CN105280071B - A kind of pig ear vein injection model preparation method based on mathematical modeling - Google Patents
A kind of pig ear vein injection model preparation method based on mathematical modeling Download PDFInfo
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Abstract
A kind of pig ear vein injection model preparation method based on mathematical modeling, configuration, the separation of tail vein of medicament are carried out first, then configured medicament is utilized to make pig lug die and ear vein silicone rubber model respectively, then ear vein silicone rubber model is embedded in the ear vein ditch in pig lug die, and wraps up skin model and can be made into pig ear vein injection model.The inventive method is simple, it is with low cost, produced ear vein injection model is easy to preserve, and structural integrity, attractive in appearance, morphologic localization understands nature, it can be used for repeatability exercise, the deficiency that the injection of live hog ear vein can not be repeated the operation several times is made up, it is difficult the problem of accomplishing that penetratingly ear vein can not be injected repeatedly with live hog, both to have shortened the teachers' instruction time to solve beginner's injection, the learning interest of student is excited again, improves teaching efficiency.
Description
Technical field
The present invention relates to a kind of preparation method of test animal model, specifically a kind of pig based on mathematical modeling
Ear vein injection model preparation method.
Background technology
Animal model, is to maintain referential same when animal body structure is available for learning, studied as former state or by arrangement
Can be as the individual represented in one species, the important references that can be also publicized as the popularization of scientific research, teaching and Sci-tech Knowledge.
Animal and tissue model contain the scientific value that can not be substituted, ornamental value and reserve value, and other arts work of erect image are equally walked
Enter market, exhibition center and family, as ornament new in people room.Not only band gives people to know the exquisite model of one making
Know, while going back the enjoyment that band gives people art.Perfect animal model is both a vivid practical teaching aid, can be one again
The consummate art work, it represents a colleges and universities, the development level of subject and the professional skill for grasping disciplinary study person.It is dynamic
Thing modelling is also a research work, in manufacturing process, can constantly be discovered problems and solve them, and creates new technology,
It can broaden one's knowledge simultaneously, temper thinking and the manipulative ability of participant.
The mesofaunas such as pig, rabbit, sheep inject the methods injected more using ear vein with transfusion, still, and beginner is in study
When majority can not smoothly complete operation, often result in animal ear hemotoncus, moreover, can not simultaneously many people repeatedly operate repeatedly.
Ear vein injection is the emphasis and difficult point part of animal administration in animal medicine practical teaching, Systematic Anatomy teaching
In practice to be mainly theoretical many practical operations few, moreover, the Students in Teaching of Local Anatomy can only typically see books
On lines figure, and be not aware that morphosis, color and luster, the size etc. of specific organ and tissue.
Animal ear vein injection model is made, animal ear vein can be appeared clearly from, the model structure is attractive in appearance, and form is clear
Chu naturally, simultaneously serve intravenous injection operation teaching and exercise well.Ear vein injection model is not only in " zootomy tissue
As the teaching aid of image directly perceived in " teaching, and student can be allowed to participate in manufacturing process, student is understood model system
Make process, and grasp this technology, while starting, beating one's brains and operational capacity for training student, contributes to repeatability to practise, make up
The injection of live hog ear vein can not the multiple deficiency of injection operation repeatedly, beneficial to improving teaching efficiency.
