CN107274770A - A kind of preparation method of crystal heart efferent tract sample - Google Patents
A kind of preparation method of crystal heart efferent tract sample Download PDFInfo
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- CN107274770A CN107274770A CN201710094825.8A CN201710094825A CN107274770A CN 107274770 A CN107274770 A CN 107274770A CN 201710094825 A CN201710094825 A CN 201710094825A CN 107274770 A CN107274770 A CN 107274770A
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- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/36—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for zoology
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Abstract
The invention belongs to animal tissue specimens manufacture technology field, and in particular to a kind of preparation method of crystal heart efferent tract sample.The present invention is first that zoological specimens are fixed successively for 30~50% ethanol, 75~100% methanol, 75~100% acetone and 95~100% ethanol with percent by volume；Then transparent processing is carried out to it using the glycerine of various concentrations gradient.Tissue specimen made from the inventive method can not only clearly, silk hair do not damage, and ecosystem cardiac system is showed；And need not will not be caused environment pollution, with traditional dipped into formalin while fabrication cycle is shorten to 15~30 days；It can be used for the scientific research that cardiac system develops field, with the value such as extensive art appreciation and use in teaching.
Description
Technical field
The invention belongs to animal tissue specimens manufacture technology field, and in particular to a kind of system of crystal heart efferent tract sample
Make method.
Background technology
The sample of animal tissue is played an important role in teaching and scientific research field.Made on domestic market
Most of zoological specimens be the common sample fixed using formalin, the sample of conventional cardiac is all that volume ratio is larger, or
Person is the layering of displaying cardiac muscle, or shows each chamber, and the related efferent tract displaying of heart is sufficiently complete and whole
Individual sample is immersed in formalin solution, over time can blackening, student seen except nausea is exactly the sense of disagreeable study
Feel.Make heart efferent tract, its appearance looks elegant degree is poor, and can not heart efferent tract visible in detail development situation,
It is not easy to carry out research and teaching for various heart developments deformity.Especially in embryonic stage heart or heart pipe, now
Heart pipe study only by the model on textbook, almost teachers Lian Jiaoxue did not meet what sample real heart pipe is yet
Son, it is unfavorable for very much teaching and scientific research.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of making side of crystal heart efferent tract sample
Method.A kind of brand-new clearing method is used in the preparation method, the crystal heart efferent tract not only produced is superior in quality,
The configuration of efferent tract can be more clearly observed, is easy to the judgement to various heart developments deformity, and fabrication cycle is short.
For achieving the above object, the present invention is achieved through the following technical solutions：
A kind of preparation method of crystal heart efferent tract sample, including following preparation process：
(1) draw materials：Obtain zoological specimens；
(2) it is fixed：Use successively concentration of volume percent for 30~50% ethanol, 75~100% methanol and 95~
The zoological specimens obtained in 100% ethanol treatment step (1)；Wherein, the effect of acetone is further preferably degreasing；Second
Its effect of secondary use ethanol is primarily to elution acetone, further dehydration, is that subsequent experimental is prepared.In fixation procedure, choosing
Use concentration of volume percent as described above, on the one hand, acutely tissue will not be made to shrink in the interval concentration, and make zoomorphism
Change；On the other hand, the dissolving lipid that other concentration can not be optimal.
(3) transparency process：It is first the sample after 1~5% KOH elution steps (2) processing with concentration of volume percent,
Use the animal mark of 30~50% glycerine, 60~75% glycerine and 90~100% glycerine pair successively again
This progress gradient transparency process.Gradient transparent processing effectively can prevent tissue shrinkage from deforming, and lose natural form.At transparence
Reason different animals tissue treatment number of days differs, according to being bottom that animal tissue sinks to glycerine, and transparency increase,
(4) microscopic processing：Under the microscope, corresponding site is removed to the heart lung preparation after step (3) transparency process
Soft tissue, obtains the crystal heart efferent tract sample.
It is preferred that in step (2), first being entered before described 95~100% Ethanol Treatment with 75~100% acetone to sample
Row processing.
It is preferred that in the step (3), described 30~50% glycerine before processing, first with 1~5% KOH from low dense
Spend high concentration gradually elution step (1) sample.
It is preferred that it is described with 1~5% KOH from low concentration to high concentration gradually after elution step (1) described sample,
First sample is handled with hydrogen peroxide, after again with 30~50% glycerine handle.
It is preferred that the step (2) is specially：Gained zoological specimens in step (1) are first entered in 30~50% ethanol
It is placed on shaking table and fixes 10~15 hours；It is placed in the methanol for placing into 75~100% on shaking table 10~15 hours；Place into 75
It is placed in~100% acetone on shaking table 10~15 hours；Finally zoological specimens are put into be placed in 95~100% ethanol and shaken
Upper 10~15 hours of bed.The fixing process time of different animals tissue is different, and the tissue of other animals is that have hairless according to skin
Hair, subcutaneus adipose tissue are how many and compactness extent of skin is come what is determined, and different animals processing mode is distinguishing.
