or be placed in 4 ℃ of preservations;
Step 6, the dog muscle after above-mentioned steps five is processed are inserted the hydrogen peroxide after dilution, soak and take out after 4-6 days, with clear water, rinse well, dry;
The preparation method of the hydrogen peroxide after described dilution is: with distilled water, hydrogen peroxide is diluted to the solution that the mass percent of hydrogen peroxide is 4%, prepares the hydrogen peroxide after dilution, matching while using or be placed in 4 ℃ of preservations;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep dog to stand, then put in specimen jar, prepares the whole-body muscle sample of adult dogs.
2. the whole-body muscle Slide processing of a kind of adult dogs as claimed in claim 1 is characterized in that: in the operating process of described step 5 degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
3. the whole-body muscle Slide processing of a kind of adult dogs as claimed in claim 1, it is characterized in that: the degreasing method in described step 5 also can adopt: isolated dog muscle specimen is placed in to the infuse container, pour gasoline into, airtight, soak and within 5 days, carry out degreasing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310361338.5A CN103426354B (en) | 2013-08-19 | 2013-08-19 | A kind of whole-body muscle Slide processing of adult dogs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310361338.5A CN103426354B (en) | 2013-08-19 | 2013-08-19 | A kind of whole-body muscle Slide processing of adult dogs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103426354A true CN103426354A (en) | 2013-12-04 |
| CN103426354B CN103426354B (en) | 2016-08-10 |
Family
ID=49651025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310361338.5A Expired - Fee Related CN103426354B (en) | 2013-08-19 | 2013-08-19 | A kind of whole-body muscle Slide processing of adult dogs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103426354B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103824499A (en) * | 2014-01-29 | 2014-05-28 | 河南科技大学 | Manufacturing method of adult rabbit specimen |
| CN103814889A (en) * | 2014-01-29 | 2014-05-28 | 河南科技大学 | Method for making adult male dog specimen |
| CN105280071A (en) * | 2015-12-03 | 2016-01-27 | 河南科技大学 | Method for manufacturing pig ear intravenous injection model based on mathematical modeling |
| CN105355127A (en) * | 2015-12-03 | 2016-02-24 | 河南科技大学 | Mathematical modeling-based adult rat tail vein injection model fabricating method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1835046A (en) * | 2005-03-18 | 2006-09-20 | 中国科学院海洋研究所 | Prodn. method of large ocean mammal and fishes samples with high fat content |
| CN1971663A (en) * | 2006-12-06 | 2007-05-30 | 王荣林 | Dry type manufacturing method of animal specimen |
| CN101034508A (en) * | 2007-03-27 | 2007-09-12 | 中国水产科学研究院南海水产研究所 | Method for making dolphin skinned-specimen |
| CN102499231A (en) * | 2011-10-21 | 2012-06-20 | 水利部中国科学院水工程生态研究所 | Harmless fabrication method of sturgeon taxidermy specimen |
| CN102945638A (en) * | 2012-11-01 | 2013-02-27 | 哈尔滨师范大学 | Method for preparing cowfish stuffed specimen |
-
2013
- 2013-08-19 CN CN201310361338.5A patent/CN103426354B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1835046A (en) * | 2005-03-18 | 2006-09-20 | 中国科学院海洋研究所 | Prodn. method of large ocean mammal and fishes samples with high fat content |
| CN1971663A (en) * | 2006-12-06 | 2007-05-30 | 王荣林 | Dry type manufacturing method of animal specimen |
| CN101034508A (en) * | 2007-03-27 | 2007-09-12 | 中国水产科学研究院南海水产研究所 | Method for making dolphin skinned-specimen |
| CN102499231A (en) * | 2011-10-21 | 2012-06-20 | 水利部中国科学院水工程生态研究所 | Harmless fabrication method of sturgeon taxidermy specimen |
| CN102945638A (en) * | 2012-11-01 | 2013-02-27 | 哈尔滨师范大学 | Method for preparing cowfish stuffed specimen |
Non-Patent Citations (2)
| Title |
|---|
| 李仲购 等: "《介绍一种神经系统全貌标本制作技术》", 《中国临床解剖学杂志》, vol. 25, no. 3, 31 December 2007 (2007-12-31), pages 3 * |
| 李清滨: "《肌肉石蜡干燥标本制作方法》", 《解剖学杂志》, no. 1, 2 March 1986 (1986-03-02), pages 41 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103824499A (en) * | 2014-01-29 | 2014-05-28 | 河南科技大学 | Manufacturing method of adult rabbit specimen |
| CN103814889A (en) * | 2014-01-29 | 2014-05-28 | 河南科技大学 | Method for making adult male dog specimen |
| CN103814889B (en) * | 2014-01-29 | 2015-10-28 | 河南科技大学 | A kind of preparation method of adult public dog sample |
| CN103824499B (en) * | 2014-01-29 | 2016-05-11 | 河南科技大学 | A kind of preparation method of adult rabbits sample |
| CN105280071A (en) * | 2015-12-03 | 2016-01-27 | 河南科技大学 | Method for manufacturing pig ear intravenous injection model based on mathematical modeling |
| CN105355127A (en) * | 2015-12-03 | 2016-02-24 | 河南科技大学 | Mathematical modeling-based adult rat tail vein injection model fabricating method |
| CN105355127B (en) * | 2015-12-03 | 2017-10-17 | 河南科技大学 | A kind of adult rat tail vein injection model production method based on mathematical modeling |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103426354B (en) | 2016-08-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102499231B (en) | Harmless fabrication method of sturgeon taxidermy specimen | |
| CN103426354A (en) | Method for preparing whole-body muscle specimen of adult dog | |
| CN103430934B (en) | Manufacturing method of adult cat musclespecimen | |
| Vogel | Prime mover: a natural history of muscle | |
| CN102945638B (en) | The manufacture method of Neomeris phocaenoides G. Cuvier skinned-specimen | |
| CN103400529B (en) | A kind of whole-body muscle Slide processing of mature rooster | |
| CN103380768B (en) | Manufacturing method of adult ram nervous system specimen | |
| CN103404506B (en) | Method for preparing specimen of muscle of whole adult fox | |
| CN103824499B (en) | A kind of preparation method of adult rabbits sample | |
| CN103426355B (en) | The whole-body muscle Slide processing of a kind of adult otter | |
| CN103824497B (en) | A kind of preparation method of adult dog fox sample | |
| Müller et al. | Traditional and modern biomedical prospecting: Part I—the history | |
| CN103500526A (en) | Method for preparing muscle specimen of adult rabbit | |
| CN107568198A (en) | The sample for preparing the method for meiofauna pattern of glassy carcass and preparing in this way | |
| CN103814888B (en) | Method for making adult cat specimen | |
| CN103824496B (en) | A kind of preparation method of adult ram sample | |
| CN103824494B (en) | A kind of preparation method of mature rooster sample | |
| CN105355127B (en) | A kind of adult rat tail vein injection model production method based on mathematical modeling | |
| CN103824495B (en) | A kind of preparation method of adult otter sample | |
| CN103824498B (en) | A kind of preparation method of adult duck sample | |
| Spicer | Ontogeny of cardiac function in the brine shrimp Artemia franciscana Kellogg 1906 (Branchiopoda: Anostraca) | |
| CN106417249B (en) | Preparation method of crab aquatic animal stripping specimen | |
| CN105280071B (en) | A kind of pig ear vein injection model preparation method based on mathematical modeling | |
| CN103814889B (en) | A kind of preparation method of adult public dog sample | |
| CN102367433A (en) | Suspension culture method for intestinal cells of stomachless lukewarm water fish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160810 Termination date: 20170819 |