The content of the invention
It is an object of the present invention to provide a kind of pig ear vein injection model preparation method based on mathematical modeling, its preparation method
Simply, produced ear vein injection model can clearly indicate out ear vein, and structure is attractive in appearance, and form is understood naturally, being beneficial to
The operation repeatedly that beginner injects to ear vein in experiment.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：A kind of pig ear based on mathematical modeling
Model production method is injected intravenously, is comprised the following steps：
Step 1: the preparation of medicament：Picric acid saturated solution 60ml ~ 70ml is taken, 4g ~ 6g paraformaldehydes, 5ml are added thereto
~ 7ml glacial acetic acid and 30ml ~ 40ml distilled water, are stirred and evenly mixed, and fixer is made, standby；According to volume ratio, by 10% ~ 15%
Emulsifying wax liquid, 15% ~ 20% methyl-silicone oil emulsion, 52% ~ 60% deionized water, 10% ~ 15% polyacrylamide amine emulsifier and
0.2% preservative is well mixed to be made releasing agent, standby；According to mass ratio, take 1% ~ 2% NSJF-3B rubber powders, 44% ~ 60%
Powdered whiting, 20% ~ 25% sierozem power and 18% ~ 30% white cement, add water stir thereto, and gypsum breast is made, standby
With；Take molded silicon rubber and the silicon rubber of blueness is made in addition RTV-2 crosslinking agents thereto, it is standby；
Step 2: after selection anesthesia, the corpse for the pig that the fixer perfusion fixation prepared with step one is put to death；
Step 3: pig corpse is lain on one's side, a diameter of 0.5 ~ 1 cm osculum is cut in the ear portion median line respectively with operating scissors,
Then cut forward to have sharp ears from cut osculum respectively；Then pig ear skin is separated, is sloughed skin to have sharp ears with the method for drill drift,
The housing type of dermatotome outwardly is become, then cuts the auricular cartilage of ear, skin is completely stripped；
Step 4: isolating ear vein in the ear after step 3 peeling, and clean out, then successively in ear
Releasing agent and the gypsum breast that squeegeeing step one is prepared, are made pig lug die；
Step 5: inserting support tube in the ear vein that step 4 is separated, blood vessel character is propped up, then in ear vein
Releasing agent and silicon rubber that upper priority squeegeeing step one is prepared, are made ear vein silicone rubber model, and in described branch
Founding mathematical models are as follows between the internal diameter of stay tube, external diameter and ear vein length：
y1=-0.01x+0.3；
y2=-0.01x+0.4；
Wherein, x represents ear vein length, y1Represent the internal diameter of support tube, y2Represent the external diameter of support tube；
Step 6: after the silicon rubber in ear vein silicone rubber model solidifies, by ear vein silicone rubber model Embedded step
In the ear vein ditch in pig lug die made by four, then brushing silica gel, and uniform on silica gel before silica gel dries
Pig hair is stained with, to form the skin model being wrapped on pig lug die, is positioned in specimen jar, that is, the injection of pig ear vein is made
Model.
Further, the medicament prepared in step one is matching while using or 4 DEG C of conditions is placed in after medicament configuration
It is lower to preserve.
Further, in step 4,5 times gypsum breasts are brushed after brushing releasing agent by ear.
Further, in step one, the addition of RTV-2 crosslinking agents is the 1% ~ 2% of molded silicon rubber quality.
Further, in step one, preservative used is o-phenyl phenol preservative.
The beneficial effects of the invention are as follows：
1st, the inventive method is simple, ear vein injection model with low cost, produced, it is easy to preserve, structural integrity
Attractive in appearance, morphologic localization, which is understood naturally, can be used for repeatability, to be practised, and makes up what the injection of live hog ear vein can not be repeated the operation several times
Deficiency, so that it is difficult the problem of accomplishing that penetratingly ear vein can not be injected repeatedly with live hog, both to contract to solve beginner's injection
The short teachers' instruction time, the learning interest of student is excited again, teaching efficiency is improved.
2nd, the pig ear vein injection model made by the present invention can appear clearly from ear vein, and the model structure is attractive in appearance,
Form is understood naturally, and serving intravenous injection operation teaching well with practising；Ear vein injection model is not only in " animal solution
Cut open histology " in teaching as teaching aid directly perceived, vivid, and student can be allowed to participate in manufacturing process, understand student
Modelling process, and grasp this technology, while training student is started, beaten one's brains and operational capacity.
3rd, the present invention is irrigated using special fixer, and it is formulated and the size of concentration is by substantial amounts of experimental summary
, effect is preferable in the making of ear vein injection model；Using emulsifying wax liquid, methyl-silicone oil emulsion, deionized water, polypropylene
Acid amides emulsifying agent and o-phenyl phenol preservative prepare releasing agent, and stripping result is preferable；Condensation polymer type is added in molded silicon rubber
Two-component silicone rubber（RTV-2）Crosslinking agent, is made the silicon rubber of blueness, makes ear vein injection model, nontoxic, tasteless, stingless
Swash, convenient use, be easy to preserve.
Embodiment
The present invention is further explained below by specific embodiment.
