CN103400529A - Integral adult cock muscle specimen producing method - Google Patents
Integral adult cock muscle specimen producing method Download PDFInfo
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- CN103400529A CN103400529A CN2013102870461A CN201310287046A CN103400529A CN 103400529 A CN103400529 A CN 103400529A CN 2013102870461 A CN2013102870461 A CN 2013102870461A CN 201310287046 A CN201310287046 A CN 201310287046A CN 103400529 A CN103400529 A CN 103400529A
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Abstract
The invention discloses an integral adult cock muscle specimen producing method. The method comprises steps of selecting an adult cock body which is anesthetized and dead in five minutes; then quickly filling 1000ml of normal saline into a cock, and sequentially perfusing plyethylene glycol aqueous solutions with the respective mass percents of 20%, 40%, 60%, 80% and 90%; skinning the cock body, and completely separating the integral cock muscles; and degreasing the integral cock muscles through adopting a mixed solution which is prepared by absolute ethyl alcohol and xylene according to a ratio of 1:1, airing for 5-7 days in dark ventilation places, soaking the aired cock nerves in 3%-5% of detergent by mass for 1-2hours, then washing with clean water for three times, bleaching with diluted hydrogen peroxide and cleaning, fixing the cock muscles on a glass plate through glue, maintaining the integral cock muscles in a standing post, and putting the integral cock muscles into a specimen jar to obtain an integral cock muscle specimen. The producing method is simple and low in cost; and the produced integral adult cock muscle specimen is easy to store, is structurally intact and attractive and has a clear and natural forming position.
Description
Technical field
The present invention relates to a kind of Slide processing, specifically a kind of whole-body muscle Slide processing of mature rooster.
Background technology
Zoological specimens, be to keep former state in kind or process to arrange referential individuality that can be used as representative in one species in the time of can supplying study, research, also can be used as the important references of the universal publicity of scientific research, teaching and Sci-tech Knowledge.The scientific value that can not be substituted, ornamental value and reserve value that animal and tissue specimen contain, other artworks of erect image equally enter into family, become the indoor new ornament of people.Make exquisite sample for one and not only bring people's knowledge, also bring simultaneously the enjoyment of people's art.Perfectly the animal muscle sample is the practical teaching aid of an image, can be again the artwork of an exquisiteness, and it represents the development level of colleges and universities, a subject and grasps this subject researcher's professional skill.Preparation of specimen is also a research work, in manufacturing process, can constantly discover problems and solve them, and creates new technology, can broaden one's knowledge simultaneously, tempers participant's thinking and manipulative ability.Be made into whole muscle specimen, can demonstrate the whole-body muscle structure, the sample structure is attractive in appearance, form is known nature, not only " Zootomy " teaching in as directly perceived, vivid teaching aid, and can allow the student participate in manufacturing process, and allow the student understand and grasp preparation of specimen's process, cultivate simultaneously student's the ability of starting, beat one's brains.Help the understanding to animal whole-body muscle overview to the student, the contour structures of whole-body muscle is grasped, the understanding of the Name ﹠ Location relation of muscle is all played good help, help to improve teaching efficiency.
Summary of the invention
The present invention seeks to as solving the problems of the technologies described above, a kind of whole-body muscle Slide processing of mature rooster is provided, its preparation method is simple, and prepared sample can demonstrate the whole-body muscle structure, and structure is attractive in appearance, and form is known nature.