It is preferred that the shaking table is reported as 50~70 revs/min.
The picture shooting method of the crystal heart efferent tract sample made of methods described, when shooting, uses targets improvement
Method is taken pictures；It is specifically that the transparent glass plate for placing sample will be traditional, light source is placed on transparent when shooting
Glass plate bottom, is changed to light source being provided in sample with two sides.Substantially transparent rear Stereo microscope or slr camera are taken pictures,
The clear structure of clearly heart and its each efferent tract can be preserved.
The present invention is fixed using 30~50% ethanol, 75~100% methanol, 75~100% acetone and 95~100% ethanol
Zoological specimens；After fully fixing, 1~5%KOH gradually elutes sample from low concentration to high concentration, softens it, then using difference
The glycerine of gradient carries out gradually transparency process to it；Substantially transparent rear Stereo microscope or slr camera are taken pictures, not only
Fabrication cycle is shorten to 15~30 days, and can preserve the clear structure of clearly heart and its each efferent tract.
It is relatively conventional that whole heart is taken out, it is soaked in the preparation method in formalin solution, the present invention is not only not
The normal neighbouring relationship of heart and its surrounding tissue can be destroyed, and bandwagon effect is true and vivid, is especially advantageous for Students ' Major
Study.The displaying sample of conventional heart and its each dependency structure is all larger embryo or adult, the embryo's less than 8cm
Heart is operated due to too small using traditional dissection, it is impossible to which the structure of correct display heart and its correlation (can only pass through pattern
Figure).In embryonic stage heart or heart pipe, the study of heart pipe now almost connects religion only by the model on textbook
Learn teachers and also do not met what appearance real heart pipe is, the technology of the present invention is broken the normal procedure, can be in situ, clear, lossless
Any heart and its each structure of efferent tract of the displaying from the formation of heart pipe to adult, and each anatomical structure it is coherent,
Neighbouring relationship is able to sufficiently show, medical specimen has been reached attractive in appearance, sophistication, start the medical science artistic epoch.
Crystal heart efferent tract is that one kind can quickly make animal hearts efferent tract with similar " 4D X-ray " by the present invention
The method that shows of effect so that can not usually observe fine cardiac system can clearly, silk hair do not damage and former
Show ecologically.Different from traditional heart efferent tract sample, the crystal heart efferent tract art work of the invention is presented saturating
Magical effect as bright, without traditional dipped into formalin, and is preserved using environment-protecting and non-poisonous solution, can be not only used for
Cardiac system develops the scientific research in field, is also worth with extensive art appreciation and use in teaching etc..
Brief description of the drawings
Fig. 1 shoots schematic diagram for tradition；
Fig. 2 shoots schematic diagram for the present invention；
Fig. 3 shoots the schematic diagram of sample for tradition；
Fig. 4 shoots the schematic diagram of sample for the present invention.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, is managed to help those skilled in the art
The solution present invention.
Embodiment 1
The method for making chicken embryo crystal heart efferent tract sample, is comprised the following steps that：
12 days chicken embryos of hatching are taken, is put into 30% ethanol and fixes, put shaking table 12 hours；It is then placed in 80% methanol fixed
12 hours；Place into and 12 hours are fixed in 80% acetone；Finally chicken embryo is put into 95% ethanol and fixes 12 hours；
Be put into 30% glycerine about 48 hours, after sequentially enter 75% glycerine, 100% glycerine carry out gradient it is transparent
Change is handled about 2 days until embryo sinks to bottom；Microscopic processing is carried out to the chicken embryo after dyeing, under the microscope, to transparence
Zoological specimens after processing, open chest, remove the soft tissue of corresponding site, obtain the crystal heart efferent tract sample.
Embodiment 2
Human embryos crystal heart efferent tract method is made, is comprised the following steps that：
Take human embryos to be put into 30% ethanol fixed, put shaking table 12 hours, be then placed in 75% methanol that to fix 12 small
When；Place into and 12 hours are fixed in 95% acetone (being less than 12 weeks people's embryos can omit)；Human embryos are finally put into 100% second
12 hours are fixed in alcohol；
Be put into 30% glycerine about 48 hours, after sequentially enter 60% glycerine, 95-100% glycerine carry out gradient
Transparency process about 6 days is until embryo sinks to container bottom；Microscopic processing completion making is carried out to the human embryos after transparent.