Embodiment 1：A kind of pig ear vein injection model preparation method based on mathematical modeling, its specific make step is：
Step 1: preparing fixer：Measure the ml of picric acid saturated solution 70, paraformaldehyde 4 g, the ml of distilled water 30 and ice vinegar
5 ml of acid, which are mixed evenly, is made fixer, wherein, the preparation method of picric acid saturated solution is：By 1.5 g picric acid solids
It is dissolved in 100 ml distilled water, mixing；
Step 2: preparing releasing agent：According to volume fraction measure 15% emulsifying wax liquid, 20% methyl-silicone oil emulsion,
54.8% deionized water, 10% polyacrylamide amine emulsifier and 0.2% o-phenyl phenol preservative are well mixed to be can be prepared by
Releasing agent；
Step 3: preparing gypsum breast：According to mass ratio measure 1% NSJF-3B rubber powders, 44% powdered whiting, 25%
Sierozem power and 30% white cement, are dissolved in water and are well mixed, that is, gypsum breast is made；
Step 4: prepared by silicon rubber：According to mass ratio, the condensation polymer type two-component silicone rubber of addition 1% into molded silicon rubber
（That is RTV-2）Crosslinking agent, is made the silicon rubber of blueness；
Step 5: after selection anesthesia, the corpse for the pig that the fixer perfusion fixation prepared with step one is put to death；
Step 6: peeling：Pig corpse is lain on one's side, the small of a diameter of 0.5 cm is cut in the ear portion median line respectively with operating scissors
Mouthful, then cut forward to have sharp ears from cut osculum respectively；Then pig ear skin is separated, is taken off skin to have sharp ears with the method for drill drift
Go, become the housing type of dermatotome outwardly, then cut the auricular cartilage of ear, skin is completely stripped；
Step 7: the making of pig lug die：Isolate ear vein in the ear after step 6 peeling, and by ear
Clean out, pig is made in the gypsum breast that the releasing agent and step 3 then successively prepared in ear's squeegeeing step two are prepared
Lug die, and gypsum breast will brush 5 times, to protrude ear vein ditch therein；
Step 8: the making of ear vein silicone rubber model：Support tube is inserted in the ear vein that step 7 is separated, is supportted
Play blood vessel character, the silicon rubber that the releasing agent and step 4 that then priority squeegeeing step two is prepared on ear vein are prepared
Glue, is made between ear vein silicone rubber model, and the internal diameter in described support tube, external diameter and ear vein length and sets up mathematics
Model is as follows：
y1=-0.01x+0.3；
y2=-0.01x+0.4；
Wherein, x represents ear vein length, y1Represent the internal diameter of support tube, y2Represent the external diameter of support tube；
Step 9: after the silicon rubber in ear vein silicone rubber model solidifies, by ear vein silicone rubber model Embedded step
In the ear vein ditch in pig lug die made by four, then brushing silica gel, and uniform on silica gel before silica gel dries
Pig hair is stained with, to form the skin model being wrapped on pig lug die, is positioned in specimen jar, that is, the injection of pig ear vein is made
Model.