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step 1, solution preparation: be 3.5% solution with the mass percent that distilled water is diluted to hydrogen peroxide with hydrogen peroxide, prepare the hydrogen peroxide after dilution, standby; Take washing powder, add distilled water, prepare mass percent and be 3%～5% detergent solution; Take respectively the Macrogol 600 of Different Weight, adding distilled water to prepare respectively mass percent is 20%, 40%, 60%, 80% and 90% Aqueous Solutions of Polyethylene Glycol; Prepared solution is wanted matching while using or is placed in 4 ℃ of preservations;
Step 2, selection anesthesia, after putting to death, the time was at five minutes corpse with interior mature rooster;
Step 3, open at the skin place, corpse abdominal cavity of cock with scalpel diameter be 0.8 ~ 1.2 cm the mouth, expose heart; Cut the osculum that diameter is 0.3 ~ 0.4 cm on right auricle of heart with operating scissors, insert the perfusion syringe needle in the left ventricle apex of the heart, fix with hemostatic forceps, quick filling 1000 ml physiological saline, after blood is rinsed well, again successively pouring quality number percent be 20%, 40%, 60%,, each 800ml of Aqueous Solutions of Polyethylene Glycol of 80% and 90%, first quick and back slow, continue approximately 2 hours;
Step 4, peeling: the cock corpse is faced upward and is put on autopsy table, cut the osculum that diameter is 0.5 ~ 1cm with operating scissors in the gambrel inboard, then from cutting osculum inboard, cut off cock skin to anus the place ahead; Then separate cock skin, with the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and beak section surrounding skin,, at the careful skin of peeling off of the apex of the wing, peel off skin is complete, gill finally;
Step 5, separation cock whole-body muscle: adopt scalpel to isolate the whole-body muscle of cock; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the pharyngeal flesh of head; At neck portion from cervical muscle, musculus hyoideus, multiple flesh and digastric muscle.At trunk, after peeling off manadesma, connective tissue, blood vessel and muscle along boundary, the terminal of muscle, separate the muscle such as flesh on intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate (flesh after the shoulder arm), latissimus dorsi, intercostal muscle, ilium tibialis anterior (sartorius), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps muscle of thigh), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis., in chicken leg section, separate ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, the inboard musculus flexor of toe and gracilis etc.;
Step 6, degreasing: with isolated cock muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have cock muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have cock muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1:1 every 12 ~ 24 hours, soak two days later and take out; Perhaps isolated cock muscle is placed in the infuse container, pours gasoline into, airtight, soak and approximately carried out degreasing in 7 days.In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 7, degreasing: the cock muscle after degreasing is placed on dark ventilation dried in the air 5～7 days, then the cock muscle mass percent that will dry well is 3%～5% detergent immersion 1～2h, then with clear water, clean three times;
Step 8, the cock muscle after above-mentioned steps seven is processed are inserted the hydrogen peroxide after dilution, soak taking-up afterwards in 2～5 days, with clear water, rinse well, dry;
Step 9, with glue, muscle is fixed on glass plate, the posture while making muscle keep cock to stand, then put in specimen jar, namely prepares the whole-body muscle sample of mature rooster.
Beneficial effect is:
1, the whole-body muscle Slide processing of mature rooster of the present invention, simple, the easy row of preparation method, with low cost; Adopt ethanol water, absolute ethyl alcohol and dimethylbenzene or gasoline to carry out ungrease treatment to cock muscle, degreasing is thorough, can prevent from using and preserving permanent, slowly oozing out of cock muscle grease, pollute mouldyly, make sample impaired, hinder and use and attractive in appearance.The mass percent that adopts hydrogen oxide is that after 3.5% dilution, hydrogen peroxide is bleached, and effect is better and to cock whole-body muscle sample not damaged.
2, the whole-body muscle Slide processing of mature rooster of the present invention, the whole-body muscle sample of prepared cock, be easy to preserve, structural integrity is attractive in appearance, morphologic localization is known nature, and whole-body muscle all clearly illustrated, each piece muscle all represents and be fixed on the physical slot of chicken body well.Solved the problem that is difficult to clear resolution cock whole-body muscle.Muscle is the emphasis in Teaching of Anatomy, and the muscle in the Systematic Anatomy teaching is mainly theoretical, and topologic Students in Teaching is not dissected whole muscle; The present invention preferably resolves this difficult problem.Excite again student's learning interest, improved teaching efficiency.