Embodiment 3
Mouse crystal heart efferent tract method is made, is comprised the following steps that：
Take mouse to be put into 50% ethanol fixed, put shaking table 12 hours, be then placed in 100% methanol and fix 12 hours；Again
It is put into 100% acetone and fixes 12 hours；Finally mouse is put into 100% ethanol and fixes 12 hours；
Be put into 50% glycerine about 48 hours, after sequentially enter 75% glycerine, 100% glycerine carry out gradient it is transparent
Change is handled about 6 days until sinking to container bottom；Microscopic processing completion making is carried out to the mouse after transparent.
Embodiment 4
The method for making tortoise crystal heart efferent tract sample, is comprised the following steps that：
Trachemys scripta one or several is taken, is put into 50% ethanol and fixes, put shaking table 12 hours；It is then placed in 100% methanol
In fix 12 hours；Place into and 12 hours are fixed in 100% acetone；Trachemys scripta is finally put into 100% ethanol to fix 12 small
When；
250 microlitre of 30% hydrogen peroxide is added in 1000mL pure water, Trachemys scripta is put into, rinsed 5 days；Remove Trachemys scripta
Inherent color.
Be put into 50% glycerine about 48 hours, after sequentially enter 75% glycerine, 100% glycerine carry out gradient it is transparent
Change is handled about 2 days until tortoise sinks to container bottom；Microscopic processing completion making is carried out to the Trachemys scripta after transparent.
Embodiment 5
The preparation method of bat crystal heart efferent tract, is comprised the following steps that：
Bat one is taken, row peeling art is put into 50% ethanol and fixed, puts shaking table 12 hours；It is then placed in 100% methanol
In fix 12 hours；Place into and 12 hours are fixed in 100% acetone；Finally bat is put into 100% ethanol and fixes 12 hours；
250 microlitre of 30% hydrogen peroxide is added in 1000mL pure water, bat is put into, rinses 24 hours, removes bat
The color of body intracorporeal heart peripheral blood, is easy to observation.
It is put into 50% glycerine about 48 hours；Carry out microscopic processing in advance afterwards, sequentially entered after removing unnecessary soft tissue
75% glycerine, 100% glycerine carry out gradient transparency process about 3 days until sinking to container bottom；To the bat after transparent
Bat carries out microscopic processing and completes to make.
Embodiment 6
Mouse crystal heart efferent tract method is made, is comprised the following steps that：
Take mouse to be put into 30% ethanol fixed, put shaking table 12 hours, be then placed in 100% methanol and fix 12 hours；Again
It is put into 75% acetone and fixes 12 hours；Finally mouse is put into 100% ethanol and fixes 12 hours；
Be put into 30% glycerine about 48 hours, after sequentially enter 60% glycerine, 90% glycerine carry out gradient it is transparent
Change is handled about 6 days until sinking to container bottom；Microscopic processing completion making is carried out to the mouse after transparent.
Embodiment 7
Mouse crystal heart efferent tract method is made, is comprised the following steps that：
Take mouse to be put into 50% ethanol fixed, put shaking table 12 hours, be then placed in 75% methanol and fix 12 hours；
Place into and 12 hours are fixed in 75% acetone；Finally mouse is put into 95% ethanol and fixes 12 hours；It is put into 50%
About 48 hours in glycerine, after sequentially enter 75% glycerine, 100% glycerine to carry out gradient transparency process about 6 days straight
To sinking to container bottom；Microscopic processing completion making is carried out to the mouse after transparent.
Embodiment 8
Mouse crystal heart efferent tract method is made, is comprised the following steps that：
Take mouse to be put into 45% ethanol fixed, put shaking table 12 hours, be then placed in 100% methanol and fix 12 hours；Again
It is put into 90% acetone and fixes 12 hours；Finally mouse is put into 100% ethanol and fixes 12 hours；
Be put into 45% glycerine about 48 hours, after sequentially enter 70% glycerine, 95% glycerine carry out gradient it is transparent
Change is handled about 6 days until sinking to container bottom；Microscopic processing completion making is carried out to the mouse after transparent.
Above-described embodiment, simply presently preferred embodiments of the present invention, not for limiting the scope of the present invention, thus it is all with this
The equivalent change or modification that feature and principle described in invention claim are done, all should be included in scope of the invention as claimed
Within.
Crystal heart efferent tract obtained by the present invention, with heart efferent tract is fine, attractive in appearance, transparency is high, fabrication cycle
It is short, can volume production the features such as, be fully to prove, relevant survey data is as follows.
1. experiment
1.1 crystal heart efferent tracts make：Set 4 groups altogether, respectively 1 scheme group, 2 scheme groups, 3 scheme groups and control group,
Control group is traditional sample；
1.2 comparative approach：From crowd, 100 people are selected at random, investigate the favorable rating of its sample different to two kinds；
1.3 statistical analysis carry out statistical analysis, P with SPSS 13.0<0.05 indicates significant.