Embodiment 2：A kind of pig ear vein injection model preparation method based on mathematical modeling, its specific make step is：
Step 1: preparing fixer：Measure picric acid saturated solution 60 ml, paraformaldehyde 6g, the ml of distilled water 40 and ice vinegar
7 ml of acid, which are mixed evenly, is made fixer, wherein, the preparation method of picric acid saturated solution is：By 1.5 g picric acid solids
It is dissolved in 100 ml distilled water, mixing；
Step 2: preparing releasing agent：According to volume fraction measure 10% emulsifying wax liquid, 15% methyl-silicone oil emulsion,
59.8% deionized water, 15% polyacrylamide amine emulsifier and 0.2% o-phenyl phenol preservative are well mixed to be can be prepared by
Releasing agent；
Step 3: preparing gypsum breast：According to mass ratio measure 2% NSJF-3B rubber powders, 60% powdered whiting, 20%
Sierozem power and 18% white cement, are dissolved in water and are well mixed, that is, gypsum breast is made；
Step 4: prepared by silicon rubber：According to mass ratio, the condensation polymer type two-component silicone rubber of addition 2% into molded silicon rubber
（That is RTV-2）Crosslinking agent, is made the silicon rubber of blueness；
Step 5: after selection anesthesia, the corpse for the pig that the fixer perfusion fixation prepared with step one is put to death；
Step 6: peeling：Pig corpse is lain on one's side, the small of a diameter of 0.5 cm is cut in the ear portion median line respectively with operating scissors
Mouthful, then cut forward to have sharp ears from cut osculum respectively；Then pig ear skin is separated, is taken off skin to have sharp ears with the method for drill drift
Go, become the housing type of dermatotome outwardly, then cut the auricular cartilage of ear, skin is completely stripped；
Step 7: the making of pig lug die：Isolate ear vein in the ear after step 6 peeling, and by ear
Clean out, pig is made in the gypsum breast that the releasing agent and step 3 then successively prepared in ear's squeegeeing step two are prepared
Lug die, and gypsum breast will brush 5 times, to protrude ear vein ditch therein；
Step 8: the making of ear vein silicone rubber model：Support tube is inserted in the ear vein that step 7 is separated, is supportted
Play blood vessel character, the silicon rubber that the releasing agent and step 4 that then priority squeegeeing step two is prepared on ear vein are prepared
Glue, is made between ear vein silicone rubber model, and the internal diameter in described support tube, external diameter and ear vein length and sets up mathematics
Model is as follows：
y1=-0.01x+0.3；
y2=-0.01x+0.4；
Wherein, x represents ear vein length, y1Represent the internal diameter of support tube, y2Represent the external diameter of support tube；
Step 9: after the silicon rubber in ear vein silicone rubber model solidifies, by ear vein silicone rubber model Embedded step
In the ear vein ditch in pig lug die made by four, then brushing silica gel, and uniform on silica gel before silica gel dries
Pig hair is stained with, to form the skin model being wrapped on pig lug die, is positioned in specimen jar, that is, the injection of pig ear vein is made
Model.
Embodiment 3：A kind of pig ear vein injection model preparation method based on mathematical modeling, its specific make step is：
Step 1: preparing fixer：Measure picric acid saturated solution 65ml, paraformaldehyde 5g, the ml of distilled water 35 and glacial acetic acid
6 ml, which are mixed evenly, is made fixer, wherein, the preparation method of picric acid saturated solution is：1.5 g picric acid solids are molten
Solution is in 100 ml distilled water, mixing；
Step 2: preparing releasing agent：According to volume fraction measure 12% emulsifying wax liquid, 17.8% methyl-silicone oil emulsion,
60% deionized water, 10% polyacrylamide amine emulsifier and 0.2% o-phenyl phenol preservative are well mixed to be can be prepared by taking off
Mould agent；
Step 3: preparing gypsum breast：According to mass ratio measure 1% NSJF-3B rubber powders, 52.5% powdered whiting,
22.5% sierozem power and 24% white cement, are dissolved in water and are well mixed, that is, gypsum breast is made；
Step 4: prepared by silicon rubber：According to mass ratio, the condensation polymer type two-component silicone rubber of addition 1% into molded silicon rubber
（That is RTV-2）Crosslinking agent, is made the silicon rubber of blueness；
Step 5: after selection anesthesia, the corpse for the pig that the fixer perfusion fixation prepared with step one is put to death；
Step 6: peeling：Pig corpse is lain on one's side, the small of a diameter of 1 cm is cut in the ear portion median line respectively with operating scissors
Mouthful, then cut forward to have sharp ears from cut osculum respectively；Then pig ear skin is separated, is taken off skin to have sharp ears with the method for drill drift
Go, become the housing type of dermatotome outwardly, then cut the auricular cartilage of ear, skin is completely stripped；
Step 7: the making of pig lug die：Isolate ear vein in the ear after step 6 peeling, and by ear
Clean out, pig is made in the gypsum breast that the releasing agent and step 3 then successively prepared in ear's squeegeeing step two are prepared
Lug die, and gypsum breast will brush 5 times, to protrude ear vein ditch therein；
Step 8: the making of ear vein silicone rubber model：Support tube is inserted in the ear vein that step 7 is separated, is supportted
Play blood vessel character, the silicon rubber that the releasing agent and step 4 that then priority squeegeeing step two is prepared on ear vein are prepared
Glue, is made between ear vein silicone rubber model, and the internal diameter in described support tube, external diameter and ear vein length and sets up mathematics
Model is as follows：
y1=-0.01x+0.3；
y2=-0.01x+0.4；
Wherein, x represents ear vein length, y1Represent the internal diameter of support tube, y2Represent the external diameter of support tube；
Step 9: after the silicon rubber in ear vein silicone rubber model solidifies, by ear vein silicone rubber model Embedded step
In the ear vein ditch in pig lug die made by four, then brushing silica gel, and uniform on silica gel before silica gel dries
Pig hair is stained with, to form the skin model being wrapped on pig lug die, is positioned in specimen jar, that is, the injection of pig ear vein is made
Model.