Embodiment
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step 1, solution preparation: be 3.5% solution with the mass percent that distilled water is diluted to hydrogen peroxide with hydrogen peroxide, prepare the hydrogen peroxide after dilution, standby; Take washing powder, add distilled water, prepare mass percent and be 3%～5% detergent solution; Take respectively the Macrogol 600 of Different Weight, adding distilled water to prepare respectively mass percent is 20%, 40%, 60%, 80% and 90% Aqueous Solutions of Polyethylene Glycol; Prepared solution is wanted matching while using or is placed in 4 ℃ of preservations;
Step 2, selection anesthesia, after putting to death, the time was at five minutes corpse with interior mature rooster;
Step 3, open at the skin place, corpse abdominal cavity of cock with scalpel diameter be 0.8 ~ 1.2 cm the mouth, expose heart; Cut the osculum that diameter is 0.3 ~ 0.4 cm on right auricle of heart with operating scissors, insert the perfusion syringe needle in the left ventricle apex of the heart, fix with hemostatic forceps, quick filling 1000 ml physiological saline, after blood is rinsed well, again successively pouring quality number percent be 20%, 40%, 60%,, each 800ml of Aqueous Solutions of Polyethylene Glycol of 80% and 90%, first quick and back slow, continue approximately 2 hours;
Step 4, peeling: the cock corpse is faced upward and is put on autopsy table, cut the osculum that diameter is 0.5 ~ 1cm with operating scissors in the gambrel inboard, then from cutting osculum inboard, cut off cock skin to anus the place ahead; Then separate cock skin, with the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and beak section surrounding skin,, at the careful skin of peeling off of the apex of the wing, peel off skin is complete, gill finally;
Step 5, separation cock whole-body muscle: adopt scalpel to isolate the whole-body muscle of cock; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the pharyngeal flesh of head; At neck portion from cervical muscle, musculus hyoideus, multiple flesh and digastric muscle.At trunk, after peeling off manadesma, connective tissue, blood vessel and muscle along boundary, the terminal of muscle, separate the muscle such as flesh on intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate (flesh after the shoulder arm), latissimus dorsi, intercostal muscle, ilium tibialis anterior (sartorius), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps muscle of thigh), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis., in chicken leg section, separate ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, the inboard musculus flexor of toe and gracilis etc.;
Step 6, degreasing: with isolated cock muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have cock muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have cock muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1:1 every 12 ~ 24 hours, soak two days later and take out; Perhaps isolated cock muscle is placed in the infuse container, pours gasoline into, airtight, soak and approximately carried out degreasing in 7 days.In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 7, degreasing: the cock muscle after degreasing is placed on dark ventilation dried in the air 5～7 days, then the cock muscle mass percent that will dry well is 3%～5% detergent immersion 1～2h, then with clear water, clean three times;
Step 8, the cock muscle after above-mentioned steps seven is processed are inserted the hydrogen peroxide after dilution, soak taking-up afterwards in 2～5 days, with clear water, rinse well, dry;
Step 9, with glue, muscle is fixed on glass plate, the posture while making muscle keep cock to stand, then put in specimen jar, namely prepares the whole-body muscle sample of mature rooster.
Following examples can make the present invention of those skilled in the art's complete understanding, but do not limit the present invention in any way.