2. result, is statistically analyzed, each scheme group of the invention is compared with control group with notable difference, is as a result shown,
The more traditional sample of the present invention is more easy to be received by crowd.
Claims (9)
1. a kind of preparation method of crystal heart efferent tract sample, it is characterised in that including following preparation process：
(1) draw materials：Obtain zoological specimens；
(2) it is fixed：Concentration of volume percent is used successively for 30~50% ethanol, 75~100% methanol and 95~100%
Ethanol treatment step (1) in obtain zoological specimens；
(3) transparency process：Sample after first being fixed with concentration of volume percent for 1%~5% KOH elution steps (2), then
Successively gradient transparency process is carried out using the zoological specimens of the glycerine pair of different volumes percent concentration from low to high；
(4) microscopic processing：Under the microscope, corresponding site is removed to the heart lung preparation obtained after step (3) transparency process
Soft tissue, obtains the crystal heart efferent tract sample.
2. the preparation method of crystal heart efferent tract sample as claimed in claim 1, it is characterised in that in step (2), described 95
First the zoological specimens are handled with 75~100% acetone before~100% Ethanol Treatment.
3. the preparation method of crystal heart efferent tract sample as claimed in claim 1, it is characterised in that in step (3), then successively
Gradient is carried out using the zoological specimens of 30~50% glycerine, 60~75% glycerine and 90~100% glycerine pair
Transparency process.
4. the preparation method of crystal heart efferent tract sample as claimed in claim 1, it is characterised in that described in step (3)
KOH is eluted, and is gradually eluted from low concentration to high concentration.
5. the preparation method of crystal heart efferent tract sample as claimed in claim 4, it is characterised in that in step (3), described to use
1~5% KOH gradually after elution step (2) described sample, is first carried out from low concentration to high concentration with hydrogen peroxide to sample
Processing, after again with 30~50% glycerine processing.
6. the preparation method of crystal heart efferent tract sample as claimed in claim 1, it is characterised in that the step (2) is specific
For：Gained zoological specimens in step (1) are first put into 30~50% ethanol and are placed on shaking table 10~15 hours；Place into 75
It is placed in~100% methanol on shaking table 10~15 hours；10~15 are placed on shaking table in the acetone for placing into 75~100% small
When；Finally zoological specimens are put into 95~100% ethanol to be placed on shaking table and fix 10~15 hours.
7. the preparation method of crystal heart efferent tract sample as claimed in claim 6, it is characterised in that the shaking table is reported as 50
~70 revs/min.
8. the image pickup method of obtained crystal heart efferent tract sample is made of any one methods described in claim 1-7,
Characterized in that, standby targets improvement method is taken pictures when shooting.
9. method as claimed in claim 8 makes the image pickup method of obtained crystal heart efferent tract sample, it is characterised in that clap
Tradition is penetrated into light source when taking the photograph and is changed to side light source.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710094825.8A CN107274770A (en) | 2017-02-22 | 2017-02-22 | A kind of preparation method of crystal heart efferent tract sample |
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| CN201710094825.8A CN107274770A (en) | 2017-02-22 | 2017-02-22 | A kind of preparation method of crystal heart efferent tract sample |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110001267A (en) * | 2019-04-25 | 2019-07-12 | 广东海洋大学 | A kind of pearl shell shell and preparation method thereof of the stratum corneal edge without cracking phenomena |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1320369A (en) * | 2001-04-03 | 2001-11-07 | 中山医科大学 | Method for making animal skeleton specimen |
| CN201392611Y (en) * | 2009-03-30 | 2010-01-27 | 昌吉回族自治州卫生学校 | Polyester resin embedded herbarium |
| CN104872110A (en) * | 2015-05-28 | 2015-09-02 | 暨南大学 | Crystal skeleton specimen manufacturing method |
-
2017
- 2017-02-22 CN CN201710094825.8A patent/CN107274770A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1320369A (en) * | 2001-04-03 | 2001-11-07 | 中山医科大学 | Method for making animal skeleton specimen |
| CN201392611Y (en) * | 2009-03-30 | 2010-01-27 | 昌吉回族自治州卫生学校 | Polyester resin embedded herbarium |
| CN104872110A (en) * | 2015-05-28 | 2015-09-02 | 暨南大学 | Crystal skeleton specimen manufacturing method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110001267A (en) * | 2019-04-25 | 2019-07-12 | 广东海洋大学 | A kind of pearl shell shell and preparation method thereof of the stratum corneal edge without cracking phenomena |
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Application publication date: 20171020 |
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