Embodiment 4, a kind of pig ear vein injection model preparation method based on mathematical modeling, its specific make step is：
Step 1: preparing fixer：Measure picric acid saturated solution 60ml, paraformaldehyde 5g, the ml of distilled water 30 and glacial acetic acid
7 ml, which are mixed evenly, is made fixer, wherein, the preparation method of picric acid saturated solution is：1.5 g picric acid solids are molten
Solution is in 100 ml distilled water, mixing；
Step 2: preparing releasing agent：According to volume fraction measure 15% emulsifying wax liquid, 20% methyl-silicone oil emulsion, 52%
Deionized water, 12.8% polyacrylamide amine emulsifier and 0.2% o-phenyl phenol preservative are well mixed can be prepared by taking off
Mould agent；
Step 3: preparing gypsum breast：According to mass ratio measure 1.5% NSJF-3B rubber powders, 55.5% powdered whiting,
20% sierozem power and 23% white cement, are dissolved in water and are well mixed, that is, gypsum breast is made；
Step 4: prepared by silicon rubber：According to mass ratio, the condensation polymer type two-component silicon rubber of addition 1.5% into molded silicon rubber
Glue（That is RTV-2）Crosslinking agent, is made the silicon rubber of blueness；
Step 5: after selection anesthesia, the corpse for the pig that the fixer perfusion fixation prepared with step one is put to death；
Step 6: peeling：Pig corpse is lain on one's side, the small of a diameter of 1 cm is cut in the ear portion median line respectively with operating scissors
Mouthful, then cut forward to have sharp ears from cut osculum respectively；Then pig ear skin is separated, is taken off skin to have sharp ears with the method for drill drift
Go, become the housing type of dermatotome outwardly, then cut the auricular cartilage of ear, skin is completely stripped；
Step 7: the making of pig lug die：Isolate ear vein in the ear after step 6 peeling, and by ear
Clean out, pig is made in the gypsum breast that the releasing agent and step 3 then successively prepared in ear's squeegeeing step two are prepared
Lug die, and gypsum breast will brush 5 times, to protrude ear vein ditch therein；
Step 8: the making of ear vein silicone rubber model：Support tube is inserted in the ear vein that step 7 is separated, is supportted
Play blood vessel character, the silicon rubber that the releasing agent and step 4 that then priority squeegeeing step two is prepared on ear vein are prepared
Glue, is made between ear vein silicone rubber model, and the internal diameter in described support tube, external diameter and ear vein length and sets up mathematics
Model is as follows：
y1=-0.01x+0.3；
y2=-0.01x+0.4；
Wherein, x represents ear vein length, y1Represent the internal diameter of support tube, y2Represent the external diameter of support tube；
Step 9: after the silicon rubber in ear vein silicone rubber model solidifies, by ear vein silicone rubber model Embedded step
In the ear vein ditch in pig lug die made by four, then brushing silica gel, and uniform on silica gel before silica gel dries
Pig hair is stained with, to form the skin model being wrapped on pig lug die, is positioned in specimen jar, that is, the injection of pig ear vein is made
Model.
In the specific step of the various embodiments described above, involved medicament（Fixer, releasing agent, gypsum breast and silicon
Rubber）Preferably with the principle of matching while using, or prepare and be stored in 4 DEG C of environment.
Support tube in the step 8 can select plastic tube.
Support bore, mathematical modeling between external diameter and ear vein length is exemplified below, as the long 10cm of ear vein,
The internal diameter of required support tube is 0.2cm, and external diameter is 0.3cm.