Embodiment 1
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step 1, select one of plentiful and substantial, the healthy and strong mature rooster of build, claim its body weight;
Step 2, solution preparation: take yellow Jackets according to the body weight of cock, standard is that every 1 kg cock takes yellow Jackets 30mg, and it is dissolved in stroke-physiological saline solution, and to be made into massfraction be 5% amobarbital sodium solution, standby; Be 3.5% solution with the mass percent that distilled water is diluted to hydrogen peroxide with hydrogen peroxide, prepare the hydrogen peroxide after dilution, standby; Take washing powder, add distilled water, prepare mass percent and be 5% detergent solution; Take respectively the Macrogol 600 of Different Weight, adding distilled water to prepare respectively mass percent is 20%, 40%, 60%, 80% and 90% Aqueous Solutions of Polyethylene Glycol; Prepared solution is wanted matching while using or is placed in 4 ℃ of preservations;
Step 3, with syringe, draw the prepared amobarbital sodium solution of above-mentioned steps two, adopt the intramuscular anesthesia cock; With its execution;
Step 4, open at the skin place, corpse abdominal cavity of cock with scalpel diameter be 0.8 cm the mouth, expose heart; Cut the osculum that diameter is 0.4 cm on right auricle of heart with operating scissors, insert the perfusion syringe needle in the left ventricle apex of the heart, fix with hemostatic forceps, quick filling 1000 ml physiological saline, after blood is rinsed well, again successively pouring quality number percent be 20%, 40%, 60%,, each 800ml of Aqueous Solutions of Polyethylene Glycol of 80% and 90%, first quick and back slow, continue approximately 2 hours;
Step 5, peeling: the cock corpse is faced upward and is put on autopsy table, cut the osculum that diameter is 1cm with operating scissors in the gambrel inboard, then from cutting osculum inboard, cut off cock skin to anus the place ahead; Then separate cock skin, with the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and beak section surrounding skin,, at the careful skin of peeling off of the apex of the wing, peel off skin is complete, gill finally;
Step 6, separation cock whole-body muscle: adopt scalpel to isolate the whole-body muscle of cock; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the pharyngeal flesh of head; At neck portion from cervical muscle, musculus hyoideus, multiple flesh and digastric muscle.At trunk, after peeling off manadesma, connective tissue, blood vessel and muscle along boundary, the terminal of muscle, separate the muscle such as flesh on intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate (flesh after the shoulder arm), latissimus dorsi, intercostal muscle, ilium tibialis anterior (sartorius), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps muscle of thigh), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis., in chicken leg section, separate ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, the inboard musculus flexor of toe and gracilis etc.;
Step 7, degreasing: with isolated cock muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have cock muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have cock muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1:1 every 24 hours, soak two days later and take out; In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 8, degreasing: the cock muscle after degreasing is placed on dark ventilation dried in the air 7 days, then the cock muscle mass percent that will dry well is 5% detergent immersion 2h, then with clear water, clean three times;
Step 9, the cock muscle after above-mentioned steps seven is processed are inserted the hydrogen peroxide after dilution, soak taking-up afterwards in 5 days, with clear water, rinse well, dry;
Step 10, with glue, muscle is fixed on glass plate, the posture while making muscle keep cock to stand, then put in specimen jar, namely prepares the whole-body muscle sample of mature rooster.
Embodiment 2
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step 1, select one of plentiful and substantial, the healthy and strong mature rooster of build, claim its body weight;
Step 2, solution preparation: take yellow Jackets according to the body weight of cock, standard is that every 1 kg cock takes yellow Jackets 30mg, and it is dissolved in stroke-physiological saline solution, and to be made into massfraction be 3% amobarbital sodium solution, standby; Be 3.5% solution with the mass percent that distilled water is diluted to hydrogen peroxide with hydrogen peroxide, prepare the hydrogen peroxide after dilution, standby; Take washing powder, add distilled water, prepare mass percent and be 3% detergent solution; Take respectively the Macrogol 600 of Different Weight, adding distilled water to prepare respectively mass percent is 20%, 40%, 60%, 80% and 90% Aqueous Solutions of Polyethylene Glycol; Prepared solution is wanted matching while using or is placed in 4 ℃ of preservations;
Step 3, with syringe, draw the prepared amobarbital sodium solution of above-mentioned steps two, adopt the intramuscular anesthesia cock; With its execution;
Step 4, open at the skin place, corpse abdominal cavity of cock with scalpel diameter be 1.2 cm the mouth, expose heart; Cut the osculum that diameter is 0.3cm on right auricle of heart with operating scissors, insert the perfusion syringe needle in the left ventricle apex of the heart, fix with hemostatic forceps, quick filling 1000 ml physiological saline, after blood is rinsed well, again successively pouring quality number percent be 20%, 40%, 60%,, each 800ml of Aqueous Solutions of Polyethylene Glycol of 80% and 90%, first quick and back slow, continue approximately 2 hours;
Step 5, peeling: the cock corpse is faced upward and is put on autopsy table, cut the osculum that diameter is 0.5cm with operating scissors in the gambrel inboard, then from cutting osculum inboard, cut off cock skin to anus the place ahead; Then separate cock skin, with the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and beak section surrounding skin,, at the careful skin of peeling off of the apex of the wing, peel off skin is complete, gill finally;
Step 6, separation cock whole-body muscle: adopt scalpel to isolate the whole-body muscle of cock; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the pharyngeal flesh of head; At neck portion from cervical muscle, musculus hyoideus, multiple flesh and digastric muscle.At trunk, after peeling off manadesma, connective tissue, blood vessel and muscle along boundary, the terminal of muscle, separate the muscle such as flesh on intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate (flesh after the shoulder arm), latissimus dorsi, intercostal muscle, ilium tibialis anterior (sartorius), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps muscle of thigh), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis., in chicken leg section, separate ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, the inboard musculus flexor of toe and gracilis etc.;
Step 7, degreasing: with isolated cock muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have cock muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have cock muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1:1 every 12 hours, soak two days later and take out; In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 8, degreasing: the cock muscle after degreasing is placed on dark ventilation dried in the air 5 days, then the cock muscle mass percent that will dry well is 3% detergent immersion 1h, then with clear water, clean three times;
Step 9, the cock muscle after above-mentioned steps seven is processed are inserted the hydrogen peroxide after dilution, soak taking-up afterwards in 2 days, with clear water, rinse well, dry;
Step 10, with glue, muscle is fixed on glass plate, the posture while making muscle keep cock to stand, then put in specimen jar, namely prepares the whole body whole-body muscle sample of mature rooster.
Embodiment 3
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step 1, select one of plentiful and substantial, the healthy and strong mature rooster of build, claim its body weight;
Step 2, solution preparation: take yellow Jackets according to the body weight of cock, standard is that every 1 kg cock takes yellow Jackets 30mg, and it is dissolved in stroke-physiological saline solution, and to be made into massfraction be 4% amobarbital sodium solution, standby; Be 3.5% solution with the mass percent that distilled water is diluted to hydrogen peroxide with hydrogen peroxide, prepare the hydrogen peroxide after dilution, standby; Take washing powder, add distilled water, prepare mass percent and be 4% detergent solution; Take respectively the Macrogol 600 of Different Weight, adding distilled water to prepare respectively mass percent is 20%, 40%, 60%, 80% and 90% Aqueous Solutions of Polyethylene Glycol; Prepared solution is wanted matching while using or is placed in 4 ℃ of preservations;
Step 3, with syringe, draw the prepared amobarbital sodium solution of above-mentioned steps two, adopt the intramuscular anesthesia cock; With its execution;
Step 4, open at the skin place, corpse abdominal cavity of cock with scalpel diameter be 1.0cm the mouth, expose heart; Cut the osculum that diameter is 0.35cm on right auricle of heart with operating scissors, insert the perfusion syringe needle in the left ventricle apex of the heart, fix with hemostatic forceps, quick filling 1000 ml physiological saline, after blood is rinsed well, again successively pouring quality number percent be 20%, 40%, 60%,, each 800ml of Aqueous Solutions of Polyethylene Glycol of 80% and 90%, first quick and back slow, continue approximately 2 hours;
Step 5, peeling: the cock corpse is faced upward and is put on autopsy table, cut the osculum that diameter is 0.8cm with operating scissors in the gambrel inboard, then from cutting osculum inboard, cut off cock skin to anus the place ahead; Then separate cock skin, with the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and beak section surrounding skin,, at the careful skin of peeling off of the apex of the wing, peel off skin is complete, gill finally;
Step 6, separation cock whole-body muscle: adopt scalpel to isolate the whole-body muscle of cock; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the pharyngeal flesh of head; At neck portion from cervical muscle, musculus hyoideus, multiple flesh and digastric muscle.At trunk, after peeling off manadesma, connective tissue, blood vessel and muscle along boundary, the terminal of muscle, separate the muscle such as flesh on intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate (flesh after the shoulder arm), latissimus dorsi, intercostal muscle, ilium tibialis anterior (sartorius), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps muscle of thigh), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis., in chicken leg section, separate ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, the inboard musculus flexor of toe and gracilis etc.;