Claims (5)
1. a kind of pig ear vein injection model preparation method based on mathematical modeling, it is characterised in that：Comprise the following steps：
Step 1: the preparation of medicament：Picric acid saturated solution 60ml ~ 70ml is taken, 4g ~ 6g paraformaldehydes, 5ml ~ 7ml are added thereto
Glacial acetic acid and 30ml ~ 40ml distilled water, are stirred and evenly mixed, and fixer is made, standby；According to volume ratio, by 10% ~ 15% emulsification
Wax liquor, 15% ~ 20% methyl-silicone oil emulsion, 52% ~ 60% deionized water, 10% ~ 15% polyacrylamide amine emulsifier and 0.2%
Preservative it is well mixed releasing agent is made, it is standby；According to mass ratio, 1% ~ 2% NSJF-3B rubber powders, 44% ~ 60% heavy is taken
Calcium carbonate, 20% ~ 25% sierozem power and 18% ~ 30% white cement, add water stir thereto, and gypsum breast is made, standby；Take
The silicon rubber of blueness is made in molded silicon rubber and thereto addition RTV-2 crosslinking agents, standby；
Step 2: after selection anesthesia, the corpse for the pig that the fixer perfusion fixation prepared with step one is put to death；
Step 3: pig corpse is lain on one's side, cut the cm of a diameter of 0.5cm ~ 1 osculum in the ear portion median line respectively with operating scissors, connect
And cut forward to have sharp ears from cut osculum respectively；Then pig ear skin is separated, skin is sloughed to have sharp ears with the method for drill drift, made
It turns into the housing type of dermatotome outwardly, then cuts the auricular cartilage of ear, and skin is completely stripped；
Step 4: isolating ear vein in the ear after step 3 peeling, and clean out, then successively brushed in ear
Releasing agent and the gypsum breast that step one is prepared, are made pig lug die；
Step 5: inserting support tube in the ear vein that step 4 is separated, blood vessel character is propped up, then on ear vein first
Squeegeeing step one is prepared afterwards releasing agent and silicon rubber, are made ear vein silicone rubber model, and in described support tube
Internal diameter, founding mathematical models are as follows between external diameter and ear vein length：
y1=-0.01x+0.3；
y2=-0.01x+0.4；
Wherein, x represents ear vein length, y1Represent the internal diameter of support tube, y2Represent the external diameter of support tube；
Step 6: after the silicon rubber in ear vein silicone rubber model solidifies, by the institute of ear vein silicone rubber model Embedded step four
In ear vein ditch in the pig lug die of making, then brushing silica gel, and being uniformly stained with silica gel before silica gel dries
Pig hair, to form the skin model of parcel pig lug die, is positioned in specimen jar, that is, pig ear vein injection model is made.
2. a kind of pig ear vein injection model preparation method based on mathematical modeling according to claim 1, its feature exists
In：The medicament prepared in step one is matching while using or preserved under the conditions of 4 DEG C are placed in after medicament configuration.
3. a kind of pig ear vein injection model preparation method based on mathematical modeling according to claim 1, its feature exists
In：In step 4,5 times gypsum breasts are brushed after brushing releasing agent by ear.
4. a kind of pig ear vein injection model preparation method based on mathematical modeling according to claim 1, its feature exists
In：In step one, the addition of RTV-2 crosslinking agents is the 1% ~ 2% of molded silicon rubber quality.
5. a kind of pig ear vein injection model preparation method based on mathematical modeling according to claim 1, its feature exists
In：In step one, preservative used is o-phenyl phenol preservative.
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| CN2071816U (en) * | 1990-07-25 | 1991-02-20 | 中国人民解放军沈阳军区军医学校 | Model for training injection on upper extremity |
| FR2781665B1 (en) * | 1998-07-29 | 2000-09-29 | Jacques Bruguiere | PASTE COMPOSITION FOR USE AS A FILLING MATERIAL DURING A PULPOTOMY |
| CN2570905Y (en) * | 2002-09-06 | 2003-09-03 | 刘霄飞 | Simulating arm for medical use |
| CN103426354B (en) * | 2013-08-19 | 2016-08-10 | 河南科技大学 | A kind of whole-body muscle Slide processing of adult dogs |
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