Step 7, degreasing: with isolated cock muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have cock muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have cock muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1:1 every 18 hours, soak two days later and take out; In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 8, degreasing: the cock muscle after degreasing is placed on dark ventilation dried in the air 6 days, then the cock muscle mass percent that will dry well is 4% detergent immersion 1.5h, then with clear water, clean three times;
Step 9, the cock muscle after above-mentioned steps seven is processed are inserted the hydrogen peroxide after dilution, soak taking-up afterwards in 3 days, with clear water, rinse well, dry;
Step 10, with glue, muscle is fixed on glass plate, the posture while making muscle keep cock to stand, then put in specimen jar, namely prepares the whole body whole-body muscle sample of mature rooster.
Embodiment 4
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step 1, select one of plentiful and substantial, the healthy and strong mature rooster of build, claim its body weight;
Step 2, solution preparation: take yellow Jackets according to the body weight of cock, standard is that every 1 kg cock takes yellow Jackets 30mg, and it is dissolved in stroke-physiological saline solution, and to be made into massfraction be 4% amobarbital sodium solution, standby; Be 3.5% solution with the mass percent that distilled water is diluted to hydrogen peroxide with hydrogen peroxide, prepare the hydrogen peroxide after dilution, standby; Take washing powder, add distilled water, prepare mass percent and be 4% detergent solution; Take respectively the Macrogol 600 of Different Weight, adding distilled water to prepare respectively mass percent is 20%, 40%, 60%, 80% and 90% Aqueous Solutions of Polyethylene Glycol; Prepared solution is wanted matching while using or is placed in 4 ℃ of preservations;
Step 3, with syringe, draw the prepared amobarbital sodium solution of above-mentioned steps two, adopt the intramuscular anesthesia cock; With its execution;
Step 4, open at the skin place, corpse abdominal cavity of cock with scalpel diameter be 1.0cm the mouth, expose heart; Cut the osculum that diameter is 0.35cm on right auricle of heart with operating scissors, insert the perfusion syringe needle in the left ventricle apex of the heart, fix with hemostatic forceps, quick filling 1000 ml physiological saline, after blood is rinsed well, again successively pouring quality number percent be 20%, 40%, 60%,, each 800ml of Aqueous Solutions of Polyethylene Glycol of 80% and 90%, first quick and back slow, continue approximately 2 hours;
Step 5, peeling: the cock corpse is faced upward and is put on autopsy table, cut the osculum that diameter is 0.8cm with operating scissors in the gambrel inboard, then from cutting osculum inboard, cut off cock skin to anus the place ahead; Then separate cock skin, with the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and beak section surrounding skin,, at the careful skin of peeling off of the apex of the wing, peel off skin is complete, gill finally;
Step 6, separation cock whole-body muscle: adopt scalpel to isolate the whole-body muscle of cock; Concrete operation method is: peel off under head, neck and trunk skin and isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis.Wipe out manadesma and connective tissue along muscle distribution position, isolate facial muscle, masseter and the pharyngeal flesh of head; At neck portion from cervical muscle, musculus hyoideus, multiple flesh and digastric muscle.At trunk, after peeling off manadesma, connective tissue, blood vessel and muscle along boundary, the terminal of muscle, separate the muscle such as flesh on intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate (flesh after the shoulder arm), latissimus dorsi, intercostal muscle, ilium tibialis anterior (sartorius), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps muscle of thigh), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis., in chicken leg section, separate ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, the inboard musculus flexor of toe and gracilis etc.;
Step 7, degreasing: isolated cock muscle is placed in the infuse container, pours gasoline into, airtight, soak and approximately carried out degreasing in 7 days.In the degreasing operation process,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation, in order to avoid operating personnel are damaged;
Processing after step 8, degreasing: the cock muscle after degreasing is placed on dark ventilation dried in the air 6 days, then the cock muscle mass percent that will dry well is 4% detergent immersion 1.5h, then with clear water, clean three times;
Step 9, the cock muscle after above-mentioned steps seven is processed are inserted the hydrogen peroxide after dilution, soak taking-up afterwards in 3 days, with clear water, rinse well, dry;
Step 10, with glue, muscle is fixed on glass plate, the posture while making muscle keep cock to stand, then put in specimen jar, namely prepares the whole-body muscle sample of mature rooster.
Claims (3)
1. the whole-body muscle Slide processing of a mature rooster, it is characterized in that: its concrete preparation process is:
Step 1, solution preparation: be 3.5% solution with the mass percent that distilled water is diluted to hydrogen peroxide with hydrogen peroxide, prepare the hydrogen peroxide after dilution, standby; Take washing powder, add distilled water, prepare mass percent and be 3%～5% detergent solution; Take respectively the Macrogol 600 of Different Weight, adding distilled water to prepare respectively mass percent is 20%, 40%, 60%, 80% and 90% Aqueous Solutions of Polyethylene Glycol; Prepared solution is wanted matching while using or is placed in 4 ℃ of preservations;
After step 2, selection anesthesia are put to death, the time was at five minutes corpse with interior mature rooster;
Step 3, open at the skin place, corpse abdominal cavity of cock with scalpel diameter be 0.8 ~ 1.2cm the mouth, expose heart; Cut the osculum that diameter is 0.3 ~ 0.4 cm on right auricle of heart with operating scissors, insert the perfusion syringe needle in the left ventricle apex of the heart, fix with hemostatic forceps, pour into 1000 ml physiological saline, after blood is rinsed well, again successively pouring quality number percent be 20%, 40%, 60%,, each 800ml of Aqueous Solutions of Polyethylene Glycol of 80% and 90%, continue approximately 2 hours;
Step 4, peeling: the cock corpse is faced upward and is put on autopsy table, cut the osculum that diameter is 0.5 ~ 1cm with operating scissors in the gambrel inboard, then from cutting osculum inboard, cut off cock skin to anus the place ahead; Then separate cock skin, with the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and beak section surrounding skin,, at the careful skin of peeling off of the apex of the wing, peel off skin is complete, gill finally;
Step 5, separation cock whole-body muscle: adopt scalpel to isolate the whole-body muscle of cock;
Step 6, degreasing: with isolated cock muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have cock muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have cock muscle fully, changed and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1:1 every 12 ~ 24 hours, soak two days later and take out;
Processing after step 7, degreasing: the cock muscle after degreasing is placed on dark ventilation dried in the air 5～7 days, then the cock muscle mass percent that will dry well is 3%～5% detergent immersion 1～2h, then with clear water, clean three times;
Step 8, the cock muscle after above-mentioned steps seven is processed are inserted the hydrogen peroxide after dilution, soak taking-up afterwards in 2～5 days, with clear water, rinse well, dry;
Step 9, with glue, muscle is fixed on glass plate, the posture while making muscle keep cock to stand, then put in specimen jar, namely prepares the whole-body muscle sample of mature rooster.
2. the whole-body muscle Slide processing of a kind of mature rooster as claimed in claim 1 is characterized in that: in the operating process of described step 6 degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
3. the whole-body muscle Slide processing of a kind of mature rooster as claimed in claim 1, it is characterized in that: the degreasing method in described step 6 also can adopt: isolated cock muscle specimen is placed in the infuse container, pour gasoline into, airtight, soak and approximately carried out degreasing in 7 days.
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